##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939078_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1392453 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.39016684943765 34.0 31.0 34.0 31.0 34.0 2 32.58328216464039 34.0 31.0 34.0 31.0 34.0 3 32.667113360379126 34.0 31.0 34.0 31.0 34.0 4 36.16501239180066 37.0 35.0 37.0 35.0 37.0 5 36.14428853253934 37.0 35.0 37.0 35.0 37.0 6 36.21518715532948 37.0 37.0 37.0 35.0 37.0 7 36.16593522366644 37.0 37.0 37.0 35.0 37.0 8 36.15678662044608 37.0 37.0 37.0 35.0 37.0 9 37.85299826995956 39.0 38.0 39.0 35.0 39.0 10-11 37.770501051022904 39.0 38.0 39.0 35.0 39.0 12-13 37.6801626338555 39.0 37.0 39.0 35.0 39.0 14-15 38.95005863752672 40.0 38.0 41.0 35.5 41.0 16-17 39.08291590452245 40.0 38.5 41.0 36.0 41.0 18-19 39.18609532960897 41.0 39.0 41.0 36.0 41.0 20-21 39.168137452395165 41.0 39.0 41.0 35.0 41.0 22-23 39.06722919911839 40.5 39.0 41.0 35.0 41.0 24-25 38.9553618685873 40.5 38.0 41.0 35.0 41.0 26-27 38.75378702189589 40.0 38.0 41.0 35.0 41.0 28-29 38.56791934808572 40.0 38.0 41.0 34.5 41.0 30-31 38.2593082136345 40.0 37.5 41.0 34.0 41.0 32-33 37.96943379776553 40.0 36.5 41.0 33.5 41.0 34-35 37.765115590975064 40.0 36.0 41.0 33.0 41.0 36-37 37.54037263735293 40.0 35.5 41.0 33.0 41.0 38-39 37.35960100628172 40.0 35.0 41.0 33.0 41.0 40-41 37.122362119224135 40.0 35.0 41.0 33.0 41.0 42-43 36.82641245341854 40.0 35.0 41.0 31.5 41.0 44-45 36.398821719655885 39.5 35.0 41.0 30.5 41.0 46-47 35.85812267990374 39.0 35.0 41.0 29.0 41.0 48-49 35.32901433657007 39.0 35.0 41.0 26.5 41.0 50-51 35.012857884610824 39.0 35.0 40.0 25.5 41.0 52-53 34.758873010435536 38.0 35.0 40.0 24.0 41.0 54-55 34.519636928499565 38.0 35.0 40.0 24.0 41.0 56-57 34.27738350953318 38.0 34.0 40.0 23.0 41.0 58-59 33.950971774271736 38.0 34.0 40.0 22.5 41.0 60-61 33.536843254314505 37.0 33.5 40.0 21.0 41.0 62-63 33.14744770559581 36.5 33.0 40.0 19.5 41.0 64-65 32.69691723885833 36.0 33.0 39.0 16.5 40.5 66-67 32.25755124230405 35.5 32.0 39.0 11.5 40.0 68-69 31.754475375470484 35.0 31.5 38.0 7.5 40.0 70-71 31.22761199121263 35.0 31.0 37.5 4.0 40.0 72-73 30.644024609807296 35.0 30.5 37.0 2.0 39.0 74-75 30.105595305550708 34.5 30.0 36.0 2.0 39.0 76-77 28.146769406220535 32.5 27.0 34.5 2.0 36.5 78-79 29.109254675023145 34.0 29.0 35.0 2.0 37.0 80-81 29.108265054547623 34.0 29.0 35.0 2.0 37.0 82-83 28.698044027338803 34.0 29.0 35.0 2.0 36.5 84-85 28.204670821923614 34.0 28.5 35.0 2.0 36.0 86-87 27.51197311507103 33.0 26.5 35.0 2.0 35.5 88-89 26.79519739624964 33.0 25.5 35.0 2.0 35.0 90-91 26.199835470209766 32.5 24.5 35.0 2.0 35.0 92-93 25.71403882213619 32.0 23.5 34.0 2.0 35.0 94-95 25.147014297789585 32.0 19.5 34.0 2.0 35.0 96-97 24.298331792886366 31.5 9.5 34.0 2.0 35.0 98-99 23.088042828016455 31.0 2.0 34.0 2.0 35.0 100-101 17.377110753468877 23.5 2.0 29.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1316116818832498 1101 2 0.19018083463887336 1101 3 0.2784847540983648 1101 4 0.14429337665992392 1101 5 0.13567723926402664 1101 6 0.15493307544754487 1101 7 0.22790489789442603 1101 8 0.2305177433531327 1101 9 0.26531253352465 1101 10-11 0.2990601692774604 1101 12-13 0.2494631778692522 1101 14-15 0.24672040022783648 1101 16-17 0.2972979760478225 1101 18-19 0.20044752589330272 1101 20-21 0.31485817215730094 1101 22-23 0.3724003982664925 1101 24-25 0.3158173376737139 1101 26-27 0.23905559649204378 1101 28-29 0.3272144187160535 1101 30-31 0.3937899241212506 1101 32-33 0.2920895069859881 1101 34-35 0.386273243106352 1101 