##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939073_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3286277 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.22530510970317 33.0 31.0 34.0 31.0 34.0 2 32.470506290248814 34.0 31.0 34.0 31.0 34.0 3 32.55683315800829 34.0 31.0 34.0 31.0 34.0 4 36.10679653601933 37.0 35.0 37.0 35.0 37.0 5 36.052209232514485 37.0 35.0 37.0 35.0 37.0 6 36.08894076792674 37.0 35.0 37.0 35.0 37.0 7 36.03744297878724 37.0 35.0 37.0 35.0 37.0 8 36.01516792406726 37.0 35.0 37.0 35.0 37.0 9 37.71594816870276 39.0 37.0 39.0 35.0 39.0 10-11 37.62854196405233 39.0 37.0 39.0 35.0 39.0 12-13 37.54342360671362 39.0 37.0 39.0 35.0 39.0 14-15 38.80130463743622 40.0 38.0 41.0 35.0 41.0 16-17 38.70740917457658 40.0 38.0 41.0 34.0 41.0 18-19 38.731337315752754 40.0 38.0 41.0 34.5 41.0 20-21 38.81941434030059 40.0 38.0 41.0 35.0 41.0 22-23 38.76562763881438 40.0 38.0 41.0 34.0 41.0 24-25 38.688742610559004 40.0 38.0 41.0 34.0 41.0 26-27 38.51664330182757 40.0 38.0 41.0 34.0 41.0 28-29 38.31864781940171 40.0 38.0 41.0 34.0 41.0 30-31 38.07132767566459 40.0 37.5 41.0 33.0 41.0 32-33 37.88357706912716 40.0 37.0 41.0 33.0 41.0 34-35 37.8022003318649 40.0 37.0 41.0 33.0 41.0 36-37 37.6608441710787 40.0 37.0 41.0 33.0 41.0 38-39 37.483319269799836 40.0 37.0 41.0 32.5 41.0 40-41 37.257230750785766 40.0 36.0 41.0 31.5 41.0 42-43 37.04625431757579 40.0 36.0 41.0 31.0 41.0 44-45 36.772187189333096 39.0 36.0 41.0 31.0 41.0 46-47 36.42925079048419 39.0 35.0 41.0 30.0 41.0 48-49 36.039207741769786 39.0 35.0 40.5 29.5 41.0 50-51 35.70620994517504 39.0 35.0 40.0 28.0 41.0 52-53 35.403357507599026 38.0 35.0 40.0 27.5 41.0 54-55 35.096913619880496 38.0 34.0 40.0 27.0 41.0 56-57 34.745307988340606 38.0 34.0 40.0 26.0 41.0 58-59 34.365099624894675 37.5 34.0 40.0 26.0 41.0 60-61 33.92668420830015 37.0 33.0 40.0 24.5 41.0 62-63 33.44619930091103 36.0 33.0 39.0 23.0 40.5 64-65 32.945532284710026 36.0 32.0 39.0 22.0 40.0 66-67 32.53472850888711 35.0 31.5 38.5 21.5 40.0 68-69 31.992241524375455 35.0 31.0 38.0 19.5 40.0 70-71 31.45137567527022 35.0 31.0 37.0 18.0 39.5 72-73 30.89064951006869 34.5 30.0 37.0 13.5 39.0 74-75 30.320946469211208 34.0 29.0 36.0 8.0 39.0 76-77 28.44594232318213 32.0 27.0 34.5 7.0 36.5 78-79 29.434327355849796 34.0 29.0 35.0 7.0 37.0 80-81 29.458040968548907 34.0 29.0 35.0 2.0 37.0 82-83 29.11496702803811 34.0 29.0 35.0 2.0 36.5 84-85 28.648157474248215 34.0 29.0 35.0 2.0 36.0 86-87 27.959871611553133 33.0 27.0 35.0 2.0 35.5 88-89 27.29261136538399 33.0 26.0 35.0 2.0 35.0 90-91 26.691784046201825 32.5 25.0 35.0 2.0 35.0 92-93 26.25412784740909 32.0 24.5 34.0 2.0 35.0 94-95 25.72804164104243 32.0 23.5 34.0 2.0 35.0 96-97 24.922306001593903 32.0 19.0 34.0 2.0 35.0 98-99 23.829698470335885 31.0 2.0 34.0 2.0 35.0 100-101 20.143232904590818 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18904019540693895 1101 2 0.2042962387125158 1101 3 0.2121256578412556 1101 4 0.18072544173662664 1101 5 0.1602812374237459 1101 6 0.17030241415368863 1101 7 0.17430815313963421 1101 8 0.18174357618856618 1101 9 0.2570374327751139 1101 10-11 0.2149526433689246 1101 12-13 0.21321713783523677 1101 14-15 0.2962856442325972 1101 16-17 0.27880489671866115 1101 18-19 0.2771603330116861 1101 20-21 0.31948788101176007 1101 22-23 0.2905135011176796 1101 24-25 0.2745425805086654 1101 26-27 0.24519378529069513 1101 28-29 0.1830951114295658 1101 30-31 0.19402513672559962 1101 32-33 0.29084172976862277 1101 34-35 0.4741996872721117 1101 36-37 0.5529641349430534 