##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939085_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2105406 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.511745478069315 34.0 31.0 34.0 31.0 34.0 2 32.72241363423492 34.0 31.0 34.0 31.0 34.0 3 32.815670706742544 34.0 31.0 34.0 31.0 34.0 4 36.26873201653268 37.0 37.0 37.0 35.0 37.0 5 36.22659905025444 37.0 37.0 37.0 35.0 37.0 6 36.26065186477098 37.0 37.0 37.0 35.0 37.0 7 36.21431495873005 37.0 37.0 37.0 35.0 37.0 8 36.1986937436295 37.0 37.0 37.0 35.0 37.0 9 37.95113769030771 39.0 38.0 39.0 35.0 39.0 10-11 37.88854928693088 39.0 38.0 39.0 35.0 39.0 12-13 37.820974434384624 39.0 38.0 39.0 35.0 39.0 14-15 39.20390247771689 40.5 39.0 41.0 36.0 41.0 16-17 39.13836713678977 40.0 39.0 41.0 36.0 41.0 18-19 39.151629424443556 40.5 38.5 41.0 36.0 41.0 20-21 39.218459290037174 41.0 39.0 41.0 36.0 41.0 22-23 39.17995009038637 41.0 39.0 41.0 36.0 41.0 24-25 39.122981268220954 41.0 39.0 41.0 35.5 41.0 26-27 38.990337968068864 40.0 38.5 41.0 35.0 41.0 28-29 38.83436733817611 40.0 38.0 41.0 35.0 41.0 30-31 38.65743210573163 40.0 38.0 41.0 34.5 41.0 32-33 38.52776471616401 40.0 38.0 41.0 34.0 41.0 34-35 38.48991334687941 40.0 38.0 41.0 34.0 41.0 36-37 38.387400577370826 40.0 38.0 41.0 34.0 41.0 38-39 38.25718649989598 40.0 38.0 41.0 34.0 41.0 40-41 38.09165286885285 40.0 38.0 41.0 33.0 41.0 42-43 37.93342994177845 40.0 38.0 41.0 33.0 41.0 44-45 37.72303322969536 40.0 37.0 41.0 33.0 41.0 46-47 37.45036444277255 40.0 37.0 41.0 32.5 41.0 48-49 37.14742073500313 40.0 37.0 41.0 31.5 41.0 50-51 36.88920759226487 40.0 36.0 41.0 31.0 41.0 52-53 36.64678499063838 39.0 36.0 41.0 31.0 41.0 54-55 36.39206286103487 39.0 35.0 41.0 30.0 41.0 56-57 36.09519304115216 39.0 35.0 40.5 30.0 41.0 58-59 35.775107508955514 38.5 35.0 40.0 29.0 41.0 60-61 35.39444529938643 38.0 35.0 40.0 28.0 41.0 62-63 34.98531898360696 37.5 34.0 40.0 28.0 41.0 64-65 34.56000647856043 37.0 34.0 39.5 27.0 41.0 66-67 34.1852742416427 36.0 34.0 39.0 26.5 41.0 68-69 33.704516136080166 36.0 33.0 39.0 26.0 40.0 70-71 33.247622786293945 35.0 33.0 38.5 26.0 40.0 72-73 32.76384744795065 35.0 32.5 37.5 24.5 39.5 74-75 32.26529847449851 35.0 32.0 37.0 23.5 39.0 76-77 30.315636271578974 33.5 29.5 35.0 20.0 37.5 78-79 31.286172358205498 34.5 31.0 36.0 21.0 38.0 80-81 31.320503266353377 35.0 31.0 35.5 21.5 37.0 82-83 31.0368052527636 34.5 31.0 35.0 20.0 37.0 84-85 30.66956183272965 34.0 31.0 35.0 19.0 36.5 86-87 30.123195716170656 34.0 30.5 35.0 14.5 36.0 88-89 29.588088948164867 34.0 30.0 35.0 7.0 35.5 90-91 29.137246450328345 34.0 29.0 35.0 3.5 35.0 92-93 28.856206831366492 34.0 29.0 35.0 2.0 35.0 94-95 28.518909416996056 34.0 29.0 35.0 2.0 35.0 96-97 27.953950924429776 34.0 29.0 35.0 2.0 35.0 98-99 27.167277475223308 33.5 27.0 35.0 2.0 35.0 100-101 23.478352868757852 29.5 13.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1701209667641308 1101 2 0.15956895457407683 1101 3 0.19200332706017775 1101 4 0.11231200458701807 1101 5 0.21383463240009348 1101 6 0.15619732353726334 1101 7 0.13534586773809565 1101 8 0.16465857101719905 1101 9 0.24920659915443366 1101 10-11 0.215308774068248 1101 12-13 0.11509368561141287 1101 14-15 0.12053250867349874 1101 16-17 0.23881212636934634 1101 18-19 0.20412602011199255 1101 20-21 0.22725270630747474 1101 22-23 0.1865213426214254 