##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939079_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1409844 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.38372543345221 34.0 31.0 34.0 31.0 34.0 2 32.56804795424175 34.0 31.0 34.0 31.0 34.0 3 32.63942393626529 34.0 31.0 34.0 31.0 34.0 4 36.14967471578416 37.0 35.0 37.0 35.0 37.0 5 36.12722329562703 37.0 35.0 37.0 35.0 37.0 6 36.19793395581355 37.0 37.0 37.0 35.0 37.0 7 36.144631604631435 37.0 36.0 37.0 35.0 37.0 8 36.1456154014203 37.0 36.0 37.0 35.0 37.0 9 37.838677896277886 39.0 38.0 39.0 35.0 39.0 10-11 37.78342391073055 39.0 38.0 39.0 35.0 39.0 12-13 37.71205608563784 39.0 38.0 39.0 35.0 39.0 14-15 38.929983388233026 40.0 38.0 41.0 35.0 41.0 16-17 38.91169448534731 40.0 38.0 41.0 35.5 41.0 18-19 39.020516454302744 40.0 38.5 41.0 35.0 41.0 20-21 39.09822540649888 41.0 39.0 41.0 35.0 41.0 22-23 39.02150592547828 40.0 38.5 41.0 35.0 41.0 24-25 38.92789663253523 40.0 38.0 41.0 35.0 41.0 26-27 38.737268449558954 40.0 38.0 41.0 35.0 41.0 28-29 38.55069000541904 40.0 38.0 41.0 34.0 41.0 30-31 38.249687908733165 40.0 37.5 41.0 34.0 41.0 32-33 37.935776582373656 40.0 36.5 41.0 33.5 41.0 34-35 37.76168178890714 40.0 36.0 41.0 33.0 41.0 36-37 37.58531724077274 40.0 35.5 41.0 33.0 41.0 38-39 37.39694285325185 40.0 35.0 41.0 33.0 41.0 40-41 37.18174067485481 40.0 35.0 41.0 33.0 41.0 42-43 36.912991082701346 40.0 35.0 41.0 32.0 41.0 44-45 36.53657603252559 40.0 35.0 41.0 31.0 41.0 46-47 36.024655210079985 39.5 35.0 41.0 29.5 41.0 48-49 35.53591106533773 39.0 35.0 41.0 27.5 41.0 50-51 35.23420676330147 39.0 35.0 41.0 26.5 41.0 52-53 35.010790910199994 39.0 35.0 41.0 26.0 41.0 54-55 34.771973707729366 38.5 35.0 40.0 25.0 41.0 56-57 34.48257821432726 38.0 34.5 40.0 23.5 41.0 58-59 34.191511968700084 38.0 34.0 40.0 23.0 41.0 60-61 33.79972926082603 37.0 34.0 40.0 22.0 41.0 62-63 33.427674976805946 37.0 33.5 40.0 20.5 41.0 64-65 33.02843896204119 36.0 33.0 39.5 19.5 41.0 66-67 32.6558605065525 36.0 33.0 39.0 17.5 40.5 68-69 32.17910102110588 35.0 32.5 38.5 12.5 40.0 70-71 31.74572718683769 35.0 32.0 38.0 7.5 40.0 72-73 31.28083107067165 35.0 31.0 37.0 4.5 39.5 74-75 30.801804667750474 35.0 31.0 37.0 2.0 39.0 76-77 28.864749575130297 33.0 28.5 35.0 2.0 37.0 78-79 29.821154680943422 34.0 30.0 36.0 2.0 38.0 80-81 29.82274563710595 34.0 30.0 35.0 2.0 37.0 82-83 29.51189564235476 34.0 30.0 35.0 2.0 37.0 84-85 29.11157085464775 34.0 30.0 35.0 2.0 36.0 86-87 28.574259279750102 34.0 29.0 35.0 2.0 36.0 88-89 28.003940152243793 34.0 28.5 35.0 2.0 35.5 90-91 27.520425309466862 34.0 27.0 35.0 2.0 35.0 92-93 27.224240057765257 33.0 26.5 35.0 2.0 35.0 94-95 26.871840075923295 33.0 26.0 35.0 2.0 35.0 96-97 26.29322038466667 33.0 25.0 35.0 2.0 35.0 98-99 25.549001166086462 33.0 22.0 35.0 2.0 35.0 100-101 22.099199982409402 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.196624191559728 1101 2 0.23688283468857918 1101 3 0.28716682475356947 1101 4 0.1506436601202097 1101 5 0.14019819018244561 1101 6 0.24404855393301972 1101 7 0.23987709308363492 1101 8 0.1856577329800757 1101 9 0.3092173384887573 1101 10-11 0.3598104574490364 1101 12-13 0.36422964638678224 1101 14-15 0.44126256473553127 1101 16-17 0.3699615424279017 1101 18-19 0.33854780420515596 1101 20-21 0.3450524284267118 1101 22-23 0.3923012138673343 1101 24-25 0.33711107656900197 1101 26-27 0.2994171209544092 1101 28-29 0.30057208260257795 1101 30-31 0.4341227030725179 1101 32-33 0.3688578213186986 1101 34-35 0.5129058626311078 1101 