FastQCFastQC Report
Fri 27 May 2016
SRR939084_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939084_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2070816
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50670.24468615270502062No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42450.20499165546335357No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31680.1529831718510964No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25620.12371934541745863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA98900.026.9854491
ATCAACG112750.023.5264843
TCAACGC113250.023.4226174
CAACGCA113600.023.308195
TATCAAC115850.023.0599382
GTACATG74350.022.929951
AACGCAG116850.022.729446
GGTATCA71550.022.5662251
TACATGG74950.021.929712
GAGTACT52150.020.69882212-13
ACATGGG77500.020.5974663
ACGCAGA129250.020.4755637
AGAGTAC90900.020.20795610-11
CGCAGAG130700.020.1397388
GTACTTT54900.019.44687314-15
GCAGAGT139400.018.3394189
AGTACTT54950.018.3086512-13
CATGGGG46450.017.74394
GAGTACA60300.017.0896071
TACCTGG16950.016.7670482
Produced by FastQC (version 0.11.5)