##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939083_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 836443 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1957730532744 33.0 31.0 34.0 31.0 34.0 2 32.268983062802846 34.0 31.0 34.0 31.0 34.0 3 32.29817811853288 34.0 31.0 34.0 30.0 34.0 4 35.75712272085486 37.0 35.0 37.0 35.0 37.0 5 35.817613393859475 37.0 35.0 37.0 35.0 37.0 6 35.93535961207159 37.0 37.0 37.0 35.0 37.0 7 35.87722534589924 37.0 37.0 37.0 35.0 37.0 8 35.873830015912624 37.0 37.0 37.0 35.0 37.0 9 37.55516155912596 39.0 38.0 39.0 35.0 39.0 10-11 37.488904205068366 39.0 38.0 39.0 35.0 39.0 12-13 37.40632236745361 39.0 38.0 39.0 35.0 39.0 14-15 38.672637585585626 40.0 38.0 41.0 34.5 41.0 16-17 38.622991046610466 40.0 38.0 41.0 34.5 41.0 18-19 38.631414214716365 40.0 38.0 41.0 35.0 41.0 20-21 38.55804818738396 40.0 38.0 41.0 34.0 41.0 22-23 38.467510039536464 40.0 38.0 41.0 34.0 41.0 24-25 38.46488164764365 40.0 38.0 41.0 34.0 41.0 26-27 38.336866947299455 40.0 38.0 41.0 34.0 41.0 28-29 38.19535581025844 40.0 38.0 41.0 34.0 41.0 30-31 37.96405493261346 40.0 37.5 41.0 34.0 41.0 32-33 37.661356482151206 40.0 37.0 41.0 33.0 41.0 34-35 37.30561197834162 40.0 36.0 41.0 33.0 41.0 36-37 36.989978396615186 40.0 35.5 41.0 31.5 41.0 38-39 36.70228455495473 39.5 35.0 41.0 31.0 41.0 40-41 36.50270550414075 39.0 35.0 41.0 31.0 41.0 42-43 36.23238044911608 39.0 35.0 41.0 30.0 41.0 44-45 35.863358292196835 39.0 35.0 41.0 29.5 41.0 46-47 35.3313692624602 39.0 35.0 41.0 26.5 41.0 48-49 35.105032859381936 39.0 35.0 41.0 24.0 41.0 50-51 34.02197579512292 38.0 34.0 40.0 22.5 40.5 52-53 34.138215634538156 38.0 34.5 39.5 23.0 40.5 54-55 34.59196323001089 39.0 35.0 40.5 23.0 41.0 56-57 34.448787305291575 39.0 35.0 41.0 21.5 41.0 58-59 34.24353542321473 38.0 35.0 41.0 20.0 41.0 60-61 33.92615755048461 38.0 34.5 40.0 18.5 41.0 62-63 33.547962024907854 37.0 34.0 40.0 15.5 41.0 64-65 33.15939878748462 37.0 34.0 40.0 10.5 41.0 66-67 32.742124089746696 36.0 33.0 39.0 7.5 41.0 68-69 32.32584348246085 35.5 33.0 39.0 2.0 41.0 70-71 31.879108319395346 35.0 33.0 38.5 2.0 40.0 72-73 31.414498059042877 35.0 32.0 37.5 2.0 40.0 74-75 30.9694007840343 35.0 32.0 37.0 2.0 39.0 76-77 30.524368665886378 35.0 31.0 36.5 2.0 39.0 78-79 30.081587747162686 35.0 31.0 36.0 2.0 38.5 80-81 29.655725494743812 35.0 31.0 35.5 2.0 37.0 82-83 29.22309768866498 35.0 30.0 35.0 2.0 37.0 84-85 28.840267657210354 34.0 29.5 35.0 2.0 36.5 86-87 28.46752976592547 34.0 29.0 35.0 2.0 36.0 88-89 28.04053653387021 34.0 29.0 35.0 2.0 36.0 90-91 27.647891727230665 34.0 28.0 35.0 2.0 35.0 92-93 27.25513274664263 34.0 27.0 35.0 2.0 35.0 94-95 26.78916734314233 34.0 25.5 35.0 2.0 35.0 96-97 26.241708042269465 34.0 24.5 35.0 2.0 35.0 98-99 25.601133609821588 33.5 21.0 35.0 2.0 35.0 100-101 23.23145629767958 30.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4124704196607283 1101 2 0.4408024110589679 1101 3 0.4377947731503724 1101 4 0.3380503360938718 1101 5 0.2875817472808606 1101 6 0.27442810448610544 1101 7 0.3030011843943825 1101 8 0.4171554751160116 1101 9 0.4231166463609952 1101 10-11 0.4025262017852569 1101 12-13 0.3931336100458296 1101 14-15 0.38971467800874393 1101 16-17 0.5604054472907976 1101 18-19 0.46709839916092477 1101 20-21 0.38735796574510317 1101 22-23 0.5670990766435153 1101 24-25 0.49153889904592774 1101 26-27 0.41934077382151713 1101 28-29 0.353993898446582 1101 30-31 0.4478641312328122 1101 