##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939077_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2061647 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.541327880088105 34.0 31.0 34.0 31.0 34.0 2 32.750823977140605 34.0 31.0 34.0 31.0 34.0 3 32.83835011522341 34.0 31.0 34.0 31.0 34.0 4 36.289177536212556 37.0 37.0 37.0 35.0 37.0 5 36.24668869112899 37.0 37.0 37.0 35.0 37.0 6 36.281058299505204 37.0 37.0 37.0 35.0 37.0 7 36.23430684302405 37.0 37.0 37.0 35.0 37.0 8 36.215125576784 37.0 37.0 37.0 35.0 37.0 9 37.96915766860185 39.0 38.0 39.0 35.0 39.0 10-11 37.91133205636076 39.0 38.0 39.0 35.0 39.0 12-13 37.85033446559959 39.0 38.0 39.0 35.0 39.0 14-15 39.23959678839297 41.0 39.0 41.0 36.0 41.0 16-17 39.17535252155194 40.0 39.0 41.0 36.0 41.0 18-19 39.18908523137084 40.5 38.5 41.0 36.0 41.0 20-21 39.24071628169129 41.0 39.0 41.0 36.0 41.0 22-23 39.20422773636806 41.0 39.0 41.0 36.0 41.0 24-25 39.146694366203334 41.0 39.0 41.0 35.5 41.0 26-27 39.012076509703164 40.5 39.0 41.0 35.0 41.0 28-29 38.864221178504366 40.0 38.0 41.0 35.0 41.0 30-31 38.66377003434633 40.0 38.0 41.0 35.0 41.0 32-33 38.52413143472185 40.0 38.0 41.0 34.0 41.0 34-35 38.487463663760096 40.0 38.0 41.0 34.0 41.0 36-37 38.380276545887824 40.0 38.0 41.0 34.0 41.0 38-39 38.23830000965248 40.0 38.0 41.0 34.0 41.0 40-41 38.05693724483386 40.0 38.0 41.0 33.0 41.0 42-43 37.88620166303931 40.0 37.5 41.0 33.0 41.0 44-45 37.668491744707026 40.0 37.0 41.0 33.0 41.0 46-47 37.38271003716931 40.0 37.0 41.0 32.5 41.0 48-49 37.0615316298086 40.0 36.5 41.0 31.5 41.0 50-51 36.789189177390696 40.0 36.0 41.0 31.0 41.0 52-53 36.52935759613552 39.0 35.5 41.0 31.0 41.0 54-55 36.25518893389606 39.0 35.0 41.0 30.0 41.0 56-57 35.955204018922736 39.0 35.0 40.0 30.0 41.0 58-59 35.62101295711632 38.0 35.0 40.0 29.0 41.0 60-61 35.24066462396327 38.0 35.0 40.0 28.0 41.0 62-63 34.8189770120685 37.0 34.0 40.0 28.0 41.0 64-65 34.38981333855893 36.5 34.0 39.5 27.0 41.0 66-67 34.01853348318116 36.0 34.0 39.0 26.5 40.5 68-69 33.54454666584532 35.5 33.0 39.0 26.0 40.0 70-71 33.10157485738344 35.0 33.0 38.0 25.5 40.0 72-73 32.64832340357006 35.0 32.5 37.0 25.0 39.5 74-75 32.17832611499446 35.0 32.0 37.0 23.5 39.0 76-77 30.247686922154955 33.5 29.5 35.0 20.0 37.0 78-79 31.22645438331586 34.5 31.0 35.5 21.0 37.0 80-81 31.288845762635408 35.0 32.0 35.0 21.5 37.0 82-83 31.02925185543403 34.5 31.0 35.0 20.0 37.0 84-85 30.686639856386666 34.0 31.0 35.0 19.0 36.0 86-87 30.170088283784757 34.0 31.0 35.0 15.5 36.0 88-89 29.645183923338962 34.0 30.0 35.0 7.0 35.5 90-91 29.220196765013604 34.0 29.5 35.0 4.0 35.0 92-93 28.962398994590245 34.0 29.0 35.0 2.0 35.0 94-95 28.651108555441354 34.0 29.0 35.0 2.0 35.0 96-97 28.119606557281628 34.0 29.0 35.0 2.0 35.0 98-99 27.367488711695067 33.5 28.0 35.0 2.0 35.0 100-101 23.740979420822285 30.0 13.