##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939076_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2027271 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.25641219156196 34.0 31.0 34.0 31.0 34.0 2 32.36903304984879 34.0 31.0 34.0 31.0 34.0 3 32.436003375967005 34.0 31.0 34.0 31.0 34.0 4 35.85508005589781 37.0 35.0 37.0 35.0 37.0 5 35.88715716842987 37.0 37.0 37.0 35.0 37.0 6 35.96113099827305 37.0 37.0 37.0 35.0 37.0 7 35.895037713260834 37.0 37.0 37.0 35.0 37.0 8 35.88759223606513 37.0 37.0 37.0 35.0 37.0 9 37.59699122613602 39.0 38.0 39.0 35.0 39.0 10-11 37.511781355329404 39.0 38.0 39.0 35.0 39.0 12-13 37.425668299896756 39.0 38.0 39.0 35.0 39.0 14-15 38.77812857777771 40.5 38.5 41.0 35.0 41.0 16-17 38.69160684486682 40.0 38.0 41.0 35.0 41.0 18-19 38.58093170572657 40.0 38.0 41.0 34.0 41.0 20-21 38.54813465984567 40.0 38.5 41.0 34.0 41.0 22-23 38.58724733891029 40.0 38.5 41.0 34.0 41.0 24-25 38.53902364311431 40.5 38.0 41.0 34.0 41.0 26-27 38.43242467336631 40.0 38.0 41.0 34.0 41.0 28-29 38.29677679994436 40.0 38.0 41.0 34.0 41.0 30-31 38.08628866096343 40.0 38.0 41.0 33.5 41.0 32-33 37.890367395380295 40.0 38.0 41.0 33.0 41.0 34-35 37.58995319323366 40.0 37.5 41.0 32.5 41.0 36-37 37.332669879853256 40.0 37.0 41.0 32.0 41.0 38-39 37.140557182537506 40.0 37.0 41.0 31.0 41.0 40-41 36.935072074725085 40.0 37.0 41.0 31.0 41.0 42-43 36.72218144490796 40.0 36.0 41.0 30.5 41.0 44-45 36.45579821346036 40.0 36.0 41.0 30.0 41.0 46-47 36.334927348144376 40.0 36.0 41.0 30.0 41.0 48-49 36.20373793143591 40.0 36.0 41.0 29.5 41.0 50-51 35.05507280477055 38.5 34.5 40.0 27.5 40.5 52-53 35.16798888752416 38.5 35.0 39.5 27.5 40.5 54-55 35.66087686352737 39.0 35.0 41.0 28.0 41.0 56-57 35.5354294023838 39.0 35.0 41.0 28.0 41.0 58-59 35.3015043376046 39.0 35.0 41.0 27.5 41.0 60-61 34.985602319571484 38.0 35.0 40.5 26.5 41.0 62-63 34.58354704427775 37.5 34.5 40.0 26.0 41.0 64-65 34.18757704322708 37.0 34.0 40.0 26.0 41.0 66-67 33.796568638332026 36.0 34.0 39.0 25.0 41.0 68-69 33.379309919591414 36.0 34.0 39.0 23.5 41.0 70-71 32.940876182809305 35.0 33.0 38.5 23.0 40.5 72-73 32.507576441432846 35.0 33.0 37.5 21.5 40.0 74-75 32.07884417031566 35.0 33.0 37.0 20.0 39.0 76-77 31.667805389609974 35.0 32.0 36.5 19.5 39.0 78-79 31.266569442368585 35.0 32.0 36.0 18.0 38.5 80-81 30.8572687124711 35.0 31.5 35.5 13.5 37.0 82-83 30.496412171830997 35.0 31.0 35.0 8.5 37.0 84-85 30.150132123430957 34.5 31.0 35.0 6.0 36.5 86-87 29.779292457693124 34.0 30.5 35.0 2.0 36.0 88-89 29.388371855563463 34.0 30.0 35.0 2.0 36.0 90-91 29.00289897107984 34.0 29.5 35.0 2.0 35.0 92-93 28.623319230630734 34.0 29.0 35.0 2.0 35.0 94-95 28.16841581613904 34.0 29.0 35.0 2.0 35.0 96-97 27.600493964546427 34.0 28.0 35.0 2.0 35.0 98-99 26.917049077306388 34.0 26.5 35.0 2.0 35.0 100-101 24.029331303017702 30.0 13.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19865164820453174 1101 2 0.3004813679283913 1101 3 0.24393322160326392 1101 4 0.3047798258109822 1101 5 0.19139499858393805 1101 6 0.1116064212587915 1101 7 0.10271100634325592 1101 8 0.22163506019908397 1101 9 0.19425294868290166 1101 10-11 0.24966760029274582 1101 12-13 0.2475348910755244 1101 14-15 0.25278801001437756 1101 16-17 0.3162871977392996 1101 18-19 0.38134137149146596 1101 20-21 0.4018231574760449 1101 22-23 0.3764357284053901 1101 24-25 0.36900067773044753 1101 26-27 