32-33 0.4277991936593324 1101 34-35 0.4000088145469505 1101 36-37 0.4615616808228822 1101 38-39 0.6386954096184709 1101 40-41 0.29846421840243664 1101 42-43 0.2365003568809385 1101 44-45 0.12844227665132024 1101 46-47 0.04577849419038671 1101 48-49 0.41437473233907696 1101 50-51 0.3087239777864994 1101 52-53 0.2655396682420843 1101 54-55 0.4121191958198196 1101 56-57 0.21869198870880524 1101 58-59 0.18775110924617167 1101 60-61 0.1726928698299801 1101 62-63 0.3656859066885474 1101 64-65 0.3216527072770532 1101 66-67 0.12504921779017764 1101 68-69 0.21377801551024334 1101 70-71 0.2522622259909326 1101 72-73 0.026491711928304795 1101 74-75 0.033262095020869964 1101 76-77 0.06007114751718845 1101 78-79 0.10256837365270499 1101 80-81 0.036677683355286206 1101 82-83 0.08084729619435649 1101 84-85 0.16561382785554812 1101 86-87 -0.008879945728725858 1101 88-89 0.1430340118874085 1101 90-91 0.10108039417963965 1101 92-93 0.4059076605464291 1101 94-95 0.39673481563983515 1101 96-97 0.473677898218245 1101 98-99 0.2255596640314721 1101 100-101 -0.12994552917728797 1102 1 0.2525890858336197 1102 2 0.21561040371680207 1102 3 0.37461348923712023 1102 4 0.29181963746086836 1102 5 0.26048335257495125 1102 6 0.36457494783709876 1102 7 0.3371438736242638 1102 8 0.39284662104159196 1102 9 0.41526326429456617 1102 10-11 0.3706276235251593 1102 12-13 0.4538514035312673 1102 14-15 0.5744724347833454 1102 16-17 0.6526152626095225 1102 18-19 0.6298920456098003 1102 20-21 0.5389592345231762 1102 22-23 0.6041661544286256 1102 24-25 0.5465950923896656 1102 26-27 0.48922621346599726 1102 28-29 0.5239807759322161 1102 30-31 0.5917207013456789 1102 32-33 0.5491502490959235 1102 34-35 0.43091716626253174 1102 36-37 0.6251744780921129 1102 38-39 0.5625526237811229 1102 40-41 0.34447956288856574 1102 42-43 0.3768126405204697 1102 44-45 0.310239417070278 1102 46-47 0.48328553848491396 1102 48-49 0.37233639527562445 1102 50-51 0.3473830661340287 1102 52-53 0.6340065882350032 1102 54-55 0.6675131127695977 1102 56-57 0.5362709250605917 1102 58-59 0.47683084260955866 1102 60-61 0.33022342194166043 1102 62-63 0.2961305425588989 1102 64-65 0.3454604101124801 1102 66-67 0.3124093382274893 1102 68-69 0.1658285090133944 1102 70-71 0.4756770584574852 1102 72-73 0.4557267417961093 1102 74-75 0.24475194318613092 1102 76-77 0.17207543044825613 1102 78-79 0.1492197173015697 1102 80-81 -0.1955626438220044 1102 82-83 -0.059704493458983876 1102 84-85 -0.17985246418882994 1102 86-87 0.007790880426135516 1102 88-89 -0.25547380526685615 1102 90-91 -0.28820543511496766 1102 92-93 -0.39245242768839006 1102 94-95 -0.27783689125311284 1102 96-97 -0.3811874953766825 1102 98-99 -0.3614333480283598 1102 100-101 -0.5208938119795476 1103 1 0.4123782635207718 1103 2 0.4827628662710879 1103 3 0.33298383049109503 1103 4 0.4078909647948237 1103 5 0.39751029686086525 1103 6 0.41980147410673396 1103 7 0.34439301661530664 1103 8 0.4190123232975935 1103 9 0.3944796160631938 1103 10-11 0.5026126716952959 1103 12-13 0.3681514530431471 1103 14-15 0.5392566423854959 1103 16-17 0.5897238447935607 1103 18-19 0.6345059096902546 1103 20-21 0.6916183244873864 1103 22-23 0.5126495036989809 1103 24-25 0.525714121201041 1103 26-27 0.4296475557291046 1103 28-29 0.5078544564029883 1103 30-31 0.6301783875915845 1103 32-33 0.6459837569176656 1103 34-35 0.6420059361381547 1103 36-37 0.5485217830965894 1103 38-39 0.5871781666164466 1103 40-41 0.3371217583433008 