##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939081_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 502100 Sequences flagged as poor quality 0 Sequence length 101 %GC 27 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.51323242381996 33.0 31.0 34.0 31.0 34.0 2 32.50291774546903 34.0 31.0 34.0 31.0 34.0 3 32.473535152360085 34.0 31.0 34.0 31.0 34.0 4 35.98135232025493 37.0 35.0 37.0 35.0 37.0 5 36.06864568810994 37.0 37.0 37.0 35.0 37.0 6 36.25309101772555 37.0 37.0 37.0 35.0 37.0 7 36.233230432184826 37.0 37.0 37.0 35.0 37.0 8 36.24116510655248 37.0 37.0 37.0 35.0 37.0 9 37.90991635132444 39.0 39.0 39.0 37.0 39.0 10-11 37.90764887472615 39.0 38.0 39.0 37.0 39.0 12-13 37.79937761402111 39.0 38.0 39.0 35.0 39.0 14-15 38.943499302927705 40.5 38.5 41.0 36.0 41.0 16-17 38.97543118900617 40.0 38.5 41.0 36.5 41.0 18-19 39.034664409480186 40.5 38.5 41.0 35.5 41.0 20-21 39.01438657637921 41.0 39.0 41.0 35.0 41.0 22-23 38.822355108544116 41.0 38.0 41.0 35.0 41.0 24-25 38.7043367855009 41.0 38.0 41.0 35.0 41.0 26-27 38.4767038438558 40.0 37.0 41.0 35.0 41.0 28-29 38.209315873332 40.0 36.0 41.0 35.0 41.0 30-31 37.64982473610834 39.0 35.0 41.0 35.0 41.0 32-33 37.016595299741084 38.0 35.0 41.0 35.0 41.0 34-35 36.59233120892252 37.5 35.0 41.0 34.5 41.0 36-37 36.27707926707827 36.5 35.0 40.5 33.5 41.0 38-39 35.980392352121086 35.0 35.0 40.0 33.0 41.0 40-41 35.74815475004979 35.0 35.0 40.0 33.0 41.0 42-43 35.44702748456483 35.0 35.0 40.0 33.0 41.0 44-45 35.05005576578371 35.0 35.0 40.0 32.0 41.0 46-47 34.49494224258116 35.0 35.0 40.0 30.0 41.0 48-49 34.07874626568413 35.0 35.0 40.0 24.0 41.0 50-51 33.06234714200359 35.0 34.0 39.0 23.0 40.0 52-53 33.35086138219478 35.0 34.0 39.5 23.0 40.5 54-55 33.73973411670982 35.0 35.0 40.0 23.5 41.0 56-57 33.61240689105756 35.0 35.0 40.0 23.0 41.0 58-59 33.44390260904203 35.0 35.0 40.0 22.0 41.0 60-61 33.166553475403305 35.0 35.0 39.5 19.5 41.0 62-63 32.83718781119299 35.0 35.0 39.0 10.5 41.0 64-65 32.506374228241384 35.0 35.0 39.0 2.0 41.0 66-67 32.146350328619796 35.0 34.5 38.0 2.0 40.5 68-69 31.789142601075483 35.0 34.0 37.0 2.0 40.0 70-71 31.389178450507867 35.0 33.5 37.0 2.0 39.5 72-73 30.95964748058156 35.0 33.0 36.0 2.0 39.0 74-75 30.55380999800836 35.0 33.0 35.5 2.0 39.0 76-77 30.131370244971123 35.0 33.0 35.0 2.0 37.5 78-79 29.72958076080462 35.0 32.5 35.0 2.0 37.0 80-81 29.31894343756224 35.0 31.5 35.0 2.0 37.0 82-83 28.879017128062138 35.0 31.0 35.0 2.0 36.0 84-85 28.485175263891655 35.0 31.0 35.0 2.0 36.0 86-87 28.093807010555665 35.0 29.5 35.0 2.0 35.0 88-89 27.66815972913762 35.0 29.0 35.0 2.0 35.0 90-91 27.301232822146986 35.0 28.0 35.0 2.0 35.0 92-93 26.873784106751643 35.0 26.5 35.0 2.0 35.0 94-95 26.405819557857 34.0 24.5 35.0 2.0 35.0 96-97 25.866212905795656 34.0 18.5 35.0 2.0 35.0 98-99 25.318660625373433 34.0 2.0 35.0 2.0 35.0 100-101 23.51471021708823 31.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10624051711478444 1101 2 0.15861091285036366 1101 3 0.08754496555012992 1101 4 0.2267024088339653 1101 5 0.024555293251715682 1101 6 0.2488724433821332 1101 7 0.07723668006356377 1101 8 0.16232987604870885 1101 9 0.4047458023392423 1101 10-11 0.3328579094706541 1101 12-13 0.4055235275123792 1101 14-15 0.3240910676048543 1101 16-17 0.27219421868900184 1101 18-19 0.28464161713028346 1101 20-21 0.350964102444955 1101 22-23 0.4904231920694855 1101 24-25 0.4213637313748464 1101 26-27 0.2350693668809214 1101 28-29 0.3096321079705362 1101 30-31 0.28549741605079504 1101 32-33 0.21910737237629263 1101 34-35 0.25591978966939166 1101 36-37 0.2029722970121739 1101 38-39 0.4214211901402365 1101 40-41 0.4145020489465381 1101 42-43 0.5924515268421899 1101 44-45 0.540394490953112 