##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939071_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2037320 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30922142815071 33.0 31.0 34.0 31.0 34.0 2 32.422861897001944 34.0 31.0 34.0 31.0 34.0 3 32.48902921485088 34.0 31.0 34.0 31.0 34.0 4 35.91659729448491 37.0 35.0 37.0 35.0 37.0 5 35.95496043822276 37.0 37.0 37.0 35.0 37.0 6 36.04276942257476 37.0 37.0 37.0 35.0 37.0 7 35.9762379007716 37.0 37.0 37.0 35.0 37.0 8 35.96751762118862 37.0 37.0 37.0 35.0 37.0 9 37.69227416409793 39.0 38.0 39.0 35.0 39.0 10-11 37.60967005674121 39.0 38.0 39.0 35.0 39.0 12-13 37.52747236565684 39.0 38.0 39.0 35.0 39.0 14-15 38.883755865548856 40.5 39.0 41.0 36.0 41.0 16-17 38.83200479060727 40.0 38.0 41.0 36.0 41.0 18-19 38.75827214183339 40.0 38.5 41.0 35.0 41.0 20-21 38.66103582156951 40.0 38.0 41.0 35.0 41.0 22-23 38.5843289223097 40.0 38.0 41.0 34.5 41.0 24-25 38.6233220603538 40.0 38.0 41.0 35.0 41.0 26-27 38.53937697563466 40.0 38.0 41.0 34.0 41.0 28-29 38.42775018161113 40.0 38.0 41.0 34.0 41.0 30-31 38.27378639585338 40.0 38.0 41.0 34.0 41.0 32-33 38.084339720809695 40.0 38.0 41.0 34.0 41.0 34-35 37.77650418196454 40.0 38.0 41.0 33.0 41.0 36-37 37.52416385251212 40.0 37.5 41.0 33.0 41.0 38-39 37.294333487130146 40.0 37.0 41.0 32.5 41.0 40-41 37.16356340682858 40.0 37.0 41.0 31.5 41.0 42-43 36.9742676653643 40.0 37.0 41.0 31.0 41.0 44-45 36.7221695659003 40.0 36.0 41.0 30.5 41.0 46-47 36.375595635442636 39.5 36.0 41.0 30.0 41.0 48-49 36.355838307187874 40.0 36.0 41.0 30.0 41.0 50-51 35.36202756562543 38.5 35.0 40.0 28.5 40.5 52-53 35.53970804782754 38.5 35.0 40.0 28.5 40.5 54-55 36.04729792079792 39.0 36.0 41.0 29.0 41.0 56-57 35.92376038128522 39.0 35.0 41.0 29.0 41.0 58-59 35.731978039777744 39.0 35.0 41.0 28.0 41.0 60-61 35.4246706457503 39.0 35.0 41.0 28.0 41.0 62-63 35.07887396187148 38.0 35.0 40.0 27.5 41.0 64-65 34.71357150570357 37.5 34.5 40.0 26.5 41.0 66-67 34.31346327528321 37.0 34.0 40.0 26.0 41.0 68-69 33.907765839436124 36.5 34.0 39.0 26.0 41.0 70-71 33.48022819193843 36.0 34.0 39.0 25.0 41.0 72-73 33.033682239412556 35.5 33.5 38.5 24.0 40.0 74-75 32.58749975457955 35.0 33.0 37.5 22.5 39.5 76-77 32.158006596901814 35.0 33.0 37.0 21.5 39.0 78-79 31.736333271160152 35.0 32.0 36.5 20.0 39.0 80-81 31.302156264111677 35.0 32.0 36.0 18.5 37.5 82-83 30.88683785561424 35.0 31.5 35.5 17.0 37.0 84-85 30.520541692026782 35.0 31.0 35.0 12.0 37.0 86-87 30.171921691241437 34.0 31.0 35.0 7.0 36.0 88-89 29.790832073508334 34.0 31.0 35.0 2.0 36.0 90-91 29.428884514951015 34.0 30.0 35.0 2.0 36.0 92-93 29.028746834076138 34.0 30.0 35.0 2.0 35.0 94-95 28.587971207272297 34.0 29.0 35.0 2.0 35.0 96-97 28.05365308346259 34.0 29.0 35.0 2.0 35.0 98-99 27.396154016060315 34.0 28.0 35.0 2.0 35.0 100-101 24.843184919403924 31.5 21.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09994711411849977 1101 2 0.18371101518755495 1101 3 0.19376060107844495 1101 4 0.11566416447047345 1101 5 0.17060545321646003 1101 6 0.229341777684418 1101 7 0.15917800165896523 1101 8 0.25204957537746964 1101 9 0.28178212097623856 1101 10-11 0.22940287592311392 1101 12-13 0.14450154647299485 1101 14-15 0.1782581733203088 1101 16-17 0.23163637432677575 1101 18-19 0.39608930196502 1101 20-21 0.395239369718702 1101 22-23 0.3887839342194752 1101 24-25 0.3489939251928149 1101 26-27 0.2868574210256938 1101 28-29 