FastQCFastQC Report
Fri 27 May 2016
SRR939072_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939072_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3270799
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136280.4166566028667613No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98840.3021891592849331No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69860.21358695535861422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69800.041.9060021
GTATCAA115750.038.643891
ATCAACG146600.030.1172053
TATCAAC148550.029.8813112
TCAACGC149550.029.5231174
CAACGCA150950.029.2174725
AACGCAG155950.028.2980966
ACGCAGA178450.024.6505077
CGCAGAG180400.024.3578038
GTACATG111600.023.4869061
TACATGG111750.022.5950032
GCAGAGT200500.021.7034059
ACATGGG115200.021.3426133
AGAGTAC169700.020.57849110-11
GAGTACT115200.020.38805612-13
AGTACTT118250.019.88221412-13
GTACTTT121550.019.18718314-15
TAGCCCT19250.018.7031864
CATGGGG71150.018.5098384
CAGAGTA193000.018.38855710-11
Produced by FastQC (version 0.11.5)