##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939076_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2027271 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.54390409570304 34.0 31.0 34.0 31.0 34.0 2 32.76382091984742 34.0 31.0 34.0 31.0 34.0 3 32.86960549428271 34.0 31.0 34.0 31.0 34.0 4 36.30451873479175 37.0 37.0 37.0 35.0 37.0 5 36.26188753254991 37.0 37.0 37.0 35.0 37.0 6 36.30022379839696 37.0 37.0 37.0 35.0 37.0 7 36.254488916380694 37.0 37.0 37.0 35.0 37.0 8 36.224847097403355 37.0 37.0 37.0 35.0 37.0 9 37.98383689205834 39.0 38.0 39.0 35.0 39.0 10-11 37.903153549772085 39.0 38.0 39.0 35.0 39.0 12-13 37.82013036244291 39.0 38.0 39.0 35.0 39.0 14-15 39.260794684085155 40.5 39.0 41.0 36.0 41.0 16-17 39.342890269727135 41.0 39.0 41.0 36.0 41.0 18-19 39.362698672254474 41.0 39.0 41.0 36.0 41.0 20-21 39.31942942014166 41.0 39.0 41.0 36.0 41.0 22-23 39.24808350733572 41.0 39.0 41.0 36.0 41.0 24-25 39.17356214339375 41.0 39.0 41.0 36.0 41.0 26-27 39.02565147925462 40.5 39.0 41.0 35.0 41.0 28-29 38.87475650764007 40.0 38.0 41.0 35.0 41.0 30-31 38.67416147125865 40.0 38.0 41.0 35.0 41.0 32-33 38.58914521048247 40.0 38.0 41.0 35.0 41.0 34-35 38.4906105301166 40.0 38.0 41.0 34.0 41.0 36-37 38.34295266888344 40.0 38.0 41.0 34.0 41.0 38-39 38.19752218622966 40.0 38.0 41.0 34.0 41.0 40-41 37.995653023202124 40.0 38.0 41.0 33.0 41.0 42-43 37.796794804444005 40.0 37.0 41.0 33.0 41.0 44-45 37.54014288173609 40.0 37.0 41.0 33.0 41.0 46-47 37.2348193704739 40.0 37.0 41.0 32.0 41.0 48-49 36.876990298780974 40.0 36.0 41.0 31.0 41.0 50-51 36.59108254397167 39.0 36.0 41.0 31.0 41.0 52-53 36.288808945621966 39.0 35.0 41.0 30.0 41.0 54-55 36.006454736441256 39.0 35.0 40.0 30.0 41.0 56-57 35.71933007476554 38.5 35.0 40.0 29.0 41.0 58-59 35.34372340945044 38.0 35.0 40.0 28.0 41.0 60-61 34.92921913251854 37.5 34.0 40.0 28.0 41.0 62-63 34.492604343474554 37.0 34.0 40.0 26.5 41.0 64-65 34.011109516191965 36.0 33.5 39.0 26.0 41.0 66-67 33.570540643061534 36.0 33.0 39.0 26.0 40.0 68-69 33.05753498175626 35.0 33.0 38.5 24.5 40.0 70-71 32.53643296826127 35.0 32.0 37.5 23.5 40.0 72-73 31.96462017164947 35.0 31.5 37.0 22.0 39.0 74-75 31.431578708519975 35.0 31.0 36.0 20.0 39.0 76-77 29.491153871386707 32.5 28.5 34.5 18.0 36.5 78-79 30.478629398832226 34.0 30.5 35.0 18.0 37.0 80-81 30.523311387574726 34.0 31.0 35.0 18.0 37.0 82-83 30.15925053927176 34.0 30.0 35.0 14.5 36.5 84-85 29.70439275262163 34.0 30.0 35.0 7.5 36.0 86-87 29.014300505457832 34.0 29.0 35.0 2.0 35.5 88-89 28.337033874602852 33.0 28.5 35.0 2.0 35.0 90-91 27.777712747827003 33.0 27.0 35.0 2.0 35.0 92-93 27.323600298134785 33.0 26.0 34.5 2.0 35.0 94-95 26.779940866317332 32.5 25.5 34.0 2.0 35.0 96-97 25.96724784204973 32.0 24.5 34.0 2.0 35.0 98-99 24.727017996113986 32.0 11.0 34.0 2.0 35.0 100-101 18.860177302393218 24.