0.3373253923107882 1101 28-29 0.3868930667505168 1101 30-31 0.3875177949838431 1101 32-33 0.36121569765197137 1101 34-35 0.3821244352583122 1101 36-37 0.4278498817840557 1101 38-39 0.35758677395171645 1101 40-41 0.3007841171758514 1101 42-43 0.33196921338537067 1101 44-45 0.24224854217751357 1101 46-47 0.30832989626436813 1101 48-49 0.12686352103818166 1101 50-51 0.07742644993346914 1101 52-53 0.08575245236672657 1101 54-55 0.2612760066843407 1101 56-57 0.2143495950611154 1101 58-59 0.11713412734614082 1101 60-61 0.07959815417540028 1101 62-63 -0.07603308018755683 1101 64-65 -0.08734617325480798 1101 66-67 -0.09412854805507465 1101 68-69 -0.2505128774919072 1101 70-71 -0.20511389803122881 1101 72-73 -0.21916965451369919 1101 74-75 -0.362938852675768 1101 76-77 -0.49892257154861497 1101 78-79 -0.5302430808884786 1101 80-81 -0.6319740223235328 1101 82-83 -0.7534715332002762 1101 84-85 -0.881742330034843 1101 86-87 -0.8553263636467108 1101 88-89 -0.8794809350344828 1101 90-91 -0.9081616679472937 1101 92-93 -0.9928930349706313 1101 94-95 -1.024335076955765 1101 96-97 -1.4137983507945897 1101 98-99 -1.6506869114044278 1101 100-101 -1.5937335489625042 1102 1 0.16426737302747796 1102 2 0.20898771545549977 1102 3 0.20091017508411824 1102 4 0.06134499816397465 1102 5 0.2875611362900159 1102 6 0.11255965232641074 1102 7 0.13013614372607663 1102 8 0.17258957693480426 1102 9 0.25895970615447084 1102 10-11 0.2896096350278228 1102 12-13 0.28571280550145417 1102 14-15 0.36399141372886135 1102 16-17 0.26054200613801726 1102 18-19 0.27355554910467816 1102 20-21 0.2847410363440588 1102 22-23 0.3977364939289103 1102 24-25 0.4190333704337377 1102 26-27 0.3742332503142549 1102 28-29 0.4009647041774187 1102 30-31 0.4646032404862055 1102 32-33 0.4375857606864386 1102 34-35 0.4444021983427362 1102 36-37 0.43139557075522816 1102 38-39 0.2340271813983037 1102 40-41 0.24366004653022344 1102 42-43 0.3511169439975035 1102 44-45 0.47673787480555774 1102 46-47 0.6872929682030104 1102 48-49 0.6117468191074735 1102 50-51 0.552808660694879 1102 52-53 0.5083056422929602 1102 54-55 0.47741977376536227 1102 56-57 0.5095973065718908 1102 58-59 0.4213925356155528 1102 60-61 0.4367659375366557 1102 62-63 0.43094166929082434 1102 64-65 0.3786561703344873 1102 66-67 0.35073924853018923 1102 68-69 0.35369419588668194 1102 70-71 0.39879333917821214 1102 72-73 0.26721512023724614 1102 74-75 0.04544535638121516 1102 76-77 0.1684429475674314 1102 78-79 0.005380945677099902 1102 80-81 0.07735157128779946 1102 82-83 -0.10743662547451294 1102 84-85 -0.15481823587985488 1102 86-87 -0.09834610416215384 1102 88-89 -0.16425642064968926 1102 90-91 -0.1739133980892369 1102 92-93 -0.44904624252889036 1102 94-95 -0.4035001824540174 1102 96-97 -0.445650778135974 1102 98-99 -0.6002777016127823 1102 100-101 -0.7343121730772282 1103 1 0.1528843826960511 1103 2 0.19435854179121037 1103 3 0.29733646762726096 1103 4 0.22387684325805424 1103 5 0.1896021874034659 1103 6 0.15718133023671044 1103 7 0.1012419097636652 1103 8 0.1157199911938207 1103 9 0.10208940879126516 1103 10-11 0.2167021601480883 1103 12-13 0.18746831820153886 1103 14-15 0.2842617056362897 1103 16-17 0.299482121942674 1103 18-19 0.3206717593842896 1103 20-21 0.3335577272058856 1103 22-23 0.32491648927965144 1103 24-25 0.3782449641828407 1103 26-27 0.23111001157923283 1103 28-29 0.18414082830330614 1103 30-31 