1101 46-47 0.4152977805408895 1101 48-49 0.4742130896774128 1101 50-51 0.21938150951909563 1101 52-53 0.23318098780206498 1101 54-55 0.44231743564349557 1101 56-57 0.3442422778625627 1101 58-59 0.3831605897325403 1101 60-61 0.345796796711376 1101 62-63 0.4814750464929958 1101 64-65 0.48979969886910624 1101 66-67 0.33531961184313985 1101 68-69 0.39589287881291924 1101 70-71 0.32900137951052244 1101 72-73 0.28859006107682816 1101 74-75 0.49566434428198036 1101 76-77 0.6518459663141591 1101 78-79 0.9355054037546786 1101 80-81 1.095193256099126 1101 82-83 1.441032012368062 1101 84-85 1.4119022213116175 1101 86-87 1.3683154406845652 1101 88-89 1.493384389995093 1101 90-91 1.520651862473617 1101 92-93 1.5508228097758696 1101 94-95 1.7758019546588564 1101 96-97 1.601989649894012 1101 98-99 1.5131586913422943 1101 100-101 1.701477880043683 1102 1 0.027233745105753826 1102 2 0.05477344106706994 1102 3 0.22072780979392093 1102 4 0.1905850273441203 1102 5 0.16225281018174087 1102 6 0.0976309083934197 1102 7 0.13592742498455834 1102 8 0.15781520336248178 1102 9 0.17901216802773234 1102 10-11 0.21547641962867914 1102 12-13 0.07595242141756131 1102 14-15 0.22363960033622732 1102 16-17 0.317340945551301 1102 18-19 0.3534903755952925 1102 20-21 0.26969999409281087 1102 22-23 0.3301523117083107 1102 24-25 0.2588355146705581 1102 26-27 0.2768300892285467 1102 28-29 0.21595264973126405 1102 30-31 -0.004570304039496875 1102 32-33 0.07576651458848005 1102 34-35 0.1735270131456872 1102 36-37 0.3248684595403901 1102 38-39 -0.10002350511935987 1102 40-41 -0.4342564160647484 1102 42-43 -0.32854170114877945 1102 44-45 -0.31062131040128094 1102 46-47 -0.22804307724691597 1102 48-49 -0.25829255366344483 1102 50-51 -0.4194446755824828 1102 52-53 -0.4835233011369837 1102 54-55 -0.5125358374941982 1102 56-57 -0.3453739749591094 1102 58-59 -0.6529567917573047 1102 60-61 -0.5311783725888475 1102 62-63 -0.6088184072316096 1102 64-65 -0.6208097819435316 1102 66-67 -0.470549462648961 1102 68-69 -0.5804050895843744 1102 70-71 -0.6540685979161118 1102 72-73 -0.7610713384717052 1102 74-75 -0.6984665812259223 1102 76-77 -0.7488312345887742 1102 78-79 -1.0170905330399016 1102 80-81 -0.9702694978512127 1102 82-83 -0.7892162946296786 1102 84-85 -0.739339313677096 1102 86-87 -1.0887951687962492 1102 88-89 -0.9027781382216062 1102 90-91 -0.841650620596365 1102 92-93 -0.7922923143776295 1102 94-95 -0.832550189810668 1102 96-97 -0.918326377194024 1102 98-99 -1.0692340851814102 1102 100-101 -0.901230923568054 1103 1 0.29578332081466385 1103 2 0.23177603137276748 1103 3 0.25768740788032574 1103 4 0.0900821456902463 1103 5 0.18544804284407235 1103 6 0.22479092465015782 1103 7 0.21457184799274387 1103 8 0.21552662665956035 1103 9 0.1740763870432147 1103 10-11 0.3709290376002059 1103 12-13 0.29809929028150606 1103 14-15 0.3702930135169211 1103 16-17 0.3829603909614647 1103 18-19 0.36730615540461287 1103 20-21 0.19460316366724584 1103 22-23 0.2828119044084474 1103 24-25 0.18948240556922968 1103 26-27 0.27455071814889465 1103 28-29 0.34350194633351094 1103 30-31 0.3759328714108676 1103 32-33 0.26448913642833816 1103 34-35 0.47485693191095635 1103 36-37 0.6218746733361016 1103 38-39 0.5627177140381292 1103 40-41 0.6849147840449419 1103 42-43 0.7751684588234085 1103 44-45 0.59655858608442 1103 46-47 0.8610409142721522 1103 48-49 0.7307978034461868 1103 50-51 0.4971244669724726 1103 52-53 0.5208224385691196 1103 54-55 0.5849066640849614 1103 56-57 0.5049220250017186 1103 58-59 0.5077547833346259 1103 60-61 0.5199152848346529 1103 62-63 0.5375363128537956 1103 64-65 0.4643960959448563 1103 66-67 0.6093974581190267 1103 68-69 0.6544460290457472 1103 70-71 0.45603653226021024 