##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939081_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 502100 Sequences flagged as poor quality 0 Sequence length 101 %GC 27 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.004668392750446 33.0 31.0 34.0 30.0 34.0 2 32.141431985660226 33.0 31.0 34.0 30.0 34.0 3 32.11164708225453 33.0 31.0 34.0 30.0 34.0 4 35.81454291973711 37.0 35.0 37.0 35.0 37.0 5 35.825919139613625 37.0 35.0 37.0 35.0 37.0 6 35.963318064130654 37.0 35.0 37.0 35.0 37.0 7 35.89882294363672 37.0 35.0 37.0 35.0 37.0 8 35.9335849432384 37.0 35.0 37.0 35.0 37.0 9 37.48130252937662 39.0 37.0 39.0 35.0 39.0 10-11 37.43296952798247 39.0 37.0 39.0 34.0 39.0 12-13 37.31003087034455 39.0 37.0 39.0 34.0 39.0 14-15 38.21496614220275 40.0 37.5 41.0 33.0 41.0 16-17 38.21431089424418 40.0 37.5 41.0 33.5 41.0 18-19 38.38985759808803 40.0 37.5 41.0 34.0 41.0 20-21 38.458782115116506 40.0 37.5 41.0 34.0 41.0 22-23 38.24721868153755 40.0 37.0 41.0 34.0 41.0 24-25 38.042043417645885 40.0 36.0 41.0 33.5 41.0 26-27 37.694336785500894 40.0 35.5 41.0 33.0 41.0 28-29 37.31589523999203 39.0 35.0 41.0 33.0 41.0 30-31 36.65636028679546 37.5 35.0 40.0 33.0 41.0 32-33 35.89021011750647 36.0 35.0 40.0 31.5 41.0 34-35 35.43206632144991 35.0 35.0 40.0 31.0 41.0 36-37 35.110687114120694 35.0 35.0 40.0 30.0 41.0 38-39 34.80668392750448 35.0 35.0 40.0 29.5 41.0 40-41 34.47842760406294 35.0 35.0 40.0 27.5 41.0 42-43 34.13804023102968 35.0 34.0 40.0 25.0 41.0 44-45 33.65960565624378 35.0 33.0 39.0 23.5 41.0 46-47 33.03301931886078 35.0 33.0 39.0 21.0 41.0 48-49 32.51527683728341 35.0 33.0 39.0 20.0 41.0 50-51 32.28319159529974 35.0 33.0 39.0 19.5 40.5 52-53 32.08899721171082 35.0 33.0 38.0 16.5 40.0 54-55 31.884872535351523 35.0 33.0 38.0 14.0 40.0 56-57 31.584806811392156 35.0 33.0 38.0 9.0 40.0 58-59 31.272550288787095 35.0 32.0 38.0 4.5 40.0 60-61 30.864101772555266 35.0 31.0 37.0 2.0 40.0 62-63 30.41042919737104 35.0 31.0 36.5 2.0 40.0 64-65 29.956958773152756 35.0 30.0 36.0 2.0 39.0 66-67 29.58665504879506 35.0 29.0 35.5 2.0 39.0 68-69 29.110065723959373 35.0 29.0 35.0 2.0 39.0 70-71 28.748521210914163 34.0 28.5 35.0 2.0 38.0 72-73 28.322881896036648 34.0 27.5 35.0 2.0 37.0 74-75 27.84510555666202 34.0 26.5 35.0 2.0 37.0 76-77 25.940141406094405 31.5 24.0 34.0 2.0 35.0 78-79 26.971339374626567 33.0 26.0 35.0 2.0 36.0 80-81 26.973128858793068 33.0 26.0 35.0 2.0 35.5 82-83 26.63109141605258 33.0 24.5 35.0 2.0 35.0 84-85 26.15427703644692 33.0 23.5 35.0 2.0 35.0 86-87 25.467189802828123 33.0 19.0 35.0 2.0 35.0 88-89 24.74975801633141 33.0 11.0 35.0 2.0 35.0 90-91 24.10480979884485 32.0 2.0 35.0 2.0 35.0 92-93 23.675525791674964 32.0 2.0 35.0 2.0 35.0 94-95 23.2067187811193 31.5 2.0 35.0 2.0 35.0 96-97 22.458434574785898 31.0 2.0 35.0 2.0 35.0 98-99 21.587728540131447 31.0 2.0 34.5 2.0 35.0 100-101 18.49970025891257 25.5 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2657201320978828 1101 2 0.20816316104477295 1101 3 0.3035666473697418 1101 4 0.2565685743788606 1101 5 0.17003366303540446 1101 6 0.24223330000646115 1101 7 0.020709871766158017 1101 8 0.18050405858278395 1101 9 0.2925726519268963 1101 10-11 0.29977889661287094 1101 12-13 0.26063101733003435 1101 14-15 0.41269235275787963 1101 16-17 0.43352136428008237 1101 18-19 0.5097968303662768 1101 20-21 0.5089162936473528 1101 22-23 0.5131460500360845 1101 24-25 0.46957610121744864 1101 26-27 0.41380964808094944 1101 28-29 0.2006501733700432 1101 30-31 0.4486490578659428 1101 32-33 0.3887898315337637 1101 34-35 0.2738651137644226 1101 36-37 0.2786826380444438 1101 38-39 0.38062680272080485 1101 40-41 