FastQCFastQC Report
Fri 27 May 2016
SRR939085_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939085_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2105406
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55140.261897230272926No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49050.23297169287063874No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36700.17431317285122203No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29840.14173038359347317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73000.026.0965541
GTATCAA96300.024.3818511
GTACATG71800.023.8131071
TACATGG72650.022.7294062
ACATGGG74450.021.4787833
GAGTACT59350.021.38739212-13
ATACCGG1150.003746673720.6308276
ATCAACG112500.020.5831473
TCAACGC113300.020.5215744
CAACGCA113400.020.4616325
GTACTTT62100.020.2874614-15
TATCAAC115600.020.1953662
AACGCAG116350.019.983626
AGAGTAC94150.019.15217210-11
CATGGGG42050.018.5064164
AGTACTT63200.018.32008612-13
ACGCAGA130200.017.8578647
CGCAGAG131000.017.6039228
GTCTAGC5150.017.5708371
TAAGGTA6500.017.5203324
Produced by FastQC (version 0.11.5)