1103 42-43 0.3401946416316477 1103 44-45 0.2582755720764567 1103 46-47 0.16118151063732 1103 48-49 0.25759262866930754 1103 50-51 0.312120029582168 1103 52-53 0.4516980120982126 1103 54-55 0.2513988877437967 1103 56-57 0.23888692835633663 1103 58-59 0.20226766205752256 1103 60-61 0.18760843911963576 1103 62-63 0.19160001716610964 1103 64-65 0.07085565470491417 1103 66-67 0.11451302591716939 1103 68-69 -0.05891004183833104 1103 70-71 0.009100407652255171 1103 72-73 -0.04687830085591216 1103 74-75 0.11906926516071081 1103 76-77 0.23804622222248284 1103 78-79 0.2022607290067775 1103 80-81 -0.1138642731783861 1103 82-83 -0.1817937811305299 1103 84-85 -0.07379311797232901 1103 86-87 -0.19163047317556803 1103 88-89 -0.019620594743127384 1103 90-91 0.08258936273674777 1103 92-93 0.051716320870664134 1103 94-95 -0.10070343974640039 1103 96-97 -0.07230899600219587 1103 98-99 -0.2717393767603653 1103 100-101 -0.26685916122723086 1104 1 0.15480004118188617 1104 2 0.18902656881360969 1104 3 0.2036041004566016 1104 4 -0.05589920006085691 1104 5 0.05517872953031144 1104 6 0.06443377458107591 1104 7 0.22553351181086612 1104 8 0.07838698824385659 1104 9 0.2078144775496682 1104 10-11 0.25890151482174417 1104 12-13 0.04451727072214595 1104 14-15 0.25428997214492455 1104 16-17 0.44778121708494467 1104 18-19 0.42875241311566725 1104 20-21 0.31618252355644216 1104 22-23 0.38405541691221146 1104 24-25 0.35154071032625467 1104 26-27 0.41396796507697076 1104 28-29 0.29505050489474627 1104 30-31 0.3181292081728486 1104 32-33 0.524186870652926 1104 34-35 0.5562691506575774 1104 36-37 0.4767528102742986 1104 38-39 0.6404161259404688 1104 40-41 0.5266993225904315 1104 42-43 0.6219152188298764 1104 44-45 0.9557465402477376 1104 46-47 0.9160081330368399 1104 48-49 0.9110888335502736 1104 50-51 1.0152217491241728 1104 52-53 0.9712873332654723 1104 54-55 0.9362052867618402 1104 56-57 0.805828354492931 1104 58-59 0.8512644418444921 1104 60-61 0.5913939455730031 1104 62-63 0.7866924272977656 1104 64-65 0.7278539798983701 1104 66-67 0.7318419128178206 1104 68-69 0.6438783446907621 1104 70-71 0.7048559006849899 1104 72-73 0.7720383248340674 1104 74-75 0.832658859916684 1104 76-77 0.9572416126224788 1104 78-79 0.6027160323759873 1104 80-81 0.6602660659606947 1104 82-83 0.6828971930301257 1104 84-85 0.8067854112295656 1104 86-87 0.42130897868575445 1104 88-89 0.4839268155918681 1104 90-91 0.763451832651235 1104 92-93 0.32697521342793934 1104 94-95 0.09572955505171876 1104 96-97 0.29157137918404885 1104 98-99 -0.0836711718350287 1104 100-101 -0.20961626991219262 1105 1 0.25356329455964755 1105 2 0.2237324179474669 1105 3 0.25300337478563506 1105 4 0.30718687806877654 1105 5 0.19478921081675793 1105 6 0.20149240710073713 1105 7 0.1528993544503905 1105 8 0.2357933498651832 1105 9 0.23145172677670445 1105 10-11 0.21264704996878692 1105 12-13 0.38614028889215746 1105 14-15 0.4605838265307227 1105 16-17 0.3319544604388369 1105 18-19 0.4034209904335029 1105 20-21 0.5013962717740341 1105 22-23 0.3244293351118728 1105 24-25 0.23059966756250105 1105 26-27 0.2002903380492569 1105 28-29 0.2243628822436392 1105 30-31 0.30204514893325296 1105 32-33 0.5218309106918895 1105 34-35 0.495234787172663 1105 36-37 0.4089470472609307 1105 38-39 0.46015607031510086 1105 40-41 0.36508046926350346 1105 42-43 0.3294426432626807 1105 44-45 0.26517143895028283 1105 46-47 0.5990071024146602 1105 48-49 0.5631550192599235 1105 