##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939082_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 821396 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.44047450925011 34.0 31.0 34.0 31.0 34.0 2 32.643156285153566 34.0 31.0 34.0 31.0 34.0 3 32.720330997472594 34.0 31.0 34.0 31.0 34.0 4 36.2025892504955 37.0 35.0 37.0 35.0 37.0 5 36.17686596964192 37.0 35.0 37.0 35.0 37.0 6 36.235687780315466 37.0 37.0 37.0 35.0 37.0 7 36.18973552342597 37.0 37.0 37.0 35.0 37.0 8 36.17797505709792 37.0 37.0 37.0 35.0 37.0 9 37.89135325713785 39.0 38.0 39.0 35.0 39.0 10-11 37.8223633911049 39.0 38.0 39.0 35.0 39.0 12-13 37.742537704103746 39.0 38.0 39.0 35.0 39.0 14-15 39.06707483357601 40.0 38.0 41.0 36.0 41.0 16-17 39.15726214882956 40.5 38.5 41.0 36.0 41.0 18-19 39.24806792338896 41.0 39.0 41.0 36.0 41.0 20-21 39.23445512274226 41.0 39.0 41.0 36.0 41.0 22-23 39.14985342027475 41.0 39.0 41.0 35.0 41.0 24-25 39.052908097921105 41.0 39.0 41.0 35.0 41.0 26-27 38.85967243570701 40.0 38.0 41.0 35.0 41.0 28-29 38.67668274498537 40.0 38.0 41.0 35.0 41.0 30-31 38.398534933211266 40.0 38.0 41.0 34.0 41.0 32-33 38.149002430009396 40.0 37.0 41.0 34.0 41.0 34-35 37.96444772070962 40.0 37.0 41.0 33.5 41.0 36-37 37.73785056172662 40.0 36.5 41.0 33.0 41.0 38-39 37.53419726903954 40.0 36.0 41.0 33.0 41.0 40-41 37.29546162873936 40.0 36.0 41.0 33.0 41.0 42-43 37.021342933250224 40.0 35.5 41.0 32.0 41.0 44-45 36.629656097667876 40.0 35.0 41.0 31.0 41.0 46-47 36.11904002454358 39.5 35.0 41.0 30.0 41.0 48-49 35.60658379149643 39.0 35.0 41.0 27.5 41.0 50-51 35.2630759097926 39.0 35.0 41.0 27.0 41.0 52-53 34.95773232399476 39.0 35.0 40.0 26.0 41.0 54-55 34.68284298925245 38.0 35.0 40.0 24.5 41.0 56-57 34.43101987348368 38.0 34.5 40.0 23.5 41.0 58-59 34.09761674028118 38.0 34.0 40.0 22.5 41.0 60-61 33.6932630546046 37.0 34.0 40.0 21.5 41.0 62-63 33.28635213709343 36.5 33.0 40.0 20.0 41.0 64-65 32.81606679360503 36.0 33.0 39.0 17.5 41.0 66-67 32.36812572742015 35.5 33.0 39.0 12.0 40.0 68-69 31.857342256353817 35.0 32.0 38.0 7.5 40.0 70-71 31.335028415039762 35.0 31.5 37.5 3.5 39.5 72-73 30.76677570867158 35.0 31.0 37.0 2.0 39.0 74-75 30.251964947479657 34.5 30.5 36.0 2.0 39.0 76-77 28.285833507833004 32.5 27.5 34.5 2.0 36.5 78-79 29.22709083073207 34.0 29.0 35.0 2.0 37.0 80-81 29.23895782302324 34.0 29.5 35.0 2.0 37.0 82-83 28.870803485773003 34.0 29.0 35.0 2.0 36.0 84-85 28.422926335165986 34.0 29.0 35.0 2.0 36.0 86-87 27.773955558585627 33.5 27.5 35.0 2.0 35.5 88-89 27.091611110840567 33.0 26.0 35.0 2.0 35.0 90-91 26.53275399441926 33.0 25.0 35.0 2.0 35.0 92-93 26.12040051327253 32.5 24.0 34.5 2.0 35.0 94-95 25.628622491465748 32.0 23.5 34.0 2.0 35.0 96-97 24.859433208829845 32.0 18.5 34.0 2.0 35.0 98-99 23.710232944888944 31.5 2.0 34.0 2.0 35.0 100-101 18.10314330724766 23.5 2.0 30.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13935107629131238 1101 2 0.18570088061688494 1101 3 0.2569841180618937 1101 4 0.05487310621147401 1101 5 0.09333859970337244 1101 6 0.14167334197208703 1101 7 0.17425152203102812 1101 8 0.08531594909356954 1101 9 0.12395783179520947 1101 10-11 0.17886222663069162 1101 12-13 0.17209343835532565 1101 14-15 0.27436294188361643 1101 16-17 0.24145319280460598 1101 18-19 0.1783685666462489 1101 20-21 0.2963408521093669 1101 22-23 0.2645603615301013 1101 24-25 0.3886232692071516 1101 