##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939079_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1409844 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.339924133450225 33.0 31.0 34.0 31.0 34.0 2 32.40314531253103 34.0 31.0 34.0 31.0 34.0 3 32.43817542933828 34.0 31.0 34.0 31.0 34.0 4 35.89802134136826 37.0 35.0 37.0 35.0 37.0 5 35.96606007473167 37.0 35.0 37.0 35.0 37.0 6 36.08608399227148 37.0 37.0 37.0 35.0 37.0 7 36.03411441265842 37.0 37.0 37.0 35.0 37.0 8 36.0397561716048 37.0 37.0 37.0 35.0 37.0 9 37.738410774525406 39.0 38.0 39.0 35.0 39.0 10-11 37.67806615483699 39.0 38.0 39.0 35.0 39.0 12-13 37.59742850982094 39.0 38.0 39.0 35.0 39.0 14-15 38.854019664587 40.0 38.0 41.0 35.5 41.0 16-17 38.84040503772049 40.0 38.0 41.0 35.5 41.0 18-19 38.86547341407986 40.0 38.5 41.0 35.0 41.0 20-21 38.803095945367005 40.0 38.5 41.0 35.0 41.0 22-23 38.700262227593974 40.0 38.0 41.0 35.0 41.0 24-25 38.69297525116254 40.5 38.0 41.0 35.0 41.0 26-27 38.573944351289924 40.0 38.0 41.0 35.0 41.0 28-29 38.44430802273159 40.0 38.0 41.0 35.0 41.0 30-31 38.18913262743963 40.0 37.5 41.0 34.5 41.0 32-33 37.873018575104766 40.0 36.5 41.0 34.0 41.0 34-35 37.52432822354814 40.0 36.0 41.0 33.5 41.0 36-37 37.23688259126541 40.0 35.5 41.0 33.0 41.0 38-39 36.98164619631676 40.0 35.0 41.0 32.5 41.0 40-41 36.81702550069369 40.0 35.0 41.0 32.5 41.0 42-43 36.54925509488993 39.5 35.0 41.0 31.0 41.0 44-45 36.1677235211839 39.0 35.0 41.0 30.5 41.0 46-47 35.63395382751567 39.0 35.0 41.0 28.5 41.0 48-49 35.39245725058943 39.0 35.0 41.0 26.0 41.0 50-51 34.33244103602952 38.0 34.0 40.0 24.5 40.5 52-53 34.51736929759605 38.5 34.5 39.5 24.0 40.5 54-55 35.0002439986268 39.0 35.0 41.0 25.0 41.0 56-57 34.8759951455622 39.0 35.0 41.0 24.5 41.0 58-59 34.7008676137218 39.0 35.0 41.0 24.0 41.0 60-61 34.39404182306695 38.0 35.0 40.5 23.0 41.0 62-63 34.062460101968725 38.0 35.0 40.0 22.0 41.0 64-65 33.70339874482567 37.0 34.0 40.0 20.0 41.0 66-67 33.30066127883653 36.5 34.0 39.5 18.5 41.0 68-69 32.896513373110785 36.0 34.0 39.0 14.0 41.0 70-71 32.46988815783874 35.5 33.0 39.0 7.5 41.0 72-73 32.017978939513874 35.0 33.0 38.0 4.5 40.0 74-75 31.580532668862656 35.0 33.0 37.0 2.0 39.5 76-77 31.162750275917052 35.0 32.0 37.0 2.0 39.0 78-79 30.73103371720559 35.0 32.0 36.0 2.0 39.0 80-81 30.294644655720774 35.0 31.0 36.0 2.0 37.5 82-83 29.857075676457818 35.0 31.0 35.5 2.0 37.0 84-85 29.46186244719274 35.0 31.0 35.0 2.0 37.0 86-87 29.119073103123466 34.5 30.0 35.0 2.0 36.0 88-89 28.707796039845544 34.0 29.5 35.0 2.0 36.0 90-91 28.338605902497015 34.0 29.0 35.0 2.0 36.0 92-93 27.93326211978063 34.0 29.0 35.0 2.0 35.0 94-95 27.487893696040132 34.0 28.0 35.0 2.0 35.0 96-97 26.956215368508857 34.0 26.5 35.0 2.0 35.0 98-99 26.36594261492761 34.0 25.0 35.0 2.0 35.0 100-101 24.08981667475267 31.5 11.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22233003028405562 1101 2 0.2596721324605582 1101 3 0.3337421973767931 1101 4 0.19269816441417476 1101 5 0.2610104973296785 1101 6 0.2242140980470353 1101 7 0.1368702603124632 1101 8 0.2792958496569611 1101 9 0.3218270353636896 1101 10-11 0.328416680921471 1101 12-13 0.2819521856627958 1101 14-15 0.26980966627167646 1101 16-17 0.4100430783384539 1101 18-19 0.3729054564199785 1101 20-21 0.45988535510206674 1101 22-23 0.45085341305641435 1101 24-25 0.39943445641112874 1101 26-27 0.45660219395859514 1101 28-29 0.41988629949589296 1101 30-31 0.37772965520101565 1101 32-33 0.4765991697877823 1101 34-35 0.3292223089368278 1101 