1101 38-39 0.5518930366261827 1101 40-41 0.4615649432046425 1101 42-43 0.4735413553251746 1101 44-45 0.5074631191951156 1101 46-47 0.44950418089969446 1101 48-49 0.2905530585420948 1101 50-51 0.48924070054125224 1101 52-53 0.492147607449148 1101 54-55 0.45308129221456284 1101 56-57 0.22731201638005416 1101 58-59 0.4590483593553216 1101 60-61 0.6408714458036187 1101 62-63 0.43513415747772655 1101 64-65 0.3562283084979754 1101 66-67 0.3644825010713646 1101 68-69 0.33046586050033255 1101 70-71 0.24161276781573449 1101 72-73 0.25878076556502805 1101 74-75 0.24066214744191683 1101 76-77 0.3649384631714092 1101 78-79 0.38172934429272587 1101 80-81 0.36224554842896595 1101 82-83 0.4145758309316854 1101 84-85 0.3482351171884659 1101 86-87 0.2799095412279051 1101 88-89 0.3341734632152864 1101 90-91 0.5002562040691636 1101 92-93 0.43779353022973666 1101 94-95 0.3416237566316376 1101 96-97 0.39592871645526984 1101 98-99 0.034554027722894176 1101 100-101 -0.1872438040450497 1102 1 0.11813754952603261 1102 2 0.05778978673246371 1102 3 0.1315776085515452 1102 4 0.07666147679214674 1102 5 0.09783936837688856 1102 6 0.09428302175664527 1102 7 0.10221587897671469 1102 8 0.05788407659048289 1102 9 0.18297359676353153 1102 10-11 0.19712355770768397 1102 12-13 0.14685192962193838 1102 14-15 0.19590254680200303 1102 16-17 0.2250684435648722 1102 18-19 0.19307768835786732 1102 20-21 0.18713220360721294 1102 22-23 0.25089647850729335 1102 24-25 0.24140276878626565 1102 26-27 0.19637834517602215 1102 28-29 0.03533945527670568 1102 30-31 0.15732322024921785 1102 32-33 0.13115450097230053 1102 34-35 0.2205110393247267 1102 36-37 0.2651705124977042 1102 38-39 0.2829198382189091 1102 40-41 0.15565108016161133 1102 42-43 0.14913267938600683 1102 44-45 0.19157951981541288 1102 46-47 0.21813648517066042 1102 48-49 0.12193478128124013 1102 50-51 0.09149882687059119 1102 52-53 0.18007369733226852 1102 54-55 0.04518873155385705 1102 56-57 0.17822603947770688 1102 58-59 0.12587269094939302 1102 60-61 0.11737425876377472 1102 62-63 0.09881430474576547 1102 64-65 0.07240876476037528 1102 66-67 0.18585575202990867 1102 68-69 0.055684679635042755 1102 70-71 -0.12880705770693268 1102 72-73 -0.19955134512875716 1102 74-75 -0.1927910823716381 1102 76-77 -0.10628854898207152 1102 78-79 -0.11493740684721487 1102 80-81 -0.07139229995661722 1102 82-83 0.11438664413370248 1102 84-85 0.12271525085186852 1102 86-87 0.18509178787920533 1102 88-89 0.043723267326203796 1102 90-91 0.19449350373411534 1102 92-93 0.3857094744275784 1102 94-95 0.1685604621382275 1102 96-97 -0.022588470744629774 1102 98-99 -0.21086046264537472 1102 100-101 -0.5800561061997378 1103 1 0.042236677844805115 1103 2 0.06600606606316717 1103 3 0.08305111273813992 1103 4 0.06645095490544861 1103 5 0.06645283946343739 1103 6 0.05863363501909902 1103 7 0.09331038065275976 1103 8 0.052861434267306606 1103 9 0.11417200943411387 1103 10-11 0.11359963906927106 1103 12-13 0.10224072364429304 1103 14-15 0.16562041188426946 1103 16-17 0.04681668863142363 1103 18-19 0.07641417011077323 1103 20-21 0.1277799292064472 1103 22-23 0.1840684242919437 1103 24-25 0.16076469444440278 1103 26-27 0.12481330744758878 1103 28-29 0.15074977835276115 1103 30-31 0.13226096043491964 1103 32-33 0.09755030859232505 1103 34-35 0.2557130363723772 1103 36-37 0.32551486778319116 1103 38-39 0.18629977254477126 1103 40-41 0.20856710107354814 1103 42-43 0.22252705115138838 1103 44-45 0.29599411539178533 1103 46-47 0.20408901869147655 1103 48-49 0.1622762262874815 