##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939075_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1069891 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.37713561474954 34.0 31.0 34.0 31.0 34.0 2 32.57258449692539 34.0 31.0 34.0 31.0 34.0 3 32.63686581156398 34.0 31.0 34.0 31.0 34.0 4 36.149915271742636 37.0 35.0 37.0 35.0 37.0 5 36.12691105916397 37.0 35.0 37.0 35.0 37.0 6 36.1901118899028 37.0 37.0 37.0 35.0 37.0 7 36.14403803751971 37.0 36.0 37.0 35.0 37.0 8 36.152949225668785 37.0 37.0 37.0 35.0 37.0 9 37.83767505287922 39.0 38.0 39.0 35.0 39.0 10-11 37.79601940758451 39.0 38.0 39.0 35.0 39.0 12-13 37.74047169291077 39.0 38.0 39.0 35.0 39.0 14-15 38.97115173414862 40.0 38.0 41.0 35.5 41.0 16-17 38.948723748494004 40.0 38.0 41.0 35.5 41.0 18-19 39.06017715823388 40.5 38.5 41.0 35.0 41.0 20-21 39.14196305978834 41.0 39.0 41.0 35.0 41.0 22-23 39.064640229705645 41.0 39.0 41.0 35.0 41.0 24-25 38.98672060985652 41.0 38.0 41.0 35.0 41.0 26-27 38.7913488383396 40.0 38.0 41.0 35.0 41.0 28-29 38.60446391267895 40.0 38.0 41.0 35.0 41.0 30-31 38.2811664926614 40.0 37.5 41.0 34.0 41.0 32-33 37.93201410237118 40.0 36.5 41.0 33.5 41.0 34-35 37.73298775295801 40.0 36.0 41.0 33.0 41.0 36-37 37.528573938840495 40.0 35.5 41.0 33.0 41.0 38-39 37.3077154588645 40.0 35.0 41.0 33.0 41.0 40-41 37.088768855892795 40.0 35.0 41.0 33.0 41.0 42-43 36.78649273617593 40.0 35.0 41.0 31.5 41.0 44-45 36.3403898154111 40.0 35.0 41.0 30.5 41.0 46-47 35.7337088544534 40.0 35.0 41.0 28.0 41.0 48-49 35.1627390079924 40.0 35.0 41.0 23.5 41.0 50-51 34.82917745826444 39.0 35.0 41.0 22.5 41.0 52-53 34.611078137866386 39.0 35.0 41.0 20.0 41.0 54-55 34.37514709442364 39.0 35.0 41.0 18.5 41.0 56-57 34.09353803331368 39.0 35.0 40.5 14.0 41.0 58-59 33.81016664314402 38.0 34.0 40.0 8.5 41.0 60-61 33.4356177404988 38.0 34.0 40.0 4.0 41.0 62-63 33.069050024722145 38.0 33.5 40.0 2.0 41.0 64-65 32.67716664594805 37.0 33.0 40.0 2.0 41.0 66-67 32.295746482585606 37.0 33.0 39.5 2.0 41.0 68-69 31.804152011746993 36.0 32.0 39.0 2.0 40.0 70-71 31.388616223521836 35.5 32.0 39.0 2.0 40.0 72-73 30.925716264554055 35.0 31.0 38.0 2.0 40.0 74-75 30.422304234730454 35.0 31.0 37.0 2.0 39.5 76-77 28.457338177440505 33.5 28.0 35.5 2.0 37.5 78-79 29.34834576606402 34.5 29.5 36.0 2.0 39.0 80-81 29.299413678589687 35.0 30.0 36.0 2.0 38.0 82-83 28.97372255678382 34.5 29.5 35.5 2.0 37.0 84-85 28.56708113256397 34.0 29.0 35.0 2.0 37.0 86-87 28.027557947491847 34.0 29.0 35.0 2.0 36.0 88-89 27.446146850473554 34.0 26.5 35.0 2.0 36.0 90-91 26.97407399445364 34.0 26.0 35.0 2.0 35.5 92-93 26.69821925784963 34.0 25.5 35.0 2.0 35.0 94-95 26.35728873315132 33.0 25.0 35.0 2.0 35.0 96-97 25.8205536825714 33.0 23.5 35.0 2.0 35.0 98-99 25.151349997336176 33.0 10.5 35.0 2.0 35.0 100-101 21.92255239085103 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17475973303969994 1101 2 0.2544676082658768 1101 3 0.3137668807223122 1101 4 0.19818328989313017 1101 5 0.21237633006806078 1101 6 0.18659171514183726 1101 7 0.16736253866567097 1101 8 0.16208943948090848 1101 9 0.21362162392573936 1101 10-11 0.3053263863041167 1101 12-13 0.27848610650561767 1101 14-15 0.4492039676087245 1101 16-17 0.3585390934241488 1101 18-19 0.34282762667492506 1101 20-21 0.46685852328376853 1101 22-23 0.39271906821974056 1101 24-25 0.43427037563488113 1101 26-27 0.4371848448597788 1101 28-29 0.33648570446268167 1101 