##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939068_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2891991 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.434004116886946 34.0 31.0 34.0 31.0 34.0 2 32.66822303388911 34.0 31.0 34.0 31.0 34.0 3 32.777414936630166 34.0 31.0 34.0 31.0 34.0 4 36.243245570266296 37.0 35.0 37.0 35.0 37.0 5 36.198221571228956 37.0 35.0 37.0 35.0 37.0 6 36.23291255055773 37.0 37.0 37.0 35.0 37.0 7 36.18639961189368 37.0 37.0 37.0 35.0 37.0 8 36.15727815197212 37.0 37.0 37.0 35.0 37.0 9 37.89508681043613 39.0 38.0 39.0 35.0 39.0 10-11 37.79977427315645 39.0 38.0 39.0 35.0 39.0 12-13 37.70850047596967 39.0 37.5 39.0 35.0 39.0 14-15 39.104951917208595 40.0 38.5 41.0 36.0 41.0 16-17 39.210734058301014 41.0 39.0 41.0 36.0 41.0 18-19 39.233645782438465 41.0 39.0 41.0 36.0 41.0 20-21 39.1909155318948 41.0 39.0 41.0 36.0 41.0 22-23 39.113391258824805 40.0 39.0 41.0 35.5 41.0 24-25 39.030075473955485 40.0 39.0 41.0 35.0 41.0 26-27 38.8699973478479 40.0 38.0 41.0 35.0 41.0 28-29 38.706002024211 40.0 38.0 41.0 34.5 41.0 30-31 38.49540627892687 40.0 38.0 41.0 34.0 41.0 32-33 38.390199692875946 40.0 38.0 41.0 34.0 41.0 34-35 38.27377747717749 40.0 38.0 41.0 34.0 41.0 36-37 38.105255168498104 40.0 38.0 41.0 33.5 41.0 38-39 37.954231531149304 40.0 38.0 41.0 33.0 41.0 40-41 37.74206178373308 40.0 37.0 41.0 33.0 41.0 42-43 37.53204678022857 40.0 37.0 41.0 33.0 41.0 44-45 37.25747607789927 40.0 37.0 41.0 32.0 41.0 46-47 36.94776107532838 40.0 36.0 41.0 31.0 41.0 48-49 36.58221498614622 39.5 36.0 41.0 30.5 41.0 50-51 36.292725323142434 39.0 35.5 41.0 30.0 41.0 52-53 35.986404867788316 39.0 35.0 40.0 29.0 41.0 54-55 35.69031144979358 39.0 35.0 40.0 28.0 41.0 56-57 35.38948824529537 38.0 35.0 40.0 28.0 41.0 58-59 34.996782493444826 38.0 34.0 40.0 27.0 41.0 60-61 34.568242605181 37.5 34.0 40.0 26.0 41.0 62-63 34.11569797416382 37.0 33.5 40.0 26.0 41.0 64-65 33.60951935880852 36.0 33.0 39.0 24.5 40.5 66-67 33.13670460938502 36.0 32.5 39.0 23.0 40.0 68-69 32.58867057331783 35.0 32.0 38.5 22.0 40.0 70-71 32.030162265373576 35.0 31.0 38.0 20.0 40.0 72-73 31.391053775755182 35.0 31.0 37.0 18.0 39.0 74-75 30.803797280143677 34.0 30.0 36.5 14.5 39.0 76-77 28.850010944017463 32.5 28.0 34.5 9.5 36.5 78-79 29.833123616221492 34.0 29.0 35.0 8.0 37.0 80-81 29.8301861935255 34.0 29.5 35.0 7.0 37.0 82-83 29.395057246028774 34.0 29.0 35.0 3.5 36.5 84-85 28.849161529202547 34.0 29.0 35.0 2.0 36.0 86-87 28.065694879410067 33.0 27.5 35.0 2.0 35.5 88-89 27.29172739472564 33.0 26.0 35.0 2.0 35.0 90-91 26.641789168776803 32.0 25.0 34.0 2.0 35.0 92-93 26.077901521823545 32.0 24.0 34.0 2.0 35.0 94-95 25.398760404164467 32.0 21.5 34.0 2.0 35.0 96-97 24.432326552883463 31.0 12.5 34.0 2.0 35.0 98-99 23.00868778637278 31.0 2.0 34.0 2.0 35.0 100-101 17.226219929453446 23.0 2.0 30.