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18123991652979043 1101 2 0.22151877359409156 1101 3 0.21792278123279374 1101 4 0.16453673078242304 1101 5 0.20067273779178407 1101 6 0.16263634337916955 1101 7 0.16738381755664733 1101 8 0.1730108645942181 1101 9 0.3052205421044931 1101 10-11 0.26199367604284873 1101 12-13 0.2599917575740349 1101 14-15 0.3472042378347169 1101 16-17 0.3487021857835799 1101 18-19 0.31301069204107534 1101 20-21 0.25114093320944164 1101 22-23 0.3249748132416457 1101 24-25 0.31359507776866025 1101 26-27 0.35521944392922933 1101 28-29 0.18942370693285682 1101 30-31 0.25584405438972624 1101 32-33 0.2840395012491257 1101 34-35 0.3218780582903662 1101 36-37 0.48689160987384383 1101 38-39 0.4362555777559507 1101 40-41 0.3431530515996357 1101 42-43 0.3595310352137986 1101 44-45 0.47986025275399413 1101 46-47 0.46425455636045143 1101 48-49 0.5124296171633063 1101 50-51 0.3935637254059472 1101 52-53 0.33549327283758856 1101 54-55 0.41573357478520023 1101 56-57 0.2875848016479239 1101 58-59 0.35493455524186146 1101 60-61 0.4130191939258836 1101 62-63 0.2951960942224403 1101 64-65 0.17036969427886106 1101 66-67 0.168668390253778 1101 68-69 0.19471699673647436 1101 70-71 0.07450020319362949 1101 72-73 0.0818975969556135 1101 74-75 0.19981123907891885 1101 76-77 0.14983211185125356 1101 78-79 0.28874436023752637 1101 80-81 0.15539245928060907 1101 82-83 0.2665570155041621 1101 84-85 0.3055378017860484 1101 86-87 0.2651560675801896 1101 88-89 0.3235168671911275 1101 90-91 0.5020812500836271 1101 92-93 0.4517303601822853 1101 94-95 0.16179572185961533 1101 96-97 0.1738506239607176 1101 98-99 0.1027490689036803 1101 100-101 -0.1002084666868015 1102 1 0.03623571283317517 1102 2 -0.005604973550255465 1102 3 0.03208711128973363 1102 4 0.03712173275588526 1102 5 -0.003169132787185447 1102 6 0.02274700404181118 1102 7 0.03341486768848512 1102 8 0.059079095065371234 1102 9 0.17220589739389425 1102 10-11 0.12154516435685991 1102 12-13 0.028960553380024123 1102 14-15 0.06683263985777188 1102 16-17 0.11297795415035239 1102 18-19 0.13416915159535847 1102 20-21 0.0684997792397013 1102 22-23 0.17110044287910142 1102 24-25 0.1645224587262888 1102 26-27 0.1437129065574183 1102 28-29 0.0998063240237812 1102 30-31 0.035449596519860904 1102 32-33 -0.05408810038422729 1102 34-35 0.08537735327248441 1102 36-37 0.14097917294763818 1102 38-39 0.07983345912219875 1102 40-41 0.16691266764733825 1102 42-43 0.137581723138112 1102 44-45 0.08329627953440166 1102 46-47 0.023449207321583287 1102 48-49 -0.07420174725537265 1102 50-51 -0.07126534627405334 1102 52-53 0.03265794183133153 1102 54-55 -0.21928519879181607 1102 56-57 -0.11103421432025584 1102 58-59 -0.08734656275069597 1102 60-61 -0.18246076845360193 1102 62-63 -0.19517736717325107 1102 64-65 -0.23037200507099698 1102 66-67 -0.1034762873381041 1102 68-69 -0.07494023692303386 1102 70-71 0.0993589428476227 1102 72-73 -0.01649707513404053 1102 74-75 0.11541113519699309 1102 76-77 0.05434965513171974 1102 78-79 0.21018409331379573 1102 80-81 0.1504759683181902 1102 82-83 0.09047172081255894 1102 84-85 0.05432533243341808 1102 86-87 0.07296261830405726 1102 88-89 0.052240806839837006 1102 90-91 0.15953778620827563 1102 92-93 0.26713085582232665 1102 94-95 -0.08659007331398882 1102 96-97 -0.13636956757525454 1102 98-99 -0.24738291103009047 1102 100-101 -0.5290038618416162 1103 1 0.11393799297448481 1103 2 0.04722956830867986 1103 3 0.07544472781808764 1103 4 9.070548095735376E-5 1103 5 0.09199169408095997 1103 6 0.060863732402438586 1103 7 0.07026813746858096 1103 8 0.06976679965551824 1103 9 0.1494839258914311 1103 10-11 0.11966462528120303 1103 12-13 0.07676619203932233 1103 14-15 0.14852728008899163 1103 16-17 0.18138486950849142 1103 18-19 