26-27 0.43123885751713686 1101 28-29 0.4727412455933262 1101 30-31 0.48151546941006984 1101 32-33 0.5292564607917214 1101 34-35 0.5508923697444814 1101 36-37 0.3450169396327425 1101 38-39 0.4566564238828903 1101 40-41 0.6541755978836648 1101 42-43 0.6968379846668782 1101 44-45 0.6192362525372133 1101 46-47 0.4989581065780442 1101 48-49 0.5329558256619364 1101 50-51 0.6068260208315834 1101 52-53 0.7508581961109186 1101 54-55 0.5755284179741551 1101 56-57 0.6008283270658481 1101 58-59 0.5884584754835132 1101 60-61 0.4047046178543141 1101 62-63 0.3993452845237968 1101 64-65 0.350132024587424 1101 66-67 0.3458696797848759 1101 68-69 0.5208386275838137 1101 70-71 0.5020813832833824 1101 72-73 0.6836091080606259 1101 74-75 0.3930031386830102 1101 76-77 0.5635120568006577 1101 78-79 0.5980048373665703 1101 80-81 0.49983603145113165 1101 82-83 0.4482647488597884 1101 84-85 0.5186558957147653 1101 86-87 0.5819188611861357 1101 88-89 0.5096451050702591 1101 90-91 0.5736540679145925 1101 92-93 0.5585474966127286 1101 94-95 0.4118443662951208 1101 96-97 0.38808778444709446 1101 98-99 0.7863135099054439 1101 100-101 -0.624802738771816 1102 1 0.07064534432027614 1102 2 -0.00971756990360717 1102 3 0.03549732514652959 1102 4 0.08622078041629067 1102 5 0.10945238112327615 1102 6 0.20591834499259676 1102 7 0.11794302694702452 1102 8 0.18045336055751449 1102 9 0.2295400904386966 1102 10-11 0.28453203638213864 1102 12-13 0.07856040094473116 1102 14-15 0.1788271728784636 1102 16-17 0.21402780823637357 1102 18-19 0.18635103891443094 1102 20-21 0.1935624176982813 1102 22-23 0.2537456109194238 1102 24-25 0.35323073381461967 1102 26-27 0.32130968636347745 1102 28-29 0.37772859445823315 1102 30-31 0.1504759751052731 1102 32-33 0.17024876504932962 1102 34-35 0.2616344529355459 1102 36-37 0.2752321402561009 1102 38-39 0.5389369551430079 1102 40-41 0.4210707729421088 1102 42-43 0.4497972121158966 1102 44-45 0.3969177240962338 1102 46-47 0.2616573947875409 1102 48-49 0.28797250414800857 1102 50-51 0.27378160615451463 1102 52-53 0.3735763953597271 1102 54-55 0.40795340856241324 1102 56-57 0.30566508802505865 1102 58-59 0.34259088777919544 1102 60-61 0.1498893069165561 1102 62-63 0.3003140375333615 1102 64-65 0.4130550058777587 1102 66-67 0.5227207290849023 1102 68-69 0.44893071383916805 1102 70-71 0.20101282201074255 1102 72-73 0.31553330631135523 1102 74-75 0.05760359920184044 1102 76-77 0.24272041968248814 1102 78-79 0.2937734923188984 1102 80-81 0.5452794340373934 1102 82-83 0.2596533969831327 1102 84-85 0.2215424557441814 1102 86-87 0.47870704879064974 1102 88-89 0.5018267911294991 1102 90-91 0.5155945506714019 1102 92-93 0.4438599227414848 1102 94-95 0.7053310016184895 1102 96-97 0.45035850998312554 1102 98-99 0.6447871438892783 1102 100-101 0.4785119461755194 1103 1 0.2504749661386114 1103 2 0.1229189497498453 1103 3 0.18622825581666547 1103 4 0.0722969129969826 1103 5 0.16058030949827184 1103 6 0.15804290302249768 1103 7 0.19712545979858476 1103 8 0.092146183583381 1103 9 0.20323072170639733 1103 10-11 0.08667003443850518 1103 12-13 0.21692443956930418 1103 14-15 0.35635131323368796 1103 16-17 0.31991143828413016 1103 18-19 0.2051815498422087 1103 20-21 0.25384197024877864 1103 22-23 0.20017469927209675 1103 24-25 0.27714157474672874 1103 26-27 0.30286686850685385 1103 28-29 0.15902007761684445 1103 30-31 