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15306939323497204 1101 2 0.17138010088612532 1101 3 0.16431419178091033 1101 4 0.13003720433293608 1101 5 0.1557883412562191 1101 6 0.1080459009080812 1101 7 0.11824297240138293 1101 8 0.15854367345831122 1101 9 0.1949407691457523 1101 10-11 0.21114572185002345 1101 12-13 0.17761687058478515 1101 14-15 0.16061391124588908 1101 16-17 0.16311794400073865 1101 18-19 0.23910022291504163 1101 20-21 0.2818613595848518 1101 22-23 0.30855829721296146 1101 24-25 0.27532980789368366 1101 26-27 0.3461833835141306 1101 28-29 0.3513789426210394 1101 30-31 0.2510110003424515 1101 32-33 0.37618128754426294 1101 34-35 0.40760220025158134 1101 36-37 0.3720242923634913 1101 38-39 0.2447451483876506 1101 40-41 0.3176502491983868 1101 42-43 0.2650264003179572 1101 44-45 0.2053845713091249 1101 46-47 0.26270816006916675 1101 48-49 0.14737273683160623 1101 50-51 0.08198905287640912 1101 52-53 -0.010815065132732116 1101 54-55 0.03407660230465126 1101 56-57 -0.050099861397733036 1101 58-59 -0.043961812958819735 1101 60-61 -0.04136019511459921 1101 62-63 -0.21873652911840935 1101 64-65 -0.07977659788262059 1101 66-67 0.05424718999807965 1101 68-69 0.07927543867944564 1101 70-71 -0.029424728818050028 1101 72-73 -0.005256436444199153 1101 74-75 -0.22916593141104258 1101 76-77 0.05684510647865082 1101 78-79 -0.03133219006667787 1101 80-81 0.22695702686556274 1101 82-83 0.06143920519740931 1101 84-85 0.2422349469252829 1101 86-87 0.22813285787694326 1101 88-89 0.18482403797374403 1101 90-91 0.47045172134599156 1101 92-93 0.33055293560373045 1101 94-95 -0.02700074479215786 1101 96-97 -0.057120413783170676 1101 98-99 0.17297561466891054 1101 100-101 -0.7103794691015359 1102 1 0.03338287585273747 1102 2 0.10984986197225766 1102 3 0.10041740521651121 1102 4 0.10721384043743143 1102 5 0.03290877529776992 1102 6 0.049950232507598 1102 7 0.12704061558807922 1102 8 0.11186749284765796 1102 9 0.14921047292781964 1102 10-11 0.1437895697444631 1102 12-13 0.1952782770322301 1102 14-15 0.14335381691458338 1102 16-17 0.16840479492201155 1102 18-19 0.18104771622956406 1102 20-21 0.19782917380213405 1102 22-23 0.06752537308941697 1102 24-25 0.16616556562973983 1102 26-27 0.12701324554357285 1102 28-29 0.14525695843109077 1102 30-31 0.11112342283502841 1102 32-33 0.14986329406811905 1102 34-35 0.16191699425081651 1102 36-37 0.24269205001137806 1102 38-39 0.193102615548014 1102 40-41 0.19415126671285776 1102 42-43 0.2586908115554323 1102 44-45 0.18474546569332517 1102 46-47 0.2686924859382742 1102 48-49 0.11981581846785616 1102 50-51 0.08252260514065313 1102 52-53 0.10194537449229557 1102 54-55 0.08898151796481812 1102 56-57 0.16702101344431242 1102 58-59 -0.07557042252713586 1102 60-61 0.05918180582578003 1102 62-63 -0.0385607048214851 1102 64-65 0.05196535383502976 1102 66-67 0.11935342291706519 1102 68-69 0.2354372785807115 1102 70-71 0.07260497378854325 1102 72-73 -0.031296725609315956 1102 74-75 -0.2425226455372247 1102 76-77 -0.2327185744993372 1102 78-79 0.20663441562258456 1102 80-81 0.1895270957773718 1102 82-83 0.21569412379625064 1102 84-85 0.07271731123219283 1102 86-87 0.3147189315200123 1102 88-89 0.15841917006695638 1102 90-91 0.19361018545013664 1102 92-93 0.1673782353480675 1102 94-95 0.013320562034369487 1102 96-97 -0.11561601679824918 1102 98-99 0.047688635180829664 1102 100-101 -0.3327291561331869 1103 1 0.1330769969044212 1103 2 0.13358068375359267 1103 3 0.1520263237405146 1103 4 0.06529247232000301 1103 5 0.1158539780152239 1103 6 0.10234163895773918 1103 7 0.10430784396187676 1103 8 0.10765676490557041 1103 9 0.16072244478553444 1103 10-11 0.1688748458064424 1103 12-13 0.11514961573593752 1103 14-15 0.07735056005691376 1103 16-17 0.2566788619852929 1103 18-19 