##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939078_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1392453 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.3615942512961 33.0 31.0 34.0 31.0 34.0 2 32.43119300974611 34.0 31.0 34.0 31.0 34.0 3 32.46911170430887 34.0 31.0 34.0 31.0 34.0 4 35.925282935941105 37.0 35.0 37.0 35.0 37.0 5 35.98687208832183 37.0 35.0 37.0 35.0 37.0 6 36.108015854036005 37.0 37.0 37.0 35.0 37.0 7 36.05844146983776 37.0 37.0 37.0 35.0 37.0 8 36.06644461249321 37.0 37.0 37.0 35.0 37.0 9 37.764357576162354 39.0 38.0 39.0 35.0 39.0 10-11 37.69380510509151 39.0 38.0 39.0 35.0 39.0 12-13 37.61180880072793 39.0 38.0 39.0 35.0 39.0 14-15 38.863123925906294 40.0 38.0 41.0 35.5 41.0 16-17 38.83002191097294 40.0 38.0 41.0 35.5 41.0 18-19 38.82432620706049 40.0 38.5 41.0 35.0 41.0 20-21 38.83089447184214 40.5 39.0 41.0 35.0 41.0 22-23 38.82887070515127 41.0 39.0 41.0 35.0 41.0 24-25 38.76226414823337 41.0 38.5 41.0 35.0 41.0 26-27 38.6193422686439 40.0 38.0 41.0 35.0 41.0 28-29 38.47253085023337 40.0 38.0 41.0 35.0 41.0 30-31 38.17947284396672 40.0 37.5 41.0 34.5 41.0 32-33 37.86325463049741 40.0 36.5 41.0 34.0 41.0 34-35 37.519035112854795 40.0 36.0 41.0 33.5 41.0 36-37 37.225523949461845 40.0 35.5 41.0 33.0 41.0 38-39 37.01191961236752 40.0 35.0 41.0 33.0 41.0 40-41 36.81009125622194 40.0 35.0 41.0 32.5 41.0 42-43 36.53148472515769 39.5 35.0 41.0 31.0 41.0 44-45 36.14284611401605 39.0 35.0 41.0 30.5 41.0 46-47 35.73704211201384 39.0 35.0 41.0 28.5 41.0 48-49 35.459472599793315 39.0 35.0 41.0 26.5 41.0 50-51 34.28261887474837 38.0 34.0 40.0 24.5 40.5 52-53 34.445377330509544 38.5 34.5 39.5 24.0 40.5 54-55 34.9490690170512 39.0 35.0 41.0 24.5 41.0 56-57 34.845917600091354 39.0 35.0 41.0 24.0 41.0 58-59 34.654003402628305 39.0 35.0 41.0 23.0 41.0 60-61 34.346297146115525 38.0 35.0 40.5 22.5 41.0 62-63 34.00558510771997 37.5 35.0 40.0 21.5 41.0 64-65 33.64404723175576 37.0 34.0 40.0 19.5 41.0 66-67 33.24020882571979 36.5 34.0 39.5 16.5 41.0 68-69 32.827073876102105 36.0 34.0 39.0 10.5 41.0 70-71 32.37200896547316 35.0 33.0 39.0 7.0 40.5 72-73 31.92428828836593 35.0 33.0 38.0 2.0 40.0 74-75 31.49389889640799 35.0 32.5 37.0 2.0 39.5 76-77 31.06913949698841 35.0 32.0 37.0 2.0 39.0 78-79 30.63425264622935 35.0 31.5 36.0 2.0 39.0 80-81 30.168780202994284 35.0 31.0 36.0 2.0 37.5 82-83 29.782874538673838 35.0 31.0 35.0 2.0 37.0 84-85 29.388594444480354 35.0 30.5 35.0 2.0 37.0 86-87 29.007670276842376 34.0 30.0 35.0 2.0 36.0 88-89 28.571847308311305 34.0 29.5 35.0 2.0 36.0 90-91 28.142838573366568 34.0 29.0 35.0 2.0 35.5 92-93 27.729426056032054 34.0 28.5 35.0 2.0 35.0 94-95 27.24751786954389 34.0 27.0 35.0 2.0 35.0 96-97 26.680532484758913 34.0 25.5 35.0 2.0 35.0 98-99 26.067763507996318 34.0 24.0 35.0 2.0 35.0 100-101 23.407155214574566 30.0 9.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26255428112285983 1101 2 0.35793855724276114 1101 3 0.24069638143909344 1101 4 0.33341718600132 1101 5 0.24753435166583415 1101 6 0.27126616865308506 1101 7 0.24834908911670794 1101 8 0.3007765613636977 1101 9 0.266180537635627 1101 10-11 0.32856055574679743 1101 12-13 0.333141089905439 1101 14-15 0.4698877451813601 1101 16-17 0.4747378614824598 1101 18-19 0.5062730215702729 1101 20-21 0.45684859014409795 1101 22-23 0.5357926855944228 1101 24-25 0.4477173955822451 1101 26-27 0.3661115805707098 1101 28-29 0.42082550988287437 1101 30-31 0.3315981019731069 1101 32-33 0.3723981963968086 1101 34-35 