##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939070_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2018892 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.33223173899347 34.0 31.0 34.0 31.0 34.0 2 32.452667601833085 34.0 31.0 34.0 31.0 34.0 3 32.5221690907686 34.0 31.0 34.0 31.0 34.0 4 35.946639542877975 37.0 35.0 37.0 35.0 37.0 5 35.97653019577075 37.0 37.0 37.0 35.0 37.0 6 36.059612401257716 37.0 37.0 37.0 35.0 37.0 7 35.99978701188573 37.0 37.0 37.0 35.0 37.0 8 35.99714595926875 37.0 37.0 37.0 35.0 37.0 9 37.71921033913652 39.0 38.0 39.0 35.0 39.0 10-11 37.631413666506184 39.0 38.0 39.0 35.0 39.0 12-13 37.540369668114984 39.0 38.0 39.0 35.0 39.0 14-15 38.875839074106 40.0 38.5 41.0 36.0 41.0 16-17 38.797993899624146 40.0 38.0 41.0 35.5 41.0 18-19 38.69088415824125 40.0 38.0 41.0 35.0 41.0 20-21 38.67786414528365 40.0 38.5 41.0 34.5 41.0 22-23 38.727998327795646 40.0 39.0 41.0 35.0 41.0 24-25 38.67292009676595 40.5 38.5 41.0 35.0 41.0 26-27 38.55871289796582 40.0 38.0 41.0 34.5 41.0 28-29 38.4263043788375 40.0 38.0 41.0 34.0 41.0 30-31 38.231973280393404 40.0 38.0 41.0 34.0 41.0 32-33 38.042731854898626 40.0 38.0 41.0 34.0 41.0 34-35 37.73042490633476 40.0 38.0 41.0 33.0 41.0 36-37 37.47115893272151 40.0 37.5 41.0 33.0 41.0 38-39 37.29283612001038 40.0 37.0 41.0 32.0 41.0 40-41 37.121617946873826 40.0 37.0 41.0 31.5 41.0 42-43 36.91299361233786 40.0 37.0 41.0 31.0 41.0 44-45 36.65490947509822 40.0 36.0 41.0 30.5 41.0 46-47 36.51923332204001 40.0 36.0 41.0 30.0 41.0 48-49 36.39937252710892 40.0 36.0 41.0 30.0 41.0 50-51 35.285594276464515 38.5 34.5 40.0 28.0 40.5 52-53 35.44716532632751 38.5 35.0 40.0 28.5 40.5 54-55 35.97331630419062 39.0 35.5 41.0 29.0 41.0 56-57 35.863860473962944 39.0 35.0 41.0 28.5 41.0 58-59 35.658828704061435 39.0 35.0 41.0 28.0 41.0 60-61 35.34736206790656 39.0 35.0 41.0 28.0 41.0 62-63 34.98561438650508 38.0 35.0 40.0 27.0 41.0 64-65 34.61395730925676 37.5 34.5 40.0 26.0 41.0 66-67 34.21537159986765 37.0 34.0 40.0 26.0 41.0 68-69 33.800656003391964 36.5 34.0 39.0 25.5 41.0 70-71 33.34410384507938 36.0 34.0 39.0 24.5 41.0 72-73 32.89630425996042 35.0 33.0 38.5 23.5 40.0 74-75 32.457149515674935 35.0 33.0 37.0 22.0 39.5 76-77 32.01974375053247 35.0 32.5 37.0 20.5 39.0 78-79 31.585213572593283 35.0 32.0 36.5 19.5 39.0 80-81 31.121183302524358 35.0 31.5 36.0 17.5 37.5 82-83 30.72909249231757 35.0 31.0 35.5 13.0 37.0 84-85 30.355492517678012 34.5 31.0 35.0 7.5 37.0 86-87 29.975133885319273 34.0 31.0 35.0 4.5 36.0 88-89 29.55920425659223 34.0 30.0 35.0 2.0 36.0 90-91 29.14912090394137 34.0 30.0 35.0 2.0 36.0 92-93 28.73805978724964 34.0 29.0 35.0 2.0 35.0 94-95 28.251516178180903 34.0 29.0 35.0 2.0 35.0 96-97 27.662462132694564 34.0 28.0 35.0 2.0 35.0 98-99 26.965271297325465 34.0 26.5 35.0 2.0 35.0 100-101 23.997776255490635 30.0 13.