0.2700179870255397 1103 20-21 0.29726800038454115 1103 22-23 0.23817045269622383 1103 24-25 0.18504015881903513 1103 26-27 0.15699107258142675 1103 28-29 0.2651807199712266 1103 30-31 0.17927711307062566 1103 32-33 0.2190507638241712 1103 34-35 0.1830413514187441 1103 36-37 0.11417292990852701 1103 38-39 0.20203792197253279 1103 40-41 0.21756823344689025 1103 42-43 0.16862286349436317 1103 44-45 0.14429582126722096 1103 46-47 0.08678331621163693 1103 48-49 0.02630317412715044 1103 50-51 0.2176142976629336 1103 52-53 0.25228624153280066 1103 54-55 0.13499930970620966 1103 56-57 0.1315487216412521 1103 58-59 0.09368936583642551 1103 60-61 0.10429657862852082 1103 62-63 0.01843771419115825 1103 64-65 0.17259969980678136 1103 66-67 0.15566513242339397 1103 68-69 0.08213813869475217 1103 70-71 0.13156198469059888 1103 72-73 -0.05413386849357238 1103 74-75 -0.01909387773481086 1103 76-77 -0.009849764426562047 1103 78-79 0.09242779729878947 1103 80-81 0.23963730826776342 1103 82-83 0.10359034911482112 1103 84-85 -0.04426077910587267 1103 86-87 -0.1407542735315559 1103 88-89 -0.12057320845913111 1103 90-91 -0.02278983044275762 1103 92-93 0.037133431376265236 1103 94-95 0.3187253920515438 1103 96-97 0.11677592929874336 1103 98-99 -0.07271591611764805 1103 100-101 -0.5532448687516727 1104 1 -0.06985300726341137 1104 2 -0.06279990956268477 1104 3 0.022557713785360534 1104 4 0.013076013958048804 1104 5 0.02710724373574891 1104 6 0.027262383463764195 1104 7 -0.030438089212509567 1104 8 -0.05949363706716326 1104 9 0.027431195554321164 1104 10-11 0.046406340771710575 1104 12-13 0.10384994736561737 1104 14-15 0.04142067819962847 1104 16-17 -0.07693952447777264 1104 18-19 0.052960970023214315 1104 20-21 0.0869442948039989 1104 22-23 0.024523349340334732 1104 24-25 0.14671783053637455 1104 26-27 0.07868565909353009 1104 28-29 -0.019597500868286488 1104 30-31 0.05034484354919044 1104 32-33 -0.049698109440655 1104 34-35 -0.003839161613122144 1104 36-37 0.03917569783673258 1104 38-39 0.09934908985274404 1104 40-41 0.11166498166744532 1104 42-43 0.06776912429869952 1104 44-45 -0.04403778161304217 1104 46-47 -0.11408784209240963 1104 48-49 0.0017235369199326556 1104 50-51 -0.016138688366424958 1104 52-53 -0.14136285589073339 1104 54-55 -0.1524836552173312 1104 56-57 -0.024098112747367395 1104 58-59 0.026784918196057106 1104 60-61 -0.12802027292414664 1104 62-63 -0.208188074088703 1104 64-65 -0.21024853599404025 1104 66-67 -0.17817294928353533 1104 68-69 -0.26485006558356616 1104 70-71 -0.426162795715598 1104 72-73 -0.5063982656330097 1104 74-75 -0.6936045051299367 1104 76-77 -0.5149469549359615 1104 78-79 -0.12814709704170824 1104 80-81 -0.06422512541064762 1104 82-83 -0.080184867290793 1104 84-85 -0.46467356849928976 1104 86-87 -0.5189281886920938 1104 88-89 -0.23535339438275216 1104 90-91 -0.2375514958834195 1104 92-93 -0.3801684096003193 1104 94-95 -0.21719115737669625 1104 96-97 -0.6676826030790615 1104 98-99 -0.8009153657781631 1104 100-101 -0.9388772635399683 1105 1 0.02969594588871871 1105 2 0.10468467356403721 1105 3 0.07925721638991234 1105 4 0.044420106300819384 1105 5 0.041261458476306245 1105 6 0.05842755658089516 1105 7 0.07654623575933073 1105 8 0.021173373806014695 1105 9 0.12941174618249818 1105 10-11 0.10691852687497061 1105 12-13 