0.3626751081204631 1101 36-37 0.4220095139348885 1101 38-39 0.3128924354679512 1101 40-41 0.2659965357078349 1101 42-43 0.33736252174048786 1101 44-45 0.32080078412187873 1101 46-47 0.3946825203127986 1101 48-49 0.37501735792948665 1101 50-51 0.31342044328760466 1101 52-53 0.42434476325450277 1101 54-55 0.40794150086240677 1101 56-57 0.43124024653348414 1101 58-59 0.39053052265987276 1101 60-61 0.4149942592556499 1101 62-63 0.25139505422981756 1101 64-65 0.3120524932159938 1101 66-67 0.3199823947539926 1101 68-69 0.3568495967112071 1101 70-71 0.33417377642764023 1101 72-73 0.238345257955217 1101 74-75 0.26699982616304396 1101 76-77 0.24124622281481933 1101 78-79 0.12959182981895978 1101 80-81 -0.021618456354364213 1101 82-83 -0.01724859932969025 1101 84-85 0.062437564951054014 1101 86-87 -0.04801186014099912 1101 88-89 0.024654310429941262 1101 90-91 -0.08844498615895802 1101 92-93 -0.3812479221017071 1101 94-95 -0.4252160354364172 1101 96-97 -0.46580186091457065 1101 98-99 -0.5706043757315982 1101 100-101 -0.9443296974912414 1102 1 0.3771616378477063 1102 2 0.40408119839037937 1102 3 0.3540499121507352 1102 4 0.3144608685053427 1102 5 0.36367565955954717 1102 6 0.3684305929001468 1102 7 0.40091021313484987 1102 8 0.36718537105163307 1102 9 0.48784109119129937 1102 10-11 0.4972299399820628 1102 12-13 0.5467195008369856 1102 14-15 0.6524428270062685 1102 16-17 0.5381918826613301 1102 18-19 0.4893470795696615 1102 20-21 0.48911085253413944 1102 22-23 0.48495777079502034 1102 24-25 0.5580360007149849 1102 26-27 0.605055147053271 1102 28-29 0.5523573902531211 1102 30-31 0.6641953089480026 1102 32-33 0.6753964574933065 1102 34-35 0.55995697217962 1102 36-37 0.4959345425147461 1102 38-39 0.3733076305910785 1102 40-41 0.4214933004395789 1102 42-43 0.3166399974086005 1102 44-45 0.33068352869596396 1102 46-47 0.46831004888555583 1102 48-49 0.45419100999560413 1102 50-51 0.3968420692332941 1102 52-53 0.38699237857387914 1102 54-55 0.39459335990058264 1102 56-57 0.40605947716088764 1102 58-59 0.41049529071624136 1102 60-61 0.4185369560027894 1102 62-63 0.3657839842510029 1102 64-65 0.4566410425506504 1102 66-67 0.398300778623323 1102 68-69 0.38050102456940493 1102 70-71 0.2167641043797346 1102 72-73 0.1244467242508378 1102 74-75 0.15745316085799743 1102 76-77 0.0029955228565192726 1102 78-79 -0.07785325855875413 1102 80-81 0.16812130383013724 1102 82-83 -0.1807491425597405 1102 84-85 -0.1715094906853487 1102 86-87 -0.1738079120074154 1102 88-89 -0.1693071840625855 1102 90-91 -0.13760305050122312 1102 92-93 -0.21713784071648234 1102 94-95 -0.5692124214673235 1102 96-97 -0.6052261788984659 1102 98-99 -0.7823258050932544 1102 100-101 -0.8173943517955209 1103 1 0.1410533581895379 1103 2 0.20271668295452372 1103 3 0.2435057038341597 1103 4 0.23982713607488648 1103 5 0.23976592199391433 1103 6 0.20867787516447578 1103 7 0.21259992017589013 1103 8 0.17677582357597288 1103 9 0.33715208390176343 1103 10-11 0.27123913041916126 1103 12-13 0.16914226645329222 1103 14-15 0.2193186844773578 1103 16-17 0.35237548753865866 1103 18-19 0.3309401437649697 1103 20-21 0.261916828522196 1103 22-23 0.44353812926615177 1103 24-25 0.3279837397462444 1103 26-27 0.34802250667596013 1103 28-29 0.4337591523045532 1103 30-31 0.46053593916514757 1103 32-33 0.4020525819719367 1103 34-35 0.4129086011680698 1103 36-37 0.4266612096589384 1103 38-39 0.4555167240547391 1103 40-41 0.37788211433323227 1103 42-43 0.5219034235462487 1103 44-45 0.651219995667141 1103 46-47 