##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939071_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2037320 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.34485451475468 34.0 31.0 34.0 31.0 34.0 2 32.57597726424911 34.0 31.0 34.0 31.0 34.0 3 32.668797734278364 34.0 31.0 34.0 31.0 34.0 4 36.166606620462176 37.0 35.0 37.0 35.0 37.0 5 36.120153436868044 37.0 35.0 37.0 35.0 37.0 6 36.15973337521842 37.0 36.0 37.0 35.0 37.0 7 36.112524296624976 37.0 36.0 37.0 35.0 37.0 8 36.09560206545854 37.0 36.0 37.0 35.0 37.0 9 37.806063848585396 39.0 38.0 39.0 35.0 39.0 10-11 37.736160495160306 39.0 37.5 39.0 35.0 39.0 12-13 37.65598187815365 39.0 37.0 39.0 35.0 39.0 14-15 38.95092597137416 40.0 38.0 41.0 35.5 41.0 16-17 38.88868587163529 40.0 38.0 41.0 35.0 41.0 18-19 38.91288261048829 40.0 38.5 41.0 35.0 41.0 20-21 38.99973151002297 40.0 38.5 41.0 35.0 41.0 22-23 38.95179770482791 40.0 38.0 41.0 35.0 41.0 24-25 38.876880411521014 40.0 38.0 41.0 35.0 41.0 26-27 38.72050340643591 40.0 38.0 41.0 34.5 41.0 28-29 38.55003288634088 40.0 38.0 41.0 34.0 41.0 30-31 38.34819959554709 40.0 38.0 41.0 34.0 41.0 32-33 38.19182651718925 40.0 38.0 41.0 34.0 41.0 34-35 38.11537730940647 40.0 38.0 41.0 33.5 41.0 36-37 37.9876106846249 40.0 37.5 41.0 33.0 41.0 38-39 37.837936602988236 40.0 37.0 41.0 33.0 41.0 40-41 37.66431267547563 40.0 37.0 41.0 33.0 41.0 42-43 37.46954773918678 40.0 37.0 41.0 32.5 41.0 44-45 37.222859933638304 40.0 37.0 41.0 31.5 41.0 46-47 36.9211014960831 40.0 36.0 41.0 31.0 41.0 48-49 36.5763770541692 39.5 36.0 41.0 30.0 41.0 50-51 36.27792737517916 39.0 35.0 41.0 30.0 41.0 52-53 36.02965194471168 39.0 35.0 40.5 29.5 41.0 54-55 35.75628055484656 39.0 35.0 40.0 28.5 41.0 56-57 35.43043581764279 38.0 35.0 40.0 28.0 41.0 58-59 35.097637337286244 38.0 34.0 40.0 27.5 41.0 60-61 34.67839220151964 38.0 34.0 40.0 26.0 41.0 62-63 34.25937187088921 37.0 34.0 40.0 26.0 41.0 64-65 33.808898454832814 36.5 33.0 39.0 25.5 41.0 66-67 33.40209662694127 36.0 33.0 39.0 24.5 40.0 68-69 32.89427483164157 35.5 32.0 38.5 22.5 40.0 70-71 32.37980361455245 35.0 31.5 38.0 22.0 40.0 72-73 31.849699114523 35.0 31.0 37.0 20.5 39.0 74-75 31.305811065517446 35.0 31.0 37.0 18.5 39.0 76-77 29.36819620874482 32.5 28.5 35.0 13.0 37.0 78-79 30.366384514951015 34.0 30.0 36.0 14.5 37.5 80-81 30.3693676005733 34.0 30.0 35.0 13.5 37.0 82-83 30.04410500068718 34.0 30.0 35.0 8.5 37.0 84-85 29.612833035556513 34.0 29.5 35.0 4.5 36.0 86-87 28.993662016767125 34.0 29.0 35.0 2.0 36.0 88-89 28.378246421769774 34.0 28.5 35.0 2.0 35.5 90-91 27.83607877014902 33.0 27.0 35.0 2.0 35.0 92-93 27.45520315905209 33.0 26.0 35.0 2.0 35.0 94-95 27.002882463236016 33.0 25.5 35.0 2.0 35.0 96-97 26.291173944201205 33.0 25.0 35.0 2.0 35.0 98-99 25.327936946576877 32.0 21.0 34.0 2.0 35.0 100-101 21.61228206663656 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15265862981288336 1101 2 0.14436528924024827 1101 3 0.20225641018096496 1101 4 0.1514930775282508 1101 5 0.18641254033980204 1101 6 0.22738867899236226 1101 7 0.16248558626642762 1101 8 0.23166153508435627 1101 9 0.20986056605214998 1101 10-11 0.17361785226968607 1101 12-13 0.17691800664106694 1101 14-15 0.2242585128930017 1101 16-17 0.21903409157897613 1101 18-19 0.27557372976040995 1101 20-21 0.2974224004751207 