##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939070_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2018892 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.3528316522132 34.0 31.0 34.0 31.0 34.0 2 32.59316991696436 34.0 31.0 34.0 31.0 34.0 3 32.70488664079109 34.0 31.0 34.0 31.0 34.0 4 36.189920015533275 37.0 35.0 37.0 35.0 37.0 5 36.14265151380064 37.0 35.0 37.0 35.0 37.0 6 36.1863284415412 37.0 36.0 37.0 35.0 37.0 7 36.13701178666318 37.0 36.0 37.0 35.0 37.0 8 36.10881909483023 37.0 36.0 37.0 35.0 37.0 9 37.82487770519671 39.0 38.0 39.0 35.0 39.0 10-11 37.72174489769636 39.0 37.5 39.0 35.0 39.0 12-13 37.613306952526436 39.0 37.0 39.0 35.0 39.0 14-15 38.967214690037906 40.0 38.0 41.0 35.5 41.0 16-17 39.098266029089224 40.0 38.0 41.0 36.0 41.0 18-19 39.11992345306237 40.0 38.5 41.0 36.0 41.0 20-21 39.08023633755545 40.0 39.0 41.0 35.5 41.0 22-23 38.996297226399435 40.0 38.5 41.0 35.0 41.0 24-25 38.895639538915404 40.0 38.0 41.0 35.0 41.0 26-27 38.72378760230859 40.0 38.0 41.0 34.5 41.0 28-29 38.54867174668085 40.0 38.0 41.0 34.0 41.0 30-31 38.33864218591188 40.0 38.0 41.0 34.0 41.0 32-33 38.22641503359269 40.0 38.0 41.0 34.0 41.0 34-35 38.081778767759744 40.0 38.0 41.0 33.5 41.0 36-37 37.895129358083544 40.0 37.5 41.0 33.0 41.0 38-39 37.747964229884516 40.0 37.0 41.0 33.0 41.0 40-41 37.53746015140978 40.0 37.0 41.0 33.0 41.0 42-43 37.30507649740551 40.0 37.0 41.0 32.0 41.0 44-45 37.00892197304264 40.0 36.0 41.0 31.0 41.0 46-47 36.66121367562009 39.0 36.0 41.0 30.5 41.0 48-49 36.27383436062949 39.0 35.0 40.5 30.0 41.0 50-51 35.963867309395454 39.0 35.0 40.0 29.0 41.0 52-53 35.65341806297712 38.5 35.0 40.0 28.0 41.0 54-55 35.34316570673418 38.0 35.0 40.0 28.0 41.0 56-57 35.00340855281016 38.0 34.0 40.0 26.5 41.0 58-59 34.58825038684586 38.0 34.0 40.0 26.0 41.0 60-61 34.12285104899123 37.0 33.0 40.0 25.5 41.0 62-63 33.65843219944405 36.5 33.0 39.5 23.5 41.0 64-65 33.12376095402825 36.0 32.0 39.0 22.0 40.0 66-67 32.621880714768295 35.5 31.5 39.0 21.5 40.0 68-69 32.04022528198635 35.0 31.0 38.0 19.5 40.0 70-71 31.445849257909785 35.0 30.5 37.5 17.5 39.5 72-73 30.764321964721244 34.0 29.5 37.0 12.0 39.0 74-75 30.142437782704572 34.0 29.0 36.0 8.0 39.0 76-77 28.212459606556468 31.5 26.5 34.5 7.0 36.0 78-79 29.205795555185716 33.5 29.0 35.0 4.5 37.0 80-81 29.16789778749928 34.0 29.0 35.0 2.0 37.0 82-83 28.691972626569424 34.0 28.5 35.0 2.0 36.5 84-85 28.10538206105131 33.0 27.5 35.0 2.0 36.0 86-87 27.26802127107344 32.5 26.0 35.0 2.0 35.5 88-89 26.450643719426296 32.0 24.5 34.0 2.0 35.0 90-91 25.7449697655942 31.5 23.5 34.0 2.0 35.0 92-93 25.116627338163703 31.0 20.0 34.0 2.0 35.0 94-95 24.33809436066912 31.0 16.0 34.0 2.0 35.0 96-97 23.255825472585954 31.0 2.0 34.0 2.0 35.0 98-99 21.72390623173503 30.0 2.0 34.0 2.0 35.0 100-101 16.10095933809238 14.0 2.0 29.