0.2446856579934007 1101 30-31 0.3116482756060819 1101 32-33 0.25155779590860305 1101 34-35 0.14694484502916794 1101 36-37 0.0673748178221274 1101 38-39 0.17586208655708901 1101 40-41 -0.04410628905515068 1101 42-43 -0.007112898757029029 1101 44-45 0.017179551009554928 1101 46-47 0.08631977930866697 1101 48-49 0.18322386128625823 1101 50-51 0.06797519304075905 1101 52-53 0.08076387905386895 1101 54-55 0.08003536380959986 1101 56-57 0.1559968308378643 1101 58-59 0.18000724476878815 1101 60-61 0.09455604113212246 1101 62-63 0.19084903332158376 1101 64-65 0.12263434052731981 1101 66-67 0.13452459617019485 1101 68-69 -0.05077737506325519 1101 70-71 0.07113312237079583 1101 72-73 -0.03260545838188733 1101 74-75 0.017349578453170977 1101 76-77 -0.017388853513175206 1101 78-79 -0.10589153527768858 1101 80-81 -0.12922753528603081 1101 82-83 -0.09754371640894632 1101 84-85 -0.13480355013000178 1101 86-87 -0.20389138280710384 1101 88-89 -0.31012233698298175 1101 90-91 -0.3553045991614603 1101 92-93 -0.18332859195194473 1101 94-95 -0.28139755093150143 1101 96-97 -0.5216893027993699 1101 98-99 -0.5765871351303851 1101 100-101 -0.5958474138614491 1102 1 0.19231208664150756 1102 2 0.19091356453233743 1102 3 0.2188963601913798 1102 4 0.2413348307469363 1102 5 0.2088537541952249 1102 6 0.23712963577956003 1102 7 0.2685575743835784 1102 8 0.2496514353586079 1102 9 0.27909132658218994 1102 10-11 0.15301195471217 1102 12-13 0.15608984680142157 1102 14-15 0.30581076981496125 1102 16-17 0.3406582583706026 1102 18-19 0.4735329659977978 1102 20-21 0.4541807738093624 1102 22-23 0.363501847918954 1102 24-25 0.3069695698453714 1102 26-27 0.36059006499538526 1102 28-29 0.38354609437359244 1102 30-31 0.4240356849645224 1102 32-33 0.40207563156692316 1102 34-35 0.3210823275512027 1102 36-37 0.48185877060721793 1102 38-39 0.5510124783887846 1102 40-41 0.4526501249532373 1102 42-43 0.5017114435835595 1102 44-45 0.4321024853574258 1102 46-47 0.46935964533800245 1102 48-49 0.5233532795543923 1102 50-51 0.47752873253283923 1102 52-53 0.39413168056916703 1102 54-55 0.30811067353548083 1102 56-57 0.3777882007855098 1102 58-59 0.27328269757013857 1102 60-61 0.1750481882397139 1102 62-63 0.1415855468324665 1102 64-65 0.3492667015977915 1102 66-67 0.2511067182395834 1102 68-69 0.31020773208076236 1102 70-71 0.2768244370392807 1102 72-73 0.38092330303976496 1102 74-75 0.2174316842639854 1102 76-77 0.09814865502257675 1102 78-79 0.12149278495123284 1102 80-81 0.14792835194448273 1102 82-83 0.08962907974101952 1102 84-85 0.1528106663691986 1102 86-87 0.03340660438113474 1102 88-89 0.14331310021013977 1102 90-91 0.14594731122126703 1102 92-93 0.007465802090916185 1102 94-95 0.17406207902330095 1102 96-97 0.0508686590254932 1102 98-99 -0.09166290009640576 1102 100-101 -0.004136849038506085 1103 1 0.2418813375682234 1103 2 0.23800531976888806 1103 3 0.37293822916537067 1103 4 0.2526467549547817 1103 5 0.261518409801063 1103 6 0.2577107807950938 1103 7 0.22279334196775835 1103 8 0.20478901606179534 1103 9 0.28076574567742796 1103 10-11 0.2592240349061541 1103 12-13 0.25306859706071094 1103 14-15 0.34970296359402653 1103 16-17 0.3551836609050625 1103 18-19 0.36009480787638637 1103 20-21 0.40291615523705104 1103 22-23 0.32458383615026065 1103 24-25 0.34534130787773876 1103 26-27 0.28372265554879306 1103 28-29 0.37978194750989047 1103 30-31 0.33267041373499495 1103 32-33 0.2709935202811877 