##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939069_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2931037 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.42659713951069 34.0 31.0 34.0 31.0 34.0 2 32.65205591058728 34.0 31.0 34.0 31.0 34.0 3 32.74243962119891 34.0 31.0 34.0 31.0 34.0 4 36.223439690457674 37.0 35.0 37.0 35.0 37.0 5 36.1782532939707 37.0 35.0 37.0 35.0 37.0 6 36.21090590122199 37.0 37.0 37.0 35.0 37.0 7 36.16555574016978 37.0 37.0 37.0 35.0 37.0 8 36.14758974383469 37.0 36.0 37.0 35.0 37.0 9 37.88106086685361 39.0 38.0 39.0 35.0 39.0 10-11 37.81339027791188 39.0 38.0 39.0 35.0 39.0 12-13 37.74338911450111 39.0 38.0 39.0 35.0 39.0 14-15 39.08725768388457 40.0 38.0 41.0 36.0 41.0 16-17 39.01831399603621 40.0 38.0 41.0 36.0 41.0 18-19 39.0414186173699 40.0 38.5 41.0 35.5 41.0 20-21 39.11015623480699 40.0 39.0 41.0 36.0 41.0 22-23 39.069845416485705 40.0 39.0 41.0 35.0 41.0 24-25 39.01287479482518 40.0 38.5 41.0 35.0 41.0 26-27 38.86181614220496 40.0 38.0 41.0 35.0 41.0 28-29 38.697905553563466 40.0 38.0 41.0 34.5 41.0 30-31 38.49226638899475 40.0 38.0 41.0 34.0 41.0 32-33 38.341580471348536 40.0 38.0 41.0 34.0 41.0 34-35 38.28757535302352 40.0 38.0 41.0 34.0 41.0 36-37 38.167413444456685 40.0 38.0 41.0 34.0 41.0 38-39 38.021981298769006 40.0 38.0 41.0 33.0 41.0 40-41 37.83666088145595 40.0 37.5 41.0 33.0 41.0 42-43 37.66051179156047 40.0 37.0 41.0 33.0 41.0 44-45 37.43484814418924 40.0 37.0 41.0 32.5 41.0 46-47 37.15377595028654 40.0 37.0 41.0 31.5 41.0 48-49 36.83077934533068 40.0 36.0 41.0 31.0 41.0 50-51 36.55565845808156 39.5 36.0 41.0 30.5 41.0 52-53 36.29640686896822 39.0 35.0 41.0 30.0 41.0 54-55 36.032710948377655 39.0 35.0 40.5 29.5 41.0 56-57 35.73005219654341 38.5 35.0 40.0 28.5 41.0 58-59 35.401852484291396 38.0 35.0 40.0 28.0 41.0 60-61 35.020992399618294 38.0 34.0 40.0 27.5 41.0 62-63 34.60048013723471 37.0 34.0 40.0 26.5 41.0 64-65 34.165223093396634 37.0 34.0 39.5 26.0 41.0 66-67 33.785544501826486 36.0 33.0 39.0 26.0 40.5 68-69 33.289269463333284 35.5 33.0 39.0 25.0 40.0 70-71 32.818443097101806 35.0 32.0 38.0 23.5 40.0 72-73 32.316563216363356 35.0 32.0 37.5 22.0 39.5 74-75 31.798755866950845 35.0 31.0 37.0 20.5 39.0 76-77 29.84430749253592 33.0 28.5 35.0 18.0 37.5 78-79 30.82637578440668 34.0 30.5 36.0 18.5 38.0 80-81 30.858447198039464 34.0 31.0 35.5 18.0 37.0 82-83 30.54703608313372 34.0 31.0 35.0 17.5 37.0 84-85 30.13685651187617 34.0 30.5 35.0 10.5 36.5 86-87 29.54639347780325 34.0 29.5 35.0 4.5 36.0 88-89 28.959059199866804 34.0 29.0 35.0 2.0 35.5 90-91 28.4555305852502 34.0 29.0 35.0 2.0 35.0 92-93 28.121698736658733 34.0 28.5 35.0 2.0 35.0 94-95 27.726004994136886 33.0 27.0 35.0 2.0 35.0 96-97 27.090139940232756 33.0 26.5 35.0 2.0 35.0 98-99 26.206918916410814 33.0 25.0 35.0 2.0 35.0 100-101 22.505972630164685 28.5 9.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12144326067709699 1101 2 0.1113863431806621 1101 3 0.17182186172018987 1101 4 0.10627460930406585 1101 5 0.08705178562090055 1101 6 0.16275427037049184 1101 7 0.11864628874874228 1101 8 0.18729895857141088 1101 9 0.23019228682352377 1101 10-11 0.22504687389518807 1101 12-13 0.2275329038527687 1101 14-15 0.2789509024134631 1101 16-17 0.3321313829278836 1101 18-19 0.2845955243172611 1101 20-21 0.29396402517146925 1101 22-23 0.25807906638377176 