FastQCFastQC Report
Fri 27 May 2016
SRR939068_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939068_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2891991
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92440.3196413820098334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69620.2407338058797555No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45960.15892165639519623No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32900.11376245638385458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88350.029.2387641
GTATCAA128150.029.1253131
TATCAAC150850.024.5879142
ATCAACG151300.024.389553
TCAACGC152300.024.2294084
CAACGCA153150.024.0631715
AACGCAG156550.023.5888216
GTACATG87050.023.1839161
TACATGG85450.022.2299042
GTGTAGC18400.022.1945971
ACGCAGA173550.021.1690737
GAGTACT96000.021.03500412-13
CGCAGAG175350.020.978778
ACATGGG89800.020.6783083
GTACTTT101250.019.75776314-15
AGAGTAC137350.019.6654110-11
GCAGAGT188400.019.2491979
AGTACTT100200.019.0901112-13
CATGGGG55800.017.8274974
TAAGGTG14400.017.763215
Produced by FastQC (version 0.11.5)