0.245443019930768 1103 32-33 0.30386023456041755 1103 34-35 0.2833075336116835 1103 36-37 0.31339170664660543 1103 38-39 0.3103111554288347 1103 40-41 0.2533748757883316 1103 42-43 0.14712198584088299 1103 44-45 0.23247414286890233 1103 46-47 0.27890795330638696 1103 48-49 0.24261444077115613 1103 50-51 0.15084262642315593 1103 52-53 0.18254100422402075 1103 54-55 0.22061162474219032 1103 56-57 0.1618780854186781 1103 58-59 0.14347277932244396 1103 60-61 0.17528450613228586 1103 62-63 0.0628933287938338 1103 64-65 0.07771143254354484 1103 66-67 0.042340861066129776 1103 68-69 0.09695216842755627 1103 70-71 0.112478812833956 1103 72-73 0.07961786520133529 1103 74-75 -0.08941209382895465 1103 76-77 -0.057235287704358484 1103 78-79 -0.14651007175290331 1103 80-81 -0.10703878789165344 1103 82-83 -0.17135794259235126 1103 84-85 -0.17641834214877505 1103 86-87 -0.22928844615830357 1103 88-89 -0.18620640784049058 1103 90-91 -0.1967272649969054 1103 92-93 -0.27626258991438846 1103 94-95 -0.43027333745482466 1103 96-97 -0.4095902119693413 1103 98-99 -0.5697194577066398 1103 100-101 -0.359398753779935 1104 1 0.01337819268111673 1104 2 0.08450599265976422 1104 3 0.16228794628059262 1104 4 0.21437603838511166 1104 5 0.21542188542974827 1104 6 0.14086040823547563 1104 7 0.23496211540768996 1104 8 0.19744749141554507 1104 9 0.18980143064253951 1104 10-11 0.2462259547660821 1104 12-13 0.2252418431276766 1104 14-15 0.3015980660106905 1104 16-17 0.10879520041793 1104 18-19 0.13818471647648067 1104 20-21 0.23140784403854298 1104 22-23 0.23290142595350716 1104 24-25 0.1918834014598474 1104 26-27 0.20568738807022413 1104 28-29 0.2804264715379432 1104 30-31 0.3428230816888913 1104 32-33 0.31483427284303644 1104 34-35 0.29374218818873743 1104 36-37 0.07667008138872689 1104 38-39 -0.010108705948681518 1104 40-41 0.05941426326175758 1104 42-43 0.20730471547603457 1104 44-45 0.22384327899126788 1104 46-47 0.3649271914218275 1104 48-49 0.3644245846628422 1104 50-51 0.32230751836961247 1104 52-53 0.36987626902055837 1104 54-55 0.39642926493702646 1104 56-57 0.3498506982907372 1104 58-59 0.3265116688586289 1104 60-61 0.27780291502860877 1104 62-63 0.30604567730120635 1104 64-65 0.41994735045520315 1104 66-67 0.22268775927651063 1104 68-69 0.14480732839641774 1104 70-71 0.22018426121803714 1104 72-73 0.2936261034831418 1104 74-75 0.11298533776554365 1104 76-77 0.08238248894499023 1104 78-79 0.05155345089512764 1104 80-81 0.042134282688486735 1104 82-83 0.1079331141881461 1104 84-85 0.03315773298120561 1104 86-87 -0.10053732377066282 1104 88-89 -0.2485134861430076 1104 90-91 0.13900880391900117 1104 92-93 -0.04288262206293325 1104 94-95 -0.20946266200498798 1104 96-97 -0.3311943698322537 1104 98-99 -0.42545257609803144 1104 100-101 -0.7456085007130397 1105 1 0.06313392533830608 1105 2 0.19312955853696678 1105 3 0.2697476851948437 1105 4 0.19215256656829638 1105 5 0.24202973027099262 1105 6 0.2523126018703863 1105 7 0.19712495916689932 1105 8 0.25132688622552024 1105 9 0.2220895348996237 1105 10-11 0.23002780087168873 1105 12-13 0.22026064668629175 1105 14-15 0.29873493240233273 1105 16-17 0.21828482054685594 1105 18-19 0.25426410208489614 1105 20-21 0.26816466895500213 1105 22-23 0.33222332208834615 1105 24-25 0.42709231947565485 1105 26-27 0.5269837174997107 1105 28-29 0.38406671098298517 1105 30-31 0.36888905687535356 1105 32-33 0.37514197983127673 