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.04754203245879296 1101 2 0.1175077596931473 1101 3 0.15836967349316922 1101 4 0.14386062214479978 1101 5 0.14687991083206242 1101 6 0.13267487979652515 1101 7 0.15981005655833513 1101 8 0.13454532049555468 1101 9 0.21529152050155886 1101 10-11 0.2340129318519999 1101 12-13 0.23912325393285272 1101 14-15 0.2827915053390626 1101 16-17 0.22175290313387563 1101 18-19 0.24071160880109943 1101 20-21 0.27619353696248794 1101 22-23 0.3221591677008888 1101 24-25 0.19746296478472658 1101 26-27 0.18816032786133263 1101 28-29 0.22469963116170533 1101 30-31 0.33866796192232584 1101 32-33 0.35237793281213214 1101 34-35 0.34612980757250966 1101 36-37 0.3513397714680906 1101 38-39 0.381353553340702 1101 40-41 0.43149496541435184 1101 42-43 0.4091946658029073 1101 44-45 0.5200081990993652 1101 46-47 0.5108146320035587 1101 48-49 0.484663387310853 1101 50-51 0.33712545175639264 1101 52-53 0.34832322269308236 1101 54-55 0.2695400896859397 1101 56-57 0.3544675975388287 1101 58-59 0.3211483799071999 1101 60-61 0.24557768202490848 1101 62-63 0.0762101203167802 1101 64-65 0.030880833137253205 1101 66-67 -0.039600761593071354 1101 68-69 0.20416231266310803 1101 70-71 0.2206053503095795 1101 72-73 0.3528158414240501 1101 74-75 0.3376012737864542 1101 76-77 0.3972860865073109 1101 78-79 0.19597180889646637 1101 80-81 0.3981183248653224 1101 82-83 0.2650787058847506 1101 84-85 0.36500255921174585 1101 86-87 0.4692422255962576 1101 88-89 0.3286755957378169 1101 90-91 0.4586135231197126 1101 92-93 0.36418443724150507 1101 94-95 0.2803307664339094 1101 96-97 -0.06560636238050321 1101 98-99 -0.2333761174489375 1101 100-101 -0.9289580555038732 1102 1 0.04996129360032597 1102 2 0.04205248510419324 1102 3 0.10889287853633078 1102 4 0.053637735297257905 1102 5 0.07377175055741958 1102 6 0.09974500651258467 1102 7 0.17140976722144785 1102 8 0.2181625562788838 1102 9 0.25353683470567745 1102 10-11 0.17091772052738463 1102 12-13 0.20438361965167928 1102 14-15 0.20091094675396448 1102 16-17 0.21700573559957093 1102 18-19 0.08433242381662609 1102 20-21 0.173021231599634 1102 22-23 0.062550522074865 1102 24-25 0.054580858322246684 1102 26-27 0.01207053598150054 1102 28-29 0.10473819692025899 1102 30-31 -0.020930095922565783 1102 32-33 -0.05478971392702192 1102 34-35 0.057350419452568246 1102 36-37 -0.06559814852950296 1102 38-39 0.03115098004138872 1102 40-41 -0.01360036209476334 1102 42-43 -0.03812503271705481 1102 44-45 -0.08847720475421283 1102 46-47 -0.07811804469995565 1102 48-49 0.019590884434343536 1102 50-51 -0.0994106385227127 1102 52-53 0.022238303066529852 1102 54-55 -0.2374623689280213 1102 56-57 -0.15688756348914268 1102 58-59 -0.4162629357136183 1102 60-61 -0.6050601146672037 1102 62-63 -0.611333683114033 1102 64-65 -0.3518084257930312 1102 66-67 -0.3539177841216201 1102 68-69 -0.3981376719705523 1102 70-71 -0.6646498058461354 1102 72-73 -0.419966158973228 1102 74-75 -0.6072551259182113 1102 76-77 -0.6643732155611488 1102 78-79 -0.4396956148433979 1102 80-81 -0.26301973980071836 1102 82-83 -0.5123220969126727 1102 84-85 -0.5650937240085874 1102 86-87 -0.37930378159218847 1102 88-89 -0.44175132608500434 1102 90-91 -0.5262056600657665 1102 92-93 -0.9745706833092846 1102 94-95 -0.8992066131183662 1102 96-97 -0.899719724722484 1102 98-99 -0.9761047479335048 1102 100-101 -1.0239154607666556 1103 1 0.11573453843958248 1103 2 0.13287704051355576 1103 3 0.12847752340902474 1103 4 0.12743651062844918 1103 5 0.1361961780235319 1103 6 0.11515089917111254 1103 7 0.156468516915524 1103 8 0.17349267649221645 1103 9 0.21457836052573498 1103 10-11 0.1220106296125607 1103 12-13 0.17012518157693535 1103 14-15 0.0821851329937715 1103 16-17 0.16579360667737575 1103 18-19 0.2296767316752053 1103 20-21 