50-51 0.5231808348352232 1105 52-53 0.5664434089950277 1105 54-55 0.4963572682388744 1105 56-57 0.4391350987150986 1105 58-59 0.5948724665794316 1105 60-61 0.7712657531148963 1105 62-63 0.7125741034000441 1105 64-65 0.6685296727447181 1105 66-67 0.6053683348646643 1105 68-69 0.5539424409198119 1105 70-71 0.47630307776846337 1105 72-73 0.41535481055359824 1105 74-75 0.5326630953503191 1105 76-77 0.530304607638783 1105 78-79 0.2665108397343481 1105 80-81 0.20741879569767718 1105 82-83 0.26103388459172905 1105 84-85 0.4697896043088683 1105 86-87 0.5979142080344886 1105 88-89 0.6109351170418016 1105 90-91 0.5304059205505993 1105 92-93 0.6694188468652769 1105 94-95 0.671376164587091 1105 96-97 0.41750670411240165 1105 98-99 0.13997384235367605 1105 100-101 0.03146990704422237 1106 1 0.2257663390200264 1106 2 0.1997477407929864 1106 3 0.20565739083239976 1106 4 0.14924125205077132 1106 5 0.294349164968871 1106 6 0.1805759856487512 1106 7 0.16575720590056164 1106 8 0.20714680085687576 1106 9 0.3726769511565209 1106 10-11 0.33134212802414 1106 12-13 0.2239986430603622 1106 14-15 0.2520463338201324 1106 16-17 0.3914452741047256 1106 18-19 0.4945017671663692 1106 20-21 0.28196571724253516 1106 22-23 0.32268406568852015 1106 24-25 0.5769682000950596 1106 26-27 0.4194351811677137 1106 28-29 0.29747567876349024 1106 30-31 0.30356670883378456 1106 32-33 0.12759691890965286 1106 34-35 0.27353194715094276 1106 36-37 0.23434469722171514 1106 38-39 0.2308781074626154 1106 40-41 0.30762733934105313 1106 42-43 0.38754930662258147 1106 44-45 0.36652358287510367 1106 46-47 0.33063443418844685 1106 48-49 0.5842490677487504 1106 50-51 0.5030385991476081 1106 52-53 0.5531951132091493 1106 54-55 0.5033073449871566 1106 56-57 0.5938415449008048 1106 58-59 0.3842034494266926 1106 60-61 0.43722973169240475 1106 62-63 0.4230696828328391 1106 64-65 0.4714491239106877 1106 66-67 0.29377197052144766 1106 68-69 0.3587353546064804 1106 70-71 0.47324739001669514 1106 72-73 0.3942373018900902 1106 74-75 0.1704312954186875 1106 76-77 0.24817170910068498 1106 78-79 0.18452423214263902 1106 80-81 0.3582702324657987 1106 82-83 0.44472872804533736 1106 84-85 0.5101193203066963 1106 86-87 0.3675277519284741 1106 88-89 0.35975121608395355 1106 90-91 0.41565969261154123 1106 92-93 0.3253510871779035 1106 94-95 0.33890610982433955 1106 96-97 0.8267099630658095 1106 98-99 0.6758612067035941 1106 100-101 0.3874805713693412 1107 1 0.17777737234961677 1107 2 0.14134931535425466 1107 3 0.1404818915378172 1107 4 0.1723783088689359 1107 5 0.1485138193618596 1107 6 0.17508148885440278 1107 7 0.09324251564292041 1107 8 0.1287306726878441 1107 9 0.2908390340616691 1107 10-11 0.23505621784570252 1107 12-13 0.3248218675224379 1107 14-15 0.3822769918455009 1107 16-17 0.2999935710482333 1107 18-19 0.29784549306857144 1107 20-21 0.23225081353873378 1107 22-23 0.2739896816342764 1107 24-25 0.28130885256259575 1107 26-27 0.33793215919448016 1107 28-29 0.1873110180367945 1107 30-31 0.23463690063447018 1107 32-33 0.24284755188518403 1107 34-35 0.08711570497506216 1107 36-37 0.20938432251920602 1107 38-39 0.20136300076328695 1107 40-41 0.19860908082735307 1107 42-43 0.004155354369288489 1107 44-45 0.1177226340847568 1107 46-47 -0.03482798234507811 1107 48-49 -0.19968895052718239 1107 50-51 -0.2022141081192146 1107 52-53 -0.14388947829005616 1107 54-55 -0.05473401733136285 1107 56-57 -0.35956290548325853 1107 58-59 -0.17394241917091335 