1105 34-35 0.28902468460609754 1105 36-37 0.4025791188544119 1105 38-39 0.2977069118253297 1105 40-41 0.21400771618129966 1105 42-43 0.24882206978892185 1105 44-45 0.26046761848112254 1105 46-47 0.3869210502324094 1105 48-49 0.48402825301816677 1105 50-51 0.3698872524445207 1105 52-53 0.39902434086769745 1105 54-55 0.3580809109086971 1105 56-57 0.415728331172339 1105 58-59 0.5017732743264034 1105 60-61 0.37386848125684224 1105 62-63 0.3017239260057494 1105 64-65 0.4327494470477333 1105 66-67 0.4228557729493474 1105 68-69 0.4175922657832061 1105 70-71 0.37008278845721065 1105 72-73 0.5426122278711958 1105 74-75 0.46054573152415657 1105 76-77 0.3955848534051576 1105 78-79 0.5339908249061729 1105 80-81 0.5138522650930568 1105 82-83 0.36305616801556795 1105 84-85 0.13447415248374028 1105 86-87 0.18046511805076904 1105 88-89 0.3307962946042622 1105 90-91 0.4294884267231929 1105 92-93 0.30087531215272634 1105 94-95 0.4338665476661916 1105 96-97 0.5407633643346728 1105 98-99 0.733365926535491 1105 100-101 0.4905853250400831 1106 1 0.18814904296996815 1106 2 0.18688954991716855 1106 3 0.2499224230276269 1106 4 0.24045752273266174 1106 5 0.2225587704168248 1106 6 0.17460467671310909 1106 7 0.23390698160916656 1106 8 0.29398389519934653 1106 9 0.2663553347487735 1106 10-11 0.28439561449932427 1106 12-13 0.30018330488268674 1106 14-15 0.279505854654829 1106 16-17 0.32075006648598503 1106 18-19 0.34818747383351223 1106 20-21 0.3389786792064058 1106 22-23 0.3698942612725844 1106 24-25 0.38783114287313225 1106 26-27 0.35845469604476676 1106 28-29 0.36513764950755245 1106 30-31 0.39911291326775 1106 32-33 0.24955912641404154 1106 34-35 0.2889279317634319 1106 36-37 0.3206658808666063 1106 38-39 0.3878260002816063 1106 40-41 0.5973762873397135 1106 42-43 0.5133282619708481 1106 44-45 0.3716976549613449 1106 46-47 0.3336870589797485 1106 48-49 0.3539007646559966 1106 50-51 0.37121196927753886 1106 52-53 0.3261015584318585 1106 54-55 0.3253828479445957 1106 56-57 0.35730445696862034 1106 58-59 0.4550573186831812 1106 60-61 0.4666760343554657 1106 62-63 0.4115801820665439 1106 64-65 0.2942377201634159 1106 66-67 0.4390841382441053 1106 68-69 0.4325642184534715 1106 70-71 0.421829891918982 1106 72-73 0.448113424088163 1106 74-75 0.5363572185054011 1106 76-77 0.511751621222519 1106 78-79 0.3769777280895603 1106 80-81 0.3055252536784252 1106 82-83 0.3251917231459984 1106 84-85 0.32737856837143653 1106 86-87 0.2604362901015129 1106 88-89 0.32287231924984994 1106 90-91 0.27700951994235723 1106 92-93 0.35758457205944794 1106 94-95 0.34387802057429795 1106 96-97 0.22529948392998023 1106 98-99 0.24030794299666525 1106 100-101 0.12000008578569776 1107 1 0.03749229001308407 1107 2 0.1531718445507977 1107 3 0.07566220633552945 1107 4 0.20832713084224252 1107 5 0.21763847313393114 1107 6 0.20102031209858495 1107 7 0.2680644482418799 1107 8 0.1984283729320211 1107 9 0.049859846849166445 1107 10-11 0.10203498993133309 1107 12-13 0.2437795088120751 1107 14-15 0.2727933429653078 1107 16-17 0.26456534856448144 1107 18-19 0.2171049121331876 1107 20-21 0.1983591294106688 1107 22-23 0.2915151078825744 1107 24-25 0.2601258353505358 1107 26-27 0.3965538306459564 1107 28-29 0.3748307207531596 1107 30-31 0.3878908423880745 1107 32-33 0.32150061890600057 1107 34-35 0.4025896344420161 1107 36-37 0.5150108024264597 1107 38-39 0.47277174700907665 1107 