36-37 0.36003272766074446 1101 38-39 0.34234682843704434 1101 40-41 0.31856952397771465 1101 42-43 0.2992819987397368 1101 44-45 0.3730291234506353 1101 46-47 0.5805488811777622 1101 48-49 0.5123516758619857 1101 50-51 0.28802443795049015 1101 52-53 0.31294866588558534 1101 54-55 0.4786632592431346 1101 56-57 0.6911333432024733 1101 58-59 0.5921809894897123 1101 60-61 0.8084198622218253 1101 62-63 0.5867221268144789 1101 64-65 0.5956190628843814 1101 66-67 0.6777118465437013 1101 68-69 0.8118996015157087 1101 70-71 0.5686732487669168 1101 72-73 0.5563509645232152 1101 74-75 0.45489035133858735 1101 76-77 0.7688668965987091 1101 78-79 0.8154740069136217 1101 80-81 0.7409616842902658 1101 82-83 0.715316006370113 1101 84-85 0.71711227401358 1101 86-87 0.8086040414711029 1101 88-89 0.9130143687730232 1101 90-91 0.9335449312894362 1101 92-93 0.8796579090893033 1101 94-95 0.5401393138995907 1101 96-97 0.7098106350575115 1101 98-99 0.7669905360168023 1101 100-101 0.017886705533875613 1102 1 0.20518034871444257 1102 2 0.20576075753708523 1102 3 0.16029817797949164 1102 4 0.13219906171131157 1102 5 0.19340287830397074 1102 6 0.2614997764384128 1102 7 0.2262383305126292 1102 8 0.24402190338899032 1102 9 0.3544483580570912 1102 10-11 0.31071195739788493 1102 12-13 0.3329383467244398 1102 14-15 0.3819034815194513 1102 16-17 0.3424059270635098 1102 18-19 0.36135212448458987 1102 20-21 0.3635300952484073 1102 22-23 0.3117735136269886 1102 24-25 0.39428925849545493 1102 26-27 0.4430409619028879 1102 28-29 0.47174233336510696 1102 30-31 0.34808342720333485 1102 32-33 0.4841209746148607 1102 34-35 0.608712119088203 1102 36-37 0.6645330269336966 1102 38-39 0.5521336212805892 1102 40-41 0.784481483832586 1102 42-43 0.8111692338264476 1102 44-45 0.8293008774737984 1102 46-47 0.968016223548851 1102 48-49 1.0860664332806849 1102 50-51 1.0629566405710378 1102 52-53 1.0940081630502902 1102 54-55 0.9914986647934398 1102 56-57 1.1370347930451814 1102 58-59 0.8042343139725077 1102 60-61 0.505086514704935 1102 62-63 0.5761511253705933 1102 64-65 0.7146208386651125 1102 66-67 0.8064963728556052 1102 68-69 0.8829964297897668 1102 70-71 0.8732636135962473 1102 72-73 0.9165912055301355 1102 74-75 0.780899298323309 1102 76-77 0.7251170207047899 1102 78-79 0.8601117731743528 1102 80-81 0.8935391193934201 1102 82-83 0.7890913984744152 1102 84-85 0.7168367585123789 1102 86-87 0.9786233452856301 1102 88-89 0.9253682427266305 1102 90-91 1.2921267604570623 1102 92-93 1.0013261926809207 1102 94-95 1.0690829796670194 1102 96-97 0.9424574135473058 1102 98-99 0.707595492884618 1102 100-101 0.4644430808987501 1103 1 0.02489515865201497 1103 2 0.07031338491113814 1103 3 0.12637262713180064 1103 4 0.11360881305158443 1103 5 0.1280890645480639 1103 6 0.11747218308770613 1103 7 0.05624279349466832 1103 8 0.19501450365456208 1103 9 0.18382332017684178 1103 10-11 0.14237075415589118 1103 12-13 0.155329568424456 1103 14-15 0.02483668004500572 1103 16-17 0.10535683977088439 1103 18-19 0.18394721079646104 1103 20-21 0.11914670305146302 1103 22-23 0.17965684797046322 1103 24-25 0.24118968991705714 1103 26-27 0.27686582016188765 1103 28-29 0.2734998391642307 1103 30-31 0.22120663305700816 1103 32-33 0.23117940111065138 1103 34-35 0.1283650809104202 1103 36-37 0.20017627488265788 1103 38-39 0.4099490742181686 1103 40-41 0.5290101984386553 1103 42-43 0.43459548167071205 1103 44-45 0.47648079625854933 1103 46-47 0.4132062584262286 1103 48-49 0.40440515708821323 1103 50-51 0.4843206876692747 