30-31 0.43404132390534755 1101 32-33 0.41580450969046723 1101 34-35 0.5378399422983691 1101 36-37 0.7716628735188635 1101 38-39 0.8408160611461355 1101 40-41 0.7285133867099134 1101 42-43 0.8176561262754944 1101 44-45 0.8073741407191406 1101 46-47 0.8382510826993723 1101 48-49 0.5977158577637596 1101 50-51 0.5496653949524344 1101 52-53 0.7684622726665395 1101 54-55 0.8284122740745019 1101 56-57 0.9266030977229036 1101 58-59 1.03651829276739 1101 60-61 0.9871337283625152 1101 62-63 0.9135506180438426 1101 64-65 0.8537422642895791 1101 66-67 0.945716743927072 1101 68-69 0.8176833989221599 1101 70-71 0.7330381947551778 1101 72-73 0.325142976757423 1101 74-75 0.5369011347572012 1101 76-77 0.7059516458056017 1101 78-79 0.7511457220401354 1101 80-81 0.7594572956102148 1101 82-83 0.6769736536821753 1101 84-85 0.8193247639195782 1101 86-87 0.7657722046503679 1101 88-89 0.8443386202567709 1101 90-91 0.921221941246138 1101 92-93 0.8012753514341355 1101 94-95 0.6526619322505312 1101 96-97 0.6922373330733613 1101 98-99 0.631409322462023 1101 100-101 0.32447440796847715 1102 1 0.2240010539752646 1102 2 0.11947128029744647 1102 3 0.3150103924292509 1102 4 0.25913154536694094 1102 5 0.21877188877700604 1102 6 0.2079069248210601 1102 7 0.21406953544175167 1102 8 0.2057019716889812 1102 9 0.35279659934180074 1102 10-11 0.23744172543801767 1102 12-13 0.330356975470103 1102 14-15 0.3150665216000377 1102 16-17 0.39973555333676813 1102 18-19 0.2706124580726623 1102 20-21 0.3577076961430876 1102 22-23 0.3934559640460691 1102 24-25 0.28859465958872477 1102 26-27 0.3734085027011318 1102 28-29 0.26164098783149115 1102 30-31 0.40054990650116196 1102 32-33 0.4207493047413209 1102 34-35 0.4691374764358116 1102 36-37 0.6012333080633425 1102 38-39 0.6846856355639019 1102 40-41 0.780159577132757 1102 42-43 0.624955378176864 1102 44-45 0.7550227957597784 1102 46-47 0.7760611826533221 1102 48-49 0.8758068992514936 1102 50-51 0.9472406093617352 1102 52-53 0.980848221855581 1102 54-55 1.1771227063784195 1102 56-57 1.023435814901788 1102 58-59 0.9382870917018806 1102 60-61 1.2172406529427278 1102 62-63 1.1298855571130133 1102 64-65 0.9756089010820901 1102 66-67 1.1164861278970974 1102 68-69 0.9513733549822554 1102 70-71 0.8833917055907854 1102 72-73 0.9310692622796601 1102 74-75 1.0711263360965617 1102 76-77 0.8739570480486556 1102 78-79 0.8709504197510292 1102 80-81 0.8036175206621863 1102 82-83 0.9085052020347391 1102 84-85 0.8799823687591903 1102 86-87 0.9050175088066119 1102 88-89 0.9329086440316168 1102 90-91 1.014637179554768 1102 92-93 0.75663651752199 1102 94-95 0.7932920279723028 1102 96-97 0.6863166489552093 1102 98-99 0.5300058595495436 1102 100-101 0.19272938388244043 1103 1 0.1296718099554326 1103 2 0.22261968980781432 1103 3 0.2065494942278221 1103 4 0.17167813820883282 1103 5 0.20360162134313953 1103 6 0.18110563650636635 1103 7 0.23879721611341154 1103 8 0.2060710955626064 1103 9 0.23347911662186505 1103 10-11 0.22144997644224418 1103 12-13 0.2783050809961054 1103 14-15 0.35312970692192636 1103 16-17 0.37254342797940865 1103 18-19 0.47675004718713154 1103 20-21 0.41711673605913546 1103 22-23 0.37562221454366096 1103 24-25 0.254680498983646 1103 26-27 0.3714235571649951 1103 28-29 0.5066270552146861 1103 30-31 0.4666900288186824 1103 32-33 0.5311969579096285 1103 34-35 0.6471366079090544 1103 36-37 0.6566815040608844 1103 38-39 0.6344721227350405 1103 40-41 