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2190527493336205 1101 2 0.16669463585569844 1101 3 0.15814841318768913 1101 4 0.220583195636344 1101 5 0.0971104237895517 1101 6 0.08834051511939123 1101 7 0.10652221297639386 1101 8 0.1653885860206259 1101 9 0.17100004737734054 1101 10-11 0.2602406724333335 1101 12-13 0.18545368089667136 1101 14-15 0.3122052805207929 1101 16-17 0.3552576246645103 1101 18-19 0.3922828328300909 1101 20-21 0.39851685935653336 1101 22-23 0.4201977295902566 1101 24-25 0.47301054454189284 1101 26-27 0.36187775626492424 1101 28-29 0.31603865708780177 1101 30-31 0.40404421646692157 1101 32-33 0.4450617336836231 1101 34-35 0.42265162231634434 1101 36-37 0.4794189163213929 1101 38-39 0.3297517455513841 1101 40-41 0.40331727620853286 1101 42-43 0.3003556567196455 1101 44-45 0.31868639021787715 1101 46-47 0.4953335902849929 1101 48-49 0.5541187761803954 1101 50-51 0.5936999426121545 1101 52-53 0.6865989155084478 1101 54-55 0.5975680989858674 1101 56-57 0.5891913338257382 1101 58-59 0.5294356567963519 1101 60-61 0.5099763186164239 1101 62-63 0.42898591149619847 1101 64-65 0.4370224422669722 1101 66-67 0.4154088511652887 1101 68-69 0.3521149130599639 1101 70-71 0.35327412389960955 1101 72-73 0.3673315803906547 1101 74-75 0.1925039337732528 1101 76-77 0.179439160557898 1101 78-79 -0.07115687198814058 1101 80-81 0.059257460561955355 1101 82-83 -0.024619312287921247 1101 84-85 -0.0401818243164378 1101 86-87 -0.24522421887801116 1101 88-89 -0.06749476251992093 1101 90-91 -0.03946403888194183 1101 92-93 -0.12358247425818547 1101 94-95 -0.28559105743583046 1101 96-97 -0.2728083369214396 1101 98-99 -0.1689111305129707 1101 100-101 -0.3828703761890324 1102 1 0.17891943698845125 1102 2 0.19105777613536645 1102 3 0.21075281995337747 1102 4 0.04548565687382222 1102 5 0.14439001991723188 1102 6 0.049935915712303824 1102 7 0.12104808651518795 1102 8 0.14146301156322494 1102 9 0.17739675373412922 1102 10-11 0.14407163148016622 1102 12-13 0.21764803133161337 1102 14-15 0.2051787417320341 1102 16-17 0.20037572058792819 1102 18-19 0.25473678066699534 1102 20-21 0.2928742278900245 1102 22-23 0.243933192473051 1102 24-25 0.31649924653613937 1102 26-27 0.25135443878515673 1102 28-29 0.24517641817498514 1102 30-31 0.28910635643551075 1102 32-33 0.39129454493627946 1102 34-35 0.27310389859560047 1102 36-37 0.22361531310271232 1102 38-39 0.19092672690565138 1102 40-41 0.18942723172111897 1102 42-43 0.201222636709673 1102 44-45 0.199194847325181 1102 46-47 0.2833348233398567 1102 48-49 0.28616358591890645 1102 50-51 0.21862545335547168 1102 52-53 0.30138592308292544 1102 54-55 0.29752443610082224 1102 56-57 0.1906962979022424 1102 58-59 0.07990329941282681 1102 60-61 -0.03801678152337473 1102 62-63 0.012081722482747637 1102 64-65 -0.03768681538902996 1102 66-67 0.009874814728327408 1102 68-69 -0.200759341578987 1102 70-71 -0.2492563557924612 1102 72-73 -0.33174306627729067 1102 74-75 -0.42245641358092456 1102 76-77 -0.47886596992479014 1102 78-79 -0.5130599366354431 1102 80-81 -0.4443484753416804 1102 82-83 -0.6724036551146533 1102 84-85 -0.6898649404597137 1102 86-87 -0.658988146963555 1102 88-89 -0.7503888434817796 1102 90-91 -0.855014584761701 1102 92-93 -1.0770969617158812 1102 94-95 -1.1357884773903315 1102 96-97 -1.1565708970281783 1102 98-99 -1.1227207640820467 1102 100-101 -1.4702130117782168 1103 1 0.17380715952609194 1103 2 0.10617543302939936 1103 3 0.15720384895142558 1103 4 0.14313203435969513 1103 5 0.1419559482447852 1103 6 0.15789287056627188 1103 7 0.21687284789377514 1103 8 0.2673592806123537 1103 9 0.22170739895091884 1103 10-11 0.24173345665833068 1103 12-13 0.23992360362318266 1103 14-15 0.2163549956605948 1103 16-17 0.30500112313661987 1103 18-19 0.305347358277416 