1101 22-23 0.28101585164368004 1101 24-25 0.14947993484472732 1101 26-27 0.21792009306032156 1101 28-29 0.1535311838069049 1101 30-31 0.2195970287149791 1101 32-33 0.3094852363953535 1101 34-35 0.37932367491694663 1101 36-37 0.36266078172361205 1101 38-39 0.44018557813937065 1101 40-41 0.46651593117005774 1101 42-43 0.254207985603351 1101 44-45 0.3348693796080653 1101 46-47 0.2540239910625317 1101 48-49 0.2140391697077817 1101 50-51 0.23977567717253123 1101 52-53 0.22211105872549552 1101 54-55 0.2564034615302049 1101 56-57 0.20427684023501058 1101 58-59 0.1866510917580939 1101 60-61 0.30443440316985715 1101 62-63 0.23554008158246376 1101 64-65 0.0024342385239535247 1101 66-67 0.12619236750152396 1101 68-69 -0.053752498804293225 1101 70-71 0.0031946003648002375 1101 72-73 0.017831563320442 1101 74-75 -0.08178245524047512 1101 76-77 -0.1304293026958021 1101 78-79 0.19498087839499334 1101 80-81 0.1462733701088723 1101 82-83 0.27818883594774846 1101 84-85 0.23822357755144807 1101 86-87 0.23360762789631906 1101 88-89 0.4797030524519599 1101 90-91 0.5828559750401965 1101 92-93 0.1523444157132694 1101 94-95 -0.19790752560911073 1101 96-97 -0.3693431255073314 1101 98-99 -0.6407983178027798 1101 100-101 -0.7912585213617582 1102 1 0.22253799117599726 1102 2 0.167444338438699 1102 3 0.18453456190599837 1102 4 0.11348865519546791 1102 5 0.05876645703086325 1102 6 0.1430473139222883 1102 7 0.08642796587167112 1102 8 0.07803424668973236 1102 9 0.25024686982739297 1102 10-11 0.23088868763534265 1102 12-13 0.22800651833098584 1102 14-15 0.2511827154098327 1102 16-17 0.2052996591658811 1102 18-19 0.2309073557481156 1102 20-21 0.25038227430857063 1102 22-23 0.24516162603967473 1102 24-25 0.15188213950768414 1102 26-27 0.1768184119235272 1102 28-29 0.10941760139218104 1102 30-31 0.16760210139081266 1102 32-33 0.12423095226046854 1102 34-35 0.23445569455730464 1102 36-37 0.30369092512425055 1102 38-39 0.1933397419607843 1102 40-41 0.20855735802272335 1102 42-43 0.10925871236172213 1102 44-45 0.24885744581298752 1102 46-47 0.16013477700212064 1102 48-49 0.21025905069500794 1102 50-51 0.14562226124563438 1102 52-53 0.17135178320766187 1102 54-55 0.19155612956259205 1102 56-57 0.1508511581877272 1102 58-59 0.21250629287668232 1102 60-61 0.07738338375711606 1102 62-63 0.05638682824132246 1102 64-65 0.21745797604550177 1102 66-67 0.20555454354938263 1102 68-69 -0.02656409442098351 1102 70-71 -0.3323824165115994 1102 72-73 -0.445078559269227 1102 74-75 -0.1516943337563923 1102 76-77 -0.15487407231177386 1102 78-79 -0.2690673445426057 1102 80-81 -0.2624387880545598 1102 82-83 -0.24325622632260036 1102 84-85 -0.28550581470296876 1102 86-87 -0.12592640130408483 1102 88-89 -0.1601694803090723 1102 90-91 -0.011171436919614308 1102 92-93 -0.1251366430452272 1102 94-95 -0.17645026940646602 1102 96-97 -0.44611612326364636 1102 98-99 -0.6467042456797323 1102 100-101 -0.8504885151834998 1103 1 0.021742473638404647 1103 2 0.047352891424175425 1103 3 0.021197982279822725 1103 4 0.08035156436606883 1103 5 0.06297081643884184 1103 6 0.1106087034293779 1103 7 0.15379733188357392 1103 8 0.15808583579109126 1103 9 0.14231056279606236 1103 10-11 0.12115959487457673 1103 12-13 0.163717468182206 1103 14-15 0.19527323189853973 1103 16-17 0.10716947110356756 