FastQCFastQC Report
Fri 27 May 2016
SRR939070_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939070_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2018892
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT102080.5056238768591881No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80290.39769338825454753No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT60550.2999169841675533No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54200.2684640882226489No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT44910.22244874911585164No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC43190.21392922454494842No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38570.19104538529054552No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT31570.1563729015717532No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT26360.1305666672610521No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC23010.11397340719563008No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG23010.11397340719563008No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC22170.109812709149375No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC20420.10114458821967694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG72650.025.8872131
TACATGG72500.025.288342
ACATGGG72450.024.326153
CATGGGG42750.022.4972154
AGTACTT63900.020.5638712-13
GAGTACT63350.020.10705212-13
AGAGTAC104350.019.3300510-11
GTACTTT66750.018.80027614-15
ATGGGGA26850.018.5273745
GTACCTG16500.018.4213641
CTACACG3105.7716534E-918.3395394
TACCTGG16100.017.6552032
TACTTTT76900.017.05780214-15
TATGACG2555.6477784E-616.7213444
GTATCGT2002.7649163E-416.6224041
ACTTTTT77050.016.44092416-17
GTAAGCG1903.8544385E-916.27011594-95
GTCCTAG7950.016.1295571
GTGTAGC11850.015.630471
CATGGGA33850.015.3958234
Produced by FastQC (version 0.11.5)