1103 20-21 0.30706247892047855 1103 22-23 0.31440405025009 1103 24-25 0.3333847857999359 1103 26-27 0.39377489225670814 1103 28-29 0.2553040464029479 1103 30-31 0.2887898301913978 1103 32-33 0.31430264073862446 1103 34-35 0.1911120913246407 1103 36-37 0.11229015553625032 1103 38-39 0.27172270105530316 1103 40-41 0.33649471451057167 1103 42-43 0.15732483864190527 1103 44-45 0.02299756030342337 1103 46-47 0.22176001895005015 1103 48-49 0.1437709988726752 1103 50-51 -0.07314874136540794 1103 52-53 0.05054175213332002 1103 54-55 0.07098130250917478 1103 56-57 0.06932287084078581 1103 58-59 0.13096761512246502 1103 60-61 0.08485749485826233 1103 62-63 -0.10240415408594572 1103 64-65 -0.05431216705120079 1103 66-67 -0.047092942605992505 1103 68-69 -0.033108263148328376 1103 70-71 -0.05434494679330726 1103 72-73 -0.07168268094520869 1103 74-75 -0.007208814334042302 1103 76-77 0.02479604183726991 1103 78-79 -0.144001846042908 1103 80-81 -0.223543705094297 1103 82-83 -0.41934046860103535 1103 84-85 -0.39238842700992294 1103 86-87 -0.39580005828532094 1103 88-89 -0.4657411841403487 1103 90-91 -0.24784637090263928 1103 92-93 -0.30225979856643903 1103 94-95 -0.4811719194027262 1103 96-97 -0.3967658529628757 1103 98-99 -0.4838784202006643 1103 100-101 -0.6757666691859612 1104 1 0.12607645194379558 1104 2 0.10914402302937987 1104 3 0.12332358980748381 1104 4 0.13573522332069388 1104 5 0.11297579411583314 1104 6 0.1633314225362099 1104 7 0.1384138165158646 1104 8 0.10104098001274764 1104 9 0.14521970151294283 1104 10-11 0.207845025720097 1104 12-13 0.17855833154997924 1104 14-15 0.19409101639496384 1104 16-17 0.09598816891114836 1104 18-19 0.23510692328834182 1104 20-21 0.1775404308251609 1104 22-23 0.20259191474925586 1104 24-25 0.1425301528085896 1104 26-27 0.30507140239537733 1104 28-29 0.14573618277147915 1104 30-31 0.17756413236474344 1104 32-33 0.14184821409251924 1104 34-35 0.22769959162922504 1104 36-37 0.1688397149428198 1104 38-39 0.029576389684386584 1104 40-41 0.12938355968448434 1104 42-43 0.2842295353138198 1104 44-45 0.3022754649008448 1104 46-47 0.4114276616756385 1104 48-49 0.38792994888923005 1104 50-51 0.28216414326608685 1104 52-53 0.313495680448554 1104 54-55 0.2236665243986593 1104 56-57 0.23103287377075077 1104 58-59 0.20206742571512848 1104 60-61 0.14055347320627476 1104 62-63 0.1628253960731172 1104 64-65 0.06760832941353812 1104 66-67 0.13912040838237516 1104 68-69 0.013973158232772676 1104 70-71 0.06866462698373255 1104 72-73 -0.13417670067756404 1104 74-75 -0.1651288876484145 1104 76-77 -0.12391055627745473 1104 78-79 -0.13800122320360586 1104 80-81 -0.15505664000462005 1104 82-83 -0.29433979999968685 1104 84-85 -0.3433529067459524 1104 86-87 -0.092474643173027 1104 88-89 -0.26408774785627287 1104 90-91 -0.22715226760996998 1104 92-93 -0.09664943090701428 1104 94-95 -0.08652492011530555 1104 96-97 -0.03582007623531425 1104 98-99 -0.007047982852174783 1104 100-101 -0.38538121604956643 1105 1 -0.0034156433170480227 1105 2 0.08856032257933322 1105 3 0.05232083027308221 1105 4 0.04836399030701699 1105 5 0.0966977971741727 1105 6 0.14534503262785847 1105 7 -0.009568628730704631 1105 8 0.030854238167954406 1105 9 0.046432288816319556 1105 10-11 -0.0166537731606482 1105 12-13 0.06516097502083795 1105 14-15 0.05956032885683271 