##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939067_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 381160 Sequences flagged as poor quality 0 Sequence length 101 %GC 34 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.92376954559765 31.0 31.0 34.0 30.0 34.0 2 32.148126770909855 33.0 31.0 34.0 30.0 34.0 3 32.1616224157834 34.0 31.0 34.0 30.0 34.0 4 35.85965998530801 37.0 35.0 37.0 35.0 37.0 5 35.82328943225942 37.0 35.0 37.0 35.0 37.0 6 35.9018128869766 37.0 35.0 37.0 35.0 37.0 7 35.84037674467415 37.0 35.0 37.0 35.0 37.0 8 35.850131178507716 37.0 35.0 37.0 35.0 37.0 9 37.44936771959282 39.0 37.0 39.0 34.0 39.0 10-11 37.37412897470878 39.0 37.0 39.0 34.0 39.0 12-13 37.28103289956974 39.0 37.0 39.0 34.0 39.0 14-15 38.303452618323014 40.0 38.0 41.0 33.0 41.0 16-17 38.223189736593554 40.0 37.5 41.0 33.0 41.0 18-19 38.321421450309586 40.0 37.5 41.0 33.5 41.0 20-21 38.41693908070101 40.0 38.0 41.0 34.0 41.0 22-23 38.30292790429216 40.0 37.5 41.0 34.0 41.0 24-25 38.173689526707946 40.0 37.0 41.0 33.0 41.0 26-27 37.91813018155106 40.0 37.0 41.0 33.0 41.0 28-29 37.64886137055305 39.5 36.0 41.0 33.0 41.0 30-31 37.21880312729562 39.0 35.5 40.5 33.0 41.0 32-33 36.725498478329314 38.0 35.0 40.0 31.5 41.0 34-35 36.448353709728195 38.0 35.0 40.0 31.0 41.0 36-37 36.205481949837335 38.0 35.0 40.0 30.5 41.0 38-39 35.94610531010599 38.0 35.0 40.0 30.0 41.0 40-41 35.60365069786966 38.0 35.0 40.0 30.0 41.0 42-43 35.26529410221429 38.0 35.0 40.0 28.5 41.0 44-45 34.78060919298982 38.0 34.5 40.0 25.5 41.0 46-47 34.158279987406864 38.0 33.5 40.0 23.0 41.0 48-49 33.56009943330885 37.5 33.0 40.0 19.5 41.0 50-51 33.176836499107985 37.0 33.0 40.0 15.0 41.0 52-53 32.867159460593975 37.0 33.0 40.0 11.0 41.0 54-55 32.58689920243467 37.0 33.0 40.0 8.5 41.0 56-57 32.23390964424389 36.0 32.5 40.0 4.5 41.0 58-59 31.84321020044076 36.0 31.5 39.0 2.0 41.0 60-61 31.404181970825903 35.0 31.0 39.0 2.0 40.0 62-63 30.90136294469514 35.0 30.5 39.0 2.0 40.0 64-65 30.37691258264246 35.0 29.0 38.0 2.0 40.0 66-67 29.950001311785076 35.0 29.0 38.0 2.0 40.0 68-69 29.400663763249028 35.0 28.0 37.5 2.0 40.0 70-71 28.900247927379578 34.0 27.5 37.0 2.0 39.0 72-73 28.32943252177563 34.0 26.0 36.0 2.0 39.0 74-75 27.750902508133066 33.5 26.0 36.0 2.0 39.0 76-77 25.91115542029594 31.5 23.0 34.5 2.0 36.0 78-79 26.865503987826635 33.0 25.0 35.0 2.0 37.0 80-81 26.82118795256585 33.0 25.0 35.0 2.0 37.0 82-83 26.418401721062022 33.0 23.5 35.0 2.0 36.5 84-85 25.860293577500265 33.0 21.0 35.0 2.0 36.0 86-87 25.099111921502782 32.0 17.5 35.0 2.0 35.5 88-89 24.34734232343373 31.5 8.5 35.0 2.0 35.0 90-91 23.665231136530593 31.0 2.0 34.0 2.0 35.0 92-93 23.129672578444747 31.0 2.0 34.0 2.0 35.0 94-95 22.581959019834187 31.0 2.0 34.0 2.0 35.0 96-97 21.716519309476332 30.0 2.0 34.0 2.0 35.0 98-99 20.63488823591143 29.0 2.0 34.0 2.0 35.0 100-101 17.34343845104418 22.5 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1577785372288929 1101 2 0.19423474950944808 1101 3 0.2583962871320722 1101 4 0.1916374897880786 1101 5 0.1848849630057714 1101 6 0.17345525495169767 1101 7 0.27815893554423354 1101 8 0.141006278499475 1101 9 0.5570508204618321 1101 10-11 0.2863147109272077 1101 12-13 0.36417647879036963 1101 14-15 0.706057704472947 1101 16-17 0.4602312001718545 1101 18-19 0.4349362447623051 1101 20-21 0.4671814071729088 1101 22-23 0.6138777482924098 1101 24-25 