##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939082_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 821396 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.225318847425605 33.0 31.0 34.0 31.0 34.0 2 32.29855270782911 34.0 31.0 34.0 31.0 34.0 3 32.33123243843408 34.0 31.0 34.0 30.0 34.0 4 35.79039099289502 37.0 35.0 37.0 35.0 37.0 5 35.846310427613474 37.0 35.0 37.0 35.0 37.0 6 35.959428826047365 37.0 37.0 37.0 35.0 37.0 7 35.90773147178705 37.0 37.0 37.0 35.0 37.0 8 35.91516150553448 37.0 37.0 37.0 35.0 37.0 9 37.59438443820033 39.0 38.0 39.0 35.0 39.0 10-11 37.51411195087388 39.0 38.0 39.0 35.0 39.0 12-13 37.433552756526694 39.0 38.0 39.0 35.0 39.0 14-15 38.68958090859951 40.0 38.0 41.0 35.0 41.0 16-17 38.629275039079815 40.0 38.0 41.0 35.0 41.0 18-19 38.598691130709184 40.0 38.0 41.0 35.0 41.0 20-21 38.597537606708585 40.0 38.0 41.0 34.5 41.0 22-23 38.61140302606782 40.0 38.0 41.0 35.0 41.0 24-25 38.537451485032804 40.5 38.0 41.0 34.5 41.0 26-27 38.39356412741236 40.0 38.0 41.0 34.0 41.0 28-29 38.241470618313215 40.0 38.0 41.0 34.0 41.0 30-31 37.962910703241796 40.0 37.5 41.0 34.0 41.0 32-33 37.670778163029766 40.0 37.0 41.0 33.0 41.0 34-35 37.314576038841196 40.0 36.0 41.0 33.0 41.0 36-37 36.99908083311825 40.0 35.5 41.0 32.0 41.0 38-39 36.75492393437514 40.0 35.0 41.0 31.0 41.0 40-41 36.526635751817636 39.5 35.0 41.0 31.0 41.0 42-43 36.250935602316055 39.0 35.0 41.0 30.0 41.0 44-45 35.864385144315285 39.0 35.0 41.0 29.5 41.0 46-47 35.47135242927894 39.0 35.0 41.0 26.5 41.0 48-49 35.202178973357555 39.0 35.0 41.0 24.5 41.0 50-51 33.99515824279641 38.0 34.0 40.0 23.0 40.5 52-53 34.09220643879444 38.0 34.5 39.5 22.5 40.5 54-55 34.560445875071224 39.0 35.0 40.5 22.5 41.0 56-57 34.44005571003511 39.0 35.0 41.0 21.5 41.0 58-59 34.226793775474924 38.0 35.0 41.0 20.0 41.0 60-61 33.901114687678046 38.0 34.5 40.0 18.0 41.0 62-63 33.51736434070777 37.0 34.0 40.0 14.0 41.0 64-65 33.12793585554349 37.0 34.0 40.0 9.5 41.0 66-67 32.70226236796867 36.0 33.0 39.0 6.5 41.0 68-69 32.27265898543455 35.5 33.0 39.0 2.0 41.0 70-71 31.805002702715864 35.0 33.0 38.5 2.0 40.0 72-73 31.348108585870882 35.0 32.0 37.5 2.0 40.0 74-75 30.901512181700422 35.0 32.0 37.0 2.0 39.0 76-77 30.455126394577036 35.0 31.0 36.5 2.0 39.0 78-79 30.00280011103049 35.0 31.0 36.0 2.0 38.5 80-81 29.55926374124052 35.0 30.5 35.5 2.0 37.0 82-83 29.166137892076417 34.5 30.0 35.0 2.0 37.0 84-85 28.77637887206658 34.0 29.5 35.0 2.0 36.5 86-87 28.37345141685618 34.0 29.0 35.0 2.0 36.0 88-89 27.913443698289257 34.0 28.5 35.0 2.0 36.0 90-91 27.48200563431037 34.0 27.0 35.0 2.0 35.0 92-93 27.078453023876424 34.0 26.5 35.0 2.0 35.0 94-95 26.5900199173115 34.0 25.0 35.0 2.0 35.0 96-97 25.98132143326727 33.5 23.5 35.0 2.0 35.0 98-99 25.30913104032647 33.0 13.5 35.0 2.0 35.0 100-101 22.563591738941994 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24875876094752414 1101 2 0.4193198196831318 1101 3 0.2705181315238576 1101 4 0.25821560581616154 1101 5 0.06185924736799109 1101 6 0.0623665615234259 1101 7 0.111577535505063 1101 8 0.2508376882231218 1101 9 0.13923214420891128 1101 10-11 0.2141985850621495 1101 12-13 0.3466326233945196 1101 14-15 0.34703038416176213 1101 16-17 0.30613798413398285 1101 18-19 0.3512378445796216 1101 20-21 0.4218470886790726 1101 22-23 0.336728192766536 1101 24-25 0.33630358852399667 1101 26-27 0.24071519638869887 1101 28-29 0.3708244174052737 1101 