##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939077_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2061647 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2201696022646 34.0 31.0 34.0 31.0 34.0 2 32.3274236569112 34.0 31.0 34.0 31.0 34.0 3 32.39372162159671 34.0 31.0 34.0 30.0 34.0 4 35.82058422222621 37.0 35.0 37.0 35.0 37.0 5 35.85648658572491 37.0 35.0 37.0 35.0 37.0 6 35.93338384311184 37.0 37.0 37.0 35.0 37.0 7 35.85889000396285 37.0 37.0 37.0 35.0 37.0 8 35.84486626469032 37.0 37.0 37.0 35.0 37.0 9 37.55510618452141 39.0 38.0 39.0 35.0 39.0 10-11 37.47419999641064 39.0 38.0 39.0 35.0 39.0 12-13 37.38975901306092 39.0 38.0 39.0 35.0 39.0 14-15 38.75535870107734 40.5 38.5 41.0 35.0 41.0 16-17 38.69174111765982 40.0 38.0 41.0 35.0 41.0 18-19 38.61347723446352 40.0 38.0 41.0 34.0 41.0 20-21 38.50354692146618 40.0 38.0 41.0 34.0 41.0 22-23 38.42761030380079 40.0 38.0 41.0 34.0 41.0 24-25 38.457709782518535 40.0 38.0 41.0 34.0 41.0 26-27 38.38018656928175 40.0 38.0 41.0 34.0 41.0 28-29 38.26251560039134 40.0 38.0 41.0 34.0 41.0 30-31 38.087494124842905 40.0 38.0 41.0 33.5 41.0 32-33 37.88128253769923 40.0 38.0 41.0 33.0 41.0 34-35 37.58376652259092 40.0 37.5 41.0 32.5 41.0 36-37 37.33698130669315 40.0 37.0 41.0 32.0 41.0 38-39 37.090595528720485 40.0 37.0 41.0 31.0 41.0 40-41 36.923105895432144 40.0 37.0 41.0 31.0 41.0 42-43 36.72083169427162 40.0 36.0 41.0 30.5 41.0 44-45 36.46146551761771 40.0 36.0 41.0 30.0 41.0 46-47 36.11215838598945 39.0 35.5 41.0 29.5 41.0 48-49 36.09444560586754 39.5 36.0 41.0 29.0 41.0 50-51 35.07847924499199 38.0 34.0 40.0 27.5 40.5 52-53 35.21735850026702 38.5 35.0 40.0 27.5 40.5 54-55 35.68524776550011 39.0 35.0 41.0 28.0 41.0 56-57 35.53440550200883 39.0 35.0 41.0 28.0 41.0 58-59 35.313430718255844 39.0 35.0 41.0 27.5 41.0 60-61 34.99962141918573 38.0 35.0 40.5 27.0 41.0 62-63 34.61581662622166 37.5 34.5 40.0 26.0 41.0 64-65 34.22983954091074 37.0 34.0 40.0 26.0 41.0 66-67 33.83292265843765 36.0 34.0 39.0 25.5 41.0 68-69 33.42689291619759 36.0 34.0 39.0 24.5 41.0 70-71 33.011521128495815 35.0 33.0 38.5 23.5 40.5 72-73 32.577175190515156 35.0 33.0 37.5 22.0 40.0 74-75 32.14511383374554 35.0 33.0 37.0 20.5 39.0 76-77 31.736814546816213 35.0 32.0 36.5 20.0 39.0 78-79 31.34560935989527 35.0 32.0 36.0 18.0 38.5 80-81 30.969904401674974 35.0 31.5 35.5 16.0 37.0 82-83 30.58129713767682 35.0 31.0 35.0 11.0 37.0 84-85 30.246840026444875 34.5 31.0 35.0 7.0 36.5 86-87 29.904029399795405 34.0 31.0 35.0 3.5 36.0 88-89 29.539381620616915 34.0 30.0 35.0 2.0 36.0 90-91 29.202710502816437 34.0 30.0 35.0 2.0 35.0 92-93 28.836489709441043 34.0 29.5 35.0 2.0 35.0 94-95 28.41188379969995 34.0 29.0 35.0 2.0 35.0 96-97 27.887732235440886 34.0 29.0 35.0 2.0 35.0 98-99 27.241744343236256 34.0 27.0 35.0 2.0 35.0 100-101 24.75933998400308 31.5 21.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17764400689018345 1101 2 0.27224037448617366 1101 3 0.20589513448209829 1101 4 0.14389914658546132 1101 5 0.25788687838985425 1101 6 0.2891173441570132 1101 7 0.24617784536052056 1101 8 0.27309625360776124 1101 9 0.3550854928099838 1101 10-11 0.2913021071400195 1101 12-13 0.2642178221957039 1101 14-15 0.36213887811001655 1101 16-17 0.3807906725708605 1101 18-19 0.3216961816879973 1101 20-21 0.4501473252124626 1101 22-23 0.3653820326874495 1101 24-25 0.38947782917958307 1101 26-27 