0.1158679069540014 1103 32-33 0.0727393053383949 1103 34-35 0.12916851823339925 1103 36-37 0.173206831652422 1103 38-39 0.2799424758923479 1103 40-41 0.31754535061512 1103 42-43 0.31216927340241796 1103 44-45 0.4848454787847416 1103 46-47 0.4770968836529974 1103 48-49 0.4408435005708924 1103 50-51 0.6557430229400723 1103 52-53 0.848212997721042 1103 54-55 0.8917126873499157 1103 56-57 0.8605944761936968 1103 58-59 0.7543136924025617 1103 60-61 0.7395394526891508 1103 62-63 0.8552330872201068 1103 64-65 0.9388867367463902 1103 66-67 0.7811869119106234 1103 68-69 0.7460638528090442 1103 70-71 0.7096495897877553 1103 72-73 0.46162329245779077 1103 74-75 0.3597209202944107 1103 76-77 0.35681280435672136 1103 78-79 0.39301154620306633 1103 80-81 0.35960856994707413 1103 82-83 0.4037180745258837 1103 84-85 0.1517783799010779 1103 86-87 0.4121437669429575 1103 88-89 0.5563705539233688 1103 90-91 0.5978154633525854 1103 92-93 0.22299066318355543 1103 94-95 0.19386148980160556 1103 96-97 0.06865452139680883 1103 98-99 -0.16130325579642957 1103 100-101 -0.6293359954327329 1104 1 0.05460239318197324 1104 2 0.11153655235419535 1104 3 0.2408614380047851 1104 4 0.19949723854052337 1104 5 0.1266423953022482 1104 6 0.14281792918225023 1104 7 0.22729637015784476 1104 8 0.19060566503902265 1104 9 0.20608504097160107 1104 10-11 0.3739651164057136 1104 12-13 0.21978457211202596 1104 14-15 0.13177568249307825 1104 16-17 0.12658217424506546 1104 18-19 0.1882329392155384 1104 20-21 0.28155967749212607 1104 22-23 0.29812261230546255 1104 24-25 0.1780746057896394 1104 26-27 0.2971436967826335 1104 28-29 0.406293650457215 1104 30-31 0.4593744759576026 1104 32-33 0.41775151322119797 1104 34-35 0.33289773576624526 1104 36-37 0.4682015997026454 1104 38-39 0.44800432916522226 1104 40-41 0.5029423974701359 1104 42-43 0.4852247320700229 1104 44-45 0.1770440603450183 1104 46-47 0.5346803041854926 1104 48-49 0.5701918321602406 1104 50-51 0.4484299198125328 1104 52-53 0.46938204783149473 1104 54-55 0.3934159776649935 1104 56-57 0.3582866050159268 1104 58-59 0.47364284017606906 1104 60-61 0.5762880865689297 1104 62-63 0.678119960839453 1104 64-65 0.3659039440478651 1104 66-67 0.48098635383598065 1104 68-69 0.48103653040466554 1104 70-71 0.39004721797052966 1104 72-73 0.33593151243221087 1104 74-75 0.41967325141869694 1104 76-77 0.3427359528278302 1104 78-79 0.4164216147997415 1104 80-81 0.851304303001374 1104 82-83 0.6943325379603671 1104 84-85 0.34733703587131615 1104 86-87 0.08205424246905935 1104 88-89 0.14202338498952471 1104 90-91 0.4512016975114044 1104 92-93 0.2891079751240291 1104 94-95 0.47269825296868007 1104 96-97 0.15580427142587538 1104 98-99 -0.3310189865254145 1104 100-101 -0.3580216133841354 1105 1 -0.011729923846289125 1105 2 0.08352188221147117 1105 3 0.010660975512337245 1105 4 0.059074072676587264 1105 5 -0.06784009921117473 1105 6 0.02020956013094377 1105 7 0.04623941288684108 1105 8 0.030335092225925564 1105 9 0.050954909735686726 1105 10-11 0.06371405959573195 1105 12-13 0.03271079425569923 1105 14-15 -0.11396946059972635 1105 16-17 0.01841748863682824 1105 18-19 0.08228865924558448 1105 20-21 0.034450255313110745 1105 22-23 -0.0904152340115374 1105 24-25 -0.04906037130667329 1105 26-27 -0.11709451628792777 1105 28-29 0.021960950473413732 1105 30-31 -0.06127934696964843 1105 32-33 0.14750273941535852 1105 34-35 