1105 16-17 0.09074513999870959 1105 18-19 0.08731983820899814 1105 20-21 0.010277982881582659 1105 22-23 0.01099862580321087 1105 24-25 0.005125394491876989 1105 26-27 -0.0347107854252684 1105 28-29 0.010094996583731586 1105 30-31 0.15586316193184047 1105 32-33 0.026727769362878462 1105 34-35 -0.0023653872370132945 1105 36-37 0.04259310529174343 1105 38-39 0.00575311053227523 1105 40-41 0.03150364971151731 1105 42-43 0.017386564260263526 1105 44-45 0.09833760326482377 1105 46-47 -0.03610012456231004 1105 48-49 0.08161687644143711 1105 50-51 -0.0068159008945301025 1105 52-53 0.09896169080494843 1105 54-55 0.10734207899245263 1105 56-57 0.07007151157444014 1105 58-59 0.13773327684434378 1105 60-61 -0.05306444437536584 1105 62-63 -0.12920347689177447 1105 64-65 0.10432898137478475 1105 66-67 0.054457996802305786 1105 68-69 0.014080346997083382 1105 70-71 -0.10459880698121538 1105 72-73 -0.04879407856953577 1105 74-75 -0.10242259610016191 1105 76-77 0.025633041455790817 1105 78-79 0.09273555836154301 1105 80-81 -0.03891891051044993 1105 82-83 -0.07003881049179128 1105 84-85 -0.0030023591467944755 1105 86-87 -0.01197256754328535 1105 88-89 0.11890750385435211 1105 90-91 0.15897969616599283 1105 92-93 -0.18284619778268763 1105 94-95 -0.15980861045157724 1105 96-97 -0.16503598754295368 1105 98-99 -0.05228221767567476 1105 100-101 0.1298531311492468 1106 1 0.20226767815126578 1106 2 0.22627408300729712 1106 3 0.2754290046957877 1106 4 0.2569153750585116 1106 5 0.24246060275602588 1106 6 0.2693034797156315 1106 7 0.25096896034334293 1106 8 0.21197102452157424 1106 9 0.288198909635625 1106 10-11 0.2759782568636169 1106 12-13 0.28262093921843245 1106 14-15 0.3309727269962224 1106 16-17 0.3672799091146359 1106 18-19 0.3315684368695173 1106 20-21 0.3966748643396656 1106 22-23 0.34774945347474784 1106 24-25 0.3442133315681062 1106 26-27 0.4086727496134017 1106 28-29 0.4232874241667872 1106 30-31 0.3722144955370297 1106 32-33 0.282460509214836 1106 34-35 0.2123784320092028 1106 36-37 0.31978199410382757 1106 38-39 0.317277892296552 1106 40-41 0.46992779076472146 1106 42-43 0.4870698027886249 1106 44-45 0.407737463649255 1106 46-47 0.3736262427372381 1106 48-49 0.19366279752407678 1106 50-51 0.17425029209289988 1106 52-53 0.15705849554358764 1106 54-55 0.21967718401516123 1106 56-57 0.2886656795140041 1106 58-59 0.3599255178125347 1106 60-61 0.43785819339133525 1106 62-63 0.3727550116659728 1106 64-65 0.2188883974775777 1106 66-67 0.32359618166161397 1106 68-69 0.15150091472099803 1106 70-71 0.17178418203615564 1106 72-73 0.18190849187757863 1106 74-75 0.15474707829144307 1106 76-77 0.34588504065403214 1106 78-79 0.27257092257157467 1106 80-81 0.18355339100389756 1106 82-83 0.31570892766680103 1106 84-85 0.35555061528696896 1106 86-87 0.4473881037173335 1106 88-89 0.4544926106049729 1106 90-91 0.45921579854153194 1106 92-93 0.32901338489115517 1106 94-95 0.545478258081701 1106 96-97 0.6192587810200472 1106 98-99 0.44279584954714934 1106 100-101 0.44804047866070107 1107 1 0.09322083810184978 1107 2 0.08127892392473512 1107 3 0.0870351877158555 1107 4 0.027915274901594955 1107 5 0.13149342083769255 1107 6 0.15773936259881793 1107 7 0.17620649915414788 1107 8 0.18233392640118495 1107 9 0.1592796027214618 1107 10-11 0.07173227148651762 1107 12-13 