30-31 0.368813459003853 1101 32-33 0.32214634359834093 1101 34-35 0.349471503298453 1101 36-37 0.24328754102329953 1101 38-39 0.3522301513959931 1101 40-41 0.3300536103900811 1101 42-43 0.594281562861319 1101 44-45 0.6996350797453204 1101 46-47 0.5759639183045451 1101 48-49 0.6238167087309492 1101 50-51 0.5662023819431639 1101 52-53 0.31874165025161716 1101 54-55 0.424488120872482 1101 56-57 0.4854250471383068 1101 58-59 0.5709030868705227 1101 60-61 0.27910956032332024 1101 62-63 -0.09233980277551979 1101 64-65 0.06263501239767066 1101 66-67 0.06268122404288334 1101 68-69 0.024161996796451035 1101 70-71 -0.11660379519736352 1101 72-73 -0.20463662748938205 1101 74-75 -0.1810047829435888 1101 76-77 -0.009673960653255875 1101 78-79 -0.06899366574511845 1101 80-81 -0.16283487292491117 1101 82-83 -0.6539497136717856 1101 84-85 -0.8452235195946116 1101 86-87 -0.9230351411080342 1101 88-89 -0.7930296340267908 1101 90-91 -0.8514509254972715 1101 92-93 -0.7669470448096156 1101 94-95 -0.886577670235166 1101 96-97 -0.8293903137239838 1101 98-99 -0.9810793020097037 1101 100-101 -1.4622535512059116 1102 1 0.20493943141391213 1102 2 0.09285487076920873 1102 3 0.227438608852502 1102 4 -0.016611699623389597 1102 5 0.05932464279256777 1102 6 0.11644688090986932 1102 7 0.027217665430910642 1102 8 0.03231887786757426 1102 9 -0.012437017664332473 1102 10-11 0.2147085702660192 1102 12-13 0.24195870749611714 1102 14-15 0.26811400422496945 1102 16-17 0.25828911485653805 1102 18-19 0.1934685592593226 1102 20-21 0.3353290837937237 1102 22-23 0.2954675443630279 1102 24-25 0.26286571916775614 1102 26-27 0.3836839669084995 1102 28-29 0.460535848953441 1102 30-31 0.30327051080784173 1102 32-33 0.3630348997930213 1102 34-35 0.25541315924011343 1102 36-37 0.4757138448781717 1102 38-39 0.39697095634087276 1102 40-41 0.42930592392811207 1102 42-43 0.5048831265241134 1102 44-45 0.5004125335840044 1102 46-47 0.7022082384264152 1102 48-49 0.7925167498799652 1102 50-51 0.5523517966640057 1102 52-53 0.7629781608146615 1102 54-55 0.4942182008066496 1102 56-57 0.49346661640436906 1102 58-59 0.538401926818338 1102 60-61 0.31308376776691205 1102 62-63 0.503493639996698 1102 64-65 0.6578337096371811 1102 66-67 0.4714858012148042 1102 68-69 0.30346097507502634 1102 70-71 0.2770128535213239 1102 72-73 0.3100238055383997 1102 74-75 0.31149646465970093 1102 76-77 0.3228714127084409 1102 78-79 0.23368364713627443 1102 80-81 0.46051369430120914 1102 82-83 0.06062710804789262 1102 84-85 0.0890934504366463 1102 86-87 0.09139109270595469 1102 88-89 0.0850357491406335 1102 90-91 -0.0393990777311366 1102 92-93 0.006411590793916844 1102 94-95 -0.2161966478133266 1102 96-97 -0.11235330587064851 1102 98-99 -0.4343970512458206 1102 100-101 -0.6265782257346686 1103 1 0.27908909127785364 1103 2 0.22901888044602714 1103 3 0.30672029113027577 1103 4 0.22135534129632362 1103 5 0.2752769203601346 1103 6 0.20597399874788636 1103 7 0.24817583593315362 1103 8 0.27408220933997995 1103 9 0.2976181898289951 1103 10-11 0.3084641272000326 1103 12-13 0.3377002868026082 1103 14-15 0.4148439424008501 1103 16-17 0.556233824655358 1103 18-19 0.5236166063200827 1103 20-21 0.47981143600512155 1103 22-23 0.5167007184837402 1103 24-25 0.5521364426547208 1103 26-27 0.5773454436572507 1103 28-29 0.617961980500283 1103 30-31 0.6510094848169032 1103 32-33 0.6343755941254656 1103 34-35 0.48656817443767864 1103 36-37 0.5158643731746011 