1101 24-25 0.20715830166571436 1101 26-27 0.2310269140494441 1101 28-29 0.20647893990819455 1101 30-31 0.18064293951081112 1101 32-33 0.17017878283364496 1101 34-35 0.2875803476037717 1101 36-37 0.30142697914131844 1101 38-39 0.3845336826677581 1101 40-41 0.4250047420033525 1101 42-43 0.4162795421594865 1101 44-45 0.5034275676122704 1101 46-47 0.31557429510263546 1101 48-49 0.27810014786293635 1101 50-51 0.2679647596979322 1101 52-53 0.3209567283372081 1101 54-55 0.2682540420294046 1101 56-57 0.2596646811688217 1101 58-59 0.33187736410998525 1101 60-61 0.20284991911415062 1101 62-63 0.11424464478301388 1101 64-65 7.523972535850021E-4 1101 66-67 0.05557844734150308 1101 68-69 0.09591657909275142 1101 70-71 -0.008872444523113643 1101 72-73 -0.016260628309112235 1101 74-75 0.03883610998948228 1101 76-77 0.09095001089302102 1101 78-79 0.18511263235814468 1101 80-81 0.10097100356705724 1101 82-83 0.07509999666445921 1101 84-85 0.12655850897436238 1101 86-87 0.17790222838234726 1101 88-89 0.3671015721075115 1101 90-91 0.23557778031299748 1101 92-93 0.18959090814610846 1101 94-95 -0.03423807873306828 1101 96-97 -0.020292227247438177 1101 98-99 -0.04747023998692512 1101 100-101 -0.46091919221020206 1102 1 0.0521616150613724 1102 2 0.08493936388197199 1102 3 0.12028419582563288 1102 4 0.04282167164108586 1102 5 0.05452406975766877 1102 6 0.09478375626126478 1102 7 0.10348378128257707 1102 8 0.03108492363703874 1102 9 0.11673279350301158 1102 10-11 0.08435517918486823 1102 12-13 0.10501567704159243 1102 14-15 0.16248090547276917 1102 16-17 0.16405962033010013 1102 18-19 0.11314614127747546 1102 20-21 0.207120501432712 1102 22-23 0.17556810386756183 1102 24-25 0.1255447358250592 1102 26-27 0.14732573548790384 1102 28-29 0.03195641029847707 1102 30-31 0.11437325432805778 1102 32-33 0.14142129845796347 1102 34-35 0.055247844653727896 1102 36-37 0.16800332341531288 1102 38-39 0.20325372695486266 1102 40-41 0.2550981237028722 1102 42-43 0.2128050436672808 1102 44-45 0.1701903839227441 1102 46-47 0.24527943619494863 1102 48-49 0.1679658297241602 1102 50-51 0.16019557711558718 1102 52-53 0.19255397787467388 1102 54-55 0.024921145739916994 1102 56-57 -0.024452417442830665 1102 58-59 0.041354659682731665 1102 60-61 0.04668468740807441 1102 62-63 0.15579658744286462 1102 64-65 0.22734724304995524 1102 66-67 0.30012195505533157 1102 68-69 0.20680916828612084 1102 70-71 0.3312001047535773 1102 72-73 0.07762440002589699 1102 74-75 0.07152334685267192 1102 76-77 0.15503811893025699 1102 78-79 0.11737083785615354 1102 80-81 0.15374258989050205 1102 82-83 0.135935770159211 1102 84-85 0.19626772914248036 1102 86-87 0.19306983469326156 1102 88-89 0.26030991090389577 1102 90-91 0.42883492233767484 1102 92-93 0.4185144691792786 1102 94-95 0.28121433447730837 1102 96-97 0.23241161728149962 1102 98-99 0.11515158598221831 1102 100-101 -0.010976007908819696 1103 1 0.059043971002509466 1103 2 0.061253870917042263 1103 3 0.08962624815091402 1103 4 0.06884519931954003 1103 5 0.11330841637159494 1103 6 0.06337980211529981 1103 7 0.1321536061282984 1103 8 0.13315634532761322 1103 9 0.18851034587645898 1103 10-11 0.19961062902705606 1103 12-13 0.15992294330894907 1103 14-15 0.22626105881067815 1103 16-17 0.1717899813484962 1103 18-19 0.18676618513688226 1103 20-21 0.20991993817374066 1103 22-23 0.24302162366946334 