1103 18-19 0.20499868673989852 1103 20-21 0.27617872940366794 1103 22-23 0.3021857775208403 1103 24-25 0.16829774585541912 1103 26-27 0.2592578427792418 1103 28-29 0.2801200136104356 1103 30-31 0.29160638602858313 1103 32-33 0.30832285750621935 1103 34-35 0.2499753475203903 1103 36-37 0.3031717526593809 1103 38-39 0.289996353323275 1103 40-41 0.3037323355506416 1103 42-43 0.36245329304498597 1103 44-45 0.2407049438822071 1103 46-47 0.29116004886449076 1103 48-49 0.2376377805624159 1103 50-51 0.21147922981753453 1103 52-53 0.174062496325611 1103 54-55 0.21365875460639216 1103 56-57 0.19966508935460325 1103 58-59 0.2558441557846862 1103 60-61 0.3064509476663062 1103 62-63 0.14044178786189576 1103 64-65 0.025797637891948 1103 66-67 0.290855722176353 1103 68-69 0.03890774310968226 1103 70-71 -0.011687358259976577 1103 72-73 -0.08128881708581659 1103 74-75 -0.12577086891890232 1103 76-77 0.010376589545224135 1103 78-79 0.27157269666304273 1103 80-81 0.10346309538126164 1103 82-83 0.12553494563599443 1103 84-85 -0.07246433129949637 1103 86-87 0.21204637671394266 1103 88-89 0.2147697249468763 1103 90-91 0.5094848591891754 1103 92-93 0.6255426466612128 1103 94-95 0.24541676501713283 1103 96-97 0.24089612709702024 1103 98-99 0.09010710368817598 1103 100-101 -0.1995288664862933 1104 1 0.07759994114156399 1104 2 0.019758909716976802 1104 3 0.16374972099552565 1104 4 0.02055998217736743 1104 5 0.08200442718531065 1104 6 0.08169014495148019 1104 7 0.11880400004242375 1104 8 0.12231377391940867 1104 9 0.142580797962907 1104 10-11 0.09383004747461854 1104 12-13 0.11124034549911954 1104 14-15 0.08129907430361527 1104 16-17 0.13146983791596512 1104 18-19 0.21283508212535907 1104 20-21 0.1561781861036451 1104 22-23 0.09123836698211818 1104 24-25 0.04179316558156643 1104 26-27 0.08995494481127508 1104 28-29 0.057941855912503115 1104 30-31 0.026968058306046316 1104 32-33 0.006415280197032303 1104 34-35 0.2249713788835237 1104 36-37 0.24046941230964336 1104 38-39 0.32473515476380754 1104 40-41 0.314221846040148 1104 42-43 0.21786898971832613 1104 44-45 0.17993245048315742 1104 46-47 0.3298609788979192 1104 48-49 0.028210902661463422 1104 50-51 0.15774006645995087 1104 52-53 0.1266281599340502 1104 54-55 0.11825993984934513 1104 56-57 0.36133377311691817 1104 58-59 0.30429392557545043 1104 60-61 0.32729485106190026 1104 62-63 0.177439387437893 1104 64-65 0.1834420814183133 1104 66-67 0.26170046032979855 1104 68-69 0.2018234702021502 1104 70-71 0.09699542498680103 1104 72-73 -0.029221150200037016 1104 74-75 -0.06766620499948672 1104 76-77 0.12683266614373423 1104 78-79 0.12344321921296597 1104 80-81 -0.006938157151662239 1104 82-83 0.14686753608200576 1104 84-85 0.0459528602978061 1104 86-87 0.12660523265992651 1104 88-89 0.0968412203999911 1104 90-91 0.32507043898792176 1104 92-93 0.10974733041673801 1104 94-95 -0.02695679675876761 1104 96-97 0.08229284392367475 1104 98-99 -0.10719187459651991 1104 100-101 -0.06817236235027835 1105 1 0.061250507778773056 1105 2 0.14567099111445714 1105 3 0.045614239200723716 1105 4 0.1087181449160397 1105 5 0.14127736742276653 1105 6 0.11617025786493684 1105 7 0.054167725877341866 1105 8 0.13273823158321107 1105 9 0.05085931487536044 1105 10-11 0.06670357789131742 1105 12-13 0.049920517695447586 1105 