1103 38-39 0.6324071368921196 1103 40-41 0.5670320782196114 1103 42-43 0.5655038489772224 1103 44-45 0.6652379166247613 1103 46-47 0.7189100559315307 1103 48-49 0.7086323754615051 1103 50-51 0.4833258884283751 1103 52-53 0.5484298490887554 1103 54-55 0.44956319594756167 1103 56-57 0.7194069013329312 1103 58-59 0.5938792545275433 1103 60-61 0.5816589181918346 1103 62-63 0.5536125325385157 1103 64-65 0.5694396894151126 1103 66-67 0.3628697104015828 1103 68-69 0.18727298012019844 1103 70-71 0.21636214947070798 1103 72-73 0.24183324664006633 1103 74-75 0.11036957013289594 1103 76-77 0.1962777357239709 1103 78-79 0.32560412353351964 1103 80-81 0.20472311059052117 1103 82-83 -0.16187253010524216 1103 84-85 -0.184498965253038 1103 86-87 0.08933787020114536 1103 88-89 0.05847825684280039 1103 90-91 0.17302674366125004 1103 92-93 -0.17922102091337777 1103 94-95 -0.4157355515207044 1103 96-97 -0.16782236279432894 1103 98-99 -0.3508000866241048 1103 100-101 -0.6180934336415405 1104 1 0.11597433717949457 1104 2 0.1797687932468648 1104 3 0.18797247110934734 1104 4 0.1379231822450464 1104 5 0.16317367655127413 1104 6 0.07469856565870714 1104 7 0.23333453840960772 1104 8 -3.6843593382229756E-4 1104 9 0.20528590288562043 1104 10-11 0.280461569357918 1104 12-13 0.3079566523414954 1104 14-15 0.4564553829310185 1104 16-17 0.3453862487920816 1104 18-19 0.19671857120788871 1104 20-21 0.10837788176724672 1104 22-23 0.21055899077766327 1104 24-25 0.1421114455613619 1104 26-27 0.2034238066382983 1104 28-29 0.22360957961666372 1104 30-31 0.24773951620860402 1104 32-33 0.376809613425543 1104 34-35 0.23160363543058082 1104 36-37 0.11031850641492014 1104 38-39 -0.010837834622812181 1104 40-41 -0.007531925965949426 1104 42-43 0.10974579758785552 1104 44-45 0.3082416371223715 1104 46-47 0.4269091258071285 1104 48-49 0.4409658250603883 1104 50-51 0.3524661046822999 1104 52-53 0.3332397549136559 1104 54-55 0.4831697533246171 1104 56-57 0.3979995068439166 1104 58-59 0.11103132986761466 1104 60-61 0.20986203410858195 1104 62-63 0.23381832256293222 1104 64-65 0.18532737689331924 1104 66-67 -0.009310849998371395 1104 68-69 -0.16979015207373038 1104 70-71 -0.08058006655068439 1104 72-73 -0.22951601507368835 1104 74-75 -0.1518731923365877 1104 76-77 -0.2518649221065168 1104 78-79 -0.11216183391328727 1104 80-81 -0.21066754165921964 1104 82-83 -0.22839095819499988 1104 84-85 -0.2697361469760935 1104 86-87 -0.07042843604231308 1104 88-89 -0.18727223728578934 1104 90-91 -0.2640561536762682 1104 92-93 -0.39634693650458885 1104 94-95 -0.3175086011660966 1104 96-97 -0.02627777495570527 1104 98-99 -0.1296048767647875 1104 100-101 -0.18930027005590944 1105 1 0.21206303557255524 1105 2 0.2766933657359232 1105 3 0.21701449311456145 1105 4 0.08729940659807767 1105 5 0.192307755174987 1105 6 0.14804927928161504 1105 7 0.08959733427958128 1105 8 0.047556239658653965 1105 9 0.1861712666197306 1105 10-11 0.1911924252635444 1105 12-13 0.3520720250603375 1105 14-15 0.33864868710082163 1105 16-17 0.32442311562665793 1105 18-19 0.339818743537883 1105 20-21 0.26533073404951324 1105 22-23 0.1840890306934071 1105 24-25 0.2644197100363428 1105 26-27 0.41253593896416163 1105 28-29 0.21618797503298026 1105 30-31 0.6210272631044518 1105 32-33 0.4485456539599184 1105 34-35 0.2830411915851201 1105 36-37 0.3718439950514494 1105 38-39 0.5871766882293201 1105 40-41 0.4162138946635707 1105 42-43 0.4509610185973756 