##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939083_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 836443 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.43597830336317 34.0 31.0 34.0 31.0 34.0 2 32.63074112641268 34.0 31.0 34.0 31.0 34.0 3 32.69726807445337 34.0 31.0 34.0 31.0 34.0 4 36.18755731113776 37.0 35.0 37.0 35.0 37.0 5 36.16251914356388 37.0 35.0 37.0 35.0 37.0 6 36.219922935573614 37.0 37.0 37.0 35.0 37.0 7 36.17723502976294 37.0 37.0 37.0 35.0 37.0 8 36.1718180437878 37.0 37.0 37.0 35.0 37.0 9 37.883253252164224 39.0 38.0 39.0 35.0 39.0 10-11 37.83209316115982 39.0 38.0 39.0 35.0 39.0 12-13 37.77711033507364 39.0 38.0 39.0 35.0 39.0 14-15 39.04763683837392 40.0 38.0 41.0 36.0 41.0 16-17 39.0099540554467 40.0 38.0 41.0 36.0 41.0 18-19 39.092201142217704 40.5 38.5 41.0 35.5 41.0 20-21 39.16408828814396 41.0 39.0 41.0 35.5 41.0 22-23 39.10514045786742 41.0 39.0 41.0 35.0 41.0 24-25 39.02353358208509 41.0 38.5 41.0 35.0 41.0 26-27 38.83589377877512 40.0 38.0 41.0 35.0 41.0 28-29 38.65466983404727 40.0 38.0 41.0 35.0 41.0 30-31 38.37176771160736 40.0 37.5 41.0 34.0 41.0 32-33 38.09728158404099 40.0 37.0 41.0 34.0 41.0 34-35 37.94392385374736 40.0 37.0 41.0 33.5 41.0 36-37 37.75833918151028 40.0 36.5 41.0 33.0 41.0 38-39 37.548581911738154 40.0 36.0 41.0 33.0 41.0 40-41 37.33077448194318 40.0 36.0 41.0 33.0 41.0 42-43 37.078170299709605 40.0 35.5 41.0 32.5 41.0 44-45 36.725838461198194 40.0 35.0 41.0 31.0 41.0 46-47 36.23510508187647 40.0 35.0 41.0 30.0 41.0 48-49 35.75455589920652 39.5 35.0 41.0 28.5 41.0 50-51 35.42171313526445 39.0 35.0 41.0 27.0 41.0 52-53 35.14876805711806 39.0 35.0 41.0 26.0 41.0 54-55 34.87587319159823 39.0 35.0 40.5 25.5 41.0 56-57 34.57901733889817 38.0 35.0 40.0 24.5 41.0 58-59 34.27704278713553 38.0 34.0 40.0 23.0 41.0 60-61 33.88343437628147 37.5 34.0 40.0 22.0 41.0 62-63 33.480745848790654 37.0 34.0 40.0 20.5 41.0 64-65 33.05648980265242 36.0 33.0 39.0 19.5 41.0 66-67 32.66450015123564 36.0 33.0 39.0 17.0 40.5 68-69 32.18300709074019 35.0 32.5 38.5 10.5 40.0 70-71 31.740959037256573 35.0 32.0 37.5 7.5 40.0 72-73 31.28943036166242 35.0 31.5 37.0 3.5 39.0 74-75 30.823172648943203 35.0 31.0 36.5 2.0 39.0 76-77 28.881128182075766 33.0 28.5 35.0 2.0 37.0 78-79 29.797282660025846 34.0 30.0 35.0 2.0 37.0 80-81 29.82285583118037 34.0 31.0 35.0 2.0 37.0 82-83 29.536807648578566 34.0 30.5 35.0 2.0 37.0 84-85 29.182421276763627 34.0 30.0 35.0 2.0 36.0 86-87 28.67382475554222 34.0 29.0 35.0 2.0 36.0 88-89 28.12690045824999 34.0 29.0 35.0 2.0 35.0 90-91 27.681426588542195 34.0 27.5 35.0 2.0 35.0 92-93 27.43743745838031 34.0 27.0 35.0 2.0 35.0 94-95 27.137623842867953 34.0 27.0 35.0 2.0 35.0 96-97 26.60537657676614 33.0 25.5 35.0 2.0 35.0 98-99 25.90896749688861 33.0 24.0 35.0 2.0 35.0 100-101 22.533496604072244 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23030928527388284 1101 2 0.2512182641940299 1101 3 0.19323328636867387 1101 4 0.1374592219094808 1101 5 0.20136796236561594 1101 6 0.16895618547885505 1101 7 0.16760396441922154 1101 8 0.19415709486926147 1101 9 0.14734632168631379 1101 10-11 0.28564182795066984 1101 12-13 0.32479088238829235 1101 14-15 0.33303015015648185 1101 16-17 0.2709023556757799 1101 18-19 0.2410533327822577 1101 20-21 0.440134738793148 1101 22-23 0.356619695096569 1101 24-25 0.2358436910727164 1101 26-27 0.3161754335267517 1101 28-29 0.34620965989987695 1101 30-31 0.3628912215784368 