1103 50-51 0.11633898065956316 1103 52-53 0.06342047901924985 1103 54-55 0.10464871696739664 1103 56-57 0.2621238238084871 1103 58-59 0.2378343988625531 1103 60-61 0.22615547852141304 1103 62-63 0.07963462217659156 1103 64-65 -0.007809751628357731 1103 66-67 0.0037415218163019404 1103 68-69 -0.006640988501963818 1103 70-71 -0.07708089832881981 1103 72-73 -0.19985486446282863 1103 74-75 -0.3322192081167401 1103 76-77 -0.15452500303472405 1103 78-79 0.021113107535871478 1103 80-81 0.031533735997609114 1103 82-83 -0.25185501511116826 1103 84-85 -0.18717031907197423 1103 86-87 -0.013405583226980156 1103 88-89 -0.18156949186396787 1103 90-91 0.0399907670197166 1103 92-93 -0.11915439550748275 1103 94-95 -0.28856156972757674 1103 96-97 -0.258792037765204 1103 98-99 -0.321399903065295 1103 100-101 -0.6124040353432036 1104 1 0.1657665203164953 1104 2 0.156059724471298 1104 3 0.20050250213401455 1104 4 0.11316821772074803 1104 5 0.1207227003857625 1104 6 0.16408119555581635 1104 7 0.15613736927129906 1104 8 0.09292358150710811 1104 9 0.12521476980949586 1104 10-11 0.11630659154017309 1104 12-13 0.1063807685997773 1104 14-15 0.16942422203160135 1104 16-17 0.20630706328113746 1104 18-19 0.22302678372248863 1104 20-21 0.21439116833394678 1104 22-23 0.21969630773825344 1104 24-25 0.2133645677435112 1104 26-27 0.25524971903324456 1104 28-29 0.14417848062342387 1104 30-31 0.27672685235206984 1104 32-33 0.29529603671891635 1104 34-35 0.293336919256042 1104 36-37 0.32556748141203684 1104 38-39 0.29562607616919934 1104 40-41 0.4624796175079453 1104 42-43 0.35913686655302257 1104 44-45 0.2738473102005159 1104 46-47 0.3486606195780766 1104 48-49 0.2845076949966625 1104 50-51 0.16747150641562314 1104 52-53 0.12047971864687668 1104 54-55 0.2145433334210054 1104 56-57 0.2694426846650799 1104 58-59 0.2743926818106175 1104 60-61 0.26438749573971876 1104 62-63 0.1826130888045583 1104 64-65 0.16699252710377266 1104 66-67 0.01604593881340577 1104 68-69 -0.025908013747724112 1104 70-71 -0.056624045037025894 1104 72-73 0.04560339649777134 1104 74-75 -0.017687655675349134 1104 76-77 0.13034787751382026 1104 78-79 0.1298871553897598 1104 80-81 0.14108564129606904 1104 82-83 0.28871796068784406 1104 84-85 0.13196912749045708 1104 86-87 0.19127995212499016 1104 88-89 0.2067480452487125 1104 90-91 0.3408440605258356 1104 92-93 0.24844905423381647 1104 94-95 0.13322824423105928 1104 96-97 0.1643471578789253 1104 98-99 0.019868257735655703 1104 100-101 -0.2612362696957611 1105 1 0.020668221246914698 1105 2 0.04721772347330955 1105 3 0.03900412209737425 1105 4 0.018952447146219242 1105 5 0.08565602077764112 1105 6 0.018417641060466394 1105 7 0.06225135695285644 1105 8 0.05990295855021088 1105 9 0.0564599660198013 1105 10-11 0.08840665803104741 1105 12-13 0.05217846626496936 1105 14-15 -0.0016687233401029289 1105 16-17 0.0927720215926442 1105 18-19 0.1103377067677016 1105 20-21 0.07035654703538086 1105 22-23 0.06647532656848654 1105 24-25 -0.0012823399743666641 1105 26-27 -0.03485645482941635 1105 28-29 0.037908594022937336 1105 30-31 0.03354113042828999 1105 32-33 0.00869115928321662 1105 34-35 0.09257060462186928 1105 36-37 -0.01478685109296407 1105 38-39 0.04426654924578344 1105 40-41 0.11362649183520546 1105 42-43 0.15096410889878342 1105 44-45 0.09688015702635511 1105 46-47 -0.01702759834608969 1105 48-49 0.01821753344830057 1105 50-51 0.0478582838146977 1105 52-53 -0.0336728295963411 1105 54-55 0.013601614355948755 1105 56-57 -0.13180332453413257 1105 58-59 0.04258584921955588 1105 60-61 