0.5005395281217986 1101 42-43 0.7124143412743464 1101 44-45 1.0698168454350565 1101 46-47 1.2800368082781972 1101 48-49 0.45135293157991185 1101 50-51 0.7228385177028969 1101 52-53 0.4479972456503134 1101 54-55 0.5146312861744242 1101 56-57 0.7091621880879266 1101 58-59 0.9340699792053577 1101 60-61 1.0742265495033685 1101 62-63 0.791044561445382 1101 64-65 0.6742141859668749 1101 66-67 0.7893614627132521 1101 68-69 0.6294304666464718 1101 70-71 0.9753076764583142 1101 72-73 0.963285808503894 1101 74-75 0.6408871912479022 1101 76-77 0.7912924383501654 1101 78-79 0.8876702496797506 1101 80-81 1.0559245520434608 1101 82-83 0.9521614971291612 1101 84-85 0.8156433866348038 1101 86-87 0.8525096158976488 1101 88-89 1.1640597847076997 1101 90-91 1.2705303975653344 1101 92-93 0.8220097207986043 1101 94-95 0.8514816164936327 1101 96-97 0.880949160070962 1101 98-99 0.42894438731216766 1101 100-101 0.4134386192095185 1102 1 0.07158920658998369 1102 2 0.043377607997371115 1102 3 -0.019232449695721243 1102 4 0.05566563984161377 1102 5 0.07296820028145135 1102 6 0.14516783725250804 1102 7 0.029739217138619267 1102 8 0.09021060485817856 1102 9 0.008148272694391778 1102 10-11 0.11467731647743307 1102 12-13 -0.022664693730909846 1102 14-15 0.02443050174208139 1102 16-17 0.1648983394493797 1102 18-19 0.012054166709390302 1102 20-21 0.2504512823606646 1102 22-23 0.1948616256568485 1102 24-25 0.269801834851755 1102 26-27 0.351732898645281 1102 28-29 0.3372190221285081 1102 30-31 0.22291542355443283 1102 32-33 0.1912729015111907 1102 34-35 -0.004915924610294553 1102 36-37 0.06875035813473573 1102 38-39 0.20455456795782823 1102 40-41 -0.0017178082213149537 1102 42-43 -0.09571206956086797 1102 44-45 -0.43921249993741185 1102 46-47 -0.05743497501751449 1102 48-49 -0.2732520345600449 1102 50-51 -0.2816761549833373 1102 52-53 -0.2021156211668398 1102 54-55 -0.32058316077817395 1102 56-57 -0.5560748322280986 1102 58-59 -0.5106252803883216 1102 60-61 -0.7677599064785738 1102 62-63 -0.6480073572905098 1102 64-65 -0.7219483422498243 1102 66-67 -0.29190264563889556 1102 68-69 -0.6865966213896435 1102 70-71 -0.9818029330224967 1102 72-73 -1.1969850718572808 1102 74-75 -0.9866523121381015 1102 76-77 -0.4197685097310959 1102 78-79 -1.0197789602525305 1102 80-81 -1.0750009558572167 1102 82-83 -0.8266195412455559 1102 84-85 -0.8920315569317836 1102 86-87 -0.7005377881655548 1102 88-89 -0.4736377322223255 1102 90-91 -0.36942445570780436 1102 92-93 -0.28972842818559386 1102 94-95 -0.5841842977275853 1102 96-97 -0.7127302981683172 1102 98-99 -0.9344867639293675 1102 100-101 -0.8179383559597824 1103 1 0.1484120913776863 1103 2 0.20473063875296305 1103 3 0.18097026988043297 1103 4 -0.05219684381595613 1103 5 0.04656568930080596 1103 6 0.032653575881361974 1103 7 0.1844916201490534 1103 8 0.030320190947897174 1103 9 -0.007080178557430372 1103 10-11 0.25203451914314456 1103 12-13 0.10869171568429437 1103 14-15 0.2584173690391012 1103 16-17 0.100711151914183 1103 18-19 0.23488900901412535 1103 20-21 0.15123620864223852 1103 22-23 0.24075753356144247 1103 24-25 0.21697967476205093 1103 26-27 0.23190800135164125 1103 28-29 0.3606688784130583 1103 30-31 0.2623208773965544 1103 32-33 0.22387451837119698 1103 34-35 0.1643598180827368 1103 36-37 0.3859061143424114 1103 38-39 0.28687879196785104 1103 40-41 0.6921609419684245 1103 42-43 0.570065046630738 1103 44-45 0.515239251334485 1103 46-47 0.5013634952821064 1103 48-49 0.3637305775845192 1103 50-51 0.5309012726325548 1103 52-53 0.1301412744815451 1103 54-55 0.4741387853297603 1103 56-57 0.5877997558447454 1103 58-59 0.506434502300209 1103 60-61 