1107 60-61 -0.2800720745244476 1107 62-63 -0.3332169240541276 1107 64-65 -0.19125844376900147 1107 66-67 -0.0848146928939073 1107 68-69 -0.13183295370605208 1107 70-71 -0.42491124101439226 1107 72-73 -0.4831427890303708 1107 74-75 -0.4333505651414349 1107 76-77 -0.3466756563118487 1107 78-79 -0.39004258141341097 1107 80-81 -0.2835637893950107 1107 82-83 -0.11494514902677722 1107 84-85 -0.25925669803573825 1107 86-87 -0.11566336313768844 1107 88-89 -0.1607074714755221 1107 90-91 -0.11946866801746125 1107 92-93 -0.1040169190164697 1107 94-95 -0.2410728622455629 1107 96-97 0.04925133409318505 1107 98-99 0.07858090344537061 1107 100-101 0.04533888935661423 1108 1 0.009199589264561325 1108 2 0.12382087997197999 1108 3 0.10455777893071883 1108 4 0.07009601493216167 1108 5 0.07048742893883286 1108 6 0.09285472227829672 1108 7 0.23226242787387008 1108 8 0.3029865762015902 1108 9 0.1973306601774354 1108 10-11 0.19016189895147306 1108 12-13 0.1921114755470299 1108 14-15 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-1.2594287388391159 2316 98-99 -1.104498411581126 2316 100-101 -0.7958270592818977 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3258.0 3 591.0 4 555.0 5 588.0 6 685.0 7 823.0 8 1084.0 9 1290.0 10 1687.0 11 2386.0 12 3525.0 13 5827.0 14 5377.0 15 6232.0 16 8235.0 17 11955.0 18 11040.0 19 6037.0 20 5881.0 21 5988.0 22 5867.0 23 6382.0 24 7048.0 25 7607.0 26 8655.0 27 10124.0 28 11598.0 29 13904.0 30 16669.0 31 19907.0 32 24720.0 33 31987.0 34 45025.0 35 71824.0 36 109302.0 37 167110.0 38 166574.0 39 29089.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.02886155211813 20.06153772334415 15.156153472442977 20.75344725209474 2 15.649742602130278 22.29660421531652 38.957864788617755 23.09578839393544 3 19.415391133563844 26.92341295915302 31.228449385003344 22.43274652227979 4 12.584817020257939 17.942846883509382 37.95206319758221 31.520272898650468 5 12.645989857924752 38.48709255721079 33.76417999071028 15.102737594154181 6 28.627290969419438 37.27604827892362 17.69848287778186 16.39817787387508 7 25.756254001304352 35.709644414927034 21.3864407388128 17.147660844955816 8 25.224956767850838 32.889463322981555 20.731206730452787 21.15437317871482 9 26.45349811178067 17.694098895786567 22.172577024510954 33.679825967921815 10-11 24.336469554958065 26.943984760427846 26.89588246767155 21.82366321694254 12-13 26.671341440795697 25.517685364566567 27.32355619481363 20.4874169998241 14-15 23.36086980714496 26.658844688663535 27.728991796496143 22.251293707695353 16-17 20.082917301059798 29.80105231985977 28.626031605490727 21.489998773589704 18-19 19.909674454672086 28.12908367678102 32.056832813973415 19.90440905457348 20-21 21.433333014184566 26.671222515307175 31.405555103428135 20.489889367080124 22-23 20.801268720357875 26.674785679019735 31.5284788820898 20.995466718532594 24-25 21.104703534009868 27.175335849146947 31.73546249771811 19.98449811912507 26-27 20.873958411890115 26.562546759989132 32.478396136861775 20.085098691258978 28-29 19.886834619221954 27.58223612350516 32.46113157001704 20.069797687255846 30-31 20.138264264037826 27.30449915410086 31.877860183374896 20.67937639848642 32-33 20.069498175777607 27.70933079133014 31.481823899258167 20.73934713363409 34-35 21.195450085523806 27.109907087320646 31.104576543206914 20.590066283948634 36-37 21.310832378740706 26.589914816498965 31.63376801086524 20.46548479389509 38-39 20.838875773864824 