##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939084_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2070816 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.060463121783876 33.0 31.0 34.0 30.0 34.0 2 32.163271386738366 34.0 31.0 34.0 30.0 34.0 3 32.2281921715884 34.0 31.0 34.0 30.0 34.0 4 35.657350532350534 37.0 35.0 37.0 35.0 37.0 5 35.68870677066432 37.0 35.0 37.0 35.0 37.0 6 35.768649653083614 37.0 36.0 37.0 35.0 37.0 7 35.692079354225584 37.0 36.0 37.0 35.0 37.0 8 35.6838144963145 37.0 36.0 37.0 35.0 37.0 9 37.36265124472671 39.0 38.0 39.0 35.0 39.0 10-11 37.26145538763463 39.0 37.0 39.0 34.0 39.0 12-13 37.156557849659265 39.0 37.0 39.0 34.0 39.0 14-15 38.45798250544713 40.0 38.0 41.0 34.0 41.0 16-17 38.355355328527494 40.0 38.0 41.0 33.5 41.0 18-19 38.22540631326009 40.0 38.0 41.0 33.5 41.0 20-21 38.19901575031292 40.0 38.0 41.0 33.5 41.0 22-23 38.24083911849242 40.0 38.0 41.0 34.0 41.0 24-25 38.184901265974375 40.0 38.0 41.0 33.5 41.0 26-27 38.06719211170862 40.0 38.0 41.0 33.0 41.0 28-29 37.92343597886051 40.0 38.0 41.0 33.0 41.0 30-31 37.71967765363992 40.0 38.0 41.0 32.5 41.0 32-33 37.5237744444702 40.0 37.5 41.0 32.0 41.0 34-35 37.209601673929505 40.0 37.0 41.0 31.0 41.0 36-37 36.951019066879915 40.0 37.0 41.0 30.5 41.0 38-39 36.76053014850184 40.0 36.5 41.0 30.0 41.0 40-41 36.57234104816652 40.0 36.0 41.0 30.0 41.0 42-43 36.364013268199585 40.0 36.0 41.0 30.0 41.0 44-45 36.09414259885958 39.0 35.5 41.0 29.0 41.0 46-47 35.98337708420256 39.0 35.5 41.0 28.0 41.0 48-49 35.86842167532026 39.5 35.0 41.0 27.5 41.0 50-51 34.74095549773616 38.0 34.0 40.0 25.5 40.5 52-53 34.87940478535998 38.5 34.5 39.5 26.0 40.5 54-55 35.395092079643966 39.0 35.0 40.5 27.0 41.0 56-57 35.283914408619594 39.0 35.0 41.0 26.0 41.0 58-59 35.0624118704897 39.0 35.0 41.0 26.0 41.0 60-61 34.74466949260581 38.0 35.0 40.0 26.0 41.0 62-63 34.348999621405284 37.5 34.0 40.0 24.5 41.0 64-65 33.95834419861542 37.0 34.0 40.0 23.5 41.0 66-67 33.56067197665075 36.5 34.0 39.5 22.5 41.0 68-69 33.137393182204505 36.0 33.0 39.0 22.0 41.0 70-71 32.702243704897 35.5 33.0 39.0 20.5 40.5 72-73 32.25240484910296 35.0 32.5 37.5 19.5 40.0 74-75 31.807453197193766 35.0 32.0 37.0 18.0 39.0 76-77 31.36504933320971 35.0 31.5 36.5 14.5 39.0 78-79 30.93969382117967 35.0 31.0 36.0 9.5 38.5 80-81 30.50481066400878 35.0 31.0 36.0 7.0 37.0 82-83 30.108348110116978 34.5 31.0 35.0 2.0 37.0 84-85 29.730975615409577 34.0 30.0 35.0 2.0 36.5 86-87 29.323517637491694 34.0 30.0 35.0 2.0 36.0 88-89 28.90558045717244 34.0 29.0 35.0 2.0 36.0 90-91 28.496132683927495 34.0 29.0 35.0 2.0 35.0 92-93 28.08195271815555 34.0 29.0 35.0 2.0 35.0 94-95 27.587458518767484 34.0 27.0 35.0 2.0 35.0 96-97 26.973007741875666 34.0 25.5 35.0 2.0 35.0 98-99 26.237722955588524 33.0 24.5 35.0 2.0 35.0 100-101 23.295333095745832 30.0 10.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31670274780000085 1101 2 0.5156108621110675 1101 3 0.4327946218653196 1101 4 0.42502262239285926 1101 5 0.24138952756326404 1101 6 0.2757839337131216 1101 7 0.3844851693186371 1101 8 0.3703675301298617 1101 9 0.4638220453002546 1101 10-11 0.42181499481147 1101 12-13 0.38159151192825647 1101 14-15 0.5071339903168948 1101 16-17 0.5791361966219313 1101 18-19 0.5952504258549922 1101 20-21 0.5363171345931264 1101 22-23 0.5146609820853598 1101 24-25 0.6278493757279549 1101 26-27 0.6395241618833865 