0.1498411087307261 1103 20-21 0.19862418131532422 1103 22-23 0.21950766811558964 1103 24-25 0.12960381588081304 1103 26-27 0.17945962888950362 1103 28-29 0.16863199772750193 1103 30-31 0.2560620006688552 1103 32-33 0.24963142589627552 1103 34-35 0.23806118156606715 1103 36-37 0.24444107762905531 1103 38-39 0.17328995501772226 1103 40-41 0.42334799603388973 1103 42-43 0.31905001131372046 1103 44-45 0.3522253399268749 1103 46-47 0.33951752704843585 1103 48-49 0.3973788859106264 1103 50-51 0.4852846371052024 1103 52-53 0.4568033818304329 1103 54-55 0.4601571891871572 1103 56-57 0.5216831653483425 1103 58-59 0.555991475099539 1103 60-61 0.43793105864554605 1103 62-63 0.3739138159423234 1103 64-65 0.449961564896725 1103 66-67 0.43133305295904023 1103 68-69 0.4685878127025802 1103 70-71 0.18623179130583623 1103 72-73 0.12852937720948177 1103 74-75 0.17143305768232864 1103 76-77 0.13572483894592935 1103 78-79 0.29723300902124805 1103 80-81 0.1035372847941396 1103 82-83 0.12779854106620903 1103 84-85 0.20964369019079854 1103 86-87 0.36506832626342245 1103 88-89 0.31346840404304643 1103 90-91 0.4872520389225272 1103 92-93 0.47918061665257383 1103 94-95 0.2802801270927979 1103 96-97 0.0892434281596941 1103 98-99 0.21878330208734198 1103 100-101 -0.3428493677602944 1104 1 0.11082488425680737 1104 2 0.09527722676111239 1104 3 0.11915393940482488 1104 4 0.05360100393157552 1104 5 0.1407506042825304 1104 6 0.1537278032115026 1104 7 0.12270737763486039 1104 8 0.11393696279298382 1104 9 0.1425224516232646 1104 10-11 0.14242967107426807 1104 12-13 0.08334560786887124 1104 14-15 0.08561831707624634 1104 16-17 0.14550981138382468 1104 18-19 0.11841063230804139 1104 20-21 0.13148196225301945 1104 22-23 0.1529086652166427 1104 24-25 0.1249906591529708 1104 26-27 0.1472186437424412 1104 28-29 0.20175523712993737 1104 30-31 0.14614555454327416 1104 32-33 0.17548579109936924 1104 34-35 0.1492013744914189 1104 36-37 0.29791280611376436 1104 38-39 0.19907465153412573 1104 40-41 0.26688960358691816 1104 42-43 0.31560743956548976 1104 44-45 0.24292264175547018 1104 46-47 0.17179724399343144 1104 48-49 0.037803096197315256 1104 50-51 0.039326823477431105 1104 52-53 0.06836687338873304 1104 54-55 0.14452729039690126 1104 56-57 0.14933019783450874 1104 58-59 0.08422698277199459 1104 60-61 0.24977125599430394 1104 62-63 0.2656483261900675 1104 64-65 0.10354946180324731 1104 66-67 0.18671572096496192 1104 68-69 -0.004450702240454518 1104 70-71 0.04436269075744548 1104 72-73 0.02897948601454914 1104 74-75 -0.03151846175854445 1104 76-77 0.039611720518333726 1104 78-79 0.13526625902906986 1104 80-81 0.02842672654917422 1104 82-83 0.1503633197285481 1104 84-85 0.00531997003936624 1104 86-87 0.1773909179062052 1104 88-89 0.3791335012986998 1104 90-91 0.3594801260572069 1104 92-93 0.28745605907435845 1104 94-95 0.1692537829969183 1104 96-97 -0.011800577204500229 1104 98-99 -0.19292289834485743 1104 100-101 -0.2854185536481104 1105 1 0.0035708159236023107 1105 2 0.053599632803425834 1105 3 0.013362028791085834 1105 4 0.00942018747267781 1105 5 0.027373717352610072 1105 6 0.06977518306146635 1105 7 0.040914317235866804 1105 8 0.1409343765716855 1105 9 0.10014188702461269 1105 10-11 0.07140232363362031 1105 12-13 0.13525565414401086 1105 14-15 0.17178671341486051 1105 16-17 