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16919167480211428 1101 2 0.17690006054100849 1101 3 0.1683812843100938 1101 4 0.14064032030002238 1101 5 0.16655100042810744 1101 6 0.15913275699683993 1101 7 0.17848585609903722 1101 8 0.2435785672064057 1101 9 0.2191745775370606 1101 10-11 0.22527804199739165 1101 12-13 0.2384973820157228 1101 14-15 0.3420782705434746 1101 16-17 0.28023262637094604 1101 18-19 0.27394318620157065 1101 20-21 0.27169512894135295 1101 22-23 0.2538005586449401 1101 24-25 0.354698815268371 1101 26-27 0.33386974970173355 1101 28-29 0.2834837910903687 1101 30-31 0.3037647789096951 1101 32-33 0.37652331509389825 1101 34-35 0.3631421259153669 1101 36-37 0.3052537193960063 1101 38-39 0.31986092027270985 1101 40-41 0.458612688408472 1101 42-43 0.496859889565215 1101 44-45 0.39203765440074534 1101 46-47 0.4966434723453901 1101 48-49 0.5347758144357897 1101 50-51 0.4478334939733841 1101 52-53 0.40219614153495087 1101 54-55 0.32835307729855856 1101 56-57 0.42897821177008666 1101 58-59 0.3928642400362179 1101 60-61 0.49217772719295994 1101 62-63 0.29039733483978125 1101 64-65 0.2077830878326168 1101 66-67 0.2876666587658434 1101 68-69 0.23067402706753626 1101 70-71 0.30007251004646207 1101 72-73 0.28717547005311417 1101 74-75 0.05258075284018204 1101 76-77 0.1258188771749822 1101 78-79 0.2634496111726854 1101 80-81 0.2836391005583181 1101 82-83 0.0023904687657534396 1101 84-85 0.10100955626141328 1101 86-87 0.27268957958341744 1101 88-89 0.41936728898473064 1101 90-91 0.5447882155998123 1101 92-93 0.3260099723039964 1101 94-95 0.16875026304098384 1101 96-97 0.14520801300361796 1101 98-99 -0.4199238208339686 1101 100-101 -1.1181887285592325 1102 1 0.16301756855745708 1102 2 0.13327104797028966 1102 3 0.12370459608926154 1102 4 0.08931950008965828 1102 5 0.17436618210525978 1102 6 0.16371720652551147 1102 7 0.20536224936482483 1102 8 0.17641769410115415 1102 9 0.20366851468362768 1102 10-11 0.2267063396780955 1102 12-13 0.2745652954858997 1102 14-15 0.3149816878190208 1102 16-17 0.3180526364663123 1102 18-19 0.3100138018565417 1102 20-21 0.26739880951777906 1102 22-23 0.23077369784714108 1102 24-25 0.2903752362045324 1102 26-27 0.23400217219805342 1102 28-29 0.27799511332188587 1102 30-31 0.15854782358020714 1102 32-33 0.28120143006830745 1102 34-35 0.2537109759627896 1102 36-37 0.2084811471585013 1102 38-39 0.26719943037448246 1102 40-41 0.2363670215162159 1102 42-43 0.36621543084294217 1102 44-45 0.24206523984729955 1102 46-47 0.28201758056649595 1102 48-49 0.2645813451441086 1102 50-51 0.15076286303176545 1102 52-53 0.0922662763554456 1102 54-55 0.10268791134812716 1102 56-57 0.10021743835229557 1102 58-59 -0.0542079118494172 1102 60-61 -0.17002168756012992 1102 62-63 -0.11796555653501883 1102 64-65 -0.10154897686356179 1102 66-67 -0.07662799920009178 1102 68-69 0.05643699666671864 1102 70-71 -0.017456053204966793 1102 72-73 0.06356203441969654 1102 74-75 -0.06291440862771225 1102 76-77 0.025520880235088583 1102 78-79 0.035709630526501 1102 80-81 0.21470984051379816 1102 82-83 -0.03715500213318279 1102 84-85 -0.04649136286744593 1102 86-87 0.16023923994967149 1102 88-89 0.1276827299944152 1102 90-91 0.39831729211194045 1102 92-93 0.1368637530697221 1102 94-95 0.2727981976376128 1102 96-97 0.33025015396161805 1102 98-99 0.0998290728585296 1102 100-101 -0.28995905026282287 1103 1 0.035391915641184823 1103 2 0.0845097388769318 1103 3 0.09270363228196743 1103 4 0.12578760360327834 1103 5 0.1224351984081622 1103 6 0.13105003529389592 1103 7 0.0906600068583927 1103 8 0.1118895325184397 1103 9 0.17225047259617554 1103 10-11 0.1328702693406214 1103 12-13 0.1521022835459931 1103 14-15 -0.010194002458312923 1103 16-17 0.14527664284837272 1103 18-19 0.2391428317081008 1103 20-21 0.19318557287000004 1103 22-23 