1101 28-29 0.66135510836191 1101 30-31 0.6428243681891601 1101 32-33 0.539530173286991 1101 34-35 0.6357275337402584 1101 36-37 0.617962348103795 1101 38-39 0.46545055566239313 1101 40-41 0.6491457873994619 1101 42-43 0.43718828415897093 1101 44-45 0.505730890182484 1101 46-47 0.6515635556331532 1101 48-49 0.5992945346531755 1101 50-51 0.6229528924077599 1101 52-53 0.7341348416762514 1101 54-55 0.7403601001355966 1101 56-57 0.6190731240548644 1101 58-59 0.7498224828237952 1101 60-61 0.5688698551470992 1101 62-63 0.484737801945343 1101 64-65 0.4079778998172401 1101 66-67 0.39928716329293223 1101 68-69 0.47415600550443315 1101 70-71 0.36906857814313554 1101 72-73 0.2455835990996249 1101 74-75 0.13414850544678458 1101 76-77 0.10353439761448868 1101 78-79 0.05239791208476419 1101 80-81 0.02397404111880519 1101 82-83 -0.27385211706719303 1101 84-85 -0.2435151146768817 1101 86-87 -0.2876397416750329 1101 88-89 -0.34816862248045766 1101 90-91 -0.3360021926968564 1101 92-93 -0.5243247284969144 1101 94-95 -0.6359019747963401 1101 96-97 -0.49256218292135046 1101 98-99 -0.7526383015637848 1101 100-101 -0.8146487629092292 1102 1 0.20486772088359828 1102 2 0.26298594070222947 1102 3 0.25150852014570546 1102 4 0.17407275537890854 1102 5 0.18661222820934142 1102 6 0.2465073834470104 1102 7 0.14187631958805014 1102 8 0.22862224116195762 1102 9 0.29880598763528354 1102 10-11 0.29855369286540423 1102 12-13 0.302497274470376 1102 14-15 0.23783201684185684 1102 16-17 0.3041421834077127 1102 18-19 0.3342488311434195 1102 20-21 0.4121194339548424 1102 22-23 0.4518752473580463 1102 24-25 0.4467342360575586 1102 26-27 0.4292849053222909 1102 28-29 0.4682031816445331 1102 30-31 0.4210777152617382 1102 32-33 0.39172538516626787 1102 34-35 0.4972467168343826 1102 36-37 0.5052082055438589 1102 38-39 0.42082992792337137 1102 40-41 0.363819780833154 1102 42-43 0.38824253455889135 1102 44-45 0.34264726420509106 1102 46-47 0.5327930436905675 1102 48-49 0.5379179133552938 1102 50-51 0.22770451631367195 1102 52-53 0.3361546539667728 1102 54-55 0.36896497819045493 1102 56-57 0.43229101285106 1102 58-59 0.29036929357192065 1102 60-61 0.30949255560633304 1102 62-63 0.21688713745105304 1102 64-65 0.17424194930094217 1102 66-67 0.2607633723200351 1102 68-69 0.2059266709703209 1102 70-71 0.10084905290693058 1102 72-73 0.10880508463838368 1102 74-75 -0.013687579005353001 1102 76-77 -0.07196281701201457 1102 78-79 -0.08986135295281272 1102 80-81 -0.09869823584345028 1102 82-83 -0.3472722206471488 1102 84-85 -0.32892903123681094 1102 86-87 -0.4620478128276311 1102 88-89 -0.5301826363859945 1102 90-91 -0.250964454926482 1102 92-93 -0.3774899709469466 1102 94-95 -0.4819669982966168 1102 96-97 -0.6841429092391351 1102 98-99 -0.585171165047317 1102 100-101 -0.5349631026391606 1103 1 0.11460489947216956 1103 2 0.23167148167109275 1103 3 0.30530046346089534 1103 4 0.18156707069542222 1103 5 0.16977361010780356 1103 6 0.16844106790475166 1103 7 0.1818951562654405 1103 8 0.3401785791516687 1103 9 0.20258479261074314 1103 10-11 0.21167179409918901 1103 12-13 0.30963294042035017 1103 14-15 0.5691854964385925 1103 16-17 0.516201662158494 1103 18-19 0.4823587307762196 1103 20-21 0.482821300448677 1103 22-23 0.5262302094935336 1103 24-25 0.6116114487147897 1103 26-27 0.5572942071454534 1103 28-29 0.5304162545656581 1103 30-31 0.615997729170779 1103 32-33 0.5868614382876416 