1101 32-33 0.4591447348101454 1101 34-35 0.5768729524947531 1101 36-37 0.6585966433197896 1101 38-39 0.6696998772949243 1101 40-41 0.712993490995494 1101 42-43 0.7108715777933554 1101 44-45 0.43637259540787454 1101 46-47 0.3343636895662101 1101 48-49 0.5459074246539473 1101 50-51 0.4881829044661927 1101 52-53 0.6475890693143569 1101 54-55 0.7336802208137172 1101 56-57 0.6668507146962881 1101 58-59 0.537250667800464 1101 60-61 0.41770503222739563 1101 62-63 0.44288279588455026 1101 64-65 0.49120291731583166 1101 66-67 0.41856537826176066 1101 68-69 0.47037385460745895 1101 70-71 0.6666142097824128 1101 72-73 0.6361011272739354 1101 74-75 0.7898331357317296 1101 76-77 0.8485609959093949 1101 78-79 0.7799281419857671 1101 80-81 0.8861739787653633 1101 82-83 0.8333219032002397 1101 84-85 0.9271998839328965 1101 86-87 1.099505965349504 1101 88-89 1.4053695718435542 1101 90-91 1.0899157892840101 1101 92-93 0.8924086652498744 1101 94-95 0.946553168620472 1101 96-97 0.5961340938348556 1101 98-99 0.6727117720385998 1101 100-101 0.4429191830467367 1102 1 0.006079327298131432 1102 2 -0.012737489585738615 1102 3 0.01360780131867756 1102 4 0.10287777376353802 1102 5 0.053796030055252686 1102 6 0.14161224679239837 1102 7 -0.002755254348393521 1102 8 -0.056426575248437416 1102 9 0.0938257845487982 1102 10-11 0.17303576100169238 1102 12-13 -0.07270255635452116 1102 14-15 0.14517894175806845 1102 16-17 0.33557300471694873 1102 18-19 0.1932306235868353 1102 20-21 -0.01812680982456527 1102 22-23 0.12718098448424087 1102 24-25 0.035989004805465186 1102 26-27 0.0782272151294734 1102 28-29 0.07401006887149464 1102 30-31 0.07795755870629506 1102 32-33 0.08177181388335697 1102 34-35 0.1856348472892222 1102 36-37 0.20569278385144685 1102 38-39 0.12115261209131489 1102 40-41 0.1912507727833841 1102 42-43 0.05364896198514657 1102 44-45 0.17635560859479682 1102 46-47 0.1684604902488971 1102 48-49 0.0953845367347057 1102 50-51 0.046348650678375236 1102 52-53 0.12194582459154191 1102 54-55 0.2554551415150854 1102 56-57 0.1884867621627606 1102 58-59 0.16635044721341075 1102 60-61 0.20480431250175712 1102 62-63 0.21566404437506037 1102 64-65 0.13222969368564463 1102 66-67 0.35725586403631127 1102 68-69 0.4176161139341019 1102 70-71 0.16014754740941228 1102 72-73 0.3731738404946654 1102 74-75 0.326169619541929 1102 76-77 0.3331843072913614 1102 78-79 0.15215872791005935 1102 80-81 0.137677432482338 1102 82-83 0.2874434192934352 1102 84-85 0.37845739113820187 1102 86-87 0.4767621522527534 1102 88-89 0.5891371020736393 1102 90-91 0.6969085598251752 1102 92-93 0.7729072402897224 1102 94-95 0.6927975533114115 1102 96-97 0.6724700946077178 1102 98-99 0.2699471883362783 1102 100-101 0.01752228736670247 1103 1 0.18261750246433905 1103 2 0.05758448877266176 1103 3 0.18307258174674956 1103 4 0.09094390046102063 1103 5 0.15356245495782872 1103 6 0.05986096269629115 1103 7 0.15386289572172274 1103 8 0.14643982206356299 1103 9 0.1317817378791304 1103 10-11 0.18975924663757837 1103 12-13 0.17026562376286591 1103 14-15 0.15348351914966685 1103 16-17 0.26910236979330904 1103 18-19 0.2577973191039291 1103 20-21 0.1957484202544677 1103 22-23 0.1258940537290485 1103 24-25 0.22909570478241648 1103 26-27 0.24102112101155626 1103 28-29 0.12283690203916109 1103 30-31 0.03893199772921463 1103 32-33 0.07803007688589503 1103 34-35 0.30015845613786496 1103 36-37 0.3862438774591297 1103 38-39 0.28792542062399207 