1103 24-25 0.1273588943648889 1103 26-27 0.14537260384737039 1103 28-29 0.2199183081525362 1103 30-31 0.2560824676354727 1103 32-33 0.26617763512136605 1103 34-35 0.23671313962802998 1103 36-37 0.256663345061682 1103 38-39 0.2817262128308897 1103 40-41 0.23194911507265914 1103 42-43 0.19705065064399463 1103 44-45 0.26448157860975385 1103 46-47 0.24575712966647245 1103 48-49 0.42185310993132674 1103 50-51 0.28249459736230875 1103 52-53 0.365913075300341 1103 54-55 0.34658026639859685 1103 56-57 0.3881320855689623 1103 58-59 0.3741178869186257 1103 60-61 0.3631353625821845 1103 62-63 0.3432654920344973 1103 64-65 0.32420412767253737 1103 66-67 0.3981465820520924 1103 68-69 0.34241277337017806 1103 70-71 0.18302247984778575 1103 72-73 0.15108110445093814 1103 74-75 0.09905574557655683 1103 76-77 0.1859728794885278 1103 78-79 0.3291903513228043 1103 80-81 0.2998235767836803 1103 82-83 0.3321949712834602 1103 84-85 0.4371734200980377 1103 86-87 0.5345512511139034 1103 88-89 0.5242961864247953 1103 90-91 0.7296669193003851 1103 92-93 0.7351032674656999 1103 94-95 0.5429479991172279 1103 96-97 0.5407402284571461 1103 98-99 0.18528795245187624 1103 100-101 -0.17029060560931342 1104 1 0.07154516008200318 1104 2 0.09076190362738856 1104 3 0.14007000715101725 1104 4 0.03000494271891796 1104 5 0.07854929927542287 1104 6 0.08901170902705502 1104 7 0.08963349018348055 1104 8 0.04397948355714476 1104 9 0.10513318516867542 1104 10-11 0.10860846189550699 1104 12-13 0.14687341090421313 1104 14-15 0.1313598043454718 1104 16-17 0.092341703299617 1104 18-19 0.11398331269257511 1104 20-21 0.10239721992161321 1104 22-23 0.15513621157005275 1104 24-25 0.06888488518246305 1104 26-27 0.0738817277773407 1104 28-29 0.10990275991308351 1104 30-31 0.153321671067026 1104 32-33 0.08867963878864771 1104 34-35 0.24601761833012148 1104 36-37 0.1607650406070391 1104 38-39 0.24409184684807173 1104 40-41 0.23536587349434512 1104 42-43 0.2407843110516339 1104 44-45 0.27246645242517786 1104 46-47 0.20839242504474953 1104 48-49 0.19435514093377293 1104 50-51 0.16245811585672953 1104 52-53 0.022513751244197522 1104 54-55 0.12320635310791062 1104 56-57 0.13522512789581498 1104 58-59 0.14851957491947587 1104 60-61 0.2302730869628533 1104 62-63 0.2606985454922395 1104 64-65 0.1238550303342123 1104 66-67 0.23953540503286774 1104 68-69 0.04256205068319474 1104 70-71 0.014482899081677658 1104 72-73 0.06694634976213365 1104 74-75 0.02757826373530392 1104 76-77 0.03067855087834559 1104 78-79 0.2783872716342479 1104 80-81 0.3554749572639686 1104 82-83 0.4177958473206935 1104 84-85 0.1937795084037255 1104 86-87 0.2741849380913237 1104 88-89 0.39732901157927003 1104 90-91 0.45389929331536294 1104 92-93 0.5571624415283516 1104 94-95 0.3323837521954367 1104 96-97 0.2538253239060637 1104 98-99 0.2300915110524464 1104 100-101 -0.13570520345989578 1105 1 -0.08905708876365992 1105 2 0.027191838654502476 1105 3 -0.04737831949177718 1105 4 -0.01644566958557192 1105 5 0.02631179993002064 1105 6 0.022288478272550094 1105 7 0.00898708326376152 1105 8 0.022154085682210223 1105 9 -0.07105768900567 1105 10-11 0.0282707065835055 1105 12-13 0.03153199779294624 1105 14-15 0.0530706017562963 1105 16-17 0.016797701534990495 1105 18-19 -0.06352177728187769 1105 20-21 0.011387445160593757 1105 22-23 0.031535657002187634 1105 24-25 