1105 44-45 0.4822047113715584 1105 46-47 0.7062788693742164 1105 48-49 0.7778157212205272 1105 50-51 0.6413968486470623 1105 52-53 0.6600297038038221 1105 54-55 0.47755061733121096 1105 56-57 0.2479085549048392 1105 58-59 0.5890647795605162 1105 60-61 0.6880906438327088 1105 62-63 0.6686017317509183 1105 64-65 0.39749210668872337 1105 66-67 0.3076566576975637 1105 68-69 0.5323514530991176 1105 70-71 0.5159836439938523 1105 72-73 0.4656016701074037 1105 74-75 0.4109793845122809 1105 76-77 0.31145714349671394 1105 78-79 0.24194867821609378 1105 80-81 0.33453041689320884 1105 82-83 0.24545575648840057 1105 84-85 0.2908647249841998 1105 86-87 0.30581432830747346 1105 88-89 0.4796669169716452 1105 90-91 0.4231538679754472 1105 92-93 0.4396449919711003 1105 94-95 0.06591457942689516 1105 96-97 0.4314884896076485 1105 98-99 0.4478847024259984 1105 100-101 -0.017592582960038072 1106 1 0.0314700436588069 1106 2 0.1892653877938386 1106 3 0.23004646719898147 1106 4 0.29566262032739843 1106 5 0.3044767009946199 1106 6 0.16804235527261824 1106 7 0.235792838619453 1106 8 0.19587840390603617 1106 9 0.3529038762292629 1106 10-11 0.32235293862935066 1106 12-13 0.24691363835443525 1106 14-15 0.17280012506911646 1106 16-17 0.24686803266219925 1106 18-19 0.2487618430210503 1106 20-21 0.24025593002369305 1106 22-23 0.34714326052105804 1106 24-25 0.22144757917256896 1106 26-27 0.3401862270523921 1106 28-29 0.37299205030685556 1106 30-31 0.39673882867234056 1106 32-33 0.22491870967346017 1106 34-35 0.2177329495415492 1106 36-37 0.18569324948405352 1106 38-39 0.31406780818777946 1106 40-41 0.3439754614678989 1106 42-43 0.3858444232315179 1106 44-45 0.19493870578289574 1106 46-47 0.4910720146053009 1106 48-49 0.5329345421607101 1106 50-51 0.49964631079993893 1106 52-53 0.34894387082081124 1106 54-55 0.3585627590755678 1106 56-57 0.31813160690909115 1106 58-59 0.4082430480636887 1106 60-61 0.5170985322572506 1106 62-63 0.40169354905599874 1106 64-65 0.4008861075295016 1106 66-67 0.37717494449990596 1106 68-69 0.5141759294709374 1106 70-71 0.5048928652901203 1106 72-73 0.33039184790606413 1106 74-75 -0.004856813217635647 1106 76-77 0.2995812316294568 1106 78-79 0.481383382338354 1106 80-81 0.29492137611574165 1106 82-83 0.3179291114180458 1106 84-85 0.3873990111930574 1106 86-87 0.28439935761086943 1106 88-89 0.507869889349795 1106 90-91 0.682235447638643 1106 92-93 0.264529385749384 1106 94-95 0.46918864654032433 1106 96-97 0.4737929481748715 1106 98-99 0.724709055154797 1106 100-101 0.42388717667793685 1107 1 0.1434685914896292 1107 2 0.2535288699023468 1107 3 0.36843052930919384 1107 4 0.3684242897224266 1107 5 0.3320379394018289 1107 6 0.3642084288036713 1107 7 0.15316741000471978 1107 8 0.3694925565603242 1107 9 0.27770416133403586 1107 10-11 0.35059196700826334 1107 12-13 0.4499958341274848 1107 14-15 0.45071316478227885 1107 16-17 0.4136193536865065 1107 18-19 0.40541209506899634 1107 20-21 0.4362363190784748 1107 22-23 0.42209151034078474 1107 24-25 0.556520628944341 1107 26-27 0.4088070493598437 1107 28-29 0.3525326186179214 1107 30-31 0.31589363956560845 1107 32-33 0.4895857016349012 1107 34-35 0.6273046095940771 1107 36-37 0.5811387340206124 1107 38-39 0.7385233696460105 1107 40-41 0.9223368084547303 1107 42-43 0.7158440402052477 1107 44-45 0.6791988239215243 1107 46-47 0.7520144923856122 1107 48-49 0.6060667478424833 1107 50-51 0.5464004961946429 1107 