##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939080_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 500652 Sequences flagged as poor quality 0 Sequence length 101 %GC 27 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01274138523365 33.0 31.0 34.0 30.0 34.0 2 32.163752466783315 33.0 31.0 34.0 30.0 34.0 3 32.15828759297876 34.0 31.0 34.0 30.0 34.0 4 35.839067855516404 37.0 35.0 37.0 35.0 37.0 5 35.84693359858744 37.0 35.0 37.0 35.0 37.0 6 35.9929471968553 37.0 35.0 37.0 35.0 37.0 7 35.92916836445276 37.0 35.0 37.0 35.0 37.0 8 35.95641283765969 37.0 35.0 37.0 35.0 37.0 9 37.497477289614345 39.0 37.0 39.0 35.0 39.0 10-11 37.42334196208145 39.0 37.0 39.0 34.0 39.0 12-13 37.27522011297268 39.0 37.0 39.0 34.0 39.0 14-15 38.2227355528391 40.0 37.5 41.0 33.0 41.0 16-17 38.38265202176362 40.0 38.0 41.0 34.0 41.0 18-19 38.53500535301966 40.0 38.0 41.0 34.0 41.0 20-21 38.5260440385737 40.0 37.5 41.0 34.0 41.0 22-23 38.28858168947692 40.0 37.0 41.0 34.0 41.0 24-25 38.06560245439947 40.0 36.0 41.0 33.5 41.0 26-27 37.703244968560995 40.0 35.5 41.0 33.0 41.0 28-29 37.32372086798814 39.0 35.0 41.0 33.0 41.0 30-31 36.65452050526114 37.5 35.0 40.0 33.0 41.0 32-33 35.81264630921279 36.0 35.0 40.0 31.0 41.0 34-35 35.39030504222494 35.0 35.0 40.0 31.0 41.0 36-37 35.0166063453257 35.0 35.0 40.0 30.0 41.0 38-39 34.71094293041873 35.0 35.0 40.0 29.5 41.0 40-41 34.34469351965038 35.0 34.5 40.0 26.0 41.0 42-43 33.94445243402603 35.0 33.5 39.0 24.5 41.0 44-45 33.40744469212147 35.0 33.0 39.0 23.0 41.0 46-47 32.73990216757348 35.0 33.0 39.0 20.5 41.0 48-49 32.16394321804367 35.0 33.0 38.0 19.0 40.0 50-51 31.9293081821305 35.0 33.0 38.0 15.5 40.0 52-53 31.67285060281393 35.0 32.5 38.0 14.0 40.0 54-55 31.49203139106605 35.0 32.0 38.0 11.0 40.0 56-57 31.30790648993712 35.0 32.0 38.0 7.5 40.0 58-59 30.961597077411056 35.0 31.0 37.0 2.0 40.0 60-61 30.52744321404888 35.0 31.0 36.5 2.0 40.0 62-63 30.0754675902623 35.0 30.5 36.0 2.0 39.0 64-65 29.566723991914543 35.0 29.0 35.5 2.0 39.0 66-67 29.099865775029365 35.0 29.0 35.0 2.0 39.0 68-69 28.57934353602902 34.0 27.5 35.0 2.0 38.0 70-71 28.03302992897262 33.5 26.0 35.0 2.0 37.5 72-73 27.40073444228726 33.0 26.0 35.0 2.0 36.5 74-75 26.818205060600977 33.0 24.5 35.0 2.0 36.0 76-77 24.88239036296669 30.0 20.5 33.5 2.0 35.0 78-79 25.930388773039958 32.5 23.0 35.0 2.0 35.0 80-81 25.923310003755105 33.0 22.0 35.0 2.0 35.0 82-83 25.45270367440857 33.0 20.0 35.0 2.0 35.0 84-85 24.86237745979243 32.5 14.5 35.0 2.0 35.0 86-87 23.984493220840022 31.5 4.5 35.0 2.0 35.0 88-89 23.073508145378426 31.0 2.0 34.5 2.0 35.0 90-91 22.293685034714734 30.0 2.0 34.0 2.0 35.0 92-93 21.610426004490144 29.0 2.0 34.0 2.0 35.0 94-95 20.87440976966036 29.0 2.0 34.0 2.0 35.0 96-97 19.866699823430245 28.0 2.0 34.0 2.0 35.0 98-99 18.564500890838346 26.0 2.0 33.0 2.0 35.0 100-101 13.236223963951009 2.0 2.0 26.5 2.0 30.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.04785173329520376 1101 2 0.2444725434517494 1101 3 -0.018446025080116613 1101 4 0.059838419576280444 1101 5 -0.012122953699538641 1101 6 0.019922633744492657 1101 7 0.07518277264955486 1101 8 0.1182227194158969 1101 9 0.17943339822004845 1101 10-11 0.24076488145356478 1101 12-13 0.08695534935937843 1101 14-15 0.536101831914003 1101 16-17 0.21184818570259978 1101 18-19 0.23801656224955536 1101 20-21 0.2835094839933916 1101 22-23 0.274569994127539 1101 24-25 0.25024476365417314 1101 26-27 0.23689241302434283 1101 28-29 0.30807478289697343 1101 30-31 0.3470961664868071 1101 32-33 0.6784915789414967 1101 34-35 0.6416407887159821 1101 36-37 0.580494070676643 1101 38-39 0.37327662153992236 1101 40-41 0.44798265352892486 1101 42-43 0.7139683902729885 1101 44-45 