0.027245482300337187 1105 26-27 0.004098429241146562 1105 28-29 -0.009870329581076476 1105 30-31 -0.014047770889817457 1105 32-33 -0.053571724753226135 1105 34-35 0.033008336565316654 1105 36-37 -0.0800490696643692 1105 38-39 0.035080357179573696 1105 40-41 0.0702801096067418 1105 42-43 0.16320418726302677 1105 44-45 0.09474223832305029 1105 46-47 0.049864516009961335 1105 48-49 -0.05331899596577472 1105 50-51 -0.006170132721273092 1105 52-53 -0.19030272244689428 1105 54-55 -0.15067791232506522 1105 56-57 -0.12839204149123162 1105 58-59 -0.04653382683383711 1105 60-61 -0.005246471287136956 1105 62-63 -0.17569436192128052 1105 64-65 -0.2675805355350036 1105 66-67 -0.227181002061414 1105 68-69 -0.2541160169620227 1105 70-71 -0.21189890533740652 1105 72-73 -0.3691717598618176 1105 74-75 -0.26641516968190615 1105 76-77 -0.20464330636327688 1105 78-79 -0.09301265251204072 1105 80-81 -0.1489309320308685 1105 82-83 -0.18579247226569962 1105 84-85 -0.04847788534950226 1105 86-87 -0.06488630885588265 1105 88-89 0.04086906831615167 1105 90-91 0.08005634011985308 1105 92-93 -0.0362686746796399 1105 94-95 -0.21464660674495661 1105 96-97 -0.278471350855547 1105 98-99 -0.4012545590746832 1105 100-101 -0.7480948545592732 1106 1 0.08481835400124282 1106 2 0.0666563883864697 1106 3 0.07902279373098509 1106 4 0.0734195878497701 1106 5 0.06875779200386489 1106 6 0.06299711825418797 1106 7 0.0903011822156401 1106 8 0.09711624835621535 1106 9 0.15880567463354822 1106 10-11 0.13554512227278082 1106 12-13 0.17335198217941894 1106 14-15 0.04928120835257488 1106 16-17 0.024930861133107385 1106 18-19 0.09050058392164573 1106 20-21 0.12011488621489974 1106 22-23 0.18857985810069522 1106 24-25 0.18589516449767984 1106 26-27 0.2147930252836261 1106 28-29 0.21502825197593012 1106 30-31 0.2220858333387028 1106 32-33 0.21422983011514418 1106 34-35 0.04310103479765104 1106 36-37 0.1420478000656189 1106 38-39 0.25992400321054987 1106 40-41 0.1715421085805886 1106 42-43 0.08026093476082963 1106 44-45 0.08805554270395533 1106 46-47 0.08070470277531427 1106 48-49 0.13506971803859358 1106 50-51 0.01212738573680383 1106 52-53 0.09425699825934686 1106 54-55 0.10306169173775004 1106 56-57 0.1327951216587877 1106 58-59 0.09061496533078639 1106 60-61 0.1227072459824825 1106 62-63 0.2985428061948099 1106 64-65 0.28136148599972444 1106 66-67 0.2908155932225256 1106 68-69 0.04981240063237635 1106 70-71 0.17744151840977196 1106 72-73 0.0769974714061945 1106 74-75 0.17362817148907084 1106 76-77 0.21132708145838208 1106 78-79 0.24912232986484995 1106 80-81 0.23087000838299687 1106 82-83 0.17391570301542458 1106 84-85 0.17574423017514462 1106 86-87 0.23380933906683765 1106 88-89 0.3881665749380083 1106 90-91 0.5327784661941841 1106 92-93 0.5673075637369429 1106 94-95 0.4308020814152691 1106 96-97 0.5290534782010496 1106 98-99 0.5260043717436105 1106 100-101 0.21711989317215696 1107 1 -0.06602703371157759 1107 2 -0.03137033755070462 1107 3 -0.024016927745840633 1107 4 -0.008532122351205373 1107 5 0.022385700941725872 1107 6 0.02076387316403583 1107 7 0.01711129675106804 1107 8 0.04205913122770255 1107 9 0.04697777593550967 1107 10-11 0.06561503917962597 1107 12-13 0.0368762279713053 1107 14-15 0.0836456014774356 1107 16-17 0.07102512171250197 1107 18-19 0.041849755851281145 1107 20-21 0.090988614142816 1107 22-23 