40-41 0.6694096642568468 1107 42-43 0.5284461254800803 1107 44-45 0.6580887022351476 1107 46-47 0.6843647032717328 1107 48-49 0.6151926516491173 1107 50-51 0.5262579088151824 1107 52-53 0.5339072139115402 1107 54-55 0.4731618930527546 1107 56-57 0.41202584520185326 1107 58-59 0.5453643276859808 1107 60-61 0.5313486262077873 1107 62-63 0.5952024453283968 1107 64-65 0.5774414344876462 1107 66-67 0.5504547489237765 1107 68-69 0.5185247062781855 1107 70-71 0.493574374573555 1107 72-73 0.6188449846093746 1107 74-75 0.6314605410034133 1107 76-77 0.6294106307645997 1107 78-79 0.6589168612783745 1107 80-81 0.4896188425797483 1107 82-83 0.5133078971587821 1107 84-85 0.5808612154560642 1107 86-87 0.5785404375531442 1107 88-89 0.6882482616336318 1107 90-91 0.6842913138259341 1107 92-93 0.7583772123176118 1107 94-95 0.6274867218554903 1107 96-97 0.6051019498030108 1107 98-99 0.5556841796246097 1107 100-101 0.5164297539424858 1108 1 0.012881408115198667 1108 2 0.07551403124497114 1108 3 0.10109877157534441 1108 4 0.16670026132187132 1108 5 0.07901727664089009 1108 6 0.18427895057202903 1108 7 0.0586185998664277 1108 8 0.1205150155312964 1108 9 0.19583251317201444 1108 10-11 0.20012279292256352 1108 12-13 0.22030052479970408 1108 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-0.8431276647475414 2316 86-87 -0.9632939197045829 2316 88-89 -0.9017274534478723 2316 90-91 -1.0868755467423021 2316 92-93 -0.98617514918665 2316 94-95 -0.7655863994592451 2316 96-97 -0.9425224508222492 2316 98-99 -1.3634347264636801 2316 100-101 -1.1387343252446378 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6360.0 3 1726.0 4 1603.0 5 1746.0 6 2024.0 7 2404.0 8 3012.0 9 3649.0 10 4387.0 11 5379.0 12 7204.0 13 11655.0 14 9720.0 15 9602.0 16 11665.0 17 16319.0 18 13953.0 19 10515.0 20 10464.0 21 10746.0 22 11495.0 23 12678.0 24 14053.0 25 16199.0 26 18164.0 27 21314.0 28 25280.0 29 30495.0 30 36592.0 31 45636.0 32 58220.0 33 77686.0 34 109882.0 35 171143.0 36 288383.0 37 461252.0 38 412270.0 39 72365.0 40 31.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.88062184951353 18.089753487664975 14.230901224217327 23.79872343860417 2 15.867467553087202 19.42750415694819 39.919888311802474 24.78513997816213 3 19.78827502319878 23.27863573891138 28.75359710720231 28.179492130687528 4 13.607023030613147 15.716125182932581 35.222875228495944 35.45397655795833 5 14.780885978915425 36.35251861101013 33.18652302075266 15.680072389321783 6 33.86616197516823 34.18677731968533 16.872314630524755 15.074746074621679 7 30.063610939008043 31.649910273277726 19.893611506742463 18.392867280971764 8 27.018233087717668 34.455174565499206 19.243672920107933 19.282919426675186 9 26.852676455720196 16.95518111592051 19.750857002938297 36.441285425421 10-11 25.952769883383464 25.759700725527335 26.202189960107596 22.085339430981602 12-13 26.6896769452658 22.936646694969163 26.956237767612972 23.417438592152067 14-15 23.384932304118326 24.35696687302635 25.112365734979274 27.145735087876048 16-17 23.192314233196765 27.55548216595741 25.955926255301513 23.296277345544308 18-19 23.29479604695567 26.06795020062675 28.07076181389786 22.56649193851972 20-21 23.73520436009004 25.276658811204538 28.215878664336113 22.772258164369312 22-23 23.42833951006793 25.124472805086747 28.298455273232864 23.148732411612457 24-25 23.362580889199112 25.334019400321818 28.270017399402835 