1103 72-73 0.5329650824994907 1103 74-75 0.5660677758249513 1103 76-77 0.6572126488131964 1103 78-79 0.7629073124525263 1103 80-81 0.9328633520236771 1103 82-83 0.9349798047322935 1103 84-85 1.1282294714254633 1103 86-87 1.0224778024165921 1103 88-89 0.8676443900930266 1103 90-91 0.6942635516565154 1103 92-93 0.6557277421292262 1103 94-95 0.5405003480817356 1103 96-97 0.57511216792577 1103 98-99 0.43187254825361165 1103 100-101 0.26886744928906836 1104 1 0.34953121187274405 1104 2 0.2568517642448498 1104 3 0.2980231372552282 1104 4 0.2342416171433257 1104 5 0.28027363275707273 1104 6 0.13538596526944247 1104 7 0.21001133494684154 1104 8 0.28728499980877586 1104 9 0.38593466614661764 1104 10-11 0.28284733589084254 1104 12-13 0.25612130361805185 1104 14-15 0.3020365472765363 1104 16-17 0.2767949553970297 1104 18-19 0.30264375151801914 1104 20-21 0.2968224558189121 1104 22-23 0.364988568759415 1104 24-25 0.20430289148835357 1104 26-27 0.22880144712209471 1104 28-29 0.20757739092445604 1104 30-31 -0.02906904258684051 1104 32-33 0.13628673471565378 1104 34-35 -0.05297873838993894 1104 36-37 0.003967688898548261 1104 38-39 0.06850460339015996 1104 40-41 0.10314454883589264 1104 42-43 0.09229661160295421 1104 44-45 0.22827470950655737 1104 46-47 0.1417322218870325 1104 48-49 0.2858287818534535 1104 50-51 0.19053039044091946 1104 52-53 0.40391971218572564 1104 54-55 0.4090008233201132 1104 56-57 0.321629570878585 1104 58-59 0.15149837380528197 1104 60-61 0.15048064667474392 1104 62-63 0.13513129203611385 1104 64-65 0.20846624750511467 1104 66-67 0.34822183819643016 1104 68-69 0.20890280814206008 1104 70-71 0.21922303125122866 1104 72-73 0.2117939049419384 1104 74-75 0.442565301805697 1104 76-77 0.14005519532806332 1104 78-79 0.024335769108748906 1104 80-81 -0.05844331766757804 1104 82-83 -0.08107414637333576 1104 84-85 0.11459282853017427 1104 86-87 0.01756520831170505 1104 88-89 -0.10713101054686192 1104 90-91 -0.1664021414705772 1104 92-93 -0.04893768634633133 1104 94-95 0.08279969732251402 1104 96-97 0.3177669063697124 1104 98-99 -0.21954121079062006 1104 100-101 -0.10800342439181065 1105 1 -0.07340023020763198 1105 2 0.11306554999555374 1105 3 0.05170134612743027 1105 4 0.02037706422996166 1105 5 0.04850707270514931 1105 6 0.12408016635413333 1105 7 0.07610320904872481 1105 8 0.10442679742616434 1105 9 0.09940984345158199 1105 10-11 0.1601476650059368 1105 12-13 0.30864297422482423 1105 14-15 0.29259882419675165 1105 16-17 0.31814061895796186 1105 18-19 0.26264699631474997 1105 20-21 0.27570114677519797 1105 22-23 0.40730998905329585 1105 24-25 0.28711705734382065 1105 26-27 0.23251905709752663 1105 28-29 0.4009533701577581 1105 30-31 0.3898295806922647 1105 32-33 0.4177913933166906 1105 34-35 0.46846241490352725 1105 36-37 0.6379105804759888 1105 38-39 0.661222352428311 1105 40-41 0.5820348283519508 1105 42-43 0.47298080017200306 1105 44-45 0.2996231543218286 1105 46-47 0.5668539017646594 1105 48-49 0.7554435781265738 1105 50-51 0.7434437543680446 1105 52-53 0.8723385690901893 1105 54-55 0.9882758430206664 1105 56-57 1.0620296079619749 1105 58-59 0.9207284298939129 1105 60-61 0.7009094233863706 1105 62-63 0.592680079872757 1105 64-65 0.737530355417789 1105 66-67 0.6279256346086086 1105 68-69 0.7853386306980319 1105 70-71 0.7823945882901207 1105 72-73 0.9108992681274017 1105 74-75 0.8056302440946723 1105 76-77 1.0299546066292287 1105 78-79 0.9523105524026825 1105 80-81 0.961148301485995 1105 82-83 1.0799998813465024 1105 84-85 0.8961368882344338 1105 86-87 0.9765138941690275 1105 88-89 1.1796626667349273 1105 90-91 1.1266145924097337 1105 92-93 1.403039321951077 1105 94-95 1.3881188462586849 1105 96-97 1.512445439721585 1105 98-99 