1103 40-41 0.5243645782810873 1103 42-43 0.34061467784746924 1103 44-45 0.35991143039740336 1103 46-47 0.37927902356359056 1103 48-49 0.3352355475706261 1103 50-51 0.1550149293130545 1103 52-53 0.2625283639081317 1103 54-55 0.5316161193556823 1103 56-57 0.5585927402490327 1103 58-59 0.5074371761415719 1103 60-61 0.3558731642420483 1103 62-63 0.48442658769794633 1103 64-65 0.5044361468591276 1103 66-67 0.39012834639534333 1103 68-69 0.3554796576991919 1103 70-71 0.2821064412158911 1103 72-73 0.27858726463756156 1103 74-75 0.12244621327621985 1103 76-77 0.04406142335702157 1103 78-79 -0.0219312551954296 1103 80-81 0.02614248881626935 1103 82-83 0.20808268939015306 1103 84-85 0.06336156109621172 1103 86-87 0.29516129354301057 1103 88-89 0.4556789811815172 1103 90-91 0.6027764727296336 1103 92-93 0.397570389471646 1103 94-95 0.2224980631703204 1103 96-97 0.017875884095325034 1103 98-99 -0.4839693520310142 1103 100-101 -0.49242544170130387 1104 1 0.10487024666998934 1104 2 0.10136237910795387 1104 3 0.14592736899455616 1104 4 0.07170580803979476 1104 5 0.07046358221725058 1104 6 0.22321190676218805 1104 7 0.16480829259171514 1104 8 0.12873049897378053 1104 9 0.20278725032186884 1104 10-11 0.23765668044944732 1104 12-13 0.184473750826335 1104 14-15 0.2886557506352858 1104 16-17 0.3201691808950997 1104 18-19 0.23753511760214963 1104 20-21 0.18304318250957863 1104 22-23 0.045890615384486466 1104 24-25 0.1941428988344498 1104 26-27 0.15087641476819158 1104 28-29 0.22697622523984506 1104 30-31 0.4086122686559719 1104 32-33 0.3962618065211032 1104 34-35 0.48998465336574526 1104 36-37 0.37402481424164336 1104 38-39 0.43866910127942305 1104 40-41 0.5124237252019199 1104 42-43 0.35344099708112253 1104 44-45 0.2848806516678195 1104 46-47 0.2042035093831842 1104 48-49 0.009648348564425646 1104 50-51 0.12295791194756589 1104 52-53 0.0825975744568197 1104 54-55 0.15689571592325535 1104 56-57 0.21539696534598818 1104 58-59 0.1382846726200455 1104 60-61 0.12215723805184098 1104 62-63 0.06219695064664421 1104 64-65 -0.005296586550862514 1104 66-67 -0.04736680110224256 1104 68-69 -0.15223998042428377 1104 70-71 -0.18954847469254688 1104 72-73 -0.004846014840698132 1104 74-75 -0.2336544451852518 1104 76-77 -0.011096278349953081 1104 78-79 0.11298125187535746 1104 80-81 -0.1383314629507808 1104 82-83 0.08947828756967979 1104 84-85 -0.09469692033857413 1104 86-87 0.19385398317880131 1104 88-89 0.17699468406418006 1104 90-91 0.23430239525966456 1104 92-93 0.17313152360775064 1104 94-95 0.2413941697072417 1104 96-97 0.20617331199176192 1104 98-99 0.39563313097136543 1104 100-101 0.11032283080783856 1105 1 -0.047379474497297736 1105 2 0.07586859813634561 1105 3 0.12100890109208962 1105 4 0.05304519333719071 1105 5 0.11375677305176879 1105 6 0.10067022303613982 1105 7 0.12139862733215523 1105 8 0.11964902334869976 1105 9 0.12352110979018249 1105 10-11 0.1472354665741662 1105 12-13 0.12341468064520456 1105 14-15 0.0817759945962635 1105 16-17 0.07548994440260515 1105 18-19 0.1384259704773001 1105 20-21 0.25636912736423767 1105 22-23 0.15689074244749435 1105 24-25 0.21762661359210966 1105 26-27 0.10450018119814786 1105 28-29 0.1254970000418396 1105 30-31 0.2245597351607458 1105 32-33 0.19034737639869803 1105 34-35 0.4915941181481145 1105 36-37 0.20075498888269294 1105 38-39 0.1190544551499002 1105 40-41 0.17797447275595601 1105 42-43 0.30434173263476794 1105 44-45 0.44218289221221596 1105 