0.1596763259910432 1107 14-15 0.19714773450799328 1107 16-17 0.1476999004343753 1107 18-19 0.10657528092519897 1107 20-21 0.07180389982860191 1107 22-23 0.18153838947448264 1107 24-25 0.2755637806049265 1107 26-27 0.20781607415518266 1107 28-29 0.21626575688805616 1107 30-31 0.21961008195575005 1107 32-33 0.1971742273732744 1107 34-35 0.24936370894306492 1107 36-37 0.20636697724408748 1107 38-39 0.2318760961492501 1107 40-41 0.2770249095842061 1107 42-43 0.2903188930300047 1107 44-45 0.2369231879506586 1107 46-47 0.28844145323368764 1107 48-49 0.26691288933458424 1107 50-51 0.2383440389629854 1107 52-53 0.333053554469231 1107 54-55 0.29835778362120635 1107 56-57 0.2255581443756185 1107 58-59 0.30438717440785723 1107 60-61 0.2115015680115775 1107 62-63 0.19671388056057282 1107 64-65 0.1812460911977425 1107 66-67 0.21389698625547737 1107 68-69 0.17585840812733977 1107 70-71 0.35087742812168443 1107 72-73 0.35614345999429986 1107 74-75 0.30699293012597906 1107 76-77 0.36174588931694274 1107 78-79 0.23767892510888444 1107 80-81 0.2288256508776989 1107 82-83 0.205374737435438 1107 84-85 0.2509727766731409 1107 86-87 0.2463237695938716 1107 88-89 0.24352008698463123 1107 90-91 0.3009825664779662 1107 92-93 0.296563212955121 1107 94-95 0.3916465884659708 1107 96-97 0.34404694915011547 1107 98-99 0.2791100751672886 1107 100-101 0.33960726662851926 1108 1 0.022148570739339846 1108 2 -8.737967372667299E-4 1108 3 0.10706852539608036 1108 4 0.1541845147585903 1108 5 0.014965445979100878 1108 6 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-0.7429286998978988 2316 88-89 -0.8707105631845877 2316 90-91 -0.9375242153712584 2316 92-93 -0.9074243135360724 2316 94-95 -0.7868022362930347 2316 96-97 -0.8489078167800592 2316 98-99 -1.0793509042226397 2316 100-101 -0.8273652674256695 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5389.0 3 1553.0 4 1385.0 5 1522.0 6 1737.0 7 2118.0 8 2643.0 9 3193.0 10 3737.0 11 4804.0 12 6563.0 13 11237.0 14 9299.0 15 9160.0 16 11173.0 17 15916.0 18 13556.0 19 9746.0 20 9605.0 21 10025.0 22 10662.0 23 11610.0 24 13076.0 25 14926.0 26 17042.0 27 19999.0 28 23775.0 29 29044.0 30 35497.0 31 44940.0 32 57405.0 33 77121.0 34 109802.0 35 168018.0 36 274659.0 37 449249.0 38 447063.0 39 80600.0 40 43.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.63105743846234 18.56729492833243 14.62560344441848 23.176044188786747 2 15.038744160607648 19.89918138177837 40.77208655168293 24.289987905931053 3 20.439169086494978 23.509673362847305 28.916948318814363 27.134209231843354 4 13.098818765066708 16.025002368024186 36.74523878362408 34.13094008328502 5 14.521622693909938 36.529260067446934 33.55351120809363 15.395606030549494 6 32.658287828313945 34.99246360548449 17.226101252860534 15.123147313341029 7 29.137139628028713 32.044701986754966 20.284877265336874 18.533281119879447 8 25.79434258457448 34.75314795161947 19.43201797540724 20.020491488398815 9 25.719990304462677 18.270982337368018 20.91410675896924 35.094920599200066 10-11 25.45875055484275 26.11588759846213 26.688512580277163 21.736849266417956 12-13 26.695886054302058 23.369743558731066 27.26090051363599 22.67346987333088 14-15 22.9504666152501 24.958389260414332 26.329660155003104 25.761483969332467 16-17 22.51995195965642 28.301125518037185 26.683357530294117 