1103 34-35 0.6686636670525203 1103 36-37 0.6011901492777909 1103 38-39 0.43839965063461506 1103 40-41 0.4472183507263878 1103 42-43 0.46568288255194545 1103 44-45 0.6099631711844395 1103 46-47 0.6635189172384415 1103 48-49 0.7784762108692078 1103 50-51 0.6057619111356516 1103 52-53 0.5041180110462165 1103 54-55 0.5757384750339156 1103 56-57 0.5845349381136273 1103 58-59 0.6014606010885686 1103 60-61 0.6531529880852744 1103 62-63 0.4561173399756768 1103 64-65 0.46991330632454975 1103 66-67 0.34931001706721787 1103 68-69 0.19357359735738555 1103 70-71 0.2882859782562761 1103 72-73 0.40159905423146824 1103 74-75 0.1985384805425774 1103 76-77 0.1739368376286734 1103 78-79 0.07003284851831992 1103 80-81 0.026930538394964287 1103 82-83 -0.12017101173784184 1103 84-85 -0.14861184197978616 1103 86-87 -0.06568397730629627 1103 88-89 0.0014921408539123604 1103 90-91 -0.07587665865388615 1103 92-93 -0.24433471825085462 1103 94-95 -0.3998499475231547 1103 96-97 -0.20816832054806156 1103 98-99 -0.1778233133934357 1103 100-101 -0.48561543789560346 1104 1 0.21596963108170542 1104 2 0.2937135459505882 1104 3 0.3164248484205032 1104 4 0.2513474351059486 1104 5 0.3127629450687124 1104 6 0.26147220102538427 1104 7 0.42030086742714445 1104 8 0.2685003758072355 1104 9 0.36428263675133365 1104 10-11 0.293377072237746 1104 12-13 0.2989936455220388 1104 14-15 0.4151608687893358 1104 16-17 0.41881281778360346 1104 18-19 0.34167234424882054 1104 20-21 0.44995319461399674 1104 22-23 0.4052740006589346 1104 24-25 0.5048009520253771 1104 26-27 0.5097950098436712 1104 28-29 0.46229870483452373 1104 30-31 0.49985799893523364 1104 32-33 0.4443943633778247 1104 34-35 0.40722340949486124 1104 36-37 0.4803134871139463 1104 38-39 0.41144735838882696 1104 40-41 0.37420564350730956 1104 42-43 0.49800312877058417 1104 44-45 0.5114070155737949 1104 46-47 0.5330383685689029 1104 48-49 0.5030721860969223 1104 50-51 0.3769258090960719 1104 52-53 0.459137550601163 1104 54-55 0.5079511163015695 1104 56-57 0.4819139476274117 1104 58-59 0.33276872439459027 1104 60-61 0.430785042790248 1104 62-63 0.44520946659083194 1104 64-65 0.3576169819592607 1104 66-67 0.2617343392572664 1104 68-69 0.20663371439223255 1104 70-71 0.2153779833819769 1104 72-73 0.10534396686030334 1104 74-75 0.012831715604438187 1104 76-77 -0.09180313442875487 1104 78-79 0.008965014010104966 1104 80-81 -0.06532696719499498 1104 82-83 -0.11691422640682347 1104 84-85 0.09261734210277695 1104 86-87 -0.012589756444238986 1104 88-89 -0.12173678867930704 1104 90-91 -0.10233344745602935 1104 92-93 -0.17963073310728106 1104 94-95 -0.4427522150371601 1104 96-97 -0.32770888790384234 1104 98-99 -0.500438431889048 1104 100-101 -0.48046717736434985 1105 1 0.1477496794214943 1105 2 0.23223172384326318 1105 3 0.2271409166345677 1105 4 0.34095942696333026 1105 5 0.18850079983623402 1105 6 0.2634136437045953 1105 7 0.2438059509562862 1105 8 0.2575128869528669 1105 9 0.2859121700087712 1105 10-11 0.24342922537731226 1105 12-13 0.23326190332678465 1105 14-15 0.20860106932656208 1105 16-17 0.297437180590002 1105 18-19 0.37407780967352977 1105 20-21 0.38215523475508917 1105 22-23 0.30051054038224834 1105 24-25 0.2798731532804126 1105 26-27 0.3614325062686632 1105 28-29 0.31014626625465525 1105 30-31 0.37550052000678136 1105 32-33 0.3949188389022993 1105 34-35 0.3855027484397979 1105 36-37 0.4172480040805411 