0.7327747940823457 1103 48-49 0.6110241936445604 1103 50-51 0.5621135948568678 1103 52-53 0.5464461867097299 1103 54-55 0.5859988135544469 1103 56-57 0.6935443939345376 1103 58-59 0.6921452717072469 1103 60-61 0.6082928970032668 1103 62-63 0.41392633684862545 1103 64-65 0.4882069982624344 1103 66-67 0.4836807932795324 1103 68-69 0.37855948963417774 1103 70-71 0.46508428752729714 1103 72-73 0.2654170371554727 1103 74-75 0.27496458003119173 1103 76-77 0.38066504730099027 1103 78-79 0.5052022862832004 1103 80-81 0.525891300469123 1103 82-83 0.42476938983545054 1103 84-85 0.4141177786859238 1103 86-87 0.3758992392325773 1103 88-89 0.4294789950187017 1103 90-91 0.39085259241613457 1103 92-93 0.3166224144999745 1103 94-95 0.33034706567543637 1103 96-97 0.17365923266010697 1103 98-99 0.20705532806339733 1103 100-101 -0.05250328161650586 1104 1 0.2743887881917999 1104 2 0.3284887143724333 1104 3 0.23986887544294433 1104 4 0.2600702515286315 1104 5 0.30559619619806 1104 6 0.21214875444051273 1104 7 0.3247614475501379 1104 8 0.2649423133640809 1104 9 0.3687520073510413 1104 10-11 0.3835080563135165 1104 12-13 0.3525116892581792 1104 14-15 0.39567575344125316 1104 16-17 0.45511404942897826 1104 18-19 0.5187851740573777 1104 20-21 0.47345176415668533 1104 22-23 0.5692649930821503 1104 24-25 0.4433180402238861 1104 26-27 0.4573515235478993 1104 28-29 0.5402516437762444 1104 30-31 0.5962746201641096 1104 32-33 0.4182035126921946 1104 34-35 0.5453985605774534 1104 36-37 0.38062973553562074 1104 38-39 0.26732172849120417 1104 40-41 0.3105158070891534 1104 42-43 0.18321834277318771 1104 44-45 0.35975178763654725 1104 46-47 0.7070322721643478 1104 48-49 0.5831440731440907 1104 50-51 0.5978930270700005 1104 52-53 0.6014834115051997 1104 54-55 0.5868181873106693 1104 56-57 0.6618016070332757 1104 58-59 0.6635103503925563 1104 60-61 0.5830703043695422 1104 62-63 0.5817136061425572 1104 64-65 0.6888026752879881 1104 66-67 0.5591364507391461 1104 68-69 0.3841793430368696 1104 70-71 0.41622364864996797 1104 72-73 0.4809467962482046 1104 74-75 0.36988479731746793 1104 76-77 0.1789445297003489 1104 78-79 0.038110731888693294 1104 80-81 0.12509608301555986 1104 82-83 0.1517710506649017 1104 84-85 0.25426461688687496 1104 86-87 0.31768756845956503 1104 88-89 0.03560412332814877 1104 90-91 0.12120100526006539 1104 92-93 0.05822425171727019 1104 94-95 -0.040541846113246294 1104 96-97 -0.0015010621404663027 1104 98-99 0.11977746902111264 1104 100-101 -0.1774800286658902 1105 1 0.09391308156686051 1105 2 0.13622155907820144 1105 3 0.19284804956483725 1105 4 0.15185561412041437 1105 5 0.19877443468348588 1105 6 0.19894549251193183 1105 7 0.10190979373039966 1105 8 0.18490843506995702 1105 9 0.22967076997051095 1105 10-11 0.21807057704666022 1105 12-13 0.17774037427362543 1105 14-15 0.27851419028579727 1105 16-17 0.2629644364042534 1105 18-19 0.3206846571325954 1105 20-21 0.3019435531761516 1105 22-23 0.3079032267091648 1105 24-25 0.4842772765648178 1105 26-27 0.5044921772396407 1105 28-29 0.4529934869957515 1105 30-31 0.5392114398882271 1105 32-33 0.417701999008969 1105 34-35 0.4233991535129036 1105 36-37 0.4768341650507466 1105 38-39 0.5276256144386338 1105 40-41 0.5447377164457805 1105 42-43 0.47768458332735975 1105 44-45 0.5662275177699385 1105 46-47 0.5070243054105248 1105 48-49 0.43320062403596893 1105 50-51 0.31863120558275426 1105 52-53 0.45575319974626893 1105 54-55 0.5842851514028098 1105 56-57 0.4610041480359328 1105 58-59 0.4394032798332361 