##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939080_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 500652 Sequences flagged as poor quality 0 Sequence length 101 %GC 27 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.516120978244366 33.0 31.0 34.0 31.0 34.0 2 32.51526609301471 34.0 31.0 34.0 31.0 34.0 3 32.49075805150084 34.0 31.0 34.0 31.0 34.0 4 35.99809049000104 37.0 35.0 37.0 35.0 37.0 5 36.08754983501514 37.0 37.0 37.0 35.0 37.0 6 36.262246031175344 37.0 37.0 37.0 35.0 37.0 7 36.240246718279366 37.0 37.0 37.0 35.0 37.0 8 36.246518539824066 37.0 37.0 37.0 35.0 37.0 9 37.92373345157914 39.0 39.0 39.0 37.0 39.0 10-11 37.91179402059714 39.0 38.0 39.0 37.0 39.0 12-13 37.79735924354641 39.0 38.0 39.0 35.0 39.0 14-15 38.93457631248852 40.5 38.5 41.0 36.0 41.0 16-17 38.94407692369151 40.0 38.5 41.0 36.5 41.0 18-19 38.98898736048193 40.5 38.5 41.0 35.5 41.0 20-21 39.01670022290933 40.5 39.0 41.0 35.0 41.0 22-23 38.89113795610524 41.0 38.5 41.0 35.0 41.0 24-25 38.74321984132691 41.0 38.0 41.0 35.0 41.0 26-27 38.49621493572381 40.0 37.0 41.0 35.0 41.0 28-29 38.21705396163403 40.0 36.0 41.0 35.0 41.0 30-31 37.62432488035601 39.0 35.0 41.0 35.0 41.0 32-33 36.99914411607264 38.0 35.0 41.0 35.0 41.0 34-35 36.57770367440857 37.5 35.0 41.0 34.5 41.0 36-37 36.25538298059331 36.5 35.0 40.5 33.5 41.0 38-39 35.98701293513258 35.0 35.0 40.0 33.0 41.0 40-41 35.72321992122272 35.0 35.0 40.0 33.0 41.0 42-43 35.40339896774606 35.0 35.0 40.0 33.0 41.0 44-45 34.986934038014425 35.0 35.0 40.0 32.0 41.0 46-47 34.357537770746944 35.0 35.0 40.0 27.0 41.0 48-49 34.0330409146473 35.0 35.0 40.0 23.5 41.0 50-51 32.92315220951879 35.0 33.5 39.0 21.5 40.0 52-53 33.22810455166463 35.0 34.0 39.0 23.0 40.5 54-55 33.630727531299186 35.0 35.0 40.0 23.0 41.0 56-57 33.51484863737686 35.0 35.0 40.0 22.0 41.0 58-59 33.33947332678188 35.0 35.0 40.0 20.0 41.0 60-61 33.06334439890383 35.0 35.0 39.5 15.5 41.0 62-63 32.71477393478904 35.0 35.0 39.0 4.5 41.0 64-65 32.3803859766864 35.0 35.0 39.0 2.0 41.0 66-67 32.01737634125101 35.0 34.0 38.0 2.0 40.5 68-69 31.645705000679115 35.0 34.0 37.0 2.0 40.0 70-71 31.234155661018033 35.0 33.0 37.0 2.0 39.5 72-73 30.805477657135096 35.0 33.0 36.0 2.0 39.0 74-75 30.406800532106132 35.0 33.0 35.5 2.0 39.0 76-77 29.982352012975078 35.0 33.0 35.0 2.0 37.5 78-79 29.570584957215793 35.0 32.0 35.0 2.0 37.0 80-81 29.12631628356623 35.0 31.0 35.0 2.0 36.5 82-83 28.789036096929603 35.0 31.0 35.0 2.0 36.0 84-85 28.410088244928613 35.0 31.0 35.0 2.0 36.0 86-87 27.992732476850186 35.0 29.5 35.0 2.0 35.0 88-89 27.51656639741777 35.0 29.0 35.0 2.0 35.0 90-91 27.056225681711048 35.0 27.0 35.0 2.0 35.0 92-93 26.617010418414388 34.0 25.5 35.0 2.0 35.0 94-95 26.12690451651047 34.0 22.0 35.0 2.0 35.0 96-97 25.55612681063893 34.0 10.0 35.0 2.0 35.0 98-99 25.00480273723065 34.0 2.0 35.0 2.0 35.0 100-101 22.78938264503088 31.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.056255502470371255 1101 2 0.04450666678941673 1101 3 0.04013660036866895 1101 4 0.07680854796457481 1101 5 0.00422611899858083 1101 6 0.04582118259232715 1101 7 -0.022544338268815522 1101 8 0.02032336563070203 1101 9 0.021950941309555105 1101 10-11 0.08385661619386298 1101 12-13 0.18267927736805945 1101 14-15 0.26096062646384866 1101 16-17 0.34511279788264915 1101 18-19 0.2608184440551611 1101 20-21 0.2827291109015633 1101 22-23 0.32682741761441747 1101 24-25 0.2572830616661079 1101 26-27 0.08826251240081007 1101 28-29 0.2188672288483957 1101 30-31 0.34115643035072907 1101 32-33 0.3751623171242713 1101 34-35 0.453057614729218 1101 36-37 0.4088564911617212 1101 38-39 0.4014851044294403 1101 40-41 0.28092133204239644 1101 