52-53 0.4077375712043363 1107 54-55 0.5369414255113014 1107 56-57 0.5555329568269087 1107 58-59 0.6000459527351154 1107 60-61 0.5486815465407986 1107 62-63 0.42740293869530177 1107 64-65 0.5336047114486959 1107 66-67 0.5129819059191689 1107 68-69 0.38985392760909576 1107 70-71 0.6367979547101079 1107 72-73 0.6761704820343724 1107 74-75 0.6645199031147726 1107 76-77 0.6304951156657559 1107 78-79 0.6811990301273703 1107 80-81 0.6628430922066784 1107 82-83 0.6207430222661827 1107 84-85 0.4778015385003336 1107 86-87 0.4040909048871377 1107 88-89 0.4890666299627391 1107 90-91 0.5301040729573465 1107 92-93 0.5073164181450807 1107 94-95 0.7660365068389865 1107 96-97 0.6225711776275951 1107 98-99 0.7620532841978473 1107 100-101 0.8300632255454232 1108 1 -0.03021420450854606 1108 2 0.01825542612812825 1108 3 8.379381891003845E-4 1108 4 0.02269034338226561 1108 5 0.11741292369763556 1108 6 0.1362201397574836 1108 7 0.1356184725622711 1108 8 0.22460369452980444 1108 9 0.20425148144351368 1108 10-11 0.10750459445076643 1108 12-13 0.16273157591399467 1108 14-15 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-0.9420507257082207 2316 96-97 -0.9462846806183514 2316 98-99 -1.454328650259054 2316 100-101 -1.5691942831466434 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3043.0 3 596.0 4 562.0 5 566.0 6 671.0 7 811.0 8 1017.0 9 1329.0 10 1648.0 11 2297.0 12 3450.0 13 5963.0 14 5542.0 15 6096.0 16 8290.0 17 12761.0 18 10120.0 19 6107.0 20 5752.0 21 5770.0 22 5881.0 23 6302.0 24 6952.0 25 7657.0 26 8362.0 27 10024.0 28 11712.0 29 13663.0 30 16313.0 31 19531.0 32 24301.0 33 31602.0 34 44076.0 35 71249.0 36 107692.0 37 165583.0 38 160812.0 39 27290.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.97219373943608 20.11737487977141 15.16157378155122 20.74885759924129 2 15.630591539132851 22.230618841689054 38.97074238575587 23.16804723342223 3 19.35216898455688 26.87212803042307 31.334544080543132 22.44115890447692 4 12.606863394053319 17.93220475349344 37.77670502960419 31.68422682284905 5 12.601149304785194 38.55412874456616 33.77442028861837 15.070301662030275 6 28.606743325267853 37.3099068936697 17.70628498188313 16.37706479917932 7 25.72922060911046 35.7179498114426 21.481043736787274 17.071785842659665 8 25.36665484793434 32.90444737678971 20.695818727451503 21.03307904782444 9 26.395436252404874 17.779315705915106 22.154816023315856 33.67043201836416 10-11 24.271835788316842 26.861554525733006 27.008920548452597 21.857689137497555 12-13 26.664680588707622 25.61766415299867 27.34159200597529 20.37606325231841 14-15 23.404266984418236 26.676693872926176 27.704086543440464 22.214952599215124 16-17 20.066317311827824 29.817082160119206 28.62159168858765 21.495008839465317 18-19 19.898072019764157 28.082041971419958 32.07867889639962 19.941207112416265 20-21 21.37144235760292 26.67412373369985 31.477900518826658 20.476533389870575 22-23 20.738892544661354 26.697192366747093 31.59123589481124 20.972679193780312 24-25 21.114873614223114 27.156565290329894 31.695496838298965 20.033064257148027 26-27 20.852825842542572 26.632541445385932 32.48550439819258 20.02912831387891 28-29 19.870795711714305 27.603615087726567 32.48149741457369 20.04409178598544 30-31 20.167031938597756 27.307351805917833 31.927874178290477 20.59774207719393 32-33 20.071017872059375 27.72889646293709 31.463854059978907 20.736231605024624 34-35 21.195069618641195 27.10433237625643 