14-15 0.15568335208749318 1105 16-17 0.08469239212474378 1105 18-19 0.07185595974375758 1105 20-21 0.07179047499119662 1105 22-23 0.05041170205235801 1105 24-25 0.1665763056199765 1105 26-27 0.1389709539476769 1105 28-29 0.12665161986841156 1105 30-31 0.11306442996220767 1105 32-33 0.243770612592364 1105 34-35 0.23928192842032558 1105 36-37 0.23013465173463743 1105 38-39 0.31846919213189295 1105 40-41 0.24444220280825135 1105 42-43 0.23384721512903894 1105 44-45 0.2659342952072805 1105 46-47 0.36324209548040187 1105 48-49 0.264499529677785 1105 50-51 0.302677038385589 1105 52-53 0.3229267888657503 1105 54-55 0.35618988559502895 1105 56-57 0.29389273998309307 1105 58-59 0.17964795308513004 1105 60-61 0.20054279092676808 1105 62-63 0.1636470110832633 1105 64-65 0.10652089397237319 1105 66-67 0.165206954123029 1105 68-69 0.13280335431903012 1105 70-71 0.17231779632390953 1105 72-73 -0.03273175625980684 1105 74-75 -0.1333787062345415 1105 76-77 0.19080932171740272 1105 78-79 0.3560749649170276 1105 80-81 0.06232082897868452 1105 82-83 0.18297889405176804 1105 84-85 0.2914956049624813 1105 86-87 0.3800820298169967 1105 88-89 0.43546304808995373 1105 90-91 0.35678567363826375 1105 92-93 0.367344673837362 1105 94-95 0.1532421707035425 1105 96-97 0.1701392747799133 1105 98-99 0.156269450502716 1105 100-101 -0.26728720387898264 1106 1 0.01613090259530736 1106 2 0.00433458234113715 1106 3 0.05458942667033284 1106 4 -0.03736952005295535 1106 5 -0.03880116080217988 1106 6 0.04451325260128414 1106 7 0.057301816767044045 1106 8 0.033312157311975454 1106 9 0.10264006287696503 1106 10-11 0.0878235008560182 1106 12-13 0.08485166153732138 1106 14-15 0.026169098852101058 1106 16-17 -0.0314441237131291 1106 18-19 -0.014788483563215493 1106 20-21 0.053385555566869414 1106 22-23 0.10458727299222659 1106 24-25 0.04865866854748191 1106 26-27 0.02079258695939501 1106 28-29 0.09822379460117503 1106 30-31 0.2035614376998609 1106 32-33 0.2700078266958812 1106 34-35 0.02878091109378289 1106 36-37 0.213511120139934 1106 38-39 0.20855873606248565 1106 40-41 0.12011615498323636 1106 42-43 0.20693924799061136 1106 44-45 0.26840883005231575 1106 46-47 0.30016944940766876 1106 48-49 0.2807546269742929 1106 50-51 0.3464332767087299 1106 52-53 0.3685211997547668 1106 54-55 0.3822975345256694 1106 56-57 0.3787773896559301 1106 58-59 0.3153162854373903 1106 60-61 0.22353554380812568 1106 62-63 0.2680578787753589 1106 64-65 0.3884736384080014 1106 66-67 0.3621665447184128 1106 68-69 -0.11265893208504707 1106 70-71 0.1297004518660998 1106 72-73 0.1421902913604569 1106 74-75 0.13775881200055196 1106 76-77 0.09288417732954457 1106 78-79 0.3176037059914272 1106 80-81 0.4484999573623085 1106 82-83 0.5182082510672714 1106 84-85 0.5229141996442337 1106 86-87 0.4456086829193069 1106 88-89 0.3017888463820384 1106 90-91 0.23661504217067275 1106 92-93 0.41058025211392035 1106 94-95 0.2880603315818995 1106 96-97 0.3479600098996478 1106 98-99 0.26323883046671526 1106 100-101 -0.0178027986204512 1107 1 -0.01843582205524541 1107 2 -0.04133158467713116 1107 3 2.0562884904506973E-4 1107 4 0.038388896863537525 1107 5 0.0675422148470517 1107 6 0.0460453534685783 1107 7 0.04738749721413171 1107 8 0.09591470860303986 1107 9 0.17329327604794287 1107 10-11 0.09262297838418476 