FastQCFastQC Report
Fri 27 May 2016
SRR939073_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939073_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3286277
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142450.4334692419415649No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113290.3447366122819227No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80060.24361914713823574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83650.044.2772061
GTATCAA135750.036.927941
TATCAAC174250.028.785842
ATCAACG173800.028.6965483
TCAACGC176100.028.2948024
CAACGCA177600.028.0825465
AACGCAG183900.027.1979146
ACGCAGA206950.024.0539687
CGCAGAG210150.023.5522088
GAGTACT132850.022.02156612-13
GCAGAGT228500.021.3492989
GTACTTT138950.020.88404514-15
GTACATG115100.020.680511
GTAAGGT13250.020.4143544
AGTACTT137400.020.30800612-13
AGAGTAC194150.020.1769910-11
TAAGGTG14350.020.1722645
TACATGG116700.020.1284852
CAGAGTA221350.018.99463310-11
ACATGGG120600.018.7299393
Produced by FastQC (version 0.11.5)