0.18469272036946904 1103 22-23 0.22461559381273588 1103 24-25 0.11953113492680245 1103 26-27 0.11883347508973685 1103 28-29 0.17511606564758608 1103 30-31 0.18191052266361396 1103 32-33 0.2739741314458186 1103 34-35 0.2635922564073141 1103 36-37 0.32704372186496045 1103 38-39 0.3670080422416788 1103 40-41 0.387853053723191 1103 42-43 0.23060726301599033 1103 44-45 0.3108771422869765 1103 46-47 0.3664307978741306 1103 48-49 0.34776711170806607 1103 50-51 0.3556620201523515 1103 52-53 0.32578848565219687 1103 54-55 0.13311656211590872 1103 56-57 -0.014561007755084177 1103 58-59 0.058550359236740235 1103 60-61 0.0374478757273593 1103 62-63 -0.014601142641076592 1103 64-65 -0.0054505675822511535 1103 66-67 -0.18046958053678708 1103 68-69 -0.2673002616580362 1103 70-71 -0.34607386499081016 1103 72-73 -0.36840569973453796 1103 74-75 -0.28450982262246427 1103 76-77 -0.25206098074653127 1103 78-79 -0.2272580416940322 1103 80-81 -0.1435821206111747 1103 82-83 -0.24773705221734943 1103 84-85 -0.4589807995605284 1103 86-87 -0.5022310647611121 1103 88-89 -0.32796847096376425 1103 90-91 -0.09233268734820754 1103 92-93 -0.23357547633444042 1103 94-95 -0.26739084896040666 1103 96-97 -0.802375940268071 1103 98-99 -0.934319346063301 1103 100-101 -0.9779085983565245 1104 1 0.11354330894410225 1104 2 0.1217428964986027 1104 3 0.0828459269138051 1104 4 0.07752229961072743 1104 5 0.08680941918299112 1104 6 -0.027085866533738567 1104 7 -0.05776205367778431 1104 8 0.054188639982427844 1104 9 0.11198935779962227 1104 10-11 0.17450163023838172 1104 12-13 0.08366566642880002 1104 14-15 -0.003671898047755917 1104 16-17 -0.007184706272035157 1104 18-19 0.027408033569820134 1104 20-21 0.11884098465064596 1104 22-23 0.14429874214655314 1104 24-25 0.13264686476906462 1104 26-27 0.20526342320032853 1104 28-29 0.02666546756139354 1104 30-31 0.059749181134321816 1104 32-33 -0.10187848385522358 1104 34-35 -0.04879240985957267 1104 36-37 -0.014229871590949017 1104 38-39 -0.0363853027679113 1104 40-41 -0.0980245999555791 1104 42-43 -0.01270226612819414 1104 44-45 -0.0496668793174635 1104 46-47 -0.15072878151915603 1104 48-49 -0.1697541067272681 1104 50-51 -0.14705636436414693 1104 52-53 -0.2981517812059238 1104 54-55 -0.37691453824739796 1104 56-57 -0.3360340464798739 1104 58-59 -0.4141598594285867 1104 60-61 -0.368312169487929 1104 62-63 -0.4343637802466702 1104 64-65 -0.48189826780160416 1104 66-67 -0.5493269913438823 1104 68-69 -0.7918300566143444 1104 70-71 -0.6238762406948446 1104 72-73 -0.8406207076656358 1104 74-75 -1.06616319353218 1104 76-77 -0.9284148390517792 1104 78-79 -0.5873867875702494 1104 80-81 -0.3319673859448784 1104 82-83 -0.4729291190988789 1104 84-85 -0.9166464477739922 1104 86-87 -1.120726242213852 1104 88-89 -0.7848248143859422 1104 90-91 -0.9226279951930714 1104 92-93 -1.0317843081719893 1104 94-95 -0.9608800684322176 1104 96-97 -1.402014628097799 1104 98-99 -1.2852733956182512 1104 100-101 -1.1076264723587492 1105 1 -0.06173625347217637 1105 2 -0.03600409940661109 1105 3 -0.008042941677089743 1105 4 0.007864009899670066 1105 5 -0.062023908915769255 1105 6 -0.055994272870208306 1105 7 0.023142700172726904 1105 8 -0.05469699162304664 1105 9 0.06216819303234189 1105 10-11 -0.008714222864611543 1105 12-13 0.043443514833349184 1105 14-15 0.07601286325410683 