1.4281387595426658 1105 100-101 1.509669129263223 1106 1 0.12455984875705184 1106 2 0.10369946094093763 1106 3 0.007854011185557397 1106 4 0.2013597358684791 1106 5 0.06318134337854531 1106 6 0.05250745303980864 1106 7 0.08904464357384256 1106 8 -0.03240145438343944 1106 9 0.027859064772044917 1106 10-11 0.0289785489717147 1106 12-13 0.06658331201820289 1106 14-15 0.04133758269642129 1106 16-17 0.1144226983613379 1106 18-19 0.10475052779096217 1106 20-21 0.1275436560974228 1106 22-23 0.08666949771748023 1106 24-25 0.20673758816197818 1106 26-27 0.12091563529449445 1106 28-29 -0.12624557697977679 1106 30-31 -0.057571165085320786 1106 32-33 -0.007389567420318599 1106 34-35 0.018813369060140417 1106 36-37 0.08358945742268276 1106 38-39 0.056791556201062576 1106 40-41 -0.08953788496237536 1106 42-43 -0.16759827418641038 1106 44-45 6.979514264600084E-4 1106 46-47 0.11661262078197865 1106 48-49 0.15737382598794625 1106 50-51 0.06046503096379752 1106 52-53 0.06185381366136511 1106 54-55 0.2145754701318765 1106 56-57 0.19579797821861433 1106 58-59 -0.05356417813415959 1106 60-61 0.2989791755249982 1106 62-63 0.5652617863873459 1106 64-65 0.4059664159573799 1106 66-67 0.46827439530174786 1106 68-69 0.48238842231900847 1106 70-71 0.11040511691481925 1106 72-73 0.32886373401904123 1106 74-75 0.27483401685185527 1106 76-77 0.47886884220720916 1106 78-79 0.7011589840762937 1106 80-81 0.8382595100843702 1106 82-83 0.8688461195566362 1106 84-85 0.9150555107393714 1106 86-87 0.800949077323228 1106 88-89 0.7172586307621245 1106 90-91 0.7036380624689578 1106 92-93 0.44706213397125794 1106 94-95 0.2807378416524102 1106 96-97 0.4028600043190842 1106 98-99 0.41122715612086225 1106 100-101 0.31898038173087073 1107 1 0.16532664070280134 1107 2 0.21416621299334793 1107 3 0.19106521491453776 1107 4 0.35375228631254885 1107 5 0.3567837117830237 1107 6 0.3440437664754654 1107 7 0.34980429671176694 1107 8 0.3661245917668481 1107 9 0.37846691326134874 1107 10-11 0.45533334772032674 1107 12-13 0.4804179016417365 1107 14-15 0.5117304270914147 1107 16-17 0.4826529039170069 1107 18-19 0.5978573789468129 1107 20-21 0.5188805028440129 1107 22-23 0.5643061323994871 1107 24-25 0.5621116406952069 1107 26-27 0.4343953841764332 1107 28-29 0.3136456611834504 1107 30-31 0.3961735595456659 1107 32-33 0.3656572337638835 1107 34-35 0.3448133270671505 1107 36-37 0.17439929218028283 1107 38-39 0.37566530428513545 1107 40-41 0.42928967083580716 1107 42-43 0.5533205323729007 1107 44-45 0.5723925784237522 1107 46-47 0.5686587290818963 1107 48-49 0.4713511917678588 1107 50-51 0.5437644199658322 1107 52-53 0.6560062901977872 1107 54-55 0.5963358161540881 1107 56-57 0.412189940760868 1107 58-59 0.48805087215243503 1107 60-61 0.502720750681938 1107 62-63 0.548857225948943 1107 64-65 0.44724132304789066 1107 66-67 0.15663603890726563 1107 68-69 0.027287324202386998 1107 70-71 0.07408328266113884 1107 72-73 0.15671978205902093 1107 74-75 0.05958006426193663 1107 76-77 0.19617205257107528 1107 78-79 0.44939422879042823 1107 80-81 0.4718580991200021 1107 82-83 0.4615204429446287 1107 84-85 0.3275946167086765 1107 86-87 0.29095684602585337 1107 88-89 0.23287582269107077 1107 90-91 0.3881195540598661 1107 92-93 0.5785820701419162 1107 94-95 0.30886894375835894 1107 96-97 0.5839148681568247 1107 98-99 0.44202271326281917 1107 100-101 0.031099557828461855 1108 1 -0.08227184867282489 1108 2 0.06571769251902282 1108 3 0.16131615905477048 1108 4 0.005483447208682435 1108 5 0.06868374064586646 1108 6 0.12047461150638128 1108 7 0.04065070979191887 1108 8 0.09428537813708715 1108 9 0.36797059429032686 1108 10-11 0.27230632640654306 1108 12-13 0.32176778842620735 1108 14-15 