FastQCFastQC Report
Fri 27 May 2016
SRR939068_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939068_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2891991
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90920.31438548736839084No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73210.25314739914474144No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48680.16832694154304076No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34960.12088557675317801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91050.031.031871
GTATCAA132050.029.4792791
TCAACGC158950.024.1524164
ATCAACG159100.024.0699943
TATCAAC159950.024.0607532
CAACGCA161750.023.7343225
AACGCAG166300.023.1134826
GAGTACT105750.021.94553412-13
ACGCAGA182200.021.0183097
GTACTTT110750.020.80478114-15
CGCAGAG183900.020.6949928
AGAGTAC147200.020.32766210-11
AGTACTT110050.019.96680612-13
TACATGG83950.019.4451072
GTACATG88050.019.2643261
GCAGAGT198750.019.039
TAAGGTG15000.018.3488715
ACATGGG87600.017.8223273
ACTTTTT129650.017.64288716-17
GAGTACA71350.017.3625131
Produced by FastQC (version 0.11.5)