1103 52-53 0.4490156564522465 1103 54-55 0.39354309625912265 1103 56-57 0.3110545899519437 1103 58-59 0.3773128900057472 1103 60-61 0.5753529988117592 1103 62-63 0.489474228740967 1103 64-65 0.5942339642402032 1103 66-67 0.4557872545976096 1103 68-69 0.2616095167958754 1103 70-71 0.059813508082537936 1103 72-73 0.10449790165615624 1103 74-75 0.06956883378748202 1103 76-77 0.2086687279331656 1103 78-79 0.20453965744536973 1103 80-81 0.2789203737668693 1103 82-83 0.2804016693939566 1103 84-85 0.04403188449519746 1103 86-87 0.266449184298704 1103 88-89 0.5409401931599795 1103 90-91 0.7465953901011808 1103 92-93 0.4895054888521173 1103 94-95 0.30288676273303494 1103 96-97 0.3147672271489306 1103 98-99 0.07507385293156332 1103 100-101 -0.4456829294707987 1104 1 0.11003106963905651 1104 2 0.1423453381456099 1104 3 0.08757064591349462 1104 4 0.1307142857589767 1104 5 0.06080138238880295 1104 6 0.07042962383115281 1104 7 0.1666611860098257 1104 8 0.09814572256419751 1104 9 0.1318263768524801 1104 10-11 0.1879428570389834 1104 12-13 0.17842995291784547 1104 14-15 0.212855354673664 1104 16-17 0.16807991753812246 1104 18-19 0.27307995269071483 1104 20-21 0.27880447315037316 1104 22-23 0.3220344973012459 1104 24-25 0.20960649520922203 1104 26-27 0.2629127114933212 1104 28-29 0.2164474701736907 1104 30-31 0.23564119490450253 1104 32-33 0.21712993489133936 1104 34-35 0.32082757841784115 1104 36-37 0.22690244679790794 1104 38-39 0.42802849425277856 1104 40-41 0.531977877171883 1104 42-43 0.47189589105646945 1104 44-45 0.45084500371100233 1104 46-47 0.34273978081664325 1104 48-49 0.3130143696730485 1104 50-51 0.23775844403723312 1104 52-53 0.10972566438836395 1104 54-55 0.3393580335531112 1104 56-57 0.11752044926725347 1104 58-59 0.06965126025161084 1104 60-61 0.06534118162879565 1104 62-63 0.1706361268241281 1104 64-65 0.04577715718148312 1104 66-67 -0.0029462859936799646 1104 68-69 0.2428335119672944 1104 70-71 0.19827060956711406 1104 72-73 -0.005551789397447493 1104 74-75 -0.15020601073559092 1104 76-77 -0.0815843043185076 1104 78-79 0.20273027677014355 1104 80-81 0.4993406131350824 1104 82-83 0.3938288933462992 1104 84-85 0.118859545000781 1104 86-87 0.2015098828641797 1104 88-89 -0.02437215920887681 1104 90-91 0.02810798952009108 1104 92-93 0.11675801388835438 1104 94-95 0.17140822303171532 1104 96-97 -0.10443562824648822 1104 98-99 -0.44261411455311617 1104 100-101 -0.3705715495102204 1105 1 -0.012646491019289385 1105 2 0.05585055310429965 1105 3 0.05298709817435565 1105 4 0.06881522061182466 1105 5 -0.035516671320181104 1105 6 -0.02676281376042766 1105 7 0.05367759752270018 1105 8 -0.014005927859869871 1105 9 0.10039949206218779 1105 10-11 0.06354428165129633 1105 12-13 0.10171207264901483 1105 14-15 0.03481538507792692 1105 16-17 0.09636607288720711 1105 18-19 0.14057063420079885 1105 20-21 0.18133515821284618 1105 22-23 0.1013991682743125 1105 24-25 0.06205372526034125 1105 26-27 0.2241521686047605 1105 28-29 0.21843817911030783 1105 30-31 0.18189172710977175 1105 32-33 0.07708223885041576 1105 34-35 0.17378970891065393 1105 36-37 0.3447761202027948 1105 38-39 0.2007412522907117 1105 40-41 0.29154549140337593 1105 42-43 0.42175758298601806 1105 44-45 0.3812599801633638 1105 46-47 0.34145824357272403 1105 48-49 0.37229518076694745 1105 50-51 0.44580434298745075 1105 52-53 0.33659465177477443 1105 54-55 0.3203184714372398 1105 56-57 0.4549823459815556 1105 58-59 0.19966280551452087 1105 60-61 0.15605867270211604 1105 62-63 0.23804661838589425 