36-37 0.33047305382559955 1101 38-39 0.4693620554748179 1101 40-41 0.5033618611189397 1101 42-43 0.5455007389207438 1101 44-45 0.5104126887006402 1101 46-47 0.5429551756377009 1101 48-49 0.7003039296275588 1101 50-51 0.5505051206741243 1101 52-53 0.7011662890991133 1101 54-55 0.7643116445471563 1101 56-57 0.6064178434286376 1101 58-59 0.6772394202532297 1101 60-61 0.6109840311701902 1101 62-63 0.5787747486415569 1101 64-65 0.6896130714436381 1101 66-67 0.4537153205501383 1101 68-69 0.5610773666588642 1101 70-71 0.5467062487055401 1101 72-73 0.5741647732526545 1101 74-75 0.4370111467608844 1101 76-77 0.48067352145074693 1101 78-79 0.49197710586030396 1101 80-81 0.629346580710731 1101 82-83 0.4453324962199652 1101 84-85 0.399403187063033 1101 86-87 0.2567894267281936 1101 88-89 0.26203780034764534 1101 90-91 0.2894462816743193 1101 92-93 0.34124736506618447 1101 94-95 0.18612657229760288 1101 96-97 0.1436437097847083 1101 98-99 0.08142187187839411 1101 100-101 -0.011864417522588155 1102 1 0.2111230766179233 1102 2 0.2693832774645202 1102 3 0.27033220441870043 1102 4 0.31820880548771413 1102 5 0.2679554208351149 1102 6 0.27280602300252355 1102 7 0.21333135460236718 1102 8 0.24210075727540925 1102 9 0.4012735998190422 1102 10-11 0.34421795283694934 1102 12-13 0.347001762499211 1102 14-15 0.5380959271666654 1102 16-17 0.35945435127162995 1102 18-19 0.5157271799330587 1102 20-21 0.4776707933022877 1102 22-23 0.4143877835292855 1102 24-25 0.2668641448662896 1102 26-27 0.29383840400291916 1102 28-29 0.33463408498190006 1102 30-31 0.38171062359710106 1102 32-33 0.4148895492792164 1102 34-35 0.3968946735644394 1102 36-37 0.4449075391137427 1102 38-39 0.3704829557467306 1102 40-41 0.4574757105996028 1102 42-43 0.3675944475768276 1102 44-45 0.27444784771739705 1102 46-47 0.4190762637394698 1102 48-49 0.5133333109979787 1102 50-51 0.5875250190274315 1102 52-53 0.5655531339058868 1102 54-55 0.5489797423634784 1102 56-57 0.5020510383598236 1102 58-59 0.35496129683057376 1102 60-61 0.48432762798213247 1102 62-63 0.5169493566406587 1102 64-65 0.6913045646636604 1102 66-67 0.8035624270482984 1102 68-69 0.6510867991326634 1102 70-71 0.6120782098746176 1102 72-73 0.4524366839467291 1102 74-75 0.3610186025815949 1102 76-77 0.4671227927050623 1102 78-79 0.4991146403441604 1102 80-81 0.4739074720760925 1102 82-83 0.38319803873064373 1102 84-85 0.5060560522196376 1102 86-87 0.33208631742692774 1102 88-89 0.5045719051266495 1102 90-91 0.5543014033846454 1102 92-93 0.2373614044257586 1102 94-95 0.11277389135085869 1102 96-97 -0.05131070220197742 1102 98-99 -0.20016513330092423 1102 100-101 0.02092726004390144 1103 1 0.0716279464515921 1103 2 0.07323812405776664 1103 3 0.1104780663586169 1103 4 0.06272732923489599 1103 5 0.03230155260363432 1103 6 0.012878900699305973 1103 7 -0.07675837073448832 1103 8 -0.041335149107645464 1103 9 0.03324722427249327 1103 10-11 0.07743057804554354 1103 12-13 0.05020194842256842 1103 14-15 0.07512311113357129 1103 16-17 0.19022779779268717 1103 18-19 0.20402086692921273 1103 20-21 0.131167777374948 1103 22-23 0.2134790098377124 1103 24-25 0.14283221825540693 1103 26-27 0.11715593740186847 1103 28-29 0.07904905900456782 1103 30-31 0.07888694580388744 1103 32-33 0.23737369052238222 1103 34-35 0.09055563245486553 1103 36-37 0.16361521191525696 1103 38-39 0.12995026399993748 1103 40-41 4.058947782752398E-4 1103 42-43 0.22794415930238188 1103 44-45 0.02387715888198727 1103 46-47 0.20245723216578426 1103 48-49 0.48898780518920404 