FastQCFastQC Report
Fri 27 May 2016
SRR939067_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939067_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381160
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92152.4176198971560496No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA83302.1854339385035155No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68981.809738692412635No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52521.3778990450204638No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37600.9864623780039877No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19430.5097596809738693No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16080.4218700808059608No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13100.34368769020883616No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11900.31220484835764506No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9640.2529121628712352No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA6040.15846363731766186No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA5990.1571518522405289No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5940.15584006716339596No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA5230.1372127190681079No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC5100.13380207786756218No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG4880.12803022352817714No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG4800.1259313674047644No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT4800.1259313674047644No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG4690.12304544023507188No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA4460.11701122888026025No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC4440.11648651484940707No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG4410.11569944380312729No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT4290.11255115961800818No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT4120.1080910903557561No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT4070.10677930527862316No Hit
TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA4020.1054675202014902No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG3970.10415573512435722No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT3860.10126980795466471No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGGT851.8189894E-1255.8703581
GTAAGGT802.2963468E-741.4717254
GTGGCTC853.706391E-739.016849
TCTTCGG757.0335536E-637.9020738
CTTCGGC801.0949094E-535.5331959
TATGGTG1850.033.3054772
GGTATCA37050.032.8134651
TACTCCC1456.7666406E-1032.6870735
ATGGTGG1753.6379788E-1232.500293
CCGTTCA300.003933935431.69322836-37
GGTGGTA1509.731593E-1031.5975075
GGGACCA600.003997843731.585068
GGACCAC600.003997843731.585069
TGGGAAC1051.9378385E-631.5809156
GTGGTAC1551.3897079E-930.5621786
TAAGGTG1252.1890628E-730.3336035
ATAGGGA804.5458935E-429.6226623
TGGGAGT804.5598985E-429.607116
GGTACTC1452.442539E-829.4183673
TGGTACT1452.442539E-829.4183672
Produced by FastQC (version 0.11.5)