1105 38-39 0.5179482752746694 1105 40-41 0.3654915829087315 1105 42-43 0.3027893008983753 1105 44-45 0.41012127244763263 1105 46-47 0.3179105933769719 1105 48-49 0.2784252011652555 1105 50-51 0.30384930660898846 1105 52-53 0.423067626139094 1105 54-55 0.38998015382379236 1105 56-57 0.38397890352155173 1105 58-59 0.4780505476234893 1105 60-61 0.39058730948907083 1105 62-63 0.32977466081921847 1105 64-65 0.4049404884447583 1105 66-67 0.4150185850836152 1105 68-69 0.34342915631999205 1105 70-71 0.4008338466846695 1105 72-73 0.5040109774759856 1105 74-75 0.5168413386998658 1105 76-77 0.39496188483425954 1105 78-79 0.26541644149200394 1105 80-81 0.3531802473226442 1105 82-83 0.4272170032296181 1105 84-85 0.257339808890201 1105 86-87 0.21642014704441337 1105 88-89 0.30295113366298665 1105 90-91 0.30315454891007576 1105 92-93 0.10030615037811685 1105 94-95 0.1970906806397359 1105 96-97 0.2449690356161014 1105 98-99 0.3355507393246633 1105 100-101 0.21336333053993428 1106 1 0.21263133775181586 1106 2 0.08568077762555504 1106 3 0.24450328106159702 1106 4 0.2556526581979739 1106 5 0.19882346780921267 1106 6 0.16092606227486783 1106 7 0.1626748727321825 1106 8 0.13054804568711376 1106 9 0.1556600601409528 1106 10-11 0.24106797819189296 1106 12-13 0.23807248790198088 1106 14-15 0.33623195847900433 1106 16-17 0.3373055179625979 1106 18-19 0.23462564480304593 1106 20-21 0.24105943558356557 1106 22-23 0.27627176866049297 1106 24-25 0.3574242241135366 1106 26-27 0.33203992045591235 1106 28-29 0.33167948700441485 1106 30-31 0.33616018672501724 1106 32-33 0.4310422683184072 1106 34-35 0.44838733738082936 1106 36-37 0.42143927154818783 1106 38-39 0.5876217184414472 1106 40-41 0.7322615944249122 1106 42-43 0.4391980566349787 1106 44-45 0.46926793619228135 1106 46-47 0.46587044572918757 1106 48-49 0.6458811345776567 1106 50-51 0.7140961310645437 1106 52-53 0.5625219395511536 1106 54-55 0.6431363613900984 1106 56-57 0.6810045699991463 1106 58-59 0.6632693336115594 1106 60-61 0.8131898593782054 1106 62-63 0.6413534736210877 1106 64-65 0.6151296245591666 1106 66-67 0.6011757466023724 1106 68-69 0.7001230450083469 1106 70-71 0.6137698931962987 1106 72-73 0.6793910544797157 1106 74-75 0.7416829911861633 1106 76-77 0.9423963113455471 1106 78-79 0.8242311555640924 1106 80-81 0.7790544888874678 1106 82-83 0.915837789939598 1106 84-85 0.9794028599911151 1106 86-87 0.9082725551298019 1106 88-89 0.7247565746296587 1106 90-91 0.6812548943955612 1106 92-93 0.5653364877850855 1106 94-95 0.7826665542367373 1106 96-97 0.7491312977725499 1106 98-99 0.8237772686565492 1106 100-101 0.6707282087606004 1107 1 0.09243197187348784 1107 2 0.16539705752735046 1107 3 0.2402547275745448 1107 4 0.13914436773570316 1107 5 0.18460584934319968 1107 6 0.08024055701314126 1107 7 0.12581346656121895 1107 8 0.14446494984098734 1107 9 0.13850932053795617 1107 10-11 0.26323602043378 1107 12-13 0.24804849244199545 1107 14-15 0.20577977668101255 1107 16-17 0.2991055475713864 1107 18-19 0.26122035593224524 1107 20-21 0.22221390095978677 1107 22-23 0.27910542275575523 1107 24-25 0.31686595955896024 1107 26-27 0.29369768906319393 1107 28-29 0.3612609081031408 1107 30-31 0.3701405184668758 1107 32-33 0.2813112148621997 1107 34-35 0.356693375107362 1107 36-37 0.355815312482342 1107 38-39 0.38149400892839225 1107 40-41 0.45319948381138886 1107 42-43 0.4887033430383809 