1105 64-65 0.2980352747812134 1105 66-67 0.30915260101163256 1105 68-69 0.19893702491392062 1105 70-71 0.3823130710589595 1105 72-73 0.1842626505397611 1105 74-75 0.14492891472955094 1105 76-77 -0.13266588910174448 1105 78-79 -0.07609304675058226 1105 80-81 0.2824251704317149 1105 82-83 0.3771421356583815 1105 84-85 0.17295119140229076 1105 86-87 0.19482499279232357 1105 88-89 0.2878909604674398 1105 90-91 0.5183591642897731 1105 92-93 0.5063361938638522 1105 94-95 0.20431940548082395 1105 96-97 0.058446939190123004 1105 98-99 0.41819298291032325 1105 100-101 -0.3927765902451483 1106 1 0.01073218817932542 1106 2 0.09007513880798257 1106 3 0.10511423149119992 1106 4 0.0636037439855599 1106 5 0.044673781314003236 1106 6 0.11986098045179006 1106 7 0.08115471847249012 1106 8 0.0984031742920024 1106 9 0.10066909384304523 1106 10-11 0.14857148917045748 1106 12-13 0.19404202565420547 1106 14-15 0.1804151872050639 1106 16-17 0.13446278325079675 1106 18-19 0.08411132180901149 1106 20-21 0.11212541297415868 1106 22-23 0.22277685810859538 1106 24-25 0.2591722016392666 1106 26-27 0.3222884535416881 1106 28-29 0.29232761764604476 1106 30-31 0.2718459690093553 1106 32-33 0.18410425220683635 1106 34-35 0.2504345271149617 1106 36-37 0.13304473261931804 1106 38-39 0.20146472495951429 1106 40-41 0.12521726978576453 1106 42-43 0.01173779569934652 1106 44-45 0.12978582730656996 1106 46-47 0.0966449860906522 1106 48-49 -0.016960322572153075 1106 50-51 0.10461215896568632 1106 52-53 0.15780414967237277 1106 54-55 -0.07104314122662458 1106 56-57 -0.08074112668726485 1106 58-59 -0.13885933151244245 1106 60-61 -0.11792351873988594 1106 62-63 0.021686242427449542 1106 64-65 0.23186164134069998 1106 66-67 0.21405490128370275 1106 68-69 -0.30579955683230864 1106 70-71 -0.0933584599823476 1106 72-73 0.11515307843983535 1106 74-75 -0.13806391237903526 1106 76-77 -0.1131605712684518 1106 78-79 0.12417531832485551 1106 80-81 0.18412440367435678 1106 82-83 0.1674191576717412 1106 84-85 -0.16229367627023095 1106 86-87 -0.1370288286274146 1106 88-89 -0.170501322490761 1106 90-91 0.23252809824970555 1106 92-93 0.3413930823130258 1106 94-95 -0.26539862568415273 1106 96-97 -0.910903790466076 1106 98-99 -0.5357657111287573 1106 100-101 -0.606576985990884 1107 1 0.10415494434658967 1107 2 0.02105548522426659 1107 3 0.014150797654373548 1107 4 0.03966544574096531 1107 5 0.014488326132337193 1107 6 0.04582762184678302 1107 7 0.0068350124014600055 1107 8 0.07933978127864805 1107 9 -0.02332402448669768 1107 10-11 -0.04808448563562706 1107 12-13 -0.045921816417290984 1107 14-15 -0.05689396308361694 1107 16-17 -0.03178933082163837 1107 18-19 0.001717025430636454 1107 20-21 0.1326179189518797 1107 22-23 0.09873650119484978 1107 24-25 0.016050955940308143 1107 26-27 0.20691491881257917 1107 28-29 0.17760259286036728 1107 30-31 0.14620504056706807 1107 32-33 0.09930749475655176 1107 34-35 0.05086608948738558 1107 36-37 0.12253239810015515 1107 38-39 0.11814402746141894 1107 40-41 0.27603723829336957 1107 42-43 0.07152026493595542 1107 44-45 -0.190760623967698 1107 46-47 0.1589296422987374 1107 48-49 -0.10530434776606512 1107 50-51 -0.29407215899080796 1107 52-53 -0.07921107164914076 1107 54-55 -0.3817184780202325 1107 56-57 -0.2472005656561791 1107 58-59 -0.32851612278788167 1107 60-61 -0.40065723309224666 1107 62-63 -0.2284680119610769 1107 64-65 -0.40417323356452783 1107 66-67 -0.4626716398022026 1107 68-69 -0.32472560803660144 1107 70-71 -0.30814471996951553 1107 72-73 -0.7728366573740075 