##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939074_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1055558 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.38372026927938 34.0 31.0 34.0 31.0 34.0 2 32.58579253816465 34.0 31.0 34.0 31.0 34.0 3 32.66239941339083 34.0 31.0 34.0 31.0 34.0 4 36.16396351503186 37.0 35.0 37.0 35.0 37.0 5 36.14325408930632 37.0 35.0 37.0 35.0 37.0 6 36.214170135606004 37.0 37.0 37.0 35.0 37.0 7 36.16761182237262 37.0 37.0 37.0 35.0 37.0 8 36.16433109312799 37.0 37.0 37.0 35.0 37.0 9 37.85365654942694 39.0 38.0 39.0 35.0 39.0 10-11 37.788415226827894 39.0 38.0 39.0 35.0 39.0 12-13 37.71905949270433 39.0 38.0 39.0 35.0 39.0 14-15 38.99626548233257 40.0 38.0 41.0 36.0 41.0 16-17 39.10493359910114 40.0 38.5 41.0 36.0 41.0 18-19 39.213875978392466 41.0 39.0 41.0 36.0 41.0 20-21 39.21453392423723 41.0 39.0 41.0 35.5 41.0 22-23 39.1134561056806 41.0 39.0 41.0 35.0 41.0 24-25 39.01627575178247 41.0 38.5 41.0 35.0 41.0 26-27 38.81670689815245 40.0 38.0 41.0 35.0 41.0 28-29 38.630888591626416 40.0 38.0 41.0 35.0 41.0 30-31 38.31174222543906 40.0 37.5 41.0 34.0 41.0 32-33 37.973070641310095 40.0 36.5 41.0 34.0 41.0 34-35 37.745265537279806 40.0 36.0 41.0 33.0 41.0 36-37 37.49949694853338 40.0 35.5 41.0 33.0 41.0 38-39 37.29503494834012 40.0 35.0 41.0 33.0 41.0 40-41 37.05367540201486 40.0 35.0 41.0 33.0 41.0 42-43 36.72261543183795 40.0 35.0 41.0 31.5 41.0 44-45 36.24676142855248 40.0 35.0 41.0 30.5 41.0 46-47 35.60874532711608 40.0 35.0 41.0 27.5 41.0 48-49 35.002843993413904 39.0 35.0 41.0 23.5 41.0 50-51 34.66683735048193 39.0 35.0 41.0 21.5 41.0 52-53 34.414722829062924 39.0 35.0 40.5 19.5 41.0 54-55 34.18276636622525 39.0 35.0 40.0 16.5 41.0 56-57 33.96231803463192 38.5 34.5 40.0 11.5 41.0 58-59 33.64800323620303 38.0 34.0 40.0 7.5 41.0 60-61 33.268554167558776 38.0 34.0 40.0 2.0 41.0 62-63 32.88161853730443 37.5 33.0 40.0 2.0 41.0 64-65 32.45483715721922 37.0 33.0 40.0 2.0 41.0 66-67 32.01205949838853 36.0 32.5 39.0 2.0 40.5 68-69 31.4952825898719 36.0 31.5 39.0 2.0 40.0 70-71 30.998483740353443 35.0 31.0 38.5 2.0 40.0 72-73 30.412461939561823 35.0 30.5 37.5 2.0 39.5 74-75 29.865763416126825 35.0 30.0 37.0 2.0 39.0 76-77 27.883117270675793 32.5 27.0 35.0 2.0 37.5 78-79 28.793203689423034 34.0 29.0 36.0 2.0 38.0 80-81 28.741614387840364 34.0 29.0 36.0 2.0 37.5 82-83 28.32125757182457 34.0 29.0 35.0 2.0 37.0 84-85 27.83826421665129 34.0 27.5 35.0 2.0 36.5 86-87 27.16264525492678 33.5 26.0 35.0 2.0 36.0 88-89 26.46297076996243 33.0 25.0 35.0 2.0 35.5 90-91 25.90555090293475 32.5 23.5 35.0 2.0 35.0 92-93 25.47185801253934 32.0 20.5 34.5 2.0 35.0 94-95 24.94873801344881 32.0 18.5 34.0 2.0 35.0 96-97 24.18771019688165 31.5 4.5 34.0 2.0 35.0 98-99 23.09472099117244 31.0 2.0 34.0 2.0 35.0 100-101 17.685103518707642 23.5 2.0 30.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20382054764548485 1101 2 0.2629532691622458 1101 3 0.23608588207735437 1101 4 0.1619100245142917 1101 5 0.16847256564905422 1101 6 0.2066270829912753 1101 7 0.28523358803872156 1101 8 0.217734022112829 1101 9 0.352160315899944 1101 10-11 0.25226622699116064 1101 12-13 0.19301558204831792 1101 14-15 0.2829268200964137 1101 16-17 0.35997638638708196 1101 18-19 0.4025996084081882 1101 20-21 0.3265180727832018 1101 22-23 0.3296067926311892 1101 24-25 0.352427544370876 1101 26-27 0.3188582830236939 1101 28-29 0.3105719628667529 