36-37 0.6661274323767472 1101 38-39 0.8078560483240551 1101 40-41 0.6324872737397271 1101 42-43 0.5319359219660669 1101 44-45 0.5023365087760041 1101 46-47 0.41883552189397477 1101 48-49 0.42096183825095324 1101 50-51 0.624353150737015 1101 52-53 0.6179978076295427 1101 54-55 0.5194907788310488 1101 56-57 0.5388015658970957 1101 58-59 0.5539688749310585 1101 60-61 0.6606595815899396 1101 62-63 0.6372080366897919 1101 64-65 0.3786963295553534 1101 66-67 0.5025760154086925 1101 68-69 0.606493488479579 1101 70-71 0.44245771943578305 1101 72-73 0.3631278779330458 1101 74-75 0.43795488621347545 1101 76-77 0.4297023728233249 1101 78-79 0.45736061795668803 1101 80-81 0.40458200628951957 1101 82-83 0.4610539001642451 1101 84-85 0.5696131609361679 1101 86-87 0.43683894335162776 1101 88-89 0.7818345712496324 1101 90-91 0.9355323561649307 1101 92-93 0.6473400411810708 1101 94-95 0.7062276631153033 1101 96-97 0.6806220444405184 1101 98-99 0.44247198450103653 1101 100-101 0.2538981368081856 1102 1 0.22908187349342768 1102 2 0.18412450204395014 1102 3 0.27089188009507836 1102 4 0.21585477063177905 1102 5 0.1123993716663847 1102 6 0.19229357422205595 1102 7 0.25024735012367216 1102 8 0.23831839410018318 1102 9 0.28442038256184077 1102 10-11 0.27323455472167524 1102 12-13 0.26932741694886175 1102 14-15 0.3897678147507335 1102 16-17 0.36260066117120004 1102 18-19 0.3460572418015957 1102 20-21 0.3323541057131649 1102 22-23 0.29609407920151654 1102 24-25 0.27330527488044964 1102 26-27 0.2219195473746325 1102 28-29 0.12752921846367116 1102 30-31 0.27229054919683904 1102 32-33 0.32424515721516656 1102 34-35 0.5038510975925945 1102 36-37 0.5129231575058597 1102 38-39 0.6165486091006755 1102 40-41 0.5403280432000912 1102 42-43 0.5483522512789349 1102 44-45 0.4862179751602653 1102 46-47 0.5434001835167805 1102 48-49 0.5743211581292869 1102 50-51 0.5666753834397653 1102 52-53 0.4803355994744436 1102 54-55 0.4325238484968068 1102 56-57 0.5050936201478251 1102 58-59 0.3786717110252624 1102 60-61 0.4863128367496259 1102 62-63 0.5023386877217391 1102 64-65 0.3492329970165997 1102 66-67 0.37875447699338594 1102 68-69 0.44456127251758915 1102 70-71 0.5105334059880455 1102 72-73 0.44423998093757433 1102 74-75 0.5415826661807053 1102 76-77 0.700835792808828 1102 78-79 0.701393263468475 1102 80-81 0.533101681637568 1102 82-83 0.6963244537022426 1102 84-85 0.6534815816832413 1102 86-87 0.6995220689223736 1102 88-89 0.6387365670955205 1102 90-91 0.9128551455270681 1102 92-93 0.89007290409738 1102 94-95 0.6951398960297901 1102 96-97 0.7524498884748034 1102 98-99 0.5620782794649415 1102 100-101 0.19567702896721073 1103 1 0.08535685361587753 1103 2 0.05690550802786021 1103 3 0.07257517908941225 1103 4 0.06759753580231376 1103 5 0.05789892084218451 1103 6 0.045860279556166006 1103 7 0.1538448873050129 1103 8 0.1735681853359523 1103 9 0.13751029302295592 1103 10-11 0.145955628389153 1103 12-13 0.16214179857387734 1103 14-15 0.10868891319022822 1103 16-17 0.12402842208654619 1103 18-19 0.14238159283448937 1103 20-21 0.10434227374206984 1103 22-23 0.1310361512881073 1103 24-25 0.09413994559232464 1103 26-27 0.13293337575777997 1103 28-29 0.060341762164313195 1103 30-31 0.018751878868528138 1103 32-33 0.01633089505523344 1103 34-35 -0.004257078906739764 1103 36-37 -6.658293316732511E-4 1103 38-39 0.02834155759751411 1103 40-41 -0.17431988107742313 1103 42-43 -0.058087875671034794 1103 44-45 -0.016525260145989762 1103 46-47 -0.020582225682431954 1103 48-49 -0.18636577276341626 