1107 74-75 -0.464837692048949 1107 76-77 -0.3651909555922437 1107 78-79 -0.2699673888542442 1107 80-81 -0.1594724354228596 1107 82-83 -0.23157104469602174 1107 84-85 -0.3541374390275642 1107 86-87 -0.5630441665874066 1107 88-89 -0.6436893437189646 1107 90-91 -0.0704937131458756 1107 92-93 0.0361551398157971 1107 94-95 -0.08427189797668078 1107 96-97 -0.1786130107378412 1107 98-99 -0.4342584503834388 1107 100-101 -0.6645197748169167 1108 1 -0.0953344898078825 1108 2 -0.06701321801546811 1108 3 -0.09394333346040895 1108 4 0.011208317484175723 1108 5 0.022712497564640444 1108 6 -0.002765951115542009 1108 7 4.981270874253596E-4 1108 8 0.04102614366993862 1108 9 0.04283754349493307 1108 10-11 0.06317725132588947 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13 6471.0 14 7836.0 15 10629.0 16 14980.0 17 22364.0 18 20993.0 19 12234.0 20 10382.0 21 10853.0 22 11213.0 23 11888.0 24 13394.0 25 15140.0 26 17369.0 27 20318.0 28 24014.0 29 28806.0 30 34753.0 31 42027.0 32 52587.0 33 68377.0 34 99563.0 35 155777.0 36 205960.0 37 289005.0 38 167678.0 39 6320.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.45114347963113 20.72542858399785 15.492632704000647 19.330795232370377 2 15.146507637959772 23.46283860209285 39.58474720511213 21.805906554835243 3 19.580122273426824 29.179297254557245 30.494242893656015 20.746337578359917 4 11.882483645767577 18.406438134716215 38.38039775848808 31.330680461028127 5 11.577697775077507 40.59031076811928 33.0256748342673 14.806316622535915 6 27.37758473715091 38.63821615523109 17.815179399232864 16.16901970838513 7 25.576303114000975 36.51283023556271 21.380685739482768 16.530180910953547 8 25.77875159879723 33.447663942696806 20.335120826340276 20.438463632165682 9 27.11028666676721 17.938486972271235 21.902714131105324 33.04851222985623 10-11 24.252200971953812 28.366702502705653 26.219125528832933 21.161970996507602 12-13 26.830097676546355 26.708190509841266 27.401212105543237 19.06049970806914 14-15 22.917424478819743 27.209923871073844 28.759864425086747 21.11278722501967 16-17 19.261826062132723 30.675531313232874 28.994109187405403 21.068533437229 18-19 18.978809338627585 28.47887864078716 33.444539959337945 19.09777206124731 20-21 20.878298944380887 26.573643778281923 33.24582589143045 19.302231385906744 22-23 20.01449959172769 26.924283979423365 33.50375201173756 19.557464417111387 24-25 20.2626660514452 27.27263587088249 33.62804117196098 18.836656905711326 26-27 19.65637883504482 26.93856064199689 34.52438962644144 18.880670896516854 28-29 18.832857434223946 28.134865690163824 34.106561662448684 18.925715213163542 30-31 19.234545079798025 27.47119651435273 33.696146297217936 19.598112108631316 32-33 19.14662049392008 27.919274775719376 33.70647605806161 19.227628672298938 34-35 19.90331672266602 27.520509553408157 33.52398012465901 19.05219359926681 36-37 19.844504722470454 27.13197200136775 33.92240896278055 19.10111431338124 38-39 19.269541132307495 28.099953597487655 33.68265709692666 18.947848173278185 40-41 19.101707855387833 28.103195289051236 33.60393469656282 19.19116215899811 42-43 19.02146726044374 28.402975014175887 32.38637118584358 20.1891865395368 44-45 19.367265608111456 27.838029418249484 32.361115799256105 20.433589174382956 46-47 20.14023500819103 28.489626013644294 31.83728257504972 19.532856403114963 48-49 19.987525212608226 28.595451693953315 31.442425121650725 19.974597971787734 50-51 19.208053190007195 28.97571317153605 31.723619367988036 20.09261427046872 52-53 