1105 60-61 0.3859564894959959 1105 62-63 0.43278787718458034 1105 64-65 0.524049553234704 1105 66-67 0.5892736356279187 1105 68-69 0.5307271265814748 1105 70-71 0.35708715334251195 1105 72-73 0.3847906896661044 1105 74-75 0.2519791262947706 1105 76-77 0.14217730779882487 1105 78-79 0.16896760197313654 1105 80-81 0.26757527150373406 1105 82-83 0.3458574990860477 1105 84-85 0.2896673689432099 1105 86-87 0.40142141619269367 1105 88-89 0.41118889611894005 1105 90-91 0.5208538637574556 1105 92-93 0.4343572285927877 1105 94-95 0.38068622245830497 1105 96-97 0.5680147420706803 1105 98-99 0.7903884406777522 1105 100-101 0.4524668683487967 1106 1 0.07117501577413066 1106 2 0.10769323852415624 1106 3 0.13879907603033104 1106 4 0.2193772564652079 1106 5 0.1835753251113843 1106 6 0.12715968989011373 1106 7 0.21034165126194182 1106 8 0.06477734429577708 1106 9 0.11436283032173122 1106 10-11 0.10010619416266309 1106 12-13 0.17977970150587907 1106 14-15 0.06806155610325959 1106 16-17 0.07633569537036067 1106 18-19 0.04824340063989041 1106 20-21 0.06681988263446925 1106 22-23 0.10087750323649658 1106 24-25 0.05126342546414975 1106 26-27 0.06373407681915921 1106 28-29 0.12339417955078602 1106 30-31 0.1099979845332868 1106 32-33 0.15713558792808868 1106 34-35 0.15330145378497662 1106 36-37 0.16280745221374815 1106 38-39 0.05024742992218734 1106 40-41 0.3383662101385738 1106 42-43 0.331372438880166 1106 44-45 0.4817370404016472 1106 46-47 0.5243468381832201 1106 48-49 0.6681709431059701 1106 50-51 0.5873771862901478 1106 52-53 0.47737699395849376 1106 54-55 0.5682722896664743 1106 56-57 0.50587553067259 1106 58-59 0.6762645214854786 1106 60-61 0.6173329426248699 1106 62-63 0.5495130812409741 1106 64-65 0.4758885341894441 1106 66-67 0.42072552778720507 1106 68-69 0.6184441683821191 1106 70-71 0.5092390207676942 1106 72-73 0.4822393663913047 1106 74-75 0.6199113548379316 1106 76-77 0.658681141585614 1106 78-79 0.7012604538158271 1106 80-81 0.6323353506528804 1106 82-83 0.7543511918884249 1106 84-85 0.8370356598163262 1106 86-87 0.5042188438454289 1106 88-89 0.4415623811548066 1106 90-91 0.3664437722412508 1106 92-93 0.16356450042064097 1106 94-95 0.20235948488719302 1106 96-97 0.14216512544935966 1106 98-99 0.06285814139503998 1106 100-101 -0.086407729721941 1107 1 0.023730564849770985 1107 2 0.04121572059099066 1107 3 0.05619453879349834 1107 4 0.048752074217581765 1107 5 0.08170910944684096 1107 6 -0.009195357640066959 1107 7 0.09550756949836625 1107 8 0.06412183604360422 1107 9 -0.004753633199584328 1107 10-11 0.08682223778545506 1107 12-13 0.10237665724157807 1107 14-15 0.06786851942045757 1107 16-17 0.14176463337701506 1107 18-19 0.0776024721976043 1107 20-21 0.16489359815322047 1107 22-23 0.13549842817963054 1107 24-25 -0.014319475334822585 1107 26-27 0.09633103255485764 1107 28-29 0.08481775218739784 1107 30-31 0.018953778549736455 1107 32-33 -0.0453412301742091 1107 34-35 2.291962472185105E-4 1107 36-37 0.033903853916662285 1107 38-39 -0.06194945583042255 1107 40-41 0.04751008370246268 1107 42-43 0.009182551436296649 1107 44-45 -0.1393844385742682 1107 46-47 -0.11799672045677312 1107 48-49 -0.19355793074951322 1107 50-51 -0.26417323069241405 1107 52-53 -0.07002634190200752 1107 54-55 -0.3857307429339869 1107 56-57 -0.4760767058702484 1107 58-59 -0.36902882428930184 1107 60-61 -0.48101924726258005 1107 62-63 -0.41293253606817615 1107 64-65 -0.4154635973787606 1107 66-67 -0.5182136452459787 1107 68-69 -0.5260744062956704 1107 