1103 50-51 0.427801733191572 1103 52-53 0.28699538512351097 1103 54-55 0.1574692215782889 1103 56-57 0.051085994882264174 1103 58-59 0.021326082199628615 1103 60-61 0.19968075584973377 1103 62-63 0.01687111167197486 1103 64-65 -0.045563931847610206 1103 66-67 -0.06037989409036726 1103 68-69 -0.23259189972342398 1103 70-71 0.056760348845678266 1103 72-73 -0.17211165945745677 1103 74-75 -0.018905336649698512 1103 76-77 -0.12541803119115258 1103 78-79 -0.23535290097917283 1103 80-81 -0.13290123861996506 1103 82-83 -0.14041629281759427 1103 84-85 -0.10181279456272918 1103 86-87 -0.05676886878277898 1103 88-89 -0.18747620853698166 1103 90-91 -0.29478269763726317 1103 92-93 -0.32783230348011116 1103 94-95 -0.5505832906049868 1103 96-97 -0.46902063511774017 1103 98-99 -0.6485883806469239 1103 100-101 -0.7220063868908255 1104 1 0.16209005356596862 1104 2 0.2516866731671925 1104 3 0.2477413636327057 1104 4 0.07388635461270354 1104 5 0.0901480179110905 1104 6 0.2530503695854236 1104 7 0.12903752301001248 1104 8 0.0964496098952381 1104 9 0.24271507769062595 1104 10-11 0.3112774063312642 1104 12-13 0.1568325643608901 1104 14-15 0.28907131771421035 1104 16-17 0.21209276476677985 1104 18-19 0.17180271188126284 1104 20-21 0.2903446091370796 1104 22-23 0.29601822171476755 1104 24-25 0.30989807580433393 1104 26-27 0.2773232908302177 1104 28-29 0.32344334541998165 1104 30-31 0.27379349168229794 1104 32-33 0.2698263755390471 1104 34-35 0.17975434516012 1104 36-37 0.40558048358658993 1104 38-39 0.5093673515850625 1104 40-41 0.40484995011455993 1104 42-43 0.19336217222645757 1104 44-45 0.24606075430948238 1104 46-47 0.10188030122368019 1104 48-49 0.4439305722082807 1104 50-51 0.3862241069671555 1104 52-53 0.34298733825330174 1104 54-55 0.511837613840207 1104 56-57 0.4110690473760741 1104 58-59 0.24288660754675817 1104 60-61 0.4129816085930287 1104 62-63 0.3865715012345561 1104 64-65 0.40143421236211196 1104 66-67 0.1700627348947279 1104 68-69 0.162520163424567 1104 70-71 -0.0034806227090982134 1104 72-73 -0.03401057256761675 1104 74-75 -0.01330537448123792 1104 76-77 0.09902650982027694 1104 78-79 -0.13955028446826034 1104 80-81 -0.22484848918743694 1104 82-83 -0.04656340637355427 1104 84-85 -0.006242151835586185 1104 86-87 -0.085826301146124 1104 88-89 0.08311513928821412 1104 90-91 0.06441304989346008 1104 92-93 -0.1365056946173091 1104 94-95 -0.11591426543168026 1104 96-97 -0.23970847892461578 1104 98-99 -0.1617488988367377 1104 100-101 0.006232810671594535 1105 1 -0.04514563011528594 1105 2 -0.1291961257293721 1105 3 0.03599361844067772 1105 4 0.09381401489482499 1105 5 0.14752578873260802 1105 6 0.06514073798485498 1105 7 0.08805724402032666 1105 8 0.14041958817864497 1105 9 0.13237255230568934 1105 10-11 0.046091078978143685 1105 12-13 0.1837098998724045 1105 14-15 0.15311948060819702 1105 16-17 0.1356769067658803 1105 18-19 0.07693936987433148 1105 20-21 0.14231903345183383 1105 22-23 0.16115037987413672 1105 24-25 0.10097006336130931 1105 26-27 0.16659375529132348 1105 28-29 0.07872820445779638 1105 30-31 0.09857143467135643 1105 32-33 0.015138844969520449 1105 34-35 -0.0026900672202856413 1105 36-37 -0.05323564440432449 1105 38-39 0.14468273123407727 1105 40-41 0.13410486080265116 1105 42-43 -0.02529122808266493 1105 44-45 0.05270974771624992 1105 46-47 -0.061638519819886994 1105 48-49 -0.17800575025371046 1105 50-51 -0.11690254378704878 1105 52-53 -0.2727378686412223 1105 54-55 -0.09628739838760225 1105 56-57 -0.14119077400009417 1105 58-59 -0.1706267109827735 1105 60-61 0.07663615831619097 1105 62-63 0.16152907073742284 1105 64-65 0.14131386626620923 1105 66-67 0.05285429477186909 1105 68-69 -0.004509261574945356 1105 70-71 -0.023197353286448674 1105 72-73 0.08999591795338091 1105 74-75 0.17070908857121836 1105 76-77 0.08261556739858378 1105 78-79 0.062331453054479624 1105 80-81 -0.14147317370996504 1105 82-83 -0.13086681255202492 1105 84-85 -0.2450219143226242 1105 86-87 -0.3300657803392859 1105 88-89 -0.46376505753060115 1105 90-91 -0.5004267413533832 1105 92-93 -0.4427157096819663 1105 94-95 -0.419486742101018 1105 96-97 -0.2597223452599877 1105 98-99 -0.2599174176088681 1105 100-101 -0.2860093782040316 1106 1 0.19518504772518241 1106 2 0.11905894188164723 1106 3 0.24430990190421653 1106 4 0.2558735144587345 1106 5 0.1776496935463996 1106 6 0.17773535014805475 1106 7 0.22158286091384127 1106 8 0.25313228286867684 1106 9 0.2529161060519485 1106 10-11 0.31829293228924627 1106 12-13 0.3150574202562595 1106 14-15 0.3201238321246791 1106 16-17 0.31569430347874317 1106 18-19 0.29334372185847 1106 20-21 0.37598214049539536 1106 22-23 0.4598331166763643 1106 24-25 0.42970480324070337 1106 26-27 0.4231005701729913 1106 28-29 0.3928744911715327 1106 30-31 0.47049632015469456 1106 32-33 0.35813175767964367 1106 34-35 0.3797085045657056 1106 36-37 0.3102434175761388 1106 38-39 0.5753706262552001 1106 40-41 0.5817294144857712 1106 42-43 0.509313382513902 1106 44-45 0.49075617168630004 1106 46-47 0.5389853300446106 1106 48-49 0.5121462839224122 1106 50-51 0.4164394021662261 1106 52-53 0.4222508725990437 1106 54-55 0.2673091615527241 1106 56-57 0.30017131379251083 1106 58-59 0.26540295695981087 1106 60-61 0.3299155789869488 1106 62-63 0.3896253465782067 1106 64-65 0.20744651152525506 1106 66-67 0.06729602162943848 1106 68-69 0.2478985697517686 1106 70-71 0.2986681401932927 1106 72-73 0.36646686380611726 1106 74-75 0.19160268101580513 1106 76-77 0.2083892417760822 1106 78-79 0.30232132835465464 1106 80-81 0.29135131356635213 1106 82-83 0.04947443425916376 1106 84-85 0.289095751992523 1106 86-87 0.15505465214673464 1106 88-89 0.4259680759587745 1106 90-91 0.5311211685858908 1106 92-93 0.4202851765215492 1106 94-95 0.213946513212111 1106 96-97 0.3609702730622111 1106 98-99 0.31352231470622627 1106 100-101 0.5151495842597562 1107 1 0.00889274390068806 1107 2 0.09636274431898073 1107 3 0.11785382995739013 1107 4 0.13746580990295598 1107 5 0.15799685726600643 1107 6 0.07946565541042361 1107 7 0.13654296641703212 1107 8 0.10857041662660549 1107 9 0.25232176846819954 1107 10-11 0.1657642960238448 1107 12-13 0.2959035408527555 1107 14-15 0.31872289353442085 1107 16-17 0.35518632678471107 1107 18-19 0.29322297980759515 1107 20-21 0.27914756702909216 1107 22-23 0.32811125784422757 1107 24-25 0.3271209233520338 1107 26-27 0.36265676870600316 1107 28-29 0.17659129537754126 1107 30-31 0.23745644330055882 1107 32-33 0.2628756522117399 1107 34-35 0.33587748609824075 1107 36-37 0.4977458985771861 1107 38-39 0.5164760721076291 1107 40-41 0.28805773141876756 1107 42-43 0.36473607893199755 1107 44-45 0.2726596397949663 1107 46-47 0.49099394849091027 1107 48-49 0.45003249308513915 1107 50-51 0.3439754263014052 1107 52-53 0.3471460089548586 1107 54-55 0.2802125723226965 1107 56-57 0.3941979454090685 1107 58-59 0.332747800667768 1107 60-61 0.21798769782800065 1107 62-63 0.20899303313845508 1107 64-65 0.04396728797934202 1107 66-67 -0.09801583468861708 1107 68-69 -0.044979744314296966 1107 70-71 -0.04739346361399299 1107 72-73 -0.07929349134090913 1107 74-75 0.019794461293944465 1107 76-77 0.022023059253797328 1107 78-79 -0.013787976832734472 1107 80-81 -0.019749677510866093 1107 82-83 0.06093004308579353 1107 84-85 0.08630038054807443 1107 86-87 0.076371082328059 1107 88-89 0.25134640105151007 1107 90-91 0.2926567207795365 1107 92-93 0.04232175860629539 1107 94-95 0.12463819425020617 1107 96-97 0.36289167053842775 1107 98-99 0.3441488942768949 1107 100-101 0.2905032096087474 1108 1 0.03701687462675096 1108 2 0.062466103777424564 1108 3 0.13926424616840904 1108 4 0.04564446998419669 1108 5 0.14477062202109892 1108 6 0.1310328435175876 1108 7 0.1640677626220608 1108 8 0.21258919612883886 1108 9 0.18091920890103808 1108 10-11 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2316 64-65 -0.672121858012197 2316 66-67 -0.8064389554897318 2316 68-69 -0.6026405839183511 2316 70-71 -0.45084216664123034 2316 72-73 -0.5179753729021179 2316 74-75 -0.6055195547698524 2316 76-77 -0.6579945717398772 2316 78-79 -0.35848254579526895 2316 80-81 -0.30909387368735963 2316 82-83 -0.33992730719480235 2316 84-85 -0.4491157339496752 2316 86-87 -0.35388134049312825 2316 88-89 -0.46426163775972284 2316 90-91 -0.48395253952537587 2316 92-93 -0.5219852742011781 2316 94-95 -0.4995421950144774 2316 96-97 -0.49503046500127823 2316 98-99 -0.7551752509656176 2316 100-101 -0.710072966655094 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4139.0 3 757.0 4 735.0 5 792.0 6 894.0 7 1128.0 8 1291.0 9 1661.0 10 2076.0 11 3070.0 12 5030.0 13 9012.0 14 7965.0 15 9366.0 16 13239.0 17 19996.0 18 18256.0 19 8962.0 20 8631.0 21 8549.0 22 8770.0 23 9437.0 24 10217.0 25 11544.0 26 13111.0 27 15141.0 28 17674.0 29 21159.0 30 25130.0 31 30864.0 32 38831.0 33 51084.0 34 74691.0 35 131890.0 36 181222.0 37 276983.0 38 303851.0 39 62668.0 40 28.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.239257638751496 20.247690974124964 15.011052295219402 19.50199909190414 2 14.906006601410043 22.94203030285815 40.24907256438079 21.902890531351016 3 19.62336500362149 29.24403164188431 30.165523411869966 20.96707994262423 4 11.736179018539703 17.871885933983684 38.979183576947904 31.41275147052871 5 11.78442617012542 40.205522165227435 33.120774813449 14.889276851198147 6 27.501547583214542 38.205570163731466 18.291297755010476 16.001584498043513 7 25.795060278685533 36.186877059311726 21.689393492983164 16.328669169019573 8 25.65289904000784 33.172092138231484 20.66010199872342 20.514906823037258 9 27.573660955150302 17.794132680588607 22.064565502592615 32.56764086166847 10-11 24.26417319159343 28.082191537703306 26.482950001650536 21.170685269052733 12-13 26.8068273303355 26.537050946560136 27.542535926405005 19.113585796699358 14-15 23.139936126279277 26.88965081727054 28.80092832363253 21.169484732817654 16-17 19.4669029109225 30.514693746732384 28.932787160465434 21.085616181879683 18-19 19.230139811608428 28.53140125771875 33.18971744814084 19.04874148253198 20-21 21.160943179796977 26.60744189976522 32.84961588396745 19.381999036470344 22-23 20.176945401713215 26.98623308249854 33.153438604887974 19.68338291090027 24-25 20.445918046304115 27.30996797673928 33.232176097395026 19.011937879561575 26-27 19.812379021648 26.9845243586616 34.17818879494109 19.024907824749306 28-29 19.009745245885924 28.206210752260546 33.77419223114466 19.00985177070887 30-31 19.344306414029468 27.59605883695854 33.296550470454946 19.76308427855705 32-33 19.238307980639956 28.083106326522717 