FastQCFastQC Report
Fri 27 May 2016
SRR939067_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939067_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381160
Sequences flagged as poor quality0
Sequence length101
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91702.4058138314618533No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA75681.985517892748452No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72291.8965788645188373No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58511.5350508972609926No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38411.0077132962535418No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22270.5842690733550214No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16180.4244936509602267No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15290.40114387658726No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12040.3158778465736174No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10750.28203379158358693No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6440.16895791793472556No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT6220.16318606359534055RNA PCR Primer, Index 26 (100% over 22bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA6030.1582012803022353No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5780.15164235491657047No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA5730.1503305698394375No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA5690.14928114177773114No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG5050.13249029279042923No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC4980.13065379368244306No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT4850.12724315248189738No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG4770.12514429635848462No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT4560.11963479903452619No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG4460.11701122888026025No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA4400.1154370867877007No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT4120.1080910903557561No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC4020.1054675202014902No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC3860.10126980795466471No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAT502.8390841E-547.455537
AGAACCG704.3303826E-640.6761675
GGATTTA608.16649E-539.676391
GAACCGA651.333849E-436.5042536
GGTACTC1700.036.2895243
TGGTACT1750.035.252682
GCTTACG550.002547949834.6266751
ACCGATT550.002589337834.5131158
TATAGGT550.002589337834.5131153
CTTACGC550.002589337834.5131152
GGTATCA39900.032.6957361
CGGAGGT753.0891763E-431.641179
GCAGATC753.091555E-431.637023
CTTAAGT600.00396581131.6370188
ACGCCAA600.00396581131.6370185
GGGAGAT600.00396581131.6370187
GGGACTA600.00396581131.6370187
CCCAACG600.00396581131.6370187
ACTACCT1657.6397555E-1131.6370184
GGACAAT2300.030.9492596
Produced by FastQC (version 0.11.5)