1101 30-31 0.31060996250776185 1101 32-33 0.5890721062918871 1101 34-35 0.575451190515814 1101 36-37 0.7921155241864213 1101 38-39 0.7358785695908807 1101 40-41 0.7281077406182206 1101 42-43 0.8789370106850569 1101 44-45 0.9561466679835888 1101 46-47 0.8220163084736143 1101 48-49 0.984923961822453 1101 50-51 1.1019483293900478 1101 52-53 1.0467720358208794 1101 54-55 1.1154422772921748 1101 56-57 1.0963070267893755 1101 58-59 1.0598728308160332 1101 60-61 0.9352569898387131 1101 62-63 1.0243543170399718 1101 64-65 0.9793861309873364 1101 66-67 1.0220975477455738 1101 68-69 1.1065128419904902 1101 70-71 1.1717650422044947 1101 72-73 1.18098560413436 1101 74-75 1.0914894625070488 1101 76-77 0.8883772327014618 1101 78-79 1.1730056101245019 1101 80-81 1.371101833763781 1101 82-83 1.239419479259805 1101 84-85 1.3040580137830737 1101 86-87 1.4878124089952607 1101 88-89 1.5289379245678099 1101 90-91 1.6725884507258257 1101 92-93 1.6083692170699244 1101 94-95 1.3044287464982567 1101 96-97 1.0829589531260346 1101 98-99 0.9922472026926208 1101 100-101 -0.06169329405943458 1102 1 0.21784436707548593 1102 2 0.21834124503076424 1102 3 0.15595794365422222 1102 4 0.15844892611612238 1102 5 0.20261288185458426 1102 6 0.2513275198541862 1102 7 0.20141571826547278 1102 8 0.23888286754657173 1102 9 0.29491827304577356 1102 10-11 0.3125663102031382 1102 12-13 0.30708880859293686 1102 14-15 0.41316787480829475 1102 16-17 0.2503211960396712 1102 18-19 0.3982036753938374 1102 20-21 0.4141064855146368 1102 22-23 0.21389272980614038 1102 24-25 0.30372123066170076 1102 26-27 0.43023492125960416 1102 28-29 0.43579819544424225 1102 30-31 0.2846036175940938 1102 32-33 0.2686279623351595 1102 34-35 0.21399654104629207 1102 36-37 0.27459030055421607 1102 38-39 0.3598657237410805 1102 40-41 0.4525772642296175 1102 42-43 0.4268918161770543 1102 44-45 0.37051113646705147 1102 46-47 0.5682223621453417 1102 48-49 0.42452896494290115 1102 50-51 0.2828862844555715 1102 52-53 0.535412300018919 1102 54-55 0.5865573178580235 1102 56-57 0.6485706527515163 1102 58-59 0.33616911748131173 1102 60-61 0.30014673396186 1102 62-63 0.33211513459769293 1102 64-65 0.47604725018850047 1102 66-67 0.5550624946979354 1102 68-69 0.672587056677397 1102 70-71 0.5253691611976237 1102 72-73 0.767502662590779 1102 74-75 0.5546824622990201 1102 76-77 0.4306203157054149 1102 78-79 0.4996048404136637 1102 80-81 0.7120044237367367 1102 82-83 0.671045233368396 1102 84-85 0.6958395303214573 1102 86-87 0.6130464426961133 1102 88-89 0.34580785383762347 1102 90-91 0.7063426113249491 1102 92-93 0.9135270597992822 1102 94-95 0.9848791313536758 1102 96-97 0.7445696250627911 1102 98-99 0.9596515089127173 1102 100-101 0.7250838018423771 1103 1 -0.03927918118242246 1103 2 0.03299883413769322 1103 3 0.09595594125134 1103 4 0.15244672347294852 1103 5 0.09679089547092445 1103 6 0.1327352090812539 1103 7 0.0047947731313158215 1103 8 0.03101843022177775 1103 9 0.12553000712667028 1103 10-11 0.0595677118851583 1103 12-13 0.004335504628180331 1103 14-15 0.0046251235067344965 1103 16-17 0.0962312881502001 1103 18-19 0.1785676121179094 1103 20-21 0.32553519997198777 1103 22-23 0.27506113188864134 1103 24-25 0.2727115190077214 1103 26-27 0.22017785277741808 1103 28-29 0.20168225631729086 1103 30-31 0.1553034574018568 1103 32-33 0.2121101409494912 1103 34-35 0.2227119995965552 1103 36-37 0.2377185544589011 1103 38-39 