0.37439597214430265 1103 62-63 0.44056493868006896 1103 64-65 0.42461938028876745 1103 66-67 0.5993094117689388 1103 68-69 0.620415811098372 1103 70-71 0.430342124096434 1103 72-73 0.2915642100551388 1103 74-75 0.15278350067436364 1103 76-77 0.09458834527543658 1103 78-79 0.6037502210835584 1103 80-81 0.7546974620486999 1103 82-83 1.1336787601210858 1103 84-85 1.1166231979195302 1103 86-87 1.099301113427309 1103 88-89 0.8219680516511865 1103 90-91 0.8516525042820113 1103 92-93 0.5691831537615926 1103 94-95 0.42623078774270695 1103 96-97 0.7707926155093787 1103 98-99 -0.08881561033746266 1103 100-101 -0.048867291486143216 1104 1 0.17963152058104015 1104 2 0.0930193357694904 1104 3 0.05371541517884992 1104 4 -0.07718278420319535 1104 5 0.04818667994820913 1104 6 0.1328336334430702 1104 7 0.19137728596032844 1104 8 -0.03515270826240169 1104 9 0.30658387467534 1104 10-11 0.2507396169933216 1104 12-13 0.25328360884068246 1104 14-15 0.17885886450930144 1104 16-17 0.09343323472175769 1104 18-19 0.295354795714438 1104 20-21 0.13101163291172924 1104 22-23 0.02063361993236157 1104 24-25 -0.10318488178122465 1104 26-27 0.17286235368789704 1104 28-29 0.3223274873856923 1104 30-31 0.14335292386180498 1104 32-33 -0.05764146232814937 1104 34-35 0.1474173939692065 1104 36-37 -0.1369697026510508 1104 38-39 -0.3400806927178053 1104 40-41 -0.37232423990861463 1104 42-43 -0.2339628552024422 1104 44-45 -0.16784526229743335 1104 46-47 -0.07114866214774906 1104 48-49 -0.41965923049585285 1104 50-51 -0.24844314770969333 1104 52-53 -0.19863863468626874 1104 54-55 -0.33669680463049545 1104 56-57 -0.16159178192244994 1104 58-59 -0.2305680355189672 1104 60-61 0.2809621657463488 1104 62-63 -0.1644308316213028 1104 64-65 -0.13648676860607623 1104 66-67 -0.28069711000101094 1104 68-69 -0.3062379065467944 1104 70-71 -0.283063893890418 1104 72-73 -0.17271255873866664 1104 74-75 -0.16718020425808078 1104 76-77 -0.10653973188938792 1104 78-79 -0.1561683040512456 1104 80-81 0.03560852495542477 1104 82-83 0.2764015518166971 1104 84-85 0.3138311242329834 1104 86-87 0.448168546638243 1104 88-89 0.5443225996405445 1104 90-91 0.08345180390062623 1104 92-93 0.3533763682852893 1104 94-95 0.5224268945296053 1104 96-97 0.44043661193658323 1104 98-99 0.35535473073220203 1104 100-101 -0.06017169273567902 1105 1 0.2154679828255155 1105 2 0.187155524523952 1105 3 0.3327679345317449 1105 4 0.14803392186457387 1105 5 0.1511440804024815 1105 6 0.08388355695857541 1105 7 0.20210365688694765 1105 8 0.12230800651996532 1105 9 0.21127492367176615 1105 10-11 0.09921456252708793 1105 12-13 0.2993203213980493 1105 14-15 0.25432820118015087 1105 16-17 0.19333357310770793 1105 18-19 0.11224628044072205 1105 20-21 0.2597976153898216 1105 22-23 0.23579145611648045 1105 24-25 0.07134114612403408 1105 26-27 0.15522456571218868 1105 28-29 0.11320960855567108 1105 30-31 0.37504068170725446 1105 32-33 0.37115379099777357 1105 34-35 0.6228589180965898 1105 36-37 0.5433529228530531 1105 38-39 0.6549315911555595 1105 40-41 0.2606591189195484 1105 42-43 0.7231919216018952 1105 44-45 0.7629223639019855 1105 46-47 0.47162702968927306 1105 48-49 0.6474362128824396 1105 50-51 0.24789254968982988 1105 52-53 0.4340665987073251 1105 54-55 0.3002683833359434 1105 56-57 0.5380512904365169 1105 58-59 0.5197935275459749 1105 60-61 0.4334119872825326 1105 62-63 -0.006522798431667809 1105 64-65 0.19103374314472887 1105 66-67 0.21399764733454774 1105 68-69 0.1736142234304907 1105 70-71 0.10808708186631577 1105 72-73 0.11755073333939592 1105 74-75 0.3887091018189608 1105 76-77 0.7013543950285204 1105 78-79 0.7284559948408855 1105 80-81 