1101 24-25 0.1735952635712792 1101 26-27 0.1823003362268949 1101 28-29 0.12057363632412432 1101 30-31 0.19930907254327224 1101 32-33 0.16809186820002964 1101 34-35 0.2836852320500114 1101 36-37 0.36017507522526415 1101 38-39 0.32512826672979145 1101 40-41 0.2758196417947545 1101 42-43 0.3533666342362878 1101 44-45 0.42964883493339556 1101 46-47 0.3726890656027919 1101 48-49 0.22119154860282464 1101 50-51 0.3152598118211358 1101 52-53 0.24055176028379321 1101 54-55 0.22975948198855178 1101 56-57 0.25803850397038985 1101 58-59 0.14967527539113945 1101 60-61 0.026919358248790104 1101 62-63 0.05298479662124578 1101 64-65 -0.060689821191488136 1101 66-67 -0.13410440851261285 1101 68-69 -0.1819942112648576 1101 70-71 -0.06308490017208612 1101 72-73 -0.07727563220552014 1101 74-75 -0.09293990398072083 1101 76-77 0.0032983495326170953 1101 78-79 0.1054511043313191 1101 80-81 0.03187690853145142 1101 82-83 -0.04478561363115219 1101 84-85 0.0014824081478330697 1101 86-87 0.033556021434076655 1101 88-89 0.1406981078815157 1101 90-91 0.2314178860014664 1101 92-93 0.07442613594457015 1101 94-95 -0.074040749396179 1101 96-97 -0.18588756311708465 1101 98-99 -0.4450086150849444 1101 100-101 -0.6289186055092912 1102 1 0.2772939268693193 1102 2 0.14677468536174842 1102 3 0.22127302773614588 1102 4 0.11790517872208284 1102 5 0.1336225559371087 1102 6 0.17272261075451212 1102 7 0.21818368055743775 1102 8 0.14207420618970446 1102 9 0.3045426954489301 1102 10-11 0.2551761752569064 1102 12-13 0.26284266918087695 1102 14-15 0.3441873144328582 1102 16-17 0.28391234080739736 1102 18-19 0.26267883295320615 1102 20-21 0.36610768583309294 1102 22-23 0.3643409260197501 1102 24-25 0.28467548614369065 1102 26-27 0.3062146251482787 1102 28-29 0.2556952654119016 1102 30-31 0.3254799903118908 1102 32-33 0.28670541176332165 1102 34-35 0.3527809332303704 1102 36-37 0.4000255662752821 1102 38-39 0.3914754872715278 1102 40-41 0.4655847803777178 1102 42-43 0.45717797539979443 1102 44-45 0.43514958641412704 1102 46-47 0.41796873159839976 1102 48-49 0.4978259645251768 1102 50-51 0.48994577159541564 1102 52-53 0.46396749166915185 1102 54-55 0.4926214646439959 1102 56-57 0.4140182059729369 1102 58-59 0.3445143302310072 1102 60-61 0.3199309518981579 1102 62-63 0.37530005700093483 1102 64-65 0.3482987921625593 1102 66-67 0.40104783375719677 1102 68-69 0.2792162631082675 1102 70-71 0.19991003115963224 1102 72-73 0.23007371813903177 1102 74-75 0.11756887252701631 1102 76-77 0.13984757334267783 1102 78-79 0.2259686420402005 1102 80-81 0.1187508507366779 1102 82-83 0.02689136190308261 1102 84-85 0.18976280740203677 1102 86-87 0.29618782211602124 1102 88-89 0.3632862629124993 1102 90-91 0.48071225215552715 1102 92-93 0.5675625147550001 1102 94-95 0.3271347967940095 1102 96-97 0.23124197000273483 1102 98-99 0.1902000429060422 1102 100-101 -0.47009289801678733 1103 1 0.11651232533515099 1103 2 0.10109246907703096 1103 3 0.13634294970168526 1103 4 0.10687691587961012 1103 5 0.1699073684502963 1103 6 0.13810382874706306 1103 7 0.14569506514727237 1103 8 0.1514148167427436 1103 9 0.22969298126007942 1103 10-11 0.24009454869913327 1103 12-13 0.23934339394306647 1103 14-15 0.2787776710020182 1103 16-17 0.28072788450588604 1103 18-19 0.15166091973785 1103 20-21 0.21867061675524013 1103 22-23 0.2805994695072158 1103 24-25 