22.495564992012277 18-19 22.14032987447222 27.22794155008037 28.76193592122075 21.869792654226664 20-21 23.03736941820774 25.89794965394648 28.628133987799643 22.43654694004614 22-23 22.517895484126203 25.901219009790648 28.821904796371477 22.758980709711672 24-25 22.981493339798803 25.974202086055687 28.689376625198914 22.3549279489466 26-27 22.558908869501064 26.063344512515595 29.095358887449898 22.282387730533447 28-29 21.93130063927828 26.925792355036933 28.941274054741733 22.20163295094305 30-31 22.336638071104616 26.106180761067137 28.70189169942114 22.855289468407104 32-33 22.19586537982482 26.276251600930305 28.877771093550347 22.650111925694524 34-35 23.107780869269646 26.208833229887894 28.40240655994808 22.280979340894376 36-37 22.74265291039614 25.934048263111947 28.79280486678643 22.53049395970548 38-39 22.35753398542476 26.47602839788295 28.81226347334003 22.354174143352264 40-41 22.61977064367414 26.48351235494174 28.589051297024433 22.307665704359692 42-43 22.31405781411381 26.544716964696807 28.067395876109057 23.073829345080323 44-45 22.58819166332725 26.272292149429894 28.130060457927108 23.009455729315746 46-47 22.956446249294082 26.780836459201602 27.767267648078548 22.495449643425772 48-49 22.966804679733187 26.679840716625836 27.786898997235593 22.566455606405388 50-51 22.22117957461637 26.96212866050205 28.226331968488257 22.590359796393326 52-53 22.87617831071959 27.17332866057881 27.34788491483507 22.60260811386653 54-55 22.28319182726877 26.573796078448943 27.873247600892913 23.26976449338937 56-57 22.757063412085245 26.905261324475298 27.578248049863767 22.759427213575687 58-59 23.32446621913117 26.504759729567944 27.493627072663063 22.67714697863782 60-61 22.842229988897053 26.55101200933493 28.371944980112918 22.234813021655096 62-63 22.450389927628596 26.832241781508138 28.114232445542275 22.603135845320992 64-65 22.180281749040535 27.133591580884236 27.789673450103976 22.896453219971256 66-67 22.47855971042851 26.91381812659791 27.319297878749403 23.288324284224178 68-69 22.18768284636548 26.75078118191891 27.857880911773126 23.203655059942484 70-71 22.68394851794266 26.71450214052127 27.608031280615553 22.99351806092052 72-73 22.653442858501336 26.376852145504042 28.129709556500735 22.839995439493883 74-75 22.228703055758654 27.369066209805244 27.464088008557685 22.938142725878414 76-77 22.426941738813372 26.794545845980007 27.41283595351251 23.36567646169411 78-79 22.541813493084454 26.911347780671314 27.545224859029222 23.001613867215006 80-81 22.690608401588097 26.942987001837682 27.465849807584398 22.900554788989822 82-83 22.57409011269064 26.74528630589734 27.815424282244255 22.865199299167763 84-85 22.41385317324629 26.937591238514464 27.53445848184089 23.114097106398354 86-87 22.44281234026054 26.87199092542995 27.656832730170404 23.028364004139103 88-89 22.3141557239141 26.92342728005814 27.1866257827511 23.575791213276652 90-91 22.480815441145904 26.64051720082058 27.464705813530593 23.41396154450292 92-93 22.9076335294453 26.646067151399215 27.5055060801514 22.940793239004083 94-95 22.529086773154287 26.487471864157335 27.653581497979395 23.329859864708975 96-97 22.206774107543605 26.424043030785672 28.05858356582262 23.310599295848103 98-99 22.346459111732795 26.70878207603228 