0.09733773694456715 1105 36-37 0.20483497869606282 1105 38-39 0.13103498033691352 1105 40-41 0.04142942853371778 1105 42-43 0.09254433792040118 1105 44-45 0.159074608413313 1105 46-47 0.22942239836309852 1105 48-49 0.33750768210058624 1105 50-51 0.23326782651867006 1105 52-53 0.2930439128249418 1105 54-55 0.3126786409734379 1105 56-57 0.3716753196109579 1105 58-59 0.4993376835325307 1105 60-61 0.3884612855732499 1105 62-63 0.42743874186253095 1105 64-65 0.3065034903928492 1105 66-67 0.2714967893742539 1105 68-69 0.01807643142539206 1105 70-71 0.05528148365329244 1105 72-73 -0.10345288654853846 1105 74-75 0.024378449303604555 1105 76-77 -0.1947623901530342 1105 78-79 -0.1483162815960597 1105 80-81 0.0737738506719694 1105 82-83 0.15234551426131304 1105 84-85 0.0869640960984377 1105 86-87 0.21527121623660506 1105 88-89 0.3650618425624721 1105 90-91 0.5493285294381103 1105 92-93 0.39673136781546603 1105 94-95 0.3033011869972242 1105 96-97 0.0684480638639755 1105 98-99 0.12063840083410682 1105 100-101 -0.5732923334878279 1106 1 -0.02557794184870943 1106 2 -0.01708644143649707 1106 3 0.009659467067514527 1106 4 0.0762975450671064 1106 5 0.10272544395260041 1106 6 0.12797681451409204 1106 7 0.16115323370338075 1106 8 0.1405999355702221 1106 9 0.1413008628997119 1106 10-11 -6.606776628572675E-4 1106 12-13 0.069840792524694 1106 14-15 0.04296443984291187 1106 16-17 -0.011819411385424416 1106 18-19 -0.08344862407002296 1106 20-21 0.06976270121928962 1106 22-23 0.177723196711284 1106 24-25 0.11273269973860778 1106 26-27 -0.02498046796824127 1106 28-29 0.11187415307486503 1106 30-31 0.07358427478163065 1106 32-33 0.14491241819079903 1106 34-35 0.06397885072087206 1106 36-37 0.10975349794728118 1106 38-39 0.1857593800500652 1106 40-41 0.09642584288619815 1106 42-43 0.18031851631254625 1106 44-45 0.21461505204445785 1106 46-47 0.2899827485201172 1106 48-49 0.3327474259122525 1106 50-51 0.4664956511434184 1106 52-53 0.16661274489299416 1106 54-55 0.1458903920058532 1106 56-57 0.14917352678719453 1106 58-59 0.10215303504963202 1106 60-61 -0.0619022248993204 1106 62-63 -0.06377642171717923 1106 64-65 0.07496957511305169 1106 66-67 0.050818731064204314 1106 68-69 -0.08770635252079728 1106 70-71 -0.09485205080601489 1106 72-73 -0.07523754986859998 1106 74-75 -0.20438586237571243 1106 76-77 -0.023127334884609496 1106 78-79 0.3426206851934275 1106 80-81 0.38285666676534547 1106 82-83 0.41834492077482466 1106 84-85 0.3599060580658424 1106 86-87 -0.07308903006028444 1106 88-89 0.02234194345517082 1106 90-91 0.00606583748232481 1106 92-93 -0.0810685283243977 1106 94-95 0.24164041398107727 1106 96-97 0.0455988341599749 1106 98-99 0.007647228945582896 1106 100-101 -0.5972296217491575 1107 1 0.18077496523743974 1107 2 0.1676637837996111 1107 3 0.09988546922245689 1107 4 0.1295265398229759 1107 5 0.09083164548103895 1107 6 0.17983857200516695 1107 7 0.1651700135848415 1107 8 0.09840209750373674 1107 9 0.20724632995029424 1107 10-11 0.2448233593008453 1107 12-13 0.18808433395231106 1107 14-15 0.26851528991144136 1107 16-17 0.1565439506526758 1107 18-19 0.29641768310705885 1107 20-21 0.15074194952190112 1107 22-23 0.24618747784590767 1107 24-25 0.2035665479369655 1107 26-27 0.1888131408220417 1107 28-29 0.22273361407363268 1107 30-31 0.13848724423445447 1107 32-33 -0.02460585132980242 1107 34-35 -0.03949153385416082 1107 