0.6596785434221744 1101 46-47 0.5342541760264865 1101 48-49 0.6865639017318159 1101 50-51 0.24221587199362915 1101 52-53 0.27619105781494113 1101 54-55 0.17275975927623932 1101 56-57 0.13884012206488094 1101 58-59 0.13290100159635898 1101 60-61 0.16537889376857962 1101 62-63 -0.11691388304895867 1101 64-65 -0.10385492286826192 1101 66-67 -0.41843566742350546 1101 68-69 -0.12477057013327197 1101 70-71 -0.5217272068086167 1101 72-73 -0.5351282755220659 1101 74-75 -0.7004056008914965 1101 76-77 -0.5087944801355988 1101 78-79 -0.3287180488003365 1101 80-81 0.04776092420347311 1101 82-83 -0.21603012616560235 1101 84-85 -0.30864925405435173 1101 86-87 -0.4875544075633691 1101 88-89 -0.546364626270595 1101 90-91 -0.5187441465900484 1101 92-93 -0.4154111820035453 1101 94-95 -0.9942989646174105 1101 96-97 -1.4819641006222923 1101 98-99 -1.759297699601433 1101 100-101 -1.8445577025972053 1102 1 0.33731373050368063 1102 2 0.24215684747408517 1102 3 0.14980673472194184 1102 4 0.22691747045815447 1102 5 0.30880713131505644 1102 6 0.2867034991169106 1102 7 0.29879018287668657 1102 8 0.3459222499323289 1102 9 0.5332907767253161 1102 10-11 0.23657759557614355 1102 12-13 0.4325491826292662 1102 14-15 0.5638108789892513 1102 16-17 0.46965176392870944 1102 18-19 0.3255847642543799 1102 20-21 0.16755021486510913 1102 22-23 0.32776755788848533 1102 24-25 0.30770891794932 1102 26-27 0.3463645612288886 1102 28-29 0.45404293414681973 1102 30-31 0.49834600787749395 1102 32-33 0.6971440342136717 1102 34-35 0.895669465279866 1102 36-37 0.7435127231319214 1102 38-39 0.6646736523735797 1102 40-41 0.7310526954017789 1102 42-43 1.10232329447296 1102 44-45 1.2383963457315232 1102 46-47 1.1753530213506806 1102 48-49 1.019564155506714 1102 50-51 0.9703436476566267 1102 52-53 1.1288747274000173 1102 54-55 0.7731594547463594 1102 56-57 0.6227254158093309 1102 58-59 0.3565718555488999 1102 60-61 0.5127332904187512 1102 62-63 0.29883284959918655 1102 64-65 0.6433059704193944 1102 66-67 1.1098760950431874 1102 68-69 1.0230533100849755 1102 70-71 0.9633000739933131 1102 72-73 0.5327882325352924 1102 74-75 0.7479987894143001 1102 76-77 0.6292114835745899 1102 78-79 0.6545486686213131 1102 80-81 0.6058595157527691 1102 82-83 0.761114271753442 1102 84-85 0.46890118370735223 1102 86-87 0.2473777076504362 1102 88-89 -0.08931602837692765 1102 90-91 0.02149503625476612 1102 92-93 0.1321874729894752 1102 94-95 -0.16618387769950616 1102 96-97 -0.1164489375719171 1102 98-99 -0.06674599296521677 1102 100-101 0.03259938406692342 1103 1 0.17585377169961447 1103 2 0.17259710297207675 1103 3 0.11023367436365561 1103 4 0.118637712640286 1103 5 0.1794924817925505 1103 6 0.12335539350552693 1103 7 0.22927205042552856 1103 8 0.1788706717952948 1103 9 0.30642707699787763 1103 10-11 0.3139604056379355 1103 12-13 0.3161836831806326 1103 14-15 0.15383595024989205 1103 16-17 0.41857817301678324 1103 18-19 0.34151437747036084 1103 20-21 0.47052495635024627 1103 22-23 0.5811953505772465 1103 24-25 0.6462671644174947 1103 26-27 0.5152782786414534 1103 28-29 0.4772650592742522 1103 30-31 0.3498013893559673 1103 32-33 0.40648385902694173 1103 34-35 0.43571586271656315 1103 36-37 0.5019287604163196 1103 38-39 0.5522170849516499 1103 40-41 0.6328404531364171 1103 42-43 0.49713583218387924 1103 44-45 0.5643045287413244 1103 46-47 0.5486287219935875 1103 48-49 0.4071778084205988 1103 50-51 0.25184136939683555 1103 52-53 0.030571458773422933 1103 54-55 -0.30279294106746946 1103 56-57 -0.24281044337549318 1103 58-59 -0.09398262502867283 1103 60-61 -0.04791627369501228 1103 62-63 0.09358661028828053 1103 64-65 0.38622411951537217 1103 66-67 0.29955162507668476 1103 68-69 -0.020467693597691294 