0.4111207811005855 1101 26-27 0.655726795291983 1101 28-29 0.4442635352146027 1101 30-31 0.7773170065768582 1101 32-33 0.6894032383836546 1101 34-35 0.737737915160352 1101 36-37 0.6821664942351973 1101 38-39 0.8168504437784563 1101 40-41 0.48953780406025516 1101 42-43 0.8085312755095657 1101 44-45 0.9721468549009202 1101 46-47 1.259786348003196 1101 48-49 1.1976920022019257 1101 50-51 1.3175655917339029 1101 52-53 1.0196096573346267 1101 54-55 1.1756059963549035 1101 56-57 1.3252678555959676 1101 58-59 0.8900743124659307 1101 60-61 0.8760818341052037 1101 62-63 1.0203204080736832 1101 64-65 0.8693278274815199 1101 66-67 0.8515871650996196 1101 68-69 0.902845939409481 1101 70-71 0.7008887340246197 1101 72-73 0.8056573450313742 1101 74-75 0.7122660393516398 1101 76-77 0.8624466898658518 1101 78-79 0.9416249820921969 1101 80-81 1.191385952505719 1101 82-83 0.961279485695254 1101 84-85 0.6844816101483318 1101 86-87 -0.07401953657955218 1101 88-89 0.35942464709398436 1101 90-91 0.13825375656028527 1101 92-93 0.2866642186153605 1101 94-95 0.5923859430394991 1101 96-97 0.10898960640846411 1101 98-99 0.5611557569837053 1101 100-101 -0.35975291623472927 1102 1 0.11709008139543187 1102 2 0.19526623979979973 1102 3 0.22457875009219208 1102 4 0.15088083624947046 1102 5 -0.005096794108098379 1102 6 0.2526583647670506 1102 7 0.16451598462953143 1102 8 0.08250117219630226 1102 9 0.4489574578034805 1102 10-11 0.23419035241186492 1102 12-13 0.09121942629518287 1102 14-15 0.213427318985417 1102 16-17 0.2122686066131294 1102 18-19 0.30706554761125915 1102 20-21 0.4010552073195299 1102 22-23 0.4587023949430531 1102 24-25 0.3821580681958707 1102 26-27 0.3387012382019776 1102 28-29 0.20922014442469816 1102 30-31 0.30517576912949806 1102 32-33 0.16874359608062406 1102 34-35 0.6170109276234825 1102 36-37 0.5460183006219097 1102 38-39 0.3996509825403223 1102 40-41 0.7618896019222561 1102 42-43 0.6494814400365314 1102 44-45 0.527131254675858 1102 46-47 0.5360041032757294 1102 48-49 0.5451337357875943 1102 50-51 1.1818052725686385 1102 52-53 1.032635419017744 1102 54-55 0.8944694815986267 1102 56-57 1.123901344079087 1102 58-59 0.8690225333583008 1102 60-61 0.8785582632733657 1102 62-63 1.0642312054753553 1102 64-65 0.5925900647072382 1102 66-67 0.5486018105567965 1102 68-69 1.0604721477875714 1102 70-71 1.167800059958502 1102 72-73 0.9408422460594572 1102 74-75 0.4712255128653595 1102 76-77 0.3985190135321588 1102 78-79 0.8054341649131977 1102 80-81 0.6143054962630714 1102 82-83 0.7405320388468404 1102 84-85 0.600116786524648 1102 86-87 0.40874775580105904 1102 88-89 0.25196637556482315 1102 90-91 0.4259287262975562 1102 92-93 0.2575395361609232 1102 94-95 0.2160624813239842 1102 96-97 -0.8984780423051575 1102 98-99 -0.4408134517524154 1102 100-101 -0.9865537618862739 1103 1 -0.10885049258286728 1103 2 0.020655821654237627 1103 3 0.06965869465494734 1103 4 -0.03143671099049783 1103 5 0.19043780346483175 1103 6 0.1842575940541451 1103 7 -0.02358190943125038 1103 8 0.24801853013836705 1103 9 0.12902709239091337 1103 10-11 0.20099561218943762 1103 12-13 0.1403439234726136 1103 14-15 0.18275936779723168 1103 16-17 0.1955191221118895 1103 18-19 0.20717709816394603 1103 20-21 0.3612434911311766 1103 22-23 0.3413630446405165 1103 24-25 0.29469229393362895 1103 26-27 0.5408950657380629 1103 28-29 0.4028026073256896 1103 30-31 0.5574236817820335 