0.17794140091749 1103 24-25 0.14749095564877024 1103 26-27 0.1624662532300576 1103 28-29 0.16771505871817283 1103 30-31 0.1549223409245215 1103 32-33 0.28273091763808367 1103 34-35 0.28967183227127435 1103 36-37 0.18071520974461208 1103 38-39 0.29341474805185896 1103 40-41 0.2964772065888681 1103 42-43 0.22044093166778111 1103 44-45 0.2871933203306227 1103 46-47 0.3673549101103859 1103 48-49 0.18399356407877576 1103 50-51 0.23522497493689087 1103 52-53 0.22264602966409797 1103 54-55 0.09822368472225662 1103 56-57 0.05405147518874287 1103 58-59 0.02080669265869517 1103 60-61 0.041214247892447986 1103 62-63 0.09091019414572798 1103 64-65 0.04093860554437612 1103 66-67 -0.18749035404241 1103 68-69 -0.08380170206108062 1103 70-71 -0.22114712830131822 1103 72-73 -0.2946705313171343 1103 74-75 -0.34263172491172256 1103 76-77 -0.20896434920759788 1103 78-79 -0.22101054890288552 1103 80-81 -0.0025816263623994473 1103 82-83 -0.19165588291587454 1103 84-85 -0.40910302493567485 1103 86-87 -0.29628519996518676 1103 88-89 -0.18882110116964768 1103 90-91 -0.15556055824885462 1103 92-93 -0.1890525406881558 1103 94-95 -0.2120240936247093 1103 96-97 -0.43290247653439806 1103 98-99 -0.7870614182981477 1103 100-101 -1.0764045175409045 1104 1 0.08001674315281804 1104 2 0.04446892936285707 1104 3 0.10052878608164662 1104 4 0.017988249597202355 1104 5 0.04443695679044879 1104 6 -0.01755324968392813 1104 7 0.07614894527034721 1104 8 0.056924805949236656 1104 9 0.12866204453822405 1104 10-11 0.17161153913711047 1104 12-13 0.02961776342215927 1104 14-15 -0.005771159708942264 1104 16-17 -0.025156558606283852 1104 18-19 0.13442652365268515 1104 20-21 0.15987871517908303 1104 22-23 0.10651960896608159 1104 24-25 0.05173052583243276 1104 26-27 0.05443505369833446 1104 28-29 0.08634800464322012 1104 30-31 0.10658871962083083 1104 32-33 0.045303174691007086 1104 34-35 0.04349816498635306 1104 36-37 0.06866344442752137 1104 38-39 0.045747895673692085 1104 40-41 -0.007737768575154291 1104 42-43 0.04694437203205837 1104 44-45 0.003964479099856533 1104 46-47 -0.05096332534829173 1104 48-49 -0.062300286754933154 1104 50-51 -0.1809049796225608 1104 52-53 -0.35860422010611614 1104 54-55 -0.17639572424502603 1104 56-57 -0.33566865875860685 1104 58-59 -0.39878919089314024 1104 60-61 -0.4194055113408197 1104 62-63 -0.3184097021379273 1104 64-65 -0.36131764223708274 1104 66-67 -0.4087729505242379 1104 68-69 -0.5393897669237617 1104 70-71 -0.3814928455355826 1104 72-73 -0.8417858616837854 1104 74-75 -0.8485899198429756 1104 76-77 -0.6800427218106861 1104 78-79 -0.4226068450163467 1104 80-81 -0.3602487490800854 1104 82-83 -0.43235576255602126 1104 84-85 -0.7785891271334151 1104 86-87 -0.7001427686490587 1104 88-89 -0.30943675241756097 1104 90-91 -0.22582650181606567 1104 92-93 -0.5014405848639818 1104 94-95 -0.30546606818458244 1104 96-97 -0.7642197234907293 1104 98-99 -1.0664830268888288 1104 100-101 -1.3025627754730191 1105 1 -0.004686009166221083 1105 2 0.056674253989008605 1105 3 0.06953549461326958 1105 4 0.09268413959846811 1105 5 -0.026355512049448748 1105 6 0.0613980986638083 1105 7 0.1258741268612127 1105 8 0.040749079635354235 1105 9 0.11578082550963131 1105 10-11 0.11811321563278199 1105 12-13 0.10356405212041153 1105 14-15 0.160123248394207 1105 16-17 0.1832338072999704 1105 18-19 