FastQCFastQC Report
Fri 27 May 2016
SRR939084_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939084_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2070816
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52750.2547305023720118No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46570.22488719422681688No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35850.1731201613277085No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26950.1301419343872174No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75150.027.896681
GTATCAA99750.023.5962451
GTACATG70200.023.2801531
TACATGG70550.022.5304182
GAGTACT60400.021.8802212-13
GTACTTT62650.021.05654514-15
ACATGGG72900.020.697653
TCAACGC116900.019.8479584
CAACGCA117350.019.812285
TATCAAC118500.019.6600512
ATCAACG118050.019.6546063
AACGCAG119700.019.3836776
AGAGTAC97100.018.98584610-11
GCACCGT4101.8189894E-1218.5164456
AGTACTT62300.018.31852312-13
CATGGGG43900.017.941764
ACTTTTT74550.017.63090316-17
TACCTGG15650.017.5847072
ACGCAGA131700.017.5814847
CGCAGAG132500.017.36798
Produced by FastQC (version 0.11.5)