0.18933397167067767 1105 18-19 0.13950293359950194 1105 20-21 0.08277665067474516 1105 22-23 0.1348377322703911 1105 24-25 0.06890627721956122 1105 26-27 0.16008703708461525 1105 28-29 0.08820956830150095 1105 30-31 0.14063077505623056 1105 32-33 0.1792864995855865 1105 34-35 0.253870414799934 1105 36-37 0.1774713426726109 1105 38-39 0.20551183138881157 1105 40-41 0.20927106976994025 1105 42-43 0.17930949350728298 1105 44-45 0.24804072411733102 1105 46-47 0.2219054473770754 1105 48-49 0.1500983470485835 1105 50-51 0.21524489491419985 1105 52-53 0.2408153034253857 1105 54-55 0.21493135134506502 1105 56-57 0.029978590800133986 1105 58-59 0.0884432155865369 1105 60-61 0.2269329304910528 1105 62-63 0.3169881203477516 1105 64-65 0.16411723721539317 1105 66-67 0.08506141970477898 1105 68-69 0.046259043541923006 1105 70-71 0.20835783917281248 1105 72-73 0.09370104626464837 1105 74-75 0.03450747894530792 1105 76-77 0.1772119256552962 1105 78-79 0.36585375527357655 1105 80-81 0.34696165724252737 1105 82-83 0.1721550118406654 1105 84-85 0.17436014596499305 1105 86-87 0.23262171157358225 1105 88-89 0.4122947027288255 1105 90-91 0.47939243902153805 1105 92-93 0.3335569850810636 1105 94-95 0.08563249510484994 1105 96-97 0.1330197277484615 1105 98-99 0.06066560634829088 1105 100-101 -0.15564396830400185 1106 1 0.05256232715886 1106 2 0.04842273927985019 1106 3 0.07413355092668894 1106 4 0.052501068183950395 1106 5 0.012205006827066711 1106 6 0.09047115555173946 1106 7 0.10035964712619005 1106 8 0.0268341479943075 1106 9 0.11415662467109655 1106 10-11 0.11686578708882678 1106 12-13 0.11306874663148392 1106 14-15 0.07325261475379108 1106 16-17 0.032106069245841695 1106 18-19 0.012041137681507053 1106 20-21 0.10945208987280353 1106 22-23 0.0861399994300669 1106 24-25 0.09443831388887247 1106 26-27 0.13246232886496045 1106 28-29 0.044103234702269845 1106 30-31 0.08184042016574011 1106 32-33 0.056398829409388895 1106 34-35 -0.09022761720709127 1106 36-37 0.11230539075068435 1106 38-39 0.2843482111314799 1106 40-41 0.18777303972230186 1106 42-43 0.16024367528740413 1106 44-45 0.2180468394517021 1106 46-47 0.15512042716192553 1106 48-49 0.0824869059076434 1106 50-51 0.04222695161562484 1106 52-53 0.11001649947341718 1106 54-55 0.06338771925926068 1106 56-57 -0.046161060543305155 1106 58-59 0.02475530801574166 1106 60-61 0.01598882805043189 1106 62-63 0.023947329623673852 1106 64-65 0.06044001858909809 1106 66-67 0.020064851201432532 1106 68-69 -0.17690876669914957 1106 70-71 -0.06227844951015271 1106 72-73 -0.010520932123363025 1106 74-75 -0.008953676564139812 1106 76-77 -0.04131498516755627 1106 78-79 0.05625154591566783 1106 80-81 -0.0023837802591124557 1106 82-83 0.23475700927593834 1106 84-85 0.347981243765485 1106 86-87 0.3254697352016649 1106 88-89 0.3839570114892723 1106 90-91 0.5718373713635643 1106 92-93 0.5686883693792986 1106 94-95 0.2833123547718479 1106 96-97 0.45843543900155126 1106 98-99 0.5909233630518251 1106 100-101 0.12878069784050794 1107 1 0.024030603067089373 1107 2 -0.01579254471343461 1107 3 0.05255680493016257 1107 4 -0.01355510816485861 1107 5 0.04995805204731596 1107 6 0.03684180838051532 1107 7 0.05202016922061148 1107 8 0.035019114907832716 1107 9 0.04857672498422971 1107 10-11 0.13643366705024107 1107 12-13 0.03848892876205667 1107 