0.39624682282776647 1105 82-83 0.24901083193536522 1105 84-85 0.40421261962073274 1105 86-87 0.3608209361992678 1105 88-89 0.9011737561788102 1105 90-91 0.8824366460644448 1105 92-93 0.8787553141898918 1105 94-95 -0.08056295389203427 1105 96-97 0.004516712061743533 1105 98-99 -0.27378122625106016 1105 100-101 -0.3906774174842056 1106 1 0.024790202610553536 1106 2 -0.09773151210688269 1106 3 0.05090787750169312 1106 4 0.033622866808642016 1106 5 0.03434215015462172 1106 6 -0.02052530463387825 1106 7 0.14959214120368358 1106 8 0.041917570020729045 1106 9 -0.045557715064795445 1106 10-11 0.0914826037641987 1106 12-13 -0.065353949275341 1106 14-15 0.120702406049638 1106 16-17 -0.25374912046544296 1106 18-19 -0.34252450262574285 1106 20-21 -0.008770052958503527 1106 22-23 0.07156684545625325 1106 24-25 0.007631999381324306 1106 26-27 0.20040991337614855 1106 28-29 0.09197688208056576 1106 30-31 0.003095079601528994 1106 32-33 0.12509105794227793 1106 34-35 0.11759460574738512 1106 36-37 0.4096174545485951 1106 38-39 0.5081011458824705 1106 40-41 0.5018194610998563 1106 42-43 0.7320973833259572 1106 44-45 0.7697330680037595 1106 46-47 1.1083675429597264 1106 48-49 1.0666399856396254 1106 50-51 1.1693117205606356 1106 52-53 1.344408779010024 1106 54-55 1.0990510111051925 1106 56-57 1.0252195290853443 1106 58-59 0.8588262101518076 1106 60-61 0.760238045628661 1106 62-63 0.6824550475871973 1106 64-65 0.7474496238203159 1106 66-67 1.0180226528182068 1106 68-69 1.0173409178810253 1106 70-71 1.1302191283677416 1106 72-73 1.18586615338765 1106 74-75 1.2081651228762098 1106 76-77 1.052966590685461 1106 78-79 1.314330090037199 1106 80-81 1.2974641950585166 1106 82-83 1.3231279628851347 1106 84-85 1.3470948306319208 1106 86-87 1.1414416863637769 1106 88-89 1.2885065045885504 1106 90-91 1.1449503552111864 1106 92-93 1.7086960906759607 1106 94-95 0.9368531461299021 1106 96-97 1.1472892498989857 1106 98-99 0.9431632791229241 1106 100-101 0.8836371251786588 1107 1 0.14648587943252522 1107 2 0.05021398099576402 1107 3 0.2729248006755256 1107 4 0.049693582999040586 1107 5 0.21731819315544243 1107 6 0.12483342045903356 1107 7 0.12256125536161733 1107 8 0.2152100301798825 1107 9 0.25447377967557827 1107 10-11 0.22029399226232016 1107 12-13 0.1952188350696673 1107 14-15 0.45955851707909545 1107 16-17 0.4817874449281305 1107 18-19 0.3126752790107261 1107 20-21 0.3941001677146474 1107 22-23 0.4404584405598655 1107 24-25 0.36226067639226045 1107 26-27 0.29612242938597433 1107 28-29 0.20584296644448585 1107 30-31 0.0700486983472004 1107 32-33 0.18668007257682717 1107 34-35 0.14022252688455694 1107 36-37 0.41849267238721666 1107 38-39 0.48772614850703633 1107 40-41 0.5159501012829395 1107 42-43 0.4076049735583851 1107 44-45 0.1263123192688056 1107 46-47 0.20451258673741535 1107 48-49 0.27236362979105166 1107 50-51 0.4764946210162577 1107 52-53 0.6182261515088143 1107 54-55 0.7635543390494242 1107 56-57 0.7630762070201307 1107 58-59 0.7442399460579132 1107 60-61 0.8230553551379707 1107 62-63 0.8649964631707938 1107 64-65 0.5757717940178892 1107 66-67 0.4839222475858804 1107 68-69 0.72023423469712 1107 70-71 0.9166973864986332 1107 72-73 0.5411478213490994 1107 74-75 0.70970836515475 1107 76-77 0.7061297124441275 1107 78-79 0.7565792344300881 1107 80-81 0.800374309069479 1107 82-83 1.1374538704585717 1107 84-85 1.033575188412172 1107 86-87 0.8702740713554817 1107 88-89 0.9698070274012416 1107 90-91 0.7774403293854988 1107 92-93 0.760282375766355 1107 94-95 1.1150083942034748 1107 96-97 0.793198083123837 1107 98-99 0.9068813382070573 1107 100-101 0.5516027749151533 1108 1 -0.0064668708713497836 