0.26592549546715816 1103 40-41 0.28301378805820576 1103 42-43 0.21590613335767017 1103 44-45 0.20812627463285338 1103 46-47 0.358265413807338 1103 48-49 0.1916019525280035 1103 50-51 0.2791993601464 1103 52-53 0.23291680542541116 1103 54-55 0.10732223575949718 1103 56-57 0.1564626231159565 1103 58-59 0.04643393526264816 1103 60-61 0.18534897665309558 1103 62-63 0.20812885105744527 1103 64-65 0.22316379003632392 1103 66-67 0.22553205817411026 1103 68-69 0.05492336024167699 1103 70-71 -0.07496123527814547 1103 72-73 -0.0507342216703357 1103 74-75 -0.1600276898835986 1103 76-77 -0.12852565946479189 1103 78-79 -0.34519391809655176 1103 80-81 -0.26513063833775163 1103 82-83 -0.4150881266636439 1103 84-85 -0.4150860804114238 1103 86-87 -0.21850141473279905 1103 88-89 -0.2319379411163247 1103 90-91 0.0316054267034076 1103 92-93 -0.2004922633885471 1103 94-95 -0.04687897837800037 1103 96-97 -0.26498684269851935 1103 98-99 -0.2210903813556051 1103 100-101 -0.20149309042834673 1104 1 -0.0012573645262463629 1104 2 0.10080877749830108 1104 3 0.1257631384594191 1104 4 0.0709489261161238 1104 5 0.11078387752557717 1104 6 0.011608905135567227 1104 7 0.09692437627413142 1104 8 0.06848243464613546 1104 9 0.1709464115739152 1104 10-11 0.16824812838495262 1104 12-13 0.11910179560592837 1104 14-15 0.1041040219944378 1104 16-17 0.2793796375981117 1104 18-19 0.3341614676016249 1104 20-21 0.2994419833501354 1104 22-23 0.3040983575117622 1104 24-25 0.2856746938651682 1104 26-27 0.3948994234240999 1104 28-29 0.256631528777568 1104 30-31 0.25175729724777085 1104 32-33 0.2686279623351595 1104 34-35 0.4235744631242184 1104 36-37 0.5172039369178592 1104 38-39 0.48559624322160033 1104 40-41 0.5469495586019164 1104 42-43 0.6585206040558305 1104 44-45 0.6706193615752767 1104 46-47 0.509077340500319 1104 48-49 0.5782897874537269 1104 50-51 0.6178700506893406 1104 52-53 0.4012402220968454 1104 54-55 0.41144909274979113 1104 56-57 0.42938775231861115 1104 58-59 0.26135851142070265 1104 60-61 0.4075060413211702 1104 62-63 0.6144473856799451 1104 64-65 0.4496403237815798 1104 66-67 0.5079304600659071 1104 68-69 0.5333283986687398 1104 70-71 0.36270682353528727 1104 72-73 0.1596563422444568 1104 74-75 0.2786272674938246 1104 76-77 0.17439737198246874 1104 78-79 0.4313689096777331 1104 80-81 0.36581935880167293 1104 82-83 0.4904175277406928 1104 84-85 0.4282258939578192 1104 86-87 0.528630858280529 1104 88-89 0.8165059057856752 1104 90-91 1.033912957645299 1104 92-93 0.8561948087170279 1104 94-95 0.766156187630731 1104 96-97 0.4958142138073818 1104 98-99 0.22453246129367344 1104 100-101 -0.7337365444779671 1105 1 -0.06669580205770842 1105 2 0.08736872917452843 1105 3 0.12387549122293251 1105 4 -0.0035753867802839068 1105 5 -0.018079507151760765 1105 6 0.049160500826701536 1105 7 0.0525520819018368 1105 8 0.044723248096254054 1105 9 0.2311497741028532 1105 10-11 0.20709590851181048 1105 12-13 0.1392241151468525 1105 14-15 0.0924886993326055 1105 16-17 0.1533603080904058 1105 18-19 0.13894099040441432 1105 20-21 0.195369699045294 1105 22-23 0.07596989682516408 1105 24-25 0.07828518415006869 1105 26-27 0.1551556400756695 1105 28-29 0.25865760347806344 1105 30-31 0.3886998120972649 1105 32-33 0.24616496022099454 1105 34-35 0.2144986552111945 1105 36-37 0.3797832181017853 1105 38-39 0.37203538547469606 1105 40-41 0.4859282156038276 1105 42-43 0.49405619249839816 1105 44-45 