0.5758875111247193 1103 42-43 0.6937227939478561 1103 44-45 0.8581693958393899 1103 46-47 0.8555440473440896 1103 48-49 0.7359740906530803 1103 50-51 0.8549686881081655 1103 52-53 0.7617153010724635 1103 54-55 0.7578902669038783 1103 56-57 0.7577842388876093 1103 58-59 0.8719045056634442 1103 60-61 0.9572381197396709 1103 62-63 0.6267986682482487 1103 64-65 0.7187927754352188 1103 66-67 0.6089263262107139 1103 68-69 0.9366988715747375 1103 70-71 0.7778910361014049 1103 72-73 0.7693984339222624 1103 74-75 0.666856079519281 1103 76-77 0.6381990775061865 1103 78-79 0.8179793344544599 1103 80-81 0.7953556598435974 1103 82-83 0.6451986482765513 1103 84-85 0.6173543515334075 1103 86-87 0.6716389397044509 1103 88-89 0.8362741852323552 1103 90-91 0.6890102117988199 1103 92-93 0.7359854843370286 1103 94-95 0.7336369054738405 1103 96-97 0.5079646784851164 1103 98-99 0.20944891676374056 1103 100-101 0.30324036712711333 1104 1 0.08585247352079506 1104 2 0.08265891349779508 1104 3 0.10859935429402157 1104 4 0.040339770543042164 1104 5 0.14182665553484242 1104 6 0.06152968400644454 1104 7 0.10479037073498176 1104 8 0.09238338838903815 1104 9 0.13655414131261523 1104 10-11 0.2502179256203618 1104 12-13 0.2416964991717805 1104 14-15 0.24107710213579736 1104 16-17 0.12267069383954521 1104 18-19 0.10597943774200758 1104 20-21 0.16542533172435725 1104 22-23 0.1054162667121119 1104 24-25 0.09198136016885172 1104 26-27 0.15723863341894173 1104 28-29 0.0714305966071791 1104 30-31 0.24321176488923157 1104 32-33 0.15262970171465895 1104 34-35 0.23688484436784307 1104 36-37 0.23192905668560826 1104 38-39 0.30055876204834675 1104 40-41 0.3711777359337063 1104 42-43 0.4140821645859276 1104 44-45 0.5316527034849656 1104 46-47 0.47852920005139055 1104 48-49 0.45195539840656096 1104 50-51 0.34587768304802324 1104 52-53 0.3047229510995564 1104 54-55 0.281378149082677 1104 56-57 0.49500135092613107 1104 58-59 0.44970617267026824 1104 60-61 0.3345649736572298 1104 62-63 0.3372553474186404 1104 64-65 0.39933787212208216 1104 66-67 0.2588971195755292 1104 68-69 0.207681317096732 1104 70-71 0.1209845465981978 1104 72-73 0.022262795493368515 1104 74-75 -0.11396161242930702 1104 76-77 0.023976501832851937 1104 78-79 0.14220876019805573 1104 80-81 0.09120949060463701 1104 82-83 0.15994565313024722 1104 84-85 0.01742074091298562 1104 86-87 0.24861953664584746 1104 88-89 0.19177289146052345 1104 90-91 0.3282478599726417 1104 92-93 0.4528764204272804 1104 94-95 0.6225795549027282 1104 96-97 0.640946359238999 1104 98-99 0.4689052980029942 1104 100-101 0.22122191885570075 1105 1 0.06699092977228105 1105 2 0.09563128660045095 1105 3 0.038648352281356324 1105 4 0.0825708479843783 1105 5 0.15980389341421386 1105 6 0.09139361253526346 1105 7 0.14694702639524593 1105 8 0.1019454928181176 1105 9 0.10479245736659237 1105 10-11 0.19090978551916749 1105 12-13 0.17512238547499948 1105 14-15 0.17904709483589443 1105 16-17 0.1814748452616115 1105 18-19 0.18586629474602745 1105 20-21 0.13214675677013332 1105 22-23 0.07520594856993768 1105 24-25 0.05866776320000611 1105 26-27 0.16682974045388477 1105 28-29 0.10920785878307271 1105 30-31 0.22808334901156258 1105 32-33 -6.572785230787304E-4 1105 34-35 0.13947001626574007 1105 36-37 0.0427346615212727 1105 38-39 0.05373618904659594 1105 40-41 0.2585836005889419 1105 42-43 0.10664852309534467 1105 44-45 0.0021625255184076764 1105 46-47 -0.22876117951064145 1105 48-49 -0.2143570351747357 1105 50-51 -0.23149743884500396 1105 52-53 -0.0038264698880468018 1105 54-55 -0.02093461720776446 1105 56-57 -0.019635662308900237 1105 58-59 0.0070005469308469515 1105 60-61 0.022022495784028706 1105 62-63 -0.13912503981539714 1105 64-65 -0.009821588582667573 1105 66-67 -0.36737311247815185 1105 68-69 -0.5834676348147525 1105 70-71 -0.37731524403582384 1105 72-73 -0.45043535523481637 1105 74-75 -0.5060500298829673 1105 76-77 -0.5788503219659198 1105 78-79 -0.3356474766208031 1105 80-81 -0.40485326884556017 1105 82-83 -0.5750602732512888 1105 84-85 -0.6585008579295355 1105 86-87 -0.38578291664627784 1105 88-89 -0.3812753699700622 1105 90-91 -0.48845129326070946 1105 92-93 -0.35644840985275295 1105 94-95 -0.535388336476494 1105 96-97 -0.5315726319956475 1105 98-99 -0.4117177874146023 1105 100-101 -0.22858715644272465 1106 1 0.02277085928596989 1106 2 0.005546464963948949 1106 3 0.04602612683900276 1106 4 0.025476933509843036 1106 5 -0.07765011193694704 1106 6 0.04303337675285235 1106 7 0.006781730295799093 1106 8 -0.019061188373626692 1106 9 0.13784753709065 1106 10-11 0.12382720474712983 1106 12-13 0.09972842287104555 1106 14-15 0.13312127624758574 1106 16-17 0.08475903138273821 1106 18-19 -0.013793176658367656 1106 20-21 0.0819436299102918 1106 22-23 0.0642086345021795 1106 24-25 -0.0025166345870744067 1106 26-27 0.007453042339207627 1106 28-29 0.0654276808594716 1106 30-31 0.18990377969225136 1106 32-33 0.031107104669914065 1106 34-35 -0.1295087602119125 1106 36-37 -0.215280957252304 1106 38-39 -0.15702565311995187 1106 40-41 -0.3373569673382306 1106 42-43 -0.4024329294816482 1106 44-45 -0.24839044530894938 1106 46-47 -0.24559353904258785 1106 48-49 -0.23797512562699552 1106 50-51 -0.2595858727757232 1106 52-53 -0.2257119011638835 1106 54-55 -0.4162924568695061 1106 56-57 -0.34457912317657957 1106 58-59 -0.42744045312608847 1106 60-61 -0.3945423055656647 1106 62-63 -0.27120459309156075 1106 64-65 -0.05576655543546849 1106 66-67 -0.03818339457866671 1106 68-69 -0.31600726395944534 1106 70-71 -0.35823994995470443 1106 72-73 -0.5039500968108754 1106 74-75 -0.34852657325896175 1106 76-77 -0.14991367167015923 1106 78-79 -0.18160745157280545 1106 80-81 -0.10237693247783142 1106 82-83 -0.21467078720105803 1106 84-85 -0.0773287771342126 1106 86-87 -0.21510770743854835 1106 88-89 -0.07724595409563051 1106 90-91 -0.035899657636655036 1106 92-93 0.07967246227306646 1106 94-95 0.09394007696041484 1106 96-97 0.2183044568471253 1106 98-99 0.2762891887639185 1106 100-101 0.04456231905008323 1107 1 -0.05796615913948955 1107 2 -0.06393803842082946 1107 3 -0.10124077968573175 1107 4 -0.04603546080888776 1107 5 0.04637236732325789 1107 6 0.05120242713295653 1107 7 -0.06742559826019345 1107 8 -0.021791803976299207 1107 9 0.06297217408231859 1107 10-11 0.05191289949304689 1107 12-13 -0.028371643535940905 1107 14-15 0.06818515580315676 1107 16-17 -0.05194420868347294 1107 18-19 0.009895285375669971 1107 20-21 0.1140484340096819 1107 22-23 0.09388115885966641 1107 24-25 -0.02453302819218095 1107 26-27 0.12713825340439655 1107 28-29 0.0333065228009346 1107 30-31 -0.017253307518949157 1107 32-33 0.11629041768469506 1107 34-35 0.058940884461158305 1107 36-37 -0.07982854727341504 1107 38-39 0.04506814922510216 1107 40-41 -0.03317828298946779 1107 42-43 0.05657661528360336 1107 44-45 -0.024029488651898134 1107 