36-37 0.12728777463546948 1107 38-39 -0.09716963852005023 1107 40-41 -0.07511913522301228 1107 42-43 -0.02307093314546904 1107 44-45 -0.1410414966223641 1107 46-47 -0.5428387728935462 1107 48-49 -0.7116811238670024 1107 50-51 -0.5319722437608334 1107 52-53 -0.5339672901122796 1107 54-55 -0.49443544678005225 1107 56-57 -0.5795922459849692 1107 58-59 -0.5922902529145162 1107 60-61 -0.5323792258368982 1107 62-63 -0.42515838051354393 1107 64-65 -0.6578104795431372 1107 66-67 -0.5109792275722853 1107 68-69 -0.3322927757381855 1107 70-71 -0.16704167878758014 1107 72-73 -0.3418038525492868 1107 74-75 -0.35630907060378547 1107 76-77 -0.360606788855673 1107 78-79 0.01401328545887992 1107 80-81 -0.017630609268369568 1107 82-83 -0.06786446803181434 1107 84-85 -0.2664901143359444 1107 86-87 -0.05688035734624464 1107 88-89 -0.18838329181455293 1107 90-91 -0.055525620032565115 1107 92-93 -0.05369880305415364 1107 94-95 0.035519820656723766 1107 96-97 0.3111401252249735 1107 98-99 0.34650807550026386 1107 100-101 -0.6175766435533454 1108 1 0.10721255479109004 1108 2 0.10756642576152586 1108 3 0.12106346649668609 1108 4 0.1593223614742456 1108 5 0.08014643382033881 1108 6 0.08695404194615008 1108 7 0.06696275641935756 1108 8 0.1352000008549723 1108 9 0.23685608674603031 1108 10-11 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5 26.0 6 78.0 7 172.0 8 331.0 9 611.0 10 1085.0 11 1806.0 12 2666.0 13 3657.0 14 4552.0 15 6177.0 16 8439.0 17 12704.0 18 12184.0 19 7398.0 20 6301.0 21 6289.0 22 6569.0 23 6898.0 24 7574.0 25 8739.0 26 9565.0 27 10922.0 28 13023.0 29 15801.0 30 18954.0 31 22874.0 32 28723.0 33 37332.0 34 53535.0 35 84139.0 36 126995.0 37 183616.0 38 107367.0 39 4281.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.33770595218156 20.497109303653318 15.562814727697505 20.602370016467617 2 15.76109452687863 22.623314454903603 38.58784313534519 23.027747882872575 3 19.328557723680174 26.81142834881105 31.629080248747254 22.230933678761524 4 12.699720962848616 18.422660933337877 37.35348114673069 31.52413695708282 5 12.441136796380796 38.87150655712957 33.662691320629754 15.024665325859878 6 28.468728846987325 37.83777861104753 17.217517494606742 16.4759750473584 7 25.79961431514154 35.95756492605272 20.97064022712553 17.272180531680213 8 25.489167222630744 33.2552142937146 20.238715552547127 21.016902931107527 9 26.20636087830961 17.879439393422906 21.874954346015805 34.03924538225168 10-11 24.365166131804877 27.18524317138141 26.606350651817152 21.843240044996566 12-13 26.76242640577748 25.64420815294937 27.215131313032938 20.378234128240216 14-15 23.30887070559747 26.793699452999736 27.584859305396105 22.31257053600669 16-17 20.062687528420316 30.00138182840856 28.41587069495012 21.520059948221 18-19 19.843778153290252 27.98187475955568 32.182345665184634 19.992001421969427 20-21 21.313592956381573 26.511633852611894 31.70255272730814 20.472220463698388 22-23 20.688194244919625 26.63270821869111 31.84115822331738 20.837939313071892 24-25 21.063361072711015 27.038132056968898 32.02148051699821 19.877026353321877 26-27 20.75584371787013 26.499576623789302 32.88771795048173 19.85686170785885 28-29 19.794471850362065 27.526613229185436 32.84779813877837 19.831116781674126 30-31 20.06845662749758 27.330727201009015 32.117517007630916 20.483299163862497 32-33 20.14576404073066 27.563258160497494 