0.4127093010750258 1101 28-29 0.37230593942083345 1101 30-31 0.39696282830708185 1101 32-33 0.3126390893888882 1101 34-35 0.46148591566010566 1101 36-37 0.33207708773474565 1101 38-39 0.40570273629260356 1101 40-41 0.2829847558931178 1101 42-43 0.2683376114591951 1101 44-45 0.30285298700503205 1101 46-47 0.3253776957131791 1101 48-49 0.24988311072733893 1101 50-51 0.19329562316743676 1101 52-53 0.3266651077558578 1101 54-55 0.4074004648708822 1101 56-57 0.34032690390225895 1101 58-59 0.395307701907079 1101 60-61 0.27416233106051635 1101 62-63 0.3579322348065972 1101 64-65 0.28679862396074185 1101 66-67 0.1975475722065596 1101 68-69 0.1252020626697643 1101 70-71 -0.002084691637278979 1101 72-73 0.013280298520591316 1101 74-75 -0.19483454679927803 1101 76-77 -0.2387133163259385 1101 78-79 -0.3510926870023674 1101 80-81 -0.22707457111370033 1101 82-83 -0.16535833125278998 1101 84-85 -0.19120309855021844 1101 86-87 -0.13597975292488584 1101 88-89 -0.053017195055442556 1101 90-91 -0.15805462431071504 1101 92-93 -0.14993078113637992 1101 94-95 -0.3205438730768435 1101 96-97 -0.4135202174361723 1101 98-99 -0.28115750118344707 1101 100-101 -0.4667311304623212 1102 1 0.020912590090361505 1102 2 0.06098063005331511 1102 3 0.09708263108905868 1102 4 0.17261076125981134 1102 5 0.236348105341051 1102 6 0.12190118905014202 1102 7 0.15633817273531747 1102 8 0.14424803713425405 1102 9 0.17235571640556202 1102 10-11 0.20190950828711607 1102 12-13 0.101807820185563 1102 14-15 0.11650345965512798 1102 16-17 0.2613805986650277 1102 18-19 0.2720580810839266 1102 20-21 0.3552708463989518 1102 22-23 0.21262351268211432 1102 24-25 0.2749905554492429 1102 26-27 0.21469596956109172 1102 28-29 0.21015490859812758 1102 30-31 0.28696067143656023 1102 32-33 0.33375661242117616 1102 34-35 0.1472201980164698 1102 36-37 0.09382388874362846 1102 38-39 0.027699440829216826 1102 40-41 -0.10022994956200648 1102 42-43 0.09925657035100244 1102 44-45 0.0823032931485912 1102 46-47 0.10584026373896904 1102 48-49 0.08066003896224316 1102 50-51 0.16843365554397138 1102 52-53 0.05141937857956691 1102 54-55 0.14169353221952719 1102 56-57 0.1741127398656559 1102 58-59 0.06766498530665643 1102 60-61 0.03384792683363713 1102 62-63 -0.1306762609156067 1102 64-65 -0.1768312570592343 1102 66-67 -0.13572208209257752 1102 68-69 -0.10479413712980801 1102 70-71 -0.1093518780070255 1102 72-73 -0.22369418438071165 1102 74-75 -0.3010740650688568 1102 76-77 -0.32565295182949683 1102 78-79 -0.4282920713405538 1102 80-81 -0.4075322971852735 1102 82-83 -0.6567387723988354 1102 84-85 -0.6005848983779636 1102 86-87 -0.6616594239067588 1102 88-89 -0.6649043393735532 1102 90-91 -0.7319579583630045 1102 92-93 -0.91094351254144 1102 94-95 -0.9185080514192236 1102 96-97 -1.29751198317949 1102 98-99 -1.5269596002439627 1102 100-101 -1.682090983032154 1103 1 0.2930412853897906 1103 2 0.23523818967672838 1103 3 0.2954087245371042 1103 4 0.23903544109912644 1103 5 0.27241541092055144 1103 6 0.23676887099099986 1103 7 0.33481430365046805 1103 8 0.3356799005173414 1103 9 0.3604144130008393 1103 10-11 0.3653820333450071 1103 12-13 0.35043843954788656 1103 14-15 0.5231824651146226 1103 16-17 0.5682886324023286 1103 18-19 0.5317629078619248 1103 20-21 0.5857052676870325 1103 22-23 0.5052310827286988 1103 24-25 0.4894348308691292 1103 26-27 0.47579764346765074 1103 28-29 0.4893761561120584 