0.12338750080709104 1105 14-15 0.15153566362197068 1105 16-17 0.16418182194904318 1105 18-19 0.21614850974754773 1105 20-21 0.194985095203414 1105 22-23 0.1719966690758099 1105 24-25 0.10764187165712258 1105 26-27 0.1479359193784049 1105 28-29 0.1713949385164355 1105 30-31 0.2766670140268346 1105 32-33 0.2869473595577503 1105 34-35 0.350072012659318 1105 36-37 0.299982845063397 1105 38-39 0.2030834748903203 1105 40-41 0.21024606700850512 1105 42-43 0.12440898716138094 1105 44-45 0.17541090861303132 1105 46-47 0.1463278128286376 1105 48-49 0.20908964143943365 1105 50-51 0.13583317189936395 1105 52-53 0.20408117902748302 1105 54-55 0.0702422388249957 1105 56-57 0.0642428289130379 1105 58-59 0.0072290468338707115 1105 60-61 -0.16722445724844448 1105 62-63 -0.020032356426646913 1105 64-65 0.0561059592237001 1105 66-67 0.18001780246689236 1105 68-69 0.035620491979862834 1105 70-71 -0.038709804612139465 1105 72-73 -0.08286493468526857 1105 74-75 -0.08888095237347571 1105 76-77 -0.009457968692291985 1105 78-79 -0.14063821498015372 1105 80-81 0.1790594396537557 1105 82-83 0.1180972525441959 1105 84-85 0.01963990139201499 1105 86-87 0.07962179878356324 1105 88-89 0.09990332062618634 1105 90-91 0.16998986427757146 1105 92-93 0.1897242559042276 1105 94-95 0.12675336403141557 1105 96-97 0.18341187138405246 1105 98-99 0.35704602856492684 1105 100-101 -0.8076144516454171 1106 1 0.015224799125654442 1106 2 0.10050011311879814 1106 3 0.05050430555865404 1106 4 0.05289202708662799 1106 5 0.06726625873665881 1106 6 0.06552404775109721 1106 7 0.0970941207202003 1106 8 0.11054578869099885 1106 9 0.11837450073593203 1106 10-11 0.10036214745429817 1106 12-13 0.1788172593427788 1106 14-15 0.16962638370499405 1106 16-17 0.1101838405651634 1106 18-19 0.09431798994290119 1106 20-21 0.12405715298245212 1106 22-23 0.20094583425269974 1106 24-25 0.16701637002662295 1106 26-27 0.2569326693624632 1106 28-29 0.20322085156641378 1106 30-31 0.10945707465128862 1106 32-33 0.21266357797289714 1106 34-35 0.09316449188494147 1106 36-37 0.09252166164419862 1106 38-39 0.23528567953577806 1106 40-41 0.19373610146570996 1106 42-43 0.3128670327099812 1106 44-45 0.2668242037729698 1106 46-47 0.2800429900512569 1106 48-49 0.20444014510395192 1106 50-51 0.13346849944072403 1106 52-53 0.2690397182354758 1106 54-55 0.2623329138300008 1106 56-57 0.1910732992417934 1106 58-59 0.14333995637656471 1106 60-61 0.18677289194356206 1106 62-63 0.31671041514648124 1106 64-65 0.25484921675997896 1106 66-67 0.1753927692081021 1106 68-69 0.09527521626724678 1106 70-71 0.12572110176408557 1106 72-73 0.11426362235602738 1106 74-75 0.07669848171035554 1106 76-77 0.10856318279847343 1106 78-79 0.3896017210750138 1106 80-81 0.5759481211104145 1106 82-83 0.3978288238763277 1106 84-85 0.11468994899881224 1106 86-87 0.2808459971092958 1106 88-89 0.22836484597873152 1106 90-91 0.528104844036438 1106 92-93 0.8652505418924328 1106 94-95 0.6978563959136785 1106 96-97 0.3014180426761648 1106 98-99 0.41729831160217046 1106 100-101 -0.11391773442538877 1107 1 0.05016388523540627 1107 2 0.004764834421429498 1107 3 0.013751337972593092 1107 4 -0.01932458369147838 1107 5 0.06327604048239266 1107 6 0.04582948388513586 1107 7 0.03516268067860295 1107 8 0.06884844930016243 1107 9 0.07129088831647579 1107 10-11 