1107 12-13 0.05362325128280787 1107 14-15 0.12889734872564418 1107 16-17 0.15908807893439558 1107 18-19 0.09587284118356365 1107 20-21 0.070789589148184 1107 22-23 0.08602973015199922 1107 24-25 0.07978876835378657 1107 26-27 0.1906391147114661 1107 28-29 0.16912259112707773 1107 30-31 0.22024838330604268 1107 32-33 0.12162459020797911 1107 34-35 0.08551452139271021 1107 36-37 0.15235084095368023 1107 38-39 0.13387402345284016 1107 40-41 0.08432806758794698 1107 42-43 0.16936538021854375 1107 44-45 -9.594848372032061E-4 1107 46-47 -0.003426322703319329 1107 48-49 -0.06669801437684697 1107 50-51 -0.09830545652837941 1107 52-53 -0.1439804194761436 1107 54-55 -0.16802430689457282 1107 56-57 -0.24258512376055563 1107 58-59 -0.37706338257400773 1107 60-61 -0.19716609207772962 1107 62-63 -0.31916237980810536 1107 64-65 -0.26835311622145497 1107 66-67 -0.10852344812612813 1107 68-69 -0.10313130029506823 1107 70-71 -0.0737281539298209 1107 72-73 -0.19750970370474263 1107 74-75 -0.21636120539461245 1107 76-77 -0.13210487358524148 1107 78-79 0.12390899116112308 1107 80-81 0.16742498851322551 1107 82-83 0.05237556542630273 1107 84-85 0.05347332778862679 1107 86-87 -0.03842917007303015 1107 88-89 0.18772675118976778 1107 90-91 0.34632911683418044 1107 92-93 0.28911210315701297 1107 94-95 0.28886532066365334 1107 96-97 0.3946937127260526 1107 98-99 0.44647061489002127 1107 100-101 -0.12168571434642317 1108 1 -0.012475221172074669 1108 2 0.005150485365234658 1108 3 0.008165230231966802 1108 4 -0.004133089487744712 1108 5 -0.08017224355349128 1108 6 9.21481413705294E-4 1108 7 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8 574.0 9 1076.0 10 1794.0 11 2626.0 12 3940.0 13 5854.0 14 7351.0 15 9829.0 16 12617.0 17 17255.0 18 17617.0 19 13009.0 20 12315.0 21 13171.0 22 14029.0 23 15308.0 24 17706.0 25 20498.0 26 24121.0 27 28826.0 28 34931.0 29 42027.0 30 51150.0 31 62215.0 32 77424.0 33 100193.0 34 134781.0 35 194555.0 36 297973.0 37 449758.0 38 329066.0 39 23309.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.58499040055701 19.445236396439515 15.218625529151137 22.751147673852337 2 15.660377358490566 20.8316317515167 39.721251447980684 23.786739442012056 3 20.6491371016826 23.840879194235566 29.288575187010384 26.22140851707145 4 13.650334753499695 16.830934757426423 36.369544303300415 33.149186185773466 5 14.349783048318379 36.92610880961263 33.53007873088175 15.194029411187246 6 32.3943219523688 35.761392417489645 16.781114405198988 15.06317122494257 7 29.009826634991065 32.30881746608289 20.15584198849469 18.52551391043135 8 25.77587222429466 35.155596568040366 19.143482614415017 19.925048593249954 9 25.572467751752303 18.25103567431724 20.785836294740147 35.390660279190314 10-11 25.476655606384856 26.46057074980857 26.467957905483676 21.594815738322897 12-13 26.695978049594565 23.596710384230263 27.20620717413072 22.50110439204445 14-15 22.819168152295983 25.234624718254 26.324255287400554 25.621951842049462 16-17 22.331127628671968 28.58403283180181 26.672231240179983 22.41260829934624 18-19 22.029073502971308 27.382555211039605 28.833579817397275 21.75479146859181 20-21 22.94801503936544 25.952746745724774 28.831774095380204 22.267464119529578 22-23 22.46080635344472 26.022691575206647 28.91629689984882 