0.1049741626386691 1105 62-63 0.012144402772996443 1105 64-65 -0.10338808858600146 1105 66-67 -0.049350152700938565 1105 68-69 -0.08479957181193498 1105 70-71 -0.05815659134732343 1105 72-73 -0.08484445876697322 1105 74-75 -0.15801472609055978 1105 76-77 -0.1457119427077842 1105 78-79 0.08573776162646496 1105 80-81 -0.18542091840448194 1105 82-83 -0.16174524596439355 1105 84-85 0.020483823716734406 1105 86-87 -0.10629104648670662 1105 88-89 0.11971623456894065 1105 90-91 -0.07779996315537119 1105 92-93 -0.16291840762725585 1105 94-95 -0.4007319823605613 1105 96-97 -0.4151928666849578 1105 98-99 -0.3899756276544757 1105 100-101 -0.6765514460002855 1106 1 0.014193784157910727 1106 2 0.08390162603015483 1106 3 0.08851796753059205 1106 4 0.00869965409034279 1106 5 0.07917609455164154 1106 6 0.0750545566046057 1106 7 0.054600414833977595 1106 8 0.040229891549806496 1106 9 0.15512903326904848 1106 10-11 0.15682234671647421 1106 12-13 0.13622813197652306 1106 14-15 0.02528065217907738 1106 16-17 -0.04361748024987122 1106 18-19 -0.05878717841785175 1106 20-21 0.058688928918300576 1106 22-23 0.13648423726754544 1106 24-25 0.13789289889457734 1106 26-27 0.09566241499387473 1106 28-29 0.18119702621562794 1106 30-31 0.171984484364188 1106 32-33 0.13564803662296043 1106 34-35 -0.10808617061993431 1106 36-37 0.12382986808644603 1106 38-39 0.20345197961242434 1106 40-41 0.010606368658969245 1106 42-43 0.06021861090598435 1106 44-45 0.21163788481639045 1106 46-47 0.13452198502841384 1106 48-49 0.13113274091937654 1106 50-51 0.13092219200704847 1106 52-53 0.257607310340056 1106 54-55 0.1652070039565956 1106 56-57 0.17929259996461155 1106 58-59 0.0852378159773366 1106 60-61 0.03316069815155487 1106 62-63 0.13615635811337512 1106 64-65 0.19639015027928508 1106 66-67 0.16836869004260535 1106 68-69 0.12549612799814014 1106 70-71 0.15538273100041877 1106 72-73 -0.06115197119986959 1106 74-75 0.033204850367628325 1106 76-77 0.21084501440741477 1106 78-79 0.32005849922009943 1106 80-81 0.16902500810694931 1106 82-83 0.08011207672733534 1106 84-85 0.10334188927166821 1106 86-87 0.14187237975941613 1106 88-89 0.28782014027288483 1106 90-91 0.31514036626603925 1106 92-93 0.3611070875843829 1106 94-95 -0.004409887120377931 1106 96-97 0.14216998722715246 1106 98-99 0.263723867710862 1106 100-101 -0.44171335724459837 1107 1 0.010069269022345395 1107 2 -0.05580410417906023 1107 3 -0.003461658792055289 1107 4 0.05691465826113529 1107 5 0.036057808371545264 1107 6 0.0220289175781474 1107 7 0.09676469072727656 1107 8 0.05875052587068552 1107 9 0.061578948478690165 1107 10-11 0.11873510535456688 1107 12-13 0.11485071465150298 1107 14-15 0.07139401487041397 1107 16-17 0.15413013984731805 1107 18-19 0.11348694760166467 1107 20-21 0.15026781894172814 1107 22-23 0.09480581290389978 1107 24-25 0.010523679220817428 1107 26-27 0.04183525338938665 1107 28-29 0.14647845875043686 1107 30-31 0.14303286110228441 1107 32-33 0.1854709339693983 1107 34-35 0.1728972976332912 1107 36-37 0.18554250847284948 1107 38-39 0.22670423106769988 1107 40-41 0.10150128808438552 1107 42-43 0.1768781055024462 1107 44-45 0.09691724819645486 1107 46-47 0.18801038501702294 1107 48-49 0.12546795498443686 1107 50-51 -0.037632803277375615 1107 52-53 0.006158976984231401 1107 54-55 0.016207720164032935 1107 56-57 0.003224473794155358 1107 58-59 -0.18857058329155763 1107 60-61 -0.04411018669198796 1107 62-63 -0.16348293923365276 1107 64-65 -0.09035886274889293 1107 66-67 0.001872903571381812 1107 68-69 -0.2494234673471567 1107 