FastQCFastQC Report
Fri 27 May 2016
SRR939073_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939073_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3286277
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135640.41274670394492No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102290.3112640839466667No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69350.211029076368182No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76900.040.3182871
GTATCAA123600.037.9152451
ATCAACG154600.030.039363
TATCAAC157000.029.6707152
TCAACGC156500.029.6141054
CAACGCA157200.029.452095
AACGCAG161900.028.675296
ACGCAGA185600.024.9115317
CGCAGAG187350.024.6282658
GTACATG117300.023.7604851
TACATGG117950.023.1418822
GCAGAGT204450.022.4283319
ACATGGG120550.021.8958633
GAGTACT116250.021.23065612-13
AGAGTAC172300.020.7435310-11
GTACTTT120050.020.4809614-15
AGTACTT120050.019.41429312-13
TAAGGTG13700.019.0246325
CAGAGTA198800.018.94346810-11
CATGGGG74850.018.3603234
Produced by FastQC (version 0.11.5)