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2316 68-69 -0.6113313511866671 2316 70-71 -0.6789878576303856 2316 72-73 -0.7486503358224432 2316 74-75 -0.4248389776578989 2316 76-77 -0.6718462671675276 2316 78-79 -0.8643057939902441 2316 80-81 -0.8331240673556941 2316 82-83 -1.0776334081785954 2316 84-85 -0.8970023864432193 2316 86-87 -0.7269599807288536 2316 88-89 -0.6630539176432961 2316 90-91 -0.9532436820192771 2316 92-93 -0.8251423993574853 2316 94-95 -0.4964991340631286 2316 96-97 -0.4584741780811328 2316 98-99 -0.5098216709620722 2316 100-101 -0.4174807304320147 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2246.0 3 340.0 4 274.0 5 301.0 6 286.0 7 338.0 8 430.0 9 451.0 10 537.0 11 869.0 12 1582.0 13 3163.0 14 2897.0 15 3437.0 16 4803.0 17 7734.0 18 7101.0 19 3827.0 20 3878.0 21 3766.0 22 3895.0 23 4369.0 24 4672.0 25 5254.0 26 5705.0 27 6344.0 28 7407.0 29 8288.0 30 9622.0 31 11208.0 32 14216.0 33 18957.0 34 30492.0 35 100137.0 36 78644.0 37 64459.0 38 67608.0 39 12561.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.41015449452229 21.684759270170673 15.495790598503989 12.409295636803051 2 15.615860478190747 27.18705579556578 39.239312678055576 17.957771048187894 3 19.507537247678624 38.004991607560775 29.368553669748547 13.11891747501206 4 9.988058189908672 22.155347199655502 37.577228024776765 30.279366585659062 5 8.787174860173675 44.89078093377067 30.167990987308457 16.154053218747197 6 20.78026128289703 40.96600930733127 19.3399168917101 18.913812518061604 7 20.704019580681617 44.579934186267614 20.39189267299026 14.324153560060513 8 25.74194667192891 32.20773111521726 20.457974793048255 21.592347419805577 9 29.421858868830046 20.650887102238983 21.577052931164374 28.350201097766597 10-11 21.99270148640946 33.98641942337358 23.2491140935852 20.771764996631763 12-13 26.65944504458538 34.257993279307364 26.307166745725823 12.775394930381436 14-15 22.853939638548816 32.51077527143719 31.691505847297584 12.943779242716417 16-17 13.365705209327725 37.72502705406628 31.11506597604893 17.794201760557065 18-19 12.714628684527602 33.193411725311165 40.5803557636441 13.511603826517138 20-21 15.552288640271097 31.575112750105898 40.49644930605736 12.376149303565644 22-23 13.974582606528783 32.87415898330426 40.75873411412908 12.392524296037877 24-25 13.3613256055522 33.968243524793294 40.99432378924451 11.676107080409995 26-27 12.675163362857441 33.81798063072268 42.04887677132191 11.457979235097968 28-29 11.71599626788306 35.39470326480486 41.56386066763425 11.325439799677826 30-31 11.567566597758113 36.30178358195566 40.569362738746214 11.561287081540017 32-33 11.258531606812527 37.00026312432226 40.26489841806468 11.476306850800535 34-35 11.886930199252367 36.62993749082437 39.8346937409929 11.648438568930374 36-37 11.844324025899988 36.528825439117625 40.199090659961634 11.427759875020753 38-39 11.73327360573385 36.95595222858606 40.130247496240365 11.180526669439733 40-41 11.006401848978744 37.422126165608354 40.454507285552054 11.116964699860848 42-43 10.90632365573441 38.05733359866481 38.97832715170994 12.058015593890843 44-45 11.237763028018653 37.6431209441357 38.67128160561473 12.447834422230912 46-47 11.660969282129548 38.02179074563395 38.320128336170356 11.997111636066144 48-49 11.672824823607312 37.92787854660828 38.0862168644812 12.313079765303204 50-51 11.418398575800612 38.28375972636169 38.328766027243816 11.969075670593883 52-53 11.244876690517101 38.786128208256905 37.616443398056894 12.352551703169102 54-55 10.765576795187831 38.13043456520978 38.12283429920047 12.981154340401915 56-57 11.83698648352758 38.45650863288565 36.916996521650454 12.789508361936319 58-59 11.857245244656893 37.66979441244061 