46-47 0.5401986951069517 1105 48-49 0.6000607386196251 1105 50-51 0.37135550622126345 1105 52-53 0.4287295646870888 1105 54-55 0.490842010624732 1105 56-57 0.5204664264576522 1105 58-59 0.7384509133903308 1105 60-61 1.0370744353242216 1105 62-63 0.9171033475632484 1105 64-65 0.6816960437946094 1105 66-67 0.6687338469190962 1105 68-69 0.6956426557096194 1105 70-71 0.5482181811502294 1105 72-73 0.5941748940833627 1105 74-75 0.48847640147793214 1105 76-77 0.6299860161256348 1105 78-79 0.7792767298440424 1105 80-81 0.850121686094127 1105 82-83 0.8447178719324562 1105 84-85 0.9131121604311438 1105 86-87 1.1492566317366375 1105 88-89 1.224849789879432 1105 90-91 1.0605049054599966 1105 92-93 0.9516871701466947 1105 94-95 1.0457100765841751 1105 96-97 0.9404316169712175 1105 98-99 0.980414159052291 1105 100-101 1.154766647780633 1106 1 0.021941990221947094 1106 2 0.014123094111248236 1106 3 0.038408977505227426 1106 4 0.0462467698609359 1106 5 0.0680965702218046 1106 6 0.09376120159353718 1106 7 0.08218494535828569 1106 8 0.04163221245782012 1106 9 0.12089357022627212 1106 10-11 0.09547645619136347 1106 12-13 0.10927573608494612 1106 14-15 0.07638518160066354 1106 16-17 -0.02947003924230529 1106 18-19 -0.0798877121668582 1106 20-21 0.04944535952612483 1106 22-23 -0.001311269765203349 1106 24-25 0.00835096324255602 1106 26-27 -0.03582936722700936 1106 28-29 0.07851716238625528 1106 30-31 0.24896585759605472 1106 32-33 0.28244783286666575 1106 34-35 0.16304442614669057 1106 36-37 0.24798450982592612 1106 38-39 0.11425098438463266 1106 40-41 0.130024389304765 1106 42-43 0.1989921425175325 1106 44-45 0.23749370601215958 1106 46-47 0.17496483067995428 1106 48-49 0.20298722783800116 1106 50-51 0.27126099523184166 1106 52-53 0.0592041640208123 1106 54-55 0.09311329067888607 1106 56-57 0.21172131120115978 1106 58-59 0.23382171820837527 1106 60-61 -0.08388119813401573 1106 62-63 -0.013634676017581171 1106 64-65 0.10110615445960747 1106 66-67 0.051133856303167136 1106 68-69 -0.1671664320917614 1106 70-71 0.09043308975609321 1106 72-73 -0.02221438394651898 1106 74-75 -0.04180398112400141 1106 76-77 0.03349096622248737 1106 78-79 0.013891044572343247 1106 80-81 -0.07710904137237407 1106 82-83 -0.05172640344977353 1106 84-85 0.13086363674642243 1106 86-87 0.11380914566192502 1106 88-89 0.28575826838656226 1106 90-91 0.3059067112231588 1106 92-93 0.19234080412775256 1106 94-95 0.09450723991391285 1106 96-97 0.1517390860205481 1106 98-99 0.5237425880919631 1106 100-101 0.12464210776763096 1107 1 -0.05797047785217302 1107 2 0.023460571804704955 1107 3 -0.013921750032650948 1107 4 -0.049137377803269544 1107 5 0.06321802710267121 1107 6 0.020706562227289282 1107 7 0.05034853252048066 1107 8 0.07826007984885308 1107 9 0.09120903668055291 1107 10-11 0.13140048962704043 1107 12-13 0.04223574976576572 1107 14-15 0.1515196542635664 1107 16-17 0.1733687313204939 1107 18-19 0.11573141628244343 1107 20-21 0.26451690674158357 1107 22-23 0.19782465331817178 1107 24-25 -0.042659184950515794 1107 26-27 0.1334312191798972 1107 28-29 0.10996009324901479 1107 30-31 0.2367592075131597 1107 32-33 0.18510181732690256 1107 34-35 0.24544841891992775 1107 36-37 0.2887340415269577 1107 38-39 0.2670486649786099 1107 40-41 0.1039920335273905 1107 42-43 -0.27532227254023667 1107 44-45 -0.10630345046699574 1107 46-47 -0.10565194637806741 1107 48-49 -0.14559663290896907 1107 50-51 -0.06167700879322524 1107 52-53 -0.12566586025313597 1107 54-55 -0.004254101145242828 1107 56-57 0.13527813490699003 1107 58-59 0.1643670034709359 1107 60-61 0.22135630821949093 1107 62-63 0.17264539130980694 1107 64-65 -0.016981190621713438 1107 66-67 -0.026144578509452288 1107 68-69 0.0275715184134242 1107 70-71 0.049375581217457665 1107 72-73 0.015715853361907506 1107 74-75 -0.1826412347623254 1107 76-77 -0.28810493966485495 1107 78-79 0.0474027247324571 1107 80-81 0.06160166782940024 1107 82-83 0.03037990477091057 1107 84-85 0.031208982495943616 1107 86-87 -0.0472437392655749 1107 88-89 -0.07172129351044987 1107 90-91 0.26834066246306065 1107 92-93 0.31231955451767135 1107 94-95 0.014387342710087125 1107 96-97 -0.18095111144882026 1107 98-99 0.030817346500366938 1107 100-101 0.06861961674962558 1108 1 -0.0021311489570479125 1108 2 -0.04243040350652194 1108 3 0.04199967576667518 1108 4 0.01493701691864402 1108 5 0.013541936706516822 1108 6 0.024630794248068355 1108 7 0.048246851772951516 1108 8 0.008995668110486577 1108 9 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-0.45761105982470696 2316 82-83 -0.4819424034302848 2316 84-85 -0.7165032391961113 2316 86-87 -0.978109614372471 2316 88-89 -1.126982953112087 2316 90-91 -1.3540687311431476 2316 92-93 -1.1515848359421206 2316 94-95 -0.995852914350909 2316 96-97 -1.4514748455640287 2316 98-99 -1.4193655605109576 2316 100-101 -0.9930847031751355 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 9.0 5 29.0 6 83.0 7 161.0 8 318.0 9 662.0 10 1101.0 11 1774.0 12 2633.0 13 3838.0 14 4358.0 15 6081.0 16 8531.0 17 12490.0 18 11955.0 19 7032.0 20 5819.0 21 5932.0 22 6060.0 23 6090.0 24 6955.0 25 7846.0 26 8651.0 27 10018.0 28 12031.0 29 14367.0 30 17245.0 31 21082.0 32 25734.0 33 33202.0 34 46577.0 35 74622.0 36 113650.0 37 184444.0 38 160427.0 39 14634.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.39693488888222 20.603185076327865 15.47899109258315 20.52088894220676 2 15.73627850313769 22.520243459506506 38.67256943987815 23.07090859747765 3 19.38829065459332 26.858973056143693 31.57740575269325 22.17533053656974 4 12.723760017120114 18.325217617936907 37.435186856725444 31.51583550821754 5 12.475924838871268 38.81483854847252 33.71921338333873 14.990023229317478 6 28.50188237572674 37.873351800421545 17.22627841944998 16.398487404401735 7 25.69081216532388 35.92474322816977 21.01828815591738 17.36615645058898 8 25.46593133064656 33.23776993770048 20.272630651461007 21.023668080191957 9 26.1720165032166 17.83504673958656 21.918767925608797 34.07416883158805 10-11 24.369144101869463 27.14691855870633 26.604861299574505 21.879076039849696 12-13 26.766617689430124 25.620992703627145 27.178839442735487 20.433550164207244 14-15 23.313987304430057 26.85401768341416 27.593705895328203 22.238289116827577 16-17 19.969811726799115 29.922975069268638 28.507234724285418 21.59997847964683 18-19 19.817249950086257 28.012428820613 32.22072514205989 19.94959608724085 20-21 21.225594571297744 26.52481998175608 31.765882433112598 20.48370301383358 22-23 20.6412750181423 26.52972169053958 31.912216373381092 20.916786917937028 24-25 21.111508961496916 27.07769061599793 31.969018679773015 19.841781742732138 26-27 20.749789284283896 26.570086139389193 32.84833848416195 19.831786092164965 28-29 19.731171161693027 27.538577045895536 32.896443630946756 19.833808161464678 30-31 20.113650764805325 27.29106838414102 32.11500893089852 20.48027192015514 32-33 