FastQCFastQC Report
Fri 27 May 2016
SRR939070_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939070_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2018892
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT116810.5785846890274468No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80520.39883262700530786No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61260.3034337646590308No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT50970.2524652135924061No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43990.2178917941128104No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC37940.1879248617558542No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT37130.18391276006839397No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT35060.17365961131155108No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG26800.1327460805233762No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC25980.12868444671631768No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC25570.1266536298127884No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT24280.12026398638461096No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA20590.1019866342528476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGCG4100.028.93402194-95
GTACATG66700.021.6385921
GAGTACT77400.021.12086312-13
TACATGG67600.020.8479022
GTACTTT81150.020.20332714-15
AGTACTT79200.020.07164612-13
ACATGGG68400.019.771583
GTGTAGC9700.019.6426661
AGAGTAC116600.019.12769710-11
ATTCGCG3400.018.86404480-81
CTAGGAC9000.018.4534743
TAGGACC6500.018.2506894
ACTTTTT93700.017.54775816-17
ATGGGGA25800.017.2886375
CATGGGG40650.017.276424
CGATCGT3850.017.25605224-25
TACTTTT93800.016.77042614-15
CGCGATC4150.016.58036222-23
TAAGGTG7900.016.2177015
AATTCGC4200.015.81961578-79
Produced by FastQC (version 0.11.5)