46-47 0.09003926006140972 1107 48-49 0.011481229125067216 1107 50-51 -0.25534127701585874 1107 52-53 -0.1173976474495575 1107 54-55 -0.17438568434813106 1107 56-57 -0.2729314370140088 1107 58-59 -0.20267064749041452 1107 60-61 -0.14253721105959727 1107 62-63 -0.28356223931082525 1107 64-65 -0.21824759971009655 1107 66-67 -0.11690540700767116 1107 68-69 0.015718425632496036 1107 70-71 0.24573852228816762 1107 72-73 0.0575105595280192 1107 74-75 -4.1471217840793884E-4 1107 76-77 -0.08357358861186981 1107 78-79 0.018297398119443642 1107 80-81 0.2280136793584724 1107 82-83 0.16404081816304128 1107 84-85 0.3236222303687839 1107 86-87 0.2988546090749864 1107 88-89 0.15042246690483552 1107 90-91 0.2436296456846847 1107 92-93 0.37232507444635843 1107 94-95 0.2938077585636947 1107 96-97 0.3263124455083819 1107 98-99 0.47494717296500255 1107 100-101 0.020291061381065845 1108 1 0.05908218691649125 1108 2 0.06092833615194593 1108 3 0.047762863959718516 1108 4 0.10677368444006419 1108 5 0.06217592665294802 1108 6 0.14689140736392403 1108 7 0.04871371848100381 1108 8 0.013645282914112045 1108 9 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-0.8158094135534242 2316 100-101 -0.6921775100807999 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 15.0 5 29.0 6 139.0 7 287.0 8 499.0 9 887.0 10 1627.0 11 2691.0 12 4302.0 13 6276.0 14 7257.0 15 10331.0 16 14217.0 17 21550.0 18 20005.0 19 10820.0 20 8198.0 21 7728.0 22 7850.0 23 8443.0 24 9416.0 25 10410.0 26 11435.0 27 12862.0 28 15163.0 29 17802.0 30 21480.0 31 25477.0 32 31150.0 33 39610.0 34 54383.0 35 87108.0 36 118550.0 37 216361.0 38 238951.0 39 26578.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.83202025886325 23.165447383230166 16.30604014256237 18.69649221534421 2 14.410533409478163 24.481933206279894 40.6383454015409 20.469187982701044 3 18.312893556446404 29.90734570157147 32.944570989007296 18.835189752974834 4 10.904288380778976 20.261970611959537 40.95090060576264 27.882840401498843 5 10.019805755913453 41.31121768479219 35.30266167301155 13.36631488628281 6 24.403887872689833 41.836785242608826 18.687978495005567 15.071348389695775 7 22.560055183191558 38.4802750934441 22.925699907747614 16.03396981561673 8 23.824950392142753 34.848596726208555 21.394422422471074 19.932030459177614 9 25.370902269483526 18.46870382123039 24.17853781366513 31.98185609562096 10-11 22.155481259305855 29.492957693821147 28.496594512898977 19.85496653397402 12-13 24.7068626617104 28.832329648534287 28.916870970968073 17.54393671878724 14-15 21.37686340817343 29.246149805445654 30.443619430572184 18.93336735580873 16-17 16.897043724205552 32.424910725677215 31.027646648935647 19.650398901181585 18-19 16.509859415585325 30.387721739878177 35.94880226116493 17.15361658337158 20-21 18.37668510156642 28.507390005150057 35.843277492753934 17.272647400529586 22-23 17.69086757436038 28.974774065769317 35.629844535564835 17.70451382430547 24-25 17.74785561465837 29.606257483266923 36.02296317274628 16.622923729328424 26-27 17.495009938062942 28.879828653120242 37.14293191784285 16.482229490973957 28-29 16.438216603373615 30.126994245208156 36.55830360289038 16.876485548527842 30-31 17.258311986992638 29.30316290377532 36.317661590747534 17.120863518484505 32-33 17.04673653671262 29.883745166563696 36.248412221431906 16.82110607529178 34-35 17.688425159717195 29.540798751894666 36.00814745814917 16.76262863023898 