0.39609253181588855 1105 46-47 0.6565151739846655 1105 48-49 0.7166669142029463 1105 50-51 0.5350400899523962 1105 52-53 0.6218684310971483 1105 54-55 0.4367687343485045 1105 56-57 0.28542318974940173 1105 58-59 0.18480812382380662 1105 60-61 0.1056964168371266 1105 62-63 0.11732919379431195 1105 64-65 0.17995845526250065 1105 66-67 0.21301704015248646 1105 68-69 0.2589903336330046 1105 70-71 0.27436493286781527 1105 72-73 0.30320562242164684 1105 74-75 0.2620159718127617 1105 76-77 0.33561503029315176 1105 78-79 0.20829934358490831 1105 80-81 0.6474563264851518 1105 82-83 0.5115843454191378 1105 84-85 0.524684667742175 1105 86-87 0.3604037365650363 1105 88-89 0.5576339637741974 1105 90-91 0.7301401800353133 1105 92-93 0.8097083494398696 1105 94-95 0.6337691947786226 1105 96-97 0.46679478362516136 1105 98-99 0.48198237572033165 1105 100-101 -0.31183480280878584 1106 1 0.10847348433503612 1106 2 0.1422166449931268 1106 3 0.1082118491690025 1106 4 0.03109385365235795 1106 5 0.027685816166652444 1106 6 -0.007495179185909251 1106 7 0.13894395090240153 1106 8 0.1063141681300408 1106 9 0.14920728113912674 1106 10-11 0.04607139207778488 1106 12-13 0.12872441935898848 1106 14-15 0.12356101427752009 1106 16-17 0.03032072549572007 1106 18-19 -0.06615379684406975 1106 20-21 0.157290656416194 1106 22-23 0.0849189861584776 1106 24-25 0.1487934591578437 1106 26-27 0.2120695721159862 1106 28-29 0.3587630928656651 1106 30-31 0.2830955242381705 1106 32-33 0.384593518518102 1106 34-35 0.33435933043082144 1106 36-37 0.4789957682749062 1106 38-39 0.5562719443320816 1106 40-41 0.667385282298504 1106 42-43 0.7466770128644171 1106 44-45 0.739436414087983 1106 46-47 0.7842043873664934 1106 48-49 0.7558730737326655 1106 50-51 0.8641527534937765 1106 52-53 1.0514849049502288 1106 54-55 1.1191471917522406 1106 56-57 0.9790301389175227 1106 58-59 0.9865373181212576 1106 60-61 1.1123573494333456 1106 62-63 1.3919036250927022 1106 64-65 1.2831618746944287 1106 66-67 1.4201034320214916 1106 68-69 1.0950167103570543 1106 70-71 1.1610316870775428 1106 72-73 1.2583529355062701 1106 74-75 1.0337091421409141 1106 76-77 0.9629113941921439 1106 78-79 1.1860272947708985 1106 80-81 1.3965458786586282 1106 82-83 1.2981532702590428 1106 84-85 1.1519930688119508 1106 86-87 1.1732760681431316 1106 88-89 1.056506846873571 1106 90-91 1.0113974296949877 1106 92-93 1.209573314269445 1106 94-95 1.2844603472392393 1106 96-97 0.9918616446374209 1106 98-99 1.1634088023439269 1106 100-101 0.4963594464875847 1107 1 -0.14009409234174086 1107 2 -0.14330504598862603 1107 3 -0.012304304714191971 1107 4 -0.02333823263008128 1107 5 -0.023135118880773575 1107 6 0.002327152173329239 1107 7 -0.04833035214540615 1107 8 0.057178436992288084 1107 9 -0.07889710520590398 1107 10-11 0.029965657569626103 1107 12-13 0.16204330808737666 1107 14-15 0.05877696112137443 1107 16-17 -0.033283684923652856 1107 18-19 0.027832432632557413 1107 20-21 -0.044656957816549436 1107 22-23 0.01746268108842486 1107 24-25 -0.08767676088164222 1107 26-27 -0.10070427351933375 1107 28-29 0.03182149721830285 1107 30-31 0.00932211194099608 1107 32-33 -0.09688127564627536 1107 34-35 -0.16860181358188697 1107 36-37 0.01320540764127287 1107 38-39 0.051854302654582796 1107 40-41 0.038322737159113274 1107 42-43 -0.1653794173922094 1107 44-45 -0.32752636697995996 1107 46-47 -0.3922484991470512 1107 48-49 -0.4244076330899986 1107 50-51 -0.4385098686835036 1107 52-53 -0.3621604318498157 1107 54-55 -0.5193163837689667 1107 56-57 -0.5576230461178682 1107 58-59 -0.525620224369959 1107 60-61 -0.44823776829017703 1107 62-63 -0.5577328523496377 1107 64-65 -0.5269999048808955 1107 66-67 -0.5662825278225121 1107 68-69 -0.735126772718683 1107 70-71 -0.755899797346359 1107 72-73 -0.9191953335485721 1107 74-75 -0.9897994523990192 1107 76-77 -1.0428664477838794 1107 78-79 -0.7398116960326995 1107 80-81 -0.5078428738089933 1107 82-83 -0.6249171712643822 1107 84-85 -0.7081951925390975 1107 86-87 -0.7672964196993277 1107 88-89 -0.8961991091185304 1107 90-91 -0.4439567038694605 1107 92-93 -0.25555500197110703 1107 94-95 -0.4369634093210202 1107 96-97 -0.41931607215202504 1107 98-99 -0.5802476086532344 1107 100-101 -0.9760272837353412 1108 1 -0.08410146106897542 1108 2 0.016660049513959052 1108 3 -0.06721853739897199 1108 4 -0.01564598582125143 1108 5 -0.008886406528262114 1108 6 0.05427628341938373 1108 7 0.05913454053909106 1108 8 0.03091497927046305 1108 9 0.02058763792746987 1108 10-11 0.06953348686209182 1108 12-13 0.07558195927786926 1108 14-15 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sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 15.0 5 51.0 6 128.0 7 224.0 8 455.0 9 869.0 10 1672.0 11 2671.0 12 4330.0 13 6266.0 14 7414.0 15 10139.0 16 14105.0 17 21595.0 18 20616.0 19 10829.0 20 8379.0 21 8295.0 22 8567.0 23 9318.0 24 10114.0 25 11394.0 26 12679.0 27 14139.0 28 16415.0 29 19619.0 30 23704.0 31 27890.0 32 34652.0 33 44436.0 34 63354.0 35 97599.0 36 137588.0 37 233761.0 38 164329.0 39 7942.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.81648850401401 23.217620360866178 16.28768456410954 18.67820657101027 2 14.42308238865131 24.399038233806195 40.714105714702555 20.463773662839937 3 18.385915316827685 29.798078362344842 32.96209208778674 18.853914233040722 4 10.906932636577052 20.34497393795509 40.88264216651288 27.86545125895498 5 9.963261137711049 41.353862127898225 35.383654901009706 13.299221833381017 6 24.342954153158804 41.77382957639467 18.751124997394744 15.13209127305179 7 22.63068443420447 38.48722666115931 22.88941015083965 15.992678753796572 8 23.831471127119492 34.90419285344813 21.355624229080732 19.908711790351642 9 25.281888820889048 18.538251806153713 24.165607195435967 32.01425217752127 10-11 22.236769556954712 29.491036968124916 28.468591967471234 19.80360150744914 12-13 24.669984974771637 28.80604381758274 28.95965925131542 17.564311956330204 14-15 21.36384887681724 29.301041779660736 30.410416565021347 18.924692778500678 16-17 16.895121656604605 32.42462927479732 31.020977563457308 19.659271505140772 18-19 16.505772302422038 30.365834942276976 35.99626927179747 17.132123483503513 20-21 18.379473226483054 28.50056557763761 35.81158022581422 17.30838097006512 22-23 17.71532213293822 28.98632761060974 35.626512233340094 17.671838023111945 24-25 17.737231498266553 29.646352421684675 35.980594122626314 16.635821957422458 26-27 17.45269835615478 28.920108042250547 37.172161782943455 16.455031818651214 28-29 16.392388094626817 30.18799553221664 36.563728912792016 16.855887460364528 30-31 17.179160216681602 29.294837422481756 36.336136905788216 17.18986545504842 32-33 17.07288190026687 29.805638160705676 36.25772933152828 16.86375060749917 34-35 17.705806191205085 29.528532100834127 36.075520896252804 16.690140811707984 36-37 17.88707795906029 