FastQCFastQC Report
Fri 27 May 2016
SRR939071_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939071_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2037320
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT119310.5856222881039796No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81710.40106610645357627No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62990.3091806883552903No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT53400.2621090452162645No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45970.225639565703964No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT36240.17788074529283568No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC36140.177389904384191No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT35680.1751320362044254No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC27180.13341055896962675No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC26780.131447195335048No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG25970.12747138397502603No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT24080.11819449080164136No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA20920.10268391808846916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG70900.022.762741
TACATGG70900.022.4190732
ACATGGG69450.021.725713
AGTACTT79100.020.24642612-13
GAGTACT77550.019.7638612-13
AGAGTAC116000.019.53288710-11
TAGACCG1507.780817E-418.9792525
CATGGGG40800.018.8396994
GTACTTT82500.018.5780314-15
GTAAGCG2250.017.9257394-95
CGCGGAA3850.017.34560282-83
ATTCGCG4100.016.80208680-81
TACTTTT96250.016.5649314-15
CCGTAAG2304.191906E-516.5589681
TCGCGCT4750.016.4852260-61
CGATCGT4750.016.48279224-25
ATGGGGA27300.016.3374525
TAAGGTG8700.015.8160425
CGCGATC4850.015.65337422-23
ACTTTTT98000.015.638916-17
Produced by FastQC (version 0.11.5)