##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939074_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1055558 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.34672372337664 34.0 31.0 34.0 31.0 34.0 2 32.43116152783646 34.0 31.0 34.0 31.0 34.0 3 32.46401145176296 34.0 31.0 34.0 31.0 34.0 4 35.90831294917001 37.0 35.0 37.0 35.0 37.0 5 35.96887712470561 37.0 37.0 37.0 35.0 37.0 6 36.08779148090394 37.0 37.0 37.0 35.0 37.0 7 36.04831188811984 37.0 37.0 37.0 35.0 37.0 8 36.0571555518503 37.0 37.0 37.0 35.0 37.0 9 37.76037697596911 39.0 38.0 39.0 35.0 39.0 10-11 37.69884364478314 39.0 38.0 39.0 35.0 39.0 12-13 37.6421262498129 39.0 38.0 39.0 35.0 39.0 14-15 38.91195699336275 40.5 38.5 41.0 36.0 41.0 16-17 38.8734943982235 40.0 38.5 41.0 36.0 41.0 18-19 38.89343077310768 40.0 38.5 41.0 35.5 41.0 20-21 38.905827060189964 40.5 39.0 41.0 35.0 41.0 22-23 38.901400965176705 41.0 39.0 41.0 35.0 41.0 24-25 38.841658629843174 41.0 39.0 41.0 35.0 41.0 26-27 38.69970574804985 41.0 38.0 41.0 35.0 41.0 28-29 38.55363324421775 40.0 38.0 41.0 35.0 41.0 30-31 38.247066954160736 40.0 37.5 41.0 35.0 41.0 32-33 37.90544195581863 40.0 36.5 41.0 34.5 41.0 34-35 37.55638534310763 40.0 36.0 41.0 33.5 41.0 36-37 37.24996257903403 40.0 35.5 41.0 33.0 41.0 38-39 37.02779572510464 40.0 35.0 41.0 33.0 41.0 40-41 36.82640224412112 40.0 35.0 41.0 33.0 41.0 42-43 36.502729362100425 40.0 35.0 41.0 31.5 41.0 44-45 36.03621117930042 40.0 35.0 41.0 30.0 41.0 46-47 35.482216041183904 40.0 35.0 41.0 25.5 41.0 48-49 35.1147611026585 40.0 35.0 41.0 22.5 41.0 50-51 33.951676743485436 38.5 34.0 40.0 17.5 40.5 52-53 34.09078278976617 38.5 35.0 40.0 15.0 40.5 54-55 34.588057217130654 39.5 35.0 41.0 11.5 41.0 56-57 34.49206628153071 39.5 35.0 41.0 7.5 41.0 58-59 34.31811515804911 39.0 35.0 41.0 2.0 41.0 60-61 34.03429702583847 39.0 35.0 41.0 2.0 41.0 62-63 33.69394718243811 39.0 35.0 41.0 2.0 41.0 64-65 33.35521449318749 38.0 35.0 40.0 2.0 41.0 66-67 32.95374389659308 37.5 34.0 40.0 2.0 41.0 68-69 32.5156557005868 37.0 34.0 39.5 2.0 41.0 70-71 32.06372174717069 36.5 33.5 39.0 2.0 41.0 72-73 31.581909757682666 36.0 33.0 39.0 2.0 40.5 74-75 31.10734085668433 35.0 32.5 38.0 2.0 40.0 76-77 30.638458995147587 35.0 32.0 37.0 2.0 39.5 78-79 30.161827204189635 35.0 31.5 37.0 2.0 39.0 80-81 29.653246434587203 35.0 31.0 36.0 2.0 39.0 82-83 29.218606177964638 35.0 30.5 36.0 2.0 37.5 84-85 28.793840793210794 35.0 30.0 35.5 2.0 37.0 86-87 28.376799285306916 35.0 29.0 35.0 2.0 37.0 88-89 27.900116336572694 34.0 29.0 35.0 2.0 36.0 90-91 27.461967982810986 34.0 27.5 35.0 2.0 36.0 92-93 27.035336286589654 34.0 26.5 35.0 2.0 35.5 94-95 26.534339183635574 34.0 25.0 35.0 2.0 35.0 96-97 25.97809499809579 34.0 22.0 35.0 2.0 35.0 98-99 25.399092233681145 34.0 10.0 35.0 2.0 35.0 100-101 22.90851379081017 30.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14818096527663016 1101 2 0.23249529900571275 1101 3 0.2373128042856152 1101 4 0.28236989297109005 1101 5 0.14649009985963346 1101 6 0.25095574061030135 1101 7 0.33665665690735125 1101 8 0.2523192351324539 1101 9 0.3313944706311105 1101 10-11 0.29570972868842915 1101 12-13 0.3960202948643925 1101 14-15 0.4353276965775166 1101 16-17 0.3387573750293953 1101 18-19 0.39583401246222394 1101 20-21 0.39038540163752344 1101 22-23 0.38280012002800845 1101 24-25 0.47340491350453817 1101 26-27 0.41257707966978074 1101 28-29 0.3666640602228526 1101 30-31 0.5678616977885724 1101 32-33 0.6906056292200802 1101 34-35 0.7287047937184283 1101 36-37 