70-71 -0.4761359719424014 1107 72-73 -0.3905701629724305 1107 74-75 -0.3086811103163214 1107 76-77 -0.43197757422472804 1107 78-79 -0.30106444266752064 1107 80-81 -0.33912612948941856 1107 82-83 -0.3588412032827435 1107 84-85 -0.37026969972178847 1107 86-87 -0.554067736897558 1107 88-89 -0.49063858694456997 1107 90-91 -0.3269066680690038 1107 92-93 -0.31804977612919316 1107 94-95 -0.36834384514138563 1107 96-97 -0.04599665212106174 1107 98-99 -0.08401186502007718 1107 100-101 0.2828947721734316 1108 1 -0.06395432140818968 1108 2 0.020735067923602912 1108 3 0.017294576115240545 1108 4 0.011514574629011065 1108 5 0.04197233648852716 1108 6 -0.0023971109249600886 1108 7 -0.13695330543698958 1108 8 -0.03816260023356932 1108 9 -0.02113782060938263 1108 10-11 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-0.6773434148186226 2316 60-61 -0.5069997058062796 2316 62-63 -0.5823290916112143 2316 64-65 -0.6974749776465217 2316 66-67 -0.7095501453432291 2316 68-69 -0.6790293986740892 2316 70-71 -0.7070477392168968 2316 72-73 -1.2136039194269372 2316 74-75 -0.873463286658577 2316 76-77 -0.8551485318016496 2316 78-79 -0.6684366909981776 2316 80-81 -0.5845838736953937 2316 82-83 -0.6034815207523039 2316 84-85 -0.5669879265027546 2316 86-87 -0.629025452203539 2316 88-89 -0.6947267331810529 2316 90-91 -0.8098303598592587 2316 92-93 -0.6013907669052472 2316 94-95 -0.5358173911027073 2316 96-97 -0.6108775525181898 2316 98-99 -1.152562431354415 2316 100-101 -0.9929575349528861 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4002.0 3 789.0 4 703.0 5 744.0 6 815.0 7 1091.0 8 1217.0 9 1683.0 10 2173.0 11 3112.0 12 5212.0 13 9309.0 14 8137.0 15 9387.0 16 13200.0 17 21409.0 18 16710.0 19 9157.0 20 8586.0 21 8618.0 22 8802.0 23 9432.0 24 10446.0 25 11630.0 26 13022.0 27 15004.0 28 17569.0 29 20956.0 30 25160.0 31 30818.0 32 38684.0 33 51474.0 34 73679.0 35 132388.0 36 177817.0 37 275273.0 38 295853.0 39 58387.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.24892749343808 20.226633918961685 15.024103012534143 19.50033557506609 2 14.9707913477681 22.87707920667503 40.19894237717088 21.953187068385994 3 19.595494792696474 29.239557531986183 30.19128796730022 20.973659708017127 4 11.688129587191423 17.835927843168836 39.00041202479627 31.475530544843473 5 11.790635114330358 40.26928163041657 33.114492871385806 14.825590383867263 6 27.487966033239474 38.195788938093166 18.26697942763019 16.04926560103717 7 25.717333072678777 36.3265963234771 21.667796249593028 16.2882743542511 8 25.708045344224995 33.0675340897412 20.700071366917232 20.524349199116575 9 27.442351850454394 17.858007855312756 22.07184772336075 32.6277925708721 10-11 24.280048482479003 28.07039230489773 26.47767890670788 21.171880305915387 12-13 26.78323666082954 26.549655863614692 27.524190885102325 19.142916590453446 14-15 23.09202623294368 26.940152279284334 28.820088878092847 21.14773260967914 16-17 19.461508083523455 30.536928789316693 28.96945362057981 21.032109506580046 18-19 19.210672144426564 28.554584377603188 33.18366763145582 19.051075846514433 20-21 21.144550278240466 26.597129011217664 32.852211818526484 19.40610889201539 22-23 20.16347628073671 27.032132437135704 33.13153073113999 19.672860550987593 24-25 20.388045205562666 27.306512075460983 33.2817885535363 19.02365416544005 26-27 19.768799840402878 27.027701244246504 34.162088134277255 19.041410781073363 28-29 19.0068632860026 28.166645400484153 33.82289882628644 19.00359248722681 30-31 