FastQCFastQC Report
Fri 27 May 2016
SRR939069_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939069_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2931037
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97250.3317938326947084No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73960.25233390093676744No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50030.17069044164232658No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35510.12115166065798555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA126850.028.8960481
GGTATCA87500.028.1812271
ATCAACG148850.024.3205953
TATCAAC151250.024.0601582
TCAACGC151600.023.8794254
CAACGCA153450.023.5915345
AACGCAG158150.022.9204256
GTACATG91900.021.1340541
TACATGG88600.021.0454062
GAGTACT99650.021.02200512-13
ACGCAGA177450.020.3205817
GTACTTT102750.020.31849914-15
CGCAGAG178500.020.1478868
ACATGGG90000.019.71643
AGAGTAC142550.019.67165810-11
AGTACTT105500.019.40658212-13
GCAGAGT192950.018.4183359
TACTTTT119900.016.93734614-15
ACTTTTT124900.016.92339516-17
GTGTAAG13000.016.4781071
Produced by FastQC (version 0.11.5)