70-71 -0.172353988549645 1107 72-73 -0.2296321970276196 1107 74-75 -0.18754912163480597 1107 76-77 -0.12514308445419786 1107 78-79 0.08748470561478783 1107 80-81 0.0025496632853112544 1107 82-83 -0.10742066614720258 1107 84-85 0.01427872157567478 1107 86-87 -0.05094445456375851 1107 88-89 0.031185325594684343 1107 90-91 5.344327898626489E-4 1107 92-93 -0.02072073574978006 1107 94-95 -0.0668429832006261 1107 96-97 0.025810670008237224 1107 98-99 0.17598690825879615 1107 100-101 -0.3019063683995462 1108 1 -0.0298863817462518 1108 2 -0.008602432746918964 1108 3 0.010864723411202704 1108 4 0.013471685378540599 1108 5 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42-43 -0.4629264395775081 2316 44-45 -0.5692921012113885 2316 46-47 -0.4911053383744388 2316 48-49 -0.4537389070884359 2316 50-51 -0.33552114641673825 2316 52-53 -0.3393641245402037 2316 54-55 -0.3322160272068544 2316 56-57 -0.3652044780216954 2316 58-59 -0.2788857688633115 2316 60-61 -0.04455540625501442 2316 62-63 -0.033818861392695965 2316 64-65 -0.12391693716921282 2316 66-67 -0.04116578591528253 2316 68-69 0.029113393479406113 2316 70-71 0.30811509351535804 2316 72-73 0.3006770777236376 2316 74-75 0.13731058772008353 2316 76-77 0.169659453138987 2316 78-79 0.3420822156816534 2316 80-81 0.4356540335170216 2316 82-83 0.35472690219564385 2316 84-85 0.3230879145253951 2316 86-87 0.1233808654669808 2316 88-89 0.07588554022729 2316 90-91 0.17392538850376837 2316 92-93 0.10160186757692102 2316 94-95 0.5111020648430618 2316 96-97 0.32364838956821984 2316 98-99 0.5898690547797578 2316 100-101 0.5075255311132594 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 16.0 4 33.0 5 93.0 6 198.0 7 466.0 8 1074.0 9 2052.0 10 3481.0 11 5506.0 12 7855.0 13 11040.0 14 13880.0 15 18353.0 16 23917.0 17 32675.0 18 32517.0 19 25209.0 20 24059.0 21 25237.0 22 27446.0 23 29887.0 24 34595.0 25 39510.0 26 46076.0 27 54637.0 28 65291.0 29 78763.0 30 95066.0 31 115236.0 32 143103.0 33 181392.0 34 243899.0 35 343912.0 36 499574.0 37 658661.0 38 381404.0 39 20161.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.15154702807844 19.6363496498971 15.475531259558458 22.736572062466003 2 16.2575461532914 20.730571403445296 39.235493538736996 23.776388904526307 3 19.77030542464923 24.16610042306233 29.697557448748235 26.366036703540207 4 14.114178445700103 17.28573093503682 35.685366753928534 32.91472386533454 5 14.509975878478897 36.667511594427374 33.60647930773943 15.216033219354303 6 32.655311770736304 35.63734889055305 16.885582073574444 14.8217572651362 7 29.03337119786311 32.3876228327679 20.185456064720046 18.39354990464894 8 26.344249130551077 35.232300868125236 19.277285511842123 19.146164489481563 9 26.379790869728875 17.199006657077295 20.077309368625958 36.343893104567876 10-11 25.36975732721253 26.457294987610602 26.512539874149375 21.660407811027493 12-13 26.249354512720625 23.733559283042787 27.30033104330524 22.716755160931353 14-15 23.007061867951865 25.126976384263966 25.80387439630873 26.062087351475437 16-17 22.463716737680244 28.191046507452622 26.578847589910126 22.766389164957005 18-19 22.490761579368257 26.45143012942309 29.10934748024816 21.94846081096049 20-21 23.033481352910908 25.71800855496965 29.26784625885158 21.980663833267858 22-23 22.81846600271371 25.511163544643377 29.291566109612795 22.378804343030122 24-25 22.62453800148862 25.778512755814653 29.282291816913176 22.314657425783547 26-27 22.38964750617879 26.074616537674338 29.170706235268128 22.36502972087874 28-29 22.412900677575262 