19.703322122901096 29.637050586267545 30.477689372639503 20.181937918191853 54-55 19.1244156894344 28.936955143189753 30.84588851472904 21.092740652646803 56-57 19.96570798644837 28.937683691185157 30.350730096717843 20.74587822564863 58-59 20.452166519023045 28.507691053658373 30.61932399450026 20.420818432818322 60-61 19.916777475624123 29.24954296915818 31.29821895323329 19.5354606019844 62-63 19.564165118415204 29.155627819259983 31.225425173671084 20.05478188865373 64-65 19.37030267556727 29.571176696602663 30.807841053134304 20.250679574695763 66-67 19.756841306958226 29.12823249240174 30.02810207235727 21.086824128282764 68-69 19.28978796843119 29.071120313533523 30.66191367773471 20.97717804030058 70-71 20.15403779164861 29.11055006982295 30.185525445967382 20.549886692561063 72-73 19.84548131965675 28.67310422685721 30.704770645759677 20.776643807726362 74-75 18.982615571225743 30.25527612063028 29.859643377550267 20.90246493059371 76-77 19.28464886585414 28.920629557878996 30.321787605085053 21.472933971181803 78-79 19.204812111730977 28.954050208405608 30.50213433488695 21.339003344976465 80-81 19.69374069758465 29.320315487657524 29.860587203467276 21.125356611290552 82-83 19.58719612080264 28.74807982746994 30.301633161047448 21.363090890679974 84-85 19.025633181155847 28.840398921902572 30.114301883079715 22.01966601386187 86-87 19.10179967879824 29.04967268764207 29.98657106337337 21.861956570186322 88-89 19.052043403393704 28.72363362090101 29.705134972209486 22.519188003495806 90-91 19.2828331303965 28.12776619369224 29.898166213018413 22.691234462892847 92-93 19.730217106071084 28.64103851261048 29.747395423759365 21.881348957559073 94-95 19.22521594093874 28.021314910203397 30.094742908645 22.65872624021286 96-97 18.66982895287272 27.988854197899034 30.57677012321785 22.7645467260104 98-99 19.047312975465562 28.367174141136744 29.490552988938212 23.094959894459482 100-101 19.176465349897914 26.757137963401174 30.543488486831478 23.522908199869438 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8279.0 1 5389.0 2 3394.5 3 4192.5 4 3399.0 5 3111.0 6 5234.5 7 9736.0 8 13892.5 9 14008.5 10 10077.0 11 6679.0 12 5914.0 13 5851.5 14 5695.0 15 5452.0 16 5258.0 17 5124.0 18 5091.0 19 5200.0 20 5402.0 21 5722.5 22 6264.0 23 6972.0 24 7549.0 25 8324.5 26 9743.0 27 11394.5 28 12536.0 29 14300.5 30 17290.0 31 20097.5 32 23779.0 33 28338.5 34 31970.0 35 34465.5 36 38833.5 37 43304.0 38 44007.5 39 43352.0 40 42176.0 41 40842.5 42 39345.0 43 40303.0 44 42061.0 45 42399.0 46 41612.0 47 47424.0 48 66353.5 49 64626.0 50 45355.0 51 37302.0 52 34373.0 53 32980.5 54 31499.0 55 29181.5 56 26119.5 57 23511.5 58 21316.0 59 19113.0 60 16872.5 61 14452.0 62 12333.0 63 9623.0 64 6952.5 65 5455.0 66 4164.0 67 3359.5 68 2534.5 69 1627.0 70 1312.5 71 1145.5 72 1013.0 73 911.5 74 738.0 75 613.5 76 418.5 77 213.0 78 134.0 79 88.0 80 50.5 81 24.0 82 11.0 83 6.0 84 7.0 85 5.0 86 3.0 87 4.5 88 3.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.42026553140393247 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.976963674896029E-4 16-17 0.002190379136674631 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.590785469958412E-5 26-27 0.0034112461964604913 28-29 7.181570939916824E-5 30-31 0.0 32-33 7.181570939916824E-5 34-35 0.01256774914485444 36-37 0.02764904811867977 38-39 0.02061110859756128 40-41 0.008761516546698524 42-43 0.008869240110797276 44-45 0.003734416888756748 46-47 0.0054579939143367855 48-49 0.0031957990682629864 50-51 