1103 50-51 -0.36855975767419835 1103 52-53 -0.3792439819848923 1103 54-55 -0.42177436405515323 1103 56-57 -0.3404418218263743 1103 58-59 -0.32240420723771024 1103 60-61 -0.42085100621704896 1103 62-63 -0.30348826503961135 1103 64-65 -0.36092533084513434 1103 66-67 -0.24675910966274728 1103 68-69 -0.42132518637482974 1103 70-71 -0.2222097494084565 1103 72-73 -0.3810080389652839 1103 74-75 -0.4459687257806415 1103 76-77 -0.21603941612188393 1103 78-79 -0.15836911155599154 1103 80-81 -0.19056805400764532 1103 82-83 -0.3011460695867889 1103 84-85 -0.3153216498672613 1103 86-87 -0.23108330074681405 1103 88-89 -0.3221522691121237 1103 90-91 -0.11861831044998183 1103 92-93 -0.1347703728951366 1103 94-95 -0.21574774153152632 1103 96-97 -0.23288866918075257 1103 98-99 -0.30927067711342815 1103 100-101 -0.2970292081223711 1104 1 -0.054541870336592524 1104 2 -0.03721577783541363 1104 3 0.007464036167995403 1104 4 0.034314729724727044 1104 5 0.054486413940480816 1104 6 0.10844846793677476 1104 7 0.19399232805265143 1104 8 0.0969197016648593 1104 9 0.11281882454583325 1104 10-11 0.1156194645070201 1104 12-13 0.06054622793114817 1104 14-15 0.14822478668974526 1104 16-17 0.15412047030641673 1104 18-19 0.08600429482422811 1104 20-21 0.10092337637801307 1104 22-23 0.10460903323326676 1104 24-25 0.15632297865912648 1104 26-27 0.11880534156625089 1104 28-29 0.036482331886816155 1104 30-31 0.028368885678204947 1104 32-33 0.04441633809321388 1104 34-35 0.10638483025666545 1104 36-37 0.06569273184293678 1104 38-39 0.12646263385362033 1104 40-41 0.3067381494332011 1104 42-43 0.235000455855932 1104 44-45 0.29526220330055963 1104 46-47 0.30326659642754805 1104 48-49 0.23364864411966835 1104 50-51 0.1635938537038868 1104 52-53 0.16360662434217943 1104 54-55 0.4258092743162507 1104 56-57 0.6324063816868417 1104 58-59 0.4032276816807112 1104 60-61 0.45628085202714885 1104 62-63 0.4301930502709084 1104 64-65 0.5772558924427358 1104 66-67 0.4160590075536703 1104 68-69 0.24314230917740787 1104 70-71 0.5436999456897986 1104 72-73 0.26775233914559493 1104 74-75 0.2764871033435057 1104 76-77 0.26744443390163397 1104 78-79 0.33917247757306157 1104 80-81 0.14923903071455058 1104 82-83 0.12829300118886167 1104 84-85 0.12733289707452045 1104 86-87 0.2181118844232266 1104 88-89 0.38765747404241324 1104 90-91 0.4093687194676079 1104 92-93 0.31002370084568653 1104 94-95 0.1338226915884526 1104 96-97 0.26326299438441225 1104 98-99 0.14305200033692955 1104 100-101 -0.28717064183538454 1105 1 0.018403124087519984 1105 2 -0.0045699734412281146 1105 3 0.0775440549499109 1105 4 0.06367736740985919 1105 5 0.0977410653990205 1105 6 0.09235422576746544 1105 7 -0.01754387604415797 1105 8 0.04672576861455724 1105 9 0.07456412417606373 1105 10-11 0.15908382288942136 1105 12-13 0.06404582685901516 1105 14-15 0.1180667300309679 1105 16-17 0.21388205248707948 1105 18-19 0.09962179428620033 1105 20-21 0.11623137043040543 1105 22-23 0.1437392447254453 1105 24-25 0.12845702479220478 1105 26-27 0.177457250082675 1105 28-29 0.20218381683194053 1105 30-31 0.0470401385884216 1105 32-33 0.15698004497795637 1105 34-35 0.21517809224481255 1105 36-37 0.19848184615828046 1105 38-39 0.32210574323870844 1105 40-41 0.2042085825911073 1105 42-43 0.11133206425301267 1105 44-45 0.183556888605672 1105 46-47 0.20160642680338015 1105 48-49 0.2809776015277592 1105 50-51 0.190647269664602 1105 52-53 0.13169815682901742 1105 54-55 0.22786183433033358 1105 56-57 0.27325117655312425 1105 58-59 0.34514803462418797 