26.293690397979237 29.150540261822115 22.14286866262339 30-31 22.541019836226482 25.93317569357411 29.00159744530473 22.524207024894675 32-33 22.442036992012543 26.005446284655857 28.990298139809884 22.562218583521716 34-35 22.928493270790007 26.124219419401264 28.76037889362101 22.18690841618772 36-37 22.99169472530135 25.91164436890444 28.76125211619227 22.335408789601942 38-39 22.649624091730658 26.046061032534485 28.858353734376212 22.445961141358644 40-41 22.835707132719477 26.01272157029116 28.727509722410105 22.42406157457926 42-43 22.66052840202742 26.039206377465234 28.463090526469554 22.837174694037788 44-45 22.779761757138104 26.202774068224105 28.562851538007074 22.45461263663072 46-47 23.109610581674083 26.49143133619729 27.908807842379492 22.490150239749138 48-49 23.041805946282317 26.445163263349926 27.839607699341652 22.673423091026105 50-51 22.947440491133577 26.776091471346735 27.7800853549992 22.496382682520483 52-53 23.241234643900064 26.66252418581461 27.51621098109395 22.58003018919138 54-55 22.92215476662497 26.610492663886827 27.560640810254277 22.90671175923393 56-57 22.948734997080283 26.87166967361546 27.481234844171688 22.698360485132568 58-59 23.060608638005586 26.51441714071568 27.549811092982786 22.875163128295945 60-61 22.907448432285797 26.590253371441076 27.854592276532802 22.647705919740325 62-63 22.763786271463438 26.649880003609095 27.918069845157262 22.668263879770205 64-65 22.593439056642442 26.582536559106902 27.760272860653405 23.063751523597254 66-67 22.63800445626235 26.649507845322297 27.622816525782966 23.08967117263239 68-69 22.727319064585487 26.458262550782756 27.839090956534783 22.97532742809697 70-71 22.76158138245519 26.537298367924915 27.64149638656394 23.059623863055954 72-73 22.715401045012335 26.602337538801507 27.562375295813467 23.11988612037269 74-75 22.719128667486032 26.945537457737128 27.185885425969875 23.149448448806965 76-77 22.797431765745664 26.66454136180205 27.463695630729955 23.074331241722327 78-79 22.699368021238698 26.728759438335093 27.455922231481296 23.11595030894491 80-81 22.767845663469277 26.841403037792176 27.299504788173586 23.09124651056496 82-83 22.778386372350333 26.743136574708025 27.355626152525968 23.12285090041567 84-85 22.77493650109227 26.75866946091276 27.22725442803513 23.239139609959842 86-87 22.815570503489084 26.61258118077303 27.27334340354151 23.298504912196375 88-89 22.637557954385443 26.71166076292239 27.17101961537745 23.47976166731472 90-91 22.612423656822 26.65658301716944 27.288081111023732 23.442912214984833 92-93 22.567954557695533 26.457173269325747 27.34431698849488 23.63055518448384 94-95 22.400424553377576 26.33896959994547 27.486301367778797 23.774304478898156 96-97 22.560700537523243 26.094167669701562 27.474757525728588 23.87037426704661 98-99 22.261534367536573 26.38182892359901 27.20314913715048 24.15348757171394 100-101 22.585266563469094 25.471197744056834 27.784790154622907 24.158745537851164 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1593.0 1 1074.5 2 721.5 3 942.0 4 823.0 5 738.5 6 1367.5 7 3149.0 8 5181.5 9 5654.0 10 4533.0 11 3477.0 12 3255.0 13 3485.5 14 3783.5 15 4035.0 16 4326.5 17 4559.5 18 4835.5 19 5326.5 20 5961.0 21 6942.0 22 8329.5 23 10328.0 24 12746.0 25 15696.5 26 20028.5 27 24730.5 28 28267.0 29 32172.5 30 37009.0 31 42807.0 32 49711.5 33 53596.5 34 56357.0 35 60245.5 36 65461.5 37 71865.5 38 76227.5 39 79860.5 40 84293.5 41 90099.0 42 94868.0 43 98460.0 44 103131.5 45 108338.5 46 110864.5 47 113591.0 48 118984.0 49 120354.0 50 119123.0 51 118432.5 52 119525.0 53 123279.5 54 125138.0 55 118125.5 56 107144.5 57 98593.0 58 91814.0 59 82204.5 60 68199.5 61 56261.5 62 47147.0 63 37207.5 64 27394.5 65 20178.5 66 14979.5 67 11821.0 68 9230.0 69 6869.0 70 5158.5 71 3850.0 72 2850.0 73 2350.5 74 1934.5 75 1437.0 76 1016.5 77 603.5 78 373.0 79 245.5 80 154.0 81 93.5 82 55.0 83 36.0 84 23.5 85 11.0 86 7.0 87 5.5 88 4.5 89 2.5 90 1.5 91 0.5 92 1.0 93 1.