23.033382311076238 26-27 22.814355895991678 25.727395799460705 28.12407795463765 23.334170349909968 28-29 22.952129005657515 26.005162497700823 28.15684512383452 22.88586337280714 30-31 23.10349151991737 25.544548638214902 27.912089159980308 23.439870681887417 32-33 22.82087336386837 25.573519341296404 28.036449490059656 23.56915780477557 34-35 23.521489576375608 25.654571538663518 27.77983321508038 23.04410566988049 36-37 23.59860948220073 25.34117028636038 27.71271227836143 23.34750795307746 38-39 23.117162124440167 25.68812661480131 27.80847136357033 23.386239897188194 40-41 23.4404119909592 25.530007137475714 27.900739521789124 23.128841349775964 42-43 23.28986739583669 25.529586172873785 27.57593937245433 23.604607058835196 44-45 23.20355967052172 25.795785052190368 27.77728605122171 23.2233692260662 46-47 23.71894247287049 26.051122058805664 27.087932807126673 23.14200266119717 48-49 23.454462552572483 26.16461550400782 26.90590520130089 23.475016742118807 50-51 23.438474314844758 26.312063894351077 26.99046905461382 23.258992736190347 52-53 23.845366752145928 25.979976381839652 26.939107741307012 23.235549124707408 54-55 23.38095456500639 26.261557703917006 26.941898791763613 23.41558893931299 56-57 23.36628971427311 26.514854660550764 26.7938574282721 23.32499819690403 58-59 23.408715064194624 25.97290659011274 26.916266578375875 23.70211176731676 60-61 23.113214090035854 26.195372279450595 27.2356879805887 23.45572564992485 62-63 23.147280322904255 26.21542104296408 27.373363869397593 23.263934764734067 64-65 22.998124758714244 26.324117963413084 27.1215480175082 23.556209260364472 66-67 22.998893040537926 26.33269091331099 26.98937775190023 23.679038294250855 68-69 23.3344403127684 25.983552054777874 27.203321037511724 23.478686594942005 70-71 23.284277011563805 26.152958308987866 26.83855806058971 23.724206618858616 72-73 23.189033625572932 26.184472981806028 26.898722563482515 23.727770829138525 74-75 23.113604780226808 26.566999255073387 26.69264982596888 23.62674613873093 76-77 23.31180750514924 26.27486486030855 26.779897084592342 23.63343054994987 78-79 23.317858384764342 26.366789245179252 26.792496975606323 23.522855394450083 80-81 23.401684326581684 26.38482519708148 26.65800278209347 23.555487694243364 82-83 23.404464722209276 26.42137997439607 26.704441220539866 23.46971408285479 84-85 23.20954798670437 26.59059365554756 26.608020064911514 23.591838292836556 86-87 23.408873496441384 26.37205185010559 26.585090330645933 23.63398432280709 88-89 23.27502235594331 26.42580343182014 26.416134656782038 23.883039555454516 90-91 23.11979787996135 26.41994459998646 26.596742602193835 23.863514917858357 92-93 23.336176839641123 26.09924663614857 26.727454880002384 23.837121644207926 94-95 23.22742702978816 25.883614650826598 26.924105028503696 23.964853290881543 96-97 23.387632908652183 25.653406322383905 26.880211547898693 24.078749221065223 98-99 23.12143042768411 25.95313408201362 26.623281298664715 24.302154191637555 100-101 23.540325721528994 25.284311564767854 27.05343567467116 24.12192703903199 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1658.0 1 926.5 2 254.0 3 315.0 4 274.5 5 301.0 6 523.0 7 1002.5 8 1641.5 9 2019.0 10 1977.0 11 1859.5 12 1993.0 13 2204.5 14 2406.5 15 2556.5 16 2685.5 17 2928.0 18 3122.0 19 3260.0 20 3502.0 21 3905.5 22 4596.0 23 5663.5 24 6822.5 25 8244.0 