0.16484736527362287 1105 20-21 0.11532406204332801 1105 22-23 0.1468662423945517 1105 24-25 0.11191394485269512 1105 26-27 0.19250910725499892 1105 28-29 0.14123897082527037 1105 30-31 0.17781571223484605 1105 32-33 0.1651605075859237 1105 34-35 0.1186246137411544 1105 36-37 0.07577300955822608 1105 38-39 -0.032910395170198115 1105 40-41 -0.03879931504080503 1105 42-43 -0.1276159425866652 1105 44-45 -0.0575691002346872 1105 46-47 0.06984299274208894 1105 48-49 0.06778255102162944 1105 50-51 0.0532080325271167 1105 52-53 0.033225840810757745 1105 54-55 -0.07792111014772729 1105 56-57 -0.10637534154591322 1105 58-59 -0.21746193140491243 1105 60-61 -0.15577886844161526 1105 62-63 -0.15138682085376587 1105 64-65 -0.25692537651952563 1105 66-67 -0.22191229239312804 1105 68-69 -0.12593030628551105 1105 70-71 -0.24174399560396154 1105 72-73 -0.344229447229246 1105 74-75 -0.33497269386767314 1105 76-77 -0.592829191007997 1105 78-79 -0.7510698585962565 1105 80-81 -0.4308079189407188 1105 82-83 -0.47599633683827136 1105 84-85 -0.577730105422944 1105 86-87 -0.37579509242018005 1105 88-89 -0.4900263056645322 1105 90-91 -0.37175830572767055 1105 92-93 -0.5469079715783884 1105 94-95 -0.538985955736603 1105 96-97 -0.8043004173240185 1105 98-99 -0.8173423742974215 1105 100-101 -1.5110742869190439 1106 1 0.023049021006862347 1106 2 0.02413708278533022 1106 3 0.0489356777898422 1106 4 0.059647583054569964 1106 5 0.05029430533662804 1106 6 0.04371801871823777 1106 7 0.07516503810291653 1106 8 0.014787766286211479 1106 9 0.09638000008975212 1106 10-11 0.053885454199281924 1106 12-13 0.13484219314990042 1106 14-15 0.0047660334905330615 1106 16-17 0.004596576235925909 1106 18-19 0.006734816800026522 1106 20-21 0.06120724127944044 1106 22-23 0.10017282107936865 1106 24-25 0.12244152028266342 1106 26-27 0.08213321377517246 1106 28-29 0.14958415409653725 1106 30-31 0.056404006554643615 1106 32-33 0.06321254795722098 1106 34-35 -0.03385629804672874 1106 36-37 0.035598632266633956 1106 38-39 0.13295483967814192 1106 40-41 0.10085909934829829 1106 42-43 0.007787129586937169 1106 44-45 0.11747794957677371 1106 46-47 0.018076535463208643 1106 48-49 -0.17172308356553145 1106 50-51 -0.08937814678117917 1106 52-53 0.04701863335876055 1106 54-55 -0.006990208665932585 1106 56-57 -0.10760459248986365 1106 58-59 -0.29870680752469525 1106 60-61 -0.2265010408488095 1106 62-63 -0.2207963055797606 1106 64-65 -0.3685150824022898 1106 66-67 -0.21671787518913277 1106 68-69 -0.33677109883571177 1106 70-71 -0.29871138858193547 1106 72-73 -0.4247340967688764 1106 74-75 -0.4022342315353775 1106 76-77 -0.5056158435453106 1106 78-79 -0.30260453191261405 1106 80-81 -0.2186725532641205 1106 82-83 -0.311718184006871 1106 84-85 -0.4412614994559547 1106 86-87 -0.41777859225646097 1106 88-89 -0.4776806876439501 1106 90-91 -0.16551483623052476 1106 92-93 -0.18070989550878735 1106 94-95 -0.3924207497892098 1106 96-97 -0.685370542882584 1106 98-99 -0.4872713762211305 1106 100-101 -1.3643582635798097 1107 1 0.04876406083793938 1107 2 0.05139177773366299 1107 3 0.03935302430422638 1107 4 -0.01866207558189359 1107 5 -0.002164343925933565 1107 6 0.051034779763760696 1107 7 0.07397090558859531 1107 8 0.1124346933745457 1107 9 0.1536207781554637 1107 10-11 0.11836181149524094 1107 12-13 0.10715399530621994 1107 14-15 