1103 34-35 0.32213502883013945 1103 36-37 0.1550190484055065 1103 38-39 0.2086815229356489 1103 40-41 0.315859020967558 1103 42-43 0.23515344569071317 1103 44-45 0.22356142444079552 1103 46-47 0.13387740091910416 1103 48-49 0.19434853199761193 1103 50-51 0.24611786085048237 1103 52-53 0.29094586018877777 1103 54-55 0.3299668967491627 1103 56-57 0.19194701833799144 1103 58-59 0.2694357819585136 1103 60-61 0.28995538176681634 1103 62-63 0.291592402721669 1103 64-65 0.09497810850828614 1103 66-67 0.12399999724145516 1103 68-69 -0.015783793571394256 1103 70-71 0.16477093414867738 1103 72-73 0.011513050145346426 1103 74-75 -0.05006943787514473 1103 76-77 0.09041190124509768 1103 78-79 0.06277502374478061 1103 80-81 0.08001300518298393 1103 82-83 0.09854862090038097 1103 84-85 0.0780744423117099 1103 86-87 -0.16605831397518855 1103 88-89 -0.11162108911318924 1103 90-91 -0.13775582994793822 1103 92-93 -0.26668831595460674 1103 94-95 -0.4214331329877474 1103 96-97 -0.3707754125054592 1103 98-99 -0.3342915007566809 1103 100-101 -0.5887243120196359 1104 1 0.18837137890713507 1104 2 0.2136094509996127 1104 3 0.22835703849507638 1104 4 0.15652537946627376 1104 5 0.1533162489950044 1104 6 0.24026122917324244 1104 7 0.18009335591811748 1104 8 0.1806042419882985 1104 9 0.24559244570422578 1104 10-11 0.24085214660750864 1104 12-13 0.27900975823434493 1104 14-15 0.37111308748058747 1104 16-17 0.33002717210595733 1104 18-19 0.2830428386826256 1104 20-21 0.2104518397200934 1104 22-23 0.3442177651234104 1104 24-25 0.38606178595912155 1104 26-27 0.4200490815203466 1104 28-29 0.30191078375276703 1104 30-31 0.25822769948732827 1104 32-33 0.16982242576650464 1104 34-35 0.21498720962794238 1104 36-37 0.2973865744577395 1104 38-39 0.38782465957079637 1104 40-41 0.3115080932969221 1104 42-43 0.26521038749451264 1104 44-45 0.2194213084971821 1104 46-47 0.24391772679724255 1104 48-49 0.35698114884461063 1104 50-51 0.16655892991777677 1104 52-53 0.26332046894909666 1104 54-55 0.282300384489119 1104 56-57 0.33142543333941177 1104 58-59 0.2959860333904132 1104 60-61 0.2849059457094114 1104 62-63 0.25314190004181825 1104 64-65 0.16627463458569736 1104 66-67 0.1592799764375954 1104 68-69 0.15629134934904698 1104 70-71 0.09595887379370538 1104 72-73 0.11605840565173509 1104 74-75 0.15294621680562415 1104 76-77 -0.016810763194420986 1104 78-79 0.0804809450641848 1104 80-81 -0.03823341476699227 1104 82-83 -0.1348684900508239 1104 84-85 -0.005281221973021388 1104 86-87 -0.102307857334349 1104 88-89 0.014720076701127738 1104 90-91 -0.07808281167013575 1104 92-93 -0.17599399833518703 1104 94-95 -0.28571112828990053 1104 96-97 -0.15486656919403075 1104 98-99 -0.3675251913569362 1104 100-101 -0.6042929702864654 1105 1 0.0679324758721691 1105 2 0.13372687966834462 1105 3 0.16767295605765753 1105 4 0.11327289126903395 1105 5 0.15509335531724133 1105 6 0.16708137629854747 1105 7 0.11824735119204632 1105 8 0.20430386879321105 1105 9 0.1701422905144625 1105 10-11 0.19422911780452523 1105 12-13 0.26083561009449596 1105 14-15 0.28853560161545744 1105 16-17 0.16689783663166935 1105 18-19 0.23302912192782088 1105 20-21 0.1842194431187565 1105 22-23 0.30971515893998713 1105 24-25 0.2941339995673573 1105 26-27 0.19132467899955685 1105 28-29 0.1824345374738101 1105 30-31 0.12851894731458202 1105 32-33 0.16149177414613547 1105 34-35 0.09089363320905619 1105 36-37 0.027078601573307992 