0.08399601998559802 1107 12-13 0.021721641761743626 1107 14-15 0.16528053126363318 1107 16-17 0.05721668249292122 1107 18-19 0.16048440587787383 1107 20-21 0.09739956865207233 1107 22-23 0.14437151031876994 1107 24-25 0.1796446604787718 1107 26-27 0.07826307024070189 1107 28-29 0.026226304178123883 1107 30-31 0.16025926446980066 1107 32-33 0.12483343602951891 1107 34-35 0.0816029702467631 1107 36-37 0.17930549811720908 1107 38-39 0.11445297927321008 1107 40-41 0.22250127643933837 1107 42-43 0.19336645599143054 1107 44-45 0.14233164119526265 1107 46-47 0.12882128831425632 1107 48-49 0.10874699681986755 1107 50-51 0.1164106307099928 1107 52-53 0.2710063391783777 1107 54-55 0.10548472883495918 1107 56-57 -0.09626213802641814 1107 58-59 0.053115603232214426 1107 60-61 0.0450091336218037 1107 62-63 0.04276696566339666 1107 64-65 0.015422479274988632 1107 66-67 -0.06076745689981067 1107 68-69 -0.0604216715925574 1107 70-71 -0.2457792652386246 1107 72-73 -0.32306518104393334 1107 74-75 -0.3172255637081065 1107 76-77 -0.4226162654776857 1107 78-79 -0.221768354615282 1107 80-81 -0.09927775140376127 1107 82-83 -0.1340157499404384 1107 84-85 -0.18486156940803156 1107 86-87 -0.30455997422811265 1107 88-89 -0.2942583834739345 1107 90-91 -0.0556774345475084 1107 92-93 0.06547820662101245 1107 94-95 -0.20576647188616803 1107 96-97 -0.34986745654381934 1107 98-99 -0.21156297421269343 1107 100-101 -1.1376153562906204 1108 1 -0.05673993116480602 1108 2 -0.035518559080372825 1108 3 -0.008086980748402084 1108 4 0.01770945447252359 1108 5 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-0.8124932605160282 2316 92-93 -0.8387206437169112 2316 94-95 -0.45413852722984416 2316 96-97 -0.6115728956822863 2316 98-99 -0.5152027047566783 2316 100-101 0.5084483736691041 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 16.0 5 38.0 6 57.0 7 177.0 8 520.0 9 972.0 10 1563.0 11 2427.0 12 3702.0 13 5207.0 14 6403.0 15 8950.0 16 11417.0 17 16188.0 18 16323.0 19 12380.0 20 11956.0 21 12422.0 22 13254.0 23 14263.0 24 16097.0 25 18663.0 26 21623.0 27 26038.0 28 31518.0 29 38132.0 30 46574.0 31 57732.0 32 73131.0 33 96305.0 34 137099.0 35 210942.0 36 346225.0 37 489060.0 38 269126.0 39 10765.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.04282565080623 18.559532360736135 14.622445693904343 23.77519629455329 2 16.12108100002417 19.76908859249701 39.21513206670445 24.894698340774372 3 19.785859907234897 23.301078148900665 28.946401344467514 27.966660599396924 4 13.877276397679442 16.07352939000262 34.98698496648943 35.062209245828505 5 14.783420667488462 36.41303999317309 33.196597790823226 15.606941548515222 6 33.99471506276171 34.42065712970786 16.52349389894099 15.061133908589428 7 29.959536736825022 31.774488955842607 19.75651010644359 18.509464200888782 8 27.037825727295463 34.5654330378129 19.093697882522857 19.303043352368775 9 26.772247025681324 16.82774527924486 19.69263112825074 36.707376566823086 10-11 25.942288919438987 25.824100477933143 26.071871989487345 22.16173861314052 12-13 26.578538340458675 23.033649669925726 26.93219110814489 23.455620881470708 14-15 23.27202789389684 24.444377577841152 25.063565064753867 27.220029463508137 16-17 23.134643103432968 27.620378783618598 25.973991059704243 23.270987053244188 18-19 23.232740771396813 26.07276631312891 