1103 30-31 0.5376739550766843 1103 32-33 0.4530858384577172 1103 34-35 0.41916761447608053 1103 36-37 0.41145357283673434 1103 38-39 0.5329215954416142 1103 40-41 0.5397147794071131 1103 42-43 0.44232360549121097 1103 44-45 0.37169193131771294 1103 46-47 0.3177335359980944 1103 48-49 0.46107741694059 1103 50-51 0.40392853128275163 1103 52-53 0.47291887995711335 1103 54-55 0.4768275405242761 1103 56-57 0.48604291582022796 1103 58-59 0.44586969095038853 1103 60-61 0.41326467942112544 1103 62-63 0.3365356508085 1103 64-65 0.341392970921099 1103 66-67 0.2593857837469926 1103 68-69 0.20729928837825184 1103 70-71 0.16395294651730552 1103 72-73 0.2150162427443476 1103 74-75 0.2575867533480576 1103 76-77 0.1263020989060415 1103 78-79 0.2213684710029149 1103 80-81 0.21503219976946397 1103 82-83 0.15518802306454305 1103 84-85 0.05238458142078528 1103 86-87 -0.1265768272387966 1103 88-89 0.0038297793605615027 1103 90-91 -0.07266555150962262 1103 92-93 -0.13959458387543933 1103 94-95 -0.21366211730499884 1103 96-97 -0.2510227641292957 1103 98-99 -0.6750308891689087 1103 100-101 -0.542589380115917 1104 1 0.17493438562746633 1104 2 0.21358398587410932 1104 3 0.2278789177753282 1104 4 0.15016943046352793 1104 5 0.2576592971763745 1104 6 0.21311954804224342 1104 7 0.2099390491696127 1104 8 0.27050070391624104 1104 9 0.23544745370590903 1104 10-11 0.2462443984445315 1104 12-13 0.28256662777363317 1104 14-15 0.3312067981778739 1104 16-17 0.29307061769287657 1104 18-19 0.29901420170896387 1104 20-21 0.4168749518017023 1104 22-23 0.37476385748981045 1104 24-25 0.38800445154565466 1104 26-27 0.48659817794488447 1104 28-29 0.4513127244316095 1104 30-31 0.3698010104782554 1104 32-33 0.36273083833364694 1104 34-35 0.32775989703047514 1104 36-37 0.3188887564135854 1104 38-39 0.4478113015222931 1104 40-41 0.33007622584017327 1104 42-43 0.325515003148098 1104 44-45 0.30729896863726225 1104 46-47 0.38628194049616127 1104 48-49 0.43341331117581916 1104 50-51 0.4132298269099408 1104 52-53 0.4276691623540003 1104 54-55 0.3672715752340068 1104 56-57 0.30120147883815207 1104 58-59 0.43216939630821827 1104 60-61 0.4527835204448962 1104 62-63 0.4712207580545851 1104 64-65 0.3318869577824941 1104 66-67 0.22909945973986368 1104 68-69 0.329778889451525 1104 70-71 0.3361708112624129 1104 72-73 0.16803734995590247 1104 74-75 0.16867165366492998 1104 76-77 0.13525923176042554 1104 78-79 0.22483599185497027 1104 80-81 0.197824330849091 1104 82-83 0.13867724559112915 1104 84-85 0.24853578431822498 1104 86-87 0.13499052131610156 1104 88-89 0.19154494852358184 1104 90-91 0.14400352465607824 1104 92-93 0.19087854823235872 1104 94-95 0.07709824506927276 1104 96-97 -0.018038996960267895 1104 98-99 -0.06491727769308042 1104 100-101 -0.18673262519325817 1105 1 0.10524774718746244 1105 2 0.16625292753868592 1105 3 0.2461346755185474 1105 4 0.20086843678495825 1105 5 0.2296022565846556 1105 6 0.2381512427106216 1105 7 0.22240716556627405 1105 8 0.18616358454726623 1105 9 0.3328596678937217 1105 10-11 0.31793162609644554 1105 12-13 0.3562234195280567 1105 14-15 0.4228716771441512 1105 16-17 0.41560786704910413 1105 18-19 0.42671726436456936 1105 20-21 0.4706331134605506 1105 22-23 0.43496344175335366 1105 24-25 0.33357394659741146 1105 26-27 0.3871103309045054 1105 28-29 0.44449595103767336 1105 30-31 0.4263286272009452 1105 32-33 0.5114634891322538 