42-43 0.15429482134226902 1101 44-45 0.2204304828203476 1101 46-47 0.3120517505369307 1101 48-49 0.17715260419544165 1101 50-51 0.16501125543902617 1101 52-53 0.23686114707777506 1101 54-55 0.3481372146749351 1101 56-57 0.19985801512137868 1101 58-59 0.003410880330953603 1101 60-61 0.0011380617216047995 1101 62-63 0.053236599042527644 1101 64-65 0.0015033190279396536 1101 66-67 0.07644264985982119 1101 68-69 -0.017240644627516843 1101 70-71 -0.08903656152025619 1101 72-73 -0.024781436790682676 1101 74-75 0.044657892010857125 1101 76-77 0.06960912947198139 1101 78-79 0.022365733180688352 1101 80-81 0.11507630758012155 1101 82-83 0.004712332321318513 1101 84-85 -0.3211361547878582 1101 86-87 -0.2336210864088919 1101 88-89 -0.0984443362868781 1101 90-91 -0.18382411086105677 1101 92-93 -0.08528939020839843 1101 94-95 -0.27774060962214975 1101 96-97 -0.7157734107774338 1101 98-99 -0.5999967161630799 1101 100-101 -0.5573440811042509 1102 1 0.11465545171539304 1102 2 0.10122535731092341 1102 3 0.15627033974184457 1102 4 -0.0735848031330022 1102 5 0.1254988001304227 1102 6 0.10644166222688511 1102 7 0.11385967137462671 1102 8 0.15031955900717264 1102 9 0.22148145774147565 1102 10-11 0.08702880246463707 1102 12-13 0.05832957555357865 1102 14-15 0.12076585422682484 1102 16-17 0.18918398174256623 1102 18-19 0.24070678309843174 1102 20-21 0.20745313069600257 1102 22-23 0.22945716003289363 1102 24-25 0.34041020289183876 1102 26-27 0.2876502804505563 1102 28-29 0.02790161534757374 1102 30-31 0.23660117086588883 1102 32-33 0.22218363421600884 1102 34-35 0.1261479585942098 1102 36-37 -0.035630249099668276 1102 38-39 -0.0037141088683583234 1102 40-41 0.08032813320976118 1102 42-43 0.23017351693927424 1102 44-45 -0.12663841706545043 1102 46-47 0.012375313536544752 1102 48-49 0.08265317518896609 1102 50-51 0.19562285295203452 1102 52-53 0.31183576958760995 1102 54-55 0.17330216836101187 1102 56-57 0.16911953015753767 1102 58-59 0.24094418828679665 1102 60-61 0.06001383890723844 1102 62-63 0.04719358419669106 1102 64-65 -0.07334441603106256 1102 66-67 -0.16307327451525566 1102 68-69 -0.1487119046199048 1102 70-71 -0.05196956494621574 1102 72-73 -0.18266114748729478 1102 74-75 -0.37748079597290385 1102 76-77 -0.20692620868307543 1102 78-79 -0.051958591143634436 1102 80-81 -0.13194183732534626 1102 82-83 -0.3211187804816227 1102 84-85 -0.25172871918323736 1102 86-87 -0.1610890473275539 1102 88-89 -0.022803554660182357 1102 90-91 0.3109100599294514 1102 92-93 0.1892950533901292 1102 94-95 0.04127030269862075 1102 96-97 0.17535715640947203 1102 98-99 0.3225860778985883 1102 100-101 0.31362724233186157 1103 1 0.20797567725271193 1103 2 0.212620817436175 1103 3 0.14854610280283254 1103 4 0.09830937742168544 1103 5 0.17550088073296166 1103 6 0.09116483805861009 1103 7 0.12127701943862945 1103 8 0.09872061595326898 1103 9 -0.07451546296675815 1103 10-11 0.14106205049326803 1103 12-13 0.20327475167658093 1103 14-15 0.2225047178017192 1103 16-17 0.31295642624248643 1103 18-19 0.4591752515668972 1103 20-21 0.29428187630073666 1103 22-23 0.33296973727526336 1103 24-25 0.5211859555498037 1103 26-27 0.4925090075379188 1103 28-29 0.609357204886301 1103 30-31 0.5405459932864645 1103 32-33 0.6082316337166631 1103 34-35 0.6998455014026135 1103 36-37 0.45344658755222866 1103 38-39 0.3985521874510596 1103 40-41 0.4642747241424914 1103 42-43 0.6194386481212675 1103 44-45 0.2546632078851374 1103 46-47 0.33246561168689226 1103 48-49 0.30806045313666175 1103 50-51 0.3820552437724061 1103 52-53 0.5932918792769826 1103 54-55 0.42404338301678024 1103 56-57 0.35946345375781874 1103 58-59 0.24978674909240084 1103 60-61 0.05816730415077842 