26 10355.0 27 13016.0 28 15275.5 29 17424.5 30 19632.5 31 22867.0 32 28472.5 33 30755.5 34 30754.0 35 32610.5 36 35481.0 37 38955.0 38 41802.0 39 44492.5 40 47551.0 41 51246.5 42 54225.5 43 56985.5 44 59929.0 45 63898.0 46 66803.0 47 69616.0 48 73809.5 49 75266.5 50 74932.5 51 74754.5 52 76431.5 53 80001.0 54 82499.0 55 78819.0 56 73130.0 57 68128.5 58 63296.0 59 57363.5 60 48243.0 61 40751.0 62 34609.5 63 26212.0 64 18626.0 65 14312.0 66 10941.5 67 8852.0 68 6973.5 69 5344.5 70 4175.5 71 2897.5 72 2130.0 73 1735.0 74 1356.5 75 1057.0 76 793.0 77 518.5 78 376.5 79 268.5 80 176.0 81 125.5 82 95.5 83 77.5 84 55.0 85 35.5 86 27.5 87 23.5 88 20.5 89 14.5 90 9.5 91 10.0 92 11.0 93 8.5 94 5.5 95 5.0 96 2.0 97 1.0 98 1.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.391659526526054 2 0.11582072648402704 3 0.11739920316524036 4 0.1291391234817644 5 0.13135885631472063 6 0.07295521910982794 7 0.08267271617854742 8 0.07956509021240869 9 0.07926912583468121 10-11 0.07754266696460414 12-13 0.08161217715835721 14-15 0.0749776423576325 16-17 0.07658078273698977 18-19 0.08600231542798176 20-21 0.08467047572820803 22-23 0.0951525474393902 24-25 0.10859426292784734 26-27 0.10958081085360566 28-29 0.10302026714731283 30-31 0.09263685022870646 32-33 0.09066375437718982 34-35 0.33959445974415853 36-37 0.5305654744728259 38-39 0.5121170282611451 40-41 0.4809914412034701 42-43 0.5113277899205385 44-45 0.5212672602725535 46-47 0.5184062712878545 48-49 0.5253614341644506 50-51 0.5243255588424044 52-53 0.5229937191426307 54-55 0.5173703959658082 56-57 0.48871117872252895 58-59 0.47825377070949077 60-61 0.42682996007933816 62-63 0.5032627606274642 64-65 0.5293076258674839 66-67 0.5394937332009386 68-69 0.5561417294481102 70-71 0.5501484507991284 72-73 0.5371260181791186 74-75 0.5408749002970002 76-77 0.5164085117381939 78-79 0.4895497444594235 80-81 0.49746679156363405 82-83 0.5126596296203123 84-85 0.5040026715717829 86-87 0.5063210591973151 88-89 0.5164331754363378 90-91 0.5349802764405943 92-93 0.5416641386376069 94-95 0.5359914880644965 96-97 0.5374466462549901 98-99 0.5388031496529078 100-101 0.5142627699996696 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2027271.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.283502862422786 #Duplication Level Percentage of deduplicated Percentage of total 1 75.4955549308767 40.98163172190442 2 13.522124468723916 14.680565646080238 3 4.636765393952764 7.550996026050534 4 2.105295969818693 4.571313592156007 5 1.1222729304995354 3.0460452917595564 6 0.6840889803506834 2.228084767381093 7 0.4790857160959675 1.8204515588728925 8 0.31889082563957677 1.3848408837125066 9 0.23500115521295448 1.1481017293507567 >10 1.272956011531351 12.53739223875371 >50 0.0836408733520052 3.103348151833073 >100 0.03950088277685862 3.9240445989241803 >500 0.003275226454352085 1.311453820615557 >1k 0.0014556562019342578 1.420893913497652 >5k 9.097851262089111E-5 0.2908360591077013 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5889 0.290489036739538 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4637 0.22873113658706704 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2927 0.14438128893473048 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2132 0.10516600888583717 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2070 0.10210771031598637 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.932739628791612E-5 