31.79824347817618 20.49273432059567 34-35 21.12370973977152 26.91052876698151 31.49333828484806 20.472423208398904 36-37 21.189063002328368 26.515022358179003 31.92098892320282 20.37492571628981 38-39 20.677150876423923 26.873793737480746 31.97458705486249 20.47446833123284 40-41 20.233610609211468 27.166197505491134 32.277700395946056 20.32249148935134 42-43 20.329288003725722 27.642111607265157 30.911918959476694 21.116681429532427 44-45 20.35478875331462 27.35164275922188 30.91225306259603 21.381315424867473 46-47 21.191574846289647 27.828757533329274 29.967918670481524 21.011748949899555 48-49 21.07092897119572 27.56507108237325 29.923871186102264 21.440128760328765 50-51 20.597899663011084 28.152209907084558 30.117410444708494 21.13247998519586 52-53 20.98451903832013 28.428735956834462 29.513718109170238 21.073026895675167 54-55 20.228915163940414 27.79256290510302 29.76518025410399 22.213341676852576 56-57 20.91898538462007 28.397786692151765 28.877458470041816 21.805769453186347 58-59 21.19677567806453 27.39857946728806 29.305000596555566 22.09964425809185 60-61 20.69662625871559 27.904861737602772 29.96683601725404 21.431675986427603 62-63 20.661844735605428 27.983250262361427 29.88795767154541 21.466947330487738 64-65 20.283622147997047 28.253129358167318 29.688649939826472 21.774598554009163 66-67 20.39062428664043 27.91694440132582 29.1490210043494 22.543410307684347 68-69 20.120982420598057 28.03207854300008 29.49404584353492 22.352893192866944 70-71 20.66838772356036 28.195343406852615 28.907912093397265 22.228356776189763 72-73 20.70542101495503 27.568858382558474 29.17332200302899 22.55239859945751 74-75 20.08318764639711 28.96294844386873 28.414796273660937 22.53906763607322 76-77 20.535690893193888 27.781714491133258 29.081093925647906 22.60150069002495 78-79 20.274891494205008 27.98752419272845 29.24904675018371 22.48853756288283 80-81 20.532923947489206 28.5441926093843 28.5574018770552 22.365481566071296 82-83 20.3165099416116 28.164734184242434 28.773027869626834 22.745728004519137 84-85 20.108266901713666 27.854104475794866 28.631561390608184 23.40606723188328 86-87 20.442276133432376 27.863128278847615 28.50407462229077 23.190520965429236 88-89 20.474792140140515 27.42197538078606 28.340397491867154 23.762834987206276 90-91 20.045932941817256 27.415315341199108 28.876134492925143 23.662617224058494 92-93 20.291491558273965 27.65827931959737 28.795063525997204 23.255165596131462 94-95 19.884014531358808 27.134902044811515 28.822334172555014 24.158749251274656 96-97 19.642474518989623 27.05984689479861 28.77381920535284 24.52385938085893 98-99 19.8745668948154 27.373160748678465 27.995273888048857 24.75699846845728 100-101 20.135890936627245 26.042313433017526 29.12889551177632 24.692900118578905 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2571.0 1 1670.5 2 908.0 3 1009.0 4 838.5 5 878.5 6 1497.5 7 2828.5 8 4182.5 9 4491.0 10 3628.5 11 2738.5 12 2573.5 13 2539.5 14 2496.0 15 2472.0 16 2383.0 17 2370.5 18 2419.0 19 2531.0 20 2717.5 21 2963.0 22 3310.5 23 3762.0 24 4157.5 25 4767.5 26 6091.0 27 7653.0 28 8360.0 29 8962.0 30 10438.0 31 12154.0 32 14923.0 33 17227.5 34 18458.0 35 19437.5 36 20866.5 37 22943.5 38 23020.5 39 22033.0 40 21984.0 41 23068.0 42 23627.5 43 23824.5 44 24133.5 45 24826.0 46 25378.0 47 28926.5 48 42013.5 49 41837.5 50 29075.0 51 23223.0 52 20240.5 53 20149.0 54 20547.0 55 19859.0 56 18094.0 57 16210.5 58 15294.0 59 13940.5 60 11816.0 61 10255.0 62 9097.0 63 7349.5 64 5545.5 65 4373.0 66 3381.0 67 3079.0 68 2494.0 69 1586.0 70 1270.0 71 1111.0 72 966.5 73 810.0 74 649.5 75 545.5 76 370.0 77 192.5 78 117.0 79 73.5 80 34.0 81 19.0 82 10.5 83 4.5 84 3.5 85 1.0 86 0.5 87 1.