1103 62-63 -0.017879861073062386 1103 64-65 0.16180268256869113 1103 66-67 0.2300331691108184 1103 68-69 0.3887623479236346 1103 70-71 0.4357522792998765 1103 72-73 0.5415913960493164 1103 74-75 0.4641463392454561 1103 76-77 0.5017400210570564 1103 78-79 0.4423041473454319 1103 80-81 0.22705572836968102 1103 82-83 0.1897029534925352 1103 84-85 0.24342867839910198 1103 86-87 0.1879762419802269 1103 88-89 -0.034858045640770996 1103 90-91 0.2571732977631278 1103 92-93 0.46409583569668555 1103 94-95 0.41381519169847536 1103 96-97 0.40420783156499596 1103 98-99 0.413973943824562 1103 100-101 -0.37894032523570687 1104 1 0.1280570822876541 1104 2 0.2988592763271285 1104 3 0.26162029012419197 1104 4 0.2525150548243076 1104 5 0.28623567091048585 1104 6 0.2218318904680885 1104 7 0.3141301362239233 1104 8 0.3316477554541635 1104 9 0.31831371116120266 1104 10-11 0.29613315519750216 1104 12-13 0.3674864646240721 1104 14-15 0.4159738592080515 1104 16-17 0.2591278456929089 1104 18-19 0.2507519254329864 1104 20-21 0.34600799225302836 1104 22-23 0.41380862813166175 1104 24-25 0.5095164041254634 1104 26-27 0.5448910525401374 1104 28-29 0.4206691128666904 1104 30-31 0.5660623815366037 1104 32-33 0.4728184482957474 1104 34-35 0.4867782973562953 1104 36-37 0.5677204017671826 1104 38-39 0.6481718483631695 1104 40-41 0.7005463535899636 1104 42-43 0.7541310597953057 1104 44-45 0.7301319602232894 1104 46-47 0.8057421144627455 1104 48-49 0.6766202524173863 1104 50-51 0.5277887121157292 1104 52-53 0.3664127547801499 1104 54-55 0.29447869045546327 1104 56-57 0.21899986405515648 1104 58-59 0.32296897765499466 1104 60-61 0.345835838012178 1104 62-63 0.2070913329173365 1104 64-65 0.334164875117537 1104 66-67 0.2353978207700962 1104 68-69 0.18364610133421522 1104 70-71 0.26012789968904215 1104 72-73 0.17696467704134022 1104 74-75 0.09452657857226754 1104 76-77 0.07891768056254378 1104 78-79 0.09004031921380928 1104 80-81 0.025141812198906877 1104 82-83 -0.08099123392858942 1104 84-85 -0.031752263245657275 1104 86-87 0.14123568561273103 1104 88-89 0.22482501343275274 1104 90-91 0.10643182359376269 1104 92-93 -0.16035859593954527 1104 94-95 -0.11824801691689046 1104 96-97 -0.04862762751051619 1104 98-99 0.15326583973385866 1104 100-101 0.13293872444031862 1105 1 -0.14264596735073098 1105 2 -0.024841886403379476 1105 3 0.061631583338382256 1105 4 -0.25489846974078745 1105 5 -0.1590651756100243 1105 6 -0.06780611496206745 1105 7 -0.20366373438407237 1105 8 -0.18160059644239368 1105 9 -0.20008716970950502 1105 10-11 -0.07536888934482278 1105 12-13 -0.04474408634361993 1105 14-15 0.086047312155344 1105 16-17 -0.11877839239628685 1105 18-19 0.09751947814641682 1105 20-21 -0.11002611325383072 1105 22-23 -0.013425428437933817 1105 24-25 -0.052269241258194654 1105 26-27 -0.08609846829819645 1105 28-29 -0.29662879741813697 1105 30-31 0.012671358700778512 1105 32-33 0.1376358511388105 1105 34-35 0.028174985621241433 1105 36-37 0.1420179873720926 1105 38-39 0.2491637690095132 1105 40-41 0.39777057565220275 1105 42-43 0.028692624281909218 1105 44-45 -0.19082024595316227 1105 46-47 -0.18617617913259465 1105 48-49 -0.3836808058508936 1105 50-51 -0.5020159623338429 1105 52-53 -0.33097615998902796 1105 54-55 -0.12676996259347106 1105 56-57 -0.11772613904106777 1105 58-59 -0.2746890920523697 1105 60-61 -0.1778563842570975 1105 62-63 -0.21946277804790526 1105 64-65 -0.24779092135991476 1105 66-67 -0.21015712306969192 1105 68-69 -0.6828563431761054 1105 70-71 -0.6638240665066029 1105 72-73 -0.4888644095729049 1105 74-75 -0.43835784949701306 1105 76-77 -0.3817701702917411 1105 78-79 -0.4852595391504657 1105 80-81 -0.4393669094563002 