0.682180517146108 1101 38-39 0.5204623154219021 1101 40-41 0.7462707500868362 1101 42-43 0.7216905305148984 1101 44-45 0.5106924345463071 1101 46-47 0.8658000525463265 1101 48-49 0.9108166179375914 1101 50-51 0.9342839212994463 1101 52-53 0.9963452819416361 1101 54-55 0.9196496517004462 1101 56-57 0.9024944222716869 1101 58-59 0.8777737217391248 1101 60-61 0.838476537816284 1101 62-63 0.7636263117599285 1101 64-65 0.6976363500257108 1101 66-67 0.7937172983345775 1101 68-69 0.800607046107352 1101 70-71 0.7775115098330545 1101 72-73 0.44007027532964216 1101 74-75 0.5227514256755512 1101 76-77 0.3292009867663772 1101 78-79 0.33794713299227297 1101 80-81 0.3489997803388718 1101 82-83 0.15411433610570668 1101 84-85 3.323560901051792E-4 1101 86-87 0.1351490145580314 1101 88-89 0.0337871296614729 1101 90-91 0.20923514251181174 1101 92-93 0.3406567566964469 1101 94-95 0.25244600597756417 1101 96-97 0.13273071784465174 1101 98-99 0.11747118052112882 1101 100-101 0.15037076115248027 1102 1 0.1423821302443855 1102 2 0.25168606247564895 1102 3 0.2516694716457124 1102 4 0.19348909413606208 1102 5 0.22400206158212654 1102 6 0.20640612546571901 1102 7 0.24183400244510977 1102 8 0.27624007973408027 1102 9 0.3420170002753764 1102 10-11 0.24732846178600454 1102 12-13 0.42402762792035276 1102 14-15 0.4361390134071286 1102 16-17 0.3621698464252745 1102 18-19 0.32717242519365897 1102 20-21 0.40179064399242037 1102 22-23 0.4198476548730241 1102 24-25 0.41364310780451774 1102 26-27 0.43091231994437607 1102 28-29 0.3073170142482269 1102 30-31 0.3711758750300973 1102 32-33 0.344238352332205 1102 34-35 0.4310191269824202 1102 36-37 0.43005211065557347 1102 38-39 0.4056609840301775 1102 40-41 0.3090840952085401 1102 42-43 0.07838389435929116 1102 44-45 0.06065602930394931 1102 46-47 0.30607205171421015 1102 48-49 0.5209957837374688 1102 50-51 0.4670993467207083 1102 52-53 0.5027916102129026 1102 54-55 0.4351218797012777 1102 56-57 0.4056824813521942 1102 58-59 0.3391763637195666 1102 60-61 0.41619002855895104 1102 62-63 0.290229442610773 1102 64-65 0.28964539932881195 1102 66-67 0.30888268214985004 1102 68-69 0.4953477887742892 1102 70-71 0.3715592527080318 1102 72-73 0.32156861108999024 1102 74-75 0.30639246998592995 1102 76-77 0.25717061439275213 1102 78-79 0.19749700817429883 1102 80-81 0.2857872777384962 1102 82-83 0.05598010477639548 1102 84-85 -0.10120966596107195 1102 86-87 -0.18599463012680317 1102 88-89 -0.1112968624333881 1102 90-91 -0.04553914498558598 1102 92-93 -0.1426423383732427 1102 94-95 -0.5210015704734445 1102 96-97 -0.49974665889760317 1102 98-99 -0.4740685011930381 1102 100-101 -0.5521672043145465 1103 1 0.1695647493873622 1103 2 0.09890272246764198 1103 3 0.2199764400077484 1103 4 0.25165889790057605 1103 5 0.17790674720487232 1103 6 0.15350264128470315 1103 7 0.13197717425127564 1103 8 0.27649538610172186 1103 9 0.2832715056818671 1103 10-11 0.297441096948198 1103 12-13 0.39876981854910554 1103 14-15 0.38031202101626604 1103 16-17 0.2617568508305581 1103 18-19 0.39319915727215715 1103 20-21 0.35114987506971573 1103 22-23 0.41996279303882744 1103 24-25 0.4074957309522915 1103 26-27 0.4644475622351294 1103 28-29 0.408025364268255 1103 30-31 0.5322817020224875 1103 32-33 0.41907315409432755 1103 34-35 0.36182609534445476 1103 36-37 0.13066034053142062 1103 38-39 0.05242710337338963 1103 40-41 0.01873317410321107 1103 42-43 -0.05157205277727428 1103 44-45 0.07400204452221004 1103 46-47 0.02327769849033956 1103 48-49 -0.007215569886881212 1103 50-51 -0.11457115788481786 1103 52-53 5.439129762194739E-4 1103 54-55 -0.05686850876487881 1103 56-57 0.07290314614994742 1103 58-59 -0.011329243008503909 1103 60-61 -0.08366229422839666 1103 62-63 -0.1389130283543878 1103 64-65 -0.2632831148882602 1103 66-67 -0.07453742197510849 1103 68-69 0.00869130098894999 1103 70-71 -0.10539809611055162 1103 72-73 -0.18943959876183314 1103 74-75 -0.30828013714262426 1103 76-77 -0.2168982682664371 1103 78-79 -0.15329143796106237 1103 80-81 -0.17492057168080777 1103 82-83 -0.08692388000534379 1103 84-85 -0.1726003347636329 1103 86-87 -0.1832463321692508 1103 88-89 -0.3077901543837278 1103 90-91 -0.2112580076207493 1103 92-93 -0.3317567756980324 1103 94-95 -0.456539215647652 1103 96-97 -0.5135106757177859 1103 98-99 -0.5480698027549131 1103 100-101 -0.7574735719557175 1104 1 0.0682479311101929 1104 2 0.08610164689123678 1104 3 0.2379789954552365 1104 4 0.20322935387631702 1104 5 0.18590682348688858 1104 6 0.1942849133445108 1104 7 0.27024309335420327 1104 8 0.15789592388992446 1104 9 0.3201014158597957 1104 10-11 0.3475178557253926 1104 12-13 0.29110013874286267 1104 14-15 0.46828187054998693 1104 16-17 0.35159084209626457 1104 18-19 0.33466701393825105 1104 20-21 0.4035222457240195 1104 22-23 0.41384116136652693 1104 24-25 0.5357210298824455 1104 26-27 0.5010963026283619 1104 28-29 0.38970337788459375 1104 30-31 0.5896553122595378 1104 32-33 0.4863920319858934 1104 34-35 0.47506674603003773 1104 36-37 0.4955553574088185 1104 38-39 0.5119380403072356 1104 40-41 0.5736133159877568 1104 42-43 0.6048990458744399 1104 44-45 0.471803215451132 1104 46-47 0.48989239803456286 1104 48-49 0.32042760191928465 1104 50-51 0.2380138488852097 1104 52-53 0.3303349002561973 1104 54-55 0.2192127887921842 1104 56-57 0.03079070646041515 1104 58-59 -0.021267359224147242 1104 60-61 0.001065569684492118 1104 62-63 0.025808446939770135 1104 64-65 0.015348862532270857 1104 66-67 0.12728095054811206 1104 68-69 0.17914107881758667 1104 70-71 0.13882115746994117 1104 72-73 0.0668608188821942 1104 74-75 0.11207428816775078 1104 76-77 0.19061217283431375 1104 78-79 0.25212363155092277 1104 80-81 0.09406649851771931 1104 82-83 0.07386430391059307 1104 84-85 0.029580377328972673 1104 86-87 -0.014620171252342828 1104 88-89 0.12601374362721884 1104 90-91 0.24818379873735807 1104 92-93 0.02280679582589329 1104 94-95 0.044528732556855743 1104 96-97 0.2460055056045647 1104 98-99 0.29013604426150863 1104 100-101 0.4015124493651072 1105 1 0.010511094303588209 1105 2 0.19407506881814385 1105 3 0.22526883739624282 1105 4 0.2246730264827832 1105 5 0.20307182902398324 1105 6 0.20397483582512876 1105 7 0.07732554578549156 1105 8 0.08382464633027098 1105 9 0.1613880362161808 1105 10-11 0.1494029860988988 1105 12-13 0.14047847358631316 1105 14-15 0.15474366456992783 1105 16-17 0.28139553352886537 1105 18-19 0.188575702149258 1105 20-21 0.16939106682540483 1105 22-23 0.09815103753687282 1105 24-25 0.1847319027305261 1105 26-27 0.1352199309380424 1105 28-29 0.1275469296816354 1105 30-31 0.1492546276728035 1105 32-33 0.30145294007005674 1105 34-35 0.11553286905430582 1105 36-37 0.24510232207206428 1105 38-39 0.4535468191253216 1105 40-41 0.43581506772439127 1105 42-43 0.40845789013096123 1105 44-45 0.237558777718327 1105 46-47 0.42475598406092985 1105 48-49 0.44146354272266564 1105 50-51 0.45810093234438654 1105 52-53 0.5725326250120588 1105 54-55 0.5839062348809776 1105 56-57 0.4349108111619202 1105 58-59 0.296285243212175 1105 60-61 0.3592529249648706 