0.29107625484134303 1103 26-27 0.220460613010097 1103 28-29 0.30264206775189706 1103 30-31 0.34269121841767003 1103 32-33 0.3455398707747648 1103 34-35 0.3529001413310979 1103 36-37 0.3501044713712176 1103 38-39 0.30521033616697224 1103 40-41 0.4014512546201203 1103 42-43 0.33774025132604635 1103 44-45 0.35017222874599696 1103 46-47 0.3666776804798957 1103 48-49 0.5432919629730222 1103 50-51 0.3940283679622638 1103 52-53 0.41374743956930615 1103 54-55 0.47596879928347136 1103 56-57 0.48302643493551756 1103 58-59 0.43877391963621903 1103 60-61 0.4079297376187867 1103 62-63 0.3898114450502561 1103 64-65 0.36933693183582506 1103 66-67 0.39135522056572114 1103 68-69 0.48726293963830614 1103 70-71 0.30046963175050934 1103 72-73 0.35342195801052156 1103 74-75 0.16249317322087364 1103 76-77 0.28558603509403113 1103 78-79 0.44997176249602333 1103 80-81 0.391709148946628 1103 82-83 0.5308500222058328 1103 84-85 0.4671238773516784 1103 86-87 0.5112363529302364 1103 88-89 0.4541420069843838 1103 90-91 0.5855977950814015 1103 92-93 0.584110530682473 1103 94-95 0.4197185538182069 1103 96-97 0.09012321855407279 1103 98-99 -0.09519669087848115 1103 100-101 -0.31660014390893565 1104 1 -0.05862304611182623 1104 2 0.010010111082827677 1104 3 0.060032569434468996 1104 4 -0.030296927135530893 1104 5 0.024849001497806 1104 6 0.03607670843904742 1104 7 0.10303963725078091 1104 8 -0.08045768099466954 1104 9 0.04727132796438127 1104 10-11 0.0026461279048533015 1104 12-13 -0.01762267499282899 1104 14-15 -0.01867903314425945 1104 16-17 0.01463287139058167 1104 18-19 0.0777952787751417 1104 20-21 0.06892793755105942 1104 22-23 0.11087512105419961 1104 24-25 0.1596566570441027 1104 26-27 0.1073008210142703 1104 28-29 0.0799406979309083 1104 30-31 0.1012967007695309 1104 32-33 0.1740188786132606 1104 34-35 0.23426988562095374 1104 36-37 0.2704865026331973 1104 38-39 0.2510003992124794 1104 40-41 0.3983622546047769 1104 42-43 0.35026690377807057 1104 44-45 0.23090346465280476 1104 46-47 0.27649076817604623 1104 48-49 0.22691010044598414 1104 50-51 0.24014976508763652 1104 52-53 0.16867718447274171 1104 54-55 0.29879902124663005 1104 56-57 0.33897472534369655 1104 58-59 0.3938578269240338 1104 60-61 0.15428851413700784 1104 62-63 0.12236271746516536 1104 64-65 0.11411190077325983 1104 66-67 0.21454400429352916 1104 68-69 -0.03976341369267544 1104 70-71 -0.13547819847145348 1104 72-73 -0.21013742902143662 1104 74-75 -0.08069223580798024 1104 76-77 0.01664745461512851 1104 78-79 0.056662103187221646 1104 80-81 -0.14428124850087443 1104 82-83 -0.012650997873620895 1104 84-85 -0.06693757284850221 1104 86-87 -0.12640151119211396 1104 88-89 -0.1251001430056462 1104 90-91 0.017087571277500047 1104 92-93 0.041740801732156996 1104 94-95 -0.10131003908572112 1104 96-97 -0.10026732615301626 1104 98-99 -0.13450618145233761 1104 100-101 -0.49140188034494514 1105 1 0.0882456424354956 1105 2 0.07294430137586971 1105 3 0.07554384481465348 1105 4 0.04338994016121944 1105 5 0.08723062727348463 1105 6 0.10980590093144116 1105 7 0.11675715525976216 1105 8 0.0735395572213946 1105 9 0.061128545085367136 1105 10-11 0.16135574434510147 1105 12-13 0.10470999377434254 1105 14-15 0.2372009232529919 1105 16-17 0.1640776842690741 1105 18-19 0.17719703939941667 1105 20-21 0.07253531657239876 1105 22-23 0.12270436407717256 1105 24-25 