1105 34-35 0.3332864766659611 1105 36-37 0.17430670839043216 1105 38-39 0.36757519778812053 1105 40-41 0.40189749787496254 1105 42-43 0.23129042007121114 1105 44-45 0.2728479712065095 1105 46-47 0.4150868983616789 1105 48-49 0.35586737611481567 1105 50-51 0.24407138432800934 1105 52-53 0.32209707922473996 1105 54-55 0.4304515587880431 1105 56-57 0.41854984494187164 1105 58-59 0.36773727115981814 1105 60-61 0.49894729218600276 1105 62-63 0.56899360599256 1105 64-65 0.4400138393426758 1105 66-67 0.30564065856599854 1105 68-69 0.37832642376410064 1105 70-71 0.35736228683518334 1105 72-73 0.35972161298625593 1105 74-75 0.38368109776126147 1105 76-77 0.3221959901244098 1105 78-79 0.34604963579649706 1105 80-81 0.18015473553584727 1105 82-83 0.18636893767672902 1105 84-85 0.3486630933354746 1105 86-87 0.3169301156932889 1105 88-89 0.2657094118329404 1105 90-91 0.24145524945328134 1105 92-93 0.2980850182139747 1105 94-95 0.2665358393875934 1105 96-97 -0.003420867000059502 1105 98-99 0.04831005229926788 1105 100-101 -0.1444822599852884 1106 1 0.16399053550150455 1106 2 0.17348175809184596 1106 3 0.18886039245900577 1106 4 0.20082512412997744 1106 5 0.2096451773632353 1106 6 0.2316914695967469 1106 7 0.15808916199091527 1106 8 0.17139835202792142 1106 9 0.26185114819136146 1106 10-11 0.23755880726244527 1106 12-13 0.3064154131837853 1106 14-15 0.3523361161653398 1106 16-17 0.37588758429996716 1106 18-19 0.2798470161882136 1106 20-21 0.3563932789148936 1106 22-23 0.29949615234694704 1106 24-25 0.23607664461488298 1106 26-27 0.367091376461687 1106 28-29 0.2873584122174222 1106 30-31 0.22990612359542695 1106 32-33 0.14151218493149287 1106 34-35 0.25052063364443455 1106 36-37 0.24224750617859314 1106 38-39 0.4321080888511162 1106 40-41 0.3870974605435791 1106 42-43 0.43404404789278317 1106 44-45 0.3270495049901214 1106 46-47 0.1998191359192134 1106 48-49 0.22421121362636143 1106 50-51 0.2404688284688632 1106 52-53 0.12808538893290233 1106 54-55 0.10226783372440451 1106 56-57 0.20543611364430348 1106 58-59 0.19591558214158766 1106 60-61 0.24850900936777265 1106 62-63 0.17368931654440445 1106 64-65 0.025979404750714252 1106 66-67 0.03385916266032751 1106 68-69 -0.0037577090056899465 1106 70-71 -0.05037569944605025 1106 72-73 -0.10530715641355926 1106 74-75 -0.0756095182942218 1106 76-77 0.025663503312859604 1106 78-79 0.02073795225937758 1106 80-81 0.09280638783628703 1106 82-83 0.13057592163609755 1106 84-85 0.26421359975838143 1106 86-87 0.11305255025637351 1106 88-89 0.14395315586133606 1106 90-91 0.02660050411364523 1106 92-93 0.02482857171825259 1106 94-95 -0.23870035122315159 1106 96-97 0.11429235888007483 1106 98-99 0.19403367527910476 1106 100-101 0.21030097822348282 1107 1 0.11028755263605916 1107 2 0.050018004456546805 1107 3 0.05520838433676545 1107 4 0.08667293873662629 1107 5 0.20308069158583208 1107 6 0.14135212557425092 1107 7 0.04072055955672482 1107 8 0.07955629439373268 1107 9 0.12256712515355161 1107 10-11 0.13029887326184308 1107 12-13 0.20243239154183357 1107 14-15 0.2901735821057372 1107 16-17 0.2770015411152329 1107 18-19 0.19036241646143282 1107 20-21 0.14962040660216758 1107 22-23 0.07442877692639627 1107 24-25 0.1288035702378707 1107 26-27 0.17853342120342575 1107 28-29 0.050366517102425234 1107 30-31 -0.038332470929745455 1107 32-33 0.01440222477410913 1107 34-35 -0.05197678166831565 1107 