1103 70-71 -0.10057442406132466 1103 72-73 -0.16848925742580434 1103 74-75 -0.4025302037821312 1103 76-77 -0.1321043935034112 1103 78-79 0.03611296964288968 1103 80-81 0.05822672129549389 1103 82-83 0.46320579745928825 1103 84-85 0.13921150557656858 1103 86-87 -0.3020053936957119 1103 88-89 0.2831846478188993 1103 90-91 0.6254133309929237 1103 92-93 0.19177853055974836 1103 94-95 -0.015176345918302303 1103 96-97 -0.06856647080954303 1103 98-99 -0.8251880377813983 1103 100-101 -0.19428772243912107 1104 1 -0.04510702718705417 1104 2 0.05548259222531016 1104 3 0.0014791414699359962 1104 4 0.08020974761652155 1104 5 0.048620919184998 1104 6 -0.07322878738125382 1104 7 0.09143217692256655 1104 8 0.14433837664218885 1104 9 0.23236944502644974 1104 10-11 0.17833522793852552 1104 12-13 0.026470047338470692 1104 14-15 -0.1359998929057511 1104 16-17 0.0969210418459383 1104 18-19 0.2605832076221546 1104 20-21 0.384303955646935 1104 22-23 0.27594143370814805 1104 24-25 0.2063308119815801 1104 26-27 0.279478020260278 1104 28-29 0.21723782450153806 1104 30-31 0.09496422436611596 1104 32-33 0.2714148493052235 1104 34-35 0.20639758000366015 1104 36-37 0.11853947774830687 1104 38-39 0.6359440588491694 1104 40-41 0.6963957382286239 1104 42-43 0.5447692501478585 1104 44-45 0.6840577198486244 1104 46-47 0.6087048396917041 1104 48-49 0.4722644259715594 1104 50-51 0.007476622964539104 1104 52-53 -0.03777734583952963 1104 54-55 0.062352535516112084 1104 56-57 0.19640762934035294 1104 58-59 -0.033529909282886905 1104 60-61 0.05994789162903302 1104 62-63 0.26350216254063596 1104 64-65 0.3193343205838097 1104 66-67 -0.07969009101327984 1104 68-69 -0.4374714695542785 1104 70-71 -1.0690000446998944 1104 72-73 -0.9512271002921402 1104 74-75 -1.4434596776921147 1104 76-77 -1.2527155298319066 1104 78-79 -1.0113907589271882 1104 80-81 -0.9143888643768214 1104 82-83 -1.0726982124370075 1104 84-85 -1.0898349282145006 1104 86-87 -1.6494856784138143 1104 88-89 -1.068515530254615 1104 90-91 -1.3030554861899226 1104 92-93 -1.6249803319645046 1104 94-95 -1.4760874637909964 1104 96-97 -1.9092879428839247 1104 98-99 -1.9577330923756477 1104 100-101 -1.0670275106463638 1105 1 -0.11491791290443132 1105 2 0.025042674477553817 1105 3 0.01414165788039412 1105 4 -0.06615501379080513 1105 5 -0.07188238172739858 1105 6 0.10705326064314136 1105 7 -0.12594337420981105 1105 8 -0.017912172725623066 1105 9 -0.020189173813456307 1105 10-11 0.057413136999919345 1105 12-13 0.08491478352428317 1105 14-15 0.09704264016219355 1105 16-17 0.20063274490969718 1105 18-19 0.012955958978508875 1105 20-21 0.16610023547395514 1105 22-23 0.06444541221340216 1105 24-25 0.010831157836257432 1105 26-27 0.17522283126363192 1105 28-29 -0.14039709411674295 1105 30-31 0.24023495564879482 1105 32-33 0.25405388524117 1105 34-35 0.1761999958103999 1105 36-37 0.49595634322259485 1105 38-39 0.442333665327709 1105 40-41 0.611204906436349 1105 42-43 0.21495798799000454 1105 44-45 0.06761527053868832 1105 46-47 0.05445270927390311 1105 48-49 0.5111366103144661 1105 50-51 0.037123662966045856 1105 52-53 0.4708196134625524 1105 54-55 0.2576733804367528 1105 56-57 0.09374054858916381 1105 58-59 -0.2382288274870774 1105 60-61 -0.44955252480824015 1105 62-63 -0.7501720376409935 1105 64-65 -0.2626661781998152 1105 66-67 -0.15580870740632236 1105 68-69 -0.573764095555962 1105 70-71 -0.6209017749144188 1105 72-73 -0.5052792467086604 1105 74-75 -0.35109294938331814 1105 76-77 -0.26042079576357224 1105 78-79 -0.21914119771561147 1105 80-81 0.0647375113954709 1105 82-83 0.06006763688327865 1105 84-85 -0.2031458633396923 1105 86-87 -0.12938140440279255 1105 88-89 0.4584376076120016 1105 90-91 0.4830816228413539 1105 