1105 82-83 -0.42624596649115887 1105 84-85 -0.744706991573274 1105 86-87 -0.7794647759974005 1105 88-89 -0.7429869733730108 1105 90-91 -0.7238452836494211 1105 92-93 -0.44238074005036765 1105 94-95 -0.43222525167928794 1105 96-97 -0.39740678400152873 1105 98-99 -0.4524315868249573 1105 100-101 -0.18612986771936946 1106 1 0.07029927341127973 1106 2 0.027081799436345477 1106 3 0.028654111800236137 1106 4 0.22984389671652394 1106 5 0.1187811280114488 1106 6 0.05920299188626643 1106 7 0.07804837200584558 1106 8 0.12819765402193184 1106 9 -0.08835223712475226 1106 10-11 0.26357411133098907 1106 12-13 0.08308969358918006 1106 14-15 -0.034607794910378686 1106 16-17 0.05309860135132993 1106 18-19 0.039435555254534904 1106 20-21 0.08861140393670297 1106 22-23 0.07476260647717226 1106 24-25 0.19079665224791853 1106 26-27 0.0778366164611839 1106 28-29 -0.06115273184126835 1106 30-31 0.07256294609013736 1106 32-33 0.04022754580655885 1106 34-35 0.013352136741993093 1106 36-37 0.07488156423210057 1106 38-39 0.08185437718819344 1106 40-41 0.08452240340186279 1106 42-43 -0.06363059085615674 1106 44-45 0.010265297929372252 1106 46-47 -0.07147100566768927 1106 48-49 -0.008497085700994944 1106 50-51 -0.07490146746383175 1106 52-53 -0.19335027328823884 1106 54-55 -0.20753121382811912 1106 56-57 -0.1720600442107525 1106 58-59 -0.1367111242925958 1106 60-61 -0.41867037676178853 1106 62-63 -0.5312908112616768 1106 64-65 -0.49947689341082935 1106 66-67 -0.5992114191675029 1106 68-69 -0.7656213845987985 1106 70-71 -0.8055922223107395 1106 72-73 -0.4874773519781179 1106 74-75 -0.4213020032973631 1106 76-77 -0.23719481785623842 1106 78-79 0.007542884301443564 1106 80-81 0.1485588368846571 1106 82-83 0.062206247366184186 1106 84-85 -0.012496536892484755 1106 86-87 -0.03891779798710715 1106 88-89 -0.17509440299224366 1106 90-91 0.1681485348468854 1106 92-93 0.030586996092051066 1106 94-95 0.24008652673176556 1106 96-97 0.38674153427974645 1106 98-99 0.1575878367072896 1106 100-101 0.21433516283370935 1107 1 0.03531236655835812 1107 2 0.13489962354332619 1107 3 0.22565208935615289 1107 4 0.10295272805569056 1107 5 0.11918011357917635 1107 6 0.03548857383194104 1107 7 0.16438059398857519 1107 8 -0.017289910927878793 1107 9 0.020988152597013254 1107 10-11 0.025622787570249272 1107 12-13 0.09483225159113573 1107 14-15 0.1984066557048365 1107 16-17 0.12783272562386827 1107 18-19 0.15367842281425936 1107 20-21 0.09716895645834 1107 22-23 0.07025911420694086 1107 24-25 0.029916040653482412 1107 26-27 0.14438465753377017 1107 28-29 0.21580516371623304 1107 30-31 0.17180487351145501 1107 32-33 0.15595170461198649 1107 34-35 0.11520810753807353 1107 36-37 0.04973630138315599 1107 38-39 0.06754134320104299 1107 40-41 0.008020360320593056 1107 42-43 0.003476947219446913 1107 44-45 -0.2629068207757115 1107 46-47 -0.21862101048303373 1107 48-49 -0.25742739163578676 1107 50-51 -0.3068817658316618 1107 52-53 -0.34341012112339087 1107 54-55 -0.3035491544205442 1107 56-57 -0.28545449418392366 1107 58-59 -0.4802812654967994 1107 60-61 -0.4508093521485037 1107 62-63 -0.49064472980440854 1107 64-65 -0.5018937370222432 1107 66-67 -0.3803013376735507 1107 68-69 -0.39656202747002567 1107 70-71 -0.20290617809213174 1107 72-73 -0.080881249195766 1107 74-75 -0.06301067045626496 1107 76-77 -0.15440301953197988 1107 78-79 -0.06290558464876383 1107 80-81 0.0019362267852791604 1107 82-83 -0.05894090493165294 1107 84-85 -0.18689909040012154 1107 86-87 -0.14998253912802184 1107 88-89 -0.0017833272446061699 1107 90-91 0.0932034526879626 1107 92-93 0.203003793843056 1107 94-95 -0.11182333004617462 1107 96-97 -0.191388553359495 1107 98-99 -0.1415802554537926 1107 100-101 -0.4550502618107579 1108 1 -0.17605192232531408 1108 2 -0.08112365837142477 1108 3 -0.03761799303537572 1108 4 -0.11026036758634206 1108 5 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58-59 0.08893396911239648 2316 60-61 -0.3662445089928923 2316 62-63 -0.27534350513144545 2316 64-65 -0.2527010114324284 2316 66-67 -0.2665256074451996 2316 68-69 -0.31409557186924175 2316 70-71 -0.2642695514999325 2316 72-73 -0.7068756605077766 2316 74-75 -0.2549123319865281 2316 76-77 -0.14670367271427054 2316 78-79 -0.1376674343151656 2316 80-81 0.004134537556996776 2316 82-83 0.15369745366297138 2316 84-85 -0.016631681847393054 2316 86-87 0.14546937676817961 2316 88-89 0.08618416440216592 2316 90-91 0.00510798920200628 2316 92-93 0.17825789683058701 2316 94-95 0.32709904188557104 2316 96-97 0.15640910387933005 2316 98-99 -0.027448658330175846 2316 100-101 0.11940129997158877 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2301.0 3 323.0 4 278.0 5 273.0 6 257.0 7 322.0 8 394.0 9 464.0 10 546.0 11 903.0 12 1607.0 13 3471.0 14 2994.0 15 3530.0 16 5051.0 17 8587.0 18 6688.0 19 3883.0 20 3855.0 21 3843.0 22 3931.0 23 4330.0 24 4725.0 25 5229.0 26 5821.0 27 6434.0 28 7459.0 29 8374.0 30 9894.0 31 11936.0 32 14359.0 33 19050.0 34 31030.0 35 101742.0 36 75042.0 37 64020.0 38 66219.0 39 11485.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.23189253975444 21.709911852775296 15.570070830443864 12.488124777026403 2 15.457822581128877 27.142660063459566 39.3587264900702 18.04079086534136 3 19.423268014627233 38.11217543080956 29.44839982165814 13.016156732905074 4 10.022595932732107 22.234597772400967 37.42026435241657 30.32254194245036 5 8.752712038953378 44.94076007078797 30.127678294689904 16.178849595568753 6 20.81801920636348 41.08842721667616 19.357056489892177 18.736497087068184 7 20.67800743125886 44.60893949660117 20.413776010905043 14.299277061234934 8 25.815065317151948 32.21126940726883 20.412009721622614 21.561655553956605 9 29.34424591277931 20.696126633888955 21.472198904744776 28.487428548586962 10-11 21.981220882474624 34.049941739966386 23.174002634192945 20.79483474336605 12-13 26.613215117667693 34.30196360990829 26.27933447724845 12.805486795175568 14-15 22.811423249418404 32.515369302965134 31.668258891811302 13.004948555805159 16-17 13.417203136273606 37.68245129801994 31.074831264402736 17.825514301303716 18-19 12.842491765006875 33.154841856151464 40.54145079791487 13.461215580926797 20-21 15.544538030117087 31.468737632766626 40.50356376195749 12.4831605751588 22-23 13.940249221432882 32.8874145974838 40.70466820447831 12.467667976605009 24-25 13.32526365556403 33.895745227411986 40.9807357267158 11.79825539030819 26-27 12.696975239968694 33.76434239631843 41.97010724249158 11.568575121221299 28-29 11.73425634034251 35.36168987844054 41.58698967913979 11.317064102077165 30-31 11.623232873426515 36.21333946320329 40.56987801891931 11.593549644450887 32-33 11.2981035904355 36.78095095320191 40.385578292481824 11.53536716388077 34-35 11.828450306925456 36.638825669546264 39.844113121664286 11.68861090186399 36-37 11.88860311346912 36.44914396156984 40.200740786084225 11.461512138876818 38-39 11.77819738217689 36.788982909704565 40.169919715543 11.262899992575544 40-41 11.013557431510696 37.37993307547866 40.41771654175159 11.188792951259048 42-43 10.877039846293538 37.93630010885869 39.03130816038848 12.155351884459293 44-45 11.300010435564403 37.49026682935444 38.74955849535216 12.460164239728996 46-47 11.791962032619438 37.906576564373474 38.22173391729252 12.079727485714573 48-49 11.721050830688462 37.87471791110224 38.02402810403148 