0.12044023811859006 1107 24-25 0.03140899277585163 1107 26-27 0.031670737707237606 1107 28-29 -0.027832859888107464 1107 30-31 0.13252954894870328 1107 32-33 0.10059490388704262 1107 34-35 0.08986464849130726 1107 36-37 0.14007701066564238 1107 38-39 0.2032518552270446 1107 40-41 0.22634210075013783 1107 42-43 0.2763309510541916 1107 44-45 0.15422173803760586 1107 46-47 0.07405070035991912 1107 48-49 0.1710702240063 1107 50-51 0.24494594210251108 1107 52-53 0.24837197640938058 1107 54-55 0.06484456533316063 1107 56-57 0.19548843595809728 1107 58-59 0.09814876427488173 1107 60-61 0.17454107775424177 1107 62-63 0.10139120863188822 1107 64-65 0.1699507094898749 1107 66-67 0.2680075176164962 1107 68-69 0.09689262060786064 1107 70-71 0.12216567565730685 1107 72-73 0.04614661743509885 1107 74-75 0.07184207536122855 1107 76-77 -0.022110758300442512 1107 78-79 0.15361377208364146 1107 80-81 0.1918762366045499 1107 82-83 0.10429394399459468 1107 84-85 0.264699301447326 1107 86-87 0.14304724026459326 1107 88-89 0.10427238543502781 1107 90-91 0.21527732927269483 1107 92-93 0.23818686333307326 1107 94-95 0.2679019377565801 1107 96-97 0.36409595840656905 1107 98-99 0.3179609157432246 1107 100-101 -0.06628075193667016 1108 1 0.06861498142857414 1108 2 0.05269044275814139 1108 3 0.0712717599297008 1108 4 0.09109305996225459 1108 5 0.08201069534041494 1108 6 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92-93 -0.3382583110664079 2316 94-95 -3.8815642899692193E-4 2316 96-97 -0.15390075837918715 2316 98-99 -0.18553294238185813 2316 100-101 0.1166644266192769 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 8.0 4 17.0 5 45.0 6 117.0 7 252.0 8 702.0 9 1390.0 10 2428.0 11 3773.0 12 5387.0 13 7915.0 14 9818.0 15 13078.0 16 16993.0 17 23080.0 18 23379.0 19 17267.0 20 16232.0 21 16910.0 22 18014.0 23 19697.0 24 22716.0 25 26462.0 26 31047.0 27 37343.0 28 45035.0 29 54668.0 30 66313.0 31 81623.0 32 101589.0 33 131907.0 34 181255.0 35 265307.0 36 421396.0 37 674918.0 38 547195.0 39 45758.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.083605754913 19.390414639907167 15.290535475280095 23.235444129899737 2 15.611573651236746 20.325468426362413 40.1535361034337 23.909421818967143 3 19.657923117313086 23.889633600667615 29.498842900993743 26.953600381025556 4 13.609620076443935 16.89429372607715 36.34952407629109 33.14656212118783 5 14.187743109350034 36.78397782081905 34.00755432292393 15.020724746906982 6 32.47703799030855 35.894667996343955 17.050450062554653 14.577843950792843 7 28.710077696050917 32.177314718306185 20.546072942784413 18.566534642858485 8 25.836453105163805 35.65942702190385 19.422648025255228 19.08147184767712 9 25.815846064038084 17.492648506313635 20.365113098196986 36.32639233145129 10-11 25.02213380452038 26.541886028733174 26.9728085998232 21.463171566923243 12-13 25.992097677374936 23.66157438476553 27.558232802929478 22.78809513493006 14-15 22.599534971792952 25.273225395384856 25.982418999626404 26.14482063319578 16-17 22.198930836696963 28.298299871015992 26.79162131073068 22.711147981556362 18-19 22.162450041137323 26.95507203680336 29.009942387274947 21.87253553478437 20-21 22.703824619068268 26.107039931601 29.25345193527069 21.93568351406004 22-23 22.374606666514275 25.973111223092715 29.171740240740736 22.480541869652278 24-25 22.45497771168779 