20.10854296108641 27.60403279123622 31.7707841418961 20.516640105781267 34-35 21.142158533158767 26.981604701352897 31.454585672352053 20.421651093136287 36-37 21.23346236960341 26.54487747533781 31.96463999598182 20.257020159076966 38-39 20.736969552024632 26.84599632292492 31.965262103705477 20.451772021344972 40-41 20.193200439776714 27.189273178702038 32.312289296076266 20.30523708544498 42-43 20.242154026604304 27.693833519555078 30.93797769762969 21.126034756210927 44-45 20.335273743724752 27.404261729440076 30.898913346269403 21.36155118056577 46-47 21.146507323931537 27.80918085783013 30.000860795087732 21.043451023150606 48-49 21.115133458785948 27.564983895997074 29.92301880115442 21.396863844062562 50-51 20.587117113778223 28.191161860401724 30.095296391983673 21.126424633836375 52-53 21.029239905911044 28.36578724777854 29.515955968282338 21.089016878028076 54-55 20.25254560083592 27.855932801159195 29.685166831451753 22.20635476655313 56-57 20.882295625643348 28.42871540559249 28.833345487977063 21.855643480787094 58-59 21.188441266298145 27.526447965193956 29.179352384277262 22.105758384230633 60-61 20.661538939796838 28.04273955961969 29.92815363572016 21.36756786486331 62-63 20.640327301247225 28.03975533705716 29.852370112479914 21.467547249215702 64-65 20.327486470798863 28.337407187558096 29.677744083048047 21.657362258594993 66-67 20.36129172642066 28.064460785339346 29.06217065561315 22.512076832626846 68-69 20.139976937520288 28.118530863850673 29.446566243057493 22.29492595557155 70-71 20.755508743572484 28.23348393136173 28.81642861498034 22.194578710085445 72-73 20.67995069598287 27.599728851816565 29.148967712085582 22.571352740114985 74-75 20.134007935986077 28.966110063686344 28.46601621389622 22.433865786431355 76-77 20.546610908136802 27.912691295547166 29.000006589426608 22.540691206889424 78-79 20.27149570112877 28.041624000750804 29.248225068011415 22.438655230109013 80-81 20.54865845231235 28.5479100262786 28.61587810937541 22.28755341203364 82-83 20.432475471955268 28.183893534567012 28.74705373544203 22.63657725803569 84-85 20.063650481861885 27.932925495222026 28.66369854251874 23.339725480397348 86-87 20.413900735872733 27.887284667313356 28.57079595973136 23.12801863708255 88-89 20.476607165792217 27.570799078618695 28.380848808722153 23.571744946866932 90-91 20.100178044961904 27.426965260529528 28.81796027803098 23.654896416477587 92-93 20.331391379926668 27.78892285547252 28.728377187686426 23.15130857691439 94-95 19.932978098926046 27.303295024287372 28.818221923071864 23.945504953714718 96-97 19.74533829561608 27.07273538065355 28.800109511347454 24.38181681238291 98-99 19.862284494482232 27.40973740322763 28.13346412659927 24.594513975690866 100-101 19.916515131408268 26.626508328760373 29.005044002013296 24.45193253781806 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2810.0 1 1784.0 2 934.0 3 1109.5 4 949.5 5 919.0 6 1561.0 7 2959.5 8 4398.5 9 4726.0 10 3718.0 11 2835.0 12 2625.5 13 2561.0 14 2593.0 15 2529.5 16 2500.0 17 2507.5 18 2540.0 19 2661.0 20 2822.5 21 3083.0 22 3375.0 23 3827.0 24 4250.0 25 4885.5 26 6321.5 27 7785.0 28 8411.0 29 9139.5 30 10603.0 31 12383.5 32 15224.0 33 17405.0 34 18615.5 35 19557.5 36 20956.5 37 23051.0 38 23383.0 39 22575.0 40 22291.5 41 23234.5 42 23968.5 43 24192.5 44 24360.5 45 25163.5 46 25683.0 47 29032.0 48 43046.0 49 42942.0 50 29322.0 51 23672.0 52 20822.0 53 20628.0 