1103 32-33 0.8012436805886125 1103 34-35 0.6246335487234376 1103 36-37 0.6552533342560949 1103 38-39 0.6204534704556863 1103 40-41 0.7440668658914191 1103 42-43 0.8394131575770132 1103 44-45 0.9232624786896579 1103 46-47 1.0563198251430137 1103 48-49 1.0763763413380687 1103 50-51 0.9614675786227949 1103 52-53 1.1529494507251385 1103 54-55 1.1407680314414463 1103 56-57 1.1738286672038498 1103 58-59 1.3022105129411692 1103 60-61 1.05948987940425 1103 62-63 1.3698797198248123 1103 64-65 1.2087294014884975 1103 66-67 1.7240235054491357 1103 68-69 1.3920815180339936 1103 70-71 0.6935158426715446 1103 72-73 0.6894732165492634 1103 74-75 0.5339642479496973 1103 76-77 0.9563478341385867 1103 78-79 1.0868035324553666 1103 80-81 0.7465436059882506 1103 82-83 0.9092184465810114 1103 84-85 1.077713717194289 1103 86-87 1.3397976831241856 1103 88-89 1.1636217181602326 1103 90-91 1.458616418553241 1103 92-93 0.9770744041594348 1103 94-95 0.2906745896294325 1103 96-97 0.7293994549060798 1103 98-99 0.40948063606829166 1103 100-101 -0.2915515646784179 1104 1 0.0732304322726236 1104 2 0.21329605612464775 1104 3 0.2775150815311207 1104 4 0.14449750703441566 1104 5 0.27695575510530546 1104 6 0.1733156417623576 1104 7 0.2848932187947568 1104 8 0.3159498172831974 1104 9 0.38976958201071454 1104 10-11 0.4170706752847835 1104 12-13 0.036615192706513255 1104 14-15 0.4138185552921456 1104 16-17 0.35780439443949774 1104 18-19 0.5117850929534953 1104 20-21 0.12459527815389038 1104 22-23 0.1810316923650106 1104 24-25 0.3881995804271625 1104 26-27 0.37565041866486837 1104 28-29 0.38121586141671315 1104 30-31 0.11722584737675845 1104 32-33 0.5296487933460838 1104 34-35 0.7489028640372837 1104 36-37 0.7110458930772268 1104 38-39 1.0385028069370037 1104 40-41 1.1128285690584008 1104 42-43 1.275405993203492 1104 44-45 1.2688905825730714 1104 46-47 1.4806379884581702 1104 48-49 1.6923374257637036 1104 50-51 1.930693337802147 1104 52-53 1.6366301476233005 1104 54-55 1.8271109444105846 1104 56-57 1.753178174653172 1104 58-59 1.9461207954454416 1104 60-61 1.971396579721759 1104 62-63 2.0197291051541306 1104 64-65 2.351670865300264 1104 66-67 1.8908882778988634 1104 68-69 1.5482038008639236 1104 70-71 1.5932716026650304 1104 72-73 1.7794634469489985 1104 74-75 1.5424922948437754 1104 76-77 1.5919091790868904 1104 78-79 2.083673189705223 1104 80-81 1.9261414241108596 1104 82-83 1.8118358853174747 1104 84-85 1.4161245288852413 1104 86-87 1.4463270326316326 1104 88-89 1.4369140734480297 1104 90-91 1.0477803035976123 1104 92-93 1.465615477187196 1104 94-95 1.0654323849563063 1104 96-97 0.7587001143540988 1104 98-99 0.8360335954430376 1104 100-101 0.3892348881425569 1105 1 0.00260896334606997 1105 2 -0.03424114448011295 1105 3 -0.15340717517294422 1105 4 0.01741864970967555 1105 5 -0.03909421704397431 1105 6 -0.26146592901825727 1105 7 -0.1214885747916199 1105 8 -0.0677614901091772 1105 9 0.16057406995533086 1105 10-11 0.03657413673142429 1105 12-13 -0.1715172175743831 1105 14-15 -0.053051023764112415 1105 16-17 -0.20831321517298562 1105 18-19 -0.3417599058529035 1105 20-21 0.1487259378180923 1105 22-23 0.08497853727628524 1105 24-25 -0.19493084051658371 1105 26-27 -0.13904680422046312 1105 28-29 -0.02965387678651865 1105 30-31 0.06317953560783707 1105 32-33 0.11234553879049969 1105 34-35 0.1252377092668553 1105 36-37 0.35360577212552613 1105 38-39 