33.33693088685493 19.3416548059824 34-35 20.129503011999965 27.6253642027602 33.082330371085654 19.162802414154175 36-37 20.02452462601014 27.19738974163616 33.514316188070595 19.263769444283092 38-39 19.511559056464627 28.20352774779204 33.214482933068275 19.07043026267506 40-41 19.193038121215963 28.259694149779268 33.326803247566 19.220464481438764 42-43 19.13706073896082 28.482047960231128 32.02877343078874 20.352117870019313 44-45 19.490354985561698 27.972964883424602 32.06023038571506 20.476449745298645 46-47 20.236385996159182 28.508007168481775 31.693448693291536 19.562158142067506 48-49 20.023792832712083 28.631659079642198 31.34826883620046 19.996279251445266 50-51 19.255550816065377 29.065796596048266 31.644863230073028 20.033789357813333 52-53 19.7442807231612 29.691667224969386 30.38810829457287 20.17594375729654 54-55 19.21579987621606 28.944882652895792 30.84785585477728 20.99146161611087 56-57 20.025642066954365 28.99530691039099 30.26640247999185 20.712648542662798 58-59 20.53094319260448 28.510192705986693 30.564369783216893 20.394494318191935 60-61 20.030518381699792 29.12536639325206 31.336489367875735 19.507625857172407 62-63 19.6741344776012 29.153178599701725 31.225358967251697 19.947327955445378 64-65 19.427704080578035 29.566068385267467 30.91777901484216 20.08844851931234 66-67 19.86686326735662 29.08328690976577 30.099259111939432 20.950590710938176 68-69 19.377650084571005 29.086686616749873 30.772082478563167 20.763580820115955 70-71 20.333730759154605 29.027055252882228 30.289942631671806 20.349271356291357 72-73 19.947964837060443 28.713430596319405 30.844056675558978 20.49454789106118 74-75 19.152976735731166 30.27726788304777 30.08319105785236 20.486564323368704 76-77 19.501588960024062 29.04792822276657 30.331283376534707 21.11919944067467 78-79 19.42821230662459 28.96322063982135 30.6378761072036 20.97069094635046 80-81 19.854104577981012 29.464426634554236 30.00008906924421 20.681379718220537 82-83 19.812523183392976 28.835918411560325 30.408950598038004 20.9426078070087 84-85 19.34144897047285 28.998187735752918 30.21197006592708 21.448393227847156 86-87 19.508072062390024 29.1798659181726 30.116243684848556 21.19581833458882 88-89 19.436015077706784 28.819845495201886 29.878007709915583 21.866131717175747 90-91 19.67285337428102 28.230627285886346 30.137681601094407 21.95883773873823 92-93 20.237768952247688 28.63994605362062 30.068085206481403 21.054199787650283 94-95 19.806980756886073 28.161013563100155 30.346336130925454 21.685669549088317 96-97 19.340281527690305 28.059919711179802 30.825368073592262 21.774430687537624 98-99 19.74400070710948 28.4191006522586 29.832785002721156 22.004113637910763 100-101 19.749721683327657 27.355107841474247 30.65670618454569 22.238464290652406 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10289.0 1 6392.0 2 3657.5 3 4588.0 4 3507.5 5 3162.0 6 5332.0 7 9997.5 8 14747.0 9 14813.5 10 10228.0 11 6740.0 12 6138.0 13 6091.0 14 5941.5 15 5598.5 16 5432.0 17 5393.5 18 5237.0 19 5207.0 20 5324.5 21 5725.0 22 6336.0 23 7015.5 24 7573.5 25 8388.5 26 9797.5 27 11536.0 28 12673.5 29 14249.5 30 17190.0 31 20189.0 32 23980.5 33 28375.5 34 31944.5 35 34563.0 36 39303.5 37 44045.5 38 44638.5 39 44162.0 40 42883.5 41 41284.0 42 39779.5 43 40772.0 44 42885.0 45 43040.5 46 42071.0 47 47282.5 48 66731.5 49 65722.5 50 46010.0 51 37398.5 52 33541.5 53 32442.5 54 31318.0 55 29068.0 56 26287.5 57 23706.0 58 21458.0 59 18950.0 60 16670.0 61 14702.0 62 12801.0 63 9986.5 