0.009873761928012925 1105 26-27 0.08952852329624506 1105 28-29 0.06287221888032235 1105 30-31 0.07610178415535529 1105 32-33 0.018922353797456992 1105 34-35 0.20671150930893845 1105 36-37 0.28751538295779966 1105 38-39 0.2157442902326352 1105 40-41 0.25505225812062093 1105 42-43 0.21883327929240437 1105 44-45 0.24915445254364244 1105 46-47 0.19486013678279335 1105 48-49 0.21734850118716764 1105 50-51 0.1675170028934332 1105 52-53 0.2220352343354577 1105 54-55 0.21014149855331965 1105 56-57 0.21370616808238907 1105 58-59 0.12669067563821557 1105 60-61 0.2710219230498154 1105 62-63 0.2924867640446891 1105 64-65 0.34327907594815343 1105 66-67 0.21897244017760897 1105 68-69 0.09251372857243467 1105 70-71 0.21892403733752985 1105 72-73 0.2624725866259112 1105 74-75 0.39926068195598674 1105 76-77 0.41238881287479856 1105 78-79 0.5070193226451778 1105 80-81 0.3984669394354583 1105 82-83 0.4369551441094899 1105 84-85 0.4747705318292397 1105 86-87 0.41134204693907606 1105 88-89 0.5528488714924187 1105 90-91 0.47647304059641016 1105 92-93 0.5195721366065058 1105 94-95 0.15545861658816307 1105 96-97 0.23547079669599214 1105 98-99 0.440783850003335 1105 100-101 0.09375926959167202 1106 1 0.18739827199194536 1106 2 0.12928235795598653 1106 3 0.07874386216247586 1106 4 0.0413767860752543 1106 5 0.04928932947494502 1106 6 0.1125568416977103 1106 7 0.035636929818785745 1106 8 0.13638304659434652 1106 9 0.0986618665172827 1106 10-11 0.1610415672420089 1106 12-13 0.09026894156849607 1106 14-15 0.125720246405578 1106 16-17 0.08968087404918634 1106 18-19 0.09024058343816677 1106 20-21 0.13562881124046555 1106 22-23 0.17313369742664264 1106 24-25 0.2407765344526922 1106 26-27 0.26251403282292074 1106 28-29 0.31791695507745743 1106 30-31 0.30316685562938517 1106 32-33 0.26056110761876994 1106 34-35 0.1770705638123573 1106 36-37 0.2217976521737839 1106 38-39 0.27508455361692796 1106 40-41 0.2843038862438618 1106 42-43 0.2740978133874279 1106 44-45 0.32432094623639074 1106 46-47 0.3214504377372265 1106 48-49 0.303023033880244 1106 50-51 0.229376860742164 1106 52-53 0.30868570802174133 1106 54-55 0.23942855354894022 1106 56-57 0.17731961829161946 1106 58-59 0.13371931668859816 1106 60-61 0.1307128494335359 1106 62-63 0.2650875453017818 1106 64-65 0.3445187643328822 1106 66-67 0.28539177432386964 1106 68-69 0.11820148897886185 1106 70-71 0.20027449962669408 1106 72-73 0.08489557793485147 1106 74-75 0.0020885028175499087 1106 76-77 0.09603390268507539 1106 78-79 0.2893165271245479 1106 80-81 0.16566284071022253 1106 82-83 0.031180916919609558 1106 84-85 0.277144560931216 1106 86-87 0.3911056122793397 1106 88-89 0.3151609073499628 1106 90-91 0.3513158259705804 1106 92-93 0.5069306686581179 1106 94-95 0.32147953079814684 1106 96-97 0.4458403557083379 1106 98-99 0.4939783705125933 1106 100-101 0.3022905395429234 1107 1 -0.04944765953449348 1107 2 -0.09422800486643013 1107 3 -0.07224405298838832 1107 4 -0.023276860051723247 1107 5 -0.06367799652716855 1107 6 0.009310410872537034 1107 7 -0.07370929349276878 1107 8 -0.004268257338921444 1107 9 0.03227729176194316 1107 10-11 0.04017527208208094 1107 12-13 0.0035870031658191692 1107 14-15 0.11564723089052364 1107 16-17 -0.06839123742657449 1107 18-19 -0.020358337649291514 1107 20-21 0.0884943883122915 1107 22-23 0.051650944399490584 1107 24-25 