1105 62-63 0.23553863922810336 1105 64-65 0.3061094585254196 1105 66-67 0.25951693162130596 1105 68-69 0.1842880636157318 1105 70-71 0.0630761660272654 1105 72-73 0.06176417134368606 1105 74-75 -0.1085916737772834 1105 76-77 -0.16671944903218971 1105 78-79 -0.05016419689969709 1105 80-81 -0.04478883219807983 1105 82-83 -0.07294431382825906 1105 84-85 -0.06709232980885105 1105 86-87 -0.15108448213525705 1105 88-89 -0.06989887089004654 1105 90-91 -0.0073784684528170885 1105 92-93 -0.013083670297131533 1105 94-95 -0.11130283897238868 1105 96-97 -0.18752414304136167 1105 98-99 -0.10362716926914928 1105 100-101 -0.32087756372257914 1106 1 0.13515928063023352 1106 2 0.2624615189032795 1106 3 0.21045217445015396 1106 4 0.15438689199037725 1106 5 0.24886560383696832 1106 6 0.2190825794465212 1106 7 0.20676671466043217 1106 8 0.2555337932669204 1106 9 0.2848106196994351 1106 10-11 0.31780300535837114 1106 12-13 0.2723960167778756 1106 14-15 0.35815105933222213 1106 16-17 0.2689633246861476 1106 18-19 0.39689645116768446 1106 20-21 0.4394482762152734 1106 22-23 0.4613661001958178 1106 24-25 0.45316409406463265 1106 26-27 0.3771079721182886 1106 28-29 0.44193375620192654 1106 30-31 0.5482474447647121 1106 32-33 0.45580667531357477 1106 34-35 0.47341984220922484 1106 36-37 0.424362057954653 1106 38-39 0.3682339183422485 1106 40-41 0.42266281721116883 1106 42-43 0.42491598545659315 1106 44-45 0.4396180093528841 1106 46-47 0.4861280464441151 1106 48-49 0.49537584208491836 1106 50-51 0.7228858609311359 1106 52-53 0.7068171136949317 1106 54-55 0.7049553071434005 1106 56-57 0.8856043710566937 1106 58-59 0.9078216676909605 1106 60-61 1.1231011428070232 1106 62-63 1.0882966833410492 1106 64-65 0.9127938088902638 1106 66-67 0.9558617899985151 1106 68-69 0.8620650389154534 1106 70-71 0.8361908167961118 1106 72-73 0.894712315211958 1106 74-75 0.7890693945106833 1106 76-77 0.852738090395011 1106 78-79 0.9518460106211286 1106 80-81 1.0835263140644926 1106 82-83 0.9598515051151857 1106 84-85 0.9786204676734123 1106 86-87 0.9481598023971962 1106 88-89 1.0110128025393195 1106 90-91 1.0002071377172186 1106 92-93 0.8046155667651007 1106 94-95 0.7591203004092932 1106 96-97 0.8253862697679359 1106 98-99 0.6067650674122689 1106 100-101 0.3883842731934557 1107 1 0.0738900813428387 1107 2 0.16695860590795064 1107 3 0.15775344076052278 1107 4 0.14456456063113876 1107 5 0.14635705744571226 1107 6 0.18094882240477972 1107 7 0.15986989728838807 1107 8 0.16701243932595133 1107 9 0.24393238771907733 1107 10-11 0.2755404987379251 1107 12-13 0.1914941919427804 1107 14-15 0.2236838245110988 1107 16-17 0.1746761659399354 1107 18-19 0.31308163100302266 1107 20-21 0.32806833495666865 1107 22-23 0.2878743576949816 1107 24-25 0.26656387258069003 1107 26-27 0.1167439680738056 1107 28-29 0.16723348175443675 1107 30-31 0.06388372961232136 1107 32-33 0.01326009146264795 1107 34-35 -0.008030877613592224 1107 36-37 0.1386357350695846 1107 38-39 0.14830277095912692 1107 40-41 0.34446935580233884 1107 42-43 0.20565891963735083 1107 44-45 -0.014785187719674298 1107 46-47 0.12052305824054343 1107 48-49 0.026696560463271624 1107 50-51 -0.008348189817574792 1107 52-53 -0.06720907918869301 1107 54-55 -0.19902418151757928 1107 56-57 -0.20634757655754044 1107 58-59 -0.0783355859081567 1107 60-61 0.007267448358568629 1107 62-63 0.005577245717667267 1107 64-65 -0.1008443056073105 1107 66-67 0.01342354068463436 1107 68-69 -0.0621730081739571 1107 70-71 -0.12986436159475545 1107 72-73 -0.07347344240757181 