14-15 0.09381172537344185 1107 16-17 0.12347048659410831 1107 18-19 0.005388264277883081 1107 20-21 0.06192979462093717 1107 22-23 0.08096107956900056 1107 24-25 -0.07821606693907057 1107 26-27 0.03855053798041297 1107 28-29 -0.06790173880623485 1107 30-31 0.11383463344148481 1107 32-33 0.14372989499474187 1107 34-35 0.10802905153393993 1107 36-37 0.13534071852870255 1107 38-39 0.11747819920596925 1107 40-41 0.17404729673319252 1107 42-43 0.1787787960425007 1107 44-45 0.10398304168457884 1107 46-47 0.23937577690668377 1107 48-49 0.2686839322156658 1107 50-51 0.2256550774756434 1107 52-53 0.3756561506831986 1107 54-55 0.26944654847651606 1107 56-57 0.3910226096877878 1107 58-59 0.2631971073051744 1107 60-61 0.29537294108742884 1107 62-63 0.25242921945773134 1107 64-65 0.2908281307632876 1107 66-67 0.48417278079877235 1107 68-69 0.421328315443084 1107 70-71 0.4141801942542358 1107 72-73 0.2551595038396144 1107 74-75 0.40399306524233936 1107 76-77 0.4495974528185407 1107 78-79 0.5667331035213437 1107 80-81 0.40805438931124627 1107 82-83 0.3503273635950279 1107 84-85 0.4796304601775674 1107 86-87 0.3679127791078791 1107 88-89 0.37998229555693896 1107 90-91 0.4177141443846324 1107 92-93 0.4697117071836665 1107 94-95 0.31734778916046125 1107 96-97 0.21340237731864065 1107 98-99 0.34520080350484506 1107 100-101 -0.02429427420520014 1108 1 0.06519269165185904 1108 2 0.04484463551137452 1108 3 0.003071062660914947 1108 4 0.03673298954591786 1108 5 0.009403157884179336 1108 6 0.028666756906496005 1108 7 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-0.33185598205248823 2316 76-77 -0.28226019891862464 2316 78-79 -0.09302386045249023 2316 80-81 0.03302404681102544 2316 82-83 -0.08491927406124589 2316 84-85 -0.049352794709299985 2316 86-87 -0.23096528844488873 2316 88-89 -0.6110644466946233 2316 90-91 -0.8039829634570523 2316 92-93 -0.7118533737790074 2316 94-95 -0.4539146561983678 2316 96-97 -0.68938814595381 2316 98-99 -0.5253990405889759 2316 100-101 -0.1972998098613381 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 14.0 5 40.0 6 74.0 7 222.0 8 430.0 9 897.0 10 1541.0 11 2412.0 12 3473.0 13 4988.0 14 6119.0 15 8265.0 16 10815.0 17 15288.0 18 15144.0 19 11136.0 20 10307.0 21 10470.0 22 10983.0 23 11873.0 24 13786.0 25 15714.0 26 18605.0 27 22454.0 28 27714.0 29 33742.0 30 41231.0 31 50455.0 32 64215.0 33 84154.0 34 118539.0 35 181020.0 36 306099.0 37 509075.0 38 412536.0 39 37811.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.1099023206214 18.567745508933946 14.633703547052704 23.688648623391945 2 16.121916118520772 19.808046673363577 39.20050328693516 24.86953392118049 3 19.75406070971413 23.390910277074592 28.998708314274946 27.856320698936337 4 13.868620573745167 16.07312018012783 34.944197527510774 35.114061718616234 5 14.76484577621678 36.45541647042389 33.210680586928795 15.569057166430527 6 33.99578104302046 34.42737772276243 16.570683536027264 15.006157698189845 7 30.014789146735595 31.762857559999357 19.731796956510983 18.49055633675406 8 27.12176235795944 34.57027318449764 19.056802643711556 19.25116181383137 9 26.740998822785862 16.863895710565387 19.6645691527211 36.73053631392765 10-11 25.937320986570445 25.86849252078557 26.084193850838673 22.109992641805317 12-13 26.634045498574682 23.012717502074796 