12.380203154177826 50-51 11.436618813823442 38.18092970378197 38.31207599460966 12.070375487784931 52-53 11.317312815511185 38.69297218434332 37.67771727187619 12.3119977282693 54-55 10.867942399383539 37.98963734728933 38.05104268674447 13.09137756658265 56-57 11.946715350432838 38.218194220140234 36.89661052652697 12.93847990289996 58-59 11.940650307019215 37.6095028515375 37.50378633629086 12.946060505152426 60-61 11.670631733972556 37.79847031613266 38.10123904593763 12.429658903957147 62-63 11.88414026688551 37.84272395378211 37.961709824962355 12.311425954370021 64-65 11.599956247786047 38.07787291988829 38.028400806405486 12.293770025920177 66-67 11.629582721567832 37.810971736182964 37.37742527595935 13.182020266289857 68-69 11.278311711123937 38.01470477528795 37.6916012145241 13.015382299064013 70-71 12.041745037306766 37.800978386765884 37.08555564700191 13.07172092892544 72-73 11.680513977939974 37.12430867980046 37.51509087642994 13.680086465829639 74-75 11.372602544506789 38.19610385569955 37.09957939465347 13.331714205140196 76-77 11.75705097976261 36.8722847080779 37.56158006160514 13.809084250554346 78-79 11.513850505820573 36.66002939125986 37.97461116767563 13.851508935243933 80-81 12.014618501453123 37.2192945760464 37.063699628518314 13.702387293982158 82-83 11.817060849621202 36.321251165091304 37.42719747005878 14.43449051522871 84-85 11.78217424455235 35.983085196035155 37.15819254384205 15.076548015570449 86-87 12.254011314852473 36.019114255128954 37.30088899545821 14.425985434560362 88-89 12.461145770833793 35.18622939898101 37.198722952540145 15.153901877645048 90-91 12.343173913130741 34.66069769168859 37.73098668721889 15.265141707961776 92-93 12.78737881616186 35.181148511671786 37.520423114750805 14.511049557415548 94-95 12.473869763791697 34.64876847933315 37.57886740040403 15.298494356471116 96-97 12.266664525782435 34.09234369621655 37.70187451140171 15.939117266599295 98-99 12.968499266899695 34.01495103130805 37.04289113485533 15.973658566936924 100-101 13.354342938154105 32.679467074417545 38.2903559492599 15.675834038168452 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 20131.0 1 12502.5 2 7208.5 3 9242.0 4 7355.5 5 6806.0 6 11354.0 7 20500.5 8 28051.5 9 26291.5 10 17018.0 11 9955.0 12 8309.0 13 7722.5 14 6781.5 15 5586.5 16 4476.5 17 3685.0 18 3177.5 19 2895.5 20 2728.5 21 2665.0 22 2592.0 23 2569.0 24 2640.5 25 2826.0 26 3171.5 27 3443.0 28 3670.0 29 4108.0 30 4797.0 31 5493.5 32 6705.5 33 8080.0 34 9124.5 35 9999.5 36 11606.0 37 12768.0 38 12022.5 39 11037.5 40 10009.5 41 9037.0 42 7960.0 43 8026.5 44 8423.0 45 7982.5 46 7894.5 47 10261.5 48 20541.5 49 20116.5 50 10437.5 51 7238.0 52 4738.5 53 3943.5 54 3758.0 55 3552.0 56 3299.0 57 3080.5 58 2934.5 59 2606.5 60 2212.5 61 1950.5 62 1668.0 63 1396.0 64 1115.5 65 948.5 66 846.0 67 698.5 68 555.0 69 410.5 70 369.0 71 336.0 72 251.0 73 222.0 74 199.0 75 141.0 76 89.5 77 67.5 78 67.5 79 63.0 80 50.0 81 32.5 82 23.5 83 18.0 84 11.5 85 11.5 86 10.0 87 6.0 88 3.5 89 0.5 90 1.0 91 1.0 92 0.5 93 1.5 94 1.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34235357094348967 2 0.09807211396339174 3 0.09767263488411113 4 0.11225362127785367 5 0.11325231897605521 6 0.059322643273171785 7 0.06651326670022291 8 0.06471561084346013 9 0.06231873636777642 10-11 0.06171951774885549 12-13 0.06461574107363997 14-15 0.06072082005065395 16-17 0.06251847590741673 18-19 0.06950935979482754 20-21 0.06751196439842445 22-23 0.07470258782547558 24-25 0.08498917411695149 26-27 0.08598787181515305 28-29 0.08049503447504454 30-31 0.0738037598970942 32-33 0.07280506219889264 34-35 0.301207225777586 36-37 0.47887554628764095 38-39 