0.16613468691216582 1107 16-17 0.1482324018110006 1107 18-19 0.135658737841716 1107 20-21 0.1010803097121098 1107 22-23 0.09333995164212894 1107 24-25 0.09775904915619549 1107 26-27 0.10866527059445019 1107 28-29 -0.008737581834616037 1107 30-31 0.02765644600820849 1107 32-33 -0.010578522842919824 1107 34-35 -0.007996090733165317 1107 36-37 0.11601661863768697 1107 38-39 0.07991192129186686 1107 40-41 0.1191641990059793 1107 42-43 0.1737943439709113 1107 44-45 0.10832839907607905 1107 46-47 0.18661838208956283 1107 48-49 0.10731321230419155 1107 50-51 -0.010740161689838601 1107 52-53 0.11611475582733277 1107 54-55 -0.03792967822475646 1107 56-57 -0.045064601447862174 1107 58-59 -0.13183778618547137 1107 60-61 -0.23155350909592443 1107 62-63 -0.05401846381631259 1107 64-65 0.020622950678308882 1107 66-67 -0.2606720294585756 1107 68-69 -0.44552153873716804 1107 70-71 -0.4329421208978985 1107 72-73 -0.5275394683656387 1107 74-75 -0.5509297237451243 1107 76-77 -0.47591649619911536 1107 78-79 -0.3129150223627555 1107 80-81 -0.15111608591281112 1107 82-83 -0.2965443829383112 1107 84-85 -0.28247146842290505 1107 86-87 -0.4372990196807187 1107 88-89 -0.627889840793646 1107 90-91 -0.2907211900072788 1107 92-93 -0.20547354845052368 1107 94-95 -0.3669748496708358 1107 96-97 -0.6720211331503627 1107 98-99 -0.7357961066815086 1107 100-101 -1.5350592265641012 1108 1 -0.09973933735589924 1108 2 -0.030664517459214835 1108 3 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1.4981752377345288 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 30.0 5 50.0 6 120.0 7 288.0 8 769.0 9 1439.0 10 2526.0 11 3897.0 12 5882.0 13 8225.0 14 10736.0 15 14277.0 16 18498.0 17 25190.0 18 25683.0 19 20620.0 20 19649.0 21 20959.0 22 22911.0 23 25044.0 24 27947.0 25 31953.0 26 37161.0 27 44320.0 28 52911.0 29 63990.0 30 77539.0 31 95007.0 32 119324.0 33 153757.0 34 211586.0 35 309418.0 36 472636.0 37 630299.0 38 326524.0 39 10819.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.16763053949101 19.356587112437015 15.230093387332433 23.245688960739542 2 15.630373676819879 20.32236614844237 40.13408063856353 23.913179536174216 3 19.71389260893274 23.77389832817599 29.49117061567619 27.021038447215084 4 13.558202636176944 16.920073402718057 36.27462879379638 33.24709516730861 5 14.230058115671868 36.749872319796296 34.03260936842473 14.987460196107111 6 32.51579966880948 35.86746985035569 16.998151100746856 14.618579380087974 7 28.740511294813846 32.148267404704924 20.498611510201794 18.61260979027943 8 25.872556311551453 35.6688177798617 19.36302706336223 19.09559884522462 9 25.774423226075044 17.542447400424138 20.3436663530419 36.339463020458915 10-11 25.015274943801693 26.504110835752947 26.972940095595042 21.507674124850322 12-13 25.99670953332842 23.670958865362998 27.567686068179327 22.76464553312925 14-15 22.594892643083053 25.26229998248596 25.962999904390283 26.179807470040707 16-17 22.19948456442305 28.262802986109936 26.819265243230888 22.718447206236128 18-19 22.170677571264918 26.978887555320885 28.99396989824657 21.85646497516763 20-21 22.71397110156982 26.08746016152886 29.274416829098016 21.92415190780331 22-23 22.355705809596227 25.92724182060041 29.20451688819225 22.512535481611113 24-25 22.471170370575276 26.142742540603532 29.187341588318077 22.198745500503115 