37.53204964809112 12.94091069481138 60-61 11.598738563737305 37.89494825952972 38.101490461287575 12.4048227154454 62-63 11.854338274849065 37.98697727622693 37.93158270489492 12.227101744029085 64-65 11.615136636016375 38.259484903073 37.952515220955156 12.17286323995547 66-67 11.531593894391255 37.96018890214881 37.37299571559043 13.135221487869508 68-69 11.193882022438187 38.07738713375873 37.763644694691386 12.965086149111697 70-71 12.012220497142948 37.81681658850457 37.13206459977272 13.038898314579766 72-73 11.662183776170895 37.13523791995215 37.67278467021497 13.52979363366199 74-75 11.393193933579882 38.14510231907225 37.18848376509378 13.273219982254092 76-77 11.70703460194462 37.05702450079309 37.57928403838037 13.656656858881913 78-79 11.445532555679321 36.683058328050535 38.13061067771088 13.740798438559269 80-81 11.978137574094665 37.32551256309546 37.096529049908405 13.59982081290147 82-83 11.707732217539748 36.618877419380645 37.34071639868553 14.332673964394077 84-85 11.726656692774796 36.19242677589945 37.116278511596654 14.964638019729104 86-87 12.20677419612926 36.22308763171159 37.26847962620711 14.301658545952044 88-89 12.332040400322466 35.462305234878386 37.224868322874514 14.980786041924638 90-91 12.271301738151807 34.91289233462548 37.76446167312743 15.051344254095286 92-93 12.728558581718916 35.42061581151738 37.53677393669431 14.314051670069391 94-95 12.369067328228239 34.84442132535725 37.61327658042025 15.173234765994268 96-97 12.247274298730257 34.32177830062692 37.79509048657982 15.635856914063009 98-99 12.82376241585228 34.26385123983475 37.138227321376775 15.774159022936193 100-101 13.059349952893687 33.01492399981598 38.40654647722795 15.519179570062388 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 20289.0 1 12518.0 2 7238.5 3 9468.0 4 7527.5 5 6934.5 6 11516.5 7 20612.0 8 28460.0 9 26857.5 10 17142.0 11 9975.5 12 8489.5 13 7783.5 14 6737.5 15 5593.0 16 4486.0 17 3699.5 18 3204.5 19 2885.5 20 2688.5 21 2594.0 22 2571.5 23 2594.5 24 2639.5 25 2815.0 26 3136.5 27 3483.0 28 3643.0 29 3989.5 30 4813.0 31 5567.0 32 6728.0 33 8016.5 34 8913.5 35 9792.5 36 11366.5 37 12602.5 38 12144.5 39 11222.0 40 10157.5 41 9082.0 42 7905.0 43 7978.5 44 8410.0 45 7940.5 46 7873.0 47 10189.0 48 20538.5 49 20198.5 50 10418.5 51 7197.0 52 4796.0 53 3965.5 54 3721.0 55 3626.5 56 3412.5 57 3056.5 58 2852.0 59 2596.5 60 2186.0 61 1932.0 62 1683.5 63 1397.5 64 1104.0 65 938.5 66 807.5 67 681.5 68 547.5 69 400.0 70 344.5 71 280.0 72 238.5 73 230.0 74 187.0 75 142.0 76 111.0 77 88.5 78 71.5 79 53.5 80 39.5 81 32.5 82 27.5 83 21.0 84 13.5 85 8.0 86 5.0 87 5.5 88 6.0 89 4.0 90 1.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.5 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28579964150567616 2 0.09978092013543118 3 0.09121688906592312 4 0.09978092013543118 5 0.11651065524795857 6 0.0687114120693089 7 0.07588129854610635 8 0.08225453096992631 9 0.07050388368850827 10-11 0.07050388368850827 12-13 0.07289384584744074 14-15 0.07418840868352918 16-17 0.06512646883091018 18-19 0.07777335192192791 20-21 0.08713403704441347 22-23 0.09460266879107748 24-25 0.10615415255925115 26-27 0.10605457080262895 28-29 0.10117506472814179 30-31 0.09340768771161123 32-33 0.0868352917745469 34-35 0.2894841665006971 36-37 0.43357896833300136 38-39 0.4086835291774547 40-41 0.38478390758812986 42-43 0.41754630551682936 44-45 0.42411870145389363 46-47 0.4307906791475802 48-49 0.4423421629157538 50-51 0.43218482374029077 52-53 0.43397729535949015 54-55 0.42172873929496113 56-57 0.3996215893248357 58-59 0.3783110934076877 60-61 0.3421629157538339 62-63 0.4084843656642103 64-65 0.44084843656642103 66-67 0.45100577574188405 68-69 0.46345349531965746 70-71 0.4548894642501493 72-73 0.4454291973710416 74-75 0.45140410276837284 76-77 0.4295956980681139 78-79 0.4080860386377215 80-81 0.4110734913363872 82-83 0.42362079267078273 84-85 0.4238199561840271 86-87 0.4270065723959371 88-89 0.4387572196773551 90-91 0.4555865365465047 92-93 0.44921330412268473 94-95 0.45259908384783903 96-97 0.4529974108743278 98-99 0.4451304521011751 100-101 0.43158733320055764 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 502100.