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31595023791360255 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0010954645636992865 16-17 0.0015519081319073226 18-19 3.0429571213869068E-5 20-21 0.0 22-23 0.0 24-25 3.3472528335255975E-4 26-27 0.0010650349924854173 28-29 0.0 30-31 0.0028147353372828888 32-33 0.0 34-35 0.010391698569536287 36-37 0.027234466236412815 38-39 0.02134634420652915 40-41 0.013404226119709324 42-43 0.01010261764300453 44-45 0.0028147353372828888 46-47 8.976723508091375E-4 48-49 6.694505667051195E-4 50-51 6.0859142427738136E-5 52-53 4.56443568208036E-5 54-55 0.0 56-57 0.0 58-59 0.004153636470693128 60-61 0.010056973286183728 62-63 0.004153636470693128 64-65 0.0017497003447974713 66-67 0.0034994006895949425 68-69 0.002966883193352234 70-71 1.5214785606934534E-5 72-73 0.0 74-75 0.0 76-77 0.20532353176558155 78-79 3.499400689594943E-4 80-81 0.003271178905490925 82-83 5.173027106357742E-4 84-85 0.0 86-87 0.10120875385732853 88-89 0.3762920776307049 90-91 0.3651396397808219 92-93 0.0 94-95 0.0 96-97 1.5214785606934534E-5 98-99 0.0013236863478033045 100-101 7.303097091328576E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3286277.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.586131948444866 #Duplication Level Percentage of deduplicated Percentage of total 1 71.14637294389433 34.56727063505318 2 15.209984149044061 14.779885935984193 3 5.703490715911763 8.313316574700577 4 2.576898478504243 5.008061179774158 5 1.4196449183461413 3.4487527661352426 6 0.8790382978178409 2.5625442435308394 7 0.5747083545293101 1.9545999161524288 8 0.4099249981615034 1.593333603971266 9 0.29261050108650966 1.2795131173760754 >10 1.6037769499314756 14.211885655157994 >50 0.11915889707100559 3.9860213658322734 >100 0.05913523643811792 5.044304728067132 >500 0.003816537085143294 1.3108131838885368 >1k 0.0012513236335945138 0.9189158812376552 >5k 6.256618167972569E-5 0.24337029161363818 >10k+ 1.2513236335945138E-4 0.7774109215247543 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14245 0.4334692419415649 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11329 0.3447366122819227 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8006 0.24361914713823574 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.0429571213869068E-5 2 3.0429571213869068E-5 0.0 0.0 0.0 3.0429571213869068E-5 3 3.0429571213869068E-5 0.0 0.0 6.0859142427738136E-5 3.0429571213869068E-5 4 3.0429571213869068E-5 0.0 0.0 9.12887136416072E-5 3.0429571213869068E-5 5 3.0429571213869068E-5 0.0 0.0 9.12887136416072E-5 3.0429571213869068E-5 6 3.0429571213869068E-5 0.0 0.0 9.12887136416072E-5 3.0429571213869068E-5 7 3.0429571213869068E-5 0.0 0.0 9.12887136416072E-5 3.0429571213869068E-5 8 3.0429571213869068E-5 0.0 0.0 9.12887136416072E-5 3.0429571213869068E-5 9 3.0429571213869068E-5 0.0 0.0 1.5214785606934534E-4 3.0429571213869068E-5 10-11 3.0429571213869068E-5 0.0 0.0 3.651548545664288E-4 4.56443568208036E-5 12-13 3.0429571213869068E-5 0.0 0.0 4.56443568208036E-4 1.5214785606934534E-4 14-15 3.0429571213869068E-5 0.0 0.0 4.56443568208036E-4 