64 7174.0 65 5570.5 66 4221.0 67 3477.5 68 2727.5 69 1859.5 70 1539.5 71 1281.0 72 1104.0 73 980.0 74 758.0 75 632.5 76 476.5 77 278.0 78 180.0 79 127.5 80 78.5 81 48.0 82 42.0 83 40.0 84 25.5 85 14.5 86 10.0 87 9.0 88 8.5 89 6.5 90 5.0 91 3.5 92 2.0 93 0.5 94 1.0 95 1.5 96 4.0 97 3.5 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3337957958469164 2 0.11774352339691484 3 0.11235285606067055 4 0.11894933056423264 5 0.1360434204067968 6 0.08483208071247599 7 0.09242157288324099 8 0.09894711755343145 9 0.08710183538036832 10-11 0.0856477737962498 12-13 0.090080888381977 14-15 0.09103844113249408 16-17 0.08032803629337715 18-19 0.09515237146804895 20-21 0.10501161830670629 22-23 0.11440982122844798 24-25 0.12802834923580197 26-27 0.12714172631865653 28-29 0.12210570814927041 30-31 0.1120336718104982 32-33 0.10494068847333464 34-35 0.32262434709088383 36-37 0.479804857842428 38-39 0.4538800037450952 40-41 0.4316080360664017 42-43 0.4635619260003235 44-45 0.4704066549206863 46-47 0.4793083490088265 48-49 0.48945131518097035 50-51 0.4780316120081371 52-53 0.4810461299264316 54-55 0.4676049265025067 56-57 0.44621958174095855 58-59 0.42710399164730284 60-61 0.3861774777918692 62-63 0.45700091641344714 64-65 0.48806818343022346 66-67 0.49654429851813386 68-69 0.5090279491915418 70-71 0.5012256675206619 72-73 0.4927140875160656 74-75 0.4999843954366583 76-77 0.4770031294242484 78-79 0.4554404600792712 80-81 0.45689452166338973 82-83 0.47030026017062887 84-85 0.4700520057538281 86-87 0.47317291842218007 88-89 0.48555017434553044 90-91 0.5020768255211215 92-93 0.4943454736836132 94-95 0.498672193519283 96-97 0.4988849830193979 98-99 0.49292687701618054 100-101 0.47991125259248546 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1409844.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.59499691079435 #Duplication Level Percentage of deduplicated Percentage of total 1 70.0855481844201 25.64780419300182 2 14.191692808156347 10.386899089468479 3 5.700986858829184 6.258827894619995 4 2.9402304866918074 4.3039090231004025 5 1.725526871289395 3.157282526216402 6 1.0813601430376634 2.374342265635166 7 0.7270680421909181 1.8624936930539762 8 0.5265897187837961 1.5416439305719265 9 0.3760718522754212 1.2386113445030174 >10 2.2306408374562166 15.63699387618373 >50 0.2552047324196128 6.524348981272683 >100 0.14588751215211587 9.196214183243363 >500 0.006595976148727446 1.5559763152872128 >1k 0.005237981059283561 4.401417329874588 >5k 5.819978954759512E-4 1.5010269304246722 >10k+ 7.759971939679349E-4 4.412208423542683 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22603 1.6032270236990758 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15330 1.0873543455871713 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12287 0.8715148626372846 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11929 0.8461219822902393 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9507 0.6743299258641381 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6429 0.4560078987462443 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5207 0.3693316423661058 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4511 0.31996447833944747 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4455 0.3159924076706359 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4188 0.29705414216040926 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4146 0.29407508915880054 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3345 0.23726029262812054 