26.929585908741892 23.423651090608626 14-15 23.290190864558387 24.479571637630425 25.015121610734088 27.215115887077097 16-17 23.14325648333211 27.638158170772552 25.930854643705043 23.2877307021903 18-19 23.269107128262352 26.06667829168497 28.087006228762412 22.577208351290267 20-21 23.58987741354364 25.30857610444465 28.302590113632448 22.79895636837926 22-23 23.342599387771042 25.101217618729105 28.41492748273589 23.141255510763965 24-25 23.273225023094486 25.37376199864234 28.363227421670956 22.989785556592217 26-27 22.83095964059437 25.773758188924667 28.173174959958793 23.222107210522182 28-29 22.93799568985379 26.04240687178746 28.180867044649254 22.838730393709493 30-31 23.096659562104126 25.553942450133054 28.050175932510214 23.299222055252606 32-33 22.790176979861247 25.63945234077415 28.05177122950728 23.518599449857323 34-35 23.442524937088805 25.712664099687714 27.86305672619228 22.9817542370312 36-37 23.59248763922775 25.39127384426385 27.782911336976934 23.233327179531468 38-39 23.038858834805016 25.697951888507202 27.921617181130433 23.34157209555735 40-41 23.347597365237156 25.563607944650187 27.997362908601207 23.09143178151145 42-43 23.260214836771933 25.60919616061798 27.587535906585032 23.54305309602506 44-45 23.167654215651012 25.831332244042265 27.82166929288162 23.179344247425103 46-47 23.637888470960643 26.043879474657 27.168914610783872 23.149317443598484 48-49 23.402135538573884 26.145201780431982 26.97810887429367 23.47455380670046 50-51 23.459313577597708 26.326488640629865 27.005145403236053 23.209052378536374 52-53 23.880317930507452 26.033185627693996 26.90203044121795 23.184466000580603 54-55 23.365178422882288 26.252845419220648 26.907782952173676 23.474193205723385 56-57 23.351111999289888 26.525515764822977 26.789624993997517 23.333747241889615 58-59 23.373760823768126 25.998241130100592 26.947865951412307 23.680132094718974 60-61 23.097771183651354 26.22996506552125 27.212074333818194 23.460189417009207 62-63 23.09992459628453 26.315799685627866 27.31047873962047 23.273796978467136 64-65 22.970581772621728 26.37919919984983 27.046953041138178 23.60326598639027 66-67 22.923317627707547 26.40189505550488 26.990522539678995 23.684264777108577 68-69 23.279090252931724 26.027510884834186 27.19925552390783 23.494143338326257 70-71 23.24995501169696 26.14220572193009 26.809269482117937 23.798569784255015 72-73 23.108369182503115 26.27144705179888 26.851711277439833 23.768472488258176 74-75 23.029330433386512 26.637780376563008 26.617384062354034 23.71550512769645 76-77 23.172213148223765 26.33677011024908 26.790988547317664 23.700028194209494 78-79 23.160034574382944 26.393670676092164 26.854541186103116 23.591753563421776 80-81 23.23061938326034 26.397748720279512 26.66370939224858 23.70792250421157 82-83 23.196059057866094 26.488682063154606 26.730091931710643 23.585166947268654 84-85 22.9808255244472 26.51865232020807 26.6174083148085 23.883113840536232 86-87 23.18686673353043 26.417619790896573 26.57492201048698 23.82059146508602 88-89 23.02523697253734 26.496605215903983 26.399493107913763 24.078664703644918 90-91 22.890507863649667 26.431621528046616 26.598794388095225 24.07907622020849 92-93 22.940542197573105 26.204971074097553 26.676463041442112 24.178023686887233 94-95 22.826992205746183 25.95114488561815 