7.899728033908506E-4 52-53 0.0 54-55 0.0 56-57 3.590785469958412E-5 58-59 0.0016876691708804536 60-61 0.0029803519400654816 62-63 0.0015799456067817012 64-65 0.0027289969571683927 66-67 0.0020826555725758787 68-69 0.004739836820345103 70-71 1.795392734979206E-4 72-73 0.0 74-75 0.0 76-77 0.09465310498810373 78-79 0.015512193230220338 80-81 3.949864016954253E-4 82-83 0.0 84-85 0.0 86-87 0.10248101731261308 88-89 0.4056869423959013 90-91 0.4116835541307319 92-93 0.0 94-95 3.590785469958412E-5 96-97 3.590785469958412E-5 98-99 1.4363141879833647E-4 100-101 2.8726283759667294E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1392453.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.139193911485876 #Duplication Level Percentage of deduplicated Percentage of total 1 69.61867627867657 25.159628418960544 2 14.395510988087109 10.404843261068113 3 5.8884392744237175 6.384103463232238 4 2.8836594475750332 4.168525118024094 5 1.7507359636199975 3.1635093238537584 6 1.0845206148035165 2.3516220479632888 7 0.7426292221342287 1.8786615024131366 8 0.5354434630911911 1.5480396113032069 9 0.3828525224062515 1.245238339206691 >10 2.2970560013872383 15.910459374154678 >50 0.2572416826568268 6.535389726322742 >100 0.1503267027847068 9.451939683498937 >500 0.0063546281125427696 1.5247400216668991 >1k 0.005361717469957962 4.678926630547718 >5k 3.971642570339231E-4 1.095507692306562 >10k+ 7.943285140678462E-4 4.498865785477482 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21658 1.5553846341671855 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15994 1.1486204561302968 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12763 0.9165838990615842 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12273 0.8813942014559918 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9277 0.6662343360960837 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5988 0.4300324678822193 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4710 0.3382519912700824 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4693 0.3370311242102965 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4132 0.2967425112373631 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3793 0.2723969857510451 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3761 0.2700988830502717 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3374 0.24230620351279364 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3312 0.23785362953004519 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2890 0.20754740016359616 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2887 0.2073319530353987 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2745 0.1971341223007168 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2734 0.19634414949732593 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2693 0.19339970541196005 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2357 0.16926962705383952 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2334 0.16761786573765866 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2238 0.1607235576353385 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1995 0.1432723402513406 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1896 0.13616258502082296 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1579 0.11339700514128663 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1471 0.10564090852617646 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1457 0.10463548859458811 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1450 