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.436887713517947 #Duplication Level Percentage of deduplicated Percentage of total 1 70.10104610012547 18.532534843491625 2 12.72311334646821 6.7271903781388325 3 5.391488513927774 4.276025293542909 4 2.94080966467803 3.109834195676858 5 1.8071838091742933 2.3888157720414216 6 1.2227588854589768 1.9395563613351194 7 0.8872842103845421 1.6419923127939506 8 0.6513447632951785 1.377562269601805 9 0.497686526985537 1.1841554547402624 >10 3.3150064688027814 16.7619542745027 >50 0.29588785065926093 5.402997358073916 >100 0.13401536238511055 6.320382321174402 >500 0.016563696474564227 3.1773087797728463 >1k 0.010540534120177235 6.102829323849857 >5k 0.002258685882895122 4.272040239319966 >10k+ 0.0030115811771934957 16.784820821943534 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27919 5.560446126269667 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 21892 4.360087631945827 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18546 3.6936865166301533 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15971 3.180840470025891 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7409 1.4756024696275643 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7114 1.416849233220474 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6940 1.382194781915953 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4816 0.9591714797849035 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3482 0.693487353116909 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3223 0.6419040031866162 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2946 0.5867357100179247 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2339 0.4658434574785899 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2133 0.4248157737502489 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1895 0.377414857598088 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1850 0.36845249950209125 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1600 0.3186616211909978 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1569 0.31248755228042224 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1287 0.25632344154550885 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1205 0.2399920334594702 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1174 0.23381796454889464 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1142 0.22744473212507466 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 943 0.18781119298944435 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 897 0.17864967138020313 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 892 0.17765385381398127 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 881 0.17546305516829316 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 876 0.1744672376020713 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 843 0.16789484166500696 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 838 0.1668990240987851 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 813 0.16191993626767576 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 793 0.15793666600278827 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 789 0.15714001194981078 No Hit GTACATGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 739 0.1471818362875921 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 697 0.13881696873132843 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 687 0.13682533359888469 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 676 0.13463453495319658 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 672 0.13383788090021909 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 667 0.1328420633339972 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 644 0.12826130252937662 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 559 0.11133240390360487 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 528 0.10515833499302926 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 514 0.10237004580760806 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 513 0.10217088229436368 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.9916351324437363E-4 0.0 16-17 0.0 0.0 0.0 4.979087831109341E-4 0.0 18-19 0.0 0.0 0.0 5.974905397331209E-4 0.0 20-21 0.0 0.0 0.0 9.958175662218682E-4 0.0 22-23 0.0 0.0 0.0 0.003186616211909978 0.0 24-25 0.0 0.0 0.0 0.005775741884086835 0.0 26-27 0.0 0.0 0.0 0.013343955387373034 0.0 28-29 0.0 0.0 0.0 0.026190001991635133 0.0 30-31 0.0 0.0 0.0 0.059350726946823334 0.0 32-33 0.0 0.0 0.0 0.09619597689703246 0.0 34-35 0.0 0.0 0.0 0.14160525791674966 0.0 36-37 0.0 0.0 0.0 0.20513841864170484 0.0 38-39 0.0 9.958175662218681E-5 0.0 0.28589922326229833 0.0 40-41 0.0 1.9916351324437363E-4 0.0 0.38040231029675364 0.0 42-43 0.0 1.9916351324437363E-4 0.0 0.4659430392352121 0.0 44-45 0.0 1.9916351324437363E-4 0.0 0.5606452897829117 0.0 46-47 0.0 1.9916351324437363E-4 0.0 0.6722764389563831 0.0 48-49 0.0 1.9916351324437363E-4 0.0 0.7977494523003386 0.0 50-51 0.0 1.9916351324437363E-4 0.0 0.938159729137622 0.0 52-53 0.0 1.9916351324437363E-4 0.0 1.0601473809998008 0.0 54-55 0.0 1.9916351324437363E-4 0.0 1.1935869348735313 0.0 56-57 0.0 1.9916351324437363E-4 0.0 1.3485361481776539 0.0 58-59 0.0 1.9916351324437363E-4 0.0 1.5131447918741285 0.0 60-61 0.0 1.9916351324437363E-4 0.0 1.723859788886676 0.0 62-63 0.0 1.9916351324437363E-4 0.0 1.9234216291575383 0.0 64-65 0.0 1.9916351324437363E-4 0.0 2.1083449512049395 0.0 66-67 0.0 1.9916351324437363E-4 0.0 2.314578769169488 0.0 68-69 0.0 1.9916351324437363E-4 0.0 2.548396733718383 0.0 70-71 0.0 1.9916351324437363E-4 0.0 2.8133837880900217 0.0 72-73 0.0 1.9916351324437363E-4 0.0 3.0874327823142798 0.0 74-75 0.0 1.9916351324437363E-4 0.0 3.3631746664011155 0.0 76-77 0.0 1.9916351324437363E-4 0.0 3.637721569408485 0.0 78-79 0.0 1.9916351324437363E-4 0.0 3.980979884485162 0.0 80-81 0.0 1.9916351324437363E-4 0.0 4.335391356303525 0.0 82-83 0.0 1.9916351324437363E-4 0.0 4.689504082852022 0.0 84-85 0.0 1.9916351324437363E-4 0.0 5.084744074885481 0.0 86-87 0.0 1.9916351324437363E-4 0.0 5.508564031069508 0.0 88-89 0.0 1.9916351324437363E-4 0.0 5.905596494722167 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATAG 25 0.0050669345 56.865074 2 GATTGTC 25 0.0050669345 56.865074 2 GTATATG 35 2.8026022E-4 54.2221 1 GTCCAAG 65 4.4536137E-8 51.093903 1 GGAGTAT 60 8.410425E-5 39.481762 8 GGTAAGC 50 0.0016354598 37.902493 7 CTATGTC 55 0.0026083812 34.463684 3 CTCTACA 55 0.0026134881 34.449944 9 GCCTTTG 70 2.082886E-4 33.841515 7 GATGACC 130 8.4928615E-9 32.793694 9 GGGAACG 75 3.122695E-4 31.585411 7 GATATTA 60 0.00400273 31.579111 9 AAGACTT 95 3.543822E-5 29.92899 5 TACAGCG 40 4.4715375E-4 29.712042 78-79 CAAGACT 80 4.5528155E-4 29.617228 4 GGACAAT 80 4.5581366E-4 29.61132 6 GTATGGT 65 0.0058745253 29.196516 1 TAGTCAA 65 0.0059205294 29.14995 9 CATGGGC 585 0.0 27.54149 4 GGGAGAT 155 4.692083E-8 27.509874 7 >>END_MODULE