31.147081404062966 20.55351660103941 36-37 21.317071917053802 26.63986890349868 31.604573691605026 20.438485487842488 38-39 20.820350617450117 27.139907436014305 31.515069943011664 20.524672003523918 40-41 20.293446835161998 27.398911669309918 31.97364373041644 20.333997765111643 42-43 20.36520299557736 27.811443320854817 30.605007026428794 21.21834665713903 44-45 20.43325695300621 27.52775542794908 30.66603849201372 21.37294912703099 46-47 21.13898338268765 28.012040821321065 29.69922419911407 21.149751596877216 48-49 21.049440164374275 27.764653045090952 29.70674635735129 21.479160433183488 50-51 20.660483455484183 28.30055105554733 29.914924757605316 21.124040731363174 52-53 20.993177715911525 28.51947257319424 29.333007005843303 21.154342705050937 54-55 20.289473780816856 27.932029461323754 29.669838853558524 22.10865790430087 56-57 20.935475781245223 28.50405837701619 28.800041593012377 21.760424248726213 58-59 21.26573267043943 27.479353880271773 29.220214879884626 22.034698569404174 60-61 20.740416073707838 27.88031278883819 30.023278218727228 21.35599291872674 62-63 20.78503870197734 27.851475923901507 29.953427937487113 21.410057436634037 64-65 20.44128643476835 28.124391912481904 29.84802554365319 21.586296109096562 66-67 20.472672240067904 27.949213957043483 29.296829819512126 22.281283983376486 68-69 20.2852592587152 28.021461465578657 29.69068656614102 22.002592709565118 70-71 20.83872685376445 28.27226797151609 28.97169664086957 21.917308533849887 72-73 20.8579439892884 27.663519603259502 29.31597267268664 22.162563734765463 74-75 20.351455882892918 28.958667368395286 28.735052568449138 21.95482418026266 76-77 20.83767474500643 27.91478249909909 29.062567109607922 22.184975646286556 78-79 20.691053578703087 28.11918530326033 29.250409513684914 21.93935160435167 80-81 21.03348503755903 28.658090484230105 28.59245393819276 21.715970540018105 82-83 20.858541766210532 28.31379367515856 28.831554340398117 21.996110218232786 84-85 20.65565426558231 28.012127426066378 28.72396524743644 22.60825306091487 86-87 21.105030848242553 28.051987776938304 28.632369089969195 22.210612284849947 88-89 21.187542483684123 27.614409976193844 28.47824743598921 22.719800104132826 90-91 20.900926591121323 27.601302686025008 28.8787158188723 22.619054903981368 92-93 21.311675054833152 27.791350070499764 28.855455898480336 22.041518976186747 94-95 21.047890633605444 27.49880671178724 28.825743204376614 22.627559450230702 96-97 20.957662425402546 27.13321795919966 28.78237860013806 23.12674101525974 98-99 21.290919538752735 27.457589571205034 28.11293863071273 23.138552259329497 100-101 21.75744761037734 26.4873195454957 28.847575136862762 22.90765770726419 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3515.0 1 2151.5 2 946.0 3 1128.5 4 972.5 5 978.0 6 1610.5 7 3004.0 8 4488.0 9 4801.0 10 3820.5 11 2909.0 12 2718.5 13 2671.5 14 2730.5 15 2778.0 16 2764.0 17 2709.5 18 2657.0 19 2740.0 20 2883.0 21 3102.5 22 3488.5 23 3904.5 24 4323.5 25 5063.0 26 6388.0 27 7798.0 28 8418.0 29 8916.0 30 10239.0 31 11978.5 32 14594.5 33 16780.0 34 18168.0 35 19268.5 36 20634.0 37 22798.0 38 23014.5 39 21970.5 40 21714.0 41 22701.0 42 23409.5 43 23517.5 44 23517.5 45 24151.5 46 25028.5 47 28464.5 48 42402.0 49 42003.5 50 27616.0 51 22154.0 52 19901.0 53 20057.0 54 20534.0 55 19913.5 56 18122.5 57 16135.0 58 15123.5 59 13753.0 60 11862.0 61 10467.0 62 9389.5 63 7519.0 64 5523.5 65 4445.5 66 3509.0 67 3088.0 68 2547.0 69 1735.5 70 1381.0 71 1202.0 72 1065.5 73 894.5 74 695.0 75 554.0 76 418.0 77 274.5 78 193.0 79 131.0 80 67.0 81 45.0 82 32.5 83 19.5 84 13.5 85 8.5 86 6.0 87 4.0 88 3.5 89 3.0 90 3.0 91 2.5 92 1.5 93 1.5 94 1.5 95 1.5 96 1.