0.34652381433733126 1105 40-41 0.30426248327818683 1105 42-43 0.3843659677399742 1105 44-45 0.3186120753716679 1105 46-47 0.5447264741747304 1105 48-49 0.588720810819801 1105 50-51 0.49900717563138386 1105 52-53 0.5153641334592223 1105 54-55 0.7805087322676698 1105 56-57 0.7702984101906907 1105 58-59 0.3821556486323594 1105 60-61 0.6209509681995264 1105 62-63 0.40395764020624725 1105 64-65 -0.10923865964602086 1105 66-67 -0.25341028182104 1105 68-69 -0.19740772137686946 1105 70-71 0.15704577311143808 1105 72-73 -0.05469745063992093 1105 74-75 0.09586621065503209 1105 76-77 0.19379109203349643 1105 78-79 0.36210479629390946 1105 80-81 0.8523370157397281 1105 82-83 1.1645334264967566 1105 84-85 0.9171947922734809 1105 86-87 0.6543122319632175 1105 88-89 0.4717354309717017 1105 90-91 0.5712107165840052 1105 92-93 0.423859290348652 1105 94-95 -0.0778715061871651 1105 96-97 -0.35119882731853735 1105 98-99 -0.5040754202329403 1105 100-101 -1.228326980242901 1106 1 0.2215820806242732 1106 2 0.2700364987805841 1106 3 0.22509285586945538 1106 4 0.11942398900314544 1106 5 0.1037008410053204 1106 6 0.033793895902583415 1106 7 0.1275646318887027 1106 8 0.25414823858021407 1106 9 0.07992590456140647 1106 10-11 -0.0464953361685545 1106 12-13 0.10422469587939531 1106 14-15 0.04966053021863104 1106 16-17 0.1988777576959606 1106 18-19 -0.02421939551578589 1106 20-21 0.09607802076339311 1106 22-23 -2.1734029247255648E-4 1106 24-25 0.22708288022595013 1106 26-27 0.15676402337002315 1106 28-29 0.1999823062394981 1106 30-31 0.26610373005745913 1106 32-33 0.3456602466814829 1106 34-35 0.4068621582641825 1106 36-37 0.6198487099786334 1106 38-39 0.8177475059000017 1106 40-41 0.13368447371712477 1106 42-43 0.38352006223304613 1106 44-45 0.777372241758961 1106 46-47 0.5749074513895991 1106 48-49 0.891849885135322 1106 50-51 0.7297376033957121 1106 52-53 0.6417222386308836 1106 54-55 0.6992940430021335 1106 56-57 0.5593730361297204 1106 58-59 0.8782443057379687 1106 60-61 0.8340881135400515 1106 62-63 0.811893630769358 1106 64-65 0.85979654244003 1106 66-67 1.0673586385241514 1106 68-69 0.4136387180594028 1106 70-71 0.5488086712302724 1106 72-73 0.2688497648564443 1106 74-75 0.19208214161672288 1106 76-77 0.5325801278564306 1106 78-79 0.7125657759921786 1106 80-81 0.43477011935927123 1106 82-83 0.5654227913691692 1106 84-85 0.3861523883497213 1106 86-87 0.38687416155904586 1106 88-89 0.4068568067710423 1106 90-91 0.6737437767441854 1106 92-93 0.5041737499003993 1106 94-95 0.4186362233714114 1106 96-97 0.025562829104138984 1106 98-99 0.06017459528826308 1106 100-101 -0.3111713957144353 1107 1 -0.034296449447808186 1107 2 -0.005309123339230837 1107 3 -0.08460819576707479 1107 4 0.006757086013664093 1107 5 0.056495358315970634 1107 6 0.1962067518638264 1107 7 -0.04412389463853117 1107 8 0.03416123633514445 1107 9 0.35293783886366015 1107 10-11 0.12897435543016655 1107 12-13 0.2154884321431325 1107 14-15 0.26226244443344626 1107 16-17 0.26478228327790276 1107 18-19 0.1814996166548397 1107 20-21 0.2611051978873462 1107 22-23 0.1166442926981901 1107 24-25 0.18599225042564882 1107 26-27 0.18599420179375414 1107 28-29 0.3936193143926303 1107 30-31 0.006340643681468805 1107 32-33 0.22005466946300345 1107 34-35 0.11910958825355067 1107 36-37 0.15479494653776982 1107 38-39 0.1912704882939522 1107 40-41 0.3734487417072003 1107 42-43 0.06916640513745875 