92-93 0.7004101073885813 1105 94-95 0.5147838547388126 1105 96-97 -0.13259007871305784 1105 98-99 -0.9116497202218881 1105 100-101 -0.8507177566031583 1106 1 -0.012615917148309563 1106 2 0.09347399976737591 1106 3 -0.0909155493842917 1106 4 0.09953575034324302 1106 5 0.143728278058326 1106 6 -0.030116574753058956 1106 7 0.09167580168421097 1106 8 0.019096508789886002 1106 9 0.2942735599423756 1106 10-11 -0.07600668067407668 1106 12-13 0.12226845721838941 1106 14-15 0.11613299503154195 1106 16-17 -0.013595583201500006 1106 18-19 -0.02321387169176603 1106 20-21 0.05875568280594479 1106 22-23 0.12238755749763897 1106 24-25 0.12961243752095442 1106 26-27 0.19720781228905793 1106 28-29 0.24581219798766796 1106 30-31 0.151557787987322 1106 32-33 -0.05338849397612222 1106 34-35 -0.17251857091250855 1106 36-37 -0.092160802888678 1106 38-39 -0.1648880134136803 1106 40-41 -0.11119926371568312 1106 42-43 0.023203773650614323 1106 44-45 -0.039854519016081724 1106 46-47 -0.1802341276204089 1106 48-49 -0.45306926967551675 1106 50-51 -0.4750526705704985 1106 52-53 -0.25692484657743364 1106 54-55 -0.07902244515788581 1106 56-57 0.1968152323069532 1106 58-59 -0.05517503233681609 1106 60-61 -0.3374064371613237 1106 62-63 0.1428118611486937 1106 64-65 0.1489024019919647 1106 66-67 0.2777519426912676 1106 68-69 -0.060094371218891496 1106 70-71 0.03531351910870839 1106 72-73 0.045725247641502875 1106 74-75 -0.04552439754706583 1106 76-77 0.05743987465547562 1106 78-79 -0.06655068452793245 1106 80-81 0.043766240264702105 1106 82-83 -0.12048614645217981 1106 84-85 -0.20860902715426732 1106 86-87 -0.00444461358620174 1106 88-89 -0.05943582279173043 1106 90-91 0.08234248891350049 1106 92-93 -0.09314279219979227 1106 94-95 -0.23334349936021326 1106 96-97 -0.9450750148422458 1106 98-99 -0.9635332357394475 1106 100-101 -0.7657409845012104 1107 1 -0.03642205602280413 1107 2 -0.026061159116018473 1107 3 0.07118265609786079 1107 4 0.05579867724633658 1107 5 -0.015274349743172877 1107 6 0.08454362253842618 1107 7 0.16001239875006235 1107 8 0.20286375803662793 1107 9 0.07235044950591885 1107 10-11 0.1069992857652764 1107 12-13 0.2192494664225748 1107 14-15 0.3319360337423163 1107 16-17 0.16085512373672373 1107 18-19 0.2482201374711508 1107 20-21 0.1937939533181563 1107 22-23 0.09399996365577579 1107 24-25 0.20175544862375006 1107 26-27 0.1391668772870176 1107 28-29 0.2703796196695478 1107 30-31 0.411141467184585 1107 32-33 0.2062387526107088 1107 34-35 0.17553442537738562 1107 36-37 0.43283999966849507 1107 38-39 0.12308555264593934 1107 40-41 0.28943269273526795 1107 42-43 0.10863483857519896 1107 44-45 0.014116304591020423 1107 46-47 -0.025535499305270548 1107 48-49 -0.2880016026404384 1107 50-51 -0.29753390219767084 1107 52-53 -0.03854903886096395 1107 54-55 -0.011919493123290437 1107 56-57 -0.16921504473578253 1107 58-59 -0.1299527239989331 1107 60-61 -0.41171864566109306 1107 62-63 -0.9242544025578354 1107 64-65 -0.9516052727476669 1107 66-67 -0.7285355195312206 1107 68-69 -0.6168672658821102 1107 70-71 -0.578392781653033 1107 72-73 -0.5114282884254173 1107 74-75 -1.3699730407668333 1107 76-77 -1.1235574058222078 1107 78-79 -0.9476621574267234 1107 80-81 -0.6407049662768287 1107 82-83 -0.8007083859994459 1107 84-85 -0.6917920147765422 1107 86-87 -0.9059754340748007 1107 88-89 -0.9668849292016439 1107 90-91 -0.6917284938292276 1107 92-93 -1.2378461441371655 1107 94-95 -1.5829053031418638 1107 96-97 -1.5002198649474963 1107 98-99 -1.6461700258776233 1107 100-101 -1.2001468399583715 1108 1 -0.05488402280518301 1108 2 -0.036927792721670016 1108 3 0.004216700243013349 1108 4 0.10498998741955745 1108 5 -0.032061515109148786 1108 6 0.024385625687926904 1108 