28.106609354795033 22.58788356067925 20-21 23.58456269536732 25.249855594047364 28.365102642912564 22.80047906767275 22-23 23.35960510459628 25.05777471290222 28.401111642202743 23.181508540298758 24-25 23.329822865232554 25.3628525060796 28.362921564468476 22.94440306421937 26-27 22.806506511444358 25.72365824782952 28.230736977111288 23.23909826361484 28-29 22.940327894082216 26.05286270381777 28.1716722065457 22.835137195554317 30-31 23.03648599521228 25.554624912012258 28.023165131844728 23.38572396093073 32-33 22.779964489198655 25.623072993959116 28.07301738963794 23.52394512720429 34-35 23.45232207497487 25.701494194041725 27.856118302181166 22.99006542880224 36-37 23.55748652448536 25.382025196726847 27.801191228339817 23.259297050447973 38-39 23.057474706634455 25.69331808154019 27.891860609399092 23.35734660242626 40-41 23.372182222255113 25.552258538739125 27.984840300234083 23.090718938771683 42-43 23.25097769824366 25.556236610991412 27.6102230668954 23.582562623869528 44-45 23.17837296546689 25.828599757792208 27.776645940059737 23.21638133668116 46-47 23.661575800627478 26.055960062353243 27.153283411274888 23.12918072574439 48-49 23.440931450411504 26.18352667023151 26.950075078778458 23.425466800578533 50-51 23.467595344443595 26.311268192703928 27.025879822715936 23.195256640136538 52-53 23.890614525635694 26.009596151673854 26.87975608589084 23.22003323679962 54-55 23.42188094240977 26.22656270424625 26.921289753565258 23.43026659977872 56-57 23.351796605086253 26.47064916922676 26.824647752541846 23.352906473145136 58-59 23.367452818205653 25.9824386721148 26.93417671379071 23.715931795888842 60-61 23.13049582137526 26.181148389273595 27.23014548973916 23.45821029961198 62-63 23.136445050193835 26.26167781728961 27.318014977005696 23.283862155510864 64-65 22.97127413009329 26.32316412496063 27.1401225671975 23.56543917774858 66-67 22.967279554308977 26.363187476137433 27.007143723911497 23.66238924564209 68-69 23.22314169853153 26.02861462015839 27.19624621487825 23.551997466431825 70-71 23.222260916919634 26.14072351630225 26.827411893653817 23.809603673124297 72-73 23.102091432275213 26.219336240690073 26.841478026371412 23.837094300663306 74-75 23.00156219864044 26.59464373534668 26.624782774478597 23.779011291534285 76-77 23.154128712609744 26.25644314785313 26.821541684596017 23.767886454941106 78-79 23.095104949269977 26.35126918178519 26.87348094223085 23.680144926713982 80-81 23.234997136533345 26.355534531040124 26.70314606442282 23.706322268003706 82-83 23.20116550771949 26.3902556688277 26.740973456434787 23.667605367018027 84-85 22.943701162794714 26.487184002533454 26.659780562144874 23.90933427252696 86-87 23.199771855795085 26.32460673519223 26.5809479836151 23.894673425397585 88-89 22.98553377413566 26.41618790613115 26.41715388988033 24.18112442985286 90-91 22.898689867203284 26.333565180171746 26.548147355509382 24.219597597115587 92-93 22.91869217287674 26.10104914439165 26.68787251433084 24.29238616840077 94-95 22.749301894024036 25.846445788451568 26.92565522813674 24.478597089387655 96-97 22.95366529684487 25.59938952414354 26.85815068631673 24.58879449269486 98-99 22.634121003943985 25.89916116027287 26.624300874404895 24.84241696137825 100-101 23.148711761270363 24.705440435976765 