1107 44-45 0.2508154652021801 1107 46-47 0.3766473781295261 1107 48-49 0.6441244922486362 1107 50-51 0.41963775700553896 1107 52-53 0.1306593767615638 1107 54-55 0.49230479569030905 1107 56-57 0.4506793105005116 1107 58-59 0.07230964899685688 1107 60-61 0.0775301553691925 1107 62-63 -0.11410778716754422 1107 64-65 0.22600828768403147 1107 66-67 0.6150515370235006 1107 68-69 0.27290848055497463 1107 70-71 0.24839806033579137 1107 72-73 0.23505560203002673 1107 74-75 -0.08574487008125686 1107 76-77 0.25420661964421143 1107 78-79 0.6492608013968812 1107 80-81 0.30827833157716356 1107 82-83 0.11282028634731134 1107 84-85 0.45795021417980664 1107 86-87 0.17037292086425637 1107 88-89 -0.06860993079246924 1107 90-91 -0.03153213014462608 1107 92-93 0.06719809115054787 1107 94-95 0.19957974221815888 1107 96-97 -0.12739711417838606 1107 98-99 0.2897326716205306 1107 100-101 -0.04225080018093408 1108 1 -0.01936216031996807 1108 2 -0.14001641127232745 1108 3 0.01711564789225406 1108 4 -0.23731186773270707 1108 5 -0.2699255326210519 1108 6 0.04366365577234177 1108 7 0.0144185187425947 1108 8 -0.07246958803761316 1108 9 -0.36390803927253756 1108 10-11 -0.05249859217181552 1108 12-13 -0.17207770542300693 1108 14-15 -0.0960964905383932 1108 16-17 -0.10957767575947486 1108 18-19 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-0.8359823213139848 2316 90-91 -0.9210589615011777 2316 92-93 -0.8393454358319019 2316 94-95 -0.23777703550086216 2316 96-97 -0.29609092697585027 2316 98-99 0.4360302969636365 2316 100-101 0.06819718652300111 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 7.0 5 34.0 6 112.0 7 197.0 8 297.0 9 513.0 10 846.0 11 1321.0 12 1950.0 13 2742.0 14 3159.0 15 4426.0 16 5716.0 17 8015.0 18 7383.0 19 4732.0 20 4165.0 21 4371.0 22 4693.0 23 5236.0 24 5954.0 25 6389.0 26 7139.0 27 8000.0 28 9328.0 29 10852.0 30 12668.0 31 14855.0 32 17656.0 33 21607.0 34 29251.0 35 46017.0 36 46015.0 37 55396.0 38 29019.0 39 1093.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.05657001526075 25.232594853444194 17.584855022891123 17.125980108403937 2 14.819236016371079 26.355336341693775 40.12199601217337 18.70343162976178 3 16.76907335502151 33.73806275579809 32.01726309161507 17.475600797565328 4 11.015321649700912 22.562965683702384 39.72085213558611 26.7008605310106 5 9.363259523559659 42.3391751495435 34.89689369293735 13.400671633959494 6 23.18737538041767 42.94233392800923 18.506139154160984 15.364151537412111 7 21.630286493860844 38.56989190890964 24.696977647182287 15.102843950047223 8 23.384930213033897 36.07986147549585 22.337863364466365 18.19734494700388 9 25.78077447790954 18.394112708573825 23.52083114702487 32.30428166649176 10-11 21.41790848987302 31.32962535418197 27.8611344317347 19.391331724210307 12-13 24.200335816979745 30.368742785182075 28.958180291741005 16.47274110609718 14-15 21.458695991531016 30.832560464167514 29.706642039797405 18.00210150450407 16-17 16.16794195544863 33.93798594012155 31.233971436854514 18.660100667575307 18-19 15.68881834400252 30.364676251442962 37.285654318396475 16.660851086158043 20-21 16.94800083954245 29.921817609402872 37.790429216077236 15.339752334977439 22-23 17.126272431524818 29.56107671319131 37.800267604155735 15.512383251128133 24-25 16.371294843608162 30.740401618226564 37.60710781355763 15.281195724607644 26-27 15.74327080870981 30.804669473037976 