36-37 17.873237382359704 28.880002692660817 36.13373174740598 17.1130281775735 38-39 16.874982763029163 30.3265511988284 35.721080336203904 17.077385701938532 40-41 16.959345861881843 30.218973052886046 35.97095939353686 16.850721691695252 42-43 16.766579015508924 30.28807099837255 35.06072795018747 17.884622035931056 44-45 17.30354186860138 29.90940862300546 34.712845337563174 18.074204170829987 46-47 18.156998054453798 30.71317844673162 33.61495383358593 17.514869665228655 48-49 17.73400350038439 30.557888366245827 33.674305355961984 18.0338027774078 50-51 16.938041790257973 31.160852442376115 33.73303155790242 18.16807420946349 52-53 17.515717729991994 32.10702403657197 32.50019511342516 17.87706312001088 54-55 17.34564549098927 31.123778029724527 32.637156495381305 18.893419983904902 56-57 17.93850962387757 31.383056778681194 32.11406582539716 18.56436777204407 58-59 18.218119226072275 30.88761279124057 32.44424524567424 18.450022737012915 60-61 17.73632755810709 31.46497309469648 32.806101869513135 17.992597477683297 62-63 17.25184653434806 31.285694523116497 32.75254663772781 18.709912304807627 64-65 17.094835152406795 31.822226501051066 32.29812677650587 18.784811570036275 66-67 17.331640869998438 31.24104422993697 31.569644461186286 19.8576704388783 68-69 17.08469956592127 31.053407387190003 31.997042581590723 19.864850465298005 70-71 17.727600288253665 31.13401271718334 31.95914350153427 19.17924349302873 72-73 17.838405968458467 30.123302280325753 32.66071029665639 19.37758145455939 74-75 17.024117410091307 31.8008096151851 31.329032583693106 19.84604039103049 76-77 17.080798141173926 30.802520337350142 31.91908992085189 20.197591600624044 78-79 17.213679008306947 30.703321610917033 31.92859062284586 20.154408757930156 80-81 17.454387233332568 30.49113963332191 31.554981850233936 20.49949128311158 82-83 17.099072751243007 30.707585064009184 31.896590803408404 20.296751381339405 84-85 16.89709512464354 30.630783883591878 31.44815686831649 21.02396412344809 86-87 16.90611146850693 30.598327387566528 31.497748406339554 20.997812737586994 88-89 17.291225053603632 30.17669841040415 31.04463379415173 21.487442741840493 90-91 17.133411625935278 29.65536397379841 31.55676690653355 21.65445749373276 92-93 17.633478550618708 30.073857991141157 31.355390408929505 20.93727304931063 94-95 17.350318864258135 29.50080896091284 31.367354244497804 21.78151793033122 96-97 17.030052594142767 29.40556561369336 31.40927440271953 22.155107389444346 98-99 17.24255412220451 29.623445080502737 30.502897313272964 22.631103484019796 100-101 17.176402851338487 28.74504560543274 31.48730679555297 22.5912447476758 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7998.0 1 4918.0 2 2219.5 3 2564.0 4 2136.0 5 1920.5 6 3127.5 7 5917.0 8 8648.5 9 8793.5 10 6349.5 11 4385.5 12 4133.0 13 4281.5 14 4341.5 15 4327.0 16 4300.0 17 4418.5 18 4694.5 19 5017.5 20 5322.5 21 5780.5 22 6456.5 23 7176.5 24 7868.0 25 9417.5 26 13127.5 27 17306.0 28 18659.5 29 19878.0 30 23244.5 31 26526.5 32 30582.5 33 35246.5 34 40169.5 35 43055.0 36 44764.0 37 47895.0 38 46086.0 39 41146.5 40 38295.0 41 36396.5 42 34549.0 43 33440.5 44 32392.0 45 30505.0 46 29343.5 47 32765.5 48 44358.5 49 41753.0 50 27317.5 51 20131.5 52 15362.0 53 12498.0 54 10523.5 55 8979.5 56 7505.0 57 6521.0 58 5677.5 59 4716.5 60 3915.5 61 3143.0 62 2381.5 63 1673.5 64 1100.5 65 825.0 66 646.0 67 513.0 