0.10413277862879394 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 7.181570939916824E-5 6 0.0 0.0 0.0 0.0 7.181570939916824E-5 7 0.0 0.0 0.0 0.0 7.181570939916824E-5 8 0.0 0.0 0.0 0.0 7.181570939916824E-5 9 0.0 0.0 0.0 0.0 7.181570939916824E-5 10-11 0.0 0.0 0.0 0.0 7.181570939916824E-5 12-13 0.0 0.0 0.0 0.0 7.181570939916824E-5 14-15 0.0 0.0 0.0 0.0 7.181570939916824E-5 16-17 0.0 0.0 0.0 0.0 7.181570939916824E-5 18-19 0.0 0.0 0.0 1.0772356409875235E-4 7.181570939916824E-5 20-21 0.0 0.0 0.0 2.1544712819750468E-4 7.181570939916824E-5 22-23 0.0 0.0 0.0 6.822492392920982E-4 7.181570939916824E-5 24-25 0.0 0.0 0.0 0.002154471281975047 7.181570939916824E-5 26-27 0.0 0.0 0.0 0.004237126854550926 7.181570939916824E-5 28-29 0.0 0.0 0.0 0.009659212914188127 7.181570939916824E-5 30-31 0.0 0.0 0.0 0.023268289845330508 7.181570939916824E-5 32-33 0.0 0.0 0.0 0.051886850040899046 7.181570939916824E-5 34-35 0.0 0.0 0.0 0.08994917602245821 7.181570939916824E-5 36-37 0.0 0.0 0.0 0.14664767859310152 7.181570939916824E-5 38-39 0.0 0.0 0.0 0.23293425343620217 7.181570939916824E-5 40-41 0.0 0.0 0.0 0.33010090825327676 7.181570939916824E-5 42-43 0.0 0.0 0.0 0.4312892427967048 7.181570939916824E-5 44-45 0.0 0.0 0.0 0.5446862479379915 7.181570939916824E-5 46-47 0.0 0.0 0.0 0.6827160414031928 7.181570939916824E-5 48-49 0.0 0.0 0.0 0.8268501701673234 7.181570939916824E-5 50-51 0.0 0.0 0.0 0.9900513697769332 7.181570939916824E-5 52-53 0.0 0.0 0.0 1.1522112416002552 7.181570939916824E-5 54-55 0.0 0.0 0.0 1.3271902175513284 7.181570939916824E-5 56-57 0.0 0.0 3.590785469958412E-5 1.547484906133277 7.181570939916824E-5 58-59 0.0 0.0 7.181570939916824E-5 1.7896833860819719 7.181570939916824E-5 60-61 0.0 0.0 7.181570939916824E-5 2.0634807781663005 7.181570939916824E-5 62-63 0.0 0.0 7.181570939916824E-5 2.3288039165415277 7.181570939916824E-5 64-65 0.0 0.0 7.181570939916824E-5 2.6024576772070582 7.181570939916824E-5 66-67 0.0 0.0 7.181570939916824E-5 2.923330266802542 7.181570939916824E-5 68-69 0.0 0.0 7.181570939916824E-5 3.2889081354990077 7.181570939916824E-5 70-71 0.0 0.0 7.181570939916824E-5 3.688921636852375 7.181570939916824E-5 72-73 0.0 0.0 7.181570939916824E-5 4.09004828170143 7.181570939916824E-5 74-75 0.0 0.0 7.181570939916824E-5 4.5103856288147615 7.181570939916824E-5 76-77 0.0 0.0 7.181570939916824E-5 4.954098989337522 7.181570939916824E-5 78-79 0.0 0.0 7.181570939916824E-5 5.481657190583811 7.181570939916824E-5 80-81 0.0 0.0 7.181570939916824E-5 6.058085982076236 7.181570939916824E-5 82-83 0.0 0.0 7.181570939916824E-5 6.625717349167261 7.181570939916824E-5 84-85 0.0 0.0 7.181570939916824E-5 7.198555355189726 7.181570939916824E-5 86-87 0.0 0.0 7.181570939916824E-5 7.796313412373703 7.181570939916824E-5 88-89 0.0 0.0 7.181570939916824E-5 8.423013200445546 7.181570939916824E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 260 0.0 31.941923 94-95 ATGGGCG 180 2.1282176E-10 29.000195 5 GTACATG 12545 0.0 24.844593 1 TACATGG 12645 0.0 24.431093 2 ACATGGG 12500 0.0 23.689468 3 CATGGGG 2965 0.0 23.207268 4 GAGTACT 12985 0.0 23.0804 12-13 GGACCGT 105 0.002208622 22.597553 6 GTACTTT 13725 0.0 21.784124 14-15 CATGGGA 8800 0.0 21.46254 4 AGTACTT 13480 0.0 21.28229 12-13 CGGATTA 185 6.605809E-6 20.521759 9 CGGTATA 105 8.668776E-7 20.344372 34-35 CTATACA 490 0.0 20.3378 4 ATGGGAG 2535 0.0 20.217457 5 TAGTACT 190 8.321389E-6 19.980997 4 ACTTTTT 15065 0.0 19.955656 16-17 ATGGGAT 3405 0.0 19.23281 5 GTATCAA 28525 0.0 19.09613 1 ACTATAC 475 0.0 18.981945 3 >>END_MODULE