27.08240125247646 31.51128916316004 20.567433810498677 40-41 20.320210575739 27.400723734020843 31.95195040134396 20.3271152888962 42-43 20.414554076892863 27.8197593059054 30.518048453530234 21.247638163671503 44-45 20.456696810401994 27.609267036335854 30.551164265738972 21.382871887523184 46-47 21.18800652581855 27.93307431888571 29.78990516419065 21.089013991105087 48-49 21.057254219693185 27.73282805951786 29.697232208081818 21.51268551270714 50-51 20.607927091517634 28.317691551756035 29.93112570369997 21.143255653026362 52-53 20.920249747405272 28.551734163421884 29.364242197796386 21.163773891376458 54-55 20.304983780206026 27.92597455405327 29.665075218881633 22.103966446859076 56-57 20.94889691289338 28.490507007526055 28.759050274398135 21.80154580518243 58-59 21.290649887877905 27.51449181823913 29.179482407187823 22.015375886695146 60-61 20.77982248772844 27.827800253383195 30.036831185697714 21.355546073190652 62-63 20.7711132797838 27.83490728924255 29.991201861722093 21.40277756925156 64-65 20.43997500961169 28.1370746828143 29.93476066897347 21.488189638600538 66-67 20.48761350432629 27.890552811278518 29.335754118117798 22.286079566277394 68-69 20.36898391223413 27.99910590141121 29.646698865432153 21.985211320922506 70-71 20.839619393690327 28.19237237269714 29.068605627143047 21.89940260646949 72-73 20.863314106788728 27.686885077489926 29.348546354275868 22.101254461445482 74-75 20.334687761540525 28.952840544166513 28.84271189251251 21.869759801780443 76-77 20.817425631242447 27.928258566132392 29.09757978678674 22.156736015838423 78-79 20.671284507691865 28.06893851313046 29.33472100777354 21.925055971404138 80-81 21.030410984900804 28.626430976228768 28.722640142884426 21.620517895985998 82-83 20.843834464532403 28.248723647065887 28.86984203255451 22.037599855847198 84-85 20.687650307883402 27.975825807428123 28.75352270024434 22.58300118444414 86-87 21.08700117846756 28.06236387858781 28.62474877978754 22.225886163157092 88-89 21.188714778737936 27.671319282373823 28.497856913294346 22.642109025593893 90-91 20.86794039894256 27.56981494832973 28.96959865416967 22.59264599855804 92-93 21.332022034151855 27.837611977600574 28.82613290301573 22.004233085231846 94-95 21.017127153811536 27.470676200243567 28.859497498547555 22.652699147397342 96-97 20.902820751599627 27.200036047943765 28.816125446844303 23.081017753612304 98-99 21.256826891479236 27.483528650565887 28.151754490834495 23.107889967120382 100-101 21.654209497882892 26.61122761028394 28.92433854265842 22.810224349174753 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3709.0 1 2277.5 2 1036.0 3 1192.0 4 970.0 5 992.0 6 1661.0 7 3117.5 8 4677.0 9 4954.0 10 3843.5 11 2893.0 12 2779.0 13 2801.0 14 2757.5 15 2733.5 16 2727.5 17 2689.0 18 2724.5 19 2817.5 20 2950.5 21 3221.0 22 3609.5 23 4008.5 24 4421.5 25 5205.5 26 6585.0 27 7916.5 28 8544.0 29 9116.5 30 10417.5 31 12175.0 32 14793.5 33 16957.0 34 18282.0 35 19424.5 36 20903.5 37 22891.5 38 23338.0 39 22445.5 40 21913.5 41 22971.5 42 23731.0 43 23996.5 44 24089.5 45 24514.5 46 25393.0 47 28897.5 48 43047.5 49 42832.0 50 28284.0 51 22495.5 52 20299.5 53 20703.0 54 21168.0 55 20377.5 56 18527.5 57 16532.5 58 15445.0 59 13993.0 60 11970.5 61 10639.5 62 9633.5 63 7744.0 64 5681.5 65 4531.5 66 3642.5 67 3262.5 68 2621.5 69 1738.0 70 1398.0 71 1197.5 72 1048.0 73 921.5 74 723.0 75 564.5 76 410.5 77 261.5 78 183.5 79 121.5 80 67.0 81 52.5 82 34.5 83 22.5 84 14.0 85 6.5 86 6.5 87 5.5 88 4.5 89 4.5 90 3.5 91 2.0 92 2.0 93 1.5 94 1.0 95 0.5 96 0.5 97 1.0 98 1.