1105 60-61 0.3123228569965377 1105 62-63 0.29010637956091756 1105 64-65 0.12582474968061774 1105 66-67 0.13717019746621872 1105 68-69 -0.031755955451941986 1105 70-71 0.17379405090765587 1105 72-73 0.300060044621695 1105 74-75 0.1770640113708204 1105 76-77 0.11079213898644369 1105 78-79 0.30370388347762756 1105 80-81 0.128089200925654 1105 82-83 0.3283693027168475 1105 84-85 0.4066454007428639 1105 86-87 0.5436412262736319 1105 88-89 0.5804209344145264 1105 90-91 0.7318166534570025 1105 92-93 0.4852897779689265 1105 94-95 0.43670124009861766 1105 96-97 0.18224030935320457 1105 98-99 0.3145317364119009 1105 100-101 0.11245922238702732 1106 1 0.09257531880911785 1106 2 0.04266654469086717 1106 3 0.10180709137778621 1106 4 0.022507485235173874 1106 5 0.034323733690001745 1106 6 0.013897155196197275 1106 7 0.05500556078403207 1106 8 0.04217549936301168 1106 9 0.1317020375525928 1106 10-11 0.007326146940620504 1106 12-13 0.05120815175198601 1106 14-15 -0.05095379294602509 1106 16-17 0.052285529959036126 1106 18-19 0.008117773634616299 1106 20-21 0.12406749058931155 1106 22-23 0.03773562508680328 1106 24-25 -0.04174549258696203 1106 26-27 0.02848620485355724 1106 28-29 0.08497521761805871 1106 30-31 0.08846255369175537 1106 32-33 0.11126281638654945 1106 34-35 -0.07976867708740087 1106 36-37 0.08087398770766896 1106 38-39 0.22175780898145092 1106 40-41 -0.0035757449347499914 1106 42-43 0.12711946426283305 1106 44-45 0.33132606415581023 1106 46-47 0.3725858336783574 1106 48-49 0.3827442798078309 1106 50-51 0.40709897157285724 1106 52-53 0.3673435304491477 1106 54-55 0.4399456518799738 1106 56-57 0.5146475199698486 1106 58-59 0.4595314216987134 1106 60-61 0.3227877747947332 1106 62-63 0.5457304983262148 1106 64-65 0.5519202866287571 1106 66-67 0.5710977626045164 1106 68-69 0.24199090834629544 1106 70-71 0.38966118585142695 1106 72-73 0.3334043288428319 1106 74-75 0.2261617907178355 1106 76-77 0.2704432226566844 1106 78-79 0.40583942351227975 1106 80-81 0.2893969473732376 1106 82-83 0.17040411017218204 1106 84-85 0.35267233291205 1106 86-87 0.5173965078361569 1106 88-89 0.5153247263835112 1106 90-91 0.641929545029182 1106 92-93 0.5541757336306894 1106 94-95 0.34563915207218443 1106 96-97 0.5284693847787167 1106 98-99 0.7208355896435066 1106 100-101 0.5736720351595785 1107 1 0.011643962043372369 1107 2 -0.009025387755329461 1107 3 0.0693159016881637 1107 4 0.055730845899951476 1107 5 0.107910224694443 1107 6 0.06663110808143813 1107 7 0.09733033595020402 1107 8 0.09427872664917203 1107 9 0.21179426463145035 1107 10-11 0.20204393031504253 1107 12-13 0.18460055128797848 1107 14-15 0.23372894766697527 1107 16-17 0.13888239044517547 1107 18-19 0.1517737988283514 1107 20-21 0.18846766172325147 1107 22-23 0.21429137814229193 1107 24-25 0.18138802275995403 1107 26-27 0.15993074290218345 1107 28-29 0.16352591604742628 1107 30-31 0.23491273739610108 1107 32-33 0.22223468514269484 1107 34-35 0.05879192329279448 1107 36-37 0.1038011940343253 1107 38-39 0.23913308630223185 1107 40-41 0.19812026737984212 1107 42-43 0.15009022594117738 1107 44-45 -0.19276114799293964 1107 46-47 -0.32849268504271834 1107 48-49 -0.21556621361366268 1107 50-51 -0.35941953338693367 1107 52-53 -0.3211910469867547 1107 54-55 -0.30248398693037615 1107 56-57 -0.2736348774931372 1107 58-59 -0.4201383736021569 1107 60-61 -0.3965718622954597 1107 62-63 -0.5540487477141056 1107 64-65 -0.5752563782883442 1107 66-67 -0.46599320302314595 1107 68-69 -0.5545151805816069 1107 70-71 -0.44068024399909334 1107 72-73 -0.3854530433690755 1107 74-75 -0.3925745086430581 1107 76-77 -0.39840764381914795 1107 78-79 -0.13898123870549028 1107 80-81 -0.32840263625964994 1107 82-83 -0.26905725898754085 1107 84-85 -0.056377476767604406 1107 86-87 -0.1604989857838106 1107 88-89 -0.10529492843756572 1107 90-91 -0.0762789075759791 1107 92-93 -0.053854219219264365 1107 94-95 0.07312985658827031 1107 96-97 -0.013439684878974845 1107 98-99 0.2985339263804043 1107 100-101 0.37626650065271505 1108 1 0.06232143831949344 1108 2 0.026235548374401674 1108 3 0.06367929057716992 1108 4 0.04537281651609959 1108 5 0.09607164262754964 1108 6 0.11522126519308529 1108 7 0.06873533744720817 1108 8 0.10209927240988037 1108 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44-45 -0.48686023878679663 2316 46-47 -0.4153757443082142 2316 48-49 -0.34649362692794483 2316 50-51 -0.222927697804586 2316 52-53 -0.19059637546483543 2316 54-55 -0.06557039032291101 2316 56-57 -0.2460360344550736 2316 58-59 -0.040742148709043136 2316 60-61 0.09213076161124434 2316 62-63 0.04964569448965506 2316 64-65 0.017618914121108276 2316 66-67 -0.03688160920436445 2316 68-69 0.02951148485588817 2316 70-71 0.2522408544233379 2316 72-73 0.22284792806410536 2316 74-75 0.0827017678754558 2316 76-77 -0.020791803581300172 2316 78-79 0.1443116662599948 2316 80-81 0.3123010478113777 2316 82-83 0.22278120423512604 2316 84-85 0.011084121084412146 2316 86-87 0.20118414031813003 2316 88-89 -0.15482304007748127 2316 90-91 -0.3203687795496144 2316 92-93 -0.3750278326510852 2316 94-95 0.04996036857849262 2316 96-97 -0.17993445896026117 2316 98-99 -0.05936372166808468 2316 100-101 0.1943345240224268 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 18.0 5 56.0 6 147.0 7 316.0 8 601.0 9 1091.0 10 1794.0 11 2829.0 12 4460.0 13 6558.0 14 7611.0 15 10372.0 16 14348.0 17 21789.0 18 20246.0 19 11242.0 20 9534.0 21 9600.0 22 9937.0 23 10700.0 24 11733.0 25 13591.0 26 15312.0 27 18242.0 28 21564.0 29 25424.0 30 31422.0 31 37831.0 32 46193.0 33 59535.0 34 84369.0 35 139264.0 36 184519.0 37 291984.0 38 261540.0 39 24065.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.334022059645164 20.80684180670729 15.491715711429608 19.367420422217936 2 15.175579709528147 23.471958599674856 39.52990543634615 21.82255625445085 3 19.570179395734563 29.146983637906036 30.526356107484233 20.756480858875168 4 11.808185870209753 18.44636711579437 38.53454708464199 31.210899929353886 5 11.630151988446949 40.49483488953388 33.03443501550526 14.840578106513913 6 27.345578659766613 38.67491722488445 17.8496344276388 16.12986968771013 7 25.60418032065959 36.424171752335724 21.417334116398692 16.554313810605997 8 25.704971613880684 33.569458748627504 20.291748590624213 20.4338210468676 9 27.042211762436132 17.902831802667528 21.915190616834206 33.13976581806214 10-11 24.24672516959323 28.362180496565575 26.252372602926282 21.13872173091491 12-13 26.73132630276825 26.72029671367896 27.42448809939256 19.123888884160234 14-15 22.89415138497244 27.17278933374568 28.773869464357215 21.15918981692467 16-17 19.253336246238874 30.715702310511002 28.949765146990604 21.081196296259517 18-19 19.013883455858753 28.51887461267864 33.38154199384542 19.08569993761719 20-21 20.909547439291156 26.560917378092896 33.176542936665335 19.352992245950613 22-23 20.012675161223513 26.9342565560445 33.46373783198708 19.589330450744903 24-25 20.278605984152108 27.297059490062882 33.591636784444766 18.832697741340244 26-27 19.634431620221417 