FastQCFastQC Report
Fri 27 May 2016
SRR939069_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939069_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2931037
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94210.32142207689633395No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69030.23551391538216682No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46580.15891986351588192No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32910.11228108004095477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA128600.029.3473431
GGTATCA88600.029.1479361
ATCAACG149650.024.7911153
GGACCGT3450.024.7116246
TCAACGC151600.024.4722334
TATCAAC152800.024.4660972
CAACGCA152150.024.3837685
AACGCAG156350.023.7804266
GTACATG90250.023.0919151
TACATGG87550.022.7843652
GAGTACT96050.021.81970812-13
ACATGGG90050.021.7834993
ACGCAGA172850.021.4555787
CGCAGAG174650.021.2615728
AGAGTAC137000.020.62127510-11
GTGTAGC16650.020.5286751
GTACTTT101900.020.3592914-15
AGTACTT102100.019.99330512-13
GCAGAGT190650.019.2526519
CATGGGG60500.018.7960644
Produced by FastQC (version 0.11.5)