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33570727473360407 2 0.1154890410942527 3 0.10879402421922353 4 0.11572814883978944 5 0.13282435264566742 6 0.0856005729021583 7 0.09301291301379772 8 0.10018614537990038 9 0.08787209648475748 10-11 0.08655700388430533 12-13 0.0899045123218199 14-15 0.09127938185865624 16-17 0.08016087169119712 18-19 0.09552354434193364 20-21 0.10604428514555088 22-23 0.11423372543018472 24-25 0.12678688207086436 26-27 0.12672710513448018 28-29 0.12296115814227629 30-31 0.11255997121142744 32-33 0.10538673884532479 34-35 0.32961002722241683 36-37 0.4858071619943021 38-39 0.4605812948401744 40-41 0.4397789209784767 42-43 0.4703249354707972 44-45 0.47666129072752117 46-47 0.48503006182130765 48-49 0.4967463413526086 50-51 0.48503006182130765 52-53 0.48801890864051706 54-55 0.4749277595723797 56-57 0.4546036012017555 58-59 0.4358336431771203 60-61 0.3966199729090924 62-63 0.463928803277689 64-65 0.4929206174240205 66-67 0.5028435888637959 68-69 0.5164727303593909 70-71 0.5074464129653784 72-73 0.49895808799882363 74-75 0.5062508742376945 76-77 0.48263898436594005 78-79 0.4632712569774629 80-81 0.4641081340868415 82-83 0.47737861396413145 84-85 0.47642218298198447 86-87 0.47893281431012036 88-89 0.49202396337825766 90-91 0.5072670821562258 92-93 0.5008709499631175 94-95 0.5052944432555476 96-97 0.5042782353370164 98-99 0.4978821031439082 100-101 0.4850898387576918 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 836443.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.04533015423506 #Duplication Level Percentage of deduplicated Percentage of total 1 67.99376416877168 23.828639137238017 2 13.993485075900164 9.808126089865645 3 6.135267444678596 6.450374195498744 4 3.2852093701509775 4.605249880109105 5 1.9578330304434566 3.4306452469378748 6 1.2947143631119242 2.7224215386412536 7 0.9043667575885597 2.21856821201443 8 0.6616653838397296 1.8550625462633592 9 0.4783067207735334 1.5086175250048202 >10 2.8623226347878257 18.99468477666901 >50 0.27561792420949394 6.659885867102562 >100 0.1420781004544946 8.950663612165686 >500 0.006830677906464523 1.626131180846296 >1k 0.007855279592434202 5.438311227214213 >5k 6.830677906464524E-4 1.9026189644290241 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9040 1.0807670098261328 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6856 0.8196613516999962 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4879 0.583303345236914 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4604 0.5504260302256101 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3873 0.46303214923192615 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3090 0.36942146685428656 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3039 0.36332421934309933 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2494 0.2981673586843335 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2178 0.26038833488952623 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2007 0.2399446226461337 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1890 0.2259568195322335 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1563 0.18686270313697406 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1533 0.18327608695392275 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1527 0.18255876371731247 