26.96824869913578 34.4953157539338 18.902003926709 28-29 18.80768368698948 28.17747921046584 34.062350160727 18.952486941817675 30-31 19.26716685493181 27.465974843994108 33.61712012852864 19.649738172545444 32-33 19.151338729675057 27.927770732080997 33.62829504540928 19.292595492834668 34-35 19.927048282275763 27.519162771924087 33.4903644358248 19.063424509975352 36-37 19.856760694436414 27.173856361250536 33.8387161887234 19.130666755589644 38-39 19.33172454965349 28.108014346386994 33.59890206366768 18.961359040291835 40-41 19.050904233471215 28.192483650162064 33.524385892354616 19.232226224012113 42-43 19.052322853203822 28.395519754387372 32.34029663768768 20.21186075472113 44-45 19.363883657668968 27.843637949929246 32.337167196881836 20.455311195519947 46-47 20.138420698912814 28.523991325210616 31.81225429261987 19.525333683256697 48-49 19.934701717502296 28.588036355950113 31.466771359546275 20.010490567001316 50-51 19.205394073881248 29.03393178037137 31.685467216751363 20.07520692899602 52-53 19.753398160859696 29.69070680540074 30.390146140334018 20.165748893405542 54-55 19.146373641339043 28.926994759703913 30.786668595958133 21.13996300299891 56-57 19.97079109461756 28.96040271122195 30.262851776508608 20.805954417651883 58-59 20.47934934805708 28.528620655900966 30.529385310962088 20.462644685079866 60-61 19.92690653769378 29.25572213134407 31.251037453305734 19.566333877656415 62-63 19.553124141049626 29.215644201697067 31.158844861370948 20.07238679588236 64-65 19.27874454850426 29.60527925738011 30.792842629854388 20.32313356426124 66-67 19.749920733043787 29.128779898892958 30.060157767842515 21.061141600220743 68-69 19.29984345286101 29.096594620935324 30.621634715303387 20.981927210900277 70-71 20.203128928849196 29.079220495040765 30.185548963413318 20.532101612696728 72-73 19.82510121687222 28.732505156598886 30.66654892314327 20.775844703385623 74-75 18.996215184091287 30.29895506169477 29.842131469864754 20.86269828434919 76-77 19.30514719340438 29.00058178649324 30.29597188375501 21.39829913634737 78-79 19.241891621590565 28.974109082036374 30.543154603578564 21.240844692794493 80-81 19.664469899609802 29.389827911638466 29.89440338658206 21.051298802169672 82-83 19.589612714643305 28.829178367175256 30.286366031866052 21.294842886315386 84-85 19.062357253710342 28.92841335637134 30.11460842476189 21.894620965156427 86-87 19.143570924197732 29.13810725617006 29.94352789648071 21.774793923151503 88-89 19.088790353992195 28.775350641897017 29.72165830101121 22.414200703099578 90-91 19.29047313064358 28.191046455124585 30.00211815005402 22.51636226417781 92-93 19.72760816090291 28.66505088506246 29.847344812617564 21.759996141417066 94-95 19.232659783635636 28.09403026150411 30.17074938787554 22.502560566984716 96-97 18.722603351860208 28.035548613889194 30.606010310360578 22.635837723890017 98-99 19.061019876545178 28.450111804458345 29.551239424681384 22.937628894315086 100-101 18.930229424010612 27.31560785481989 30.52984923767675 23.224313483492747 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8848.0 1 5664.0 2 3355.0 3 4239.0 4 3458.5 5 3086.0 6 5266.5 7 10090.5 8 14411.0 9 14278.5 10 10155.0 11 6658.0 12 5916.5 13 5827.0 14 5690.0 15 5451.0 16 5184.0 17 5121.5 18 5066.5 19 5138.0 20 5434.5 21 5818.0 22 6241.0 23 6946.5 24 7585.0 25 8402.5 26 9860.0 27 11516.0 28 12747.5 29 14490.0 30 17324.0 31 20204.0 32 23974.0 33 28526.5 34 32201.0 35 34825.0 36 39297.5 37 43825.0 38 44769.5 39 44472.0 40 43210.5 41 41746.5 42 40110.5 43 41063.5 44 43125.5 45 43196.0 46 41985.5 47 47917.5 48 67612.5 49 65504.0 50 45324.0 51 37381.0 52 34443.0 53 33205.0 54 31814.5 55 29426.5 56 26462.0 57 23762.5 58 21553.5 59 19234.5 60 16864.0 61 14622.5 62 12538.0 63 9801.5 64 7139.0 65 5413.5 66 4033.0 67 3368.5 68 2603.0 69 1684.5 70 1381.5 71 1185.0 72 1031.5 73 909.5 74 734.5 75 589.5 76 396.0 77 198.5 78 115.5 79 89.5 80 59.0 81 26.5 82 12.0 83 8.0 84 8.5 85 8.0 86 3.