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38507613867123774 2 0.11687419953347715 3 0.11760466328056139 4 0.13063126677023018 5 0.13075301072807757 6 0.07475079011828643 7 0.08412507487253407 8 0.0809597319685024 9 0.08120321988419715 10-11 0.07919444457971551 12-13 0.0831511232097551 14-15 0.07657694948599701 16-17 0.07852485281155497 18-19 0.08747303371333681 20-21 0.08497728257746569 22-23 0.0944733112895607 24-25 0.1062216032218321 26-27 0.10816950654739005 28-29 0.10165620480255566 30-31 0.09429069535278964 32-33 0.09222104806938432 34-35 0.33570896376412834 36-37 0.5220380912495313 38-39 0.5020112101836386 40-41 0.47547102737291147 42-43 0.502132954141486 44-45 0.5129681663899021 46-47 0.5084027679706257 48-49 0.5157682774203917 50-51 0.5158291493993153 52-53 0.5138812460737574 54-55 0.5099854394226414 56-57 0.4810103774549669 58-59 0.4710882448904061 60-61 0.4217210699832967 62-63 0.4943413408392542 64-65 0.5216728593759892 66-67 0.5308645281934657 68-69 0.5466303707347004 70-71 0.5409692766947977 72-73 0.5281252891418998 74-75 0.5315341199616264 76-77 0.5076114322446177 78-79 0.4824713049491354 80-81 0.4889237347150461 82-83 0.5023764420571808 84-85 0.496106628228041 86-87 0.49775017165898056 88-89 0.507733176202465 90-91 0.5253251781114103 92-93 0.5322645837087105 94-95 0.5266643616477314 96-97 0.5288557528889841 98-99 0.529647088614992 100-101 0.5057244008979834 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 821396.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.29240890345049 #Duplication Level Percentage of deduplicated Percentage of total 1 68.27210824724072 24.094871609622544 2 13.815514569374155 9.751655787878608 3 6.0699720599553375 6.426718079173903 4 3.260610935656253 4.6029925766497115 5 2.0093770557494466 3.5457878346360445 6 1.3247080128027098 2.8051282119306338 7 0.8992744240439766 2.2216292482842466 8 0.6424621787270334 1.8139230333308922 9 0.4702431526938467 1.4936412266027048 >10 2.8087272975078026 18.85001109143728 >50 0.2734887911816692 6.742055685277109 >100 0.13830024780819428 8.842937140589543 >500 0.006569261770888376 1.5357905704708448 >1k 0.00795226424897014 5.366115217465904 >5k 6.915012390408817E-4 1.9067426866500414 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8775 1.0683032301106896 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6851 0.8340678552123458 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4703 0.5725618337561906 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4438 0.540299684926637 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3825 0.4656706387661981 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3051 0.3714408153923321 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2903 0.35342270963092104 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2494 0.30362943087134586 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2051 0.24969685754496004 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1896 0.23082654407861738 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1743 0.21219971852796943 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1495 0.18200721698182118 