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1278 0.15278984939798648 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1264 0.1511160951792292 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1239 0.14812724836001975 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1207 0.14430152443143168 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1149 0.13736739981086576 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1145 0.13688918431979227 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1145 0.13688918431979227 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1116 0.13342212200950931 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1111 0.13282435264566742 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1078 0.12887907484431096 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1027 0.1227818273331237 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 967 0.11560859496702106 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 861 0.10293588445357303 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 840 0.10042525312543713 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.7933080915256627E-4 0.0 14-15 0.0 0.0 0.0 2.988846819209438E-4 0.0 16-17 0.0 0.0 0.0 5.379924274576988E-4 0.0 18-19 0.0 0.0 0.0 5.977693638418877E-4 0.0 20-21 0.0 0.0 0.0 0.0011357617912995865 0.0 22-23 0.0 0.0 0.0 0.0023910774553675502 0.0 24-25 0.0 0.0 0.0 0.00579836282926631 0.0 26-27 0.0 0.0 0.0 0.009564309821470201 0.0 28-29 0.0 0.0 0.0 0.02205768952576565 0.0 30-31 0.0 0.0 0.0 0.050391957371871125 0.0 32-33 0.0 0.0 0.0 0.09791462179730119 0.0 34-35 0.0 0.0 0.0 0.17054359950409054 0.0 36-37 0.0 0.0 0.0 0.2782018619320145 0.0 38-39 0.0 0.0 0.0 0.42065030133553627 0.0 40-41 0.0 0.0 0.0 0.5701524192323923 0.0 42-43 0.0 0.0 0.0 0.7152908207732027 0.0 44-45 0.0 0.0 0.0 0.8870897359413612 0.0 46-47 0.0 0.0 0.0 1.1037213533976613 0.0 48-49 0.0 0.0 0.0 1.3260915567468434 0.0 50-51 0.0 0.0 0.0 1.5591618317087956 0.0 52-53 0.0 0.0 0.0 1.7872108440144756 0.0 54-55 0.0 0.0 0.0 2.040067284919594 0.0 56-57 0.0 0.0 0.0 2.352461554463365 0.0 58-59 0.0 0.0 0.0 2.6749581262560627 0.0 60-61 0.0 0.0 0.0 3.039477884326846 0.0 62-63 0.0 0.0 0.0 3.3922813628663278 0.0 64-65 0.0 0.0 0.0 3.761344168102309 0.0 66-67 0.0 0.0 0.0 4.200405765844176 0.0 68-69 0.0 0.0 0.0 4.696255453151021 0.0 70-71 0.0 0.0 0.0 5.244170852048496 0.0 72-73 0.0 0.0 0.0 5.778277778641222 0.0 74-75 0.0 0.0 0.0 6.350044175155988 0.0 76-77 0.0 0.0 0.0 6.945661569288045 0.0 78-79 0.0 0.0 0.0 7.629569498459548 0.0 80-81 0.0 0.0 0.0 8.358609014601114 0.0 82-83 0.0 0.0 0.0 9.10689670425839 0.0 84-85 0.0 0.0 0.0 9.900017096203806 0.0 86-87 0.0 0.0 0.0 10.72475948749646 0.0 88-89 0.0 0.0 0.0 11.565820982421993 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATGC 40 5.4638466E-4 47.36431 8 GTATTGA 65 2.592511E-6 43.836323 1 GTCTATA 90 5.7054785E-7 36.93616 1 GTACATG 5310 0.0 25.309767 1 GAGTGCA 350 0.0 24.35733 9 TACATGG 5480 0.0 24.210884 2 GAGTACT 5780 0.0 24.132853 12-13 ACATGGG 5420 0.0 23.86693 3 GTACTTT 6065 0.0 22.650116 14-15 GTAAGGT 295 5.456968E-12 22.487398 4 CGCGGAA 180 0.0 22.451796 82-83 AGTACTT 5850 0.0 22.34624 12-13 CATGGGT 545 0.0 21.735855 4 GGACCTA 110 0.00291776 21.538256 5 AAATCGA 55 0.0029194753 21.537611 26-27 CATGGGG 1895 0.0 21.504158 4 CCTTTAG 225 7.1626346E-8 21.106379 1 AGGTAAG 270 1.031367E-9 21.059626 2 CATGGGA 3295 0.0 20.995712 4 TCGCGCT 195 0.0 20.727222 60-61 >>END_MODULE