7 0.01675815084465171 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2316 68-69 0.5393793376050304 2316 70-71 0.5940203465046316 2316 72-73 0.9465303328445565 2316 74-75 0.3992078342800589 2316 76-77 0.26185345390312875 2316 78-79 0.21002018491372354 2316 80-81 0.176547741291742 2316 82-83 0.28819172233835744 2316 84-85 0.3535152754109987 2316 86-87 0.533805926914475 2316 88-89 -0.007159244727606762 2316 90-91 -0.12692390507462292 2316 92-93 -0.04106226929011925 2316 94-95 0.0681119853276968 2316 96-97 -0.37802954803312616 2316 98-99 -0.11914167222656857 2316 100-101 0.15268028586424265 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 6.0 4 28.0 5 100.0 6 286.0 7 433.0 8 612.0 9 949.0 10 1471.0 11 2059.0 12 2658.0 13 3542.0 14 4073.0 15 5355.0 16 7051.0 17 9595.0 18 8977.0 19 6265.0 20 6086.0 21 6884.0 22 7537.0 23 8372.0 24 9404.0 25 10558.0 26 11221.0 27 12645.0 28 14600.0 29 17199.0 30 20167.0 31 23037.0 32 27749.0 33 35652.0 34 59760.0 35 93259.0 36 42310.0 37 31481.0 38 9121.0 39 147.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.05041083365748 22.325170128042153 16.52477633726456 12.099642701035807 2 16.360865431477354 28.04622771905435 38.072153911299665 17.52075293816863 3 19.387518675646955 37.42060353299298 30.637249027268442 12.554628764091625 4 10.346308413828368 23.34056390466831 36.74128935867629 29.57183832282703 5 8.631344726476673 44.65596861692353 30.641044078521606 16.071642578078187 6 20.52723248883456 42.084921262673475 18.21444835933942 19.173397889152547 7 19.774014684850954 44.90424486469644 20.354457787045693 14.967282663406916 8 25.600816535238053 32.95722378019063 20.016698225513927 21.425261459057392 9 28.001086583095642 20.853606896606824 21.37492709506803 29.7703794252295 10-11 22.088496600433036 33.99846600033556 23.133633741600953 20.77940365763045 12-13 26.874056230675198 34.30816215654786 26.244876680808225 12.572904931968711 14-15 22.359699469787685 32.741434359635555 32.021268414865915 12.877597755710843 16-17 13.029708942844218 37.16728870317124 31.932993834897495 17.87000851908705 18-19 12.44916628716154 32.02833505109338 41.87649305305881 13.646005608686274 20-21 14.867712502896225 30.336141671260673 42.59685370277159 12.199292123071515 22-23 13.64400821328987 31.557449086391347 42.75195145530228 12.046591245016499 24-25 13.188602062909965 32.69716289957895 42.9418038877304 11.172431149780685 26-27 12.57549200261669 32.325208611106945 44.03389712014302 11.065402266133342 28-29 11.549961949541697 33.85402206327362 43.624301159889825 10.971714827294862 30-31 11.578701373409075 34.483733211891696 42.6548780390371 11.282687375662137 32-33 11.252737682799314 35.24058152227648 42.46167244080962 11.045008354114588 34-35 11.850199715733446 34.600413714712644 42.188812522910624 11.360574046643288 36-37 11.824342548213798 34.5276316551822 42.431837388693204 11.2161884079108 38-39 11.667262662242315 34.870535776715414 42.529511769747266 10.932689791295001 40-41 11.019499464146774 35.23656946066098 42.600980616396015 11.142950458796234 42-43 10.877761763933293 35.9264774769106 41.238512730007585 11.957248029148527 44-45 11.391589639106531 35.37684828691705 40.83474404877829 12.396818025198124 46-47 11.920511281409519 35.9529947096077 40.070252254933585 12.056241754049193 48-49 11.841948882969746 35.896076087293046 39.93883773723597 12.323137292501231 50-51 11.529046826266333 36.250283382452956 40.07801880960395 12.142650981676766 52-53 11.517581074279141 36.89059466455742 39.22075613400126 12.37106812716218 54-55 10.926252167174006 36.234650016378644 39.54163770443342 13.297460112013933 56-57 12.091269258694439 36.472149733197114 38.48233448283783 12.954246525270625 58-59 