FastQCFastQC Report
Fri 27 May 2016
SRR939072_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939072_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3270799
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136750.4180935606254007No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110820.3388162953455715No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74920.22905718144098736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83350.044.009741
GTATCAA132050.036.5455631
ATCAACG168850.028.2180333
TATCAAC169750.028.0963762
TCAACGC172100.027.7127274
CAACGCA173250.027.4465965
AACGCAG178650.026.5904146
ACGCAGA202150.023.4288437
CGCAGAG204300.023.0893698
GAGTACT130350.021.86464712-13
GCAGAGT224400.020.9587219
GTACTTT136700.020.65803114-15
AGAGTAC188600.020.5219910-11
GTACATG114700.020.517631
TACATGG113650.020.1266562
AGTACTT134750.020.02360212-13
CAGAGTA216200.018.98880210-11
ACATGGG117900.018.3143543
ACTTTTT158200.017.78929916-17
TACTTTT151600.017.57887514-15
Produced by FastQC (version 0.11.5)