1105 16-17 0.11154168950864118 1105 18-19 0.11114248435561791 1105 20-21 0.10126951899124492 1105 22-23 0.06371771565277129 1105 24-25 0.005764470149024703 1105 26-27 -0.0028853432788693567 1105 28-29 -0.0016317899333913033 1105 30-31 0.049616241133804806 1105 32-33 0.1602990386032701 1105 34-35 0.09057372995894752 1105 36-37 0.014410562834740404 1105 38-39 -0.062014799041229196 1105 40-41 0.14009970616285727 1105 42-43 0.027214311046421358 1105 44-45 0.003421813910307492 1105 46-47 -0.10760950779795309 1105 48-49 -0.09564143231861522 1105 50-51 -0.12540639503684758 1105 52-53 -0.1847555772441467 1105 54-55 -0.34695931260784363 1105 56-57 -0.39406840624167927 1105 58-59 -0.36792029772690427 1105 60-61 -0.4905566330585813 1105 62-63 -0.5509543246416868 1105 64-65 -0.3899516305177215 1105 66-67 -0.5678339094853797 1105 68-69 -0.5244364465054048 1105 70-71 -0.7372767667842162 1105 72-73 -0.8341922632252867 1105 74-75 -0.7443328185991369 1105 76-77 -0.785256464482945 1105 78-79 -0.7902488716483091 1105 80-81 -0.5561954755673639 1105 82-83 -0.5748045478899542 1105 84-85 -0.7630378890393033 1105 86-87 -0.49405764883875136 1105 88-89 -1.0384821267198845 1105 90-91 -1.17263411820716 1105 92-93 -1.1349086877665897 1105 94-95 -1.2440029093493976 1105 96-97 -1.4805528730200948 1105 98-99 -1.5163968635320728 1105 100-101 -1.9591873222964953 1106 1 -0.039400249933400744 1106 2 0.00639037679307819 1106 3 0.027345044471935864 1106 4 0.02003434089916567 1106 5 0.07659334397503414 1106 6 0.10851683071487628 1106 7 0.15924383161180344 1106 8 0.1141156539538386 1106 9 0.17088725470510013 1106 10-11 0.10160419740591209 1106 12-13 0.17080789074482539 1106 14-15 0.14218456276267943 1106 16-17 0.059922079741077994 1106 18-19 0.041909471493021044 1106 20-21 0.07177427767056344 1106 22-23 0.11885854076698621 1106 24-25 0.17709268424466273 1106 26-27 0.10531700253923049 1106 28-29 0.14550795696153784 1106 30-31 0.1285786624272589 1106 32-33 0.1693595363811724 1106 34-35 0.08409363208981091 1106 36-37 0.08569868929050983 1106 38-39 0.2230090116345238 1106 40-41 0.18367953240367285 1106 42-43 0.20009405503845556 1106 44-45 0.31128742355346617 1106 46-47 0.07660315021991693 1106 48-49 0.16576702617340544 1106 50-51 0.11950309674864457 1106 52-53 0.1857049467980687 1106 54-55 0.1151357503919499 1106 56-57 0.06851738476348856 1106 58-59 -0.003202673679844281 1106 60-61 0.03522041054709035 1106 62-63 0.0042442819672388055 1106 64-65 -0.02540665978860801 1106 66-67 -0.008797948107748255 1106 68-69 -0.39384722731586663 1106 70-71 -0.38258091447260867 1106 72-73 -0.449156979190537 1106 74-75 -0.6302037225754198 1106 76-77 -0.6878372790914362 1106 78-79 -0.5146087480677899 1106 80-81 -0.20512188710154433 1106 82-83 -0.2062256547235144 1106 84-85 -0.2713188895715035 1106 86-87 -0.15171971003460882 1106 88-89 -0.28986577234514854 1106 90-91 -0.34987470625137007 1106 92-93 -0.31563358288211774 1106 94-95 -0.6151016562650682 1106 96-97 -0.9062795997260658 1106 98-99 -1.035012319390745 1106 100-101 -1.2393872075629062 1107 1 -0.019109794887164355 1107 2 -0.008685241675856048 1107 3 -0.015329812804459664 1107 4 -0.03775753303666818 1107 5 -0.051726356889652436 1107 6 0.00824106534913227 1107 7 0.010039411353396588 1107 8 0.055660073544530064 1107 9 3.291624150847383E-5 1107 10-11 -0.023758871401881265 1107 12-13 -0.005113052065581769 1107 14-15 -0.05162640775779437 1107 16-17 -0.07117063217425112 1107 18-19 0.05115528994192431 1107 20-21 0.06511918469156086 1107 22-23 -0.03284379288907502 1107 24-25 -0.07985920705170457 1107 26-27 -0.11926951634034566 1107 28-29 -0.008917977001665633 1107 30-31 0.050255289142441484 1107 32-33 -0.05864433635277777 1107 34-35 -0.1734845737660251 1107 36-37 -0.10105656060098767 1107 38-39 -0.16463830249094258 1107 40-41 -0.035368976927614426 1107 42-43 -0.044895094629353594 1107 44-45 -0.10991980109849209 1107 46-47 -0.10316982744509318 1107 48-49 -0.183615673389248 1107 50-51 -0.4640925364099786 1107 52-53 -0.16114979229317328 1107 54-55 -0.33533331781490716 1107 56-57 -0.3871999056581714 1107 58-59 -0.4579798245495468 1107 60-61 -0.41234076863362645 1107 62-63 -0.47869565727089736 1107 64-65 -0.6448925209711334 1107 66-67 -0.7092386385227272 1107 68-69 -0.7197157546753985 1107 70-71 -0.93001055424158 1107 72-73 -1.1309204085632203 1107 74-75 -1.1055030248155937 1107 76-77 -0.9128063973767517 1107 78-79 -0.6908962651779298 1107 80-81 -0.4832335954446165 1107 82-83 -0.888217441856689 1107 84-85 -0.9788767301884675 1107 86-87 -0.7691997621162052 1107 88-89 -0.9584585677245734 1107 90-91 -0.5185088998212954 1107 92-93 -0.46652931953621035 1107 94-95 -0.6157934066740438 1107 96-97 -0.597872428491133 1107 98-99 -0.6178173806533884 1107 100-101 -1.3029047183297742 1108 1 -0.09443074430091514 1108 2 -0.08083355949163007 1108 3 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2316 64-65 0.13268435914010723 2316 66-67 0.09220314503994587 2316 68-69 0.1250941454474983 2316 70-71 0.4424923368833653 2316 72-73 0.6305114152422568 2316 74-75 0.5382399803185471 2316 76-77 0.4367250695546794 2316 78-79 0.5362650901358883 2316 80-81 0.5393521728698971 2316 82-83 0.6744528290963316 2316 84-85 0.6880528381771853 2316 86-87 0.6335345121829619 2316 88-89 0.49538499782709167 2316 90-91 0.18175282851752783 2316 92-93 0.30092598320624475 2316 94-95 0.6957569092531841 2316 96-97 0.7713560630404892 2316 98-99 1.1963175026197064 2316 100-101 1.9358606859048813 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 13.0 5 45.0 6 77.0 7 256.0 8 564.0 9 1117.0 10 1843.0 11 2969.0 12 4425.0 13 6318.0 14 8033.0 15 10898.0 16 14109.0 17 19333.0 18 19763.0 19 15821.0 20 15459.0 21 16565.0 22 18123.0 23 19725.0 24 22279.0 25 25617.0 26 29713.0 27 34869.0 28 42012.0 29 50487.0 30 60813.0 31 73728.0 32 92054.0 33 117968.0 34 159562.0 35 227692.0 36 328021.0 37 396768.0 38 177134.0 39 4714.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.622531746675136 19.40653996676751 15.180861428678712 22.790066857878642 2 15.633971505162236 20.75588986434143 39.79861230813733 23.811526322358997 3 20.621112966914527 23.798103117947864 29.258127725504878 26.32265618963273 4 13.617617980555671 16.787029717290476 36.36395607095377 33.23139623120008 5 14.361144627845373 36.9057383951197 33.47628303049395 15.256833946540974 6 32.39866223651389 35.69031924441723 16.851272876409435 15.05974564265944 7 29.01784741333365 32.28993923399568 20.179930377652695 18.51228297501798 8 25.80365864048201 35.12614840219289 19.108005777426428 19.962187179898677 9 25.71227187982319 18.207264182531805 20.753759983198705 35.326703954446295 10-11 