26.156760077249547 29.188315127785696 22.199947083276967 26-27 22.185041099489165 26.36699621886406 29.10440899386642 22.343553687780354 28-29 22.045115773018217 26.885791615731907 28.922408690166655 22.14668392108322 30-31 22.30326762508632 26.36050394822678 28.863543932723196 22.472684493963705 32-33 22.073757513125898 26.36757911960852 28.907123997411155 22.651539369854422 34-35 22.60777660059064 26.556369350141516 28.698269876122378 22.13758417314546 36-37 22.69619192189454 26.166800673812503 28.85611250047778 22.280894903815177 38-39 22.277662481830436 26.559920611019415 28.75577804459931 22.40663886255083 40-41 22.58863312200149 26.47598460394046 28.67792480771987 22.25745746633818 42-43 22.429092412912066 26.556701502295006 28.34143601768305 22.67277006710988 44-45 22.452821433677055 26.636446544946857 28.509003428594042 22.401728592782042 46-47 22.83433686023454 26.866890981768655 27.965118342024347 22.333653815972454 48-49 22.65811489181458 26.937169599757354 27.86603045810667 22.5386850503214 50-51 22.6384481787738 27.150372044536507 27.850534632238084 22.360645144451606 52-53 22.945931063282742 27.00151072862974 27.67538508568301 22.3771731224045 54-55 22.61206528610864 26.990055055599775 27.816230228414042 22.581649429877547 56-57 22.67726405364381 27.12321611770851 27.656645071351882 22.5428747572958 58-59 22.70269753870321 26.907830852975234 27.676128853517053 22.713342754804508 60-61 22.471146403394002 27.036164220806597 27.9899293701284 22.502760005671 62-63 22.43764430358455 27.050360522258277 28.015938519635863 22.49605665452131 64-65 22.31283316638053 27.108040756500923 27.854040030460926 22.725086046657623 66-67 22.409179960053308 27.057879031262004 27.633434849155858 22.89950615952883 68-69 22.535501999111883 26.911217323259386 27.810864584969973 22.742416092658754 70-71 22.522078378456754 26.939545245962606 27.619561008312765 22.918815367267875 72-73 22.486290688244466 26.929479225270782 27.72121266295854 22.863017423526212 74-75 22.37119490473849 27.32671064882497 27.426112328162354 22.87598211827418 76-77 22.43448104791346 26.976731782027624 27.58450326323299 23.004283906825922 78-79 22.39006272041149 27.140587579731108 27.61006522124439 22.859284478613016 80-81 22.617214541873924 27.043268533628755 27.458427003861235 22.881089920636086 82-83 22.54115209840134 27.115554089246157 27.539519559251957 22.803774253100546 84-85 22.395043119551204 27.255899533168638 27.47027758434984 22.87877976293032 86-87 22.474965162982596 27.12495559987978 27.443646001256866 22.95643323588076 88-89 22.414354449019775 27.12785480174618 27.151250007921146 23.306540741312904 90-91 22.359274589412284 27.10145203015883 27.307355944747748 23.23191743568114 92-93 22.449733660816975 26.966036252698277 27.36976708243533 23.21446300404942 94-95 22.24661442349585 26.81030638644275 27.521402834559915 23.421676355501482 96-97 22.194158586193215 26.567098948256195 27.7037785602843 23.53496390526629 98-99 22.067022189984286 26.754764482719036 27.36171071529037 23.816502612006307 100-101 22.283291331380312 26.057155295546984 27.97304482799386 23.686508545078848 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 717.0 1 483.0 2 358.5 3 480.0 4 419.5 5 403.0 6 697.5 7 1632.5 8 2803.5 9 3144.5 10 2623.5 11 2215.5 12 2281.0 13 2452.0 14 2622.0 15 2824.0 16 3065.0 17 3296.0 