0.0074542790625784505 1107 26-27 0.08648304227676107 1107 28-29 0.016931215951153433 1107 30-31 0.1013346429487001 1107 32-33 0.06414571244281575 1107 34-35 0.07072305071169893 1107 36-37 0.04960190491706129 1107 38-39 0.0234112401843376 1107 40-41 0.11648318473310582 1107 42-43 0.027847252072000117 1107 44-45 -0.026896836236318222 1107 46-47 0.03553985058645992 1107 48-49 0.0183490715966812 1107 50-51 -0.051735344975242015 1107 52-53 -0.08453058486325915 1107 54-55 -0.09111798469272259 1107 56-57 -0.049630346990902297 1107 58-59 -0.05394697609748533 1107 60-61 0.019743509619601696 1107 62-63 -0.2104054726266824 1107 64-65 -0.12361113048600458 1107 66-67 -0.09693302108036761 1107 68-69 -0.22339999586117187 1107 70-71 -0.08920427325370639 1107 72-73 -0.15805682251697561 1107 74-75 -0.029230672508710143 1107 76-77 -0.05556033203675881 1107 78-79 0.0680445022941285 1107 80-81 -0.09640000090090695 1107 82-83 -0.034792933199778275 1107 84-85 0.09899936470325699 1107 86-87 0.1923509906999037 1107 88-89 0.17661020894498947 1107 90-91 0.24953969537745735 1107 92-93 0.24498330471405438 1107 94-95 0.2307912344473415 1107 96-97 0.4319751228440367 1107 98-99 0.3321113216568854 1107 100-101 0.19991328216471516 1108 1 0.03610827724662613 1108 2 0.02744071818827365 1108 3 -0.01357439428509366 1108 4 0.045544920394846145 1108 5 0.07733160557847185 1108 6 0.09917377177198716 1108 7 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74-75 -0.20137296024795148 2316 76-77 -0.06929303764819039 2316 78-79 -0.1513759364446976 2316 80-81 -0.01325045945002401 2316 82-83 -0.1460241514362579 2316 84-85 -0.26838903878978115 2316 86-87 -0.3226796075360703 2316 88-89 -0.47042769846087396 2316 90-91 -0.4953146663755206 2316 92-93 -0.5163412116391441 2316 94-95 -0.24471753832967735 2316 96-97 -0.4198283829468217 2316 98-99 -0.165832384832445 2316 100-101 0.04141117531743532 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 8.0 4 17.0 5 44.0 6 72.0 7 195.0 8 455.0 9 821.0 10 1441.0 11 2363.0 12 3320.0 13 4844.0 14 6020.0 15 8195.0 16 10465.0 17 14868.0 18 14961.0 19 10854.0 20 10354.0 21 10909.0 22 11675.0 23 12507.0 24 14230.0 25 16847.0 26 19747.0 27 23743.0 28 29139.0 29 35424.0 30 43740.0 31 53261.0 32 67136.0 33 87098.0 34 121793.0 35 180358.0 36 299210.0 37 506402.0 38 440319.0 39 42564.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.11730219256435 18.69380362249762 14.626024785510008 23.562869399428028 2 15.979008324285196 19.962230562656323 39.547621693868074 24.511139419190407 3 19.965555337070377 23.36580213032546 29.04912401693545 27.619518515668712 4 13.751599453977049 16.389475474089082 35.47135326868072 34.387571803253145 5 14.714169143623604 36.4951938011006 33.40913818997381 15.38149886530199 6 33.47140646507135 34.73662561995169 16.807684598599984 14.984283316376985 7 29.73649737865286 31.732739433629426 19.887043164121316 18.6437200235964 8 26.461737071139723 35.24621854407178 19.084727601232256 19.207316783556237 9 26.365033632468037 17.17711453277895 19.920338405039217 36.53751342971379 10-11 25.770302734959433 26.019375835349575 26.31131477729236 21.899006652398633 12-13 26.494414853952158 23.173677665970363 27.111065514204864 23.220841965872616 14-15 23.161661766141275 24.78207683007372 25.33458820981326 26.721673193971746 16-17 23.02435041533188 27.89946823934763 26.051403450566397 