25.494553448128976 26.421571832470487 26.50575166972775 21.57812304967279 12-13 26.692339164254452 23.599751745016572 27.16928394386624 22.538625146862735 14-15 22.803252204877317 25.252708622840476 26.314734952831436 25.62930421945077 16-17 22.401807765426387 28.564140107764537 26.65349412486143 22.380558001947648 18-19 22.05645472863333 27.340244054659685 28.902511872849068 21.700789343857917 20-21 22.901299326561304 25.993292360364002 28.83418231386325 22.27122599921145 22-23 22.397755798725242 26.029054550713955 28.97733013950226 22.595859511058542 24-25 22.868947077615157 26.11573039746336 28.89653995598077 22.118782568940713 26-27 22.534056989670216 26.08541020456484 29.315214516188153 22.065318289576794 28-29 21.877247519926772 27.069625319234508 29.092343721209456 21.96078343962926 30-31 22.31506192505592 26.152191400035267 28.859344630619173 22.673402044289638 32-33 22.216245346457363 26.355867475823374 29.011086279008484 22.41680089871078 34-35 23.054503284127158 26.26771667585598 28.63729652851982 22.040483511497047 36-37 22.758828093337943 25.969314095519884 29.012042295180407 22.259815515961765 38-39 22.26626768954097 26.54239916373338 29.081419572503275 22.10991357422238 40-41 22.544938195893728 26.500792334933525 28.745931202838236 22.20833826633451 42-43 22.306280136760336 26.67529922515027 28.117851265706783 22.900569372382616 44-45 22.65873463054486 26.28504062402867 28.229966304532233 22.826258440894236 46-47 23.023162504086628 26.85342137330467 27.8688613915335 22.254554731075203 48-49 22.986506745141416 26.764276322323372 27.869969811625435 22.379247120909778 50-51 22.248138752557427 26.958312663813427 28.274664918497905 22.51888366513124 52-53 22.945432445123366 27.2480152479677 27.402431630815315 22.40412067609362 54-55 22.36016091995015 26.628814220869668 27.797871307628146 23.21315355155204 56-57 22.804648690791133 26.99893159165106 27.57236522427412 22.624054493283687 58-59 23.453278176751862 26.529075712834754 27.482981100458503 22.53466500995488 60-61 22.82416000071328 26.683304925945134 28.41075646868674 22.08177860465485 62-63 22.47924514002797 26.860173131975916 28.103347203341283 22.557234524654824 64-65 22.211924751632687 27.151061664539615 27.760918490346782 22.876095093480917 66-67 22.512560997023797 26.865289792671415 27.413003006416776 23.20914620388801 68-69 22.217808167608148 26.789425818454887 27.80790012304315 23.184865890893818 70-71 22.639550109107013 26.781951166718137 27.658720301433043 22.91977842274181 72-73 22.7090899364602 26.400495915581416 28.05930183486784 22.831112313090546 74-75 22.243215585578625 27.453697374599535 27.34901123982858 22.954075799993266 76-77 22.37411693022525 26.7895215787685 27.438492186944192 23.397869304062066 78-79 22.534200242297903 26.890781904889526 27.576458710401496 22.998559142411075 80-81 22.638486095047515 26.936824039011633 27.498074432174196 22.92661543376666 82-83 22.52792620902951 26.738899356676832 27.792497072651734 22.94067736164193 84-85 22.315235287474515 26.93465029332921 27.569949259296685 23.180165159899587 86-87 22.397305459068328 26.830203114459728 27.588253479428122 23.184237947043822 88-89 22.13522841407594 26.940593963384018 27.225298711206836 23.698878911333207 90-91 22.394003093050387 26.5762110655009 27.471673770967552 23.55811207048116 92-93 