1107 74-75 -0.09420616040289076 1107 76-77 0.05839315322904426 1107 78-79 0.03477008014782612 1107 80-81 -0.008365447695826589 1107 82-83 -0.08976571285390378 1107 84-85 -0.2914695703640504 1107 86-87 -0.33708503365653897 1107 88-89 -0.24553541836683834 1107 90-91 -0.25439106152203195 1107 92-93 -0.29042993374046233 1107 94-95 -0.14423325537095621 1107 96-97 -0.07669134590927484 1107 98-99 -0.1717630273443369 1107 100-101 0.10641354759279764 1108 1 0.03219266217821115 1108 2 0.04126976644291602 1108 3 0.06441853764820138 1108 4 0.16791535280889036 1108 5 0.14896203311744216 1108 6 0.1928765044018519 1108 7 0.1563398732865977 1108 8 0.2629928817266034 1108 9 0.1806244544650255 1108 10-11 0.22979710615533122 1108 12-13 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66-67 -1.1511966519638221 2316 68-69 -0.9682471195362794 2316 70-71 -0.9084332690763368 2316 72-73 -1.33359298339564 2316 74-75 -1.0317838208191148 2316 76-77 -1.0328215822518025 2316 78-79 -0.7453100863763567 2316 80-81 -0.8002073249406543 2316 82-83 -0.7918390199472398 2316 84-85 -0.7279768008290652 2316 86-87 -0.8575497738385955 2316 88-89 -0.8603628983288232 2316 90-91 -1.208089541656154 2316 92-93 -1.2785467147550875 2316 94-95 -1.1872471810860077 2316 96-97 -1.259730889616808 2316 98-99 -1.458921050289149 2316 100-101 -1.444324832094491 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4524.0 3 700.0 4 633.0 5 648.0 6 659.0 7 807.0 8 956.0 9 1155.0 10 1564.0 11 2417.0 12 4351.0 13 8151.0 14 7611.0 15 9131.0 16 13183.0 17 22319.0 18 17231.0 19 8518.0 20 7421.0 21 6766.0 22 6804.0 23 7404.0 24 8004.0 25 8684.0 26 9954.0 27 11132.0 28 12587.0 29 14756.0 30 17420.0 31 21227.0 32 25885.0 33 33275.0 34 46841.0 35 80907.0 36 109010.0 37 194287.0 38 266828.0 39 61792.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.637938215699215 22.673579347162498 15.823312038676523 18.865170398461764 2 14.256828528072838 23.999810318664643 41.30320561456753 20.44015553869499 3 18.469956031366063 29.872210230010204 32.69631293224126 18.96152080638247 4 10.832531836953821 19.793356211549515 41.5868490094643 27.787262942032363 5 10.14796286652799 40.9913768163369 35.403428595277596 13.457231721857507 6 24.465515410751124 41.37970589127533 19.151100442907413 15.003678255066133 7 22.843907267438553 38.10588851006701 23.341569232752963 15.708634989741471 8 23.672788708038166 34.59291521927097 21.77568906148082 19.95860701121005 9 25.61309167719812 18.57131886645234 24.500818656455344 31.314770799894198 10-11 22.153436013340944 29.26247490040747 28.85201716347585 19.732071922775734 12-13 24.682507402235036 28.712139096600236 29.075021640219617 17.530331860945118 14-15 21.52614671701934 29.059944929331984 30.572610756366092 18.841297597282587 16-17 17.009552061365273 32.26265490517857 31.10295473495649 19.624838298499668 18-19 16.61077357181054 30.402288061348713 36.03137219207124 16.95556617476951 20-21 18.536838352693998 28.48150592197331 35.7313722366969 17.250283488635805 22-23 17.824613705530087 28.878388892128655 35.54487539629823 17.75212200604303 24-25 17.81879255095683 29.550219968116714 35.8605879156468 16.770399565279654 26-27 17.565366489055755 28.86434127089876 36.954939430997044 16.615352809048435 28-29 16.509707269380634 30.175890619325017 36.407167628084466 16.90723448320988 30-31 17.249102285840532 29.41183721576336 36.07962276567076 17.259437732725345 32-33 17.02141680707806 29.84149078499847 36.104012842309075 17.033079565614393 34-35 17.749394867027586 29.591513262547153 