28.937303543490273 36.09725195425957 17.078366543189873 38-39 16.891162006197113 30.327290989548295 35.67599708148162 17.105549922772973 40-41 16.884606984986018 30.26843065442733 35.93161018125453 16.915352179332125 42-43 16.766892573681915 30.291942102365915 35.02561469099881 17.91555063295337 44-45 17.353764443785472 29.956338828126 34.672901224947644 18.016995503140883 46-47 18.150040384373973 30.688097623598686 33.63201189977096 17.529850092256385 48-49 17.760445656061336 30.587126281059856 33.618942277402894 18.033485785475914 50-51 16.94140495476292 31.1297901567903 33.725166974563024 18.203637913883757 52-53 17.54792252060048 32.11505194409023 32.468230073572464 17.868795461736823 54-55 17.327849346033094 31.140969989332657 32.61033500764525 18.920845656989005 56-57 17.88167964242609 31.403295697631016 32.150246599428925 18.564778060513966 58-59 18.188643673587325 30.94293415187559 32.36126703520016 18.507155139336923 60-61 17.70716585869132 31.514307179690746 32.782972168194036 17.9955547934239 62-63 17.241797464337058 31.20911758645436 32.80317940774531 18.745905541463276 64-65 17.030681042498898 31.790351972934705 32.373618159364824 18.80534882520157 66-67 17.328939340541073 31.139273844847903 31.672559429747277 19.859227384863747 68-69 17.010786408272896 30.938422256855784 32.177556501925636 19.87323483294568 70-71 17.719688411100297 31.122321616775967 31.914841209503038 19.243148762620702 72-73 17.78481144569981 30.084846119303727 32.64425071857728 19.486091716419182 74-75 16.994850117189202 31.852536762546446 31.270143374404817 19.882469745859535 76-77 17.082150019675797 30.774278277443003 31.848529530293618 20.295042172587582 78-79 17.124899090009816 30.745473736872224 31.839470682094685 20.290156491023275 80-81 17.45801594335462 30.48128012944916 31.46526998496991 20.5954339422263 82-83 17.128570860151694 30.657860581796548 31.801710564459746 20.411857993592015 84-85 16.880929328374187 30.546876628285702 31.409027263305283 21.163166780034825 86-87 16.926986276557106 30.571242696588723 31.423564187987747 21.07820683886643 88-89 17.30823655205118 30.12060857459091 30.948986906047836 21.62216796731007 90-91 17.146302250803856 29.596834034133067 31.42825205959017 21.828611655472898 92-93 17.628164440040244 30.034256762773815 31.28411702625531 21.05346177093064 94-95 17.34652067746191 29.43738261534781 31.29166341009372 21.924433297096556 96-97 17.040134222847065 29.2779269353271 31.363601052713353 22.318337789112487 98-99 17.22972668919191 29.53603146776132 30.452941173684213 22.781300669362558 100-101 17.3091880574674 28.41486232361175 31.462169190615363 22.81378042830549 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7424.0 1 4709.0 2 2308.0 3 2466.5 4 2008.5 5 1907.5 6 3075.5 7 5753.5 8 8336.0 9 8447.0 10 6252.5 11 4444.0 12 4072.0 13 4119.5 14 4232.5 15 4242.0 16 4247.0 17 4346.0 18 4540.0 19 4862.0 20 5221.5 21 5760.5 22 6432.0 23 7067.0 24 7738.0 25 9221.5 26 12794.5 27 17078.0 28 18487.0 29 19655.5 30 23091.5 31 26370.5 32 30379.5 33 34988.5 34 39882.0 35 42661.5 36 44512.5 37 47576.0 38 45237.5 39 40309.0 40 37699.0 41 35791.5 42 33902.5 43 32572.5 44 31587.0 45 30008.0 46 28795.0 47 32221.5 48 43538.5 49 41509.0 50 27638.0 51 20261.5 52 15490.0 53 12398.0 54 10396.0 55 8872.0 56 7328.5 57 6330.0 58 5595.0 59 4710.0 60 3846.0 61 3019.5 62 2385.0 63 1717.5 64 1111.5 65 789.0 66 610.0 67 528.5 68 402.5 69 318.0 70 271.0 71 222.0 72 197.0 73 202.5 74 178.5 75 143.0 76 107.0 77 73.0 78 60.0 79 54.0 80 52.5 81 41.5 82 22.5 83 8.0 84 3.5 85 2.0 86 2.5 87 1.