54 21052.5 55 20499.5 56 18548.5 57 16489.0 58 15525.0 59 14043.5 60 11949.5 61 10542.5 62 9438.5 63 7533.5 64 5588.0 65 4434.5 66 3471.5 67 3119.5 68 2502.5 69 1586.5 70 1282.0 71 1149.0 72 993.5 73 847.5 74 647.5 75 536.0 76 371.0 77 194.5 78 119.0 79 72.0 80 37.0 81 17.5 82 9.0 83 5.0 84 4.5 85 2.0 86 1.5 87 0.5 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34288050709970674 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011357617912995865 16-17 0.003048623755593627 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.184385546893213E-4 26-27 9.564309821470202E-4 28-29 0.0 30-31 0.002510631328135928 32-33 0.0 34-35 0.010520740803617222 36-37 0.029171144955484115 38-39 0.021579474034692143 40-41 0.013091149068137338 42-43 0.008906763521244126 44-45 0.0026301852009043055 46-47 8.368771093786426E-4 48-49 5.977693638418876E-4 50-51 0.0 52-53 5.977693638418876E-5 54-55 0.0 56-57 0.0 58-59 0.00436371635604578 60-61 0.008966540457628314 62-63 0.0032279545647461933 64-65 0.0013748695368363416 66-67 0.0032877315011303814 68-69 0.0023313005189833616 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.21190923948194917 78-79 2.988846819209438E-4 80-81 0.002510631328135928 82-83 2.988846819209438E-4 84-85 0.0 86-87 0.10813647791899747 88-89 0.4000272582829912 90-91 0.3857405704871701 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0010162079185312089 100-101 4.782154910735101E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 836443.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.6295839922053 #Duplication Level Percentage of deduplicated Percentage of total 1 66.61332280764242 22.401783343594957 2 14.376501888451578 9.669515555435607 3 6.378990537025666 6.435683941511622 4 3.4335148011903405 4.618706975804424 5 2.1446895181543377 3.6062508143986802 6 1.3909798208036048 2.8066843629106497 7 0.9646461241807955 2.2708453499125354 8 0.7008297074042105 1.8854889207506047 9 0.5175621894387696 1.5664861008828694 >10 3.0106824092961015 19.20588004138935 >50 0.2968974869236944 6.869960411525894 >100 0.15502668327182828 9.413206380433877 >500 0.007466881533734232 1.7437665283638715 >1k 0.007466881533734232 4.296340352747302 >5k 0.0014222631492827108 3.2094009203378118 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9166 1.0958307977949484 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7436 0.8890025979056552 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5136 0.6140286905383869 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5102 0.6099638588642621 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3798 0.45406560877429786 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3235 0.38675677840570133 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2703 0.32315411809292444 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2614 0.3125138234165388 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2069 0.24735696275777308 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1841 0.22009867976658304 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1721 0.20575221503437774 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1672 0.1998940752687272 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1658 0.19822032104996995 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1467 0.17538553135120982 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1393 0.16653854476634988 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1313 