68 431.0 69 348.0 70 282.5 71 237.0 72 210.0 73 194.0 74 166.5 75 152.5 76 120.5 77 81.0 78 59.0 79 45.0 80 50.0 81 38.5 82 18.0 83 10.0 84 7.0 85 4.0 86 3.0 87 2.0 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3449884147076665 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.346746537731413E-4 16-17 0.0013552782479710548 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.7386986150925655E-4 26-27 0.0013552782479710548 28-29 0.0 30-31 0.0033180950208946515 32-33 0.0 34-35 0.0102346874588159 36-37 0.029629186524608584 38-39 0.022665860353998677 40-41 0.013786451143153836 42-43 0.011216095845277697 44-45 0.0029909588920740524 46-47 0.0010748758518391126 48-49 7.944734557071702E-4 50-51 4.673373268865707E-5 52-53 4.673373268865707E-5 54-55 0.0 56-57 0.0 58-59 0.00462663953617705 60-61 0.011262829577966354 62-63 0.004579905803488393 64-65 0.0022432191690555394 66-67 0.004673373268865707 68-69 0.003645231149715251 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.21829326538871716 78-79 3.271361288205995E-4 80-81 0.003972367278535851 82-83 5.140710595752277E-4 84-85 0.0 86-87 0.11248809458159756 88-89 0.41420107281956764 90-91 0.39541411227872747 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0019160830402349398 100-101 7.010059903298561E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1069891.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.93046133455339 #Duplication Level Percentage of deduplicated Percentage of total 1 72.64384265393939 25.374829370168108 2 13.488685183600696 9.423319925194546 3 5.142401042783746 5.38879322375174 4 2.6108693583121503 3.6479548468037124 5 1.4431358701429313 2.520470085626737 6 0.8948915139338651 1.8755384055652098 7 0.5872511385280837 1.4359067231619385 8 0.4294755312294888 1.200142275019875 9 0.30744491028761045 0.9665273296175954 >10 1.9520571312088997 13.638807381472523 >50 0.27364668939251147 6.647661251665807 >100 0.20088715302461257 13.456350564151878 >500 0.017119544330863348 4.210902613921225 >1k 0.0069548148844132355 4.990910491548188 >5k 8.024786405092194E-4 2.429816106575131 >10k+ 5.349857603394796E-4 2.792069405755742 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17440 1.6300725961803586 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12442 1.1629222042245426 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9747 0.9110273850326809 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8148 0.7615729078943556 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8110 0.7580211442100176 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4518 0.4222860085747053 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4092 0.3824688683239695 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3443 0.32180848329409256 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3383 0.3162004353714537 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2654 0.24806265311139172 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2568 0.2400244510889427 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2260 0.21123647175272994 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2208 0.2063761635531096 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2099 0.19618820982698235 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2067 0.19319725093490833 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1992 0.18618719103160977 