19.374527623448635 27.590995492139548 33.26925364594733 19.76522323846449 32-33 19.21144521417369 28.082039656310066 33.34459415750107 19.361920972015177 34-35 20.10565179077732 27.650499581730447 33.048122254317974 19.19572637317426 36-37 19.97988108399593 27.236839778136073 33.52336938062686 19.259909757241132 38-39 19.521987113211395 28.176380476028086 33.24906939775645 19.05256301300407 40-41 19.22232997239383 28.297299559426865 33.278099344696166 19.20227112348314 42-43 19.08190698899703 28.562685172153717 32.02351438253094 20.33189345631832 44-45 19.452515769008254 27.98587980365117 32.08349408813321 20.478110339207365 46-47 20.20696161325109 28.557173028368172 31.612406581080478 19.623458777300268 48-49 19.9688163757338 28.65645138296125 31.362208262577667 20.01252397872728 50-51 19.289917934747695 29.045353521573077 31.666042280676297 19.99868626300293 52-53 19.741713065607204 29.73259268268827 30.394262568136693 20.131431683567826 54-55 19.23814535081793 28.94976931649546 30.795850872786275 21.016234459900335 56-57 20.01874700989522 29.042784024776648 30.20660676956115 20.731862195766986 58-59 20.518538052855988 28.52139669337112 30.527820337113226 20.432244916659663 60-61 20.03798971796889 29.170269367869267 31.30632563418959 19.48541527997225 62-63 19.68611876795861 29.213442196685097 31.16339137221733 19.937047663138962 64-65 19.402124755195484 29.56632154012628 30.94343490780624 20.08811879687199 66-67 19.839374738972218 29.120322391193156 30.12010616904529 20.920196700789337 68-69 19.3444643532139 29.117676472106684 30.75729180242879 20.780567372250623 70-71 20.292344459606788 29.07787669403109 30.237146302192762 20.392632544169356 72-73 19.919245472898755 28.74892929502944 30.783133378164873 20.54869185390693 74-75 19.07845649205299 30.245386127863288 30.165502707842183 20.51065467224154 76-77 19.471717560309816 29.081662481567637 30.3043152833946 21.142304674727942 78-79 19.39602763696715 29.025057276351628 30.61945050782025 20.959464578860967 80-81 19.89118907936132 29.46673772548516 29.934585714940358 20.707487480213164 82-83 19.80994365720427 28.84189626184514 30.391548688901377 20.95661139204922 84-85 19.36394033237371 28.943915635651905 30.266150118828616 21.42599391314577 86-87 19.50134399823326 29.165681837782714 30.126562088965503 21.20641207501852 88-89 19.433192828478216 28.764038455986373 29.90974247192309 21.893026243612322 90-91 19.730402861649633 28.205332746180193 30.111029695751203 21.953234696418967 92-93 20.255701446289194 28.607258086058284 30.043945301924513 21.093095165728005 94-95 19.818407517527387 28.063705363056823 30.34469291024802 21.77319420916777 96-97 19.2592434839358 28.077804376577397 30.802913964273543 21.860038175213255 98-99 19.737486139025574 28.410160671795225 29.80672603784046 22.04562715133874 100-101 19.854024608311395 27.233234031573268 30.66721039041651 22.24553096969883 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10349.0 1 6336.0 2 3507.5 3 4456.0 4 3452.0 5 3103.5 6 5335.0 7 10047.5 8 14505.0 9 14487.5 10 10140.5 11 6756.0 12 6142.0 13 6068.0 14 5882.5 15 5671.0 16 5431.5 17 5299.0 18 5215.5 19 5198.0 20 5355.0 21 5672.5 22 6265.5 23 7043.0 24 7532.5 25 8291.5 26 9721.5 27 11419.5 28 12574.0 29 14186.0 30 16991.5 31 19878.0 32 23674.0 33 27970.0 34 31702.0 35 34239.5 36 38591.5 37 43255.5 38 43983.5 39 43498.0 40 42004.0 41 40555.5 42 39319.0 43 40393.5 44 42048.0 45 42273.5 46 41578.0 47 46444.5 48 65831.5 49 64923.0 50 45421.0 51 36944.0 52 33222.0 53 31975.5 54 31076.0 55 29048.5 56 26013.0 57 23420.5 58 21245.0 59 18822.0 60 16575.5 61 14663.5 62 12579.0 63 9692.5 64 7079.5 65 5552.0 66 4234.5 67 3457.0 68 2639.0 69 1770.5 70 1470.0 71 1255.5 72 1114.5 73 968.5 74 743.5 75 609.0 76 464.0 77 295.5 78 188.5 79 141.0 80 100.0 81 64.5 82 42.5 83 37.5 84 29.5 85 21.5 86 15.5 87 8.0 88 5.0 89 4.5 90 6.0 91 5.5 92 3.0 93 1.5 94 0.5 95 0.5 96 1.5 97 1.