2 0.0 0.0 0.0 0.0 4.932739628791612E-5 3 0.0 0.0 0.0 0.0 4.932739628791612E-5 4 0.0 0.0 0.0 0.0 4.932739628791612E-5 5 0.0 0.0 0.0 0.0 4.932739628791612E-5 6 0.0 0.0 0.0 0.0 9.865479257583224E-5 7 0.0 0.0 0.0 0.0 9.865479257583224E-5 8 0.0 0.0 0.0 0.0 9.865479257583224E-5 9 0.0 0.0 0.0 0.0 9.865479257583224E-5 10-11 0.0 0.0 0.0 0.0 1.4798218886374835E-4 12-13 0.0 0.0 0.0 0.0 6.412561517429096E-4 14-15 0.0 0.0 0.0 0.0 9.372205294704062E-4 16-17 0.0 0.0 0.0 0.0 0.0012825123034858191 18-19 0.0 0.0 0.0 0.0 0.0012825123034858191 20-21 0.0 0.0 0.0 0.0 0.0013811670960616515 22-23 0.0 0.0 0.0 7.399109443187419E-5 0.0013811670960616515 24-25 0.0 0.0 0.0 3.4529177401541286E-4 0.0014304944923495674 26-27 0.0 0.0 0.0 6.905835480308257E-4 0.0015044855867814416 28-29 0.0 0.0 0.0 0.002589688305115596 0.0015291492849253997 30-31 0.0 0.0 0.0 0.007325118348755544 0.0015538129830693578 32-33 0.0 0.0 0.0 0.015982076397284824 0.001603140379357274 34-35 0.0 0.0 0.0 0.031742179511274025 0.001627804077501232 36-37 0.0 0.0 0.0 0.05724444339212666 0.001627804077501232 38-39 0.0 0.0 0.0 0.09660770562988372 0.0018251136626528965 40-41 0.0 0.0 0.0 0.13804271851173328 0.0018251136626528965 42-43 0.0 0.0 0.0 0.1837198874743436 0.0018744410589408126 44-45 0.0 0.0 0.0 0.24182756030150876 0.0018991047570847707 46-47 0.0 0.0 0.0 0.30977604868811326 0.0019484321533726867 48-49 0.0 0.0 0.0 0.3830518958738126 0.002022423247804561 50-51 0.0 0.0 0.0 0.46799367228160416 0.0022690602292441413 52-53 0.0 0.0 0.0 0.5545139253706091 0.0022937239273880996 54-55 0.0 0.0 0.0 0.6500857556784465 0.002466369814395806 56-57 0.0 0.0 0.0 0.7592719473617489 0.0025896883051155966 58-59 0.0 0.0 0.0 0.8862653291049889 0.002688343097691429 60-61 0.0 0.0 0.0 1.0281802482253235 0.0027130067958353868 62-63 0.0 0.0 0.0 1.1768530206371028 0.0028116615884112186 64-65 0.0 0.0 0.0 1.3299899224129383 0.002860988984699135 66-67 0.0 0.0 0.0 1.5180506207606186 0.002910316380987051 68-69 0.0 0.0 0.0 1.7301090974023698 0.002910316380987051 70-71 0.0 0.0 0.0 1.962465797616599 0.002984307475418925 72-73 0.0 0.0 0.0 2.200618466894658 0.003329599249434338 74-75 0.0 0.0 0.0 2.453100744794357 0.003699554721593709 76-77 0.0 0.0 0.0 2.7339462755596067 0.003798209514169541 78-79 0.0 2.466369814395806E-5 0.0 3.0517626898426506 0.0039461917030332895 80-81 0.0 4.932739628791612E-5 0.0 3.405514112321441 0.004044846495609122 82-83 0.0 4.932739628791612E-5 0.0 3.77421173587547 0.00431614717519266 84-85 0.0 4.932739628791612E-5 0.0 4.150234477778255 0.004365474571480577 86-87 0.0 4.932739628791612E-5 0.0 4.549909706201095 0.004488793062200367 88-89 0.0 4.932739628791612E-5 0.0 4.9911925933927925 0.004612111552920157 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4160 0.0 42.12443 1 GTATCAA 6575 0.0 38.136353 1 ATCAACG 8410 0.0 29.566814 3 TCAACGC 8410 0.0 29.566814 4 TATCAAC 8615 0.0 28.917517 2 CAACGCA 8645 0.0 28.81717 5 TAAGGTG 1015 0.0 27.997274 5 AACGCAG 8965 0.0 27.775524 6 GTAAGGT 1030 0.0 26.670553 4 TGTACGC 200 7.4396667E-10 26.04913 2 TACATGG 7960 0.0 24.335527 2 GTACGCT 195 1.6201739E-8 24.288832 3 GTACATG 8180 0.0 24.267443 1 ACGCAGA 10405 0.0 23.79504 7 CGCAGAG 10530 0.0 23.647444 8 AGGTAAG 1105 0.0 23.145258 2 ACATGGG 8055 0.0 22.814314 3 GGTAAGG 1155 0.0 22.553915 3 TACGCTC 190 3.3822107E-7 22.435211 4 GAGTACT 6100 0.0 20.954477 12-13 >>END_MODULE