26-27 22.165748061038432 26.362183394003146 29.133768551493954 22.338299993464467 28-29 22.048623957897505 26.875525157417556 28.930528805424636 22.145322079260303 30-31 22.3240494178578 26.38758211903149 28.836725287181046 22.45164317592966 32-33 22.06879656070793 26.389471196395704 28.89169241738519 22.650039825511183 34-35 22.617059530845438 26.509764226160566 28.7349993619509 22.138176881043094 36-37 22.699981703269927 26.16693535305253 28.807769158215525 22.32531378546202 38-39 22.24931205673636 26.53250137433019 28.77514540058937 22.44304116834408 40-41 22.649110824853675 26.455852475598473 28.646050367860017 22.248986331687835 42-43 22.44350547952714 26.5446308138223 28.332685218149695 22.67917848850087 44-45 22.4985822371606 26.62268143906386 28.5072513382298 22.371484985545735 46-47 22.861908507273345 26.842473724642097 27.95784185456557 22.337775913518986 48-49 22.63361294208855 26.949135135471586 27.85575424859383 22.561497673846034 50-51 22.67012152099114 27.11465318012517 27.858056594303637 22.357168704580044 52-53 22.94756449795314 26.997283186565934 27.642582566819883 22.412569748661042 54-55 22.589904325428396 26.978818398812443 27.818585880799766 22.612691394959388 56-57 22.670258657578113 27.118580478940192 27.66081965373664 22.550341209745056 58-59 22.751111710478085 26.86037746287959 27.661068348582614 22.72744247805971 60-61 22.459649051828574 27.035848471692653 28.012660151591934 22.491842324886836 62-63 22.419334649170732 27.02448315114933 28.031641647775135 22.524540551904806 64-65 22.30904701852033 27.090483325339747 27.862846713738325 22.737622942401593 66-67 22.372387169680053 27.07407265249413 27.634182834929327 22.919357342896493 68-69 22.510467355130697 26.87617053178452 27.879075940367542 22.734286172717237 70-71 22.503209298103812 26.903591388275366 27.642114635696043 22.951084677924783 72-73 22.51212054256047 26.877123753151377 27.688588934059617 22.92216677022854 74-75 22.38729304482621 27.310371989401073 27.40366411928668 22.898670846486034 76-77 22.448068772319406 26.93057008232438 27.558957146051196 23.062403999305022 78-79 22.422866521922874 27.0582415081759 27.61942919116625 22.899462778734975 80-81 22.59709610716533 27.050014177138156 27.409836859176067 22.943052856520445 82-83 22.521733297233638 27.04913327876885 27.593723493606998 22.835409930390515 84-85 22.44865215693963 27.15959351187469 27.442512787902867 22.94924154328281 86-87 22.470854563579973 27.046999612317236 27.454637655073107 23.027508169029687 88-89 22.379815116337408 27.06865701283846 27.163097014012017 23.388430856812118 90-91 22.317032583270702 27.025501573893663 27.334828840475012 23.322637002360626 92-93 22.418569075768215 26.88080633722581 27.384058249143926 23.316566337862046 94-95 22.19066763877682 26.6897660970878 27.544315930498385 23.575250333636987 96-97 22.190671475350882 26.41266048637789 27.70747824162601 23.689189796645216 98-99 21.99812412743831 26.654731391473856 27.390384640320885 23.956759840766946 100-101 22.524329788644767 25.412092305633564 28.19459819822374 23.868979707497925 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 729.0 1 513.5 2 372.0 3 477.5 4 442.0 5 401.5 6 677.5 7 1537.5 8 2581.0 9 2982.0 10 2622.5 11 2195.0 12 2201.0 13 2396.5 14 2608.0 15 2794.5 16 3013.5 17 3310.5 18 3694.5 19 4252.5 20 4852.5 21 