22.600205171499812 24-25 22.88447299296156 26.12268668782363 28.9017658811625 22.091074438052306 26-27 22.54318647954632 26.133799244440613 29.280466264516463 22.042548011496596 28-29 21.839598099464002 27.092332083325154 29.088434806510516 21.97963501070033 30-31 22.32901601391477 26.13746555114849 28.889569541828124 22.643948893108607 32-33 22.241302299098816 26.35813225217443 28.994733277050244 22.405832171676515 34-35 23.102103830535057 26.25943275240628 28.595773194231555 22.042690222827108 36-37 22.748547342993984 25.938231084773307 29.061241122651634 22.25198044958108 38-39 22.272396555939473 26.55255687815492 29.103494627615127 22.07155193829048 40-41 22.547737963278415 26.520305263767654 28.711259080497463 22.220697692456472 42-43 22.33468262913379 26.649467481414867 28.14236527004897 22.87348461940237 44-45 22.682326966182657 26.304924743042534 28.22655599806308 22.786192292711732 46-47 23.04927516597997 26.884411380978886 27.84117069014094 22.225142762900198 48-49 22.9666662903529 26.776131432327087 27.85939968792136 22.39780258939865 50-51 22.197721074185957 27.01007377586088 28.304691532182357 22.4875136177708 52-53 22.979581749450688 27.269409633589625 27.328335099133938 22.42267351782575 54-55 22.367620206938525 26.684860503013763 27.783411540651443 23.164107749396265 56-57 22.819071132664483 26.97268961184301 27.638466220328667 22.56977303516384 58-59 23.446746233798173 26.53630674766221 27.48892308017032 22.5280239383693 60-61 22.80553324040741 26.634910840449432 28.432558631568845 22.126997287574312 62-63 22.495258884422313 26.879345818086946 28.08934576163779 22.536049535852946 64-65 22.250438023938354 27.19304639605512 27.753501062326162 22.80301451768036 66-67 22.507820064278537 26.89765870527469 27.402167897301606 23.192353333145167 68-69 22.205704850020787 26.864143754641677 27.788159424792948 23.14199197054459 70-71 22.73283038501561 26.775175230204383 27.608647635128502 22.883346749651505 72-73 22.676334596430607 26.436004162330907 28.076983488111836 22.810677753126658 74-75 22.209373097991477 27.516246834076142 27.354293876268827 22.920086191663557 76-77 22.39295707068285 26.876462225068487 27.412423892975884 23.31815681127278 78-79 22.549611988357196 26.94027909351056 27.584560032591998 22.92554888554025 80-81 22.6294163440932 26.99174185745378 27.52490843102164 22.853933367431388 82-83 22.531156279603987 26.81474591495649 27.82018936735205 22.833908438087477 84-85 22.327395794475095 27.007686568629374 27.62850214988318 23.03641548701235 86-87 22.40682810557217 26.929973886785742 27.603969483727823 23.059228523914264 88-89 22.1523941538902 27.000942912987636 27.238408169711526 23.608254763410642 90-91 22.43521871152329 26.63673356368651 27.488659302131367 23.43938842265883 92-93 22.72765201342941 26.72930614729154 27.523805784069268 23.019236055209785 94-95 22.3682092160289 26.576139241748965 27.626097029430817 23.429554512791313 96-97 21.89714919600259 26.6103508530815 28.08081204719926 23.411687903716647 98-99 22.0954169977848 26.806604266748607 27.259681861532652 23.83829687393394 100-101 22.156498221304343 25.789749460992017 28.052589017612707 24.001163300090926 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 798.0 1 568.5 2 460.0 3 611.0 4 515.5 5 459.5 6 920.0 7 2006.5 8 3108.5 9 3370.5 