1107 44-45 0.43331599804998433 1107 46-47 0.5973492970313643 1107 48-49 0.5643517015860553 1107 50-51 0.49019350698519304 1107 52-53 0.4712428088159939 1107 54-55 0.402199428120916 1107 56-57 0.3526079632902892 1107 58-59 0.45866909859175564 1107 60-61 0.35975547061473634 1107 62-63 0.35260566343812627 1107 64-65 0.2790216233994869 1107 66-67 0.29857001853447684 1107 68-69 0.3997229407607179 1107 70-71 0.4078549968887657 1107 72-73 0.48760240575104774 1107 74-75 0.4426627029938963 1107 76-77 0.45406479771933306 1107 78-79 0.5073002458339033 1107 80-81 0.5958508482395288 1107 82-83 0.4169508184257289 1107 84-85 0.27126113564898446 1107 86-87 0.34143725712963047 1107 88-89 0.5127294671620746 1107 90-91 0.3215311814528157 1107 92-93 0.2601361349469613 1107 94-95 0.2929305505603139 1107 96-97 0.4218835739485769 1107 98-99 0.3400933332777427 1107 100-101 0.08383757932699254 1108 1 0.08427706823717784 1108 2 0.10648763524323357 1108 3 0.08083244345141338 1108 4 0.18003292279220062 1108 5 0.07345993586728383 1108 6 0.25787402323744146 1108 7 0.12134220740151846 1108 8 0.24964090620490254 1108 9 0.29230021036008935 1108 10-11 0.3224604379080773 1108 12-13 0.307835998663343 1108 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66-67 -1.4497299583779792 2316 68-69 -1.4636993417728519 2316 70-71 -1.4265549392683354 2316 72-73 -1.6781543177908347 2316 74-75 -1.392867054519062 2316 76-77 -1.2932182725553218 2316 78-79 -1.0777498707148965 2316 80-81 -1.0978588497730968 2316 82-83 -1.090825221822687 2316 84-85 -0.9998419368779281 2316 86-87 -1.035024781557265 2316 88-89 -1.208395186383484 2316 90-91 -1.3481682209450518 2316 92-93 -1.3059147759189145 2316 94-95 -1.087934128920132 2316 96-97 -1.1721503477970714 2316 98-99 -1.3742071234639894 2316 100-101 -1.2496627903374034 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8067.0 3 2339.0 4 2295.0 5 2498.0 6 2809.0 7 3295.0 8 3965.0 9 4889.0 10 5715.0 11 6960.0 12 8599.0 13 12875.0 14 10795.0 15 10799.0 16 12294.0 17 16493.0 18 14614.0 19 11476.0 20 11695.0 21 12189.0 22 13222.0 23 14558.0 24 16244.0 25 18410.0 26 20926.0 27 24451.0 28 28483.0 29 33925.0 30 40747.0 31 50037.0 32 62727.0 33 82084.0 34 113986.0 35 171775.0 36 281449.0 37 450559.0 38 414513.0 39 68031.0 40 28.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.84428480981362 18.142775283284678 14.23535194976066 23.77758795714104 2 15.570782194018614 19.53597160387169 40.33525570583978 24.557990496269912 3 19.922617188595343 23.221998648242277 28.89092050059514 27.964463662567233 4 13.389979334347437 15.87701374067652 35.85539071815319 34.87761620682286 5 14.731087077509414 36.347354581908334 33.44723693900218 15.47432140158008 6 33.385475789035205 34.37763219127341 17.18946848039294 15.04742353929845 7 29.728366039858724 31.583996582102046 20.082953620593997 18.604683757445237 8 26.399481532995967 34.965157644802744 19.391961151704276 19.243399670497013 9 26.44212994622505 17.335184631199137 20.07409702108904 36.148588401486776 10-11 25.73179678159231 25.890262137170396 26.485316703572646 21.89262437766465 12-13 26.5138360016838 23.086334311360662 27.176505499168492 23.223324187787046 14-15 23.241586962767364 24.66537168721293 25.470171052787364 26.62287029723234 16-17 23.118526920231215 27.805337507484623 26.02905990258273 23.04707566970143 18-19 23.059555325712257 26.52463639582077 27.981179700271987 