FastQCFastQC Report
Fri 27 May 2016
SRR939076_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939076_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2027271
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58890.290489036739538No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46370.22873113658706704No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29270.14438128893473048No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA21320.10516600888583717No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA20700.10210771031598637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41600.042.124431
GTATCAA65750.038.1363531
ATCAACG84100.029.5668143
TCAACGC84100.029.5668144
TATCAAC86150.028.9175172
CAACGCA86450.028.817175
TAAGGTG10150.027.9972745
AACGCAG89650.027.7755246
GTAAGGT10300.026.6705534
TGTACGC2007.4396667E-1026.049132
TACATGG79600.024.3355272
GTACGCT1951.6201739E-824.2888323
GTACATG81800.024.2674431
ACGCAGA104050.023.795047
CGCAGAG105300.023.6474448
AGGTAAG11050.023.1452582
ACATGGG80550.022.8143143
GGTAAGG11550.022.5539153
TACGCTC1903.3822107E-722.4352114
GAGTACT61000.020.95447712-13
Produced by FastQC (version 0.11.5)