1.9779221289014894E-4 16-17 3.0429571213869068E-5 0.0 0.0 5.781618530635123E-4 2.738661409248216E-4 18-19 4.56443568208036E-5 0.0 0.0 6.84665352312054E-4 2.738661409248216E-4 20-21 6.0859142427738136E-5 0.0 0.0 8.215984227744648E-4 3.195104977456252E-4 22-23 6.0859142427738136E-5 0.0 0.0 8.672427795952684E-4 3.3472528335255975E-4 24-25 9.12887136416072E-5 0.0 0.0 8.82457565202203E-4 3.3472528335255975E-4 26-27 9.12887136416072E-5 0.0 0.0 0.0010650349924854175 3.3472528335255975E-4 28-29 1.2171828485547627E-4 0.0 0.0 0.0020996404137569656 3.8036964017336335E-4 30-31 1.2171828485547627E-4 0.0 0.0 0.0056294706745657775 4.107992113872324E-4 32-33 1.369330704624108E-4 0.0 0.0 0.012339191127223908 4.868731394219051E-4 34-35 1.5214785606934534E-4 0.0 0.0 0.021848432131557988 5.173027106357742E-4 36-37 1.5214785606934534E-4 0.0 0.0 0.036880640311209315 5.173027106357742E-4 38-39 1.5214785606934534E-4 0.0 0.0 0.062517554058894 5.173027106357742E-4 40-41 1.6736264167627987E-4 0.0 0.0 0.08929557672709879 5.173027106357742E-4 42-43 2.28221784104018E-4 0.0 0.0 0.11858403902044776 5.173027106357742E-4 44-45 2.738661409248216E-4 0.0 0.0 0.1555103236884779 5.325174962427087E-4 46-47 3.042957121386907E-4 0.0 0.0 0.2011698952948884 5.477322818496432E-4 48-49 3.3472528335255975E-4 0.0 0.0 0.25335660992667386 6.238062098843158E-4 50-51 3.3472528335255975E-4 0.0 0.0 0.3091187991760889 6.390209954912504E-4 52-53 3.3472528335255975E-4 0.0 0.0 0.36920198753787337 6.694505667051195E-4 54-55 3.3472528335255975E-4 0.0 0.0 0.4371816496296569 6.998801379189886E-4 56-57 3.3472528335255975E-4 0.0 0.0 0.5193719214783172 7.150949235259232E-4 58-59 3.3472528335255975E-4 0.0 0.0 0.6117256701124099 7.303097091328576E-4 60-61 3.3472528335255975E-4 0.0 0.0 0.7156122262365588 7.607392803467267E-4 62-63 3.3472528335255975E-4 0.0 0.0 0.8250826086784528 7.911688515605958E-4 64-65 3.3472528335255975E-4 0.0 0.0 0.9433927815579758 8.368132083813994E-4 66-67 3.3472528335255975E-4 0.0 0.0 1.084403414563045 8.672427795952684E-4 68-69 3.3472528335255975E-4 0.0 0.0 1.24523891321395 8.82457565202203E-4 70-71 3.3472528335255975E-4 0.0 0.0 1.4244843024492457 8.82457565202203E-4 72-73 3.3472528335255975E-4 0.0 0.0 1.6111545070607254 9.281019220230065E-4 74-75 3.3472528335255975E-4 0.0 0.0 1.8112745821487355 9.889610644507447E-4 76-77 3.3472528335255975E-4 0.0 0.0 2.0342320504327542 0.0010041758500576794 78-79 3.3472528335255975E-4 0.0 0.0 2.289642656416364 0.0010041758500576794 80-81 3.3472528335255975E-4 0.0 0.0 2.5732006157728033 0.0010041758500576794 82-83 3.651548545664288E-4 0.0 0.0 2.8688391149011476 0.0010346054212715483 84-85 3.8036964017336335E-4 0.0 0.0 3.1745041577444626 0.001049820206878483 86-87 3.955844257802979E-4 0.0 0.0 3.4985638763865614 0.001080249778092352 88-89 3.955844257802979E-4 0.0 0.0 3.8577241054238582 0.0010954645636992865 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8365 0.0 44.277206 1 GTATCAA 13575 0.0 36.92794 1 TATCAAC 17425 0.0 28.78584 2 ATCAACG 17380 0.0 28.696548 3 TCAACGC 17610 0.0 28.294802 4 CAACGCA 17760 0.0 28.082546 5 AACGCAG 18390 0.0 27.197914 6 ACGCAGA 20695 0.0 24.053968 7 CGCAGAG 21015 0.0 23.552208 8 GAGTACT 13285 0.0 22.021566 12-13 GCAGAGT 22850 0.0 21.349298 9 GTACTTT 13895 0.0 20.884045 14-15 GTACATG 11510 0.0 20.68051 1 GTAAGGT 1325 0.0 20.414354 4 AGTACTT 13740 0.0 20.308006 12-13 AGAGTAC 19415 0.0 20.17699 10-11 TAAGGTG 1435 0.0 20.172264 5 TACATGG 11670 0.0 20.128485 2 CAGAGTA 22135 0.0 18.994633 10-11 ACATGGG 12060 0.0 18.729939 3 >>END_MODULE