12.131211393359532 35.915448648486695 38.93666638369695 13.016673574456822 60-61 11.770880356664895 36.50052532967933 39.34249897131261 12.38609534234317 62-63 11.981116246739187 36.35442092353418 39.2596905573244 12.404772272402235 64-65 11.70171541798832 36.67285213208798 39.12303549884547 12.502396951078229 66-67 11.812820525624572 36.24672296099512 38.55086214214734 13.389594371232965 68-69 11.493053232087266 36.386262468077625 38.76277519158418 13.35790910825093 70-71 12.211125536802157 36.222310995705584 38.207929691401176 13.35863377609108 72-73 11.791823462205283 35.5064995246199 38.60955314270192 14.092123870472903 74-75 11.455262337911364 36.66808481739811 37.922948475188356 13.953704369502168 76-77 11.742227357781438 35.293453228205415 38.72477089897164 14.239548515041504 78-79 11.459359249174438 35.12942271694811 39.12217896034313 14.289039073534319 80-81 11.901088090922256 35.78915304680439 38.233387762847116 14.076371099426243 82-83 11.58269616420188 35.166942307231366 38.46883663702532 14.78152489154143 84-85 11.482726524611907 34.834276103960434 38.234741896566874 15.448255474860781 86-87 11.962508821982881 34.93815115230591 38.02989377632849 15.069446249382711 88-89 12.109109500466152 34.28327970086112 37.954748228123464 15.652862570549257 90-91 12.10905113601617 33.729700033183725 38.331610653679235 15.829638177120875 92-93 12.240338598467599 34.44688126682806 38.04918386424103 15.263596270463317 94-95 11.96399894537523 33.94473606417232 38.04169363150452 16.04957135894793 96-97 11.67200305202626 33.64040019894098 38.08241669346511 16.60518005556765 98-99 12.319272626313166 33.54016424631555 37.32963414597609 16.810928981395204 100-101 12.60061919504644 32.04594027763907 38.40107859782283 16.952361929491662 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 18274.0 1 11908.5 2 7544.0 3 9266.5 4 7498.5 5 6839.0 6 11178.0 7 20473.5 8 27815.5 9 26450.5 10 17663.5 11 10003.5 12 7879.5 13 7244.5 14 6391.0 15 5326.0 16 4413.5 17 3783.0 18 3275.5 19 2894.0 20 2682.5 21 2576.5 22 2584.0 23 2612.5 24 2655.5 25 2832.5 26 3148.0 27 3502.0 28 3780.0 29 4180.0 30 4875.5 31 5625.5 32 6863.0 33 8200.0 34 9161.0 35 10005.0 36 11444.5 37 12476.5 38 12028.5 39 11175.0 40 10325.0 41 9385.0 42 8338.0 43 8517.0 44 8874.0 45 8567.0 46 8581.0 47 11090.5 48 18669.0 49 18865.5 50 12029.5 51 8051.0 52 5086.0 53 3828.5 54 3591.0 55 3469.5 56 3157.5 57 2939.0 58 2814.0 59 2536.0 60 2133.5 61 1801.0 62 1529.5 63 1322.5 64 1091.0 65 885.0 66 737.5 67 636.5 68 473.0 69 335.0 70 307.0 71 260.5 72 207.5 73 195.5 74 158.0 75 111.0 76 91.0 77 69.5 78 53.5 79 42.0 80 30.5 81 24.5 82 17.0 83 13.0 84 11.0 85 5.5 86 1.5 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3815025207129903 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 6.990883887410816E-4 16-17 0.002297004705863554 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0038949210229860263 28-29 1.9973953964030902E-4 30-31 0.0 32-33 9.986976982015451E-5 34-35 0.013082939846440243 36-37 0.024168484296477392 38-39 0.017776819027987504 40-41 0.009687367672554988 42-43 0.008688669974353443 44-45 0.0038949210229860263 46-47 0.005492837340108499 48-49 0.003295702404065099 50-51 6.990883887410816E-4 52-53 0.0 54-55 0.0 56-57 2.9960930946046355E-4 58-59 0.0018975256265829359 60-61 0.0027963535549643263 62-63 0.0019973953964030904 64-65 0.0031958326342449443 66-67 0.0018975256265829359 68-69 0.004693879181547262 70-71 3.9947907928061804E-4 72-73 0.0 74-75 0.0 76-77 0.08708643928317475 78-79 0.01657838179014565 80-81 8.988279283813908E-4 82-83 0.0 84-85 0.0 86-87 0.09607471856698864 88-89 0.3769085113012631 90-91 0.38220160910173134 92-93 0.0 94-95 0.0 96-97 1.9973953964030902E-4 98-99 2.9960930946046355E-4 100-101 3.9947907928061804E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 500652.