1105 38-39 0.0449554677332884 1105 40-41 0.09140813486117594 1105 42-43 0.047044436288629754 1105 44-45 -0.04859800866785946 1105 46-47 -0.022161511334999773 1105 48-49 0.038434081608222925 1105 50-51 0.10899009637184065 1105 52-53 0.10613791246615278 1105 54-55 0.05968807959408906 1105 56-57 0.08961325959532473 1105 58-59 0.11379551456588644 1105 60-61 0.1565339561507102 1105 62-63 0.22544868285287123 1105 64-65 0.10517311569659427 1105 66-67 0.18266386948995716 1105 68-69 0.1739041317085892 1105 70-71 0.04429735252003297 1105 72-73 0.1552547914938387 1105 74-75 0.1730085576622855 1105 76-77 0.16481949381395822 1105 78-79 0.13416575666994035 1105 80-81 -0.03661072543300037 1105 82-83 0.0931693847519135 1105 84-85 0.1113643221791989 1105 86-87 0.208041171977559 1105 88-89 0.17887389092493322 1105 90-91 0.11509987839247415 1105 92-93 0.1320683071946256 1105 94-95 -0.1660946785613966 1105 96-97 -0.19210645453631514 1105 98-99 -0.368654546702583 1105 100-101 -0.34756872452858545 1106 1 -0.0012921165573871463 1106 2 0.01257651155396644 1106 3 -0.03845990209673289 1106 4 0.06207118762030461 1106 5 0.06682641480418994 1106 6 0.00914196374597509 1106 7 0.15429472832374103 1106 8 0.017442630427417782 1106 9 0.08023312476488087 1106 10-11 0.050318201058949796 1106 12-13 0.153006194641371 1106 14-15 0.06996368707942224 1106 16-17 0.11082570881962539 1106 18-19 0.12228555646488104 1106 20-21 0.17600778374612247 1106 22-23 0.1522409546727701 1106 24-25 0.15899245894680547 1106 26-27 0.16404667799289285 1106 28-29 0.08144781603786555 1106 30-31 0.09561925741789423 1106 32-33 -0.03620815078071615 1106 34-35 -0.03923005620989528 1106 36-37 -0.0031082175430512393 1106 38-39 0.18735905631534422 1106 40-41 0.0888754823048501 1106 42-43 0.08647783659062469 1106 44-45 0.051016548272563966 1106 46-47 0.020508085743969673 1106 48-49 -0.09800844264125175 1106 50-51 -0.21303001128988797 1106 52-53 -0.13520868023639565 1106 54-55 -0.299054956944353 1106 56-57 -0.1778121897772209 1106 58-59 -0.19736697828936656 1106 60-61 -0.1296123277281893 1106 62-63 -0.004681191570220733 1106 64-65 -0.18700647976147877 1106 66-67 -0.2538633253333984 1106 68-69 -0.256443657472353 1106 70-71 -0.21362238532110212 1106 72-73 -0.30167971784338476 1106 74-75 -0.4773382291214219 1106 76-77 -0.4301189867224693 1106 78-79 -0.3262611472449528 1106 80-81 -0.20152399674567079 1106 82-83 -0.10525541199605115 1106 84-85 -0.09767255537477837 1106 86-87 -0.15203430640051963 1106 88-89 -0.11904309565574067 1106 90-91 -0.15152631915229264 1106 92-93 -0.3472671141107959 1106 94-95 -0.30108106088848885 1106 96-97 -0.21864785354159366 1106 98-99 -0.21510861984771878 1106 100-101 -0.23608162559411383 1107 1 0.1697271970120795 1107 2 0.2118421517824416 1107 3 0.20878663429123634 1107 4 0.16933444063978698 1107 5 0.21081999339703117 1107 6 0.13277101490750454 1107 7 0.055989677557001016 1107 8 0.10240830273209411 1107 9 0.2010393167038842 1107 10-11 0.2611732937714919 1107 12-13 0.27272403208909424 1107 14-15 0.3917661686664573 1107 16-17 0.43419884439088463 1107 18-19 0.3283350031505492 1107 20-21 0.2499642279767258 1107 22-23 0.30204178927063907 1107 24-25 0.3644339229782929 1107 26-27 0.35746920781758007 1107 28-29 0.30162235370888624 1107 30-31 0.3819413054828331 1107 32-33 0.40657054199103726 1107 34-35 0.4027262985774769 1107 36-37 0.44814714905082553 1107 38-39 0.626149429139403 