37.98557810243299 15.466481615819225 28-29 15.619949627453039 29.880234022457756 38.9531955084479 15.546620841641307 30-31 16.023870055385455 30.734729644992616 37.743836367595705 15.497563932026226 32-33 15.50057718543394 31.441782978276837 37.72969356700598 15.32794626928324 34-35 15.813474493443794 30.915113708667047 37.87931860073073 15.392093197158433 36-37 15.968939152387362 30.824532191251475 37.102833292001094 16.103695364360064 38-39 15.69014627797407 31.115918963917927 37.67513930454657 15.518795453561435 40-41 15.461959088571458 31.050126470680844 38.077252070235836 15.410662370511865 42-43 15.587979859731796 31.135553240783253 36.95605401873916 16.32041288074579 44-45 15.653659118904475 31.254583163343614 36.41343773653714 16.67831998121477 46-47 16.372210780391182 31.124214558381762 35.86756044129029 16.636014219936772 48-49 16.175291906123043 31.68498144472706 35.22051849821006 16.91920815093983 50-51 16.3329048168748 31.736147549585475 34.756926225207266 17.17402140833246 52-53 16.193483305545318 31.983592170731672 34.98758393795774 16.835340585765277 54-55 16.622678140413473 31.81223108405919 34.436325952355965 17.128764823171373 56-57 16.650094448525554 32.28604785391961 33.45130653793682 17.612551159618008 58-59 16.12356484688202 31.79139642759692 34.53668429779821 17.548354427722856 60-61 16.763593476757897 32.67104750485071 33.41711937805916 17.14823964033222 62-63 16.51952270258641 32.68209557492405 34.145445577285344 16.6529361452042 64-65 16.707223486662162 31.954139091308598 34.06707289077207 17.271564531257173 66-67 16.518932959025047 32.283958310103046 33.47181865288373 17.72529007798818 68-69 16.327561327561327 32.09628755083301 33.97113997113997 17.605011150465696 70-71 16.88190901709127 31.47907896825345 33.9410404305809 17.697971584074384 72-73 16.318999895057193 31.421187952565848 33.666570469094346 18.59324168328261 74-75 16.12629866722636 32.194091720012594 33.143692937349144 18.5359166754119 76-77 15.830773941647184 31.55680666769174 34.47970425391268 18.132715136748395 78-79 15.812015704711543 30.6960734285778 34.40921713875687 19.082693727953785 80-81 15.842747923367947 32.07104364206892 33.40098020181243 18.685228232750706 82-83 16.251964413614335 30.91416397967242 33.475969220032695 19.357902386680554 84-85 15.445875747717494 30.700230874173574 33.695298562283554 20.158594815825374 86-87 15.695385193072472 31.161795926512852 33.608435293051855 19.534383587362818 88-89 15.897061940006765 30.341086638612435 33.82763890333136 19.93421251804944 90-91 15.815671817603913 30.097907730617518 33.759938509891455 20.32648194188711 92-93 16.066087732185956 30.327421555252386 33.27277258893903 20.333718123622628 94-95 15.393929058663028 29.520673732815617 33.90636478119425 21.179032427327108 96-97 15.666911533214398 29.60174205058243 33.56215237695456 21.16919403924861 98-99 15.473121791819327 29.68402576907789 33.32743021530639 21.51542222379639 100-101 15.485613539827419 28.726482787932532 34.40012908066755 21.3877745915725 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8176.0 1 4881.5 2 1809.0 3 1920.5 4 1458.0 5 1295.5 6 2262.5 7 4454.5 8 6314.0 9 6292.5 10 4447.0 11 2734.0 12 2293.5 13 2118.5 14 2032.5 15 1946.0 16 1841.0 17 1764.5 18 1737.0 19 1737.0 20 1772.0 21 1867.5 22 1929.0 23 2039.0 24 2135.0 25 2254.0 26 2540.5 27 2950.5 28 3146.5 29 3650.5 30 4958.5 31 6192.5 32 8297.0 33 11099.0 34 12750.0 35 14921.0 36 19700.0 37 23274.0 38 22894.0 39 21354.5 40 19284.0 41 16290.5 42 13343.5 43 12605.0 44 12688.5 45 12103.0 46 10339.5 47 8613.5 48 9260.5 49 8165.5 50 5907.0 51 4409.0 52 2886.5 53 2075.5 54 1646.0 55 1442.5 56 1330.0 57 1276.0 58 1351.5 59 1467.0 60 1604.0 61 1662.5 62 1359.5 63 831.0 64 476.0 65 328.5 66 257.0 67 202.0 68 147.0 69 115.5 70 92.0 71 75.5 72 61.5 73 62.0 74 60.0 75 50.5 76 39.5 77 27.0 78 21.0 79 17.0 80 13.0 81 8.5 82 5.5 83 4.0 84 1.5 85 1.