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3429457443518574 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001028482583888714 16-17 0.0018441756676625216 18-19 3.546491668581772E-5 20-21 0.0 22-23 0.0 24-25 2.4825441680072403E-4 26-27 8.511580004596254E-4 28-29 0.0 30-31 0.002943588084922871 32-33 0.0 34-35 0.010249360922201321 36-37 0.027272520931393828 38-39 0.020605116594460095 40-41 0.013973177174212183 42-43 0.010249360922201321 44-45 0.0027662635014937824 46-47 8.156930837738076E-4 48-49 4.255790002298127E-4 50-51 3.546491668581772E-5 52-53 3.546491668581772E-5 54-55 0.0 56-57 0.0 58-59 0.004113930335554856 60-61 0.010072036338772234 62-63 0.004007535585497403 64-65 0.0017377809176050685 66-67 0.0036174215019534077 68-69 0.0031918425017235953 70-71 3.546491668581772E-5 72-73 0.0 74-75 0.0 76-77 0.21069707003044308 78-79 4.255790002298127E-4 80-81 0.0031918425017235953 82-83 3.546491668581772E-4 84-85 0.0 86-87 0.10281279347218557 88-89 0.3894757150436502 90-91 0.3770984591202998 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0011703422506319848 100-101 6.383685003447189E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1409844.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.90261670497624 #Duplication Level Percentage of deduplicated Percentage of total 1 69.47154698075605 24.94210323151833 2 14.457912665325606 10.381537935544133 3 5.802384323392732 6.249623410131964 4 2.9390716807464026 4.220814560891535 5 1.7786392746894426 3.1928902067796 6 1.1334728728270407 2.4416785259158504 7 0.7537562113387943 1.8943274241284256 8 0.5281534026664432 1.5169671341889825 9 0.40588162812365364 1.3114991269903717 >10 2.301332171959481 15.825958162172007 >50 0.25860056032055806 6.4616849837183254 >100 0.15621394499667693 9.704812822023614 >500 0.006122163160176471 1.412757555194178 >1k 0.005727184891777989 4.826844834499315 >5k 3.94978268398482E-4 1.0947541607125777 >10k+ 7.89956536796964E-4 4.521745925590881 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22007 1.5609528430095811 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16193 1.148566791786893 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12950 0.918541342162679 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12623 0.8953472866501542 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9376 0.6650381176924539 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6064 0.43011850956559733 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4806 0.34088877918407995 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4719 0.3347178836807477 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4143 0.29386229965868566 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3966 0.28130771915190617 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3685 0.26137643597447663 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3338 0.23676378379451912 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3244 0.2300963794575854 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2904 