10 2728.0 11 2087.0 12 2031.5 13 2202.5 14 2365.5 15 2513.0 16 2640.0 17 2781.5 18 2897.0 19 3104.5 20 3423.5 21 3998.0 22 4891.0 23 6048.5 24 7421.0 25 9181.0 26 11470.0 27 13905.0 28 16366.5 29 19145.0 30 21894.0 31 25023.0 32 28676.5 33 31405.0 34 34076.0 35 36780.5 36 40176.5 37 44508.5 38 47561.0 39 49507.5 40 52093.5 41 56937.0 42 61749.5 43 65126.0 44 68391.5 45 71651.5 46 73930.5 47 83139.0 48 102041.0 49 101679.0 50 85921.0 51 78402.5 52 73291.0 53 71136.5 54 69868.5 55 64387.0 56 57785.5 57 52131.0 58 46734.5 59 40683.0 60 34157.0 61 27700.0 62 22590.5 63 18244.0 64 14639.5 65 11746.0 66 8853.5 67 7271.5 68 5611.0 69 3924.5 70 2900.5 71 2155.0 72 1724.5 73 1425.5 74 1206.5 75 1008.5 76 720.5 77 435.0 78 253.0 79 157.0 80 107.5 81 77.0 82 45.0 83 23.0 84 13.5 85 6.5 86 4.0 87 4.5 88 3.0 89 0.5 90 1.0 91 1.5 92 1.0 93 0.0 94 0.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3177213201657079 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011780181807472562 16-17 0.001325270453340663 18-19 4.908409086446901E-5 20-21 0.0 22-23 0.0 24-25 4.172147723479866E-4 26-27 0.0010553079535860837 28-29 0.0 30-31 0.002969587497300375 32-33 0.0 34-35 0.009939528400054975 36-37 0.02625998861249092 38-39 0.02071348634480592 40-41 0.012860031806490882 42-43 0.010209490899809553 44-45 0.0032640920424871892 46-47 0.0012761863624761941 48-49 6.380931812380971E-4 50-51 2.4542045432234506E-5 52-53 2.4542045432234506E-5 54-55 0.0 56-57 0.0 58-59 0.004343942041505507 60-61 0.010479453399564133 62-63 0.004343942041505507 64-65 0.00223332613433334 66-67 0.004540278404963383 68-69 0.0034849704513772997 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.20838650776510317 78-79 4.908409086446901E-4 80-81 0.0028468772701392025 82-83 4.908409086446901E-4 84-85 0.0 86-87 0.10521174876798932 88-89 0.3912247462352502 90-91 0.3771130701117154 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0015216068167985392 100-101 6.135511358058626E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2037320.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.70626243324903 #Duplication Level Percentage of deduplicated Percentage of total 1 78.19978512611625 43.56217752459116 2 12.920140912966401 14.394655207445284 3 3.9140048585231444 6.541037454417061 4 1.6318666888998432 3.636207761117273 5 0.87237062464363 2.4298253477727716 6 0.5082590291129977 1.6987926515902207 7 0.34466523245324865 1.3440008323460217 8 0.24811158613802475 1.1057095304107585 9 0.181382516323067 0.9093727849586264 >10 1.0326867437301843 10.68703419965409 >50 0.0866687209222015 3.34265235704632 >100 0.05495219850509864 5.580184442124807 >500 0.0030810630742879636 1.17765791833575 >1k 0.001672577097470606 2.034163152633153 >5k 2.640911206532536E-4 0.971451316353578 >10k+ 8.803037355108454E-5 0.585077519203157 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11931 0.5856222881039796 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8171 0.40106610645357627 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6299 0.3091806883552903 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5340 0.2621090452162645 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4597 0.225639565703964 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3624 0.17788074529283568 