0.15697423494487966 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1286 0.1537462803801335 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1280 0.15302895714352321 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1252 0.14968144870600866 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1173 0.14023669275730685 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1167 0.13951936952069657 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1140 0.13629141495595037 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1084 0.12959639808092122 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1040 0.12433602767911263 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1024 0.12242316571481858 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 991 0.11847788791346213 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 888 0.10616383901831923 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 870 0.10401186930848845 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 860 0.10281633058080468 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.7933080915256627E-4 0.0 14-15 0.0 0.0 0.0 2.988846819209438E-4 0.0 16-17 0.0 0.0 0.0 5.379924274576988E-4 0.0 18-19 0.0 0.0 0.0 5.977693638418877E-4 0.0 20-21 0.0 0.0 0.0 0.001255315664067964 0.0 22-23 0.0 0.0 0.0 0.002510631328135928 0.0 24-25 0.0 0.0 0.0 0.006216801383955631 0.0 26-27 0.0 0.0 0.0 0.01016207918531209 0.0 28-29 0.0 0.0 0.0 0.022775012762375917 0.0 30-31 0.0 0.0 0.0 0.05116905754486558 0.0 32-33 0.0 0.0 0.0 0.09922971439775334 0.0 34-35 0.0 0.0 0.0 0.17329333857776322 0.0 36-37 0.0 0.0 0.0 0.2817884781150658 0.0 38-39 0.0 0.0 0.0 0.42573134092819237 0.0 40-41 0.0 0.0 0.0 0.5786407441989472 0.0 42-43 0.0 0.0 0.0 0.7249746844674413 0.0 44-45 0.0 0.0 0.0 0.8989255693454306 0.0 46-47 0.0 0.0 0.0 1.1178287103843298 0.0 48-49 0.0 0.0 0.0 1.3422911065069587 0.0 50-51 0.0 0.0 0.0 1.577035135687668 0.0 52-53 0.0 0.0 0.0 1.8062796867210318 0.0 54-55 0.0 0.0 0.0 2.0602718894174497 0.0 56-57 0.0 0.0 0.0 2.376133221271503 0.0 58-59 0.0 0.0 0.0 2.7023957400564056 0.0 60-61 0.0 0.0 0.0 3.070322783501087 0.0 62-63 0.0 0.0 0.0 3.4280877477604568 0.0 64-65 0.0 0.0 0.0 3.7992427457698854 0.0 66-67 0.0 0.0 0.0 4.240217205476045 0.0 68-69 0.0 0.0 0.0 4.736126669719275 0.0 70-71 0.0 0.0 0.0 5.284221399425903 0.0 72-73 0.0 0.0 0.0 5.8187467645733175 0.0 74-75 0.0 0.0 0.0 6.392964015479835 0.0 76-77 0.0 0.0 0.0 6.9926462412860175 0.0 78-79 0.0 0.0 0.0 7.681635210050176 0.0 80-81 0.0 0.0 0.0 8.412049595728579 0.0 82-83 0.0 0.0 0.0 9.1624294781593 0.0 84-85 0.0 0.0 0.0 9.958777824669465 0.0 86-87 0.0 0.0 0.0 10.787764378445392 0.0 88-89 0.0 0.0 0.0 11.633787359090817 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTATC 35 0.008436522 27.113855 92-93 AGGTAAG 265 0.0 26.858065 2 GGTAAGG 265 0.0 26.858065 3 TATAGGG 165 8.491952E-8 25.881409 2 GTATAGG 205 9.276846E-10 25.553755 1 GAGTACT 6410 0.0 25.42717 12-13 GTATAGT 210 1.2259989E-9 24.945333 1 GTACTTT 6605 0.0 24.460968 14-15 AGTACTT 6540 0.0 23.724623 12-13 TAAGGTG 360 0.0 22.406588 5 CGGTATA 75 3.04674E-5 22.154902 34-35 GTACATG 5430 0.0 21.750425 1 ATAGGTG 285 8.54925E-11 21.643517 4 AAGGTAA 335 1.8189894E-12 21.32369 1 ACTTTTT 7675 0.0 21.236242 16-17 TACATGG 5525 0.0 20.869081 2 TAATACC 275 1.2751116E-9 20.705128 4 ACATGGG 5455 0.0 20.701963 3 GTAAGGT 345 1.8189894E-12 20.630108 4 GGTGTGC 700 0.0 20.335392 8 >>END_MODULE