36-37 0.02458436096751626 1107 38-39 -0.05886162134159889 1107 40-41 -0.15071236102002672 1107 42-43 -0.09101130284370385 1107 44-45 0.1153509749767565 1107 46-47 0.18698793025248506 1107 48-49 0.07954273540591572 1107 50-51 0.04111431846853719 1107 52-53 0.07647680851503935 1107 54-55 0.07425713795387168 1107 56-57 0.04549765027496022 1107 58-59 0.055296075376766396 1107 60-61 -0.12498895883251748 1107 62-63 -0.06522677595392423 1107 64-65 -0.15284252331439774 1107 66-67 -0.16709314273193598 1107 68-69 0.014509645588610454 1107 70-71 -0.007564725000364092 1107 72-73 0.01744962017772167 1107 74-75 0.06812351388482085 1107 76-77 0.10278102538496725 1107 78-79 0.10084305047749709 1107 80-81 0.16815070288796719 1107 82-83 0.1039831468596688 1107 84-85 -0.018679489643286473 1107 86-87 -0.07842430408627266 1107 88-89 0.18756631309709704 1107 90-91 0.25499462615055535 1107 92-93 0.14291344863259425 1107 94-95 -0.10362620726908744 1107 96-97 -0.029642117748650776 1107 98-99 0.06452212538410507 1107 100-101 -0.03780767533421425 1108 1 0.0682179291231364 1108 2 0.18365505561936857 1108 3 0.13722136531881546 1108 4 0.1309250365969703 1108 5 0.1598056050117762 1108 6 0.14911321374771092 1108 7 0.19445895453223727 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-0.8796419953264731 2316 76-77 -1.0722409297574664 2316 78-79 -0.8687971812109296 2316 80-81 -0.8827339166713521 2316 82-83 -0.8940098077597902 2316 84-85 -0.9109907494244247 2316 86-87 -0.783431063309898 2316 88-89 -0.5983715415154585 2316 90-91 -0.7079317723180125 2316 92-93 -0.7690997145853906 2316 94-95 -0.6431676654852438 2316 96-97 -0.6656544718332924 2316 98-99 -0.6724962182607435 2316 100-101 -0.3810020745527076 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6652.0 3 1685.0 4 1685.0 5 1866.0 6 2165.0 7 2434.0 8 2945.0 9 3580.0 10 4419.0 11 5376.0 12 7059.0 13 11030.0 14 9785.0 15 9931.0 16 11768.0 17 15624.0 18 14724.0 19 10614.0 20 10363.0 21 10965.0 22 11562.0 23 12631.0 24 14366.0 25 15846.0 26 18526.0 27 21472.0 28 25433.0 29 30680.0 30 37245.0 31 45969.0 32 58649.0 33 78119.0 34 110905.0 35 172384.0 36 292109.0 37 468170.0 38 424181.0 39 78704.0 40 26.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.8627817124692 18.112720514269277 14.188346402738174 23.836151370523346 2 15.803065155309392 19.406936490848288 39.950984319741124 24.8390140341012 3 19.745804684768675 23.328089189294175 28.738103562133393 28.188002563803753 4 13.637476096455234 15.66678871354497 35.242395783458406 35.45333940654139 5 14.81458456373753 36.29472195455534 33.26044807435268 15.630245407354447 6 33.92076771988011 34.235149166131855 16.821640473067376 15.022442640920659 7 30.03676199279908 31.67804029254235 19.895219581538605 18.38997813311997 8 27.016907233312825 34.459575918444436 19.266030992629336 19.257485855613403 9 26.87269644986271 17.005831452950293 19.783442013647505 36.33803008353949 10-11 25.951398185108275 25.711848012223882 26.22267316050153 22.114080642166318 12-13 26.687791469359972 22.97606298019926 26.960290743569104 23.37585480687166 14-15 23.37638063325434 24.406243650743754 25.12799250991061 27.089383206091306 16-17 23.182102161987142 27.5399841650716 25.96437966352476 23.313534009416493 18-19 23.322825325448036 25.99394377527979 28.121609031222523 22.561621868049652 20-21 23.675195186796415 25.282186004720074 28.241470083901373 