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.295951752569387 #Duplication Level Percentage of deduplicated Percentage of total 1 70.6448059843928 18.576724097352184 2 12.630722283474618 6.642737275327032 3 5.221390459839427 4.119042948397911 4 2.896242743772909 3.046378378159264 5 1.7400757309545256 2.2878473733498557 6 1.2015430234132147 1.8957430423386143 7 0.8404756826026425 1.5470775599248836 8 0.6495858755955453 1.36651830750488 9 0.5036771211180852 1.1920202348214792 >10 3.211122306616699 16.176936068870546 >50 0.287914159629037 5.217182121481836 >100 0.1390192380266854 6.518830806383457 >500 0.017472363249255544 3.382968152057851 >1k 0.010635351543025112 6.193418635158053 >5k 0.00227900390207681 4.433915624628052 >10k+ 0.0030386718694357467 17.4026593742441 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27973 5.587314142358365 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 21014 4.197326686001454 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20394 4.073488171424462 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17736 3.5425804750605208 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7456 1.489258007558144 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7370 1.4720804071490776 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7370 1.4720804071490776 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4776 0.953956041322116 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3892 0.7773862882800827 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3663 0.731645933702452 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3522 0.7034826586131684 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2161 0.4316371451627078 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1985 0.3964829861860134 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1956 0.3906905395364445 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1548 0.3091968073631984 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1406 0.2808337927342745 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1314 0.2624577550873661 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1299 0.25946166199276144 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1274 0.2544681735017537 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1206 0.24088588480621267 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1002 0.20013901871958964 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 998 0.19934006056102843 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 972 0.1941468325303804 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 968 0.19334787437181913 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 914 0.18256193923124248 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 896 0.1789666275177169 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 871 0.17397313902670916 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 866 0.17297444132850762 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 852 0.1701780877735433 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 836 0.16698225513929837 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 782 0.15619631999872166 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 750 0.14980465473023177 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 750 0.14980465473023177 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 665 0.1328267938608055 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 660 0.13182809616260396 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 650 0.12983070076620087 