1107 40-41 0.5891206801286017 1107 42-43 0.6005015328784253 1107 44-45 0.523598728096637 1107 46-47 0.586945130046125 1107 48-49 0.7194036099883689 1107 50-51 0.6458517015788345 1107 52-53 0.6117436958693006 1107 54-55 0.6804563745877701 1107 56-57 0.6338084136253244 1107 58-59 0.5949855483277062 1107 60-61 0.5087852659404604 1107 62-63 0.5644187165191497 1107 64-65 0.4663331454405366 1107 66-67 0.38920382185931146 1107 68-69 0.449899563964415 1107 70-71 0.49171190104528506 1107 72-73 0.5270176031776259 1107 74-75 0.6199821750386079 1107 76-77 0.6195809929214846 1107 78-79 0.640679940508111 1107 80-81 0.434508748729737 1107 82-83 0.567119814696273 1107 84-85 0.5657159397582738 1107 86-87 0.545028408725873 1107 88-89 0.4428095975107986 1107 90-91 0.3365526715579463 1107 92-93 0.42194968702914437 1107 94-95 0.39791663517724274 1107 96-97 0.5921824109636304 1107 98-99 0.5921011214454452 1107 100-101 0.41897939627938285 1108 1 0.006396874313033152 1108 2 0.04912988121059669 1108 3 0.05030745203107756 1108 4 0.005208612810150726 1108 5 0.0517386842792078 1108 6 0.13150761019105062 1108 7 0.14182763869782633 1108 8 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-0.9457729257650342 2316 70-71 -0.8043236048332929 2316 72-73 -0.8016289048352547 2316 74-75 -0.9076227819669533 2316 76-77 -0.9271210192427937 2316 78-79 -0.8711290334238164 2316 80-81 -0.7471032650383513 2316 82-83 -0.8495135420773572 2316 84-85 -0.8284144252934809 2316 86-87 -0.6537619486769444 2316 88-89 -0.5527321200459845 2316 90-91 -0.7636706280953831 2316 92-93 -0.8828870328099825 2316 94-95 -0.8114977275551567 2316 96-97 -0.8635157397204551 2316 98-99 -0.7506777255387753 2316 100-101 -0.6282056093339534 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5760.0 3 1442.0 4 1495.0 5 1519.0 6 1839.0 7 2090.0 8 2445.0 9 3057.0 10 3743.0 11 4610.0 12 6274.0 13 10248.0 14 9105.0 15 8956.0 16 11207.0 17 14888.0 18 14174.0 19 9545.0 20 9260.0 21 9920.0 22 10391.0 23 11077.0 24 12516.0 25 14388.0 26 16762.0 27 19498.0 28 23120.0 29 28537.0 30 34612.0 31 44116.0 32 56646.0 33 76915.0 34 109130.0 35 169039.0 36 275674.0 37 453573.0 38 461579.0 39 88112.0 40 58.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.71397621802605 18.630246928777172 14.522661808513257 23.13311504468352 2 15.105429915836893 19.90485952505435 40.762008278724934 24.227702280383827 3 20.439525991554795 23.52678163438379 28.92207201319944 27.11162036086197 4 13.092640193173002 16.12305639077239 36.76301566484052 34.02128775121408 5 14.488898150191352 36.561525218564825 33.53609360555665 15.413483025687173 6 32.59913149414446 35.03841468207184 17.25742749351568 15.105026330268018 7 29.196216797247292 32.03450315496158 20.28268888011146 18.486591167679673 8 25.803625865971387 34.695059670408874 19.47130607718955 20.030008386430197 9 25.76974449842138 18.22136789211075 20.887699856998502 35.12118775246937 10-11 25.441262762730677 26.1204516365303 26.679753308933424 21.758532291805597 12-13 26.643646381698527 23.365638368320628 27.297730001434466 22.69298524854637 14-15 22.927539442893007 24.973625317140975 26.355965738335147 25.74286950163087 16-17 22.535366934633156 28.3324520100434 26.655661791921414 22.47651926340203 18-19 22.15253911292373 27.2314527573165 28.7228421537968 21.893165975962972 20-21 23.02170698033204 25.95104383159157 28.629335377268106 22.397913810808287 22-23 22.519604873265756 