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1460 0.17774617845716317 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1457 0.17738094658362105 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1244 0.1514494835621308 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1220 0.14852762857379387 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1210 0.14731018899532017 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1168 0.14219694276573053 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1148 0.1397620636087831 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1133 0.13793590424107252 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1105 0.1345270734213461 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1088 0.13245742613794076 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1078 0.13123998655946706 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1033 0.12576150845633532 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1033 0.12576150845633532 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 950 0.11565675995500342 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.8261593677105804E-4 0.0 18-19 0.0 0.0 0.0 4.261038524658021E-4 0.0 20-21 0.0 0.0 0.0 6.695917681605462E-4 0.0 22-23 0.0 0.0 0.0 0.0018870313466342664 0.0 24-25 0.0 0.0 0.0 0.003469702798650103 0.0 26-27 0.0 0.0 0.0 0.00687853361837652 0.0 28-29 0.0 0.0 0.0 0.01728764201432683 0.0 30-31 0.0 0.0 0.0 0.04723665564478035 0.0 32-33 0.0 0.0 0.0 0.09696906242543182 0.0 34-35 0.0 0.0 0.0 0.1744590915952841 0.0 36-37 0.0 0.0 0.0 0.2821416223112847 0.0 38-39 0.0 0.0 0.0 0.42756477995997055 0.0 40-41 0.0 0.0 0.0 0.5774315920700855 0.0 42-43 0.0 0.0 0.0 0.7337508339461113 0.0 44-45 0.0 0.0 0.0 0.9146014833283824 0.0 46-47 0.0 0.0 0.0 1.138366877851852 0.0 48-49 0.0 0.0 0.0 1.3612191926914667 0.0 50-51 0.0 0.0 0.0 1.5940545120745657 0.0 52-53 0.0 0.0 0.0 1.8130110202630645 0.0 54-55 0.0 0.0 0.0 2.0592381750093742 0.0 56-57 0.0 0.0 0.0 2.3716940428246547 0.0 58-59 0.0 0.0 0.0 2.689263156869525 0.0 60-61 0.0 0.0 0.0 3.067399889943462 0.0 62-63 0.0 0.0 0.0 3.4296490365183177 0.0 64-65 0.0 0.0 0.0 3.800237644205718 0.0 66-67 0.0 0.0 0.0 4.237359324856707 0.0 68-69 0.0 0.0 0.0 4.7267091634242195 0.0 70-71 0.0 0.0 0.0 5.272730814369682 0.0 72-73 0.0 0.0 0.0 5.809134692645204 0.0 74-75 0.0 0.0 0.0 6.3810878066121575 0.0 76-77 0.0 0.0 0.0 6.99595566572031 0.0 78-79 0.0 0.0 0.0 7.686670010567376 0.0 80-81 0.0 0.0 0.0 8.419933868682097 0.0 82-83 0.0 0.0 0.0 9.174807279314727 0.0 84-85 0.0 0.0 0.0 9.974482466435191 0.0 86-87 0.0 0.0 0.0 10.805567594680276 0.0 88-89 0.0 0.0 0.0 11.64438346424867 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGCGAG 60 8.3051666E-5 39.56997 1 CGTACAG 55 0.002614579 34.449604 3 GTGTACG 70 2.0568931E-4 33.917118 1 TAGCCCG 105 1.9480121E-6 31.567259 9 GGACCGC 85 6.52445E-4 27.853466 6 ATATGAG 225 3.6379788E-12 27.368296 3 ATAGGAC 105 7.0352755E-5 27.067545 3 GATTGGC 175 5.125912E-9 27.05765 9 GTATAGT 160 6.257687E-8 26.709728 1 GTATAGG 185 8.993084E-9 25.667007 1 CTTTAGG 255 2.0008883E-11 24.148497 2 GAGTACT 5720 0.0 24.050158 12-13 GTACATG 5510 0.0 24.043604 1 TGTACGT 100 0.0016801957 23.684101 2 CCTTTAG 265 3.092282E-11 23.294022 1 TACATGG 5480 0.0 23.25191 2 GTACTTT 5885 0.0 23.175392 14-15 ACATGGG 5395 0.0 22.56465 3 ATTAGAC 105 0.002231429 22.556288 3 TTAGGCA 295 5.456968E-12 22.479826 4 >>END_MODULE