22.801148724582138 22-23 23.474529831273653 25.036267678900114 28.333124123961046 23.156078365865188 24-25 23.343645507283682 25.35570929382654 28.31223546724104 22.988409731648744 26-27 22.871350384239094 25.689735096362703 28.162939619085225 23.27597490031298 28-29 22.958494249510423 26.022127820762954 28.119013827156298 22.90036410257033 30-31 23.065338174924662 25.602220670513137 27.939819693130648 23.39262146143156 32-33 22.83474775018864 25.585455929722954 28.048692259211677 23.531104060876736 34-35 23.52743377387569 25.65564313198217 27.77764801089263 23.03927508324951 36-37 23.580982265949107 25.349342695442186 27.76878106151829 23.30089397709042 38-39 23.082967319315525 25.634698396896365 27.881606616977933 23.400727666810173 40-41 23.386203948305912 25.538906366274855 27.911348852277722 23.163540833141507 42-43 23.274464758778386 25.556415974486978 27.6000842116754 23.56903505505923 44-45 23.15155071477726 25.80313749495572 27.801111984045445 23.244199806221573 46-47 23.719048505662194 26.02191961896555 27.09769759192232 23.161334283449936 48-49 23.407262506653773 26.163337668440356 26.97824192236974 23.451157902536135 50-51 23.440613265851216 26.279497432378157 27.057947930110775 23.22194137165985 52-53 23.78902443958002 25.95088980425817 26.982288725409198 23.277797030752605 54-55 23.381495953145922 26.205436174477104 26.979790706429764 23.43327716594721 56-57 23.366672692341236 26.459476039173058 26.85646404645649 23.317387222029218 58-59 23.35976199830618 25.959312797876617 26.97387442312381 23.707050780693393 60-61 23.12639635831699 26.15290257485065 27.30100859192542 23.41969247490694 62-63 23.139844060840687 26.18187834055707 27.438343217699124 23.23993438090312 64-65 22.974090804290107 26.264592293192674 27.197709395660073 23.56360750685714 66-67 23.012396979252408 26.289561514839153 27.025492767799104 23.672548738109334 68-69 23.301153585691072 25.9799335277997 27.28168484649329 23.437228040015935 70-71 23.274983290603103 26.120041477330062 26.849989835421585 23.75498539664525 72-73 23.167970393292084 26.17777771278237 26.96466898407791 23.689582909847633 74-75 23.066108055563408 26.567120595063177 26.781499143460806 23.58527220591261 76-77 23.342645781568176 26.231202485312423 26.836714700833543 23.58943703228586 78-79 23.301238647920215 26.335022125500146 26.871652607475937 23.492086619103706 80-81 23.381445800804688 26.351864174359797 26.7101310534591 23.556558971376415 82-83 23.40777264230508 26.3961726256372 26.739180071842465 23.456874660215256 84-85 23.145588596515168 26.543620824772013 26.691559479575645 23.619231099137174 86-87 23.38950164966557 26.43251869901225 26.62252449641884 23.555455154903342 88-89 23.253178393383532 26.435824664064565 26.491343729550497 23.819653213001406 90-91 23.16191642496404 26.422543560708085 26.591148357839977 23.824391656487897 92-93 23.236414094599176 26.136435372942575 26.75750463772828 23.869645894729967 94-95 23.22078115809946 25.88033786371134 26.968111205549842 23.93076977263936 96-97 23.436326205682523 25.663564741893826 26.8980459775158 24.002063074907852 98-99 23.1344158472986 25.96134839698288 26.652793407130687 24.251442348587826 100-101 23.433842536529397 25.36702426974715 27.08674569661439 24.11238749710906 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1794.0 1 1022.0 2 287.5 3 359.0 4 330.0 5 307.0 6 532.5 7 1068.0 8 1778.0 9 