25.917152320142378 28.783877508452104 22.779365298139766 24-25 22.98590088850448 26.038095612517694 28.688153601195154 22.287849897782667 26-27 22.600343508355508 26.05643562166904 29.110047987969836 22.23317288200562 28-29 21.99016362911324 26.940437548234065 28.88198689089943 22.187411931753267 30-31 22.351326630713974 26.094985958206536 28.69626167191069 22.857425739168804 32-33 22.190075782291 26.28496774928954 28.87848694838707 22.64646952003239 34-35 23.160128026201992 26.245592975447202 28.338955795214204 22.255323203136598 36-37 22.745096298838536 25.915576160847515 28.813639138483143 22.525688401830806 38-39 22.32042412177824 26.538496426377662 28.821665941983976 22.319413509860123 40-41 22.5728140984379 26.484375539076183 28.616455734883438 22.326354627602473 42-43 22.313324496467512 26.58659910745519 28.029915510720965 23.070160885356334 44-45 22.59196658199088 26.305464941068806 28.09847307467773 23.00409540226258 46-47 22.95552515855695 26.761853432335066 27.811026549597678 22.47159485951031 48-49 22.982360277607775 26.703276977028267 27.790514919716554 22.5238478256474 50-51 22.184617827359585 26.98995652825663 28.225296649283226 22.60012899510055 52-53 22.835609186849286 27.17766796084955 27.393430856880236 22.593291995420923 54-55 22.260066705009677 26.551492998537856 27.889537608406734 23.29890268804573 56-57 22.696728244988257 26.900414860034033 27.62733186239424 22.77552503258347 58-59 23.335833683661654 26.495441953348585 27.50313077702904 22.665593585960714 60-61 22.840665731611608 26.58455622080108 28.3990950952963 22.17568295229101 62-63 22.43177668645783 26.833110624199353 28.16396716574711 22.571145523595707 64-65 22.174683099225344 27.1377872658766 27.80464182057088 22.882887814327177 66-67 22.460537749909502 26.890898456032993 27.38881869213083 23.25974510192668 68-69 22.18894159805364 26.76000586966628 27.835165959881568 23.21588657239851 70-71 22.674360107480638 26.712479433083047 27.63863187092854 22.974528588507777 72-73 22.670300963792318 26.37692895317349 28.14199107470019 22.810779008333995 74-75 22.174069259873843 27.385858860856104 27.497396509559614 22.94267536971044 76-77 22.40641885261826 26.835701317438733 27.39511229145977 23.362767538483233 78-79 22.556649333183465 26.88457260836385 27.604336131537277 22.954441926915408 80-81 22.65910766548092 26.960826484539403 27.485402892971965 22.894662957007707 82-83 22.598521848697438 26.796848247521194 27.751120915379918 22.85350898840145 84-85 22.444912851613537 26.945270320242585 27.547222838547448 23.06259398959643 86-87 22.454421064309752 26.933002970797432 27.645920392490414 22.9666555724024 88-89 22.284685889531524 26.962236521470693 27.221600462165384 23.531477126832396 90-91 22.490178317997344 26.65083294719016 27.46125272160035 23.39773601321214 92-93 22.885687337019846 26.67956329024184 27.526499109195935 22.908250263542378 94-95 22.559706367503736 26.505787597311752 27.62533053753794 23.309175497646564 96-97 22.196696344198873 26.479996764603182 28.049213542108493 23.274093349089455 98-99 22.331354906162385 26.69318268683068 27.297067374198907 23.678395032808027 100-101 22.34433155618147 25.880341071143278 27.99061028696762 23.784717085707634 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2215.0 1 1344.0 2 561.0 3 652.0 4 556.0 5 535.0 6 992.5 7 2057.5 8 3270.5 9 3560.0 10 2932.0 11 2441.0 12 2413.5 13 2607.5 14 2812.5 15 2944.5 