5652.5 22 6893.0 23 8689.0 24 10897.5 25 13569.5 26 17401.5 27 21818.5 28 25376.5 29 29352.5 30 33874.0 31 39227.5 32 46459.0 33 50800.5 34 53450.5 35 57602.0 36 62978.0 37 68253.0 38 72205.5 39 75553.0 40 79871.5 41 85220.5 42 88429.5 43 91847.5 44 96409.5 45 101625.0 46 105361.5 47 109159.0 48 115453.0 49 115093.5 50 109080.5 51 105413.0 52 104075.5 53 103400.5 54 101473.0 55 94732.5 56 86357.0 57 77851.0 58 70552.5 59 63306.5 60 53047.5 61 44306.0 62 36783.5 63 27888.5 64 19691.5 65 14449.5 66 10789.5 67 8512.5 68 6793.5 69 5404.5 70 4209.0 71 2921.5 72 2107.0 73 1721.0 74 1358.0 75 999.0 76 695.0 77 458.5 78 306.0 79 187.5 80 118.5 81 76.5 82 44.5 83 26.0 84 12.5 85 9.0 86 7.5 87 4.5 88 3.5 89 2.5 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.41296809014965813 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.817454826104231E-4 16-17 0.002351321286961128 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.457825422001659E-5 26-27 0.0037344514557617918 28-29 3.457825422001659E-5 30-31 0.0 32-33 1.7289127110008296E-5 34-35 0.012586484536086039 36-37 0.026417786224092677 38-39 0.020141833083159665 40-41 0.009197815622524414 42-43 0.00942257427495452 44-45 0.0038381862184218413 46-47 0.005722701073412746 48-49 0.0032157776424615427 50-51 7.434324657303568E-4 52-53 0.0 54-55 0.0 56-57 5.186738133002488E-5 58-59 0.0017634909652208461 60-61 0.0028527059731513686 62-63 0.0014868649314607135 64-65 0.00304288637136146 66-67 0.0020746952532009954 68-69 0.004944690353462373 70-71 1.2102388977005808E-4 72-73 0.0 74-75 0.0 76-77 0.09298092559762461 78-79 0.015387323127907384 80-81 3.803607964201825E-4 82-83 0.0 84-85 0.0 86-87 0.10480668854087027 88-89 0.40994249290540674 90-91 0.4160282656481296 92-93 0.0 94-95 1.7289127110008296E-5 96-97 3.457825422001659E-5 98-99 1.2102388977005808E-4 100-101 3.9764992353019077E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2891991.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.6746834777449 #Duplication Level Percentage of deduplicated Percentage of total 1 74.07024900083965 40.49767419238658 2 14.442340288141217 15.792607678640083 3 4.899166170706734 8.035810790647988 4 2.2394501475855764 4.8976491193372205 5 1.2100876060503318 3.308057842057198 6 0.7335735481140204 2.40647409304682 7 0.48860488130799945 1.8700022061837192 8 0.3290911783208455 1.4394364808008269 9 0.24287283602093196 1.1951095891308028 >10 1.2406453936573076 11.999811542974276 >50 0.06921945199002041 2.583189240075347 >100 0.030151201909739 2.9413587482802517 >500 0.003158538441512764 1.2492200373150995 >1k 0.0012634153765458383 1.2167193860200387 >5k 1.2634153765458383E-4 0.5668790531039769 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9092 0.31438548736839084 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7321 0.25314739914474144 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4868 0.16832694154304076 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3496 0.12088557675317801 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.457825422001659E-5 2 0.0 0.0 0.0 0.0 3.457825422001659E-5 3 0.0 0.0 0.0 0.0 3.457825422001659E-5 4 0.0 0.0 0.0 0.0 3.457825422001659E-5 5 0.0 0.0 0.0 0.0 1.0373476266004977E-4 6 0.0 0.0 0.0 0.0 1.3831301688006637E-4 7 0.0 0.0 0.0 0.0 1.3831301688006637E-4 8 0.0 0.0 0.0 3.457825422001659E-5 