FastQCFastQC Report
Fri 27 May 2016
SRR939077_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939077_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2061647
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63150.30630849995173764No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47620.23098037636899044No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30310.1470183789950462No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA22320.10826295675253814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41200.046.7805861
GTATCAA69450.039.1668971
ATCAACG87600.030.9374283
TATCAAC87900.030.8857422
TCAACGC88300.030.6921734
CAACGCA89600.030.2468625
AACGCAG92750.029.2614546
ACGCAGA106400.025.3294547
CGCAGAG108400.024.7310378
TAAGGTG12000.024.084685
TACATGG79300.023.8989662
GTACATG81050.023.721321
GTAAGGT11950.022.9960024
ACATGGG80550.022.645793
AGGTAAG11250.021.899952
GCAGAGT123350.021.5795219
GAGTACT64950.020.96642912-13
GGTAAGG12500.020.468033
CATGGGG51550.020.4040584
AGAGTAC103750.020.06390410-11
Produced by FastQC (version 0.11.5)