26.9109600237092 24.31090288492647 96-97 22.952595667444523 25.73714122737792 26.866287002576094 24.443976102601464 98-99 22.652150904555203 26.051975605603552 26.566037113514074 24.72983637632717 100-101 22.91583702418134 25.326937749771904 27.151585322494665 24.60563990355209 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 215.0 1 164.5 2 168.5 3 265.0 4 265.5 5 239.5 6 417.5 7 904.5 8 1524.0 9 1830.5 10 1725.0 11 1598.0 12 1639.5 13 1762.0 14 1915.5 15 2041.0 16 2157.0 17 2346.5 18 2538.0 19 2787.5 20 3089.0 21 3622.0 22 4497.5 23 5474.5 24 6729.0 25 8327.5 26 10507.5 27 13213.0 28 15467.5 29 17673.0 30 20157.5 31 23602.0 32 29372.5 33 31574.0 34 31192.5 35 33225.5 36 36367.5 37 39976.0 38 42749.0 39 45111.5 40 48493.5 41 52765.0 42 55584.5 43 58201.0 44 61165.0 45 65177.5 46 68613.5 47 71608.0 48 75634.5 49 77350.0 50 77010.5 51 76917.5 52 78626.5 53 82239.5 54 84965.5 55 81018.5 56 74755.5 57 69198.0 58 64518.5 59 58692.0 60 49069.5 61 41307.0 62 34902.0 63 26131.0 64 18584.5 65 14185.5 66 10739.0 67 8804.5 68 6820.5 69 5078.0 70 4039.0 71 2840.0 72 2101.0 73 1696.0 74 1320.0 75 1035.0 76 733.5 77 485.5 78 341.0 79 212.0 80 123.0 81 86.0 82 57.5 83 35.5 84 23.5 85 12.5 86 7.5 87 5.0 88 3.5 89 3.0 90 3.0 91 1.5 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3428326963830375 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0013581374503006576 16-17 0.0016249144494668584 18-19 7.27573634089638E-5 20-21 0.0 22-23 0.0 24-25 3.6378681704481907E-4 26-27 0.0010186030877254932 28-29 0.0 30-31 0.0029587994452978613 32-33 0.0 34-35 0.010161778422785279 36-37 0.028642148728662085 38-39 0.022093986021855343 40-41 0.013823899047703123 42-43 0.010743837330056988 44-45 0.0029830518997675157 46-47 0.001091360451134457 48-49 6.063113617413651E-4 50-51 4.85049089393092E-5 52-53 7.27573634089638E-5 54-55 0.0 56-57 0.0 58-59 0.0046564712581736835 60-61 0.010501312785360442 62-63 0.0037591304427964634 64-65 0.001649166903936513 66-67 0.0034681009891606076 68-69 0.0030558092631764793 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.21764152641068038 78-79 2.42524544696546E-4 80-81 0.003104314172115789 82-83 3.880392715144736E-4 84-85 0.0 86-87 0.10843272393382573 88-89 0.4050159896432318 90-91 0.3921136838653756 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0012611276324220391 100-101 7.760785430289473E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2061647.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.69906358571206 #Duplication Level Percentage of deduplicated Percentage of total 1 74.32434637150276 39.16823455398311 2 14.074882073059708 14.834662106591445 3 4.802341842970074 7.592367544290168 4 2.2103479701147806 4.659330728945136 5 1.2055786276150824 3.1766432377131353 6 0.7482413702898163 2.365897173021801 7 0.4942947791043835 1.8234210395875183 8 0.3625501221295856 1.5284841551291763 9 0.264926123413531 1.2565222760957286 >10 1.3721383142447177 13.077726212416756 >50 0.09039883465835608 3.214133812183631 >100 0.04488685447946922 4.203596501542858 >500 0.003316396200680574 1.285974538067443 >1k 0.0016581981003402808 1.5156527267343223 >5k 9.212211668557115E-5 0.29735339369787717 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6125 0.2970925672532688 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4819 