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 638 0.12743382629051717 No Hit GTACATGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 608 0.1214416401013079 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 604 0.12064268194274666 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 550 0.10985674680216997 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 532 0.10626143508864441 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 532 0.10626143508864441 No Hit ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT 529 0.10566221646972347 TruSeq Adapter, Index 3 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 512 0.10226664429583822 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.996093094604636E-4 9.986976982015452E-5 16-17 0.0 0.0 0.0 3.994790792806181E-4 1.9973953964030905E-4 18-19 0.0 0.0 0.0 3.994790792806181E-4 1.9973953964030905E-4 20-21 0.0 0.0 0.0 0.0018975256265829359 1.9973953964030905E-4 22-23 0.0 0.0 0.0 0.004494139641906953 1.9973953964030905E-4 24-25 0.0 0.0 0.0 0.008788539744173597 1.9973953964030905E-4 26-27 0.0 0.0 0.0 0.017077730639246422 1.9973953964030905E-4 28-29 0.0 0.0 0.0 0.032357805421730064 1.9973953964030905E-4 30-31 0.0 0.0 0.0 0.07050805749302909 1.9973953964030905E-4 32-33 0.0 0.0 0.0 0.11784632838778233 1.9973953964030905E-4 34-35 0.0 0.0 0.0 0.16778121329785958 1.9973953964030905E-4 36-37 0.0 0.0 0.0 0.228801642657974 1.9973953964030905E-4 38-39 0.0 0.0 0.0 0.31319159815600456 1.9973953964030905E-4 40-41 0.0 0.0 0.0 0.4022754328355824 1.9973953964030905E-4 42-43 0.0 0.0 0.0 0.4901608302773184 1.9973953964030905E-4 44-45 0.0 0.0 0.0 0.5947244792790202 1.9973953964030905E-4 46-47 0.0 0.0 0.0 0.7186628636258319 1.9973953964030905E-4 48-49 0.0 0.0 0.0 0.8463962992258096 1.9973953964030905E-4 50-51 0.0 0.0 0.0 0.9835174931888817 1.9973953964030905E-4 52-53 0.0 0.0 0.0 1.10515887282983 1.9973953964030905E-4 54-55 0.0 0.0 0.0 1.2385848853095562 1.9973953964030905E-4 56-57 0.0 0.0 0.0 1.3926839401420548 1.9973953964030905E-4 58-59 0.0 0.0 0.0 1.560165544130454 1.9973953964030905E-4 60-61 0.0 0.0 0.0 1.768294144435656 1.9973953964030905E-4 62-63 0.0 0.0 0.0 1.9634396746642377 1.9973953964030905E-4 64-65 0.0 0.0 0.0 2.1481987488315237 1.9973953964030905E-4 66-67 0.0 0.0 0.0 2.3556282607479844 1.9973953964030905E-4 68-69 0.0 0.0 0.0 2.582332638239736 1.9973953964030905E-4 70-71 0.0 0.0 0.0 2.834903286114906 1.9973953964030905E-4 72-73 0.0 0.0 0.0 3.1024543994631 1.9973953964030905E-4 74-75 0.0 0.0 0.0 3.383787541046475 1.9973953964030905E-4 76-77 0.0 0.0 0.0 3.659627845289742 1.9973953964030905E-4 78-79 0.0 0.0 0.0 3.9882992577678706 1.9973953964030905E-4 80-81 0.0 0.0 0.0 4.342237722010498 1.9973953964030905E-4 82-83 0.0 0.0 0.0 4.6789985858440595 1.9973953964030905E-4 84-85 0.0 0.0 0.0 5.0619991531043524 1.9973953964030905E-4 86-87 0.0 0.0 0.0 5.446298027372307 1.9973953964030905E-4 88-89 0.0 0.0 0.0 5.8038318033284595 1.9973953964030905E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGTATG 55 4.983716E-5 43.145866 7 AGGTAAG 45 9.659376E-4 42.187065 2 GGTAAGG 45 9.659376E-4 42.187065 3 GGATGTA 95 1.9386789E-8 39.966698 8 ATGGGTA 120 8.731149E-11 39.550377 5 GTAAGGT 60 8.324799E-5 39.550377 4 TCATAGG 25 0.0016223415 37.96836 94-95 TACACGG 50 0.0016215665 37.96836 4 CATGGGT 345 0.0 37.142963 4 GGATATG 80 1.05688E-5 35.720196 1 GTAAGCG 110 0.0 34.516693 94-95 TGGGTAT 85 1.639691E-5 33.501495 6 TAAGGTG 85 1.639691E-5 33.501495 5 GGGATAG 100 1.3057215E-6 33.222317 7 TGGGAGT 475 0.0 31.973358 6 CCCTATA 135 1.2039891E-8 31.640303 2 CTAGATA 60 0.0039649736 31.6403 4 ATTCCCG 30 0.0039668516 31.6403 66-67 GGGAACT 300 0.0 31.6403 7 AGACCGG 30 0.0039668516 31.6403 60-61 >>END_MODULE