35.77587005835796 16.8832218120673 36-37 17.94076361059897 29.021421551448295 35.82748289628061 17.210331941672127 38-39 16.971117660696663 30.478915830420217 35.46863998453744 17.081326524345684 40-41 17.086403865300813 30.354956985583176 35.66308260785968 16.895556541256337 42-43 16.863281090072256 30.448528438633925 34.73205691059233 17.95613356070149 44-45 17.431418050652898 30.071979028097434 34.52679430985739 17.96980861139228 46-47 18.128464250788667 30.862766868122083 33.538423010671394 17.47034587041786 48-49 17.744694770452316 30.670318949414803 33.5792735969573 18.00571268317558 50-51 16.938273438909782 31.20728246104446 33.6579092587223 18.196534841323462 52-53 17.53143366458823 32.05676723661808 32.525939200950724 17.88585989784297 54-55 17.380834491820764 31.11005313172478 32.68005751175944 18.829054864695017 56-57 17.90374598291119 31.353520949784485 32.28597103126856 18.456762036035762 58-59 18.352805345082693 30.704718827767287 32.505528928180325 18.436946898969694 60-61 17.851536709597752 31.204632675699383 33.02541426372459 17.91841635097827 62-63 17.430124888849114 30.95016632171017 33.08640541369074 18.53330337574997 64-65 17.264932602529434 31.498419400872223 32.720092298037336 18.51655569856101 66-67 17.504098944203406 30.883681416198915 31.990336825352834 19.62188281424485 68-69 17.282772856495797 30.682302578906285 32.490780435042495 19.544144129555427 70-71 17.927623510809802 30.863474328658892 32.32529176578726 18.88361039474405 72-73 18.093546967766265 29.911915795729215 32.89776937157268 19.09676786493184 74-75 17.274846365755455 31.564676385922557 31.87054008300178 19.289937165320207 76-77 17.51206286257025 30.60360731365347 32.07785932095665 19.806470502819625 78-79 17.563671151768418 30.563533124229547 32.18002598725387 19.69276973674817 80-81 17.87476497608111 30.264369183901753 31.83321036723231 20.027655472784826 82-83 17.61532232457015 30.347110711431046 32.29724898348971 19.74031798050909 84-85 17.466743149291066 30.19000854479113 31.953377334644014 20.389870971273794 86-87 17.447295085635073 30.321404055300764 32.006780732513334 20.224520126550825 88-89 17.982382013142793 29.738672399994858 31.582690896833892 20.696254690028457 90-91 17.95260915360836 29.17286487866107 32.09434170703044 20.78018426070013 92-93 18.584055625161614 29.47998321835238 32.02056466148836 19.91539649499765 94-95 18.43280295039892 28.73296175576354 32.11653980490987 20.71769548892767 96-97 18.09792564789872 28.58940152315664 32.11257625664232 21.20009657230232 98-99 18.352806497573315 28.802457542533656 31.266571554830897 21.578164405062132 100-101 18.683156018999224 27.79300426039999 32.19794078286814 21.325898937732653 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9007.0 1 5428.0 2 2326.5 3 2670.0 4 2148.0 5 2010.0 6 3176.5 7 5901.5 8 8954.0 9 9147.5 10 6565.0 11 4606.0 12 4394.5 13 4546.5 14 4555.0 15 4623.0 16 4650.5 17 4727.5 18 4808.5 19 4995.0 20 5343.5 21 5834.5 22 6393.0 23 7031.0 24 7849.0 25 9454.5 26 12955.5 27 17119.5 28 18258.0 29 19354.5 30 22827.0 31 25912.5 32 29902.5 33 34584.5 34 39799.0 35 42716.5 36 44045.0 37 47068.0 38 44976.0 39 40041.5 40 37191.0 41 35257.0 42 33554.5 43 32294.0 44 31237.0 45 29328.5 46 28410.0 47 31421.5 48 42699.5 49 41078.5 50 26738.5 51 19120.5 52 14570.5 53 12255.0 54 10809.5 55 9340.0 56 7632.0 57 6615.5 58 5861.0 59 4867.0 60 4106.5 61 3385.0 62 2635.5 63 1850.5 64 1252.0 65 968.0 66 780.5 67 660.5 68 560.5 69 457.5 70 400.0 71 342.0 72 291.5 73 259.5 74 213.0 