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4172165435429439 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.522077049316042E-4 16-17 0.0024957511358711265 18-19 0.0 20-21 0.0 22-23 0.0 24-25 6.0871978923686016E-5 26-27 0.0027392390515658706 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011991779847966146 36-37 0.026783670726421845 38-39 0.019600777213426897 40-41 0.008765564965010787 42-43 0.009556900691018704 44-45 0.004139294566810649 46-47 0.0056002220609791134 48-49 0.003043598946184301 50-51 4.8697583138948813E-4 52-53 0.0 54-55 0.0 56-57 1.2174395784737203E-4 58-59 0.0017044154098632084 60-61 0.0028001110304895567 62-63 0.0012174395784737204 64-65 0.0026174950937184987 66-67 0.0022522632201763823 68-69 0.004504526440352765 70-71 1.8261593677105804E-4 72-73 0.0 74-75 0.0 76-77 0.09416895139494227 78-79 0.015400610667692563 80-81 3.6523187354211607E-4 82-83 0.0 84-85 0.0 86-87 0.1084129944630848 88-89 0.41599910396447026 90-91 0.42178194196222046 92-93 0.0 94-95 0.0 96-97 0.0 98-99 2.4348791569474406E-4 100-101 2.4348791569474406E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 821396.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.920865762691776 #Duplication Level Percentage of deduplicated Percentage of total 1 66.69891705489195 22.62485011935903 2 14.557665002453883 9.876172007329483 3 6.392882321568062 6.5055630919978675 4 3.3339187036576434 4.523576352419932 5 2.0569695285830343 3.4887093628506243 6 1.3473644863555272 2.7422261925050395 7 0.9242612050130821 2.194621818543875 8 0.7201857615743836 1.9543459634053288 9 0.5094904502729161 1.555412145397288 >10 2.995873698920469 19.23173696693603 >50 0.29743144214227013 6.972575815848798 >100 0.14817752675240656 9.165287635335243 >500 0.008252017228341785 1.8932028636055513 >1k 0.007893233870587794 5.304521728793492 >5k 7.175667155079813E-4 1.9671979356724176 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8869 1.0797471621483425 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7295 0.8881221724965791 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4997 0.6083545573633181 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4933 0.6005629440610862 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3541 0.4310953547375444 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3069 0.37363220663358476 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2728 0.33211751700763087 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2525 0.3074034935646144 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1941 0.23630502218174912 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1771 0.21560854934769588 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1671 0.20343415356295866 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1605 0.19539905234503213 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1559 0.189798830284053 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1446 0.17604176304729996 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1324 