FastQCFastQC Report
Fri 27 May 2016
SRR939076_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939076_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2027271
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59300.2925114599873426No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46070.22725131469842957No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30410.15000461211155292No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA21150.10432744314894259No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA20350.10038125144590931No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43300.043.0395471
GTATCAA67550.035.632381
ATCAACG87250.027.4638653
TATCAAC87500.027.331172
TCAACGC87800.027.2918244
CAACGCA88900.026.954135
AACGCAG92250.025.9238726
ACGCAGA105700.022.4904637
CGCAGAG107300.022.15518
TACATGG74550.022.1496732
GTACATG78550.022.086861
ACATGGG75050.021.7492083
GTGGTAT24150.021.1176431
GAGTACT65150.020.7211712-13
TGGTATC25200.019.9591052
GTACTTT68250.019.64093814-15
AGTACTT68250.019.64093812-13
TAAGGTG10650.019.1581955
AGAGTAC100700.019.08411410-11
CATGGGG48550.019.0581654
Produced by FastQC (version 0.11.5)