75 195.0 76 160.5 77 116.5 78 100.5 79 99.5 80 79.5 81 51.0 82 33.5 83 20.0 84 16.5 85 15.0 86 9.0 87 6.5 88 5.0 89 2.0 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3755359724430112 2 0.10970500910418945 3 0.11084184857677172 4 0.12126287707544256 5 0.12382076588875268 6 0.06726300212778455 7 0.07825245036274653 8 0.07484193194499972 9 0.073989302340563 10-11 0.07199983326354402 12-13 0.0766419277765883 14-15 0.06929983951616113 16-17 0.07195246495218643 18-19 0.08204191527135411 20-21 0.07976823632618955 22-23 0.09042610638164839 24-25 0.10297870889141099 26-27 0.10416291667535087 28-29 0.09843135100108188 30-31 0.08857874223870218 32-33 0.08706295627525916 34-35 0.3331413337779639 36-37 0.5204830051972511 38-39 0.500161999624843 40-41 0.4717883811216437 42-43 0.5001146313134853 44-45 0.5107251330575866 46-47 0.508451454112422 48-49 0.5152251226365581 50-51 0.5133303901822543 52-53 0.5125251288891752 54-55 0.5057040920536816 56-57 0.4780409982208462 58-59 0.46870944088340005 60-61 0.41835692591027684 62-63 0.4911146521555424 64-65 0.5192987974133112 66-67 0.5298145625346973 68-69 0.5449724221691276 70-71 0.5400461177879378 72-73 0.5248408898421498 74-75 0.528677723062115 76-77 0.5049935673833176 78-79 0.4777567883527007 80-81 0.48628308439706774 82-83 0.49959357988855185 84-85 0.49362517265749484 86-87 0.49537780017772587 88-89 0.5044251476470265 90-91 0.5233251038787068 92-93 0.5306671921391339 94-95 0.5239408919263555 96-97 0.5274461469668176 98-99 0.5273040420327448 100-101 0.5025304151927227 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1055558.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.042072449924014 #Duplication Level Percentage of deduplicated Percentage of total 1 73.58225339856966 26.520569080199152 2 13.153295044576915 9.481440259037353 3 4.997829915377105 5.403964437072576 4 2.424856394402556 3.495873993908738 5 1.3470961772748213 2.4276069009177395 6 0.8566907440011327 1.8526145917480876 7 0.5836239710006564 1.4724512212422607 8 0.410592793967178 1.1838892182065404 9 0.2907152248933453 0.9430181278111716 >10 1.8726256181728882 13.442399162667018 >50 0.2682773132016684 6.688505429624418 >100 0.1876349118629803 12.905741628932269 >500 0.015811930773668065 3.7910348895110095 >1k 0.007378901027711764 5.175879142989087 >5k 7.905965386834033E-4 2.3882389512656235 >10k+ 5.270643591222689E-4 2.826772964867015 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17722 1.6789224277585884 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12039 1.1405342008681663 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9207 0.8722400853387497 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8293 0.7856508121770664 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7644 0.7241667440349085 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4595 0.4353147813762958 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4080 0.3865254206779732 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3456 0.32740976810369493 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3246 0.30751507733350514 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2573 0.24375733024618257 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2562 0.2427152273963155 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2432 0.23039946644334086 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2128 0.20159953313792328 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2092 0.19818901472017642 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1970 