0.45920120163307043 40-41 0.43543219641587366 42-43 0.4593010714028906 44-45 0.4705863553925681 46-47 0.4652932575920999 48-49 0.47148518332094946 50-51 0.47008700654346736 52-53 0.47008700654346736 54-55 0.46419469012407816 56-57 0.43882776858975897 58-59 0.4292402706870241 60-61 0.3837995254188538 62-63 0.4541078433722426 64-65 0.4776771090497991 66-67 0.4864656487939727 68-69 0.502245072425557 70-71 0.496352756006168 72-73 0.483469555699368 74-75 0.48766408603181455 76-77 0.462796513346596 78-79 0.44032581513706126 80-81 0.447915917643393 82-83 0.4595008109425309 84-85 0.45380823406278215 86-87 0.45550602014972474 88-89 0.46239703426731543 90-91 0.4811725509935045 92-93 0.4877639558016346 94-95 0.48466799293720986 96-97 0.4824708580011665 98-99 0.48446825339756955 100-101 0.46109872725965345 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 500652.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.722881001687398 #Duplication Level Percentage of deduplicated Percentage of total 1 70.38560859418514 18.809062426937558 2 12.734804774225978 6.806213451227228 3 5.279166512075542 4.232236154708636 4 2.8793034664103714 3.077731356025216 5 1.7873141942632504 2.388109226296182 6 1.1824731530261419 1.8959453613604613 7 0.9075369126464805 1.6976400644903467 8 0.6050849987340202 1.2935691533660316 9 0.5407055087008992 1.3004288069373882 >10 3.235098519491851 16.556392161809203 >50 0.29314720446884845 5.325343023466438 >100 0.13834753272126774 6.506798590665987 >500 0.01570431452511688 3.119306486538863 >1k 0.010469543016744586 6.012793488819355 >5k 0.002243473503588126 4.263791607200536 >10k+ 0.0029912980047841677 16.714638640150586 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27627 5.518204261642818 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 21710 4.336345405591109 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18308 3.6568314917347777 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15995 3.1948339365467433 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7256 1.4493100996300823 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7206 1.439323122648067 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6874 1.3730095954874844 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4510 0.9008253237777937 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3455 0.6901001094572677 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3073 0.6137996053146697 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2898 0.5788451858776156 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2446 0.4885629139601959 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2080 0.41545824245184276 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1918 0.3831004370301127 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1787 0.35693455733723223 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1692 0.3379593010714029 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1536 0.30679993288751467 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1254 0.25047338270894753 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1184 0.23649161493412588 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1150 0.2297004705863554 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1105 0.22071219130254147 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 930 0.18575777186548742 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 926 0.18495881370692616 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 910 0.1817629810726812 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 907 0.18116376245376029 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 