16 3094.0 17 3190.5 18 3263.5 19 3349.5 20 3535.5 21 4041.0 22 4842.0 23 5886.5 24 7251.0 25 8850.5 26 10953.5 27 13474.0 28 15820.0 29 18581.0 30 21655.5 31 24709.5 32 28029.5 33 30818.0 34 33730.0 35 36621.5 36 39900.0 37 44127.0 38 47078.0 39 48838.0 40 51483.5 41 56506.5 42 61289.5 43 64710.5 44 68330.5 45 71445.0 46 73241.0 47 81975.0 48 100983.5 49 100836.0 50 85243.0 51 77988.0 52 73193.5 53 71369.5 54 70440.0 55 65444.5 56 58505.0 57 52665.0 58 47293.5 59 41130.0 60 34615.0 61 28160.0 62 22836.0 63 18520.5 64 14873.5 65 11832.5 66 9077.5 67 7491.5 68 5882.0 69 4110.0 70 3060.5 71 2308.5 72 1859.5 73 1557.5 74 1238.0 75 1035.0 76 779.5 77 488.5 78 301.5 79 213.0 80 153.0 81 106.5 82 78.0 83 55.5 84 40.0 85 36.5 86 30.5 87 17.0 88 12.5 89 11.0 90 7.5 91 7.0 92 6.0 93 4.5 94 4.5 95 3.5 96 1.5 97 1.5 98 1.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3126656588066676 2 0.10916301808257907 3 0.10219307717982448 4 0.11058645671764868 5 0.12609702943082088 6 0.08049790901772919 7 0.08815502719258633 8 0.09271984764298195 9 0.08231402037971453 10-11 0.08130779651699292 12-13 0.08462097265034457 14-15 0.085921701058253 16-17 0.07635030333968154 18-19 0.09009384878173286 20-21 0.09964070445487208 22-23 0.10678243967565232 24-25 0.11976518170930438 26-27 0.11951976125498204 28-29 0.1139487169418648 30-31 0.10530991694971827 32-33 0.09895352718276952 34-35 0.3106777531266566 36-37 0.4595252586731589 38-39 0.43419786778709285 40-41 0.4116191859894371 42-43 0.44541358254962404 44-45 0.45118096322619916 46-47 0.45854357685586944 48-49 0.46958749730037497 50-51 0.4582490723106827 52-53 0.4613659120805765 54-55 0.4484567961832211 56-57 0.4262462450670489 58-59 0.4059745155400232 60-61 0.3677870928474663 62-63 0.43633302573969723 64-65 0.4671087507117193 66-67 0.47542850411324683 68-69 0.48826399387430547 70-71 0.48063141774488055 72-73 0.47125635638976693 74-75 0.4787416802465984 76-77 0.45618754049437493 78-79 0.4356703905130269 80-81 0.4379037166473603 82-83 0.4505183279995288 84-85 0.4501011132271808 86-87 0.45378242004201597 88-89 0.4650472188954116 90-91 0.48055779160858386 92-93 0.4740296075236095 94-95 0.47864351206486955 96-97 0.47847171774684394 98-99 0.4719435336618695 100-101 0.45923075412797204 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2037320.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.411334135952 #Duplication Level Percentage of deduplicated Percentage of total 1 78.84057987771844 44.47502294954189 2 12.55577058670767 14.165755398022492 3 3.7379535890641495 6.325888466921363 4 1.6160173112486436 3.6464677005731994 5 0.8319404206746533 2.3465434525941173 6 0.5081083020355867 1.719784032202842 7 0.32349671996884716 1.2774217093432993 8 0.24793051648381556 1.118887296629414 9 0.18503613490890997 0.939432171021445 >10 1.0103086981530434 10.562809911200013 >50 0.08736373892111407 3.4518511393580247 >100 0.049668263922801686 5.074624181371419 >500 0.003912877741096988 1.497387703350475 >1k 0.001565151096438792 1.9257523252707291 >5k 2.60858516073132E-4 0.9723430551156818 >10k+ 8.6952838691044E-5 0.5000285074836938 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10194 0.500363222272397 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7994 0.3923782223705653 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6195 0.3040759429053855 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5634 