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1918 0.17927059859368852 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1757 0.16422233666794095 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1754 0.163941934271809 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1746 0.16319419454879047 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1717 0.16048363805284838 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1707 0.15954896339907523 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1411 0.13188259364739024 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1396 0.13048058166673054 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1387 0.1296393744783347 No Hit CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT 1387 0.1296393744783347 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1357 0.1268353505170153 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1304 0.12188157485201764 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1148 0.10730065025315663 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1081 0.10103833007287658 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 9.346746537731414E-5 0.0 16-17 0.0 0.0 0.0 3.7386986150925655E-4 0.0 18-19 0.0 0.0 0.0 3.7386986150925655E-4 0.0 20-21 0.0 0.0 0.0 7.01005990329856E-4 0.0 22-23 0.0 0.0 0.0 0.0019628167729235967 0.0 24-25 0.0 0.0 0.0 0.003645231149715251 0.0 26-27 0.0 0.0 0.0 0.0069633261706099035 0.0 28-29 0.0 0.0 0.0 0.018085954550510284 0.0 30-31 0.0 0.0 0.0 0.053977461255398915 0.0 32-33 0.0 0.0 0.0 0.1139835740276346 0.0 34-35 0.0 0.0 0.0 0.19880529885754716 0.0 36-37 0.0 0.0 0.0 0.32199541822484723 0.0 38-39 0.0 0.0 0.0 0.49598510502471743 0.0 40-41 0.0 0.0 0.0 0.6887617523654279 0.0 42-43 0.0 0.0 0.0 0.876678091506518 0.0 44-45 0.0 0.0 0.0 1.096092966479763 0.0 46-47 0.0 0.0 0.0 1.3572410647439785 0.0 48-49 0.0 0.0 0.0 1.6265208324960208 0.0 50-51 0.0 0.0 0.0 1.923420236267059 0.0 52-53 0.0 0.0 0.0 2.195317093049666 0.0 54-55 0.0 0.0 0.0 2.4961888640992402 0.0 56-57 0.0 0.0 0.0 2.8644974114185464 0.0 58-59 0.0 0.0 0.0 3.257154233468643 0.0 60-61 0.0 0.0 0.0 3.688319651254193 0.0 62-63 0.0 0.0 0.0 4.104109670985175 0.0 64-65 0.0 0.0 0.0 4.5399951957722795 0.0 66-67 0.0 0.0 0.0 5.032194868449216 0.0 68-69 0.0 0.0 0.0 5.587905683850037 0.0 70-71 0.0 0.0 0.0 6.221007560583274 0.0 72-73 0.0 0.0 0.0 6.838313435667746 0.0 74-75 0.0 0.0 0.0 7.466274601805231 0.0 76-77 0.0 0.0 0.0 8.140829299433307 0.0 78-79 0.0 0.0 0.0 8.929367571089017 0.0 80-81 0.0 0.0 0.0 9.752815941063155 0.0 82-83 0.0 0.0 0.0 10.551495432712304 0.0 84-85 0.0 0.0 0.0 11.375971944805592 0.0 86-87 0.0 0.0 0.0 12.248023396775935 0.0 88-89 0.0 0.0 0.0 13.160312592591207 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 65 2.0736479E-10 36.49958 94-95 CGTCACT 55 0.0025446757 34.641453 1 CATTCCG 295 0.0 30.560667 9 CATGGGG 2600 0.0 25.914701 4 GTACATG 7925 0.0 24.402006 1 TACATGG 8020 0.0 23.488073 2 AGTACTT 10620 0.0 22.965178 12-13 ACATGGG 7850 0.0 22.848272 3 TAGTCGA 105 0.0022097523 22.594978 5 ATGGGGG 1095 0.0 22.533075 5 GAGTACT 10535 0.0 22.362272 12-13 CATGGGT 765 0.0 21.088648 4 GTACTTT 11260 0.0 20.859219 14-15 GTAGTAC 160 5.0299495E-5 20.759136 3 GGATGAC 275 1.2751116E-9 20.705217 8 TAGACTA 165 6.3741856E-5 20.130072 5 CATATAG 455 0.0 19.814058 3 GTCGAAT 120 0.0047927117 19.770607 7 CCACGCG 60 0.00479425 19.770607 92-93 GTATCAA 21130 0.0 19.521679 1 >>END_MODULE