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28859271696925176 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011806065694196662 16-17 0.0011806065694196662 18-19 0.0 20-21 0.0 22-23 0.0 24-25 5.24714030853185E-4 26-27 9.182495539930737E-4 28-29 0.0 30-31 0.0034106412005457027 32-33 0.0 34-35 0.008526603001364257 36-37 0.02662923706579914 38-39 0.01849616958757477 40-41 0.011543708678770071 42-43 0.008657781509077552 44-45 0.002492391646552629 46-47 0.0013117850771329626 48-49 3.9353552313988875E-4 50-51 0.0 52-53 1.3117850771329626E-4 54-55 0.0 56-57 0.0 58-59 0.00419771224682548 60-61 0.008001888970511071 62-63 0.003672998215972295 64-65 0.001967677615699444 66-67 0.0032794626928324064 68-69 0.002623570154265925 70-71 1.3117850771329626E-4 72-73 0.0 74-75 0.0 76-77 0.18325637527547486 78-79 1.3117850771329626E-4 80-81 0.003672998215972295 82-83 7.870710462797775E-4 84-85 0.0 86-87 0.08697135061391542 88-89 0.3411952985622836 90-91 0.33096337496064643 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.001442963584846259 100-101 7.870710462797775E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 381160.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.177615699443802 #Duplication Level Percentage of deduplicated Percentage of total 1 72.71145289526608 18.3070101794522 2 10.614065251596903 5.344737118270543 3 4.166015401127471 3.146710043026551 4 2.292454697969094 2.308741735754014 5 1.5296924984630134 1.9257004932311887 6 1.0388987881250846 1.5694196662818762 7 0.8419560890722853 1.4838912792528072 8 0.6366771911177801 1.2824010914051842 9 0.5189283816311857 1.1758841431419875 >10 4.59220356997718 23.790796515898833 >50 0.6721060364500298 11.848567530695771 >100 0.3678347765377682 15.903557561129183 >500 0.00729417403899257 1.0740896211564699 >1k 0.0062521491762793455 3.0155315353132544 >5k 0.004168099450852897 7.822961485990135 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9170 2.4058138314618533 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7568 1.985517892748452 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7229 1.8965788645188373 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5851 1.5350508972609926 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3841 1.0077132962535418 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2227 0.5842690733550214 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1618 0.4244936509602267 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1529 0.40114387658726 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1204 0.3158778465736174 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1075 0.28203379158358693 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 644 0.16895791793472556 No Hit ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT 622 0.16318606359534055 RNA PCR Primer, Index 26 (100% over 