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.41352535815180214 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.052612930791108E-4 16-17 0.002415783879237332 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.4210493407278426E-4 26-27 0.004263148022183528 28-29 9.473662271518951E-5 30-31 0.0 32-33 9.473662271518951E-5 34-35 0.013405232114199313 36-37 0.02832625019184166 38-39 0.021599949979063206 40-41 0.010278923564598062 42-43 0.010326291875955655 44-45 0.004121043088110743 46-47 0.007152615014996807 48-49 0.0033631501063892274 50-51 7.578929817215161E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0021789423224493586 60-61 0.00369472828589239 62-63 0.0014684176520854374 64-65 0.0034578867291044173 66-67 0.002463152190594927 68-69 0.0059684072310569386 70-71 4.7368311357594754E-5 72-73 0.0 74-75 0.0 76-77 0.09123136767472749 78-79 0.0153473328798607 80-81 8.052612930791108E-4 82-83 0.0 84-85 0.0 86-87 0.1027892356459806 88-89 0.41186746725428636 90-91 0.41475693424709964 92-93 0.0 94-95 4.7368311357594754E-5 96-97 0.0 98-99 1.4210493407278426E-4 100-101 2.842098681455685E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1055558.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.19616294511174 #Duplication Level Percentage of deduplicated Percentage of total 1 73.01948172260181 25.700055768763015 2 13.326167016018392 9.380598914591134 3 5.104022160865988 5.389259869479019 4 2.4670454851567456 3.473221395543162 5 1.3985693213415578 2.4612136861985903 6 0.893704703568481 1.8872985822965422 7 0.5976545322279934 1.472460241082669 8 0.4203088854918545 1.1834608016839698 9 0.3185056463494623 1.0089158965068428 >10 1.9572026644494176 13.714133362910747 >50 0.27519237721366685 6.735451669774441 >100 0.19656509556676585 13.269086305371033 >500 0.016425302505478 3.91521018657874 >1k 0.007808750371456753 5.2409720790083005 >5k 8.078017625644917E-4 2.425303637798619 >10k+ 5.385345083763277E-4 2.7433576024132162 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16686 1.580775286625652 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12261 1.1615657311109384 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9409 0.8913768831272181 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8162 0.7732403146013767 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8020 0.7597877141758198 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4364 0.413430621529087 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3897 0.3691886187210935 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3382 0.3203992580227709 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3347 0.31708347622773925 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2622 0.24839942475922686 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2533 0.239967865337575 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2156 0.20425215857394857 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2109 0.19979953730633465 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2079 0.196957438624879 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2048 0.1940206033207081 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1908 0.18075747614058157 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1865 