18 3639.5 19 4198.5 20 4884.5 21 5752.5 22 7073.5 23 8788.5 24 10979.5 25 13881.5 26 17691.5 27 22178.0 28 25929.5 29 29786.0 30 34315.0 31 39869.0 32 47274.0 33 51501.5 34 54050.5 35 58666.0 36 64118.0 37 69346.0 38 73454.5 39 76926.5 40 81164.0 41 86626.5 42 90056.5 43 93548.0 44 98017.0 45 103191.5 46 106835.5 47 110762.5 48 117547.0 49 117034.0 50 110343.5 51 106325.0 52 105454.0 53 104881.0 54 102860.0 55 95573.0 56 86832.5 57 78626.0 58 71166.0 59 63984.5 60 53453.5 61 44560.5 62 37212.5 63 27933.5 64 19609.0 65 14407.5 66 10737.5 67 8390.0 68 6716.0 69 5393.5 70 4158.5 71 2989.5 72 2207.0 73 1750.0 74 1318.0 75 964.5 76 711.5 77 442.5 78 287.5 79 194.5 80 123.0 81 71.5 82 42.5 83 27.5 84 17.0 85 10.0 86 5.0 87 4.5 88 3.0 89 1.5 90 1.5 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3311797155750678 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0010917637682499402 16-17 0.001825292550042869 18-19 1.7058808878905315E-5 20-21 0.0 22-23 0.0 24-25 5.629406930038755E-4 26-27 0.00134764590143352 28-29 0.0 30-31 0.003445879393538874 32-33 0.0 34-35 0.010985872918015024 36-37 0.028829387005349986 38-39 0.022364098440244867 40-41 0.014227046605007034 42-43 0.010951755300257212 44-45 0.0033435265402654416 46-47 0.0013988223280702358 48-49 9.382344883397924E-4 50-51 1.194116621523372E-4 52-53 3.411761775781063E-5 54-55 0.0 56-57 0.0 58-59 0.00487881933936692 60-61 0.011480578375503278 62-63 0.005117642663671595 64-65 0.002354115625288934 66-67 0.004469407926273193 68-69 0.0037699967622380746 70-71 6.823523551562126E-5 72-73 0.0 74-75 0.0 76-77 0.2133545226484688 78-79 4.6058783973044353E-4 80-81 0.0036847027178435483 82-83 6.823523551562126E-4 84-85 0.0 86-87 0.10634461455109574 88-89 0.3969755414210056 90-91 0.38308967099357666 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0017911749322850584 100-101 7.676463995507391E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2931037.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.453371409724426 #Duplication Level Percentage of deduplicated Percentage of total 1 73.75727608242347 40.1633234868579 2 14.630679367522445 15.933796351525833 3 4.958438147206476 8.100110221259401 4 2.292650630141064 4.993702251032404 5 1.1986108118328749 3.2634199856223423 6 0.7399809918275192 2.4176675870472097 7 0.4840906999841645 1.8452259477561832 8 0.3352151511317693 1.460287610139611 9 0.24779887025046676 1.2144135524992903 >10 1.2503485503727225 12.00270762182476 >50 0.07047073732552267 2.627894129704789 >100 0.029935293781410074 2.9245938250931074 >500 0.0028779811836011945 1.1139766344981146 >1k 0.0014389905916007207 1.1851460389117126 >5k 1.8769442499139835E-4 0.7537347562274046 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9725 0.3317938326947084 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7396 0.25233390093676744 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5003 0.17069044164232658 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3551 0.12115166065798555 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.823523551562126E-5 2 0.0 0.0 0.0 0.0 6.823523551562126E-5 3 0.0 0.0 0.0 0.0 6.823523551562126E-5 4 0.0 0.0 0.0 0.0 6.823523551562126E-5 5 0.0 0.0 0.0 0.0 6.823523551562126E-5 6 0.0 0.0 0.0 0.0 6.823523551562126E-5 7 0.0 0.0 0.0 0.0 6.823523551562126E-5 8 0.0 0.0 0.0 0.0 6.823523551562126E-5 