2178.5 10 2067.5 11 1923.5 12 2065.0 13 2204.5 14 2375.5 15 2578.5 16 2731.0 17 2835.5 18 2979.0 19 3280.0 20 3568.0 21 4017.0 22 4751.5 23 5738.5 24 6851.5 25 8387.0 26 10592.5 27 13365.5 28 15472.5 29 17410.0 30 19852.0 31 23299.0 32 29152.5 33 31446.0 34 31146.0 35 33051.5 36 36196.0 37 39752.0 38 42609.5 39 45172.0 40 48425.0 41 52584.0 42 55369.0 43 57823.0 44 60629.5 45 64744.5 46 68193.5 47 71287.0 48 75155.5 49 76489.0 50 75955.0 51 75825.5 52 77978.0 53 81317.5 54 83650.5 55 80489.5 56 74531.0 57 69169.0 58 64761.0 59 58364.0 60 48681.0 61 41268.5 62 34958.5 63 26406.0 64 18781.5 65 14350.5 66 11018.5 67 8853.5 68 6984.0 69 5434.5 70 4303.5 71 3015.0 72 2231.5 73 1801.5 74 1366.5 75 1086.0 76 812.0 77 535.0 78 379.0 79 274.0 80 191.5 81 139.0 82 97.0 83 68.5 84 54.0 85 40.0 86 26.0 87 16.5 88 18.5 89 21.5 90 17.5 91 10.0 92 7.0 93 5.5 94 5.0 95 5.5 96 4.0 97 2.0 98 3.0 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3321616164163894 2 0.11185232001404702 3 0.10705033402905542 4 0.11296793291965114 5 0.13110876886295278 6 0.08386498755606561 7 0.09269288098301988 8 0.09681579824286117 9 0.0864842526387883 10-11 0.08461681364462491 12-13 0.08852145881423928 14-15 0.09014637326370616 16-17 0.07974207029622433 18-19 0.09448756261377433 20-21 0.10406728212928788 22-23 0.11187657246851668 24-25 0.12572472397068946 26-27 0.12502140279106946 28-29 0.12002539717032062 30-31 0.11005763838329258 32-33 0.10384901003906101 34-35 0.32401279171458547 36-37 0.483715204397261 38-39 0.45941424501866707 40-41 0.43627740345461663 42-43 0.46928499398781653 44-45 0.4762697008750771 46-47 0.4835454372159735 48-49 0.4937314680932284 50-51 0.48228430958355145 52-53 0.48582516793612096 54-55 0.47265608515909857 56-57 0.45196874149648314 58-59 0.43113588310704987 60-61 0.3924532182279508 62-63 0.46082088737790705 64-65 0.4916457570088381 66-67 0.4996248145293545 68-69 0.5124058580348624 70-71 0.5047420824224516 72-73 0.49572016935974006 74-75 0.5029474007916971 76-77 0.48082916231537215 78-79 0.46031158583404436 80-81 0.463003608280176 82-83 0.4751783404239426 84-85 0.4745235241532619 86-87 0.4786221889586335 88-89 0.48987532783255333 90-91 0.5044753054232853 92-93 0.49792714271647864 94-95 0.5018560403405626 96-97 0.5015407584324572 98-99 0.49564741199633106 100-101 0.48395772894195754 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2061647.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.0064028536273 #Duplication Level Percentage of deduplicated Percentage of total 1 75.43448382559214 40.73945122540361 2 13.502289312250875 14.584201520872941 3 4.654015486674669 7.540399057811174 4 2.0752863626431783 4.483150053501854 5 1.159138013901042 3.130043727084656 6 0.6929868414562338 2.2455435919168867 7 0.4746734575477617 1.7944784180573998 8 0.322116510768765 1.391708323710616 9 0.25024402680795543 1.2163301751153917 >10 1.3038735020702106 12.668986459994327 >50 0.08503690385018751 3.103178341822217 >100 0.04073282884156218 3.9444522249948184 >500 0.0037747887545559124 1.5296502587847145 >1k 0.0012582629181852962 1.3219043963870898 >5k 8.987592272752117E-5 0.3065222245422807 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6315 0.30630849995173764 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4762 0.23098037636899044 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3031 0.1470183789950462 