FastQCFastQC Report
Fri 27 May 2016
SRR939077_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939077_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2061647
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61250.2970925672532688No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48190.23374515617853106No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32460.15744693441699767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41200.043.6718331
GTATCAA69550.038.0526161
TATCAAC91000.028.9892582
TCAACGC90800.028.9486064
ATCAACG91150.028.9415533
CAACGCA92400.028.447335
AACGCAG95350.027.6169686
ACGCAGA109750.023.906967
TAAGGTG11750.023.4203745
CGCAGAG112000.023.3843218
GTAAGGT11100.022.2271654
GTACATG78550.021.8154011
TACATGG77000.021.443332
ACATGGG74900.020.714273
AGAGTAC105900.020.43119210-11
GAGTACT70500.020.29191412-13
GCAGAGT128750.020.1583219
TGTACGC1200.004795950419.7693542
GGTAAGG12350.019.5932713
AGTACTT72600.019.50888612-13
Produced by FastQC (version 0.11.5)