22.68514115663443 26.642014530742603 27.50865326129382 23.164191051329144 94-95 22.3386887461043 26.458349431272204 27.666809319171108 23.53615250345239 96-97 21.887456557224596 26.440549182558843 28.037514646032243 23.63447961418432 98-99 22.06129759023344 26.649663911602843 27.245189617950672 24.043848880213048 100-101 22.41294336929209 25.17510885948545 28.18099774776894 24.230950023453516 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 726.0 1 521.5 2 447.0 3 565.0 4 455.0 5 436.5 6 861.5 7 1894.5 8 3038.0 9 3271.0 10 2612.5 11 2037.5 12 1998.0 13 2133.5 14 2309.0 15 2518.5 16 2666.5 17 2815.5 18 2938.5 19 3070.5 20 3411.0 21 4040.5 22 4832.0 23 5924.5 24 7291.5 25 9040.0 26 11293.5 27 13687.0 28 16059.5 29 18922.5 30 21926.5 31 24934.5 32 28260.5 33 31112.5 34 33913.0 35 36657.5 36 40212.0 37 44162.0 38 46977.5 39 48605.0 40 50890.5 41 56065.5 42 60912.0 43 64385.5 44 67905.5 45 71473.5 46 73558.0 47 81905.5 48 99731.5 49 99903.0 50 85429.5 51 77663.5 52 72598.5 53 70578.5 54 69235.5 55 64281.5 56 58131.5 57 52218.0 58 46310.5 59 40370.0 60 34076.5 61 27643.0 62 22575.5 63 18283.5 64 14617.0 65 11873.0 66 9004.0 67 7163.5 68 5527.0 69 3852.5 70 2964.0 71 2220.5 72 1743.0 73 1492.5 74 1202.0 75 1004.5 76 723.5 77 444.0 78 273.5 79 167.0 80 107.5 81 67.5 82 41.5 83 26.5 84 15.5 85 7.5 86 2.5 87 4.0 88 2.5 89 2.0 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40660916978223705 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.677478537732579E-4 16-17 0.002278477501520636 18-19 0.0 20-21 0.0 22-23 0.0 24-25 7.429817939741205E-5 26-27 0.0034672483718792284 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01203630506238075 36-37 0.025063252516726996 38-39 0.019664251480515055 40-41 0.008668120929698073 42-43 0.008816717288492896 44-45 0.003690142910071465 46-47 0.0055475973950067665 48-49 0.003392950192481817 50-51 8.668120929698071E-4 52-53 0.0 54-55 0.0 56-57 4.95321195982747E-5 58-59 0.0016593260065422024 60-61 0.0025261380995120096 62-63 0.0013621332889525541 64-65 0.002848096876900795 66-67 0.0021546472025249495 68-69 0.004358826524648173 70-71 1.2383029899568676E-4 72-73 0.0 74-75 0.0 76-77 0.0917087194362056 78-79 0.014983466178478094 80-81 3.467248371879229E-4 82-83 0.0 84-85 0.0 86-87 0.10270484998702258 88-89 0.40678253220083094 90-91 0.40891241334355677 92-93 0.0 94-95 0.0 96-97 2.476605979913735E-5 98-99 1.7336241859396145E-4 100-101 2.476605979913735E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2018892.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.04746795262046 #Duplication Level Percentage of deduplicated Percentage of total 1 78.5108642456451 44.00335147740328 2 12.77595715959581 14.32120098932996 3 3.8516248651962326 6.4762146359280575 4 1.5617138216381607 3.501204214777169 5 0.8478133152090579 2.3758894806992283 6 0.5397998581953651 1.8152649151820275 7 0.33471226704665613 1.313184254245248 8 0.23899031648753635 1.071584168345745 9 0.17832848381883956 0.8995373983688225 >10 1.013326582062894 10.566969378437335 >50 0.08850814322155104 3.4119921623244407 >100 0.05297685113998377 5.417747012694727 >500 0.003354023741431198 1.2578144387529138 >1k 0.0016770118707155977 2.036664062119776 >5k 2.647913480077259E-4 0.9535268350099265 >10k+ 8.826378266924198E-5 0.5778545763813724 