1108 2 0.016127368070904424 1108 3 0.011707926887460474 1108 4 0.014411804397383321 1108 5 -0.05898459841010606 1108 6 0.07326649321372258 1108 7 0.07429119670093343 1108 8 0.14617091899289392 1108 9 -0.057850654958208736 1108 10-11 0.13803691781651395 1108 12-13 0.13792608858707212 1108 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-0.7820176187383474 2316 58-59 -0.7721842368130645 2316 60-61 -0.7797908685410029 2316 62-63 -0.6932897504438884 2316 64-65 -0.7259768773930517 2316 66-67 -0.8043858286715597 2316 68-69 -0.7267463065307922 2316 70-71 -0.45316038731991526 2316 72-73 -0.46117444692558607 2316 74-75 -0.6676330286822285 2316 76-77 -0.8404244562484315 2316 78-79 -0.5809248881911167 2316 80-81 -0.22065190910133836 2316 82-83 -0.8148580275112316 2316 84-85 -0.8663020679791664 2316 86-87 -0.9974943410358392 2316 88-89 -1.612407389702625 2316 90-91 -1.1722466914129122 2316 92-93 -1.2241111654037553 2316 94-95 -0.8544485078294493 2316 96-97 -0.8190154159530358 2316 98-99 -0.5534547956752789 2316 100-101 0.13731249478091812 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 8.0 4 34.0 5 106.0 6 251.0 7 422.0 8 587.0 9 939.0 10 1439.0 11 1925.0 12 2513.0 13 3438.0 14 4032.0 15 5344.0 16 6674.0 17 9202.0 18 8375.0 19 5814.0 20 5596.0 21 5843.0 22 6598.0 23 7157.0 24 8206.0 25 9038.0 26 10083.0 27 11296.0 28 12728.0 29 14736.0 30 17163.0 31 19885.0 32 23519.0 33 29313.0 34 44768.0 35 100397.0 36 54463.0 37 46241.0 38 22992.0 39 973.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.95281735576645 22.417199163452377 16.555772837770085 12.074210643011092 2 16.426010754829715 28.197371041625175 38.04879506074487 17.327823142800238 3 19.389962158932484 37.41465843457479 30.688906592312286 12.50647281418044 4 10.378012348137823 23.584146584345746 36.56144194383589 29.47639912368054 5 8.649870543716391 44.77773351921928 30.639912368054173 15.93248356901016 6 20.48317068313085 41.8832901812388 18.324039036048596 19.309500099581754 7 19.795857398924515 44.91495717984465 20.39075881298546 14.89842660824537 8 25.56184027086238 33.07209719179446 20.04023102967536 21.325831507667793 9 27.878709420434177 20.960963951404104 21.44732125074686 29.71300537741486 10-11 22.118502290380402 34.12238597888867 23.082254530969927 20.676857199761002 12-13 26.84564827723561 34.36944831706831 26.223859788886678 12.5610436168094 14-15 22.350053276770332 32.887202621017934 31.961680558460053 12.801063543751681 16-17 12.91278581692779 37.335525496846685 31.926849986506443 17.824838699719077 18-19 12.396845645136073 32.145321793787886 41.81969908354818 13.63813347752786 20-21 14.763792073292173 30.48317068313085 42.575682135032864 12.177355108544115 22-23 13.566321449910376 31.612527384983068 42.816172077275446 12.00497908783111 24-25 13.08504282015535 32.781816371240794 42.983867755427205 11.149273053176657 26-27 12.519381350286352 32.32415874732495 44.072468574080325 11.083991328308374 28-29 11.521111332403903 33.894941246763594 43.59221270663215 10.991734714200359 30-31 11.554705324093208 34.569410986774166 42.6976082758813 11.17827541325133 32-33 11.22266480780721 35.18611830312687 42.54879506074487 11.042421828321052 34-35 11.833921751211278 34.70274523082745 42.23831173034693 11.225021287614343 36-37 11.813192286237948 34.6157064307913 42.357259542593034 11.21384174037772 38-39 11.604153012866611 34.873714384747316 42.57475642284715 10.94737617953892 40-41 11.026410719656754 35.29503158171507 42.53751262372244 11.141045074905733 42-43 10.838497130781013 35.916526741200634 41.37073177533049 11.874244352687864 44-45 11.405149286281913 35.41573306724505 40.85152233966488 