22.43462857819499 20-21 23.66826084613546 25.657783290669638 28.051420685282103 22.6225351779128 22-23 23.380596277272822 25.5006497456482 27.968402326565524 23.150351650513453 24-25 23.32899065316649 25.655233090739454 28.04134650478702 22.974429751307035 26-27 23.029246384175043 25.910231678196716 28.048509593897847 23.012012343730397 28-29 22.89711595980592 26.298748617799717 27.975733699100285 22.82840172329408 30-31 23.030317091361155 25.842634174022027 27.82540375339734 23.30164498121948 32-33 22.922732078663564 25.92374105066006 27.881775065704012 23.27175180497236 34-35 23.531857264338132 26.007757478262345 27.614805590617376 22.845579666782147 36-37 23.43214039073755 25.646384579253795 27.769407315436162 23.152067714572492 38-39 23.117476732613078 25.993873995350526 27.847037726548958 23.04161154548744 40-41 23.338752648497202 25.931823381599283 27.70704739842545 23.022376571478066 42-43 23.162794806498404 25.979860971732023 27.408585989369627 23.44875823239995 44-45 23.283703664689426 26.008671256094285 27.50603208845332 23.201592990762972 46-47 23.46339237606124 26.347198423369854 27.112626147401325 23.076783053167578 48-49 23.48146337591638 26.260652335959062 27.061793925101053 23.1960903630235 50-51 23.211146367801692 26.5092987936969 27.143069697808926 23.136485140692482 52-53 23.505091382259373 26.448003289319587 26.83023620625335 23.216669122167687 54-55 23.203849469547524 26.28671616520915 26.99079862998804 23.518635735255287 56-57 23.237282489251996 26.539095461975514 26.928059283802863 23.295562764969624 58-59 23.488477713097893 26.185284331892717 27.00363284534715 23.32260510966224 60-61 23.286564438213116 26.242335654561654 27.326946924392686 23.144152982832544 62-63 23.06250286654182 26.425450863044457 27.344078931882233 23.167967338531493 64-65 22.960307070828684 26.353757226556006 27.178016074467543 23.50791962814777 66-67 23.000767605028614 26.406681125412874 26.977432606366218 23.615118663192295 68-69 22.979630535622587 26.242532471079826 27.29765591520553 23.480181078092052 70-71 23.135729633271424 26.245626813309542 27.076125617587433 23.542517935831604 72-73 23.10601784859018 26.218755075035883 27.138591295055697 23.536635781318235 74-75 22.925642810879257 26.674527317095432 26.840042803887698 23.559787068137613 76-77 23.1685953381809 26.339739046860895 26.818250216004735 23.673415398953466 78-79 23.154652602809925 26.41071269124606 26.91830818325459 23.516326522689422 80-81 23.232572258585204 26.529433146765115 26.827945534956427 23.410049059693257 82-83 23.252756672170786 26.440051177658898 26.81349665141627 23.49369549875405 84-85 23.252469016113732 26.510451563554486 26.713132081350793 23.52394733898098 86-87 23.228976267277304 26.425356320879533 26.89959337242679 23.446074039416374 88-89 23.126708618426672 26.4285651876762 26.692017645368477 23.75270854852865 90-91 23.17245226462939 26.400309553482693 26.739235661182644 23.688002520705272 92-93 23.225085677482692 26.318093176219715 26.806412950779357 23.650408195518235 94-95 23.181654877095543 26.15155869944119 26.90726454438203 23.759521879081237 96-97 23.16333037901888 26.02338350812825 27.00998373791905 23.803302374933818 98-99 23.076306854843214 26.237330053886566 26.67717630311643 24.00918678815379 100-101 23.44521772727979 25.492405562200027 27.11866175579752 23.943714954722665 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1748.0 