9 0.0 0.0 0.0 0.0 6.823523551562126E-5 10-11 0.0 0.0 0.0 3.411761775781063E-5 6.823523551562126E-5 12-13 0.0 0.0 0.0 3.411761775781063E-5 2.8999975094139033E-4 14-15 0.0 0.0 0.0 3.411761775781063E-5 3.7529379533591693E-4 16-17 0.0 0.0 0.0 8.529404439452658E-5 5.288230752460648E-4 18-19 0.0 0.0 0.0 1.5352927991014784E-4 5.799995018827808E-4 20-21 0.0 0.0 0.0 2.217645154257691E-4 6.311759285194966E-4 22-23 0.0 0.0 0.0 3.7529379533591693E-4 7.847052084296445E-4 24-25 0.0 0.0 0.0 4.435290308515382E-4 8.529404439452658E-4 26-27 0.0 0.0 0.0 6.48234737398402E-4 0.0010064697238554136 28-29 0.0 0.0 0.0 0.0018594101678006795 0.001023528532734319 30-31 0.0 0.0 0.0 0.005254113134702837 0.001023528532734319 32-33 0.0 0.0 0.0 0.011480578375503278 0.001023528532734319 34-35 0.0 0.0 0.0 0.022159392733698005 0.0010576461504921296 36-37 0.0 0.0 0.0 0.039815259923365 0.0010576461504921296 38-39 0.0 0.0 0.0 0.06692170723194556 0.0010917637682499402 40-41 0.0 0.0 0.0 0.09583638828169007 0.0010917637682499402 42-43 0.0 0.0 0.0 0.12720753780999694 0.0010917637682499402 44-45 0.0 0.0 0.0 0.1675345619997291 0.0011088225771288453 46-47 0.0 0.0 0.0 0.21782393057474198 0.0011258813860077507 48-49 0.0 0.0 0.0 0.2735380003732467 0.001194116621523372 50-51 0.0 0.0 0.0 0.33326089025829425 0.0012282342392811827 52-53 0.0 0.0 0.0 0.3974020116429783 0.001296469474796804 54-55 0.0 0.0 0.0 0.4701748903203883 0.0013305870925546146 56-57 0.0 0.0 0.0 0.5580277560467507 0.0013305870925546146 58-59 0.0 0.0 0.0 0.65652531851355 0.0013305870925546146 60-61 0.0 0.0 0.0 0.7640981673039269 0.0013305870925546146 62-63 0.0 0.0 3.411761775781063E-5 0.8758504242696357 0.001398822328070236 64-65 0.0 0.0 3.411761775781063E-5 1.0025632566221443 0.0015352927991014785 66-67 0.0 0.0 3.411761775781063E-5 1.1491837189363356 0.001569410416859289 68-69 0.0 0.0 3.411761775781063E-5 1.3179635739842248 0.0016035280346170997 70-71 0.0 0.0 3.411761775781063E-5 1.5078451756153197 0.001671763270132721 72-73 0.0 0.0 3.411761775781063E-5 1.7107085308032617 0.0018082337411639633 74-75 0.0 0.0 3.411761775781063E-5 1.9197301159964886 0.00189352778555849 76-77 0.0 0.0 3.411761775781063E-5 2.1547152083034096 0.0019105865944373953 78-79 0.0 0.0 3.411761775781063E-5 2.4273661506149526 0.0019958806388319216 80-81 0.0 0.0 3.411761775781063E-5 2.7245306012854837 0.0020129394477108272 82-83 0.0 0.0 3.411761775781063E-5 3.031537984679143 0.00214940991874207 84-85 0.0 0.0 3.411761775781063E-5 3.3540859429614844 0.00214940991874207 86-87 0.0 0.0 3.411761775781063E-5 3.692873887296544 0.0022347039631365963 88-89 0.0 0.0 3.411761775781063E-5 4.068542976427796 0.0022858803897733123 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12685 0.0 28.896048 1 GGTATCA 8750 0.0 28.181227 1 ATCAACG 14885 0.0 24.320595 3 TATCAAC 15125 0.0 24.060158 2 TCAACGC 15160 0.0 23.879425 4 CAACGCA 15345 0.0 23.591534 5 AACGCAG 15815 0.0 22.920425 6 GTACATG 9190 0.0 21.134054 1 TACATGG 8860 0.0 21.045406 2 GAGTACT 9965 0.0 21.022005 12-13 ACGCAGA 17745 0.0 20.320581 7 GTACTTT 10275 0.0 20.318499 14-15 CGCAGAG 17850 0.0 20.147886 8 ACATGGG 9000 0.0 19.7164 3 AGAGTAC 14255 0.0 19.671658 10-11 AGTACTT 10550 0.0 19.406582 12-13 GCAGAGT 19295 0.0 18.418335 9 TACTTTT 11990 0.0 16.937346 14-15 ACTTTTT 12490 0.0 16.923395 16-17 GTGTAAG 1300 0.0 16.478107 1 >>END_MODULE