0.2765397679304184 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4466 0.2192095498007186 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4443 0.2180806157108358 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3872 0.190053599827224 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3211 0.15760901576581 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2607 0.1279622248836707 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2341 0.11490585671372194 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2305 0.11313882944260106 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2287 0.11225531580704062 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.908409086446901E-5 0.0 2 0.0 0.0 0.0 4.908409086446901E-5 0.0 3 0.0 0.0 0.0 4.908409086446901E-5 0.0 4 0.0 0.0 0.0 9.816818172893802E-5 0.0 5 0.0 0.0 0.0 9.816818172893802E-5 0.0 6 0.0 0.0 0.0 9.816818172893802E-5 0.0 7 0.0 0.0 0.0 1.4725227259340703E-4 0.0 8 0.0 0.0 0.0 1.9633636345787604E-4 0.0 9 0.0 0.0 0.0 1.9633636345787604E-4 0.0 10-11 0.0 0.0 0.0 2.4542045432234504E-4 0.0 12-13 0.0 0.0 0.0 2.9450454518681405E-4 0.0 14-15 0.0 0.0 0.0 3.4358863605128307E-4 0.0 16-17 0.0 0.0 0.0 3.4358863605128307E-4 0.0 18-19 0.0 0.0 0.0 3.6813068148351755E-4 0.0 20-21 0.0 0.0 0.0 4.4175681778022105E-4 0.0 22-23 0.0 0.0 0.0 4.662988632124556E-4 0.0 24-25 0.0 0.0 0.0 4.908409086446901E-4 0.0 26-27 0.0 0.0 0.0 7.117193175348006E-4 0.0 28-29 0.0 0.0 0.0 0.0015216068167985394 0.0 30-31 0.0 0.0 0.0 0.004343942041505507 0.0 32-33 0.0 0.0 0.0 0.010332201126970727 0.0 34-35 0.0 0.0 0.0 0.018627412483065986 0.0 36-37 0.0 0.0 0.0 0.03374531246932244 0.0 38-39 0.0 0.0 0.0 0.05853277835587929 0.0 40-41 0.0 0.0 0.0 0.08609349537627864 0.0 42-43 0.0 0.0 0.0 0.11461135216853513 0.0 44-45 0.0 0.0 0.0 0.15233247599787958 0.0 46-47 0.0 0.0 0.0 0.20153927708950975 0.0 48-49 0.0 0.0 0.0 0.25857499067402273 0.0 50-51 0.0 0.0 0.0 0.32147625311683975 0.0 52-53 0.0 0.0 0.0 0.38464747805941135 0.0 54-55 0.0 0.0 0.0 0.4559175779946204 0.0 56-57 0.0 0.0 0.0 0.5474594074568551 0.0 58-59 0.0 0.0 0.0 0.6484990085013645 0.0 60-61 0.0 0.0 0.0 0.759797184536548 0.0 62-63 0.0 0.0 0.0 0.8748993776137278 0.0 64-65 0.0 0.0 0.0 1.0000638093181238 0.0 66-67 0.0 0.0 0.0 1.1437084012329923 0.0 68-69 0.0 0.0 0.0 1.3165580272122201 0.0 70-71 0.0 0.0 0.0 1.5103911020360081 0.0 72-73 0.0 0.0 0.0 1.7049604382227632 0.0 74-75 0.0 0.0 0.0 1.9142059175779946 0.0 76-77 0.0 0.0 0.0 2.1425205662340723 0.0 78-79 0.0 0.0 0.0 2.4173178489388016 0.0 80-81 0.0 0.0 0.0 2.7234798657059276 0.0 82-83 0.0 0.0 0.0 3.028635658610331 0.0 84-85 0.0 0.0 0.0 3.345939764003691 0.0 86-87 0.0 0.0 0.0 3.67531855574971 0.0 88-89 0.0 0.0 0.0 4.05500854063181 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGACG 140 1.815572E-5 23.689962 5 GTACATG 7050 0.0 23.434181 1 TACATGG 7270 0.0 22.484283 2 ACATGGG 7425 0.0 21.057745 3 GAGTACT 6720 0.0 20.334438 12-13 AGTACTT 6870 0.0 19.718094 12-13 CATGGGG 4200 0.0 19.628824 4 GTACTTT 7165 0.0 18.907648 14-15 AGAGTAC 10790 0.0 18.853733 10-11 GTAAGCG 200 2.1827873E-11 17.832323 94-95 GTATAAG 730 0.0 16.908697 1 CCGTAAG 200 2.7730115E-4 16.616047 1 TACTTTT 8260 0.0 16.54449 14-15 ACTTTTT 8530 0.0 16.245728 16-17 TAGACCG 205 3.398517E-4 16.17851 5 GTGTAGC 1005 0.0 16.060997 1 ATGGGGA 2755 0.0 15.99395 5 GTATCAA 19450 0.0 15.426135 1 TACCTGG 1575 0.0 15.342071 2 TACACTG 1610 0.0 15.302833 5 >>END_MODULE