0.20598023611122931 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2882 0.20441977977705333 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2881 0.2043488499436817 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2784 0.19746865610663308 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2692 0.19094311143644263 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2516 0.1784594607630348 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2375 0.1684583542576342 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2285 0.16207466925418698 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1952 0.1384550347414324 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1896 0.1344829640726208 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1529 0.10845171522523059 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1484 0.105259872723507 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1470 0.10426685505630409 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1436 0.10185524072166849 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.4185966674327088E-4 0.0 10-11 0.0 0.0 0.0 1.4185966674327088E-4 0.0 12-13 0.0 0.0 0.0 1.4185966674327088E-4 0.0 14-15 0.0 0.0 0.0 1.4185966674327088E-4 0.0 16-17 0.0 0.0 0.0 2.8371933348654175E-4 0.0 18-19 0.0 0.0 0.0 2.8371933348654175E-4 0.0 20-21 0.0 0.0 0.0 7.092983337163544E-4 0.0 22-23 0.0 0.0 0.0 0.0018087107509767038 0.0 24-25 0.0 0.0 0.0 0.003049982834980324 0.0 26-27 0.0 0.0 0.0 0.004681369002527939 0.0 28-29 0.0 0.0 0.0 0.010320290755572958 0.0 30-31 0.0 0.0 0.0 0.023903353846241142 0.0 32-33 0.0 0.0 0.0 0.04805496210928301 0.0 34-35 0.0 0.0 0.0 0.08160477329406658 0.0 36-37 0.0 0.0 0.0 0.13515679748965134 0.0 38-39 0.0 0.0 0.0 0.2166906409503463 0.0 40-41 0.0 0.0 0.0 0.31361625825268613 0.0 42-43 0.0 0.0 0.0 0.4084139805538769 0.0 44-45 0.0 0.0 0.0 0.5194191697804864 0.0 46-47 0.0 0.0 0.0 0.6524480722689886 0.0 48-49 0.0 0.0 0.0 0.7931019318449417 0.0 50-51 0.0 0.0 0.0 0.9572335662669061 0.0 52-53 0.0 0.0 0.0 1.1196274197712655 0.0 54-55 0.0 0.0 0.0 1.2996473368684762 0.0 56-57 0.0 0.0 0.0 1.5118693983164095 0.0 58-59 0.0 0.0 0.0 1.7484558575274995 0.0 60-61 0.0 0.0 0.0 2.0202944439242922 0.0 62-63 0.0 0.0 0.0 2.290749898570338 0.0 64-65 0.0 0.0 0.0 2.5659931169689694 0.0 66-67 0.0 0.0 0.0 2.8808506473056594 0.0 68-69 0.0 0.0 0.0 3.240500367416537 0.0 70-71 0.0 0.0 0.0 3.636466162213692 0.0 72-73 0.0 0.0 0.0 4.0315453340937015 0.0 74-75 0.0 0.0 0.0 4.448719149068975 0.0 76-77 0.0 0.0 0.0 4.886746335055509 0.0 78-79 0.0 0.0 0.0 5.400562047999637 0.0 80-81 0.0 0.0 0.0 5.979845997145784 0.0 82-83 0.0 0.0 0.0 6.5423550406995385 0.0 84-85 0.0 3.546491668581772E-5 0.0 7.1183407525939035 0.0 86-87 0.0 7.092983337163544E-5 0.0 7.703405483159839 0.0 88-89 0.0 7.092983337163544E-5 0.0 8.336383316168314 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12365 0.0 25.141994 1 TACATGG 12645 0.0 24.243225 2 GAGTACT 12990 0.0 23.491425 12-13 ACATGGG 12405 0.0 23.488125 3 AGTACTT 13530 0.0 22.55385 12-13 GTACTTT 13825 0.0 21.986774 14-15 CATGGGA 8765 0.0 21.710468 4 GTAAGCG 210 0.0 21.46748 94-95 TGTACCG 155 3.9362563E-5 21.431032 5 ATGGGAT 3375 0.0 21.231462 5 CATGGGG 2895 0.0 20.981579 4 ATGGGAG 2620 0.0 20.829233 5 TACTTTT 14980 0.0 20.22817 14-15 ACTTTTT 15220 0.0 20.002031 16-17 AGAGTAC 21370 0.0 19.698204 10-11 AGTACAT 9360 0.0 19.569881 2 GTATCAA 28710 0.0 19.434574 1 GTATAGG 550 0.0 19.04323 1 CTAGACA 285 4.321373E-8 18.315742 4 ATGGGGA 1455 0.0 18.264246 5 >>END_MODULE