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2232 0.10826295675253814 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.70098178786184E-5 2 0.0 0.0 0.0 0.0 9.70098178786184E-5 3 0.0 0.0 0.0 0.0 9.70098178786184E-5 4 0.0 0.0 0.0 0.0 9.70098178786184E-5 5 0.0 0.0 0.0 0.0 1.455147268179276E-4 6 0.0 0.0 0.0 0.0 1.455147268179276E-4 7 0.0 0.0 0.0 0.0 1.455147268179276E-4 8 0.0 0.0 0.0 0.0 1.455147268179276E-4 9 0.0 0.0 0.0 0.0 1.455147268179276E-4 10-11 0.0 0.0 0.0 0.0 1.455147268179276E-4 12-13 0.0 0.0 0.0 0.0 3.395343625751644E-4 14-15 0.0 0.0 0.0 0.0 5.335539983324012E-4 16-17 0.0 0.0 0.0 0.0 7.760785430289473E-4 18-19 0.0 0.0 0.0 0.0 8.730883609075656E-4 20-21 0.0 0.0 0.0 0.0 0.0010428555421951478 22-23 0.0 0.0 0.0 2.42524544696546E-5 0.0011398653600737663 24-25 0.0 0.0 0.0 9.70098178786184E-5 0.0011641178145434209 26-27 0.0 0.0 0.0 3.63786817044819E-4 0.0013096325413613484 28-29 0.0 0.0 0.0 0.0019159439031027135 0.0013581374503006576 30-31 0.0 0.0 0.0 0.006645172524685361 0.0013581374503006576 32-33 0.0 0.0 0.0 0.016855455856409948 0.001455147268179276 34-35 0.0 0.0 0.0 0.03218300708123166 0.0015521570860578945 36-37 0.0 0.0 0.0 0.0574298121841421 0.0015521570860578945 38-39 0.0 0.0 0.0 0.09346895952604883 0.0015521570860578945 40-41 0.0 0.0 0.0 0.13535294839514234 0.0016006619949972037 42-43 0.0 0.0 0.0 0.1801229793461247 0.0016006619949972037 44-45 0.0 0.0 0.0 0.23556409026375513 0.0016976718128758222 46-47 0.0 0.0 0.0 0.30286465141704666 0.001818934085224095 48-49 0.0 0.0 0.0 0.37571902464388907 0.0019401963575723681 50-51 0.0 0.0 0.0 0.45701325202617127 0.0020857110843902956 52-53 0.0 0.0 0.0 0.5384529941352715 0.0022069733567385685 54-55 0.0 0.0 0.0 0.6315339143898058 0.0022312258112082233 56-57 0.0 0.0 0.0 0.7416158052275681 0.0022312258112082233 58-59 0.0 0.0 0.0 0.869693017281814 0.0022312258112082233 60-61 0.0 0.0 0.0 1.0088536010286921 0.0022312258112082233 62-63 0.0 0.0 0.0 1.1539802885751054 0.0024737503559047695 64-65 0.0 0.0 0.0 1.306212945281127 0.0025465077193137335 66-67 0.0 0.0 0.0 1.494751526328222 0.0026435175371923515 68-69 0.0 0.0 0.0 1.7015764580454364 0.002667769991662006 70-71 0.0 0.0 0.0 1.9308834150560208 0.0027405273550709696 72-73 0.0 0.0 0.0 2.1667142823189423 0.0029587994452978613 74-75 0.0 0.0 0.0 2.41750891398964 0.0032013239899944075 76-77 0.0 0.0 0.0 2.695296527485064 0.003346838716812335 78-79 0.0 0.0 0.0 3.0141192939431436 0.003443848534690953 80-81 0.0 0.0 0.0 3.3653918444816204 0.003565110807039226 82-83 0.0 0.0 0.0 3.7268019209884136 0.0038076353517357726 84-85 0.0 0.0 0.0 4.103879083082603 0.003904645169614391 86-87 0.0 0.0 0.0 4.506591089551218 0.004050159896432318 88-89 0.0 0.0 0.0 4.943159522459471 0.0041956746232502465 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4120 0.0 46.780586 1 GTATCAA 6945 0.0 39.166897 1 ATCAACG 8760 0.0 30.937428 3 TATCAAC 8790 0.0 30.885742 2 TCAACGC 8830 0.0 30.692173 4 CAACGCA 8960 0.0 30.246862 5 AACGCAG 9275 0.0 29.261454 6 ACGCAGA 10640 0.0 25.329454 7 CGCAGAG 10840 0.0 24.731037 8 TAAGGTG 1200 0.0 24.08468 5 TACATGG 7930 0.0 23.898966 2 GTACATG 8105 0.0 23.72132 1 GTAAGGT 1195 0.0 22.996002 4 ACATGGG 8055 0.0 22.64579 3 AGGTAAG 1125 0.0 21.89995 2 GCAGAGT 12335 0.0 21.579521 9 GAGTACT 6495 0.0 20.966429 12-13 GGTAAGG 1250 0.0 20.46803 3 CATGGGG 5155 0.0 20.404058 4 AGAGTAC 10375 0.0 20.063904 10-11 >>END_MODULE