23.02477789475409 18-19 23.014139569920918 26.58704139135325 27.979339827258766 22.419479211467067 20-21 23.60463967519804 25.68224370976429 28.11937460043336 22.59374201460431 22-23 23.283585208743588 25.55184130756728 28.07038167460338 23.094191809085753 24-25 23.25940825901047 25.765965809306795 28.066054034926896 22.908571896755838 26-27 23.022514911185972 25.926191559015997 28.13427013856152 22.91702339123651 28-29 22.808379951420296 26.35011964438213 28.07000170038463 22.771498703812945 30-31 23.025599251425547 25.84968662944377 27.881786034051586 23.242928085079097 32-33 22.890786860111543 25.974871354978564 27.972229584222713 23.162112200687183 34-35 23.45717659034916 25.999863669258772 27.718224783741206 22.82473495665086 36-37 23.388763066988403 25.753779289224244 27.83891502722076 23.018542616566588 38-39 23.060553133075512 26.06510932752603 27.919535814975895 22.954801724422566 40-41 23.304597735275433 25.989228604171988 27.756830098153902 22.94934356239868 42-43 23.09204879209682 26.056621367229454 27.472986831986102 23.37834300868763 44-45 23.22095412526801 26.076499685086794 27.533792609466772 23.16875358017842 46-47 23.415971484530015 26.390678942814933 27.158852532092137 23.03449704056292 48-49 23.449082985278512 26.323014033256996 27.12429018545957 23.103612796004924 50-51 23.18504040790252 26.59994157893612 27.127868509859148 23.087149503302214 52-53 23.5471085135639 26.51235747991479 26.797148292133816 23.14338571438749 54-55 23.16332336850945 26.3162544421361 26.952141297212982 23.56828089214147 56-57 23.26050177495457 26.550437302829007 26.918893553072426 23.270167369144005 58-59 23.49066226824886 26.260050515305995 26.96344112069406 23.285846095751086 60-61 23.277950922034734 26.36687771740292 27.237634198674606 23.117537161887743 62-63 23.077536908398677 26.45603612003628 27.272042857275174 23.194384114289868 64-65 22.930571625619134 26.420870690293935 27.131484151909124 23.51707353217781 66-67 23.013899556314268 26.434877564872593 26.92128841282485 23.62993446598829 68-69 22.964364519316792 26.31046464654141 27.193983465311756 23.53118736883004 70-71 23.10771012994884 26.300919229098625 26.997839608569468 23.593531032383073 72-73 23.06958847842174 26.30870245453846 27.060077723726444 23.561631343313355 74-75 22.93586130181067 26.699244706246684 26.68516191176429 23.679732080178358 76-77 23.059340625864223 26.377630992098037 26.822565497833995 23.74046288420374 78-79 23.04096969812114 26.428548528247042 26.875756240804883 23.654725532826937 80-81 23.083958334322887 26.530995140904277 26.803992723231033 23.581053801541803 82-83 23.08838449086916 26.496633406035553 26.82194099721097 23.593041105884318 84-85 23.03156730815814 26.520039365329062 26.725961643502487 23.72243168301031 86-87 22.999626749463303 26.452360631340206 26.813129860280675 23.734882758915816 88-89 22.863948725040892 26.453148173758777 26.651604965354114 24.031298135846214 90-91 22.827258283854945 26.406748578589102 26.762339715775042 24.003653421780914 92-93 22.901853609232614 26.270728781052206 26.830003334273773 23.99741427544141 94-95 22.751597554105953 26.14716591479268 26.943781864400503 24.157454666700865 96-97 22.737443514457546 25.983325781345734 27.06561109828698 24.213619605909738 98-99 22.576350467031148 26.1807940491319 26.72772788296233 24.51512760087462 