0.16118900018992058 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1320 0.1607020243585311 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1261 0.15351913084553612 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1256 0.15291041105629927 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1209 0.14718844503747278 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1140 0.1387881119460041 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1128 0.13732718445183567 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1098 0.1336748657164145 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1045 0.12722243595050378 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1019 0.1240570930464721 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 981 0.11943082264827196 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 940 0.11443932037652972 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 857 0.10433457187519783 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 834 0.10153446084470827 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.8261593677105804E-4 0.0 18-19 0.0 0.0 0.0 4.261038524658021E-4 0.0 20-21 0.0 0.0 0.0 6.695917681605462E-4 0.0 22-23 0.0 0.0 0.0 0.0018870313466342664 0.0 24-25 0.0 0.0 0.0 0.003469702798650103 0.0 26-27 0.0 0.0 0.0 0.00687853361837652 0.0 28-29 0.0 0.0 0.0 0.017226770035403145 0.0 30-31 0.0 0.0 0.0 0.047784503455093524 0.0 32-33 0.0 0.0 0.0 0.09788214210928711 0.0 34-35 0.0 0.0 0.0 0.17610263502622364 0.0 36-37 0.0 0.0 0.0 0.28469824542607947 0.0 38-39 0.0 0.0 0.0 0.4317040745267812 0.0 40-41 0.0 0.0 0.0 0.5824839663207515 0.0 42-43 0.0 0.0 0.0 0.7397771598595562 0.0 44-45 0.0 0.0 0.0 0.9242801279772486 0.0 46-47 0.0 0.0 0.0 1.1509065055101315 0.0 48-49 0.0 0.0 0.0 1.3782633467900989 0.0 50-51 0.0 0.0 0.0 1.6131683134566033 0.0 52-53 0.0 0.0 0.0 1.8329770293500334 0.0 54-55 0.0 0.0 0.0 2.0821869110636038 0.0 56-57 0.0 0.0 0.0 2.3977472498039925 0.0 58-59 0.0 0.0 0.0 2.716716419364107 0.0 60-61 0.0 0.0 0.0 3.100209886583329 0.0 62-63 0.0 0.0 0.0 3.467207047514232 0.0 64-65 0.0 0.0 0.0 3.8395000706114955 0.0 66-67 0.0 0.0 0.0 4.282282845302388 0.0 68-69 0.0 0.0 0.0 4.773884947090076 0.0 70-71 0.0 0.0 0.0 5.325628564054366 0.0 72-73 0.0 0.0 0.0 5.8648934253393 0.0 74-75 0.0 0.0 0.0 6.4400727541892095 0.0 76-77 0.0 0.0 0.0 7.05457538142382 0.0 78-79 0.0 0.0 0.0 7.7465071658493585 0.0 80-81 0.0 0.0 0.0 8.482510263015646 0.0 82-83 0.0 0.0 0.0 9.239148961037063 0.0 84-85 0.0 0.0 0.0 10.036937116810893 0.0 86-87 0.0 0.0 0.0 10.86887445276091 0.0 88-89 0.0 0.0 0.0 11.70501195525666 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCGC 25 0.0050396323 56.946014 6 GTAAGCG 95 0.0 32.46922 94-95 CGTATAG 80 4.4075193E-4 29.78449 1 AATTCGC 120 0.0 27.684616 78-79 CAATTCG 130 0.0 25.55503 78-79 GTGTAGA 235 1.6916601E-10 24.334562 1 CGCGGAA 130 2.3646862E-11 23.845812 82-83 GTATAGG 280 1.8189894E-12 23.827593 1 TCGCGCT 140 1.8189894E-12 23.72895 60-61 AGGACGG 100 0.0016623881 23.727505 5 TAAGGTG 330 0.0 23.00849 5 ACCGTCC 125 2.2696568E-4 22.778404 8 GAGTACT 6320 0.0 22.530228 12-13 TATAGGG 130 2.9594198E-4 21.902313 2 GTACTTT 6595 0.0 21.518787 14-15 AGTACTT 6420 0.0 21.070326 12-13 ATTCGCG 160 1.8189894E-11 20.77738 80-81 CTCGCGC 160 1.8189894E-11 20.762833 60-61 TAGGACA 415 0.0 20.582897 4 AGGTAAG 255 1.2036253E-8 20.470789 2 >>END_MODULE