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3041 0.2156976232831434 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2918 0.20697325377843223 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2876 0.2039942007768235 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2692 0.19094311143644263 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2666 0.1890989357687801 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2509 0.17796295192943332 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2439 0.17299786359341884 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2244 0.15916654608594993 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2186 0.15505261575039508 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1948 0.13817131540794586 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1828 0.1296597354033496 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1583 0.1122819262272989 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1538 0.10909008372557531 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1481 0.10504708322339208 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.4185966674327088E-4 0.0 10-11 0.0 0.0 0.0 1.4185966674327088E-4 0.0 12-13 0.0 0.0 0.0 1.4185966674327088E-4 0.0 14-15 0.0 0.0 0.0 1.4185966674327088E-4 0.0 16-17 0.0 0.0 0.0 2.8371933348654175E-4 0.0 18-19 0.0 0.0 0.0 2.8371933348654175E-4 0.0 20-21 0.0 0.0 0.0 6.38368500344719E-4 0.0 22-23 0.0 0.0 0.0 0.0017377809176050683 0.0 24-25 0.0 0.0 0.0 0.0030145179182945064 0.0 26-27 0.0 0.0 0.0 0.004752298835899575 0.0 28-29 0.0 0.0 0.0 0.010213896005515505 0.0 30-31 0.0 0.0 0.0 0.023796959096183692 0.0 32-33 0.0 0.0 0.0 0.04755845327568156 0.0 34-35 0.0 0.0 0.0 0.0812146592105226 0.0 36-37 0.0 0.0 0.0 0.1348730781561648 0.0 38-39 0.0 0.0 0.0 0.2147400705326263 0.0 40-41 0.0 0.0 0.0 0.3101761613341618 0.0 42-43 0.0 0.0 0.0 0.4038390063014064 0.0 44-45 0.0 0.0 0.0 0.514241291944357 0.0 46-47 0.0 0.0 0.0 0.6462417118489705 0.0 48-49 0.0 0.0 0.0 0.7862217380078931 0.0 50-51 0.0 0.0 0.0 0.9495376793460837 0.0 52-53 0.0 0.0 0.0 1.1110449099332977 0.0 54-55 0.0 0.0 0.0 1.2900008795299338 0.0 56-57 0.0 0.0 0.0 1.5009107390604917 0.0 58-59 0.0 0.0 0.0 1.7354047681871185 0.0 60-61 0.0 0.0 0.0 2.006108477249965 0.0 62-63 0.0 0.0 0.0 2.273939528061261 0.0 64-65 0.0 0.0 0.0 2.5473031058755433 0.0 66-67 0.0 0.0 0.0 2.8616286624619462 0.0 68-69 0.0 0.0 0.0 3.220356294738992 0.0 70-71 0.0 0.0 0.0 3.6151162823688296 0.0 72-73 0.0 0.0 0.0 4.0085286031646055 0.0 74-75 0.0 0.0 0.0 4.424141961805703 0.0 76-77 0.0 0.0 0.0 4.8606441563747484 0.0 78-79 0.0 0.0 0.0 5.373786035901844 0.0 80-81 0.0 0.0 0.0 5.951190344463642 0.0 82-83 0.0 0.0 0.0 6.513876712600827 0.0 84-85 0.0 0.0 0.0 7.090323468412108 0.0 86-87 0.0 0.0 0.0 7.675636453394844 0.0 88-89 0.0 0.0 0.0 8.31021020765418 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAGG 530 0.0 26.869171 1 GTACATG 12545 0.0 25.654755 1 TACATGG 12770 0.0 25.116411 2 TAGTACA 155 1.4434536E-6 24.452208 4 ACATGGG 12545 0.0 24.282875 3 GAGTACT 11690 0.0 23.800364 12-13 CATGGGG 3085 0.0 23.649683 4 ATGGGAG 2710 0.0 23.076208 5 AGTACTT 12185 0.0 22.580877 12-13 CATGGGA 8910 0.0 22.491709 4 AACTCGA 195 0.0 21.94345 36-37 GTACTTT 12785 0.0 21.763084 14-15 CATGGGT 860 0.0 21.484535 4 TCGCGCT 180 0.0 21.13525 60-61 GTATCAA 26905 0.0 20.85419 1 CGCGCTT 200 0.0 20.211664 62-63 ACTTTTT 14075 0.0 19.954586 16-17 GAGTGCA 500 0.0 19.88951 9 TACTTTT 13835 0.0 19.786182 14-15 AGAGTAC 20190 0.0 19.714064 10-11 >>END_MODULE