12.327595306808156 46-47 11.893793641878677 35.964791733884624 40.17861055013165 11.962804074105055 48-49 11.817935578185091 35.910989310830374 40.0084047504765 12.262670360508029 50-51 11.493228440549691 36.33150766779526 40.1458872734515 12.029376618203544 52-53 11.461385105327834 36.920507708639136 39.45825424866411 12.159852937368926 54-55 10.963652658832903 36.27544313881697 39.61551483768174 13.145389364668391 56-57 11.97948615813583 36.542919737104164 38.61023700458076 12.867357100179246 58-59 12.090551667270823 35.954572795462056 39.048912718740915 12.905962818526204 60-61 11.797922337116788 36.46058346255237 39.348018989970214 12.393475210360629 62-63 12.01522056070299 36.423885246293445 39.28167398283953 12.279220210164036 64-65 11.689789918978992 36.75352325868566 39.1791743345841 12.377512487751249 66-67 11.718492478825691 36.27481539388447 38.724313235374666 13.282378891915172 68-69 11.459633112489133 36.40105280244419 38.85589891025188 13.2834151748148 70-71 12.228838876717784 36.19468233419638 38.38070105556662 13.19577773351922 72-73 11.810894244174468 35.463951404102765 38.80870344552878 13.916450906193987 74-75 11.395140410276836 36.720673172674765 38.14897430790679 13.735212109141607 76-77 11.643642205359237 35.30839806624459 38.951900578230784 14.096059150165388 78-79 11.41471526944468 35.161099188902554 39.309197914747635 14.114987626905132 80-81 11.865497145907275 35.754731261178364 38.450683747405826 13.929087845508539 82-83 11.617230864829446 35.20212149396832 38.61768296690493 14.5629646742973 84-85 11.48088030272854 34.8308105954989 38.52877912766382 15.159529974108743 86-87 11.910802057800634 34.990684873610334 38.23248840967019 14.866024658918848 88-89 12.101691188229651 34.28674236366544 38.32090596513674 15.290660482968175 90-91 12.028606893077939 33.77100493904645 38.726641468401894 15.473746699473715 92-93 12.240489942242581 34.51354311890062 38.407886875124476 14.838080063732322 94-95 11.895538737303326 34.022107149970125 38.4484166500697 15.63393746265684 96-97 11.661919936267676 33.73242381995619 38.42750448117905 16.178151762597093 98-99 12.2079851661095 33.70856224985984 37.74167560424503 16.34177697978564 100-101 12.323080033101075 32.57697443828253 38.81977341099406 16.28017211762234 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 19424.0 1 12338.5 2 7539.5 3 9583.0 4 7686.5 5 6902.5 6 11391.5 7 21120.0 8 28563.0 9 26780.0 10 17551.0 11 9745.0 12 7775.5 13 7140.0 14 6329.5 15 5265.5 16 4254.5 17 3609.5 18 3201.5 19 2916.5 20 2725.5 21 2600.5 22 2561.0 23 2542.0 24 2574.0 25 2819.5 26 3105.0 27 3349.0 28 3675.5 29 4142.0 30 4834.0 31 5572.5 32 6833.5 33 8228.0 34 9159.5 35 10109.0 36 11517.0 37 12528.0 38 12174.0 39 11287.0 40 10379.5 41 9497.5 42 8428.5 43 8459.5 44 8778.5 45 8309.5 46 8225.0 47 10880.5 48 19574.0 49 19404.0 50 11428.0 51 7719.0 52 4873.0 53 3798.5 54 3533.0 55 3390.5 56 3201.5 57 2914.0 58 2777.5 59 2539.5 60 2087.5 61 1792.5 62 1539.5 63 1288.0 64 1030.0 65 846.5 66 711.5 67 605.5 68 480.0 69 326.5 70 276.0 71 247.5 72 197.0 73 183.5 74 177.5 75 126.5 76 80.0 77 66.5 78 56.5 79 40.0 80 26.5 81 22.5 82 19.5 83 9.0 84 3.0 85 2.5 86 1.5 87 1.0 88 1.0 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2929695279824736 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.958175662218682E-4 16-17 0.0016928898625771756 18-19 9.958175662218683E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0012945628360884285 28-29 0.0 30-31 0.002887870942043418 32-33 0.0 34-35 0.009460266879107747 36-37 0.027882891854212305 38-39 0.020513841864170487 40-41 0.013742282413861781 42-43 0.00936068512248556 