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3793305770464066 2 0.11476150361987084 3 0.11619781780785421 4 0.12639564854253607 5 0.12855011982451114 6 0.07002031666418902 7 0.07784822898869836 8 0.07555012628792498 9 0.07454470635633663 10-11 0.07343156286064952 12-13 0.07695053262120875 14-15 0.07156435441627114 16-17 0.07264159005725866 18-19 0.0811517516210601 20-21 0.080469502381768 22-23 0.08919511107376694 24-25 0.10266055658611098 26-27 0.10416868648349352 28-29 0.09734619409057255 30-31 0.08736381048408816 32-33 0.08610703556960271 34-35 0.3297777375609805 36-37 0.5163190427253199 38-39 0.4973596954439396 40-41 0.46902839808596775 42-43 0.49682107762344585 44-45 0.5071266319222265 46-47 0.5046489899479552 48-49 0.5106456016827857 50-51 0.5093170110589011 52-53 0.509496550332399 54-55 0.5030690443411734 56-57 0.4762817847352837 58-59 0.4653298890519106 60-61 0.41380211755800733 62-63 0.48845454747844275 64-65 0.5144877421356412 66-67 0.5245060335968251 68-69 0.5403773053740414 70-71 0.5345243250580092 72-73 0.5219206680584552 74-75 0.5249369278532202 76-77 0.5014172830249926 78-79 0.47599452189768704 80-81 0.48450468346148845 82-83 0.496713354059347 84-85 0.4915067151279074 86-87 0.4918298858202036 88-89 0.5007709416404 90-91 0.5198021046311796 92-93 0.5258346242207098 94-95 0.5216334052208584 96-97 0.5214538659473605 98-99 0.5220283916225539 100-101 0.4987960096319229 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1392453.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.70413778542601 #Duplication Level Percentage of deduplicated Percentage of total 1 70.11024816768528 25.73336208917132 2 14.242294762128898 10.455022986596605 3 5.661985755196956 6.234549158936053 4 2.9641135721746195 4.3518093185899405 5 1.706159465470507 3.1311556052269145 6 1.0579056416918327 2.3297708661981926 7 0.7248475114190968 1.8623432052784776 8 0.496473264276568 1.45780984790299 9 0.39642544320789114 1.309540868023591 >10 2.2279523501468637 15.694684886542412 >50 0.25261111389318053 6.466154817624398 >100 0.1466023299885309 9.29318059313715 >500 0.005895534628484041 1.425147933914832 >1k 0.005109463344686169 4.344111725273543 >5k 5.895534628484042E-4 1.5247597011350154 >10k+ 7.860712837978721E-4 4.386596396448553 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22138 1.5898561746787863 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15270 1.0966258825252988 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11901 0.8546787575595011 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11506 0.8263115523468296 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9609 0.6900771516166075 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6304 0.4527262320523565 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5226 0.3753088973200532 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4446 0.31929264398870194 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4442 0.31900538115110527 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4171 0.2995433239039307 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4154 