0.23374515617853106 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3246 0.15744693441699767 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.85049089393092E-5 7 0.0 0.0 0.0 0.0 9.70098178786184E-5 8 0.0 0.0 0.0 0.0 9.70098178786184E-5 9 0.0 0.0 0.0 0.0 9.70098178786184E-5 10-11 0.0 0.0 0.0 0.0 1.455147268179276E-4 12-13 0.0 0.0 0.0 0.0 4.85049089393092E-4 14-15 0.0 0.0 0.0 0.0 5.820589072717104E-4 16-17 0.0 0.0 0.0 0.0 6.790687251503288E-4 18-19 0.0 0.0 0.0 0.0 7.27573634089638E-4 20-21 0.0 0.0 0.0 0.0 9.458457243165293E-4 22-23 0.0 0.0 0.0 2.42524544696546E-5 0.0010186030877254932 24-25 0.0 0.0 0.0 9.70098178786184E-5 0.0010186030877254932 26-27 0.0 0.0 0.0 3.63786817044819E-4 0.0011398653600737663 28-29 0.0 0.0 0.0 0.0019159439031027135 0.0011641178145434209 30-31 0.0 0.0 0.0 0.006790687251503289 0.0011883702690130755 32-33 0.0 0.0 0.0 0.017146485310045805 0.001285380086891694 34-35 0.0 0.0 0.0 0.03257104635274613 0.0015036521771185853 36-37 0.0 0.0 0.0 0.057890608819065534 0.0015036521771185853 38-39 0.0 0.0 0.0 0.09422078561460812 0.0017219242673454766 40-41 0.0 0.0 0.0 0.13634729902839815 0.0017946816307544404 42-43 0.0 0.0 0.0 0.181578126614304 0.001818934085224095 44-45 0.0 0.0 0.0 0.23743152925791855 0.002012953720981332 46-47 0.0 0.0 0.0 0.3051443821371942 0.0022069733567385685 48-49 0.0 0.0 0.0 0.37879908636153525 0.002303983174617187 50-51 0.0 0.0 0.0 0.4607723824689678 0.002376740538026151 52-53 0.0 0.0 0.0 0.5425759113951127 0.0024737503559047695 54-55 0.0 0.0 0.0 0.6362873954658581 0.0025950126282530423 56-57 0.0 0.0 0.0 0.7466845682117258 0.0027890322640102792 58-59 0.0 0.0 0.0 0.8752953342643042 0.002861789627419243 60-61 0.0 0.0 0.0 1.015256249008681 0.0028860420818888977 62-63 0.0 0.0 0.0 1.161086257734714 0.003104314172115789 64-65 0.0 0.0 0.0 1.314361769982931 0.0032013239899944075 66-67 0.0 0.0 0.0 1.5027790887576777 0.003298333807873026 68-69 0.0 0.0 0.0 1.7109136530162536 0.003346838716812335 70-71 0.0 0.0 0.0 1.9410694459332758 0.003346838716812335 72-73 0.0 0.0 0.0 2.178961771826118 0.003589363261508881 74-75 0.0 0.0 0.0 2.4303627148585574 0.0038076353517357726 76-77 0.0 0.0 0.0 2.709411455986403 0.0039531500785537 78-79 4.85049089393092E-5 0.0 0.0 3.0297136221671312 0.004098664805371627 80-81 4.85049089393092E-5 0.0 0.0 3.3817379987941676 0.0041956746232502465 82-83 4.85049089393092E-5 0.0 0.0 3.7434633572090665 0.00426843198665921 84-85 4.85049089393092E-5 0.0 0.0 4.121340850300754 0.004316936895598519 86-87 4.85049089393092E-5 0.0 0.0 4.524295381314066 0.004413946713477137 88-89 4.85049089393092E-5 0.0 0.0 4.960524279859744 0.004462451622416447 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4120 0.0 43.671833 1 GTATCAA 6955 0.0 38.052616 1 TATCAAC 9100 0.0 28.989258 2 TCAACGC 9080 0.0 28.948606 4 ATCAACG 9115 0.0 28.941553 3 CAACGCA 9240 0.0 28.44733 5 AACGCAG 9535 0.0 27.616968 6 ACGCAGA 10975 0.0 23.90696 7 TAAGGTG 1175 0.0 23.420374 5 CGCAGAG 11200 0.0 23.384321 8 GTAAGGT 1110 0.0 22.227165 4 GTACATG 7855 0.0 21.815401 1 TACATGG 7700 0.0 21.44333 2 ACATGGG 7490 0.0 20.71427 3 AGAGTAC 10590 0.0 20.431192 10-11 GAGTACT 7050 0.0 20.291914 12-13 GCAGAGT 12875 0.0 20.158321 9 TGTACGC 120 0.0047959504 19.769354 2 GGTAAGG 1235 0.0 19.593271 3 AGTACTT 7260 0.0 19.508886 12-13 >>END_MODULE