0.1766838013638284 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1759 0.16664171935601835 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1755 0.1662627728651576 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1735 0.1643680404108538 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1699 0.16095752199310698 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1577 0.14939965402185384 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1444 0.13679968320073363 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1419 0.1344312676328539 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1346 0.12751549417464508 No Hit CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT 1303 0.12344181939789192 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1266 0.11993656435742991 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1232 0.11671551918511346 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1166 0.11046290208591096 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1154 0.1093260626133287 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1101 0.10430502160942365 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 9.473662271518951E-5 0.0 18-19 0.0 0.0 0.0 1.4210493407278426E-4 0.0 20-21 0.0 0.0 0.0 5.210514249335423E-4 0.0 22-23 0.0 0.0 0.0 0.001326312718012653 0.0 24-25 0.0 0.0 0.0 0.003742096597249985 0.0 26-27 0.0 0.0 0.0 0.009189452403373382 0.0 28-29 0.0 0.0 0.0 0.022973631008433457 0.0 30-31 0.0 0.0 0.0 0.056889341940471294 0.0 32-33 0.0 0.0 0.0 0.11718920229868943 0.0 34-35 0.0 0.0 0.0 0.2082310967279865 0.0 36-37 0.0 0.0 0.0 0.33148344288044806 0.0 38-39 0.0 0.0 0.0 0.5046146208924569 0.0 40-41 0.0 0.0 0.0 0.6984931192790922 0.0 42-43 0.0 0.0 0.0 0.8865453153687434 0.0 44-45 0.0 0.0 0.0 1.1078974343427836 0.0 46-47 0.0 0.0 0.0 1.3723547166522352 0.0 48-49 0.0 0.0 0.0 1.6458119781196296 0.0 50-51 0.0 0.0 0.0 1.9467902284857868 0.0 52-53 0.0 0.0 0.0 2.2278264197703965 0.0 54-55 0.0 0.0 0.0 2.5237362608212908 0.0 56-57 0.0 0.0 0.0 2.8912669886448685 0.0 58-59 0.0 0.0 0.0 3.2810134544951577 0.0 60-61 0.0 0.0 0.0 3.723338745952378 0.0 62-63 0.0 0.0 0.0 4.157516687856091 0.0 64-65 0.0 0.0 0.0 4.609647219764334 0.0 66-67 0.0 0.0 0.0 5.110993427173116 0.0 68-69 0.0 0.0 0.0 5.675576330244288 0.0 70-71 0.0 0.0 0.0 6.30396434871414 0.0 72-73 0.0 0.0 0.0 6.915678721586119 0.0 74-75 0.0 0.0 0.0 7.569645628189072 0.0 76-77 0.0 0.0 0.0 8.244359855166651 0.0 78-79 0.0 0.0 0.0 9.009737030082668 0.0 80-81 0.0 0.0 0.0 9.835461433668259 0.0 82-83 0.0 0.0 0.0 10.645270084637698 0.0 84-85 0.0 0.0 0.0 11.460099776611044 0.0 86-87 0.0 0.0 0.0 12.309271494318644 0.0 88-89 0.0 0.0 0.0 13.228927259326348 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTTAG 35 2.745161E-4 54.45553 1 GTAAGCG 130 0.0 36.505173 94-95 CGGAGAT 70 2.015887E-4 34.034706 1 ACAACGT 30 0.0039693653 31.637817 94-95 GTCGAAT 95 3.513495E-5 29.971249 7 GGGCGCA 65 0.0058699427 29.20414 9 CTCCGAC 245 0.0 27.11813 94-95 CATTCCG 280 0.0 27.11813 9 CTAGTCG 115 1.2900263E-4 24.758858 4 TAGTCGA 120 1.7218442E-4 23.727238 5 GTACATG 7765 0.0 22.949867 1 AGTACTT 10820 0.0 22.874174 12-13 GAGTACT 10755 0.0 22.703527 12-13 TACATGG 7880 0.0 22.462587 2 CATGGGG 2220 0.0 22.444687 4 CATATAG 365 0.0 22.102085 3 TCGTATC 65 2.9608276E-4 21.903105 94-95 ATGGGAT 1585 0.0 21.556608 5 ACATGGG 7710 0.0 21.542242 3 ATGGGTA 470 0.0 21.203066 5 >>END_MODULE