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3614 0.177389904384191 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3568 0.1751320362044254 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2718 0.13341055896962675 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2678 0.131447195335048 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2597 0.12747138397502603 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2408 0.11819449080164136 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2092 0.10268391808846916 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.908409086446901E-5 0.0 10-11 0.0 0.0 0.0 4.908409086446901E-5 0.0 12-13 0.0 0.0 0.0 4.908409086446901E-5 0.0 14-15 0.0 0.0 0.0 9.816818172893802E-5 0.0 16-17 0.0 0.0 0.0 9.816818172893802E-5 0.0 18-19 0.0 0.0 0.0 9.816818172893802E-5 0.0 20-21 0.0 0.0 0.0 9.816818172893802E-5 0.0 22-23 0.0 0.0 0.0 1.2271022716117252E-4 0.0 24-25 0.0 0.0 0.0 1.4725227259340703E-4 0.0 26-27 0.0 0.0 0.0 3.190465906190486E-4 0.0 28-29 0.0 0.0 0.0 0.001079849999018318 0.0 30-31 0.0 0.0 0.0 0.0039021852237252864 0.0 32-33 0.0 0.0 0.0 0.009865902263758271 0.0 34-35 0.0 0.0 0.0 0.018283823847014706 0.0 36-37 0.0 0.0 0.0 0.03340172383327116 0.0 38-39 0.0 0.0 0.0 0.05831189994698918 0.0 40-41 0.0 0.0 0.0 0.08572536469479512 0.0 42-43 0.0 0.0 0.0 0.1144395578505095 0.0 44-45 0.0 0.0 0.0 0.15228339190701512 0.0 46-47 0.0 0.0 0.0 0.20171107140753539 0.0 48-49 0.0 0.0 0.0 0.2591885418098286 0.0 50-51 0.0 0.0 0.0 0.3220407201617812 0.0 52-53 0.0 0.0 0.0 0.38506469283175937 0.0 54-55 0.0 0.0 0.0 0.45631025072153614 4.908409086446901E-5 56-57 0.0 0.0 0.0 0.5477293699566097 4.908409086446901E-5 58-59 0.0 0.0 0.0 0.649284353955196 4.908409086446901E-5 60-61 0.0 0.0 0.0 0.7604352777177861 4.908409086446901E-5 62-63 0.0 0.0 0.0 0.8753165923860758 9.816818172893802E-5 64-65 0.0 0.0 0.0 1.0004564820450397 9.816818172893802E-5 66-67 0.0 0.0 0.0 1.1438065694147213 9.816818172893802E-5 68-69 0.0 0.0 0.0 1.315944476076414 9.816818172893802E-5 70-71 0.0 0.0 0.0 1.5093112520369898 9.816818172893802E-5 72-73 0.0 0.0 0.0 1.7029234484518878 9.816818172893802E-5 74-75 0.0 0.0 0.0 1.9116780868984744 9.816818172893802E-5 76-77 0.0 0.0 0.0 2.138274792374296 9.816818172893802E-5 78-79 0.0 0.0 0.0 2.4115013841713626 9.816818172893802E-5 80-81 0.0 0.0 0.0 2.7157982054856378 9.816818172893802E-5 82-83 0.0 0.0 0.0 3.018990634755463 9.816818172893802E-5 84-85 0.0 0.0 0.0 3.3342577503779474 9.816818172893802E-5 86-87 0.0 0.0 0.0 3.659587104627648 9.816818172893802E-5 88-89 0.0 0.0 0.0 4.033313372469715 9.816818172893802E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7090 0.0 22.76274 1 TACATGG 7090 0.0 22.419073 2 ACATGGG 6945 0.0 21.72571 3 AGTACTT 7910 0.0 20.246426 12-13 GAGTACT 7755 0.0 19.76386 12-13 AGAGTAC 11600 0.0 19.532887 10-11 TAGACCG 150 7.780817E-4 18.979252 5 CATGGGG 4080 0.0 18.839699 4 GTACTTT 8250 0.0 18.57803 14-15 GTAAGCG 225 0.0 17.92573 94-95 CGCGGAA 385 0.0 17.345602 82-83 ATTCGCG 410 0.0 16.802086 80-81 TACTTTT 9625 0.0 16.56493 14-15 CCGTAAG 230 4.191906E-5 16.558968 1 TCGCGCT 475 0.0 16.48522 60-61 CGATCGT 475 0.0 16.482792 24-25 ATGGGGA 2730 0.0 16.337452 5 TAAGGTG 870 0.0 15.816042 5 CGCGATC 485 0.0 15.653374 22-23 ACTTTTT 9800 0.0 15.6389 16-17 >>END_MODULE