27.299873129498675 24.845974673254197 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 211.0 1 167.5 2 160.0 3 224.5 4 218.0 5 225.5 6 399.0 7 830.0 8 1384.0 9 1647.5 10 1596.5 11 1522.0 12 1554.5 13 1675.0 14 1811.5 15 1962.0 16 2116.0 17 2310.0 18 2491.0 19 2690.0 20 3086.0 21 3642.5 22 4447.5 23 5516.5 24 6608.5 25 8069.5 26 10380.5 27 13043.5 28 15189.0 29 17435.5 30 19851.0 31 23115.0 32 28643.0 33 30818.5 34 30693.5 35 32786.0 36 35682.5 37 38938.0 38 41776.0 39 44241.5 40 47587.0 41 51806.0 42 54583.5 43 57230.5 44 60394.5 45 64328.0 46 67376.5 47 70408.0 48 74318.0 49 76175.0 50 76014.0 51 75674.0 52 77478.0 53 81066.5 54 83359.5 55 79127.0 56 73343.0 57 68404.0 58 63486.0 59 57658.5 60 48417.5 61 40848.0 62 34697.0 63 26022.0 64 18486.5 65 14091.5 66 10634.5 67 8617.5 68 6715.5 69 5086.0 70 3918.5 71 2737.5 72 2091.0 73 1662.0 74 1268.0 75 1021.0 76 751.5 77 481.0 78 313.0 79 209.0 80 131.0 81 74.5 82 49.0 83 28.0 84 17.0 85 13.0 86 9.5 87 4.5 88 1.5 89 1.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.42525148339812485 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.632294350385322E-4 16-17 0.002219732832956225 18-19 2.466369814395806E-5 20-21 0.0 22-23 0.0 24-25 4.932739628791612E-5 26-27 0.0035022451364420445 28-29 2.466369814395806E-5 30-31 0.0 32-33 2.466369814395806E-5 34-35 0.012751131940426317 36-37 0.026612130297330748 38-39 0.020002259194749985 40-41 0.009174895709552398 42-43 0.009298214200272189 44-45 0.0042421560807607865 46-47 0.005475340987958689 48-49 0.00330493555129038 50-51 7.892383406066579E-4 52-53 0.0 54-55 0.0 56-57 1.4798218886374835E-4 58-59 0.001603140379357274 60-61 0.0024170424181078898 62-63 0.0014798218886374836 64-65 0.0027869978902672608 66-67 0.0018251136626528963 68-69 0.004464129364056409 70-71 2.466369814395806E-4 72-73 0.0 74-75 0.0 76-77 0.09670636042245954 78-79 0.015562793528837535 80-81 3.9461917030332897E-4 82-83 0.0 84-85 0.0 86-87 0.11029605809978044 88-89 0.4243882539630863 90-91 0.42900036551600645 92-93 0.0 94-95 0.0 96-97 0.0 98-99 3.206280758714548E-4 100-101 3.452917740154128E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2027271.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.06584524534236 #Duplication Level Percentage of deduplicated Percentage of total 1 74.52678644257735 39.54826915994488 2 13.981569469918481 14.83887603555395 3 4.831390054790672 7.691453909022237 4 2.1882942851440386 4.644947435468826 5 1.158056862090827 3.0726633114509303 6 0.7309323112694751 2.327252454878783 7 0.508255006635572 1.88796870691056 8 0.33417779922504603 1.4186741902484308 9 0.24715427050738611 1.1803905243423392 >10 1.3589202030342538 13.088224242197144 >50 0.08644920322537633 3.1455150279754998 >100 0.04290821081927404 4.073215621301942 >500 0.0035276994358577796 1.3747402716497812 >1k 0.0014853471308874805 1.4156780329104728 >5k 9.283419568046753E-5 0.29213107614431233 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5930 0.2925114599873426 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4607 0.22725131469842957 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3041 0.15000461211155292 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2115 0.10432744314894259 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2035 0.10038125144590931 