899 0.1795658461366378 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 855 0.17077730639246422 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 846 0.16897965053570144 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 833 0.16638303652037745 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 828 0.1653843388221759 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 736 0.14700830117526745 No Hit GTACATGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 733 0.14640908255634652 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 718 0.14341298946174189 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 699 0.13961793820857601 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 688 0.13742080327253262 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 671 0.13402523109864736 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 657 0.13122887754368304 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 618 0.12343903549771099 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 569 0.11365179805533585 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 539 0.10765961186612658 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 527 0.10526273739044287 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 520 0.10386456061296069 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.996093094604636E-4 0.0 16-17 0.0 0.0 0.0 3.994790792806181E-4 0.0 18-19 0.0 0.0 0.0 3.994790792806181E-4 0.0 20-21 0.0 0.0 0.0 0.0019973953964030904 0.0 22-23 0.0 0.0 0.0 0.004893618721187571 0.0 24-25 0.0 0.0 0.0 0.009387758363094524 0.0 26-27 0.0 0.0 0.0 0.01767694925816735 0.0 28-29 0.0 0.0 0.0 0.033056893810471145 0.0 30-31 0.0 0.0 0.0 0.07130701565159032 0.0 32-33 0.0 0.0 0.0 0.11894489585580403 0.0 34-35 0.0 0.0 0.0 0.1683804319167805 0.0 36-37 0.0 0.0 0.0 0.22980034035617555 0.0 38-39 0.0 0.0 0.0 0.31468964470330685 0.0 40-41 0.0 0.0 0.0 0.4042728282319855 0.0 42-43 0.0 0.0 0.0 0.49135926751516024 0.0 44-45 0.0 0.0 0.0 0.596522135135783 0.0 46-47 0.0 0.0 0.0 0.7205603892524148 0.0 48-49 0.0 0.0 0.0 0.8493923923204142 0.0 50-51 0.0 0.0 0.0 0.9873125444420476 0.0 52-53 0.0 0.0 0.0 1.11155053809832 0.0 54-55 0.0 0.0 0.0 1.2458753785064276 0.0 56-57 0.0 0.0 0.0 1.4015723496560484 0.0 58-59 0.0 0.0 0.0 1.5729488746674336 0.0 60-61 0.0 0.0 0.0 1.7832746099086791 0.0 62-63 0.0 0.0 0.0 1.982914279779168 0.0 64-65 0.0 0.0 0.0 2.17166814473926 0.0 66-67 0.0 0.0 0.0 2.3846903637656496 0.0 68-69 0.0 0.0 0.0 2.6184854949146317 0.0 70-71 0.0 0.0 0.0 2.8783466359866736 0.0 72-73 0.0 0.0 0.0 3.154786158848861 0.0 74-75 0.0 0.0 0.0 3.4477041937313744 0.0 76-77 0.0 0.0 0.0 3.7340308238057576 0.0 78-79 0.0 0.0 0.0 4.07868139945511 0.0 80-81 0.0 0.0 0.0 4.450396682725725 0.0 82-83 0.0 0.0 0.0 4.8061328028251165 0.0 84-85 0.0 0.0 0.0 5.212103417144045 0.0 86-87 0.0 0.0 0.0 5.624166087422002 0.0 88-89 0.0 0.0 0.0 6.012659492022403 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGTCAA 30 1.3192769E-4 63.150192 9 TAAGCGG 25 0.0050674523 56.863586 4 AAGCGGT 25 0.0050694584 56.857903 5 GGGATAG 105 2.1827873E-11 45.10728 7 GTATGGT 80 2.260822E-7 41.5586 1 CCTGTAT 60 8.401811E-5 39.4886 3 GATAGTC 60 8.426593E-5 39.46887 7 TACCTGG 125 1.382432E-10 37.909058 2 CGACCGT 25 0.0016356494 37.905266 18-19 AGTTCGA 25 0.0016380607 37.8939 14-15 TATGGTG 110 7.148083E-8 34.46278 2 TACATAG 70 2.0807597E-4 33.847374 2 GGGACTC 175 3.6379788E-12 32.47724 7 GTACAGA 60 0.0039506434 31.663694 1 TCGACCG 30 0.003999275 31.587725 18-19 AGTACTC 135 1.2238161E-8 31.587725 5 ATGGGTT 90 2.4517294E-5 31.587723 5 GGGAATG 210 0.0 31.575098 7 CATGGGT 410 0.0 31.205626 4 GGAATGA 185 9.094947E-12 30.721716 8 >>END_MODULE