1.3831301688006637E-4 9 0.0 0.0 0.0 3.457825422001659E-5 1.3831301688006637E-4 10-11 0.0 0.0 0.0 3.457825422001659E-5 1.3831301688006637E-4 12-13 0.0 0.0 0.0 6.915650844003319E-5 5.013846861902406E-4 14-15 0.0 0.0 0.0 6.915650844003319E-5 6.742759572903235E-4 16-17 0.0 0.0 0.0 8.644563555004149E-5 7.60721592840365E-4 18-19 0.0 0.0 0.0 1.3831301688006637E-4 8.298781012803982E-4 20-21 0.0 0.0 0.0 1.5560214399007466E-4 9.163237368304397E-4 22-23 0.0 0.0 0.0 1.7289127110008294E-4 9.509019910504562E-4 24-25 0.0 0.0 0.0 2.5933690665012443E-4 9.681911181604646E-4 26-27 0.0 0.0 0.0 5.359629404102571E-4 0.0010719258808205142 28-29 0.0 0.0 0.0 0.0015214431856807298 0.0010719258808205142 30-31 0.0 0.0 0.0 0.005013846861902406 0.0010719258808205142 32-33 0.0 0.0 0.0 0.01227528024810589 0.0011237932621505392 34-35 0.0 0.0 0.0 0.022908093420760993 0.0012448171519205973 36-37 0.0 0.0 0.0 0.041321013792919824 0.0012448171519205973 38-39 0.0 0.0 0.0 0.0702284343208537 0.0012793954061406138 40-41 0.0 0.0 0.0 0.09894567445057748 0.0013139736603606305 42-43 0.0 0.0 0.0 0.1315356790529431 0.001348551914580647 44-45 0.0 0.0 0.0 0.17273566895609288 0.0014004192959106718 46-47 0.0 0.0 0.0 0.22341010051552718 0.0014177084230206803 48-49 0.0 0.0 0.0 0.2801011483092444 0.0014177084230206803 50-51 0.0 0.0 0.0 0.3428433905914645 0.0014522866772406968 52-53 0.0 0.0 0.0 0.4075393042371155 0.0016251779483407798 54-55 0.0 0.0 0.0 0.48106996183598083 0.0016943344567808129 56-57 0.0 0.0 0.0 0.5718551682906343 0.0017116235838908213 58-59 0.0 0.0 0.0 0.6716825882238223 0.0017289127110008296 60-61 0.0 0.0 0.0 0.7840273361846561 0.0017289127110008296 62-63 0.0 0.0 0.0 0.9000719573470318 0.0017289127110008296 64-65 0.0 0.0 0.0 1.0257466223096823 0.0019190931092109209 66-67 0.0 0.0 0.0 1.1760064260227643 0.0019536713634309374 68-69 3.457825422001659E-5 0.0 0.0 1.3495028165716976 0.001970960490540946 70-71 3.457825422001659E-5 0.0 0.0 1.5436942922713106 0.002022827871870971 72-73 3.457825422001659E-5 0.0 0.0 1.7412398586302655 0.0022475865243010784 74-75 3.457825422001659E-5 0.0 0.0 1.946686556078494 0.00243776692251117 76-77 3.457825422001659E-5 0.0 0.0 2.1810925414359863 0.0024896343038411945 78-79 3.457825422001659E-5 0.0 0.0 2.457683996941899 0.002524212558061211 80-81 3.457825422001659E-5 0.0 0.0 2.756249933004632 0.002576079939391236 82-83 3.457825422001659E-5 0.0 0.0 3.0666416320106116 0.0025933690665012445 84-85 3.457825422001659E-5 0.0 0.0 3.388962828722496 0.002627947320721261 86-87 3.457825422001659E-5 0.0 0.0 3.726446589909858 0.0026625255749412775 88-89 3.457825422001659E-5 0.0 0.0 4.102467815425428 0.002697103829161294 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9105 0.0 31.03187 1 GTATCAA 13205 0.0 29.479279 1 TCAACGC 15895 0.0 24.152416 4 ATCAACG 15910 0.0 24.069994 3 TATCAAC 15995 0.0 24.060753 2 CAACGCA 16175 0.0 23.734322 5 AACGCAG 16630 0.0 23.113482 6 GAGTACT 10575 0.0 21.945534 12-13 ACGCAGA 18220 0.0 21.018309 7 GTACTTT 11075 0.0 20.804781 14-15 CGCAGAG 18390 0.0 20.694992 8 AGAGTAC 14720 0.0 20.327662 10-11 AGTACTT 11005 0.0 19.966806 12-13 TACATGG 8395 0.0 19.445107 2 GTACATG 8805 0.0 19.264326 1 GCAGAGT 19875 0.0 19.03 9 TAAGGTG 1500 0.0 18.348871 5 ACATGGG 8760 0.0 17.822327 3 ACTTTTT 12965 0.0 17.642887 16-17 GAGTACA 7135 0.0 17.362513 1 >>END_MODULE