27.28295864360401 23.661800168630915 100-101 22.43387238918543 25.727989216394125 28.006264884763404 23.831873509657036 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2039.0 1 1206.5 2 489.0 3 629.5 4 538.5 5 500.0 6 920.5 7 1945.5 8 3172.0 9 3484.0 10 2845.0 11 2426.0 12 2459.0 13 2574.0 14 2781.5 15 3047.5 16 3246.0 17 3295.0 18 3332.5 19 3435.0 20 3575.0 21 3969.0 22 4681.5 23 5852.5 24 7186.5 25 8661.0 26 10845.5 27 13448.0 28 15755.5 29 18428.5 30 21256.0 31 24284.0 32 27719.5 33 30535.5 34 33647.5 35 36644.0 36 39714.5 37 43549.0 38 46546.0 39 48138.0 40 50565.5 41 55711.0 42 60565.0 43 63808.5 44 67573.5 45 71176.0 46 73164.5 47 81473.5 48 99969.5 49 100170.5 50 84790.0 51 77166.0 52 72460.5 53 71177.5 54 70066.0 55 64622.0 56 57817.5 57 52064.0 58 46542.0 59 40521.0 60 34256.0 61 27959.0 62 22839.5 63 18468.5 64 14693.5 65 11709.5 66 8974.0 67 7373.5 68 5823.5 69 4084.5 70 3079.5 71 2359.0 72 1882.5 73 1569.0 74 1243.0 75 1033.5 76 771.5 77 480.0 78 299.0 79 229.0 80 157.5 81 106.5 82 83.5 83 56.0 84 41.0 85 34.5 86 23.5 87 12.0 88 9.0 89 11.0 90 10.0 91 6.0 92 7.0 93 7.0 94 4.5 95 3.0 96 1.0 97 1.0 98 1.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3725806036182223 2 0.1090201952358026 3 0.10996130550816982 4 0.12100696817858508 5 0.12343404203890056 6 0.06681882933807257 7 0.07588320722455684 8 0.07276268368986553 9 0.07276268368986553 10-11 0.0707566328461354 12-13 0.07479350059339479 14-15 0.06855245352401218 16-17 0.07038514194914834 18-19 0.07972194649342312 20-21 0.07796355624768438 22-23 0.08732512685175829 24-25 0.10022824400710885 26-27 0.10144178093726658 28-29 0.09559699082467017 30-31 0.0860620578020023 32-33 0.0844770299748575 34-35 0.32488117244508374 36-37 0.5080261846597044 38-39 0.488832488315373 40-41 0.4612183316393349 42-43 0.48873342407617637 44-45 0.49814452679984866 46-47 0.49450391600937543 48-49 0.5025033533244968 50-51 0.5011164539757451 52-53 0.499481894029002 54-55 0.49371140209580305 56-57 0.4664439702569528 58-59 0.4566366105764944 60-61 0.4044049904601138 62-63 0.4791241928741112 64-65 0.5064659228923587 66-67 0.5154807686592447 68-69 0.5308357257347099 70-71 0.5260063440738781 72-73 0.5123107130049552 74-75 0.5154064704798473 76-77 0.49356280573700817 78-79 0.4672364841705252 80-81 0.47399761849568967 82-83 0.4879904422822023 84-85 0.48219518428920416 86-87 0.4834830193987593 88-89 0.49294365424202974 90-91 0.5107009191180113 92-93 0.5166447734698042 94-95 0.5118153918089724 96-97 0.5139700390114974 98-99 0.5133261214567199 100-101 0.49118526399629103 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2018892.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.70713068151358 #Duplication Level Percentage of deduplicated Percentage of total 1 79.00595558831411 44.80201048164387 2 12.432238377775446 14.099931327044816 3 3.8071188422455875 6.476723571218197 4 1.564503619484704 3.5487404480728046 5 0.8407655654148598 2.383870139524856 6 0.4876598265771158 1.6592273708299652 7 0.336817435524466 1.3369965232468721 8 0.2261181576212939 1.0258009530955041 9 0.1743296329803342 0.88971599511685 >10 0.982008603512546 10.374079133781814 >50 0.08717102749865187 3.4236257886042667 >100 0.04971370111632804 5.161375870327107 >500 0.0036747518945103286 1.4143859085045776 >1k 0.0015748936690758528 