FastQCFastQC Report
Fri 27 May 2016
SRR939071_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939071_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2037320
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT101940.500363222272397No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79940.3923782223705653No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT61950.3040759429053855No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56340.2765397679304184No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT44660.2192095498007186No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC44430.2180806157108358No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38720.190053599827224No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT32110.15760901576581No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT26070.1279622248836707No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG23410.11490585671372194No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC23050.11313882944260106No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC22870.11225531580704062No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACG1401.815572E-523.6899625
GTACATG70500.023.4341811
TACATGG72700.022.4842832
ACATGGG74250.021.0577453
GAGTACT67200.020.33443812-13
AGTACTT68700.019.71809412-13
CATGGGG42000.019.6288244
GTACTTT71650.018.90764814-15
AGAGTAC107900.018.85373310-11
GTAAGCG2002.1827873E-1117.83232394-95
GTATAAG7300.016.9086971
CCGTAAG2002.7730115E-416.6160471
TACTTTT82600.016.5444914-15
ACTTTTT85300.016.24572816-17
TAGACCG2053.398517E-416.178515
GTGTAGC10050.016.0609971
ATGGGGA27550.015.993955
GTATCAA194500.015.4261351
TACCTGG15750.015.3420712
TACACTG16100.015.3028335
Produced by FastQC (version 0.11.5)