1 968.5 2 245.0 3 305.0 4 299.0 5 305.5 6 458.5 7 942.5 8 1482.0 9 1810.5 10 1843.5 11 1772.0 12 1899.5 13 2084.5 14 2326.5 15 2529.5 16 2699.0 17 2838.5 18 2970.5 19 3117.5 20 3268.0 21 3621.5 22 4158.5 23 4898.0 24 5872.5 25 7280.0 26 9258.0 27 11637.5 28 14042.5 29 16550.5 30 19091.5 31 22245.0 32 26322.5 33 29192.0 34 31219.0 35 33633.0 36 36994.5 37 41340.0 38 44821.5 39 47569.5 40 50872.5 41 55844.0 42 60373.0 43 63509.0 44 67289.5 45 71593.5 46 74799.0 47 79469.0 48 87285.5 49 87955.0 50 83103.5 51 80966.5 52 79641.5 53 79953.0 54 79694.0 55 75072.5 56 68866.0 57 63084.5 58 57418.5 59 50839.0 60 43163.5 61 36118.5 62 29786.0 63 23369.5 64 17844.5 65 13739.5 66 10439.0 67 8337.5 68 6431.5 69 4678.5 70 3568.5 71 2705.5 72 2098.0 73 1658.5 74 1256.5 75 987.0 76 724.0 77 460.5 78 306.5 79 197.5 80 125.0 81 94.0 82 76.5 83 53.5 84 36.5 85 31.5 86 21.0 87 18.5 88 17.5 89 11.5 90 9.5 91 8.0 92 7.0 93 5.0 94 3.0 95 3.0 96 3.5 97 2.5 98 1.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3887356481696104 2 0.11420618731939486 3 0.11579976202617712 4 0.12796887796887796 5 0.1300453541019579 6 0.07171086180520142 7 0.08247956361163908 8 0.07914754377018528 9 0.07905096348492575 10-11 0.07697448735184585 12-13 0.08136889033115448 14-15 0.07448754500641293 16-17 0.07596039435662076 18-19 0.0856425679538887 20-21 0.08337293125028974 22-23 0.09317583020413209 24-25 0.10628660392811337 26-27 0.10795261384884028 28-29 0.10157831502171126 30-31 0.09257220342126002 32-33 0.09071303293001406 34-35 0.3380792885509866 36-37 0.5308776830003246 38-39 0.5110304343794909 40-41 0.48181489808848305 42-43 0.5112718850926399 44-45 0.521123074189112 46-47 0.5179600698468623 48-49 0.5254208968831611 50-51 0.5236824517484895 52-53 0.5222820376122264 54-55 0.5161250444269312 56-57 0.4869819433498679 58-59 0.4779275416067869 60-61 0.4265468298487166 62-63 0.5025313692766523 64-65 0.5295255590066911 66-67 0.5390628621760697 68-69 0.5545398528889095 70-71 0.5500730146956562 72-73 0.5360930184043391 74-75 0.5403666960270734 76-77 0.515545562715374 78-79 0.4886962434132246 80-81 0.4956983140945405 82-83 0.5090746836029855 84-85 0.5036903326997666 86-87 0.5048251510515661 88-89 0.515352402144855 90-91 0.5348133296246503 92-93 0.5409703228099454 94-95 0.5353445211935778 96-97 0.5368173705437856 98-99 0.53693809590036 100-101 0.5130344752986262 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2070816.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.0796127226776 #Duplication Level Percentage of deduplicated Percentage of total 1 79.51479195982132 48.56732698630166 2 11.968866731835837 14.621074894197456 3 3.653702226549754 6.695001510049316 4 1.6251111631791426 3.9704464191312865 5 0.8721911494956572 2.6636548815670875 6 0.5176853739691256 1.8972013292537226 7 0.3571019572697631 1.526815447678311 8 0.24452960412555041 1.194861881537463 9 0.1907782188347711 1.048739375011505 >10 0.9698832801245666 10.56352997488138 >50 0.055721096061342304 2.315311142924216 >100 0.027023722569211695 3.0869106869039094 >500 0.0019799364878836214 0.852679711965895 >1k 5.543822166074059E-4 0.7513372262015168 >5k 7.919745951534368E-5 0.24510853239525401 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5067 0.24468615270502062 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4245 0.20499165546335357 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3168 