0.2983224568441448 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3281 0.23562734253867096 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3090 0.22191054204342983 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2863 0.20560837600981866 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2798 0.20094035489887271 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2629 0.18880350001041327 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2588 0.1858590559250474 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2588 0.1858590559250474 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2331 0.16740241860946115 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2208 0.15856908635336348 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2081 0.14944849125966908 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1988 0.14276963028554646 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1760 0.12639564854253607 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1489 0.10693359129536148 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1486 0.10671814416716399 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1473 0.1057845399449748 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1421 0.10205012305621805 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.0772356409875235E-4 0.0 20-21 0.0 0.0 0.0 2.1544712819750468E-4 0.0 22-23 0.0 0.0 0.0 6.822492392920982E-4 0.0 24-25 0.0 0.0 0.0 0.0020826555725758787 0.0 26-27 0.0 0.0 0.0 0.0041653111451517575 0.0 28-29 0.0 0.0 0.0 0.009443765785990623 0.0 30-31 0.0 0.0 0.0 0.023088750571832586 0.0 32-33 0.0 0.0 0.0 0.05156367934860279 0.0 34-35 0.0 0.0 0.0 0.08933874249256528 0.0 36-37 0.0 0.0 0.0 0.1460372450632086 0.0 38-39 0.0 0.0 0.0 0.23167747852171672 0.0 40-41 0.0 0.0 0.0 0.3277668976978038 0.0 42-43 0.0 0.0 0.0 0.42784208874554475 0.0 44-45 0.0 0.0 0.0 0.5401618582458438 0.0 46-47 0.0 0.0 0.0 0.6772939553435555 0.0 48-49 0.0 0.0 0.0 0.820925374141892 0.0 50-51 0.0 0.0 0.0 0.9829416145464156 0.0 52-53 0.0 0.0 0.0 1.1438806193099516 0.0 54-55 0.0 0.0 0.0 1.3177105439106382 0.0 56-57 0.0 0.0 0.0 1.5366766418687021 0.0 58-59 0.0 0.0 0.0 1.7766847426807224 0.0 60-61 0.0 0.0 0.0 2.048363571337776 0.0 62-63 0.0 0.0 0.0 2.3118913169780235 0.0 64-65 0.0 0.0 0.0 2.582816080686386 0.0 66-67 0.0 0.0 0.0 2.90192918540159 0.0 68-69 0.0 0.0 0.0 3.2644189785938913 0.0 70-71 0.0 0.0 0.0 3.6611289573148964 0.0 72-73 0.0 0.0 0.0 4.059454789497384 0.0 74-75 0.0 0.0 0.0 4.477350402491143 0.0 76-77 0.0 0.0 0.0 4.921530565124998 0.0 78-79 0.0 0.0 0.0 5.450166002012276 0.0 80-81 0.0 0.0 0.0 6.028246554820881 0.0 82-83 0.0 0.0 0.0 6.596416539732401 0.0 84-85 0.0 0.0 0.0 7.172881239079524 0.0 86-87 0.0 0.0 0.0 7.7756663959214425 0.0 88-89 0.0 0.0 0.0 8.405454259497448 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12845 0.0 27.1733 1 CATGGGG 3205 0.0 26.604486 4 TACATGG 12945 0.0 26.566195 2 ACATGGG 12730 0.0 25.08083 3 TTCGCGA 50 0.0016433888 23.776852 48-49 TCGCGAT 50 0.0016433888 23.776852 50-51 GAGTACT 12015 0.0 23.443932 12-13 GTACTTT 12820 0.0 21.935295 14-15 CGGTATA 110 0.0029241263 21.531393 2 AGTACTT 12445 0.0 21.41661 12-13 CATGGGA 8990 0.0 21.287865 4 ATGGGAC 1400 0.0 20.300297 5 CCCTATA 330 2.910383E-11 20.095966 2 ACTTTTT 14100 0.0 20.063684 16-17 ATATAGG 640 0.0 19.983826 2 CCTAGAC 430 0.0 19.829624 3 GTATCAA 27300 0.0 19.813 1 CATGGGT 1185 0.0 19.587912 4 GTATAGT 560 0.0 19.504236 1 CTTACTA 560 0.0 19.443266 9 >>END_MODULE