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.4798218886374835E-4 2 0.0 0.0 0.0 0.0 1.4798218886374835E-4 3 0.0 0.0 0.0 0.0 1.4798218886374835E-4 4 0.0 0.0 0.0 0.0 1.4798218886374835E-4 5 0.0 0.0 0.0 0.0 1.4798218886374835E-4 6 0.0 0.0 0.0 0.0 1.9730958515166449E-4 7 0.0 0.0 0.0 0.0 1.9730958515166449E-4 8 0.0 0.0 0.0 0.0 1.9730958515166449E-4 9 0.0 0.0 0.0 0.0 1.9730958515166449E-4 10-11 0.0 0.0 0.0 0.0 1.9730958515166449E-4 12-13 0.0 0.0 0.0 0.0 6.905835480308256E-4 14-15 0.0 0.0 0.0 0.0 9.865479257583224E-4 16-17 0.0 0.0 0.0 0.0 0.0010852027183341547 18-19 0.0 0.0 0.0 0.0 0.0011345301146220706 20-21 0.0 0.0 0.0 0.0 0.0013318396997737353 22-23 0.0 0.0 0.0 4.932739628791612E-5 0.0014304944923495674 24-25 0.0 0.0 0.0 2.466369814395806E-4 0.0014304944923495674 26-27 0.0 0.0 0.0 5.919287554549934E-4 0.00165246777564519 28-29 0.0 0.0 0.0 0.00254036090882768 0.0017264588700770642 30-31 0.0 0.0 0.0 0.007226463556179712 0.0017264588700770642 32-33 0.0 0.0 0.0 0.015932749000996907 0.0018744410589408126 34-35 0.0 0.0 0.0 0.03184083430384985 0.002022423247804561 36-37 0.0 0.0 0.0 0.05731843448655853 0.002071750644092477 38-39 0.0 0.0 0.0 0.09687900630946726 0.0021950691348122673 40-41 0.0 0.0 0.0 0.13875796575790805 0.0022197328329562256 42-43 0.0 0.0 0.0 0.18482975389082168 0.0022197328329562256 44-45 0.0 0.0 0.0 0.24338137328457812 0.0022690602292441417 46-47 0.0 0.0 0.0 0.3119464541247815 0.002392378719963932 48-49 0.0 0.0 0.0 0.3860115396510876 0.0024910335125397642 50-51 0.0 0.0 0.0 0.4718658728902056 0.002515697210683722 52-53 0.0 0.0 0.0 0.558657426658794 0.0027130067958353868 54-55 0.0 0.0 0.0 0.6549445042128063 0.002762334192123303 56-57 0.0 0.0 0.0 0.7649199342367152 0.002811661588411219 58-59 0.0 0.0 0.0 0.8929245276038575 0.002860988984699135 60-61 0.0 0.0 0.0 1.0363439323109738 0.002910316380987051 62-63 0.0 0.0 0.0 1.1863978718188144 0.0031076259661387156 64-65 0.0 0.0 0.0 1.3412119050684392 0.0032309444568585062 66-67 0.0 0.0 0.0 1.5298152047752867 0.003255608155002464 68-69 0.0 0.0 0.0 1.7432795122112437 0.003255608155002464 70-71 0.0 0.0 0.0 1.9772393528048298 0.003329599249434338 72-73 0.0 0.0 0.0 2.2174144453306934 0.0035515725327299606 74-75 0.0 0.0 0.0 2.471450536213461 0.003600899929017877 76-77 0.0 0.0 0.0 2.754491136113524 0.0036748910234497513 78-79 0.0 0.0 0.0 3.0741080003610763 0.003773545816025583 80-81 0.0 0.0 0.0 3.4287226522749057 0.003872200608601415 82-83 0.0 0.0 0.0 3.7972476299419267 0.0040941738918970376 84-85 0.0 0.0 0.0 4.173640327316871 0.0040941738918970376 86-87 0.0 0.0 0.0 4.5741294577784615 0.004266819778904744 88-89 0.0 0.0 0.0 5.013759877194515 0.004439465665912451 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4330 0.0 43.039547 1 GTATCAA 6755 0.0 35.63238 1 ATCAACG 8725 0.0 27.463865 3 TATCAAC 8750 0.0 27.33117 2 TCAACGC 8780 0.0 27.291824 4 CAACGCA 8890 0.0 26.95413 5 AACGCAG 9225 0.0 25.923872 6 ACGCAGA 10570 0.0 22.490463 7 CGCAGAG 10730 0.0 22.1551 8 TACATGG 7455 0.0 22.149673 2 GTACATG 7855 0.0 22.08686 1 ACATGGG 7505 0.0 21.749208 3 GTGGTAT 2415 0.0 21.117643 1 GAGTACT 6515 0.0 20.72117 12-13 TGGTATC 2520 0.0 19.959105 2 GTACTTT 6825 0.0 19.640938 14-15 AGTACTT 6825 0.0 19.640938 12-13 TAAGGTG 1065 0.0 19.158195 5 AGAGTAC 10070 0.0 19.084114 10-11 CATGGGG 4855 0.0 19.058165 4 >>END_MODULE