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11681 0.5785846890274468 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8052 0.39883262700530786 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6126 0.3034337646590308 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5097 0.2524652135924061 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4399 0.2178917941128104 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3794 0.1879248617558542 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3713 0.18391276006839397 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3506 0.17365961131155108 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2680 0.1327460805233762 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2598 0.12868444671631768 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2557 0.1266536298127884 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2428 0.12026398638461096 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2059 0.1019866342528476 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.476605979913735E-5 0.0 22-23 0.0 0.0 0.0 4.95321195982747E-5 0.0 24-25 0.0 0.0 0.0 1.981284783930988E-4 0.0 26-27 0.0 0.0 0.0 3.7149089698706024E-4 0.0 28-29 0.0 0.0 0.0 0.0013373672291534168 0.0 30-31 0.0 0.0 0.0 0.004507422883442997 0.0 32-33 0.0 0.0 0.0 0.010426511175436824 0.0 34-35 0.0 0.0 0.0 0.019168930284532308 0.0 36-37 0.0 0.0 0.0 0.03430099282180522 0.0 38-39 0.0 0.0 0.0 0.06114740164407011 0.0 40-41 0.0 0.0 0.0 0.09051994856584701 0.0 42-43 0.0 0.0 0.0 0.12063547728159804 0.0 44-45 0.0 0.0 0.0 0.16090509051499535 0.0 46-47 0.0 0.0 0.0 0.21350820152836308 0.0 48-49 0.0 0.0 0.0 0.27183227235533153 0.0 50-51 0.0 0.0 0.0 0.3345399357667473 0.0 52-53 0.0 0.0 0.0 0.39917935184249576 0.0 54-55 0.0 0.0 0.0 0.4715705446353743 0.0 56-57 0.0 0.0 0.0 0.5619171307826274 0.0 58-59 0.0 0.0 0.0 0.664646746829449 0.0 60-61 0.0 0.0 0.0 0.7779762364703016 0.0 62-63 0.0 0.0 0.0 0.8972248144031478 0.0 64-65 0.0 0.0 0.0 1.0183308468209296 0.0 66-67 0.0 0.0 0.0 1.1683388710243046 0.0 68-69 0.0 0.0 0.0 1.3396704727147366 0.0 70-71 0.0 0.0 0.0 1.528164953845971 0.0 72-73 0.0 0.0 0.0 1.7246093401727283 2.476605979913735E-5 74-75 0.0 0.0 0.0 1.9308363201201453 4.95321195982747E-5 76-77 0.0 0.0 0.0 2.1549939273621375 4.95321195982747E-5 78-79 0.0 0.0 0.0 2.4305411086873394 4.95321195982747E-5 80-81 0.0 0.0 0.0 2.734816919379541 4.95321195982747E-5 82-83 0.0 0.0 0.0 3.041049248795874 4.95321195982747E-5 84-85 0.0 0.0 0.0 3.352829175607214 7.429817939741205E-5 86-87 0.0 0.0 0.0 3.680929935826186 9.90642391965494E-5 88-89 0.0 2.476605979913735E-5 0.0 4.049027882620765 9.90642391965494E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 410 0.0 28.934021 94-95 GTACATG 6670 0.0 21.638592 1 GAGTACT 7740 0.0 21.120863 12-13 TACATGG 6760 0.0 20.847902 2 GTACTTT 8115 0.0 20.203327 14-15 AGTACTT 7920 0.0 20.071646 12-13 ACATGGG 6840 0.0 19.77158 3 GTGTAGC 970 0.0 19.642666 1 AGAGTAC 11660 0.0 19.127697 10-11 ATTCGCG 340 0.0 18.864044 80-81 CTAGGAC 900 0.0 18.453474 3 TAGGACC 650 0.0 18.250689 4 ACTTTTT 9370 0.0 17.547758 16-17 ATGGGGA 2580 0.0 17.288637 5 CATGGGG 4065 0.0 17.27642 4 CGATCGT 385 0.0 17.256052 24-25 TACTTTT 9380 0.0 16.770426 14-15 CGCGATC 415 0.0 16.580362 22-23 TAAGGTG 790 0.0 16.217701 5 AATTCGC 420 0.0 15.819615 78-79 >>END_MODULE