22bp) GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 603 0.1582012803022353 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 578 0.15164235491657047 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 573 0.1503305698394375 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 569 0.14928114177773114 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 505 0.13249029279042923 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 498 0.13065379368244306 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 485 0.12724315248189738 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 477 0.12514429635848462 No Hit CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 456 0.11963479903452619 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 446 0.11701122888026025 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 440 0.1154370867877007 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 412 0.1080910903557561 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 402 0.1054675202014902 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 386 0.10126980795466471 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.24714030853185E-4 0.0 18-19 0.0 0.0 0.0 7.870710462797775E-4 0.0 20-21 0.0 0.0 0.0 9.182495539930737E-4 0.0 22-23 0.0 0.0 0.0 0.0020988561234127397 0.0 24-25 0.0 0.0 0.0 0.0040665337391121836 0.0 26-27 0.0 0.0 0.0 0.0076083534473711835 0.0 28-29 0.0 0.0 0.0 0.017053206002728513 0.0 30-31 0.0 0.0 0.0 0.04119005142197502 0.0 32-33 0.0 0.0 0.0 0.07687060551999161 0.0 34-35 0.0 0.0 0.0 0.13144086472872285 0.0 36-37 0.0 0.0 0.0 0.2127715395109665 0.0 38-39 0.0 0.0 0.0 0.3261097701752545 0.0 40-41 0.0 0.0 0.0 0.45348410116486515 0.0 42-43 0.0 0.0 0.0 0.5664287963060133 0.0 44-45 0.0 0.0 0.0 0.7107251547906391 0.0 46-47 0.0 0.0 0.0 0.8762724315248189 0.0 48-49 0.0 0.0 0.0 1.047591562598384 0.0 50-51 0.0 0.0 0.0 1.2274372966733131 0.0 52-53 0.0 0.0 0.0 1.4240738797355441 0.0 54-55 0.0 0.0 0.0 1.631729457445692 0.0 56-57 0.0 0.0 0.0 1.876115017315563 0.0 58-59 0.0 0.0 0.0 2.143194459019834 0.0 60-61 0.0 0.0 0.0 2.438477279882464 0.0 62-63 0.0 0.0 0.0 2.7370395634379263 0.0 64-65 0.0 0.0 0.0 3.0555409801658096 0.0 66-67 0.0 0.0 0.0 3.4166754119005143 0.0 68-69 0.0 0.0 0.0 3.818475181026341 0.0 70-71 0.0 0.0 0.0 4.2665809633749605 0.0 72-73 0.0 0.0 0.0 4.7169167803547065 0.0 74-75 0.0 0.0 0.0 5.18981530066114 0.0 76-77 0.0 0.0 0.0 5.683177668170847 0.0 78-79 0.0 0.0 0.0 6.282138734389758 0.0 80-81 0.0 0.0 0.0 6.93448945324798 0.0 82-83 0.0 0.0 0.0 7.5653268968412215 0.0 84-85 0.0 0.0 0.0 8.224498898100535 0.0 86-87 0.0 0.0 0.0 8.903478854024556 0.0 88-89 0.0 0.0 0.0 9.603447371182707 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACCGAT 50 2.8390841E-5 47.45553 7 AGAACCG 70 4.3303826E-6 40.676167 5 GGATTTA 60 8.16649E-5 39.67639 1 GAACCGA 65 1.333849E-4 36.504253 6 GGTACTC 170 0.0 36.289524 3 TGGTACT 175 0.0 35.25268 2 GCTTACG 55 0.0025479498 34.626675 1 ACCGATT 55 0.0025893378 34.513115 8 TATAGGT 55 0.0025893378 34.513115 3 CTTACGC 55 0.0025893378 34.513115 2 GGTATCA 3990 0.0 32.695736 1 CGGAGGT 75 3.0891763E-4 31.64117 9 GCAGATC 75 3.091555E-4 31.63702 3 CTTAAGT 60 0.003965811 31.637018 8 ACGCCAA 60 0.003965811 31.637018 5 GGGAGAT 60 0.003965811 31.637018 7 GGGACTA 60 0.003965811 31.637018 7 CCCAACG 60 0.003965811 31.637018 7 ACTACCT 165 7.6397555E-11 31.637018 4 GGACAAT 230 0.0 30.949259 6 >>END_MODULE