100-101 22.707663970175602 25.407899389685667 27.349890673487888 24.534545966650843 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 317.0 1 224.5 2 189.5 3 269.0 4 247.0 5 226.5 6 385.5 7 825.5 8 1395.0 9 1740.5 10 1654.5 11 1504.0 12 1574.5 13 1715.0 14 1837.0 15 1969.0 16 2076.0 17 2143.5 18 2341.5 19 2552.5 20 2806.5 21 3246.0 22 3916.0 23 4812.0 24 5882.5 25 7338.5 26 9323.0 27 11814.5 28 14144.5 29 16689.0 30 19601.0 31 22775.0 32 26609.0 33 29550.5 34 31908.5 35 34417.5 36 37885.0 37 42231.0 38 45842.5 39 48639.5 40 51646.0 41 56731.5 42 61560.5 43 65019.5 44 68928.0 45 73454.0 46 76768.0 47 81549.0 48 89534.0 49 90564.0 50 85519.0 51 82637.0 52 81541.5 53 81922.5 54 81983.0 55 77525.5 56 70466.5 57 64389.5 58 58946.0 59 52160.5 60 44162.0 61 36732.5 62 30098.0 63 23696.5 64 17962.0 65 13534.5 66 10021.0 67 8088.5 68 6321.0 69 4437.0 70 3271.0 71 2441.0 72 1912.5 73 1585.0 74 1268.5 75 950.0 76 670.0 77 376.5 78 207.5 79 136.5 80 83.0 81 51.0 82 31.0 83 18.5 84 14.0 85 11.0 86 6.0 87 4.0 88 3.0 89 2.5 90 1.0 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35176113300712547 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.261871582013161E-4 16-17 0.001971116259761775 18-19 1.1874194335914307E-4 20-21 0.0 22-23 0.0 24-25 4.037226074210865E-4 26-27 0.001116174267575945 28-29 0.0 30-31 0.003372271191399663 32-33 0.0 34-35 0.010995503955056649 36-37 0.02750063408197754 38-39 0.02229973696284707 40-41 0.0139403041503634 42-43 0.010876762011697506 44-45 0.003039793749994063 46-47 0.0010686774902322876 48-49 6.887032714830299E-4 50-51 4.749677734365723E-5 52-53 4.749677734365723E-5 54-55 0.0 56-57 0.0 58-59 0.004915916455068523 60-61 0.010686774902322877 62-63 0.004393451904288294 64-65 0.001994864648433604 66-67 0.0041322196288981795 68-69 0.0035385099121024643 70-71 2.3748388671828615E-5 72-73 0.0 74-75 0.0 76-77 0.21539788525348555 78-79 4.037226074210865E-4 80-81 0.0036097550781179497 82-83 6.649548828112012E-4 84-85 0.0 86-87 0.107390213574009 88-89 0.4025826847648387 90-91 0.3891885935539274 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0015436452636688601 100-101 7.362000488266872E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2105406.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.156227453708574 #Duplication Level Percentage of deduplicated Percentage of total 1 78.06233781203505 46.17873411176981 2 12.761156141626321 15.098037105726736 3 3.872201814759592 6.871945539017446 4 1.767154492738108 4.1815277247303415 5 0.9498887874504539 2.8095918583073245 6 0.5669182675378356 2.012204758927837 7 0.39435565636449776 1.6330015033888339 8 0.2675532939279973 1.2661954809274847 9 0.2112011820234024 1.1244478646041647 >10 1.0533899933690614 11.128043658900118 >50 0.06241655701283092 2.520509291035428 >100 0.0286145486298823 3.189200600861714 >500 0.002088507589973535 0.8693993910200475 >1k 6.426177199918417E-4 0.8551438260164244 >5k 8.032721499898021E-5 0.2620172847663127 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5514 0.261897230272926 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4905 0.23297169287063874 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3670 0.17431317285122203 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2984 0.14173038359347317 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.749677734365723E-5 