44-45 0.00219079864568811 46-47 3.983270264887473E-4 48-49 5.974905397331208E-4 50-51 0.0 52-53 1.9916351324437365E-4 54-55 0.0 56-57 0.0 58-59 0.00438159729137622 60-61 0.009061939852619 62-63 0.0031866162119099785 64-65 0.0015933081059549892 66-67 0.003485361481776538 68-69 0.0022903804023102965 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.19966142202748455 78-79 4.979087831109341E-4 80-81 0.002788289185421231 82-83 1.9916351324437365E-4 84-85 0.0 86-87 0.0988846843258315 88-89 0.37044413463453496 90-91 0.3586934873531169 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0012945628360884285 100-101 2.987452698665604E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 502100.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.08086436504708 #Duplication Level Percentage of deduplicated Percentage of total 1 70.51493991919679 18.390905837420153 2 12.562620625856436 6.552880092250092 3 5.264901244634372 4.119395257700298 4 2.855544731652408 2.979002993382049 5 1.822932825315229 2.3771831881819274 6 1.2473604469182675 1.9519343178239907 7 0.84705878915739 1.546441777246443 8 0.6437517026812839 1.3431680673918944 9 0.5200070425325203 1.2206009830643936 >10 3.264267737795312 16.248112305735777 >50 0.29015664718862894 5.270390644023775 >100 0.13362477173160545 6.364895347577701 >500 0.01603497260779265 3.066644455972874 >1k 0.011453551862709037 6.446744225518142 >5k 0.0022907103725418074 4.465842062808734 >10k+ 0.00305428049672241 17.65585844390175 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28170 5.610436168094005 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 21583 4.298546106353316 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20758 4.134236207926708 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18147 3.6142202748456485 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7529 1.499502091216889 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7527 1.4991037641904004 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7369 1.4676359290977892 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4969 0.9896434973112926 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3831 0.7629954192391953 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3644 0.7257518422624976 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3618 0.7205735909181438 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2189 0.43596893049193386 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1950 0.3883688508265286 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1941 0.38657637920732923 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1569 0.31248755228042224 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1442 0.28719378609838675 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1392 0.27723561043616807 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1359 0.27066321449910374 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1245 0.24795857398924517 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1182 0.23541127265484965 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1029 0.20493925512846045 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1012 0.2015534754033061 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 991 0.19737104162517427 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 989 0.1969727145986855 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 912 0.18163712407886876 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 870 0.17327225652260506 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 849 0.1690898227444732 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 