1.9293440361352985 >5k 2.624822781793088E-4 0.9676985568272093 >10k+ 8.749409272643628E-5 0.5064738960260537 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10208 0.5056238768591881 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8029 0.39769338825454753 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6055 0.2999169841675533 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5420 0.2684640882226489 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4491 0.22244874911585164 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4319 0.21392922454494842 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3857 0.19104538529054552 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3157 0.1563729015717532 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2636 0.1305666672610521 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2301 0.11397340719563008 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2301 0.11397340719563008 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2217 0.109812709149375 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2042 0.10114458821967694 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.476605979913735E-5 0.0 22-23 0.0 0.0 0.0 4.95321195982747E-5 0.0 24-25 0.0 0.0 0.0 1.981284783930988E-4 0.0 26-27 0.0 0.0 0.0 3.219587773887855E-4 0.0 28-29 0.0 0.0 0.0 0.0012630690497560048 0.0 30-31 0.0 0.0 0.0 0.004457890763844723 0.0 32-33 0.0 0.0 0.0 0.010277914816642 0.0 34-35 0.0 0.0 0.0 0.018946035746340073 0.0 36-37 0.0 0.0 0.0 0.03385520374542075 0.0 38-39 0.0 0.0 0.0 0.060553016208890816 0.0 40-41 0.0 0.0 0.0 0.08982649889147117 0.0 42-43 0.0 0.0 0.0 0.12014015608561529 0.0 44-45 0.0 0.0 0.0 0.16053359961800828 0.0 46-47 0.0 0.0 0.0 0.21298811427258119 0.0 48-49 0.0 0.0 0.0 0.27116358874075486 0.0 50-51 0.0 0.0 0.0 0.3333511648963887 0.0 52-53 0.0 0.0 0.0 0.3980401130917355 0.0 54-55 0.0 0.0 0.0 0.4702827095258191 0.0 56-57 0.0 0.0 0.0 0.5605549974936748 0.0 58-59 0.0 0.0 0.0 0.6633341456600947 0.0 60-61 0.0 0.0 0.0 0.7765150389421525 0.0 62-63 0.0 0.0 0.0 0.8959122132337936 0.0 64-65 0.0 0.0 0.0 1.0168696492927805 0.0 66-67 0.0 0.0 0.0 1.1659365632237881 0.0 68-69 0.0 0.0 0.0 1.3374662933926134 0.0 70-71 0.0 0.0 0.0 1.5258864763444504 0.0 72-73 0.0 0.0 0.0 1.7229500141661862 0.0 74-75 0.0 0.0 0.0 1.9296723153095856 0.0 76-77 0.0 0.0 0.0 2.1560588679335 0.0 78-79 0.0 0.0 0.0 2.4336368661622316 0.0 80-81 0.0 0.0 0.0 2.741330393106714 0.0 82-83 0.0 0.0 0.0 3.0533579805160453 0.0 84-85 0.0 0.0 0.0 3.370115885347012 0.0 86-87 0.0 0.0 0.0 3.704135733857978 0.0 88-89 0.0 0.0 0.0 4.0801340537284805 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7265 0.0 25.887213 1 TACATGG 7250 0.0 25.28834 2 ACATGGG 7245 0.0 24.32615 3 CATGGGG 4275 0.0 22.497215 4 AGTACTT 6390 0.0 20.56387 12-13 GAGTACT 6335 0.0 20.107052 12-13 AGAGTAC 10435 0.0 19.33005 10-11 GTACTTT 6675 0.0 18.800276 14-15 ATGGGGA 2685 0.0 18.527374 5 GTACCTG 1650 0.0 18.421364 1 CTACACG 310 5.7716534E-9 18.339539 4 TACCTGG 1610 0.0 17.655203 2 TACTTTT 7690 0.0 17.057802 14-15 TATGACG 255 5.6477784E-6 16.721344 4 GTATCGT 200 2.7649163E-4 16.622404 1 ACTTTTT 7705 0.0 16.440924 16-17 GTAAGCG 190 3.8544385E-9 16.270115 94-95 GTCCTAG 795 0.0 16.129557 1 GTGTAGC 1185 0.0 15.63047 1 CATGGGA 3385 0.0 15.395823 4 >>END_MODULE