0.0 2 0.0 0.0 0.0 4.749677734365723E-5 0.0 3 0.0 0.0 0.0 4.749677734365723E-5 0.0 4 0.0 0.0 0.0 4.749677734365723E-5 0.0 5 0.0 0.0 0.0 4.749677734365723E-5 0.0 6 0.0 0.0 0.0 4.749677734365723E-5 0.0 7 0.0 0.0 0.0 4.749677734365723E-5 0.0 8 0.0 0.0 0.0 4.749677734365723E-5 0.0 9 0.0 0.0 0.0 4.749677734365723E-5 0.0 10-11 0.0 0.0 0.0 7.124516601548585E-5 0.0 12-13 0.0 0.0 0.0 9.499355468731446E-5 9.499355468731446E-5 14-15 0.0 0.0 0.0 9.499355468731446E-5 1.1874194335914309E-4 16-17 0.0 0.0 0.0 9.499355468731446E-5 1.424903320309717E-4 18-19 0.0 0.0 0.0 1.662387207028003E-4 1.424903320309717E-4 20-21 0.0 0.0 0.0 2.3748388671828618E-4 1.424903320309717E-4 22-23 0.0 0.0 0.0 3.7997421874925785E-4 1.424903320309717E-4 24-25 0.0 0.0 0.0 4.749677734365723E-4 1.424903320309717E-4 26-27 0.0 0.0 0.0 7.362000488266871E-4 1.424903320309717E-4 28-29 0.0 0.0 0.0 0.002469832421870176 1.424903320309717E-4 30-31 0.0 0.0 0.0 0.007765723095687957 1.424903320309717E-4 32-33 0.0 0.0 0.0 0.016528878515592718 1.662387207028003E-4 34-35 0.0 0.0 0.0 0.029566743896426627 2.3748388671828618E-4 36-37 0.0 0.0 0.0 0.052507687353413074 2.3748388671828618E-4 38-39 0.0 0.0 0.0 0.08694285092756457 2.3748388671828618E-4 40-41 0.0 0.0 0.0 0.12301665332007222 2.3748388671828618E-4 42-43 0.0 0.0 0.0 0.16153653974577825 2.3748388671828618E-4 44-45 0.0 0.0 0.0 0.2095320332515439 2.612322753901148E-4 46-47 0.0 0.0 0.0 0.2719190502924377 2.849806640619434E-4 48-49 0.0 0.0 0.0 0.3364196739251242 3.08729052733772E-4 50-51 0.0 0.0 0.0 0.4048387816886624 3.7997421874925785E-4 52-53 0.0 0.0 0.0 0.47539524443266523 3.7997421874925785E-4 54-55 0.0 0.0 0.0 0.5573746821278176 4.2747099609291507E-4 56-57 0.0 0.0 0.0 0.6595402501940244 4.2747099609291507E-4 58-59 0.0 0.0 0.0 0.7732712835434116 4.2747099609291507E-4 60-61 0.0 0.0 0.0 0.898900259617385 4.2747099609291507E-4 62-63 0.0 0.0 0.0 1.027735268162055 4.2747099609291507E-4 64-65 0.0 0.0 0.0 1.1655946644020203 4.2747099609291507E-4 66-67 0.0 0.0 0.0 1.327463681589204 4.2747099609291507E-4 68-69 0.0 0.0 0.0 1.5150522037079783 4.2747099609291507E-4 70-71 0.0 0.0 0.0 1.7192645978970327 4.2747099609291507E-4 72-73 0.0 0.0 0.0 1.934543741207159 4.749677734365723E-4 74-75 0.0 0.0 0.0 2.1559499687946175 5.224645507802295E-4 76-77 0.0 0.0 0.0 2.4039068949171796 5.224645507802295E-4 78-79 0.0 0.0 0.0 2.692663552777944 5.224645507802295E-4 80-81 0.0 0.0 0.0 3.007614683343735 5.224645507802295E-4 82-83 0.0 0.0 0.0 3.3312814725520874 6.174581054675441E-4 84-85 0.0 0.0 0.0 3.662903971965502 6.174581054675441E-4 86-87 0.0 0.0 0.0 4.016944950285123 6.649548828112012E-4 88-89 0.0 0.0 0.0 4.408674621426936 7.124516601548585E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7300 0.0 26.096554 1 GTATCAA 9630 0.0 24.381851 1 GTACATG 7180 0.0 23.813107 1 TACATGG 7265 0.0 22.729406 2 ACATGGG 7445 0.0 21.478783 3 GAGTACT 5935 0.0 21.387392 12-13 ATACCGG 115 0.0037466737 20.630827 6 ATCAACG 11250 0.0 20.583147 3 TCAACGC 11330 0.0 20.521574 4 CAACGCA 11340 0.0 20.461632 5 GTACTTT 6210 0.0 20.28746 14-15 TATCAAC 11560 0.0 20.195366 2 AACGCAG 11635 0.0 19.98362 6 AGAGTAC 9415 0.0 19.152172 10-11 CATGGGG 4205 0.0 18.506416 4 AGTACTT 6320 0.0 18.320086 12-13 ACGCAGA 13020 0.0 17.857864 7 CGCAGAG 13100 0.0 17.603922 8 GTCTAGC 515 0.0 17.570837 1 TAAGGTA 650 0.0 17.520332 4 >>END_MODULE