0.1529831718510964 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2562 0.12371934541745863 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 4.829014262976527E-5 6 0.0 0.0 0.0 0.0 4.829014262976527E-5 7 0.0 0.0 0.0 0.0 4.829014262976527E-5 8 0.0 0.0 0.0 0.0 4.829014262976527E-5 9 0.0 0.0 0.0 4.829014262976527E-5 4.829014262976527E-5 10-11 0.0 0.0 0.0 4.829014262976527E-5 4.829014262976527E-5 12-13 0.0 0.0 0.0 4.829014262976527E-5 9.658028525953054E-5 14-15 0.0 0.0 0.0 7.243521394464791E-5 9.658028525953054E-5 16-17 0.0 0.0 0.0 9.658028525953054E-5 9.658028525953054E-5 18-19 0.0 0.0 0.0 1.2072535657441318E-4 9.658028525953054E-5 20-21 0.0 0.0 0.0 1.6901549920417844E-4 2.1730564183394373E-4 22-23 0.0 0.0 0.0 2.8974085577859163E-4 2.4145071314882636E-4 24-25 0.0 0.0 0.0 3.621760697232395E-4 2.8974085577859163E-4 26-27 0.0 0.0 0.0 5.553366402423006E-4 2.8974085577859163E-4 28-29 0.0 0.0 0.0 0.0021730564183394373 2.8974085577859163E-4 30-31 0.0 0.0 0.0 0.006615749540277842 2.8974085577859163E-4 32-33 0.0 0.0 0.0 0.01564600621204395 2.8974085577859163E-4 34-35 0.0 0.0 0.0 0.02957771236073123 2.8974085577859163E-4 36-37 0.0 0.0 0.0 0.05251553010986973 2.8974085577859163E-4 38-39 0.0 0.0 0.0 0.08602888909492683 2.8974085577859163E-4 40-41 0.0 0.0 0.0 0.12272939749354844 2.8974085577859163E-4 42-43 0.0 0.0 0.0 0.16056472424396953 2.8974085577859163E-4 44-45 0.0 0.0 0.0 0.2088307218024199 2.8974085577859163E-4 46-47 0.0 0.0 0.0 0.2673583746696955 3.138859270934743E-4 48-49 0.0 0.0 0.0 0.3348197039234775 4.104662123530048E-4 50-51 0.0 0.0 0.0 0.4056371980900283 4.3461128366788747E-4 52-53 0.0 0.0 0.0 0.4786036036036036 4.3461128366788747E-4 54-55 0.0 0.0 0.0 0.5627491771359696 4.3461128366788747E-4 56-57 0.0 0.0 0.0 0.6619129850261926 4.3461128366788747E-4 58-59 0.0 0.0 0.0 0.7735356497148951 4.829014262976527E-4 60-61 0.0 0.0 0.0 0.8973515754176131 4.829014262976527E-4 62-63 0.0 0.0 0.0 1.0272279140203668 4.829014262976527E-4 64-65 0.0 0.0 0.0 1.1702150263471018 4.829014262976527E-4 66-67 0.0 0.0 0.0 1.3322284548699643 4.829014262976527E-4 68-69 0.0 0.0 0.0 1.519111306847156 4.829014262976527E-4 70-71 0.0 0.0 0.0 1.725479231375458 4.829014262976527E-4 72-73 0.0 0.0 0.0 1.937738553304591 5.553366402423006E-4 74-75 0.0 0.0 0.0 2.163205229242965 6.519169255018312E-4 76-77 0.0 0.0 0.0 2.409895422867121 7.24352139446479E-4 78-79 0.0 0.0 0.0 2.697946123653671 7.24352139446479E-4 80-81 0.0 0.0 0.0 3.0087656266901552 7.24352139446479E-4 82-83 0.0 0.0 0.0 3.3344826387279216 7.96787353391127E-4 84-85 0.0 0.0 0.0 3.674638403411988 8.209324247060096E-4 86-87 0.0 0.0 0.0 4.024234890980174 8.209324247060096E-4 88-89 0.0 0.0 0.0 4.415457481495217 8.209324247060096E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9890 0.0 26.985449 1 ATCAACG 11275 0.0 23.526484 3 TCAACGC 11325 0.0 23.422617 4 CAACGCA 11360 0.0 23.30819 5 TATCAAC 11585 0.0 23.059938 2 GTACATG 7435 0.0 22.92995 1 AACGCAG 11685 0.0 22.72944 6 GGTATCA 7155 0.0 22.566225 1 TACATGG 7495 0.0 21.92971 2 GAGTACT 5215 0.0 20.698822 12-13 ACATGGG 7750 0.0 20.597466 3 ACGCAGA 12925 0.0 20.475563 7 AGAGTAC 9090 0.0 20.207956 10-11 CGCAGAG 13070 0.0 20.139738 8 GTACTTT 5490 0.0 19.446873 14-15 GCAGAGT 13940 0.0 18.339418 9 AGTACTT 5495 0.0 18.30865 12-13 CATGGGG 4645 0.0 17.7439 4 GAGTACA 6030 0.0 17.089607 1 TACCTGG 1695 0.0 16.767048 2 >>END_MODULE