841 0.16749651463851822 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 835 0.166301533559052 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 831 0.16550487950607448 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 793 0.15793666600278827 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 792 0.15773750248954393 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 791 0.15753833897629954 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 682 0.1358295160326628 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 675 0.13443537143995218 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 644 0.12826130252937662 No Hit GTACATGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 621 0.12368054172475602 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 611 0.1216889065923123 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 581 0.11571400119498108 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 541 0.10774746066520614 No Hit ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT 528 0.10515833499302926 TruSeq Adapter, Index 3 (95% over 21bp) AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 517 0.10296753634734115 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 505 0.1005775741884087 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 9.958175662218681E-5 0.0 16-17 0.0 0.0 0.0 2.987452698665604E-4 0.0 18-19 0.0 0.0 0.0 3.9832702648874725E-4 0.0 20-21 0.0 0.0 0.0 7.966540529774945E-4 0.0 22-23 0.0 0.0 0.0 0.0029874526986656044 0.0 24-25 0.0 0.0 0.0 0.005476996614220275 0.0 26-27 0.0 0.0 0.0 0.012746464847639912 0.0 28-29 0.0 0.0 0.0 0.02519418442541326 0.0 30-31 0.0 0.0 0.0 0.05785700059749054 0.0 32-33 0.0 0.0 0.0 0.0952001593308106 0.0 34-35 0.0 0.0 0.0 0.14021111332403902 0.0 36-37 0.0 0.0 0.0 0.20284803823939454 0.0 38-39 0.0 0.0 0.0 0.2833100975901215 0.0 40-41 0.0 0.0 0.0 0.377912766381199 0.0 42-43 0.0 0.0 0.0 0.46395140410276836 0.0 44-45 0.0 0.0 0.0 0.5584544911372237 0.0 46-47 0.0 0.0 0.0 0.6698864767974506 0.0 48-49 0.0 0.0 0.0 0.794761999601673 0.0 50-51 0.0 0.0 0.0 0.934973112925712 0.0 52-53 0.0 0.0 0.0 1.0568611830312689 0.0 54-55 0.0 0.0 0.0 1.189205337582155 0.0 56-57 0.0 0.0 0.0 1.342262497510456 0.0 58-59 0.0 0.0 0.0 1.5070703047201754 0.0 60-61 0.0 0.0 0.0 1.7176857199761004 0.0 62-63 0.0 0.0 0.0 1.916849233220474 0.0 64-65 0.0 0.0 0.0 2.0984863572993424 0.0 66-67 0.0 0.0 0.0 2.3014339772953596 0.0 68-69 0.0 0.0 0.0 2.5302728540131447 0.0 70-71 0.0 0.0 0.0 2.7880900219079865 0.0 72-73 0.0 0.0 0.0 3.0532762397928703 0.0 74-75 0.0 0.0 0.0 3.3221469826727743 0.0 76-77 0.0 0.0 0.0 3.590121489743079 0.0 78-79 0.0 0.0 0.0 3.922724556861183 0.0 80-81 0.0 0.0 0.0 4.267576180043816 0.0 82-83 0.0 0.0 0.0 4.6132244572794265 0.0 84-85 0.0 0.0 0.0 4.994423421629158 0.0 86-87 0.0 0.0 0.0 5.40161322445728 0.0 88-89 0.0 0.0 0.0 5.785002987452699 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATGGT 25 5.2374256E-5 76.1022 1 TCACGCA 35 2.799519E-4 54.23422 4 TATCACG 40 5.41052E-4 47.454937 2 GGATGTC 55 4.9871538E-5 43.140854 7 GGATGTA 95 1.9406798E-8 39.96205 8 TAGGACA 110 1.6316335E-9 38.826767 4 CATTGGG 50 0.0016224965 37.96395 2 CACGCAG 50 0.0016224965 37.96395 5 ATCACGC 50 0.0016224965 37.96395 3 GTTCGCA 50 0.0016224965 37.96395 9 GATGTAA 110 7.057861E-8 34.512684 9 GGGTATG 70 2.0631612E-4 33.896385 9 GGGAATG 170 3.6379788E-12 33.497604 7 CATGAGA 330 0.0 33.074654 4 GGTAAGG 60 0.003922951 31.709251 1 CGTAAAC 30 0.003959449 31.652386 32-33 GAGCTTA 75 3.0931682E-4 31.636625 9 AGTGCTA 60 0.003967241 31.636623 8 ATGAGAG 315 0.0 30.13012 5 GATGTCC 80 4.5151016E-4 29.659336 8 >>END_MODULE