0.1866311467489233 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1844 0.17469433228680945 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1837 0.17403117592780312 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1807 0.17118907724634744 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1780 0.16863118843303732 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1706 0.1616206783521133 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1691 0.16019962901138546 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1688 0.15991541914323987 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1415 0.13405232114199314 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1360 0.12884180689265773 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1351 0.127989177288221 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1226 0.11614709944882233 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1178 0.11159974155849324 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1167 0.11055763870862616 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1128 0.10686291042273377 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1062 0.10061029332353125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 9.473662271518951E-5 0.0 18-19 0.0 0.0 0.0 1.4210493407278426E-4 0.0 20-21 0.0 0.0 0.0 5.210514249335423E-4 0.0 22-23 0.0 0.0 0.0 0.001326312718012653 0.0 24-25 0.0 0.0 0.0 0.0038368332199651745 0.0 26-27 0.0 0.0 0.0 0.009094715780658192 0.0 28-29 0.0 0.0 0.0 0.022784157763003077 0.0 30-31 0.0 0.0 0.0 0.05641565882689535 0.0 32-33 0.0 0.0 0.0 0.11695236074190143 0.0 34-35 0.0 0.0 0.0 0.20723636218947702 0.0 36-37 0.0 0.0 0.0 0.3300150252283626 0.0 38-39 0.0 0.0 0.0 0.5020567320791468 0.0 40-41 0.0 0.0 0.0 0.694656286059127 0.0 42-43 0.0 0.0 0.0 0.880861118005832 0.0 44-45 0.0 0.0 0.0 1.1007921876391444 0.0 46-47 0.0 0.0 0.0 1.3634494741170073 0.0 48-49 0.0 0.0 0.0 1.6339225319688735 0.0 50-51 0.0 0.0 0.0 1.932390261833078 0.0 52-53 0.0 0.0 0.0 2.2118159305315293 0.0 54-55 0.0 0.0 0.0 2.506257353930338 0.0 56-57 0.0 0.0 0.0 2.871561771120109 0.0 58-59 0.0 0.0 0.0 3.2590819263365916 0.0 60-61 0.0 0.0 0.0 3.6987072240464283 0.0 62-63 0.0 0.0 0.0 4.129143069352892 0.0 64-65 0.0 0.0 0.0 4.57701045323895 0.0 66-67 0.0 0.0 0.0 5.076130350013926 0.0 68-69 0.0 0.0 0.0 5.637539576224139 0.0 70-71 0.0 0.0 0.0 6.262943391078462 0.0 72-73 0.0 0.0 0.0 6.872005138514416 0.0 74-75 0.0 0.0 0.0 7.520572057622603 0.0 76-77 0.0 0.0 0.0 8.192254712673297 0.0 78-79 0.0 0.0 0.0 8.958816095373251 0.0 80-81 0.0 0.0 0.0 9.787856280753875 0.0 82-83 0.0 0.0 0.0 10.600791240272917 0.0 84-85 0.0 0.0 0.0 11.418320925993644 0.0 86-87 0.0 4.7368311357594754E-5 0.0 12.27076105718492 0.0 88-89 0.0 9.473662271518951E-5 0.0 13.19638522942368 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACG 55 0.0025827005 34.536793 1 ATGGGCG 160 5.4569682E-11 32.569088 5 AGACCCG 60 0.0040028305 31.582148 5 CATATAG 290 0.0 29.405464 3 GTGTACG 85 6.416215E-4 27.934172 1 GTACATG 8110 0.0 25.120087 1 TACATGG 8125 0.0 24.89763 2 CATGGGT 785 0.0 24.743876 4 GAGTACT 10340 0.0 24.434454 12-13 GGTCGGG 175 1.4894977E-7 24.422445 1 GTACGTA 100 0.0016790131 23.687735 3 AGTACTT 10425 0.0 23.531113 12-13 CATGGGG 2560 0.0 23.502674 4 ACATGGG 8165 0.0 23.26707 3 CATTCCG 285 3.6379788E-12 23.262228 9 GTACTTT 11080 0.0 22.611288 14-15 GTATAGG 365 0.0 22.117739 1 GACATAT 365 0.0 22.117739 1 GTATCAA 20780 0.0 22.007233 1 GGATATG 540 0.0 21.985226 1 >>END_MODULE